##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939205_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 981997 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.355627359350386 33.0 31.0 34.0 31.0 34.0 2 32.4977978547796 34.0 31.0 34.0 31.0 34.0 3 32.606495742858684 34.0 31.0 34.0 31.0 34.0 4 36.014839149203105 37.0 35.0 37.0 35.0 37.0 5 36.022614122039066 37.0 35.0 37.0 35.0 37.0 6 36.15923266568024 37.0 36.0 37.0 35.0 37.0 7 36.12817656265752 37.0 36.0 37.0 35.0 37.0 8 36.162066686558106 37.0 36.0 37.0 35.0 37.0 9 37.812236697260786 39.0 38.0 39.0 35.0 39.0 10-11 37.732493072789424 39.0 37.5 39.0 35.0 39.0 12-13 37.68975159801914 39.0 37.0 39.0 35.0 39.0 14-15 38.6797016691497 40.0 38.0 41.0 34.0 41.0 16-17 38.75205575984448 40.0 38.0 41.0 34.5 41.0 18-19 38.96453909736995 40.0 38.5 41.0 35.0 41.0 20-21 39.05120636824756 40.0 38.5 41.0 35.0 41.0 22-23 38.991215859111584 40.0 38.5 41.0 35.0 41.0 24-25 38.836825367083605 40.0 38.0 41.0 35.0 41.0 26-27 38.494686338145634 40.0 37.5 41.0 34.5 41.0 28-29 38.327359961384815 40.0 38.0 41.0 34.0 41.0 30-31 38.15897859158429 40.0 37.0 41.0 34.0 41.0 32-33 37.85750567466092 40.0 36.0 41.0 33.5 41.0 34-35 37.61736135650109 40.0 35.0 41.0 33.0 41.0 36-37 37.364798975964284 40.0 35.0 41.0 33.0 41.0 38-39 37.126301302346135 39.0 35.0 41.0 33.0 41.0 40-41 37.01497509666527 39.5 35.0 41.0 33.0 41.0 42-43 36.82078356654857 40.0 35.0 41.0 32.0 41.0 44-45 36.50214766440223 39.0 35.0 41.0 31.0 41.0 46-47 36.02001279026311 39.0 35.0 41.0 30.0 41.0 48-49 35.53539980264705 39.0 35.0 41.0 28.0 41.0 50-51 35.21316511150238 39.0 35.0 41.0 27.0 41.0 52-53 34.994449575711535 38.0 35.0 40.0 26.5 41.0 54-55 34.856550987426644 38.0 35.0 40.0 26.0 41.0 56-57 34.57531693070345 38.0 34.5 40.0 24.5 41.0 58-59 34.329909867341755 38.0 34.0 40.0 24.0 41.0 60-61 33.7018122254956 37.0 34.0 40.0 22.0 41.0 62-63 33.364836145120606 36.0 33.0 39.0 22.0 41.0 64-65 32.88528987359432 36.0 33.0 39.0 20.5 40.5 66-67 32.3961162814143 35.5 33.0 39.0 18.5 40.0 68-69 31.894907520084075 35.0 32.0 37.5 15.0 39.0 70-71 31.320575317439868 35.0 31.5 37.0 10.0 39.0 72-73 30.746999227085215 35.0 31.0 36.5 6.0 38.5 74-75 30.323833474033016 35.0 31.0 36.0 2.0 37.0 76-77 28.361741940148494 32.5 28.0 34.5 2.0 36.0 78-79 29.301517723577568 34.0 29.5 35.0 2.0 36.0 80-81 29.324222477258076 34.0 29.5 35.0 2.0 36.0 82-83 29.02221493548351 34.0 29.5 35.0 2.0 35.5 84-85 28.675803490234692 34.0 29.0 35.0 2.0 35.0 86-87 28.260523199154377 34.0 29.0 35.0 2.0 35.0 88-89 27.625344069279233 34.0 27.5 35.0 2.0 35.0 90-91 27.065181461857826 33.0 25.5 35.0 2.0 35.0 92-93 26.861151816146076 33.0 25.0 35.0 2.0 35.0 94-95 26.574504300929636 33.0 25.0 35.0 2.0 35.0 96-97 25.874606541567847 33.0 24.0 35.0 2.0 35.0 98-99 25.196383492006596 33.0 19.0 35.0 2.0 35.0 100-101 21.778246267554792 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13891894579269604 1101 2 0.16505252876660137 1101 3 0.1802996904150973 1101 4 0.1285207063991436 1101 5 0.10585837133204734 1101 6 0.1759684711150129 1101 7 0.10422886519418739 1101 8 0.20815835255409354 1101 9 0.286918414598091 1101 10-11 0.2614823670586759 1101 12-13 0.09664971820886592 1101 14-15 0.28696724691373277 1101 16-17 0.2881766950091489 1101 18-19 0.21660272786337487 1101 20-21 0.25514914599611416 1101 22-23 0.15308093621479202 1101 24-25 0.09271947430485028 1101 26-27 0.21192730848559194 1101 28-29 0.23506009953072038 1101 30-31 0.21359589113205857 1101 32-33 0.18292031534205933 1101 34-35 0.1949677360726909 1101 36-37 0.18609215841851068 1101 38-39 0.26255803687083556 1101 40-41 0.2644440544598794 1101 42-43 0.34801699267725184 1101 44-45 0.4097854311106488 1101 46-47 0.4334535614600057 1101 48-49 0.3903226477491799 1101 50-51 0.42022272205961286 1101 52-53 0.3316022053273784 