##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939205_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 981997 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.257681031612115 33.0 31.0 34.0 31.0 34.0 2 32.24131336450111 34.0 31.0 34.0 30.0 34.0 3 32.31671787184686 34.0 31.0 34.0 30.0 34.0 4 35.706830061598964 37.0 35.0 37.0 35.0 37.0 5 35.80476111434149 37.0 35.0 37.0 35.0 37.0 6 35.97957936735041 37.0 37.0 37.0 35.0 37.0 7 35.950005957248344 37.0 37.0 37.0 35.0 37.0 8 35.97193983281008 37.0 37.0 37.0 35.0 37.0 9 37.59700182383449 39.0 38.0 39.0 35.0 39.0 10-11 37.508810108381184 39.0 37.5 39.0 35.0 39.0 12-13 37.45905741056235 39.0 37.5 39.0 35.0 39.0 14-15 38.52911312356351 40.0 38.0 41.0 34.0 41.0 16-17 38.58017794351714 40.0 38.0 41.0 34.5 41.0 18-19 38.6891141215299 40.0 38.0 41.0 35.0 41.0 20-21 38.6002223021048 40.0 38.0 41.0 34.5 41.0 22-23 38.491762194792855 40.0 38.0 41.0 34.5 41.0 24-25 38.45999224030216 40.0 38.0 41.0 34.5 41.0 26-27 38.23732608144424 40.0 37.5 41.0 34.0 41.0 28-29 38.120289064019545 40.0 38.0 41.0 34.0 41.0 30-31 37.95660781041083 40.0 37.0 41.0 34.0 41.0 32-33 37.617516143124675 40.0 35.5 41.0 33.5 41.0 34-35 37.15427542039335 40.0 35.0 41.0 33.0 41.0 36-37 36.77989087543037 39.0 35.0 41.0 32.0 41.0 38-39 36.45956963208645 39.0 35.0 40.5 31.0 41.0 40-41 36.392005270891865 39.0 35.0 41.0 31.0 41.0 42-43 36.196905896861196 39.0 35.0 41.0 30.5 41.0 44-45 35.83748066440121 39.0 35.0 41.0 30.0 41.0 46-47 35.280928047641694 38.0 35.0 40.0 27.5 41.0 48-49 35.00528667602855 38.5 35.0 41.0 24.5 41.0 50-51 33.8317148626727 38.0 34.0 40.0 23.0 40.5 52-53 34.050083656060046 37.5 34.0 39.5 23.0 40.5 54-55 34.60664849281616 38.0 35.0 40.0 23.0 41.0 56-57 34.404953375621304 38.0 35.0 40.5 22.5 41.0 58-59 34.243535876382516 38.0 35.0 40.0 22.0 41.0 60-61 33.75415098009464 37.5 34.0 40.0 21.0 41.0 62-63 33.4018489873187 37.0 34.0 40.0 19.5 41.0 64-65 32.97073921814425 36.0 33.0 39.5 15.5 41.0 66-67 32.402591861278594 36.0 33.0 39.0 10.0 40.0 68-69 31.90768403569461 35.0 32.5 38.0 6.5 39.5 70-71 31.301451022762798 35.0 31.5 37.0 2.0 39.0 72-73 30.758578183029073 35.0 31.5 37.0 2.0 39.0 74-75 30.363317301376682 35.0 31.0 36.0 2.0 37.5 76-77 29.91310105835354 35.0 31.0 35.5 2.0 37.0 78-79 29.46758187652304 34.0 30.0 35.0 2.0 36.5 80-81 28.94458231542459 34.0 29.0 35.0 2.0 36.0 82-83 28.542831597245204 34.0 29.0 35.0 2.0 36.0 84-85 28.18276736079642 34.0 29.0 35.0 2.0 35.0 86-87 27.926002319762688 34.0 29.0 35.0 2.0 35.0 88-89 27.42105882197196 34.0 26.5 35.0 2.0 35.0 90-91 26.902935039516414 33.5 25.0 35.0 2.0 35.0 92-93 26.480148106358776 33.0 25.0 35.0 2.0 35.0 94-95 26.023252616861356 33.0 24.0 35.0 2.0 35.0 96-97 25.390054144768264 33.0 19.5 35.0 2.0 35.0 98-99 24.728705383010336 33.0 6.0 35.0 2.0 35.0 100-101 22.281646481608398 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.061105241761922 1101 2 0.12533066517141833 1101 3 0.14121109573973456 1101 4 0.016508934237265294 1101 5 0.15250142062205185 1101 6 0.1880434389941925 1101 7 -0.011424870011239818 1101 8 0.1362427539117803 1101 9 0.359341708570895 1101 10-11 0.26909009410888984 1101 12-13 0.23083771371143058 1101 14-15 0.24613261500814332 1101 16-17 0.4206983235373585 1101 18-19 0.37348870050042393 1101 20-21 0.3329209520538896 1101 22-23 0.4012195330364321 1101 24-25 0.433538412889952 1101 26-27 0.39879549966145333 1101 28-29 0.5176748594336047 1101 30-31 0.4721417945942008 1101 32-33 0.35207178263727457 1101 34-35 0.3634975139814429 1101 36-37 0.2689133104568029 1101 38-39 0.3873663396526581 1101 40-41 0.14779578218260525 1101 42-43 -0.050332513460404016 1101 44-45 0.2198594225443813 1101 46-47 0.3642789090199443 1101 48-49 0.499294134130281 1101 50-51 0.5561553586285655 1101 52-53 0.4384555441828155 1101 54-55 0.5747187418609911 1101 56-57 0.6070872987268032 1101 58-59 0.6061133823225688 1101 60-61 0.6542060625746728 