##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939207_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1338054 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.470436918091494 34.0 31.0 34.0 31.0 34.0 2 32.64161536081503 34.0 31.0 34.0 31.0 34.0 3 32.69922663808785 34.0 31.0 34.0 31.0 34.0 4 36.19788962179404 37.0 35.0 37.0 35.0 37.0 5 36.18467490848651 37.0 35.0 37.0 35.0 37.0 6 36.257166751117666 37.0 37.0 37.0 35.0 37.0 7 36.2052107015113 37.0 37.0 37.0 35.0 37.0 8 36.20679583933085 37.0 37.0 37.0 35.0 37.0 9 37.91079732208117 39.0 38.0 39.0 35.0 39.0 10-11 37.864245762876536 39.0 38.0 39.0 35.0 39.0 12-13 37.78450309180347 39.0 38.0 39.0 35.0 39.0 14-15 39.02596868287827 40.0 38.0 41.0 36.0 41.0 16-17 39.00190575268263 40.0 38.0 41.0 35.5 41.0 18-19 39.12673516913368 40.5 38.5 41.0 36.0 41.0 20-21 39.21044778461856 41.0 39.0 41.0 36.0 41.0 22-23 39.13901793201171 41.0 39.0 41.0 35.0 41.0 24-25 39.05452171586498 41.0 39.0 41.0 35.0 41.0 26-27 38.874878741814605 40.0 38.0 41.0 35.0 41.0 28-29 38.67345899343375 40.0 38.0 41.0 35.0 41.0 30-31 38.32379485431829 40.0 37.5 41.0 34.5 41.0 32-33 37.97828712443594 40.0 36.5 41.0 34.0 41.0 34-35 37.801815547055654 40.0 36.0 41.0 33.0 41.0 36-37 37.61603829142919 40.0 35.5 41.0 33.0 41.0 38-39 37.408183451490004 40.0 35.0 41.0 33.0 41.0 40-41 37.13642835042532 40.0 35.0 41.0 33.0 41.0 42-43 36.825690891399006 40.0 35.0 41.0 32.0 41.0 44-45 36.37377751570564 40.0 35.0 41.0 31.0 41.0 46-47 35.76629605382145 39.5 35.0 41.0 29.0 41.0 48-49 35.20695091528444 39.0 35.0 41.0 25.0 41.0 50-51 34.868010558617215 39.0 35.0 41.0 23.5 41.0 52-53 34.60723259300447 39.0 35.0 41.0 23.0 41.0 54-55 34.345637769477165 38.0 35.0 40.0 22.0 41.0 56-57 34.05997627898425 38.0 34.5 40.0 20.5 41.0 58-59 33.75377563237358 37.5 34.0 40.0 19.5 41.0 60-61 33.3816587372408 37.0 34.0 40.0 16.5 41.0 62-63 32.99428386298311 36.5 33.0 40.0 12.0 41.0 64-65 32.58526599075971 36.0 33.0 39.0 7.5 41.0 66-67 32.22340503447544 35.5 33.0 39.0 4.0 40.5 68-69 31.76232685676363 35.0 32.0 38.5 2.0 40.0 70-71 31.357965373594787 35.0 32.0 38.0 2.0 40.0 72-73 30.92459945562735 35.0 31.0 37.0 2.0 39.5 74-75 30.462334106097362 35.0 31.0 36.5 2.0 39.0 76-77 28.564726834641952 33.0 28.0 35.0 2.0 37.0 78-79 29.469497867799056 34.0 30.0 35.0 2.0 37.5 80-81 29.482446523085017 35.0 30.0 35.0 2.0 37.0 82-83 29.187928514095844 34.0 30.0 35.0 2.0 37.0 84-85 28.81613970736607 34.0 29.5 35.0 2.0 36.0 86-87 28.280963623291733 34.0 29.0 35.0 2.0 36.0 88-89 27.74822055014222 34.0 28.0 35.0 2.0 35.5 90-91 27.30004693383077 34.0 26.5 35.0 2.0 35.0 92-93 27.019294438042113 34.0 26.0 35.0 2.0 35.0 94-95 26.717775590521757 33.5 25.5 35.0 2.0 35.0 96-97 26.200934715639278 33.0 24.5 35.0 2.0 35.0 98-99 25.526665964153914 33.0 21.0 35.0 2.0 35.0 100-101 22.278999203320645 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16971403596260615 1101 2 0.21458921162562916 1101 3 0.2669873513158265 1101 4 0.201119760136784 1101 5 0.2306608506386283 1101 6 0.18041002467830225 1101 7 0.20051095105782224 1101 8 0.19713732090136205 1101 9 0.24928468220819155 1101 10-11 0.2603121874868961 1101 12-13 0.2653347024956716 1101 14-15 0.36558002109979526 1101 16-17 0.3637057298532156 1101 18-19 0.46226936405579266 1101 20-21 