1101 54-55 0.42845979434718373 1101 56-57 0.5656709765938928 1101 58-59 0.6088725661892482 1101 60-61 0.4886093790149246 1101 62-63 0.3271320797147155 1101 64-65 0.4312671308835121 1101 66-67 0.35784744658901246 1101 68-69 0.3684231451790936 1101 70-71 0.33070152508684103 1101 72-73 0.1854945351898003 1101 74-75 0.18838275590686493 1101 76-77 0.10467462711085673 1101 78-79 0.1883682149024004 1101 80-81 0.22003981250982818 1101 82-83 0.3425636091122257 1101 84-85 0.30189095554086265 1101 86-87 0.21234334925831533 1101 88-89 0.5595797215585989 1101 90-91 0.4971368677260273 1101 92-93 0.43641838207161143 1101 94-95 0.4458447487708419 1101 96-97 0.5840319688896329 1101 98-99 0.4719694991244765 1101 100-101 0.013110997347844489 1102 1 0.10219128053105209 1102 2 0.09659188656790718 1102 3 0.11937820040446923 1102 4 0.050610422310185754 1102 5 -0.023480924785289403 1102 6 0.06438045151322314 1102 7 0.060578887468956566 1102 8 0.01265442876807299 1102 9 0.15243878744343675 1102 10-11 0.13362091611457316 1102 12-13 0.14995066745666463 1102 14-15 0.1278140307799447 1102 16-17 0.20924262832191687 1102 18-19 0.4376712313416604 1102 20-21 0.26792286862384884 1102 22-23 0.2929664781770356 1102 24-25 0.2899711106435703 1102 26-27 0.33430573965654986 1102 28-29 0.20908857696466754 1102 30-31 0.15735638597459456 1102 32-33 0.18693405716036438 1102 34-35 0.3878500910296836 1102 36-37 0.3595140767900489 1102 38-39 0.30635794091799085 1102 40-41 0.5343885388846701 1102 42-43 0.3981395039003175 1102 44-45 0.42465383863609674 1102 46-47 0.4446209306385853 1102 48-49 0.5242411220989283 1102 50-51 0.2749655341633783 1102 52-53 0.40994513394532106 1102 54-55 0.3004141482075724 1102 56-57 0.3340819289258903 1102 58-59 0.28668946759769653 1102 60-61 0.3451598742001494 1102 62-63 0.3442022623678156 1102 64-65 0.3108891452079021 1102 66-67 0.28851294811396855 1102 68-69 0.3218474623717782 1102 70-71 0.33742679152733146 1102 72-73 0.08916473155044358 1102 74-75 0.1747051917384006 1102 76-77 0.4448874368158009 1102 78-79 0.5258566491575642 1102 80-81 0.5032849034936895 1102 82-83 0.3989530405916142 1102 84-85 0.31910678090667943 1102 86-87 0.3712181307942437 1102 88-89 0.4149779258696249 1102 90-91 0.5206924347045394 1102 92-93 0.26441433834452965 1102 94-95 0.4074636103588638 1102 96-97 0.5603093411524078 1102 98-99 0.7426507643312874 1102 100-101 0.548682088126089 1103 1 0.05875585228442759 1103 2 0.14634266129777984 1103 3 0.09242661897175708 1103 4 0.10011388468317506 1103 5 0.052102377121613586 1103 6 0.0963138056153241 1103 7 0.15444144191867082 1103 8 0.04835758835422865 1103 9 0.011701659983295087 1103 10-11 0.1397825463538993 1103 12-13 0.15519683206558454 1103 14-15 0.27939464367735667 1103 16-17 0.25713992674184993 1103 18-19 0.08961256502477966 1103 20-21 0.12550920933009024 1103 22-23 0.08912097598264523 1103 24-25 0.24965236464019114 1103 26-27 0.34835950345002686 1103 28-29 0.275524737583801 1103 30-31 0.2168596024015983 1103 32-33 0.27190088177901117 1103 34-35 0.20831361723932673 1103 36-37 0.14445275425648418 1103 38-39 0.021997236528676467 1103 40-41 0.12914059799915378 1103 42-43 0.13641676154787064 1103 44-45 0.0884121939679261 1103 46-47 -0.09939315386845493 1103 48-49 0.03060770733014806 1103 50-51 0.09051423966764816 1103 52-53 0.102272848779144 1103 54-55 -0.056038108210252346 1103 56-57 -0.20881924997522816 1103 58-59 -0.1332694594219248 1103 60-61 -0.14179815648297733 1103 62-63 -0.07777205540534027 1103 64-65 -0.2560163623426419 1103 66-67 -0.28990966361381965 1103 68-69 -0.3406087455782867 1103 70-71 -0.1353633837501782 1103 72-73 0.017170874734734554 1103 74-75 -0.05917919724977949 1103 76-77 -0.06446124354732419 1103 78-79 0.112349022099135 1103 80-81 -0.07206270017254823 1103 82-83 -0.027610219449812234 