FastQCFastQC Report
Fri 27 May 2016
SRR939206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316456
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226361.719464987815772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160461.2188785648741773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118670.9014353688995302No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77700.5902210176413036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40450.3072643521697649No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35730.27141051428988133No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.25583840249883016No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31800.2415576365636223No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.2322903310099236No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.18040861221339718No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.15784804049660603No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19550.14850477342197535No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18370.13954131395200448No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15210.11553747333750615No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14120.10725766755592288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGG2050.032.3410153
GGTATCA126750.031.992411
GTATCAA190050.031.7052231
ATCGCGT653.1261698E-729.13691124-25
TATCAAC233550.025.7109932
ATCAACG236050.025.4386863
GTACATG145800.025.2720951
TCAACGC238750.025.1906744
TACATGG143650.024.9556982
GTCGGGA8750.024.8959832
CAACGCA241250.024.8894255
ATGGGAG33050.024.7877775
AACGCAG245100.024.5056726
GAGTACT132150.024.35649712-13
TCGCGAT601.6977143E-423.77861250-51
CGCGTCA701.825752E-523.67329226-27
AGTACTT132700.023.6491612-13
ACATGGG146400.023.6458973
CATGGGG34800.023.4060784
GTACTTT139400.022.86947414-15
Produced by FastQC (version 0.11.5)