##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939207_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1338054 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.211659619118514 33.0 31.0 34.0 31.0 34.0 2 32.26239822906998 34.0 31.0 34.0 31.0 34.0 3 32.28140344111672 34.0 31.0 34.0 30.0 34.0 4 35.76784569232632 37.0 35.0 37.0 35.0 37.0 5 35.83300375022234 37.0 35.0 37.0 35.0 37.0 6 35.95660713244757 37.0 37.0 37.0 35.0 37.0 7 35.89581960070371 37.0 37.0 37.0 35.0 37.0 8 35.893994562252345 37.0 37.0 37.0 35.0 37.0 9 37.556330312528495 39.0 38.0 39.0 35.0 39.0 10-11 37.484061181387304 39.0 38.0 39.0 35.0 39.0 12-13 37.38159521215138 39.0 37.5 39.0 35.0 39.0 14-15 38.59622369500782 40.0 38.0 41.0 34.5 41.0 16-17 38.574900938228204 40.0 38.0 41.0 34.5 41.0 18-19 38.611610966373554 40.0 38.0 41.0 34.0 41.0 20-21 38.57314727208319 40.0 38.0 41.0 34.0 41.0 22-23 38.46820419803685 40.0 38.0 41.0 34.0 41.0 24-25 38.456653468395146 40.0 38.0 41.0 34.0 41.0 26-27 38.33690419071279 40.0 38.0 41.0 34.0 41.0 28-29 38.175433502683745 40.0 38.0 41.0 34.0 41.0 30-31 37.861184974597435 40.0 37.0 41.0 33.5 41.0 32-33 37.49835731592297 40.0 35.5 41.0 33.0 41.0 34-35 37.11643364169159 40.0 35.0 41.0 32.5 41.0 36-37 36.803575939386604 40.0 35.0 41.0 31.5 41.0 38-39 36.50908408778719 39.0 35.0 41.0 31.0 41.0 40-41 36.270448352607595 39.0 35.0 41.0 30.5 41.0 42-43 35.951198905275874 39.0 35.0 41.0 30.0 41.0 44-45 35.48134903374602 39.0 35.0 41.0 28.0 41.0 46-47 34.82791912732969 38.5 35.0 40.5 23.5 41.0 48-49 34.50245954199158 39.0 35.0 41.0 21.0 41.0 50-51 33.425423039727846 38.0 34.0 40.0 17.0 40.5 52-53 33.561146635337586 38.0 33.5 39.5 17.0 40.5 54-55 33.998405146578534 38.0 35.0 40.5 15.5 41.0 56-57 33.85216403822267 38.0 34.5 41.0 12.5 41.0 58-59 33.65514321544572 38.0 34.0 40.5 9.0 41.0 60-61 33.340571083080356 37.5 34.0 40.0 7.0 41.0 62-63 32.97747026652138 37.0 34.0 40.0 2.0 41.0 64-65 32.60198878371127 36.0 33.0 40.0 2.0 41.0 66-67 32.199818916127455 36.0 33.0 39.0 2.0 41.0 68-69 31.792703059816716 35.0 33.0 39.0 2.0 41.0 70-71 31.37280184506754 35.0 32.0 38.5 2.0 40.5 72-73 30.933480263128395 35.0 32.0 37.5 2.0 40.0 74-75 30.486900379207416 35.0 31.0 37.0 2.0 39.0 76-77 30.05193288163258 35.0 31.0 36.5 2.0 39.0 78-79 29.63212583348654 35.0 30.5 36.0 2.0 38.5 80-81 29.206383299926607 35.0 30.0 35.5 2.0 37.0 82-83 28.778746597670946 34.5 29.0 35.0 2.0 37.0 84-85 28.402081306135628 34.0 29.0 35.0 2.0 36.5 86-87 28.005556576939348 34.0 29.0 35.0 2.0 36.0 88-89 27.600288179699774 34.0 27.5 35.0 2.0 36.0 90-91 27.223427455095234 34.0 26.5 35.0 2.0 35.0 92-93 26.79850028474187 34.0 25.5 35.0 2.0 35.0 94-95 26.33644456800697 34.0 24.5 35.0 2.0 35.0 96-97 25.787645715344823 34.0 21.5 35.0 2.0 35.0 98-99 25.129653960154073 33.0 10.0 35.0 2.0 35.0 100-101 22.82332588968756 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24856640274582276 1101 2 0.3791126559926852 1101 3 0.27798233885252444 1101 4 0.27603743506538336 1101 5 0.24800451641607424 1101 6 0.16738465134748992 1101 7 0.22480211158836028 1101 8 0.3010663582080326 1101 9 0.45762425443631116 1101 10-11 0.4891006194955807 1101 12-13 0.31146789099953054 1101 14-15 0.4026864730662325 1101 16-17 0.4144513407952459 1101 18-19 0.4263309708321614 1101 20-21 0.345099814547531 1101 22-23 0.40843721232619856 1101 24-25 0.4887490882303922 