1103 84-85 -0.19769337977803048 1103 86-87 -0.33397529206342824 1103 88-89 -0.34670382548444323 1103 90-91 -2.218558505866497E-4 1103 92-93 -0.22133309151987035 1103 94-95 -0.2501167183342865 1103 96-97 -0.2382936856229101 1103 98-99 -0.2550209469609115 1103 100-101 -0.24380234895819086 1104 1 0.1911131911999604 1104 2 0.1927121863482384 1104 3 0.12428839552011794 1104 4 0.002030717783327418 1104 5 0.121918997685313 1104 6 0.1337800294087259 1104 7 0.15241353449484762 1104 8 0.2112750515875561 1104 9 0.20668782909395134 1104 10-11 0.27039300122461896 1104 12-13 0.1446474413723351 1104 14-15 0.3897987833319547 1104 16-17 0.34216785552612095 1104 18-19 0.36195893001074353 1104 20-21 0.22415375547399918 1104 22-23 0.22357497626033762 1104 24-25 0.2988708392906787 1104 26-27 0.40146557942913574 1104 28-29 0.3796936292970159 1104 30-31 0.5061875226416888 1104 32-33 0.3509625707495445 1104 34-35 0.3389581153222281 1104 36-37 0.35152693374831756 1104 38-39 0.5685930488130637 1104 40-41 0.5633597668641741 1104 42-43 0.5538739872530272 1104 44-45 0.3753203760871031 1104 46-47 0.4854692314526474 1104 48-49 0.2467801021566487 1104 50-51 0.1685806653172719 1104 52-53 0.3062737939789102 1104 54-55 0.3619557987222777 1104 56-57 0.20841969866357601 1104 58-59 0.35313862123511797 1104 60-61 0.47901653096028696 1104 62-63 0.4719023312828341 1104 64-65 0.5188779034454001 1104 66-67 0.6946727663506138 1104 68-69 0.7813006647665759 1104 70-71 0.6801471042292313 1104 72-73 0.4210201607617954 1104 74-75 0.44802861853773024 1104 76-77 0.6802887591227318 1104 78-79 0.8231926529478919 1104 80-81 0.6612187450752884 1104 82-83 0.7191763683256056 1104 84-85 0.7156292644316835 1104 86-87 0.7621547289766113 1104 88-89 0.8694024854595774 1104 90-91 0.7445935631879799 1104 92-93 0.7276121029135965 1104 94-95 0.42806812429261853 1104 96-97 0.8085891576661695 1104 98-99 0.7885223239643153 1104 100-101 0.6704247766732756 1105 1 -0.004183337465988757 1105 2 0.09116911944927608 1105 3 0.056349644878118 1105 4 -0.06466557321657973 1105 5 0.07682287385728159 1105 6 0.06193640560586999 1105 7 0.09179232985028563 1105 8 0.054127195113672144 1105 9 0.08756528002497532 1105 10-11 0.1936456271322271 1105 12-13 0.12962463147495384 1105 14-15 0.09343376455029784 1105 16-17 0.1754466398039085 1105 18-19 0.228958471798677 1105 20-21 0.2101109403399093 1105 22-23 0.23673409669457612 1105 24-25 0.09012880764886688 1105 26-27 0.11810043980033669 1105 28-29 0.07447215173933586 1105 30-31 0.23461567901787816 1105 32-33 0.19535551529077821 1105 34-35 0.3759184573196279 1105 36-37 0.22872798813960316 1105 38-39 0.36450677906211837 1105 40-41 0.36685350798945393 1105 42-43 0.37636275853974865 1105 44-45 0.4662779889532942 1105 46-47 0.2288176035409819 1105 48-49 0.39175953296661703 1105 50-51 0.6023646158102238 1105 52-53 0.4288977630017641 1105 54-55 0.30835568693867543 1105 56-57 0.4254611593026638 1105 58-59 0.4425717978028558 1105 60-61 0.6434945216751942 1105 62-63 0.6409592956027126 1105 64-65 0.6246984555154071 1105 66-67 0.6241666434289286 1105 68-69 0.4115537388156625 1105 70-71 0.6851641487110491 1105 72-73 0.5628638600941152 1105 74-75 0.4887251512319146 1105 76-77 0.3632972738106943 1105 78-79 0.5606723858757157 1105 80-81 0.41057800347668305 1105 82-83 0.20267695745540237 1105 84-85 0.35792614053315575 1105 86-87 0.5582327358044772 1105 88-89 0.6728118853528322 1105 90-91 0.8410692326307725 1105 92-93 0.6742990030837888 1105 94-95 0.7219016472888207 1105 96-97 0.6124498460920336 1105 98-99 0.682277548085402 1105 100-101 0.43877088287777255 1106 1 0.1881385507608755 1106 2 0.19158542860616024 1106 3 0.11490375548166298 1106 4 0.033845348607414394 1106 5 0.12534434122122917 1106 6 0.1131278119494894 1106 7 0.09710224888929986 1106 8 