FastQCFastQC Report
Fri 27 May 2016
SRR939206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316456
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223531.6979678773920284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166931.2680256689171534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129270.9819545810874044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76780.5832325577155636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.3226845409189521No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37000.2810576274482398No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.27642397467139046No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30900.23472109967974625No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30320.23031533146569272No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22860.17364803685045305No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.16673553844564495No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19580.14873265798477123No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16680.12670381691450378No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14280.10847305189083417No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13520.10269997630000546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141750.030.121811
GTATCAA204500.029.454381
CGACCGT1200.027.68287818-19
TACGCTA1056.949933E-527.1179229
TCGACCG1209.094947E-1225.7055318-19
GATAGTC2800.025.423057
GTAAGCG851.13401256E-725.12395594-95
TATCAAC251650.023.874332
TACATGG138650.023.6853962
ATCAACG254200.023.560163
GTACATG142650.023.484411
TCAACGC255650.023.4450954
GAGTACT142400.023.3624812-13
AGTACTT143200.023.29824612-13
CAACGCA258400.023.1955815
AACGCAG261900.022.88566
ACATGGG141350.022.2593333
GTTCGAC1406.730261E-1122.03414716-17
GTACTTT150800.021.9352414-15
ACCGTCC1100.002892974321.5710728
Produced by FastQC (version 0.11.5)