0.5320843022290518 1101 22-23 0.32321078962482375 1101 24-25 0.24992354998132527 1101 26-27 0.27428066917774885 1101 28-29 0.14991685674367972 1101 30-31 0.12762006831503925 1101 32-33 0.06783499620611622 1101 34-35 0.3051940461014411 1101 36-37 0.4082238220277645 1101 38-39 0.35152442177798804 1101 40-41 0.3364297595896346 1101 42-43 0.34284371366840105 1101 44-45 0.47895817468168644 1101 46-47 0.4237800467938442 1101 48-49 0.5577418375942216 1101 50-51 0.6717509308101342 1101 52-53 0.6502076248983215 1101 54-55 0.745124027685037 1101 56-57 0.7670192653742234 1101 58-59 0.8914748612766061 1101 60-61 0.9668884676509322 1101 62-63 0.7847064534965185 1101 64-65 0.6525495607051184 1101 66-67 0.6363795543171307 1101 68-69 0.7457073007675703 1101 70-71 0.8236426164847579 1101 72-73 0.6295615946167281 1101 74-75 0.7909859705094462 1101 76-77 0.7967341599430924 1101 78-79 1.0417385308341842 1101 80-81 0.7740461637845968 1101 82-83 0.8122496131574195 1101 84-85 0.6897214630484534 1101 86-87 0.7382711093214951 1101 88-89 0.8869302600102351 1101 90-91 0.9741859415714735 1101 92-93 0.8835645463567445 1101 94-95 0.6322060794583635 1101 96-97 0.9077922434159582 1101 98-99 0.8503895143876079 1101 100-101 0.534651511134804 1102 1 0.20267534548641208 1102 2 0.17143444972086286 1102 3 0.19109449417297242 1102 4 0.12458206172408381 1102 5 0.15620648555926664 1102 6 0.18328700880528714 1102 7 0.2208482526451192 1102 8 0.24093692407596023 1102 9 0.24358031712882422 1102 10-11 0.301346413677372 1102 12-13 0.32324640884488076 1102 14-15 0.276257104433121 1102 16-17 0.3251020592182954 1102 18-19 0.2998933719922974 1102 20-21 0.31329709984809284 1102 22-23 0.40673013486292575 1102 24-25 0.4356130341083073 1102 26-27 0.3717137048920378 1102 28-29 0.261251182140505 1102 30-31 0.37762006831503925 1102 32-33 0.38866832953944197 1102 34-35 0.4091995024506474 1102 36-37 0.40733096488490617 1102 38-39 0.5294262074922713 1102 40-41 0.5403856127642328 1102 42-43 0.5387018485890351 1102 44-45 0.42140609134835216 1102 46-47 0.3938693325081317 1102 48-49 0.2664843971180346 1102 50-51 -0.026872581094622205 1102 52-53 0.10588718839038336 1102 54-55 0.010972241970748087 1102 56-57 0.14670676537422622 1102 58-59 0.11986027794326759 1102 60-61 0.19196287241283017 1102 62-63 0.24692123524254583 1102 64-65 0.14729162419717312 1102 66-67 0.3023517765393464 1102 68-69 0.2206824991802705 1102 70-71 0.27415104902443943 1102 72-73 0.2422228049341868 1102 74-75 0.25508567289039874 1102 76-77 0.3765580686732477 1102 78-79 0.3379810903579923 1102 80-81 0.33907592568936096 1102 82-83 0.2435120139510687 1102 84-85 0.1526182884452787 1102 86-87 0.2778916450357798 1102 88-89 0.2910845258832495 1102 90-91 0.6939280050635368 1102 92-93 0.5709777408011867 1102 94-95 0.5790810794583621 1102 96-97 0.6399351005588159 1102 98-99 0.5255259231177654 1102 100-101 0.020688217484011062 1103 1 0.15880585482675258 1103 2 0.10508520862337889 1103 3 0.12547496702761407 1103 4 0.1476562249854183 1103 5 0.12955064298289187 1103 6 0.2635129768924642 1103 7 0.1345474159064537 1103 8 0.19336261487183748 1103 9 0.14172745178537127 1103 10-11 0.15326236887293732 1103 12-13 0.14180455488496335 1103 14-15 0.29315226278105655 1103 16-17 0.14341316668083692 1103 18-19 0.24734332577708074 