0.07469698981118711 1106 9 0.1163869597377527 1106 10-11 0.09513710202186587 1106 12-13 0.12833699707923074 1106 14-15 0.23703046475497302 1106 16-17 0.056201748399004714 1106 18-19 -0.002031394896114591 1106 20-21 0.14583407677523041 1106 22-23 0.24195114308056986 1106 24-25 0.1127154368628922 1106 26-27 0.14467999391573727 1106 28-29 0.21406274511769396 1106 30-31 0.1389921979351456 1106 32-33 0.29425410554382836 1106 34-35 0.09087610738985319 1106 36-37 0.25483359213539813 1106 38-39 0.22736323029618433 1106 40-41 0.30397512682571204 1106 42-43 0.47331581650666266 1106 44-45 0.39412971866151736 1106 46-47 0.1817203832494485 1106 48-49 0.23228999759352575 1106 50-51 0.26944551448196563 1106 52-53 0.5905297332441677 1106 54-55 0.3353768355397122 1106 56-57 0.2313003925460393 1106 58-59 0.08540095046872409 1106 60-61 0.18000006527380918 1106 62-63 0.1885628216477997 1106 64-65 0.0908802270702509 1106 66-67 0.2475177044540473 1106 68-69 -0.03267413592249113 1106 70-71 -0.02870119912400071 1106 72-73 -0.06530100024938079 1106 74-75 0.022174285778621794 1106 76-77 0.13414928153769878 1106 78-79 -0.026778301741426702 1106 80-81 -0.0926773328062076 1106 82-83 -0.02872035055585087 1106 84-85 0.06480147307717132 1106 86-87 0.19651232682926079 1106 88-89 0.1746136148213857 1106 90-91 0.35985587239033734 1106 92-93 0.2764307600186804 1106 94-95 -0.033371721867393234 1106 96-97 -0.11381729640217841 1106 98-99 0.12856779489897363 1106 100-101 -0.03602228073628311 1107 1 0.04629323828248744 1107 2 -0.018370857159503373 1107 3 -0.03892377618579701 1107 4 -0.01324083022438316 1107 5 0.13279835596787137 1107 6 0.08518620826421852 1107 7 0.022964429159792132 1107 8 0.04004262276839654 1107 9 0.11067482811548501 1107 10-11 0.08373704760963108 1107 12-13 0.13850297744471618 1107 14-15 0.13245061480362352 1107 16-17 0.029148954699756757 1107 18-19 0.14361066944865541 1107 20-21 0.15475848988396734 1107 22-23 0.13822033146217905 1107 24-25 0.09104046233980512 1107 26-27 0.20817169143905545 1107 28-29 0.14166104450486472 1107 30-31 0.21713312100639115 1107 32-33 0.23501336369297832 1107 34-35 0.17611771153625 1107 36-37 0.11866640363390957 1107 38-39 0.20675690279909276 1107 40-41 0.27910746844230516 1107 42-43 0.21736044375981578 1107 44-45 0.1672998767797793 1107 46-47 0.2279563709467496 1107 48-49 0.2929913389933958 1107 50-51 0.2728312925999532 1107 52-53 0.19541853400777853 1107 54-55 0.11776421835744344 1107 56-57 0.20209358917247755 1107 58-59 0.19123793049759286 1107 60-61 0.10565013006125668 1107 62-63 -0.07165445838613493 1107 64-65 -0.10596467039957957 1107 66-67 -0.10885878669473215 1107 68-69 -0.0781734834368244 1107 70-71 0.3257293630281417 1107 72-73 0.10069608085712645 1107 74-75 0.12980617780262094 1107 76-77 0.126151720865753 1107 78-79 0.3493157069184214 1107 80-81 0.10205678504699733 1107 82-83 0.04796573697406359 1107 84-85 0.3847309907911196 1107 86-87 0.18102213255914634 1107 88-89 0.38314215588186684 1107 90-91 0.24128216456557894 1107 92-93 0.3715316993213733 1107 94-95 0.23016043898992322 1107 96-97 0.2878700592094283 1107 98-99 0.5657764243101688 1107 100-101 0.5072440901983661 1108 1 -0.026415074055663013 1108 2 0.002997122965545884 1108 3 0.04943781118116419 1108 4 0.07278426759489065 1108 5 0.074876856758749 1108 6 0.11302362452083514 1108 7 0.08759854344074824 1108 8 0.08288702768105338 1108 9 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-0.7364320997073293 2316 100-101 -0.2146158545311927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 12.0 5 53.0 6 110.0 7 263.0 8 468.0 9 682.0 10 1227.0 11 1984.0 12 3127.0 13 4821.0 14 5112.0 15 7006.0 16 9376.0 17 13891.0 18 13259.0 19 7546.0 20 6507.0 21 6955.0 22 7523.0 23 7889.0 24 9029.0 25 9997.0 26 11396.0 27 13414.0 28 16159.0 29 19246.0 30 23328.0 31 27938.0 32 35101.0 33 44995.0 34 64466.0 35 110758.0 36 145221.0 37 211148.0 38 133780.0 39 8203.