FastQCFastQC Report
Fri 27 May 2016
SRR939207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338054
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229991.7188394489310594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173301.2951644701932807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130460.9749980195119181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78250.5848044996689222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44440.3321241145723566No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38610.2885533767695474No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38230.2857134315954364No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32100.23990063181306584No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31560.2358649202498554No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.1810838725492394No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.17368506801668693No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18680.1396057259273542No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17980.13437424797504435No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14630.1093378892032758No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14480.10821685821349512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134350.031.897251
GTATCAA195900.029.896411
GTATACC1309.835572E-625.6391161
GTACATG146700.025.6091561
TACATGG143400.025.5760962
TATCAAC244300.024.024272
ACATGGG146400.023.9176883
ATCAACG246100.023.7714333
TCAACGC248850.023.508744
GAGTACT145900.023.3022212-13
CAACGCA252200.023.2152845
AACGCAG256600.022.8541836
CATGGGG34600.022.6262054
AGTACTT147000.022.57910512-13
ATGGGAG31900.022.0127835
GTACTTT154350.021.87281214-15
CATGGGA106300.021.8709054
CGACCGT1105.7141733E-821.56657618-19
TCGACCG1005.4504017E-721.35091218-19
ATGGGGA17650.020.9678465
Produced by FastQC (version 0.11.5)