1103 20-21 0.2927032039053117 1103 22-23 0.32815590471113865 1103 24-25 0.21071367382420902 1103 26-27 0.2865766098832765 1103 28-29 0.33885778118700927 1103 30-31 0.36303491069682536 1103 32-33 0.4188420014600496 1103 34-35 0.37680400356953925 1103 36-37 0.5249152468463762 1103 38-39 0.48732627316652355 1103 40-41 0.5468352199348914 1103 42-43 0.6422589000581951 1103 44-45 0.6560159680266864 1103 46-47 0.5126850067638173 1103 48-49 0.5759218788751781 1103 50-51 0.32956303989195135 1103 52-53 0.2877607897719727 1103 54-55 0.25291346351190214 1103 56-57 0.3951606839381512 1103 58-59 0.432718293851039 1103 60-61 0.25665657498142735 1103 62-63 0.30682695011898176 1103 64-65 0.2549396345104711 1103 66-67 0.3402816996761402 1103 68-69 0.2621196099994947 1103 70-71 0.2324288936926635 1103 72-73 0.2468526879367161 1103 74-75 0.29919916165280114 1103 76-77 0.24537298281023823 1103 78-79 0.2524426526755654 1103 80-81 0.13019530689193104 1103 82-83 0.20161351108086123 1103 84-85 0.09423813180001872 1103 86-87 0.29171744407255673 1103 88-89 0.24542647592216937 1103 90-91 0.4547885810510799 1103 92-93 0.225749309929423 1103 94-95 0.22597249802228347 1103 96-97 0.29249092367615503 1103 98-99 0.32181183112516365 1103 100-101 -0.12865534400654965 1104 1 0.06055097679954713 1104 2 0.06400599854241307 1104 3 0.11228557161405206 1104 4 0.1068526846197102 1104 5 0.03262694010471989 1104 6 0.11962960139787526 1104 7 0.12152165331852416 1104 8 0.08957960084364203 1104 9 0.11137282554633288 1104 10-11 0.13190459970431334 1104 12-13 0.07816539032636172 1104 14-15 0.042789617059391105 1104 16-17 0.10612846915095275 1104 18-19 0.09816252182394436 1104 20-21 0.06436244075718633 1104 22-23 0.16997455826359698 1104 24-25 0.08747014690291621 1104 26-27 0.11962248014456378 1104 28-29 0.0793014767532938 1104 30-31 0.09863227370225047 1104 32-33 0.11909762246874322 1104 34-35 0.19832776423515952 1104 36-37 0.18484569552463626 1104 38-39 0.4008750711286311 1104 40-41 0.6182344132692847 1104 42-43 0.6465774799021631 1104 44-45 0.696803292526802 1104 46-47 0.6650183224071213 1104 48-49 0.6466532733806574 1104 50-51 0.6266811857403951 1104 52-53 0.6351643348550269 1104 54-55 0.6747190854050871 1104 56-57 0.7836228007277626 1104 58-59 0.8850775548792953 1104 60-61 0.838874699012166 1104 62-63 0.7414630071280683 1104 64-65 0.714158206688758 1104 66-67 0.6492057327683085 1104 68-69 0.5833455884058623 1104 70-71 0.4882319623241038 1104 72-73 0.40027467782980963 1104 74-75 0.6024262663247413 1104 76-77 0.6697635610974899 1104 78-79 0.7708646130852657 1104 80-81 0.6397914139048524 1104 82-83 0.7632537016615046 1104 84-85 0.7341102918122822 1104 86-87 0.6153153613657452 1104 88-89 0.6956378718428482 1104 90-91 0.63942463806017 1104 92-93 0.6490106743028718 1104 94-95 0.48059623097351434 1104 96-97 0.3401139726126914 1104 98-99 0.3703149593130526 1104 100-101 0.01629533027862351 1105 1 0.0109504332767969 1105 2 0.037334638807841714 1105 3 0.03687042610165747 1105 4 0.04396966752275233 1105 5 0.09446902382686773 1105 6 0.05454466232590249 1105 7 0.1548478924794452 1105 8 0.13697003680781705 1105 9 0.06383232055039656 1105 10-11 0.07891386032783743 1105 12-13 0.06910872425996928 1105 14-15 0.07162742790692533 1105 16-17 0.08580502945960689 