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.26157587151089 17.26020679226777 13.451612244063918 16.02660509215742 2 13.622037541866217 26.912505842685874 35.74359188470026 23.72186473074765 3 29.16200354990901 28.457113412770102 28.59316270823638 13.787720329084507 4 11.144331398161095 15.933755398438082 41.213466028918624 31.708447174482203 5 10.396365773011526 43.89738461522795 31.846431302743287 13.859818309017239 6 24.137751948325707 37.88168395626463 17.355653836009683 20.62491025939998 7 24.501296847139045 38.089322065138695 20.472058468610392 16.93732261911187 8 23.30719951282947 25.922278784965734 19.905457959647535 30.865063742557258 9 23.947731001214873 24.024716979787108 24.60435215178865 27.42319986720937 10-11 25.03688911473253 24.7446784460645 25.194170654289167 25.024261784913804 12-13 34.82968888906993 23.84095878093314 23.601854180817252 17.72749814917968 14-15 26.50212248551532 25.495284808176905 32.26417294349051 15.738419762817271 16-17 17.716905486143855 29.31288016187653 32.45677369160159 20.51344066037803 18-19 16.83122088520682 33.9701312581053 31.80062250731036 17.39802534937752 20-21 21.172060607109795 26.789134793690817 28.60853953728983 23.43026506190956 22-23 18.20137943394939 27.402273123033982 29.49184162477075 24.90450581824588 24-25 23.321880422651102 27.248185321265346 29.990111935738785 19.439822320344767 26-27 23.335507493214024 22.237429460731974 36.613768898515616 17.81329414753839 28-29 17.503770378117245 30.737059278185168 33.748982939866416 18.010187403831175 30-31 18.792236782611173 28.472785153019863 31.37788147019646 21.357096594172496 32-33 19.352554030205795 28.712613174989333 31.790117485083968 20.144715309720908 34-35 22.82331781752039 25.37194413085223 31.649966926417772 20.154771125209606 36-37 22.399570141104746 24.36780438364397 35.55712310313303 17.675502372118252 38-39 21.668638396324283 26.425183681513797 34.958333948703114 16.947843973458802 40-41 18.959504781187714 29.24056060431687 33.58258586430573 18.21734875018969 42-43 16.91552726145053 31.398444967122703 29.286130021279988 22.39989775014678 44-45 19.912797432498973 27.49869776616616 28.6565274884632 23.931977312871673 46-47 22.455332820766102 29.612621374053578 28.058982571016873 19.873063234163453 48-49 21.992648597566472 28.207175331304455 29.60128392411528 20.19889214701379 50-51 18.294158889568344 29.80336487961006 33.79121005013766 18.111266180683945 52-53 19.322491803679345 32.31695053592404 28.868564061647938 19.49199359874867 54-55 16.112982015220005 28.441736583716654 31.262722798542153 24.18255860252119 56-57 21.53148125707105 28.08913876518971 28.27753037942071 22.10184959831853 58-59 24.74128063286189 26.763477237176563 27.551550381281366 20.94369174868018 60-61 20.360868839035838 27.423190359992994 33.40847615051512 18.80746465045605 62-63 21.08928228636954 27.50839266628885 31.59334126645882 19.808983780882798 64-65 19.13416682026805 32.67244004199682 29.910517434690558 18.282875703044567 66-67 19.681565278405067 28.22606227649918 28.736928559680923 23.355443885414825 68-69 17.573439705406116 30.77257572085274 29.164468686160973 22.489515887580172 70-71 21.979100740175756 29.83162363613312 26.588434887497055 21.600840736194073 72-73 20.673128329312615 26.07782915833755 31.076367850410946 22.172674661938885 74-75 17.947814504524963 33.3732689611068 28.247947804321193 20.430968730047034 76-77 20.307547558743032 27.017100942296818 29.1638008624965 23.51155063646364 78-79 19.80647500183301 27.733372573299985 31.339410097027265 21.12074232783974 80-81 21.56986545916141 28.816220870403814 29.457788658405377 20.156125012029396 82-83 19.701657143497243 27.31562712896554 