1101 62-63 0.7490612850150882 1101 64-65 0.4656133373005602 1101 66-67 0.4560284231396423 1101 68-69 0.39313224512755696 1101 70-71 0.41089932206181246 1101 72-73 0.2881417570047162 1101 74-75 0.1566361089445678 1101 76-77 0.1001560848252332 1101 78-79 0.2708657914503547 1101 80-81 0.2898079100514259 1101 82-83 0.37432759824947226 1101 84-85 0.21097260607318447 1101 86-87 0.1902755672633809 1101 88-89 0.07482543707914502 1101 90-91 0.011558420686220927 1101 92-93 -0.05936725358837336 1101 94-95 -0.5429690190219816 1101 96-97 -0.6492122303878176 1101 98-99 -0.7570355476447865 1101 100-101 -0.602544879240682 1102 1 0.014371065414295003 1102 2 0.033498651189717066 1102 3 0.2560889272054183 1102 4 0.19733721291174078 1102 5 0.10084109369699945 1102 6 0.11828057959937865 1102 7 0.09940066715335405 1102 8 0.003022507861267343 1102 9 0.290461272670818 1102 10-11 0.06510462411128515 1102 12-13 0.12272370116902209 1102 14-15 0.17275867305961157 1102 16-17 0.24550299095794514 1102 18-19 0.20954212567433927 1102 20-21 0.22713293930586786 1102 22-23 0.5230839311036704 1102 24-25 0.3801346570214079 1102 26-27 0.3679406626099464 1102 28-29 0.34741424465001103 1102 30-31 0.2349112336127419 1102 32-33 0.44560867307968266 1102 34-35 0.4680518472805133 1102 36-37 0.4503412944189691 1102 38-39 0.38161345250006207 1102 40-41 0.38729237242967685 1102 42-43 0.45114533171283 1102 44-45 0.6287213532527218 1102 46-47 0.8092687997022381 1102 48-49 0.769461366233358 1102 50-51 0.7404662115808307 1102 52-53 0.8078140308693591 1102 54-55 0.717482869204126 1102 56-57 0.5437198678642616 1102 58-59 0.5822451118726164 1102 60-61 0.48781354689666756 1102 62-63 0.36614945882681127 1102 64-65 0.4128606900300795 1102 66-67 0.5549840792172276 1102 68-69 0.2950718633723035 1102 70-71 0.43666523138637103 1102 72-73 0.26259431174923975 1102 74-75 0.07730366660194576 1102 76-77 -0.2320677937258786 1102 78-79 -0.2644611336036178 1102 80-81 -0.11799168557586626 1102 82-83 -0.4701559698256439 1102 84-85 -0.2398130078536873 1102 86-87 -0.4899058864222319 1102 88-89 -0.8087203632703996 1102 90-91 -0.6661299724462957 1102 92-93 -0.6821911801159111 1102 94-95 -0.42475425599124605 1102 96-97 -0.39010099362416994 1102 98-99 -0.5607305889387781 1102 100-101 -0.6012983489641321 1103 1 0.30079293383865746 1103 2 0.3245579678971069 1103 3 0.4387434496056315 1103 4 0.3540498988059184 1103 5 0.28313626167810924 1103 6 0.26638242310121285 1103 7 0.23159663691838972 1103 8 0.29652078897207446 1103 9 0.42409192737904533 1103 10-11 0.43050111637491284 1103 12-13 0.39260775129827863 1103 14-15 0.44389978047242806 1103 16-17 0.4737306221701303 1103 18-19 0.5099166467628393 1103 20-21 0.45828527825223375 1103 22-23 0.6258859391160385 1103 24-25 0.6874188940696158 1103 26-27 0.6056768390789316 1103 28-29 0.5326614476975564 1103 30-31 0.6232688336171179 1103 32-33 0.6179102356565451 1103 34-35 0.37919292736599175 1103 36-37 0.42519331691482165 1103 38-39 0.44693739069656147 1103 40-41 0.49742288776851495 1103 42-43 0.4838773270999681 1103 44-45 0.32007143277525785 1103 46-47 0.061285556822376464 1103 48-49 -0.13781289633858762 1103 50-51 0.00723084673361285 1103 52-53 -0.045414966709444116 1103 54-55 -0.07559183825677707 1103 56-57 -0.11045615841370449 1103 58-59 -0.23687937505672352 1103 60-61 -0.32982023405836713 1103 62-63 -0.25339942157238937 1103 64-65 -0.1977733314054788 1103 66-67 -0.3128315651339406 1103 68-69 -0.3460123212074322 1103 70-71 -0.44223437892587114 1103 72-73 -0.26243479185960794 1103 74-75 -0.21427486941820817 1103 76-77 -0.10015534503294177 1103 78-79 -0.19741693984190078 1103 80-81 -0.09573630619051343 1103 82-83 0.029837881524599652 1103 84-85 0.04302283139601215 1103 86-87 -0.012810973131859527 1103 88-89 -0.0959893789945454 1103 90-91 -0.4212490649602465 1103 92-93 -0.5210630805132475 1103 94-95 -0.35871980715620566 1103 96-97 -0.6073526018376754 