1105 18-19 0.1282561376144784 1105 20-21 0.19907463563956185 1105 22-23 0.22455875790451785 1105 24-25 0.12090279864999331 1105 26-27 0.2155206842275348 1105 28-29 0.21024693893863144 1105 30-31 0.2056437717186057 1105 32-33 0.17476368340222592 1105 34-35 0.3042273871526078 1105 36-37 0.11364680767259472 1105 38-39 0.40069089549875514 1105 40-41 0.30991826966140934 1105 42-43 0.16070375071400633 1105 44-45 0.14621911471409277 1105 46-47 -0.023016810117475472 1105 48-49 0.002629977209871015 1105 50-51 -0.05823865011137741 1105 52-53 -0.016590244978964108 1105 54-55 0.034283121225207935 1105 56-57 -0.25355407336159175 1105 58-59 0.04479738963965474 1105 60-61 0.13376375706977228 1105 62-63 0.10155569521914032 1105 64-65 0.06782725808876933 1105 66-67 -0.09326489462938525 1105 68-69 0.022843211858102563 1105 70-71 0.12123609877232511 1105 72-73 0.13990831839537776 1105 74-75 -0.05414962222035413 1105 76-77 0.0860148093989821 1105 78-79 0.2611708357658813 1105 80-81 0.05831552590546352 1105 82-83 0.09501472082123286 1105 84-85 0.0195511400570183 1105 86-87 0.023782801842912704 1105 88-89 0.14794047649553477 1105 90-91 0.35324504090002407 1105 92-93 0.5046544977343785 1105 94-95 0.3864149530402088 1105 96-97 0.3465041848375847 1105 98-99 0.3540188267284279 1105 100-101 -0.008393753972857354 1106 1 0.1192590769089179 1106 2 0.12718020343636738 1106 3 0.12162793366341873 1106 4 0.1414234453051222 1106 5 0.07180052306811291 1106 6 0.11160884178476493 1106 7 0.10363310756373068 1106 8 0.042235137097904385 1106 9 0.23361702979690335 1106 10-11 0.171461527796275 1106 12-13 0.16086472979321798 1106 14-15 0.1880720593163545 1106 16-17 0.08583664432091354 1106 18-19 0.09394304467453196 1106 20-21 0.20739708657846023 1106 22-23 0.14161103985230739 1106 24-25 0.14762316326612535 1106 26-27 0.08202919056082436 1106 28-29 0.08400750733512297 1106 30-31 0.1599648135379681 1106 32-33 0.17197512359464895 1106 34-35 0.2973005065200027 1106 36-37 0.22490660182758404 1106 38-39 0.37601259284259214 1106 40-41 0.1895888815186595 1106 42-43 0.3266585453335509 1106 44-45 0.20117155047785928 1106 46-47 0.09390914142532836 1106 48-49 0.22394691038766723 1106 50-51 0.31897568058265335 1106 52-53 0.3864792354533719 1106 54-55 0.4017465254102319 1106 56-57 0.41467053925958197 1106 58-59 0.34832199503456707 1106 60-61 0.35808216646803714 1106 62-63 0.3462140740606614 1106 64-65 0.3144780183939204 1106 66-67 0.36884372629164375 1106 68-69 0.14079374298777125 1106 70-71 0.1628657739005881 1106 72-73 0.19581500680962804 1106 74-75 0.3530736638670433 1106 76-77 0.5051878678593766 1106 78-79 0.46881143802254144 1106 80-81 0.32262157324774776 1106 82-83 0.36725416274795464 1106 84-85 0.4759219862513646 1106 86-87 0.5829025924880114 1106 88-89 0.6468739588273422 1106 90-91 0.5964846221902178 1106 92-93 0.6217811068662975 1106 94-95 0.22876260812078186 1106 96-97 0.3024550050174142 1106 98-99 0.33394274459688944 1106 100-101 0.47046816263616975 1107 1 -0.05137335122648068 1107 2 -0.032838149149192475 1107 3 0.034459286160647196 1107 4 0.050683649637115025 1107 5 0.06455640423757103 1107 6 0.12132737004480276 1107 7 0.08765188216061404 1107 8 0.029701155371974153 1107 9 0.11600958865766842 1107 10-11 0.0921283056907285 1107 12-13 