1101 26-27 0.43888129003771326 1101 28-29 0.5222601339952035 1101 30-31 0.48338343021912067 1101 32-33 0.555434956542193 1101 34-35 0.4420596921524478 1101 36-37 0.5423965489781892 1101 38-39 0.5484554720094863 1101 40-41 0.4178763354398427 1101 42-43 0.5012751160986255 1101 44-45 0.5873518655297758 1101 46-47 0.9323663180427033 1101 48-49 0.8185026542492082 1101 50-51 0.8224207707628395 1101 52-53 1.0130633288849538 1101 54-55 0.9902170957330725 1101 56-57 0.7811090498171112 1101 58-59 0.8277606737171794 1101 60-61 0.9401454395814426 1101 62-63 0.9735236442214941 1101 64-65 1.0508777219465202 1101 66-67 0.8984629997110218 1101 68-69 0.5887666242522904 1101 70-71 0.6341354482261607 1101 72-73 0.6500135875100668 1101 74-75 0.5709491246277274 1101 76-77 0.44543396655923573 1101 78-79 0.5163280867254763 1101 80-81 0.38552725944022725 1101 82-83 0.5595859508989101 1101 84-85 0.5209297300746272 1101 86-87 0.5311583637783066 1101 88-89 0.49008513941410925 1101 90-91 0.4081701050979305 1101 92-93 0.4227348755713898 1101 94-95 0.37347356816013644 1101 96-97 0.4020632779815827 1101 98-99 0.20647004220084852 1101 100-101 -0.24377715907672481 1102 1 0.23519834719026278 1102 2 0.3054519417069699 1102 3 0.3774615055191859 1102 4 0.4317418001447493 1102 5 0.3737981672097277 1102 6 0.3692943735697085 1102 7 0.3244300877788362 1102 8 0.3665921518588249 1102 9 0.4853772306267885 1102 10-11 0.26433623457494093 1102 12-13 0.3673458671900107 1102 14-15 0.5095193103678142 1102 16-17 0.6610783249222365 1102 18-19 0.5467550779750212 1102 20-21 0.6819425724840471 1102 22-23 0.6997938591515975 1102 24-25 0.6217351993415079 1102 26-27 0.5739507344821533 1102 28-29 0.7195691617729878 1102 30-31 0.630841267520708 1102 32-33 0.7975108493993375 1102 34-35 0.7309361802476886 1102 36-37 0.6938598426289815 1102 38-39 0.6221409878824957 1102 40-41 0.49446363702714535 1102 42-43 0.4608113264160849 1102 44-45 0.4848717067996162 1102 46-47 0.4053945918522359 1102 48-49 0.38773628520158354 1102 50-51 0.32135430250887254 1102 52-53 0.4693753328532111 1102 54-55 0.5369308854156145 1102 56-57 0.379930974420283 1102 58-59 0.48523835228860435 1102 60-61 0.44718909037509036 1102 62-63 0.4659219577135545 1102 64-65 0.37580331718461224 1102 66-67 0.1898072457427631 1102 68-69 0.03803497742689643 1102 70-71 -0.021110087488125373 1102 72-73 -0.28795764264866364 1102 74-75 -0.22782319680084484 1102 76-77 -0.25828627153600436 1102 78-79 -0.2792200283538868 1102 80-81 -0.23546728421056784 1102 82-83 -0.27522307687886993 1102 84-85 -0.3247795953222017 1102 86-87 -0.33409461241216576 1102 88-89 -0.34964700344303523 1102 90-91 -0.4408626329973089 1102 92-93 -0.38902107680956277 1102 94-95 -0.36950758263351346 1102 96-97 -0.3749828529707955 1102 98-99 -0.4272105133547086 1102 100-101 -0.44428559161640635 1103 1 0.2962130752502006 1103 2 0.3481675721798254 1103 3 0.41138864358106986 1103 4 0.2120097267841743 1103 5 0.21374757370904973 1103 6 0.18400023803750543 1103 7 0.18810583938420677 1103 8 0.19731979828809187 1103 9 0.3370400662952022 1103 10-11 0.37574607233521107 1103 12-13 0.3722759970790932 1103 14-15 0.39556003448729626 1103 16-17 0.4474940916083554 1103 18-19 0.5221614374321106 1103 20-21 0.5058359916803852 1103 22-23 0.379818091735757 1103 24-25 0.42743669768749015 1103 26-27 0.4272295825070671 1103 28-29 0.38351232313706873 