1103 98-99 -0.7080515539215355 1103 100-101 -0.5528244438336998 1104 1 0.31412986284721 1104 2 0.34123113169969344 1104 3 0.3791237723619787 1104 4 0.1523378589900517 1104 5 0.321816025858773 1104 6 0.34164061750264096 1104 7 0.2768460735366034 1104 8 0.32564709329435715 1104 9 0.5052015491923356 1104 10-11 0.43347155351646194 1104 12-13 0.44449761683426203 1104 14-15 0.49897168507666834 1104 16-17 0.4490499608506937 1104 18-19 0.6865955778860737 1104 20-21 0.6744129496431199 1104 22-23 0.6050201201031058 1104 24-25 0.6212295443884202 1104 26-27 0.723167974493478 1104 28-29 0.680973705389988 1104 30-31 0.5915599177665953 1104 32-33 0.688259532363837 1104 34-35 0.5479017417543872 1104 36-37 0.6538075045236269 1104 38-39 0.5830822038460539 1104 40-41 0.6780922173708817 1104 42-43 0.74354960444402 1104 44-45 0.8529832087896096 1104 46-47 0.8356691744276574 1104 48-49 1.005950253686514 1104 50-51 1.095973490729726 1104 52-53 1.0200561379460709 1104 54-55 1.019166441586428 1104 56-57 1.0134537912824513 1104 58-59 1.0104792721861813 1104 60-61 1.1175729904078864 1104 62-63 1.045810375396563 1104 64-65 1.2290971762255367 1104 66-67 1.1645724196574179 1104 68-69 1.0646776479267182 1104 70-71 1.2649753059007374 1104 72-73 1.2488328438593328 1104 74-75 1.169649792285714 1104 76-77 1.2491340196005645 1104 78-79 1.3025964104373848 1104 80-81 1.2858987826155435 1104 82-83 1.254679280206659 1104 84-85 1.2800296720791202 1104 86-87 1.3471666250994971 1104 88-89 1.4273081933906688 1104 90-91 1.5125322147441267 1104 92-93 1.626898064833913 1104 94-95 1.2091697005922946 1104 96-97 1.1788894013450317 1104 98-99 1.493828031899401 1104 100-101 1.1104941952922296 1105 1 0.049990747649680145 1105 2 0.10519023501392155 1105 3 0.14900312663544213 1105 4 0.10973420739234996 1105 5 0.11180258161462575 1105 6 0.16923706862500865 1105 7 0.14688756438746964 1105 8 0.11056310453314211 1105 9 0.07712196128107962 1105 10-11 0.19873479886636858 1105 12-13 0.2032464461276149 1105 14-15 0.17341767647638306 1105 16-17 0.12127601549374134 1105 18-19 0.16222851477365907 1105 20-21 0.11674117047112276 1105 22-23 0.16729101406981783 1105 24-25 0.09700346960418216 1105 26-27 0.3211643037616483 1105 28-29 0.3650578854925044 1105 30-31 0.24994205152423632 1105 32-33 0.37683268012967375 1105 34-35 0.2825478610506593 1105 36-37 0.3861222811074825 1105 38-39 0.5269298256750758 1105 40-41 0.32582532646564033 1105 42-43 0.12025153497705077 1105 44-45 0.10469961580277243 1105 46-47 0.10469945167113082 1105 48-49 0.026781908587366843 1105 50-51 0.05337172736856388 1105 52-53 0.16135036044579465 1105 54-55 0.20433887534709783 1105 56-57 0.10655581144377635 1105 58-59 0.35838622492487815 1105 60-61 0.27282253974876625 1105 62-63 0.18638517977525737 1105 64-65 0.324097503519873 1105 66-67 0.5474155248246433 1105 68-69 0.41851743986823564 1105 70-71 0.4894312420128806 1105 72-73 0.45469090889523045 1105 74-75 0.5624860746249105 1105 76-77 0.6921841761983138 1105 78-79 0.48924229810997133 1105 80-81 0.45491483514376796 1105 82-83 0.46040516868489334 1105 84-85 0.4054595605976381 1105 86-87 0.3939967794817143 1105 88-89 0.4674155962849582 1105 90-91 0.8334480849945791 1105 92-93 1.0520307449213249 1105 94-95 0.7195443015449712 1105 96-97 0.7317386243986377 1105 98-99 0.4924489898143847 1105 100-101 0.32385548291849275 1106 1 0.24367996100033196 1106 2 0.21897541277817112 1106 3 0.29704573061685835 1106 4 0.2668064299607664 1106 5 0.16351407481514713 1106 6 0.15852495707236613 1106 7 0.15174090948067942 1106 8 0.137358362874906 1106 9 0.19462204516605652 1106 10-11 0.11946169814207508 1106 12-13 0.16735026696847655 1106 14-15 0.1825455603220547 1106 16-17 0.37495437173166835 1106 18-19 0.36212941066521154 1106 20-21 0.3954627873900023 1106 22-23 0.29517426056222007 1106 24-25 0.3509328815944599 1106 26-27 0.3022488406446726 1106 28-29 0.2313272569303848 1106 