0.042134174210396225 1107 14-15 0.18912586085325955 1107 16-17 0.1700444652703439 1107 18-19 0.14887444329858113 1107 20-21 0.1534096929400306 1107 22-23 0.1881506696600468 1107 24-25 0.13368084141870185 1107 26-27 0.18065654965320732 1107 28-29 0.15335415038224198 1107 30-31 0.20410793426265172 1107 32-33 0.2297379237870132 1107 34-35 0.25352664570522876 1107 36-37 0.29915973992342515 1107 38-39 0.23985812197609135 1107 40-41 0.1982934784978454 1107 42-43 0.2769703920747375 1107 44-45 0.2187886831562551 1107 46-47 0.05570484868687231 1107 48-49 0.025334149942892736 1107 50-51 -0.11094222349318983 1107 52-53 -0.04736006165241946 1107 54-55 -0.018484133221861043 1107 56-57 0.020803934095333432 1107 58-59 0.07051954540602878 1107 60-61 0.1968542116526919 1107 62-63 -0.05163035588054754 1107 64-65 0.05496742885392081 1107 66-67 0.06127843721388615 1107 68-69 -0.047182859919200126 1107 70-71 0.11385970871456053 1107 72-73 0.16295087482803794 1107 74-75 0.28589228241787623 1107 76-77 0.17415065234899174 1107 78-79 -0.015794349421152987 1107 80-81 -0.11800548160390889 1107 82-83 -0.14918281035127734 1107 84-85 -0.047933308027928945 1107 86-87 -0.059026931620614675 1107 88-89 0.15176695932786544 1107 90-91 -0.013067800449217515 1107 92-93 -0.10123899755697252 1107 94-95 0.06386536297145895 1107 96-97 0.15604161057422417 1107 98-99 0.39737938891985536 1107 100-101 0.07988318623936053 1108 1 -0.023306683775672354 1108 2 -0.014840995571752558 1108 3 -0.11591886460565348 1108 4 -0.10209043615547131 1108 5 -0.06916194107347451 1108 6 -0.030426943696838293 1108 7 -0.09687863454741574 1108 8 -0.04897772817170676 1108 9 -0.06909726980926223 1108 10-11 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-0.9313785095691323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 19.0 5 57.0 6 138.0 7 259.0 8 537.0 9 1008.0 10 1810.0 11 2861.0 12 4742.0 13 7072.0 14 7974.0 15 11240.0 16 16269.0 17 24732.0 18 22771.0 19 12098.0 20 9503.0 21 9473.0 22 9667.0 23 10098.0 24 11267.0 25 12414.0 26 14162.0 27 16277.0 28 19538.0 29 23170.0 30 27971.0 31 33401.0 32 40866.0 33 52762.0 34 74717.0 35 125861.0 36 173087.0 37 277414.0 38 256549.0 39 26262.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.03448870650102 19.423483338358 15.304532248605469 19.237495706535515 2 15.678291010676698 22.16181110777293 40.269301537905044 21.890596343645324 3 19.498615153050626 28.556620285877848 30.448995332026957 21.495769229044566 4 12.25623181127219 17.992995798375848 36.6545744790569 33.096197911295064 5 12.277680870876662 39.83067947930353 31.93974234223731 15.951897307582504 6 28.674627481402098 37.14790284995972 17.417832165219043 16.759637503419146 7 26.326889647204073 36.86831772110842 19.697037638241806 17.107754993445706 8 27.01004593237642 33.56336889243633 19.13046857600665 20.2961165991806 9 28.552584574314636 16.80163879783626 20.281991608709365 34.36378501913973 10-11 25.188445309382136 28.305471976467317 24.77721377463092 21.72886893951963 12-13 27.581248589369338 26.409845940447845 26.617647718253522 19.391257751929295 14-15 23.756418434270113 26.262209551953024 28.18390026310863 21.797471750668233 16-17 19.949829095313135 30.09013648085445 28.360200638776583 21.59983378505583 18-19 19.786010131130734 26.601243298103068 