1103 30-31 0.39333229812004333 1103 32-33 0.40512769484592326 1103 34-35 0.40334706393127817 1103 36-37 0.2645858472018574 1103 38-39 0.5112533516692253 1103 40-41 0.3988863116720225 1103 42-43 0.3498545194011058 1103 44-45 0.15496194809169594 1103 46-47 0.11150457798766666 1103 48-49 0.06274677483964552 1103 50-51 0.16419976125570912 1103 52-53 0.06387252953543765 1103 54-55 0.1441935155479328 1103 56-57 0.15801203955941645 1103 58-59 0.024129825581070463 1103 60-61 0.11135040580611388 1103 62-63 0.12543210681844386 1103 64-65 0.17483553405560315 1103 66-67 -0.06843092072930546 1103 68-69 -0.04602274902766723 1103 70-71 -8.126378383863653E-4 1103 72-73 -0.00670004146166292 1103 74-75 -0.15193522613534682 1103 76-77 -0.09323409698842511 1103 78-79 -0.09877621024725158 1103 80-81 -0.10485380761006269 1103 82-83 -0.12639259551089665 1103 84-85 -0.015400642704957335 1103 86-87 -0.192926887660267 1103 88-89 0.01591232230127204 1103 90-91 -0.16029217911522764 1103 92-93 -0.23149407739333228 1103 94-95 -0.32015915638327286 1103 96-97 -0.15087173577873614 1103 98-99 -0.29303740088146313 1103 100-101 -0.2651674205228076 1104 1 0.2875184229478407 1104 2 0.270256234636264 1104 3 0.3208274566976428 1104 4 0.20241730519525447 1104 5 0.30814160155316017 1104 6 0.24789811936095418 1104 7 0.2568006685869122 1104 8 0.3146666468083197 1104 9 0.3101615319735842 1104 10-11 0.32887484989480953 1104 12-13 0.2621533166849588 1104 14-15 0.3054867977415583 1104 16-17 0.34642133839025036 1104 18-19 0.47992795255414933 1104 20-21 0.4513949083426212 1104 22-23 0.5785685855825733 1104 24-25 0.4260281286344352 1104 26-27 0.4026544381858628 1104 28-29 0.3982702350053131 1104 30-31 0.1645507582614556 1104 32-33 0.2413371330693721 1104 34-35 0.30541797738572996 1104 36-37 0.31888614734278775 1104 38-39 0.49106459899361 1104 40-41 0.20358611177461938 1104 42-43 0.04899787944639655 1104 44-45 0.0876705283484327 1104 46-47 -0.10795505040366038 1104 48-49 -0.07160977961323312 1104 50-51 -0.07797755776048376 1104 52-53 -0.19838297943635297 1104 54-55 -0.1912493544833751 1104 56-57 -0.19249905504099019 1104 58-59 -0.1022116687551673 1104 60-61 -0.09700282881682654 1104 62-63 -0.07412696905411309 1104 64-65 -0.23049403130023904 1104 66-67 -0.25929313304511226 1104 68-69 -0.377303301192633 1104 70-71 -0.34690712031640913 1104 72-73 -0.4330807487092727 1104 74-75 -0.2576132851846822 1104 76-77 -0.14806952069424995 1104 78-79 -0.053328298555907594 1104 80-81 -0.03182934229137757 1104 82-83 -0.11922297166001172 1104 84-85 -0.07664723000233309 1104 86-87 -0.14888312251317615 1104 88-89 -5.7356978388156676E-5 1104 90-91 0.13129119696902336 1104 92-93 -0.03517006670854883 1104 94-95 -0.11553557084900135 1104 96-97 -0.01577883361052912 1104 98-99 0.08641532839613575 1104 100-101 0.06801186208729959 1105 1 0.22193552754322354 1105 2 0.17141122047519985 1105 3 0.29050259817180546 1105 4 0.30212999121263806 1105 5 0.27394223348021285 1105 6 0.23977119915947753 1105 7 0.17045504050708615 1105 8 0.16475586966901545 1105 9 0.2917698900000971 1105 10-11 0.34627603625872183 1105 12-13 0.33568646011275405 1105 14-15 0.3720478703804204 1105 16-17 0.3553961486211392 1105 18-19 0.41653197222137806 1105 20-21 0.3803481359182186 1105 22-23 0.3544786860469671 1105 24-25 0.36273105743622835 1105 26-27 0.3641092073796841 1105 28-29 