30-31 0.20237028193469797 1106 32-33 0.3899241291347906 1106 34-35 0.4417561546384903 1106 36-37 0.34537635192558014 1106 38-39 0.4009130199191304 1106 40-41 0.42238831928987963 1106 42-43 0.45925109877517656 1106 44-45 0.5106867262768944 1106 46-47 0.3952596355469993 1106 48-49 0.44751659718237136 1106 50-51 0.17748314579162638 1106 52-53 -0.07172704381747508 1106 54-55 -0.15433171553868164 1106 56-57 -0.09476753398497095 1106 58-59 0.18382218713349374 1106 60-61 0.044233580929102345 1106 62-63 0.08670166580960625 1106 64-65 -0.006542922328200973 1106 66-67 -0.17154943042871196 1106 68-69 -0.12566178158331454 1106 70-71 -0.050762244678168145 1106 72-73 0.009865054107319082 1106 74-75 -0.010706563629515387 1106 76-77 -0.3143324881515319 1106 78-79 -0.33256444049006006 1106 80-81 -0.22365111522508485 1106 82-83 0.15689673944307003 1106 84-85 -0.03766342915880827 1106 86-87 -0.05871905656389842 1106 88-89 0.0717535281128363 1106 90-91 0.007930035344259068 1106 92-93 0.09606496519847951 1106 94-95 -0.05532040397082483 1106 96-97 0.13709420083223023 1106 98-99 0.03953182982416337 1106 100-101 -0.10622638881898183 1107 1 0.16257691437642308 1107 2 0.16668548780249637 1107 3 0.14136910528196722 1107 4 0.23995883493532943 1107 5 0.13677369033278097 1107 6 0.17995669047087404 1107 7 0.18491162094367297 1107 8 0.10287576326132353 1107 9 0.2743094515009332 1107 10-11 0.2530938760608592 1107 12-13 0.16328606514520771 1107 14-15 0.3321296295270386 1107 16-17 0.33337685505063774 1107 18-19 0.3511264963829248 1107 20-21 0.23440200247049603 1107 22-23 0.3814574208984709 1107 24-25 0.37695622352000413 1107 26-27 0.35828018301290143 1107 28-29 0.20615583534164728 1107 30-31 0.2950895332592651 1107 32-33 0.1629369298430774 1107 34-35 0.2777015441240138 1107 36-37 0.4791238099981854 1107 38-39 0.6288241896437938 1107 40-41 0.47519412295394403 1107 42-43 0.4992060472910609 1107 44-45 0.5421507150624407 1107 46-47 0.9085035960197416 1107 48-49 0.7899597525385147 1107 50-51 0.4941738675837186 1107 52-53 0.7482647251795385 1107 54-55 0.7528032037421539 1107 56-57 0.6330946416743117 1107 58-59 0.7127845965809385 1107 60-61 0.5176061709974817 1107 62-63 0.6998670052174418 1107 64-65 0.5729467785428497 1107 66-67 0.49417616591552616 1107 68-69 0.6813782621309237 1107 70-71 0.647314997636105 1107 72-73 0.887198324420833 1107 74-75 0.759719350426824 1107 76-77 0.6315942028930479 1107 78-79 0.5685666883793203 1107 80-81 0.5706717983194096 1107 82-83 0.5817369611498648 1107 84-85 0.41191706636140424 1107 86-87 0.3225591006136739 1107 88-89 0.3751553301721984 1107 90-91 0.5246237544203609 1107 92-93 0.572426193254902 1107 94-95 0.282176079984481 1107 96-97 0.5712329197469401 1107 98-99 0.6430516787610507 1107 100-101 0.410575693601718 1108 1 0.023273720626342254 1108 2 0.19876758150744678 1108 3 0.16314676524332583 1108 4 0.15362264333661813 1108 5 0.15837718159367142 1108 6 0.19453756828112034 1108 7 0.19369673401092768 1108 8 0.2528569223704551 1108 9 0.1946311049424594 1108 10-11 0.30422224825170474 1108 12-13 0.2703236855845077 1108 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-0.7896620094914297 2316 98-99 -0.6826183245776072 2316 100-101 -0.6515360179385574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2990.0 3 531.0 4 466.0 5 530.0 6 676.0 7 762.0 8 1056.0 9 1356.0 10 1799.0 11 2579.0 12 4122.0 13 7003.0 14 6308.0 15 6860.0 16 9210.0 17 13306.0 18 12454.0 19 6904.0 20 6669.0 21 7130.0 22 7712.0 23 8369.0 24 8994.0 25 10072.0 26 11473.0 27 13134.0 28 15524.0 29 18199.0 30 21784.0 31 26423.0 32 33171.0 33 43335.0 34 62921.0 35 114594.0 36 148151.0 37 194899.0 38 133863.0 39 16656.