34.033006141755116 19.579740429011085 20-21 21.800988599862187 25.175665556098632 33.98977171324924 19.033574130789937 22-23 20.800206867585313 25.85116146284081 33.90793645099525 19.44069521857862 24-25 20.258286794751307 26.108751744612952 34.379571726713735 19.253389733922003 26-27 19.978236799613764 26.02701641245749 34.73491233622949 19.25983445169926 28-29 19.539196474880686 26.550311123467363 34.61822168612029 19.29227071553166 30-31 19.671745821057716 26.514740459395913 34.2786120963993 19.53490162314707 32-33 19.443572531452393 26.878362158776852 34.00322408512661 19.674841224644148 34-35 20.04841100245979 26.642457428289756 33.96386477806205 19.345266791188408 36-37 20.178883601564046 26.490083057639485 33.784996548142296 19.546036792654178 38-39 19.91738558104802 26.685496355532035 33.840553872420074 19.55656419099987 40-41 19.824738496128035 26.872234677605313 33.7098102118528 19.59321661441385 42-43 19.778470309797 27.22085588536573 32.90465941837525 20.096014386462016 44-45 20.015762149242665 27.20603759821915 32.5831800902157 20.195020162322486 46-47 20.29346733067446 27.718227417562975 31.643643502998607 20.344661748763954 48-49 20.355294478137882 27.870886255693943 31.12985736653102 20.643961899637155 50-51 20.203982951347964 28.41273850886325 30.770866325922814 20.61241221386597 52-53 20.578437259795663 28.686147452250598 30.095694977816656 20.639720310137076 54-55 19.901663161576437 28.47067457666133 30.282036449948958 21.345625811813278 56-57 20.3715993524925 28.653141054097965 29.772677335892272 21.20258225751726 58-59 20.607196855607732 28.19519566876258 29.996165922148037 21.201441553481647 60-61 20.43674477043114 28.40328390091792 30.525419662296954 20.634551666353993 62-63 20.541531530420556 28.44753910963478 30.33565358484277 20.675275775101895 64-65 20.259104706507063 28.5759181124795 30.385891801420073 20.779085379593365 66-67 20.322210691801573 28.655578280075623 29.91177546596373 21.110435562159076 68-69 20.397792243705204 28.605055268645224 29.991741466805184 21.005411020844388 70-71 20.44387612303992 28.66940671654758 29.725866716091698 21.1608504443208 72-73 20.311586826839577 28.58879387528456 29.772490497393978 21.327128800481894 74-75 19.885781889221214 29.246839066285812 29.489504907873677 21.377874136619297 76-77 19.882664253941336 28.70789870448334 29.92492189975156 21.48451514182376 78-79 19.875161335887302 28.763339404370697 29.92581725455085 21.435682005191147 80-81 19.847280320656022 29.15664865729402 29.596668776770223 21.399402245279735 82-83 19.839698903885484 28.84205271199874 29.483847510303285 21.83440087381249 84-85 19.760114315266797 28.574855723311614 29.427287687940844 22.23774227348074 86-87 19.750388778343783 28.50731935788819 29.412779163800163 22.329512699967864 88-89 19.775779470077413 28.437300198413297 29.273840362847185 22.513079968662105 90-91 19.723262059169784 28.305424684916275 29.47226805508347 22.49904520083047 92-93 19.74841822527342 28.38002053728773 29.391003651571612 22.480557585867235 94-95 19.589044986226266 28.15189073086736 29.44978304313578 22.80928123977059 96-97 19.48486383957598 27.952758259382655 29.588006164175734 22.974371736865628 98-99 19.665016486850877 28.01041974954336 29.108783321226035 23.215780442379728 100-101 