0.31792463966782236 1105 30-31 0.27697497340041366 1105 32-33 0.27533322581748365 1105 34-35 0.2164106703881501 1105 36-37 0.09968918037434804 1105 38-39 0.2888315718011043 1105 40-41 0.22422401430069527 1105 42-43 0.13442876856447583 1105 44-45 0.14897782061196807 1105 46-47 0.32752072299755497 1105 48-49 0.3352498758282749 1105 50-51 0.21694424263133527 1105 52-53 0.22260908600465967 1105 54-55 0.3147670494260524 1105 56-57 0.30767625287337097 1105 58-59 0.23305414442798877 1105 60-61 0.24867027171850964 1105 62-63 0.24970085538148368 1105 64-65 0.3437471651046593 1105 66-67 0.15199969802081625 1105 68-69 0.19658455085567184 1105 70-71 0.1553295903886962 1105 72-73 0.32629788364342716 1105 74-75 0.3370509421780845 1105 76-77 0.3526419429426575 1105 78-79 0.1670132858456448 1105 80-81 -0.07202874310666019 1105 82-83 0.02882217456179248 1105 84-85 0.313592962304309 1105 86-87 0.15362739596168495 1105 88-89 -0.03063869712212508 1105 90-91 -0.023094365844418263 1105 92-93 0.18825134350378292 1105 94-95 0.06734946535856423 1105 96-97 0.08493112933606284 1105 98-99 -0.11995823980887366 1105 100-101 -0.14026144363333515 1106 1 0.04199793140823971 1106 2 0.06809127291715811 1106 3 0.16396959999902094 1106 4 0.20205540594800908 1106 5 0.10141670931871971 1106 6 0.08561713542392368 1106 7 0.15316880858562598 1106 8 0.08714461116526451 1106 9 0.04968521894950584 1106 10-11 0.16370537381768457 1106 12-13 0.17916579818788847 1106 14-15 0.18298390254757635 1106 16-17 0.2232576419781438 1106 18-19 0.25343799994953997 1106 20-21 0.14172174357392464 1106 22-23 0.24779401396405376 1106 24-25 0.3768661264469486 1106 26-27 0.2592833601014064 1106 28-29 0.321941662657629 1106 30-31 0.33645156033740875 1106 32-33 0.31355881914456063 1106 34-35 0.3440017986128652 1106 36-37 0.3393227318717251 1106 38-39 0.32364598611321327 1106 40-41 0.3735661671049968 1106 42-43 0.374594611094075 1106 44-45 0.4378750684415067 1106 46-47 0.5511532834658155 1106 48-49 0.6760606615285454 1106 50-51 0.5482596015180761 1106 52-53 0.6484961086847747 1106 54-55 0.6704206899095979 1106 56-57 0.7649523164231127 1106 58-59 0.639771592734462 1106 60-61 0.7158108171974575 1106 62-63 0.8308342675154066 1106 64-65 0.6320566345943774 1106 66-67 0.7799689141787169 1106 68-69 0.6903731984106152 1106 70-71 0.7603638376711075 1106 72-73 0.7432204801397262 1106 74-75 0.657601763390236 1106 76-77 0.4890929747484982 1106 78-79 0.577232886543495 1106 80-81 0.8280915224065559 1106 82-83 0.8375716196887204 1106 84-85 0.9171535699290416 1106 86-87 0.9031499470358177 1106 88-89 0.9836531557926342 1106 90-91 0.9568450368540731 1106 92-93 0.8920962267997794 1106 94-95 0.8791378083785197 1106 96-97 1.1347122770716638 1106 98-99 1.1449652651307858 1106 100-101 1.0120434733163606 1107 1 0.023094522930719563 1107 2 0.013151286853350541 1107 3 0.13056323253510982 1107 4 0.01096892306890851 1107 5 0.08223850276871048 1107 6 0.17318203193300263 1107 7 0.1458179601465801 1107 8 0.06051496045324711 1107 9 0.24520404752458091 1107 10-11 0.16043907976412441 1107 12-13 0.29346216790906254 1107 14-15 0.3755946559850045 1107 16-17 0.30455493423570346 1107 18-19 0.20665853521693123 1107 20-21 0.22574861489972164 1107 22-23 0.29534082777854564 1107 24-25 0.2305778632689126 1107 26-27 0.23274203734125365 1107 28-29 0.2040397950121573 1107 30-31 0.2642268981918221 1107 