31.344075454866694 21.63864027267052 84-85 18.769100109267136 28.66475152164416 30.442353693544888 22.123794675543813 86-87 19.816517782963135 29.484681951584506 29.244918534466503 21.453881730985856 88-89 19.49848051506578 28.227403891619446 27.851686002080505 24.42242959123427 90-91 19.8676231194433 25.712905723638986 29.65681677046411 24.762654386453605 92-93 20.911469179641077 29.867708353487842 29.229315364507226 19.991507102363855 94-95 19.60056904450828 29.151769302757547 28.947186193033176 22.300475459700998 96-97 15.675251553721651 28.628295198457838 31.507020897212517 24.189432350607994 98-99 19.48889239652337 29.792257519208544 26.851988574163556 23.866861510104535 100-101 19.241060358649822 26.723530876379918 29.002834554579813 25.032574210390447 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7174.0 1 4715.0 2 4798.0 3 6252.5 4 4000.0 5 3259.5 6 5240.5 7 9279.5 8 12512.0 9 11948.0 10 8273.0 11 5302.0 12 4560.0 13 4381.0 14 4187.0 15 3818.0 16 3335.0 17 3052.0 18 3042.5 19 2998.5 20 2980.0 21 3114.0 22 3301.0 23 3508.5 24 3780.5 25 4226.5 26 4745.0 27 5208.5 28 5735.5 29 6345.0 30 7027.5 31 7816.5 32 8860.0 33 9726.0 34 10328.0 35 10899.0 36 12119.0 37 13169.0 38 13219.0 39 13413.0 40 13485.0 41 13389.5 42 13071.0 43 13458.5 44 15596.0 45 17708.5 46 24342.5 47 63630.0 48 164649.5 49 156702.0 50 61254.0 51 32816.5 52 20999.5 53 15548.5 54 12159.5 55 10464.5 56 9639.0 57 8986.5 58 8670.0 59 8464.0 60 7888.5 61 7333.0 62 6354.5 63 4409.0 64 2835.0 65 2181.0 66 1640.5 67 1324.5 68 1044.5 69 690.5 70 556.0 71 475.5 72 396.0 73 440.5 74 410.0 75 283.0 76 192.5 77 124.0 78 92.0 79 78.5 80 53.5 81 34.5 82 24.5 83 15.5 84 7.5 85 6.0 86 6.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3296344082517564 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011710830073818962 16-17 0.0014256662698562215 18-19 5.0916652494865056E-5 20-21 0.0 22-23 0.0 24-25 5.091665249486505E-4 26-27 8.65583092412706E-4 28-29 0.0 30-31 0.003360499064661094 32-33 0.0 34-35 0.009725080626519226 36-37 0.028462408744629568 38-39 0.02062124426042035 40-41 0.013034663038685454 42-43 0.009012247491591114 44-45 0.0031059158021867683 46-47 0.0012219996598767614 48-49 7.637497874229759E-4 50-51 5.0916652494865056E-5 52-53 1.5274995748459517E-4 54-55 0.0 56-57 0.0 58-59 0.004582498724537855 60-61 0.00906316414408598 62-63 0.0039205822421046095 64-65 0.0018329994898151419 66-67 0.0038187489371148795 68-69 0.0035641656746405537 70-71 5.0916652494865056E-5 72-73 0.0 74-75 0.0 76-77 0.20886010853393647 78-79 5.091665249486505E-4 80-81 0.0028004158872175777 82-83 6.109998299383807E-4 84-85 0.0 86-87 0.10488830413942202 88-89 0.39404397365776067 90-91 0.37978731095919843 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012219996598767614 100-101 8.65583092412706E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 981997.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.509840927165488 #Duplication Level Percentage of deduplicated Percentage of total 1 73.38328276943302 18.719958701614406 2 13.050166864552912 6.658153615754217 3 5.0680030965265646 3.8785185843232437 4 2.5517295927098704 2.603768639966783 5 1.4308768271402281 1.8250720123357242 6 0.9261166008284228 1.4175052300284172 7 0.6168398893368957 1.1014841218160203 8 0.4470990825132603 0.9124341178875928 9 0.3401960098144873 0.78105114849816 >10 1.8939641941615528 8.887357781085587 >50 0.15524083919764808 2.7525720425579294 >100 0.09178764260405506 4.778570123123687 >500 0.014366761451069488 2.52127373121032 >1k 0.020751988762655924 12.448674518538688 >5k 0.005187997190663981 10.368516796265393 >10k+ 0.004389843776715676 20.34508883499385 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 47681 4.855513815215321 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23778 