19.838525368766174 27.329951279592656 29.806216990036944 23.025306361604223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6295.0 1 4174.0 2 2825.0 3 3577.5 4 2941.0 5 2703.0 6 4729.5 7 9502.0 8 13912.5 9 14076.0 10 10337.0 11 7034.5 12 6263.0 13 6172.5 14 6037.0 15 5788.0 16 5573.0 17 5488.5 18 5521.5 19 5511.0 20 5670.5 21 6127.5 22 6613.0 23 7306.0 24 8204.0 25 9117.0 26 10411.0 27 11734.5 28 13220.0 29 14974.5 30 16675.0 31 18457.0 32 20826.5 33 22706.0 34 23985.0 35 25275.0 36 27502.0 37 30520.5 38 32270.0 39 32658.0 40 32737.0 41 34487.5 42 36541.0 43 38066.5 44 39635.0 45 41166.5 46 41982.5 47 45105.0 48 54871.5 49 54647.5 50 46001.0 51 41636.5 52 38148.0 53 37532.0 54 37029.0 55 34535.0 56 31338.5 57 28155.0 58 26119.0 59 24068.5 60 20902.5 61 18167.0 62 15659.5 63 11798.5 64 8562.0 65 6892.5 66 5270.5 67 4361.0 68 3352.5 69 2223.5 70 1690.5 71 1307.0 72 1054.5 73 954.5 74 763.0 75 580.5 76 422.0 77 251.5 78 157.5 79 113.0 80 83.5 81 47.5 82 25.0 83 15.0 84 7.0 85 7.0 86 6.0 87 2.0 88 3.5 89 3.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34617436964427445 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010089278908026133 16-17 0.002055223481264583 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.8578009557162863E-4 26-27 0.0011583986894400375 28-29 0.0 30-31 0.0026904743754736357 32-33 0.0 34-35 0.010089278908026134 36-37 0.02753999464894541 38-39 0.020402764014008402 40-41 0.012518179385884277 42-43 0.009678234211773218 44-45 0.0028773128737704158 46-47 0.0013078694880774618 48-49 6.726185938684089E-4 50-51 7.47353993187121E-5 52-53 3.736769965935605E-5 54-55 0.0 56-57 0.0 58-59 0.003960976163891741 60-61 0.00993980810938871 62-63 0.0039236084642323855 64-65 0.0017562818839897344 66-67 0.0035499314676388245 68-69 0.0032883575700233324 70-71 3.736769965935605E-5 72-73 0.0 74-75 0.0 76-77 0.21755474741677094 78-79 5.231477952309846E-4 80-81 0.003512563767979469 82-83 7.099862935277649E-4 84-85 0.0 86-87 0.11176678968113395 88-89 0.4096994590651797 90-91 0.3970691765803174 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0013452371877368178 100-101 8.220893925058331E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1338054.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.11211527083632 #Duplication Level Percentage of deduplicated Percentage of total 1 68.23668462432909 28.735911285994515 2 14.878122371606601 12.530984086534117 3 6.3239736401107525 7.989477207062233 4 3.3432982970375758 5.631734530785487 5 1.9511536580030506 4.108360387846921 6 1.246988566694995 3.1507995757244758 7 0.8500177432783979 2.505723163103723 8 0.5807849633572241 1.9566466659574295 9 0.4304682557814669 1.631513592711449 >10 1.9681725686810851 14.241791496003984 >50 0.11243369524620353 3.2403983653322754 >100 0.06902900386658282 5.484730779831694 >500 0.005678471684490064 1.7387171282693803 >1k 0.0024843313619644027 2.479803549425844 >5k 1.774522401403145E-4 0.5847535792628142 >10k+ 5.323567204209435E-4 3.9886546061537014 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22999 1.7188394489310594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17330 1.2951644701932807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13046 0.9749980195119181 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7825 