32-33 0.262366637149718 1107 34-35 0.32774577513725234 1107 36-37 0.290216684351293 1107 38-39 0.30299748719767905 1107 40-41 0.18173625729765064 1107 42-43 0.3927104286674137 1107 44-45 0.3900730849882805 1107 46-47 0.5090055157094469 1107 48-49 0.3810453022791407 1107 50-51 0.3862765653919453 1107 52-53 0.44289562582530095 1107 54-55 0.41943374772515085 1107 56-57 0.34888227235728664 1107 58-59 0.32233749520077737 1107 60-61 0.405436821927907 1107 62-63 0.394392976160745 1107 64-65 0.30821937393859855 1107 66-67 0.17860277518978052 1107 68-69 0.35121778537930126 1107 70-71 0.40221546143329334 1107 72-73 0.3780175221347619 1107 74-75 0.5593915085150236 1107 76-77 0.3461452256391411 1107 78-79 0.17388022738143505 1107 80-81 0.22998370341997187 1107 82-83 0.47186445629182217 1107 84-85 0.3457618894406842 1107 86-87 0.42413519626785146 1107 88-89 0.30686782706544236 1107 90-91 0.46413945354609254 1107 92-93 0.4623046807661986 1107 94-95 0.3371979793421751 1107 96-97 0.29947825707029097 1107 98-99 0.42964993434260634 1107 100-101 0.3142097163359985 1108 1 -0.07237825374272688 1108 2 0.025036865371092176 1108 3 -0.06571995122381225 1108 4 -0.048901223604190136 1108 5 0.005774418269950843 1108 6 0.01080068188183958 1108 7 0.025483681926417034 1108 8 -0.009375299371029655 1108 9 0.058305824774372184 1108 10-11 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sequence quality scores pass #Quality Count 2 4028.0 3 806.0 4 837.0 5 987.0 6 1097.0 7 1379.0 8 1731.0 9 2218.0 10 2876.0 11 4038.0 12 6136.0 13 10374.0 14 9722.0 15 11542.0 16 15886.0 17 24108.0 18 21709.0 19 10543.0 20 9816.0 21 9963.0 22 10129.0 23 11007.0 24 11963.0 25 13082.0 26 14561.0 27 16583.0 28 19386.0 29 22593.0 30 26626.0 31 32129.0 32 39771.0 33 51550.0 34 72470.0 35 123289.0 36 171446.0 37 251638.0 38 252067.0 39 47958.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.73803408387859 18.949361630326777 14.882318753632426 19.43028553216221 2 15.376136986055366 21.74363302774261 40.95334728096089 21.926882705241134 3 19.430685661269564 28.78490407925059 30.054619459491818 21.729790799988027 4 12.066245938758499 17.451253877915075 37.12517228942529 33.35732789390114 5 12.36682183538146 39.601475849062254 31.909024233261984 16.1226780822943 6 28.55209732773607 36.82755803968152 17.86633294535488 16.75401168722753 7 26.459061009191814 36.77420995815918 19.98633983001841 16.780389202630595 8 26.918412616474583 33.16874459943217 19.49396445631478 20.418878327778465 9 28.945021932580506 16.684918882365967 20.448091895417797 33.92196728963574 10-11 25.12036803276389 28.125146100404873 25.05334417982739 21.701141687003844 12-13 27.510910497267325 26.38818030709478 26.685759725191616 19.41514947044628 14-15 23.87849794373367 26.071661676394736 28.136119407451183 21.913720972420407 16-17 20.079018152333596 29.998025294186288 28.216115095429156 21.706841458050967 18-19 19.978043064769217 26.719607692782454 33.68111572651459 19.62123351593374 20-21 22.035946288187553 25.30822193251046 33.52140074182006 19.134431037481928 22-23 21.01312478535016 25.979757257689794 33.45230265496128 19.554815301998758 24-25 20.40643595309197 26.243372899804644 33.92704867151173 19.423142475591654 26-27 20.076331022199607 26.1901839802138 34.271581286926065 19.461903710660525 28-29 19.666633616875597 26.75484101491543 34.08149330180508 