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.78470139760897 16.792968123399092 13.131128396320587 16.291202082671344 2 13.390037170476488 26.083255342618816 36.283421315015204 24.243286171889498 3 28.923164751817154 28.526092585607536 28.531903397795972 14.018839264779343 4 11.045319043948828 15.563867921065832 41.52485349710665 31.865959537878695 5 10.41874854693255 43.38359757067516 32.20109637763031 13.996557504761983 6 24.22912831927857 37.15828782606746 17.921403397196386 20.691180457457587 7 24.269951532263406 38.16892866425772 20.75458812616901 16.806531677309863 8 23.008265911803772 25.623511111428243 20.33277710783809 31.035445868929894 9 24.112759547135347 23.789153753011828 24.94251538019349 27.155571319659334 10-11 24.84270992479226 24.443025473195203 25.39390041675627 25.320364185256267 12-13 34.57837379447277 23.71685628260351 23.721290054285674 17.983479868638046 14-15 26.764465305436 25.06881324231282 32.295363101016974 15.871358351234205 16-17 17.855155474474874 29.12432862136793 32.46506792772042 20.555447976436774 18-19 16.91425979440291 33.81221044906197 31.883603708290646 17.389926048244476 20-21 21.12806355902182 26.84264636246727 28.460930336160022 23.568359742350882 22-23 18.148540470442384 27.30225597024664 29.495883221323243 25.053320337987735 24-25 23.32112637137137 27.016098812663497 29.807504554031226 19.855270261933907 26-27 23.290141413770638 22.166295008152016 36.444354716188506 18.09920886188884 28-29 17.58621304927443 30.391702543632253 33.84154998687273 18.18053442022059 30-31 18.59561446837013 28.613817427026323 31.350480102397377 21.440088002206174 32-33 19.19284490619567 28.70284616517738 31.68342165744178 20.42088727118517 34-35 22.761106158864113 25.404567362336756 31.295550384785336 20.538776094013798 36-37 22.272125383483452 24.365551059836722 35.419033058883 17.943290497796824 38-39 21.887898512730665 26.254869112625805 34.632121436883764 17.225110937759762 40-41 18.961504413238025 29.271640566706914 33.70063011189204 18.06622490816302 42-43 16.927686559223396 31.35732731454577 29.126320872860617 22.588665253370223 44-45 19.54825978873632 27.641499443113975 28.69444121846764 24.11579954968206 46-47 22.280015431230233 29.445969716722313 28.149562184890286 20.124452667157165 48-49 21.982635767126673 28.012477440159817 29.57261799557579 20.43226879713772 50-51 18.2767992730458 29.632695747563513 33.91322188885296 18.177283090537724 52-53 19.234598802922186 32.17057079674544 28.995162774134197 19.599667626198176 54-55 15.988869709709997 28.314044445444896 31.65088050848585 24.046205336359254 56-57 21.324569295554063 27.953502384263608 28.344170740177923 22.37775758000441 58-59 24.45485861098383 26.76976623355371 27.74621367087779 21.02916148458467 60-61 20.540424487792908 27.050208563366457 33.59623563570932 18.81313131313131 62-63 21.10746043121674 27.37963882311356 31.7998431622902 19.713057583379502 64-65 19.126989974906564 32.351035871741026 30.342332993569038 18.17964115978337 66-67 19.511869354201508 28.13779995783242 29.03329805722099 23.31703263074508 68-69 17.36773900332879 30.541890326041116 29.62017827238743 22.47019239824266 70-71 21.795533811152936 29.566968523783082 26.92000829062213 21.717489374441854 72-73 20.47640174373948 25.854637545664676 31.56994377724593 22.099016933349912 74-75 17.887561007188573 32.968113269053916 28.911714147833784 20.232611575923727 76-77 20.131583457874928 26.993948839115454 29.57839366672107 23.296074036288548 78-79 19.617065790920787 27.521081587084794 31.898276386832098 20.963576235162325 80-81 21.3998301686037 28.741713046325096 29.827610901947942 20.03084588312326 82-83 19.475816003016444 27.050130997396423 31.801645231116655 21.67240776847048 84-85 18.900051621313413 28.28730304985268 30.914413499510644 21.898231829323258 86-87 19.798412553860512 29.215035818326747 30.13083077439417 20.855720853418575 88-89 19.431294171102962 28.001434761340448 28.539163253735804 24.028107813820785 90-91 19.445767927538064 25.52457268839249 30.60497364662268 24.42468573744677 92-93 20.692873832851788 29.49411551581733 30.10815087287283 19.70485977845805 94-95 19.327705719644534 