2.4213923260458023 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19495 1.9852402807747884 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16297 1.6595773714176316 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 16225 1.6522453734583709 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 16105 1.6400253768596034 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15380 1.566196230742049 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12606 1.2837106427005378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11437 1.1646675091675434 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10537 1.0730175346767863 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10305 1.0493922079191687 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9854 1.0034653873688004 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9810 0.9989847219492525 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9651 0.9827932264558853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9057 0.9223042432919857 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8751 0.8911432519651282 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8305 0.8457255979397086 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7834 0.7977621112895458 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7347 0.7481692917595472 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6853 0.6978636390946205 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6786 0.6910408076603086 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6493 0.6612036492983175 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5900 0.6008164994394076 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5207 0.5302460190815247 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4955 0.5045840262241127 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4809 0.4897163636956121 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4623 0.47077536896752237 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4616 0.47006253583259416 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4463 0.45448204016916544 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4089 0.4163963841030064 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4038 0.4112028855485302 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3794 0.38635555913103603 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3705 0.37729239498695005 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3534 0.35987889983370625 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3491 0.3555000677191478 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3377 0.34389107095031857 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3353 0.3414470716305651 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3275 0.3335040738413661 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3072 0.3128319129284509 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3026 0.3081475808989233 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2821 0.2872717533760286 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2768 0.28187458821157296 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2766 0.28167092160159346 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2763 0.2813654216866243 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2557 0.2603877608587399 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2403 0.24470543189032148 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2362 0.24053026638574254 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2214 0.2254589372472625 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2185 0.22250577140256028 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1902 0.1936869460904667 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1849 