19.49703206640389 30-31 19.832189682224364 26.690243013702116 33.804411343283135 19.673155960790385 32-33 19.52470519931078 27.010933119653803 33.58640441544508 19.877957265590336 34-35 20.193374764177115 26.83416964850768 33.44467122765869 19.527784359656515 36-37 20.313637720643875 26.601788621244527 33.38435453980462 19.70021911830699 38-39 20.03729492764206 26.88335818034992 33.3522673731704 19.727079518837616 40-41 19.886021557037267 27.098387951601012 33.2472408103332 19.768349681028514 42-43 19.81611755500253 27.41104000552691 32.48982853057748 20.28301390889307 44-45 19.99821264829199 27.39292974731503 32.25204813233012 20.356809472062867 46-47 20.320546762049606 27.858052160198277 31.389361422482587 20.43203965526953 48-49 20.383573353042667 27.950710144372366 30.95856157927035 20.707154923314622 50-51 20.22027788208787 28.499662035298535 30.64795343597447 20.63210664663913 52-53 20.540010124391824 28.799125456836894 30.02396656847709 20.636897850294194 54-55 19.933726966365967 28.564428465488273 30.223206826310207 21.278637741835556 56-57 20.370680425436706 28.76780724107329 29.744605834363192 21.116906499126813 58-59 20.58560360791814 28.30306092040046 29.94506752460814 21.16626794707326 60-61 20.460420523264148 28.404117139013096 30.517651366839427 20.61781097088333 62-63 20.551976332071845 28.41288970985763 30.3929386676278 20.64219529044272 64-65 20.289750025256232 28.60259983347786 30.427470669907976 20.680179471357928 66-67 20.36572009759146 28.62899469977405 29.961337495793472 21.043947706841017 68-69 20.382912324354212 28.645025723318067 30.081031456973545 20.891030495354176 70-71 20.355907521737155 28.70453733637272 29.833828943083567 21.105726198806554 72-73 20.229774113043007 28.527744341558957 29.939664577867998 21.302816967530042 74-75 19.828960054747206 29.210578800707175 29.702650108792046 21.257811035753576 76-77 19.927698274920044 28.72895189535739 29.899095270872362 21.4442545588502 78-79 19.837827467738006 28.79583628518428 29.96910552440078 21.397230722676934 80-81 19.826065017623268 29.117439902925597 29.71270392157157 21.34379115787956 82-83 19.829705487972706 28.746060587627216 29.642555003452674 21.781678920947403 84-85 19.727333283768488 28.520737254513218 29.602602604857804 22.149326856860487 86-87 19.73445714781382 28.425732227392942 29.687169672029267 22.152640952763967 88-89 19.726817902887447 28.258998349836222 29.541273998541367 22.472909748734963 90-91 19.639909329417048 28.155882789303575 29.821569757942594 22.382638123336783 92-93 19.710572432758475 28.09883398195575 29.861344282169906 22.329249303115866 94-95 19.5330909997799 27.83568192556769 30.010749513792955 22.62047756085945 96-97 19.503171703946254 27.533672945472382 30.149385021083972 22.81377032949739 98-99 19.644308469804567 27.50324120742676 29.787034268914304 23.06541605385437 100-101 19.861543747782054 26.79798793436565 30.53044854643467 22.81001977141763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7447.0 1 4780.5 2 2964.0 3 3720.0 4 2975.5 5 2745.5 6 4849.0 7 9411.5 8 14090.5 9 14488.5 10 10457.5 11 7180.0 12 6392.0 13 6328.0 14 6276.0 15 6095.0 16 5929.5 17 5754.0 18 5694.5 19 5753.0 20 5926.0 21 6271.0 22 6709.5 23 7496.0 24 8546.0 25 9539.5 26 10727.0 27 11970.5 28 13299.0 29 14848.0 30 16608.0 31 18459.0 32 20545.0 33 22288.5 34 23969.0 35 25215.0 36 27106.5 37 29946.5 