28.758351223481426 29.90913766500943 22.00480539186461 96-97 15.589751147998546 27.92636637798463 32.5856565814745 23.89822589254232 98-99 19.40556506218427 29.385290698150442 27.95892112955124 23.250223110114053 100-101 19.10672247796431 26.161008532865885 30.495031065116763 24.237237924053044 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8309.0 1 5383.0 2 5086.5 3 6439.5 4 3932.5 5 3201.0 6 5211.0 7 9181.5 8 12779.5 9 12282.0 10 8264.5 11 5305.0 12 4657.0 13 4520.0 14 4316.5 15 4129.0 16 3862.0 17 3485.5 18 3278.5 19 3148.0 20 3120.0 21 3313.0 22 3497.5 23 3705.5 24 4021.5 25 4456.0 26 5049.5 27 5501.0 28 5764.0 29 6239.0 30 6989.0 31 7862.5 32 9027.5 33 9832.0 34 10309.5 35 10824.0 36 11869.5 37 12987.0 38 13202.0 39 13303.5 40 13168.0 41 13098.0 42 12940.5 43 13294.0 44 15401.5 45 17591.5 46 25552.5 47 62850.0 48 163886.0 49 160746.0 50 64102.0 51 30404.5 52 16085.5 53 12284.5 54 11299.0 55 10436.5 56 9593.5 57 8822.5 58 8498.5 59 8378.5 60 7849.5 61 7318.0 62 6494.0 63 4558.5 64 2893.0 65 2284.5 66 1742.5 67 1343.0 68 1058.0 69 778.5 70 621.0 71 499.0 72 450.0 73 496.0 74 431.5 75 320.5 76 233.5 77 161.0 78 129.0 79 110.5 80 89.0 81 64.5 82 46.5 83 30.0 84 23.0 85 14.5 86 3.5 87 2.5 88 3.0 89 3.0 90 2.5 91 2.5 92 3.0 93 2.5 94 3.0 95 3.5 96 3.0 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33136557443658177 2 0.11313680184359015 3 0.10865613642404204 4 0.1149698013334053 5 0.13370712945151564 6 0.08503080966642464 7 0.09490864025042846 8 0.09979663888993551 9 0.08808580881611654 10-11 0.08635464263129114 12-13 0.09073347474584953 14-15 0.09159905783826224 16-17 0.0821794771267122 18-19 0.09694530635022307 20-21 0.10697588689171147 22-23 0.11537713455336421 24-25 0.12958288059943157 26-27 0.12810629767708048 28-29 0.12367654891002722 30-31 0.11328955180107475 32-33 0.10646672036676284 34-35 0.3263248258395901 36-37 0.48584669810600234 38-39 0.4617631214759311 40-41 0.4392579610732008 42-43 0.469757035917625 44-45 0.47734361713935985 46-47 0.4845228651411359 48-49 0.4962846118674497 50-51 0.4856430314960229 52-53 0.48717053107086883 54-55 0.4749505344721012 56-57 0.45229262411188625 58-59 0.43472637900115785 60-61 0.39480772344518367 62-63 0.463697954270736 64-65 0.49378969589520133 66-67 0.503362026564236 68-69 0.515785689772983 70-71 0.5080972752462584 72-73 0.49913594440716214 74-75 0.5051441094015562 76-77 0.48335178213375396 78-79 0.4627814545258285 80-81 0.4640543708382001 82-83 0.47734361713935985 84-85 0.47734361713935985 86-87 0.47902386667169045 88-89 0.49195669640538614 90-91 0.507893608636279 92-93 0.5026491934293078 94-95 0.5060606091464638 96-97 0.5061624424514535 98-99 0.4990850277546673 100-101 0.48630494797845614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 981997.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.306156600203668 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22476174524776 19.525682060832946 2 12.642559459213377 6.651542979229065 3 4.887052300332349 3.8567868937778504 4 2.455129485675024 2.583400828957787 5 1.4229743010216724 1.8716492400370734 6 0.8841313580149155 1.3954858775454662 7 0.639189960374881 1.1770241836429716 8 0.4260905813668601 0.8967044447446757 9 0.32102983235330457 0.7600554938900844 >10 1.828348416501831 8.913200436988854 >50 0.13572351523507756 2.490685957367323 >100 0.09035308281768556 4.828662336643849 >500 0.015123477381501015 2.8623098448059525 >1k 0.018613510623385867 12.360804323562027 >5k 0.004653377655846467 9.45381392306895 >10k+ 0.0042655961845259275 20.372191174905105 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 44046 4.485349751577653 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25115 2.557543454817072 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19633 1.9992932768633713 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17823 1.8149749948319596 