0.5848044996689222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4444 0.3321241145723566 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3861 0.2885533767695474 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3823 0.2857134315954364 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3210 0.23990063181306584 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3156 0.2358649202498554 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2423 0.1810838725492394 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2324 0.17368506801668693 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1868 0.1396057259273542 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1798 0.13437424797504435 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1463 0.1093378892032758 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1448 0.10821685821349512 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.47353993187121E-5 0.0 12-13 0.0 0.0 0.0 1.494707986374242E-4 0.0 14-15 0.0 0.0 0.0 2.242061979561363E-4 0.0 16-17 0.0 0.0 0.0 5.978831945496968E-4 0.0 18-19 0.0 0.0 0.0 7.47353993187121E-4 0.0 20-21 0.0 0.0 0.0 0.0011210309897806815 0.0 22-23 0.0 0.0 0.0 0.002428900477858143 0.0 24-25 0.0 0.0 0.0 0.004409388559804014 0.0 26-27 0.0 0.0 0.0 0.006576715140046666 0.0 28-29 0.0 0.0 0.0 0.011808193092356511 0.0 30-31 0.0 0.0 0.0 0.02970732122918806 0.0 32-33 0.0 0.0 0.0 0.05552840169380309 0.0 34-35 0.0 0.0 0.0 0.08747778490255251 0.0 36-37 0.0 0.0 0.0 0.1361305298590341 0.0 38-39 0.0 0.0 0.0 0.21916155850212324 0.0 40-41 0.0 0.0 0.0 0.3167286223127019 0.0 42-43 0.0 0.0 0.0 0.4099984006624546 0.0 44-45 0.0 0.0 0.0 0.5143290181113767 0.0 46-47 0.0 0.0 0.0 0.6404076367620439 0.0 48-49 0.0 0.0 0.0 0.7862164008328514 0.0 50-51 0.0 0.0 0.0 0.9497374545421934 0.0 52-53 0.0 0.0 0.0 1.112286948060392 0.0 54-55 0.0 0.0 0.0 1.2812636859200002 0.0 56-57 0.0 0.0 0.0 1.4759494011452452 0.0 58-59 0.0 0.0 0.0 1.702173454882987 0.0 60-61 0.0 0.0 0.0 1.967297283966118 0.0 62-63 0.0 0.0 0.0 2.2362326184145034 0.0 64-65 0.0 0.0 0.0 2.489324048207322 0.0 66-67 0.0 0.0 0.0 2.7770927032840227 0.0 68-69 0.0 0.0 0.0 3.112430440027084 0.0 70-71 0.0 0.0 0.0 3.4992982346003974 0.0 72-73 0.0 0.0 0.0 3.891509610224998 0.0 74-75 0.0 0.0 0.0 4.2758737689211355 0.0 76-77 0.0 0.0 0.0 4.683742210703006 0.0 78-79 0.0 0.0 0.0 5.157714113182278 0.0 80-81 0.0 0.0 0.0 5.685458135471364 0.0 82-83 0.0 0.0 0.0 6.229008694716357 0.0 84-85 0.0 0.0 0.0 6.773418711053515 0.0 86-87 0.0 0.0 0.0 7.322537057547752 0.0 88-89 0.0 0.0 0.0 7.907715234213268 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13435 0.0 31.89725 1 GTATCAA 19590 0.0 29.89641 1 GTATACC 130 9.835572E-6 25.639116 1 GTACATG 14670 0.0 25.609156 1 TACATGG 14340 0.0 25.576096 2 TATCAAC 24430 0.0 24.02427 2 ACATGGG 14640 0.0 23.917688 3 ATCAACG 24610 0.0 23.771433 3 TCAACGC 24885 0.0 23.50874 4 GAGTACT 14590 0.0 23.30222 12-13 CAACGCA 25220 0.0 23.215284 5 AACGCAG 25660 0.0 22.854183 6 CATGGGG 3460 0.0 22.626205 4 AGTACTT 14700 0.0 22.579105 12-13 ATGGGAG 3190 0.0 22.012783 5 GTACTTT 15435 0.0 21.872812 14-15 CATGGGA 10630 0.0 21.870905 4 CGACCGT 110 5.7141733E-8 21.566576 18-19 TCGACCG 100 5.4504017E-7 21.350912 18-19 ATGGGGA 1765 0.0 20.967846 5 >>END_MODULE