38 31633.5 39 32219.0 40 32636.0 41 34552.5 42 36401.5 43 37479.0 44 38951.5 45 40573.5 46 41702.0 47 44700.0 48 54552.0 49 54352.0 50 45164.0 51 40971.0 52 37760.0 53 37358.0 54 36856.0 55 34251.0 56 31014.0 57 28181.5 58 26095.5 59 23730.0 60 20918.5 61 18646.0 62 16058.5 63 11909.5 64 8626.0 65 7093.5 66 5557.0 67 4537.0 68 3561.0 69 2483.0 70 1883.5 71 1423.0 72 1173.5 73 1027.0 74 784.5 75 634.5 76 500.0 77 308.5 78 186.5 79 141.5 80 96.5 81 69.5 82 55.0 83 38.5 84 26.5 85 21.5 86 14.5 87 13.0 88 15.0 89 11.5 90 7.5 91 5.0 92 2.0 93 1.0 94 3.5 95 3.0 96 3.0 97 2.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3422133934803827 2 0.12151975929222587 3 0.11464410255490436 4 0.12256605488268785 5 0.14005413832326646 6 0.09042983317564164 7 0.09872546250001869 8 0.1038074696536911 9 0.09192454116201589 10-11 0.09091561327121327 12-13 0.09476448633612694 14-15 0.0961470912235231 16-17 0.08564676761924407 18-19 0.10063121518264584 20-21 0.11034681709407841 22-23 0.11770825392697155 24-25 0.1329916430876482 26-27 0.1322069213948017 28-29 0.12604125095100796 30-31 0.11546619194741019 32-33 0.11008524319646293 34-35 0.33077887738461975 36-37 0.4918710306161037 38-39 0.4661620532504667 40-41 0.4454229799395242 42-43 0.47740973084793287 44-45 0.4839117105886608 46-47 0.49168419211780684 48-49 0.5011008524319647 50-51 0.48981580713483913 52-53 0.4944494018925992 54-55 0.48140807471148395 56-57 0.45977217660871683 58-59 0.4389210001987962 60-61 0.40215118373398984 62-63 0.47038460331197396 64-65 0.5006898077357117 66-67 0.5074533613740552 68-69 0.5206067916541485 70-71 0.513095884022618 72-73 0.5042023715036912 74-75 0.511526440636925 76-77 0.4895542332372236 78-79 0.46803043823343454 80-81 0.4702351325133365 82-83 0.48417328448627633 84-85 0.4831643565954737 86-87 0.48708796505970614 88-89 0.49855984885512844 90-91 0.512796942425343 92-93 0.5060333887869997 94-95 0.5105175127461223 96-97 0.5104054096471443 98-99 0.5052112993944937 100-101 0.4922447076126973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1338054.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.309347676413964 #Duplication Level Percentage of deduplicated Percentage of total 1 69.25635671907337 29.994476319480967 2 14.524020304669346 12.580516900684414 3 6.105439809367164 7.932678462639034 4 3.1766914696291386 5.5032174127546725 5 1.9299440751163406 4.179230947262439 6 1.2017892101559393 3.1229224041843904 7 0.8268433317325802 2.5067031729551563 8 0.5586574479964094 1.9356071717835721 9 0.4002428699832583 1.560083184999962 >10 1.8397278145609222 13.75346650952708 >50 0.10831983431289485 3.209352320157757 >100 0.06333797411109088 5.163427632811736 >500 0.005350066360733467 1.5999055675325304 >1k 0.0025887417874516775 2.501548214631102 >5k 1.7258278583011185E-4 0.5880895587641182 >10k+ 5.177483574903356E-4 3.8687742198310575 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23234 1.736402267770957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16441 1.2287247001989456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12085 0.9031773007666357 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7868 0.5880181218396268 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4039 0.3018562778482782 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3705 0.27689465447582834 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3445 