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17403 1.772205006736273 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16089 1.6383960439797678 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15806 1.6095772186676742 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11879 1.209677829973004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11002 1.1203700214970107 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10889 1.1088628580331712 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10022 1.020573382607075 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9210 0.9378847389554144 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9204 0.9372737391254761 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8842 0.9004100827191935 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8818 0.8979660833994402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8622 0.878006755621453 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8244 0.839513766335335 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7515 0.7652772869978217 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7156 0.7287191305065087 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6622 0.6743401456419928 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6570 0.6690448137825269 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6092 0.6203684939974358 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5780 0.58859650284064 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4961 0.5051950260540511 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4950 0.504074859699164 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4779 0.48666136454592024 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4724 0.4810605327714851 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4698 0.47841286684175205 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4672 0.47576520091201907 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4381 0.4461317091600076 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3773 0.3842170597262517 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3744 0.3812638938815496 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3659 0.3726080629574225 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3588 0.36537789830315165 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3537 0.36018439974867544 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3362 0.34236357137547263 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3247 0.3306527413016537 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3184 0.3242372430873007 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3122 0.3179235781779374 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3047 0.31028608030370763 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3013 0.3068237479340568 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2881 0.2933817516754124 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2854 0.29063225244068974 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2649 0.26975642491779506 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2419 0.24633476477015714 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2399 0.24429809867036253 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2346 0.23890093350590683 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2340 0.23828993367596848 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2169 0.22087643852272462 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2069 0.2106931080237516 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1848 0.18818794762102126 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1736 0.17678261746217147 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1554 0.1582489559540406 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1543 0.15712878959915355 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1496 