FastQCFastQC Report
Fri 27 May 2016
SRR939207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338054
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232341.736402267770957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164411.2287247001989456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120850.9031773007666357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78680.5880181218396268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40390.3018562778482782No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37050.27689465447582834No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34450.25746345065296317No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33440.24991517532177326No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.23377232906893144No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.17749657338194125No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21510.16075584393454972No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19820.1481255614496874No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19100.14274461269874011No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15360.11479357335354179No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14400.10761897501894542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA207400.030.0750641
GGTATCA142450.027.2590521
TATCAAC247650.025.0988142
GAGTACT125750.024.87668212-13
ATCAACG250450.024.6857973
TCAACGC251900.024.5625084
TACATGG144150.024.5506862
GTACATG145800.024.451321
CAACGCA252800.024.3626175
AACGCAG256250.024.0652876
AGTACTT126750.023.65284712-13
ACATGGG148000.022.9517143
CGTCACT2704.0017767E-1122.8559631
GTACTTT136750.022.84185614-15
ACGCAGA279850.021.9512167
CGCAGAG281750.021.7695648
TACCTGG9150.021.7463232
CATGGGG35650.021.528474
GCAGAGT286300.021.0753969
TAGCGCG1353.8640332E-421.0559654
Produced by FastQC (version 0.11.5)