0.25746345065296317 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3344 0.24991517532177326 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3128 0.23377232906893144 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2375 0.17749657338194125 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2151 0.16075584393454972 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1982 0.1481255614496874 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1910 0.14274461269874011 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1536 0.11479357335354179 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1440 0.10761897501894542 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.47353993187121E-5 0.0 3 0.0 0.0 0.0 7.47353993187121E-5 0.0 4 0.0 0.0 0.0 7.47353993187121E-5 0.0 5 0.0 0.0 0.0 7.47353993187121E-5 0.0 6 0.0 0.0 0.0 7.47353993187121E-5 0.0 7 0.0 0.0 0.0 7.47353993187121E-5 0.0 8 0.0 0.0 0.0 7.47353993187121E-5 0.0 9 0.0 0.0 0.0 7.47353993187121E-5 0.0 10-11 0.0 0.0 0.0 1.8683849829678024E-4 0.0 12-13 0.0 0.0 0.0 2.989415972748484E-4 0.0 14-15 0.0 0.0 0.0 3.736769965935605E-4 0.0 16-17 0.0 0.0 0.0 7.47353993187121E-4 0.0 18-19 0.0 0.0 0.0 8.968247918245452E-4 0.0 20-21 0.0 0.0 0.0 0.0012705017884181058 0.0 22-23 0.0 0.0 0.0 0.002578371276495567 0.0 24-25 0.0 0.0 0.0 0.00463359475776015 0.0 26-27 0.0 0.0 0.0 0.006763553638343445 0.0 28-29 0.0 0.0 0.0 0.011770825392697155 0.0 30-31 0.0 0.0 0.0 0.029595218130209988 0.0 32-33 0.0 0.0 0.0 0.05496788619891275 0.0 34-35 0.0 0.0 0.0 0.08658096011072797 0.0 36-37 0.0 0.0 0.0 0.1347479249716379 0.0 38-39 0.0 0.0 0.0 0.2168073934235838 0.0 40-41 0.0 0.0 0.0 0.31329079394404113 0.0 42-43 0.0 0.0 0.0 0.4055890121026505 0.0 44-45 0.0 0.0 0.0 0.5082007153672423 0.0 46-47 0.0 0.0 0.0 0.6325604198335792 0.0 48-49 0.0 0.0 0.0 0.776837108218353 0.0 50-51 0.0 0.0 0.0 0.9384150415454084 0.0 52-53 0.0 0.0 0.0 1.0994324593775735 0.0 54-55 0.0 0.0 0.0 1.2675123724453572 0.0 56-57 0.0 0.0 0.0 1.4595823486944473 0.0 58-59 0.0 0.0 0.0 1.6831532957563746 0.0 60-61 0.0 0.0 0.0 1.9471187261500658 0.0 62-63 0.0 0.0 0.0 2.212466761431153 0.0 64-65 0.0 0.0 0.0 2.4636524385413443 0.0 66-67 0.0 0.0 0.0 2.749889017932012 0.0 68-69 0.0 0.0 0.0 3.081452617009478 0.0 70-71 0.0 0.0 0.0 3.4647331124154928 0.0 72-73 0.0 0.0 0.0 3.8550761030571263 0.0 74-75 0.0 0.0 0.0 4.238730275459735 0.0 76-77 0.0 0.0 0.0 4.643011418074308 0.0 78-79 0.0 0.0 0.0 5.114404949277084 0.0 80-81 0.0 0.0 0.0 5.6398321741872905 0.0 82-83 0.0 0.0 0.0 6.177030224490192 0.0 84-85 0.0 0.0 0.0 6.7174418969638 0.0 86-87 0.0 0.0 0.0 7.264318181478475 0.0 88-89 0.0 0.0 0.0 7.846843251468177 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20740 0.0 30.075064 1 GGTATCA 14245 0.0 27.259052 1 TATCAAC 24765 0.0 25.098814 2 GAGTACT 12575 0.0 24.876682 12-13 ATCAACG 25045 0.0 24.685797 3 TCAACGC 25190 0.0 24.562508 4 TACATGG 14415 0.0 24.550686 2 GTACATG 14580 0.0 24.45132 1 CAACGCA 25280 0.0 24.362617 5 AACGCAG 25625 0.0 24.065287 6 AGTACTT 12675 0.0 23.652847 12-13 ACATGGG 14800 0.0 22.951714 3 CGTCACT 270 4.0017767E-11 22.855963 1 GTACTTT 13675 0.0 22.841856 14-15 ACGCAGA 27985 0.0 21.951216 7 CGCAGAG 28175 0.0 21.769564 8 TACCTGG 915 0.0 21.746323 2 CATGGGG 3565 0.0 21.52847 4 GCAGAGT 28630 0.0 21.075396 9 TAGCGCG 135 3.8640332E-4 21.055965 4 >>END_MODULE