0.15234262426463624 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1493 0.15203712434966704 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1421 0.14470512639040647 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1410 0.14358496003551946 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1392 0.14175196054570433 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1355 0.1379841282610843 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1351 0.13757679504112538 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1332 0.1356419622463205 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1288 0.13116129682677238 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1277 0.13004113047188534 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1259 0.1282081309820702 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1212 0.12342196564755291 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1175 0.11965413336293287 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1148 0.11690463412821017 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1122 0.11425696819847718 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1121 0.11415513489348744 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1072 0.10916530294899068 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 995 0.10132413846478147 No Hit CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 987 0.10050947202486361 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0183330498973011E-4 0.0 12-13 0.0 0.0 0.0 2.0366660997946022E-4 0.0 14-15 0.0 0.0 0.0 2.5458326247432527E-4 0.0 16-17 0.0 0.0 0.0 5.091665249486505E-4 0.0 18-19 0.0 0.0 0.0 9.16499744907571E-4 0.0 20-21 0.0 0.0 0.0 0.0022403327097740623 0.0 22-23 0.0 0.0 0.0 0.004175165504578934 0.0 24-25 0.0 0.0 0.0 0.007077414696786243 0.0 26-27 0.0 0.0 0.0 0.010081497193983283 0.0 28-29 0.0 0.0 0.0 0.018380911550646285 0.0 30-31 0.0 0.0 0.0 0.037983822761169336 0.0 32-33 0.0 0.0 0.0 0.07708781187722569 0.0 34-35 0.0 0.0 0.0 0.140733627495807 0.0 36-37 0.0 0.0 0.0 0.2213346883951784 0.0 38-39 0.0 0.0 0.0 0.32199691037752665 0.0 40-41 0.0 0.0 0.0 0.43192596311394027 0.0 42-43 0.0 0.0 0.0 0.542211432417818 0.0 44-45 0.0 0.0 0.0 0.6733218125920954 0.0 46-47 0.0 0.0 0.0 0.837629850193025 0.0 48-49 0.0 0.0 0.0 1.0128340514278558 0.0 50-51 0.0 0.0 0.0 1.1890565857125837 0.0 52-53 0.0 0.0 0.0 1.3564196224632052 0.0 54-55 0.0 0.0 0.0 1.5420108208069883 0.0 56-57 0.0 0.0 0.0 1.8037733312830895 0.0 58-59 0.0 0.0 0.0 2.095220250163697 0.0 60-61 0.0 0.0 0.0 2.4676755631636347 0.0 62-63 0.0 0.0 0.0 2.7872284742214077 0.0 64-65 0.0 0.0 0.0 3.062636647566133 0.0 66-67 0.0 0.0 0.0 3.395122388357602 0.0 68-69 0.0 0.0 0.0 3.792628694385013 0.0 70-71 0.0 0.0 0.0 4.215389660049878 0.0 72-73 0.0 0.0 0.0 4.622875629966282 0.0 74-75 0.0 0.0 0.0 5.070331172091157 0.0 76-77 0.0 0.0 0.0 5.457806897577081 0.0 78-79 0.0 0.0 0.0 6.045079567452854 0.0 80-81 0.0 0.0 0.0 6.683472556433472 0.0 82-83 0.0 0.0 0.0 7.2391259851099345 0.0 84-85 0.0 0.0 0.0 7.93724420746703 0.0 86-87 0.0 0.0 0.0 8.652470425062399 0.0 88-89 0.0 0.0 0.0 9.297635328824832 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 50 4.3225737E-7 56.84366 2 TATCACG 85 1.8189894E-12 55.72908 2 ATCACGC 100 0.0 52.106686 3 TCACGCA 110 3.45608E-11 43.063377 4 CGTCACT 125 5.684342E-9 34.179394 1 GTAAGCG 265 0.0 34.10231 94-95 AGCACCG 70 2.0865045E-4 33.835514 5 TACCTGG 520 0.0 31.883461 2 TGTGCGA 40 4.5717577E-4 29.601543 18-19 TAGATAG 145 2.464185E-8 29.401896 5 CATTCCG 65 0.005934428 29.138708 9 GACATAT 115 3.881969E-6 28.895624 1 TAGTCAA 115 3.9598726E-6 28.821981 9 GAGTACG 220 1.8189894E-12 28.051271 1 GCTTATG 205 3.274181E-11 27.788128 1 TCGACCG 60 5.5069922E-6 27.628109 18-19 AGTACGC 225 3.6379788E-12 27.36917 2 AGTACTC 485 0.0 27.347464 5 TAACGCA 105 7.035124E-5 27.06841 4 ATAACGC 105 7.035124E-5 27.06841 3 >>END_MODULE