0.18828978092601098 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1771 0.18034678313681202 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1734 0.17657895085219202 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1704 0.17352395170250012 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1593 0.16222045484864006 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1554 0.1582489559540406 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1542 0.15702695629416383 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1515 0.15427745705944113 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.1529536240945746 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1471 0.14979679163989298 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1420 0.14460329308541675 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1353 0.13778046165110483 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1305 0.1328924630115978 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1297 0.13207779657167995 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1285 0.13085579691180318 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1284 0.13075396360681346 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1243 0.12657879810223455 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1187 0.12087613302280964 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1170 0.11914496683798424 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1156 0.11771930056812802 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1126 0.1146643014184361 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1101 0.11211846879369285 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1092 0.11120196904878528 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1056 0.10753597006915501 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1006 0.1024443048196685 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1004 0.10224063820968904 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0183330498973011E-4 2 0.0 0.0 0.0 0.0 1.0183330498973011E-4 3 0.0 0.0 0.0 0.0 1.0183330498973011E-4 4 0.0 0.0 0.0 0.0 1.0183330498973011E-4 5 0.0 0.0 0.0 0.0 1.0183330498973011E-4 6 0.0 0.0 0.0 0.0 1.0183330498973011E-4 7 0.0 0.0 0.0 0.0 1.0183330498973011E-4 8 0.0 0.0 0.0 0.0 1.0183330498973011E-4 9 0.0 0.0 0.0 0.0 1.0183330498973011E-4 10-11 0.0 0.0 0.0 1.0183330498973011E-4 1.0183330498973011E-4 12-13 0.0 0.0 0.0 2.0366660997946022E-4 1.0183330498973011E-4 14-15 0.0 0.0 0.0 2.5458326247432527E-4 1.0183330498973011E-4 16-17 0.0 0.0 0.0 5.091665249486505E-4 1.0183330498973011E-4 18-19 0.0 0.0 0.0 9.16499744907571E-4 1.0183330498973011E-4 20-21 0.0 0.0 0.0 0.0022403327097740623 1.0183330498973011E-4 22-23 0.0 0.0 0.0 0.004226082157073799 1.0183330498973011E-4 24-25 0.0 0.0 0.0 0.007128331349281107 1.0183330498973011E-4 26-27 0.0 0.0 0.0 0.01018333049897301 1.0183330498973011E-4 28-29 0.0 0.0 0.0 0.01853366150813088 1.0183330498973011E-4 30-31 0.0 0.0 0.0 0.03844207263362312 1.0183330498973011E-4 32-33 0.0 0.0 0.0 0.07820797823211273 1.0183330498973011E-4 34-35 0.0 0.0 0.0 0.14256662698562214 1.0183330498973011E-4 36-37 0.0 0.0 0.0 0.22413510428239597 1.0183330498973011E-4 38-39 0.0 0.0 0.0 0.325611992704662 1.0183330498973011E-4 40-41 5.0916652494865056E-5 0.0 0.0 0.43543921213608594 1.0183330498973011E-4 42-43 1.0183330498973011E-4 0.0 0.0 0.5465393478798815 1.0183330498973011E-4 44-45 1.0183330498973011E-4 0.0 0.0 0.6798900607639331 1.0183330498973011E-4 46-47 1.0183330498973011E-4 0.0 0.0 0.8454200980247394 1.0183330498973011E-4 48-49 1.0183330498973011E-4 0.0 0.0 1.0222027154869109 1.0183330498973011E-4 50-51 1.0183330498973011E-4 0.0 0.0 1.2000036659989797 1.0183330498973011E-4 52-53 1.0183330498973011E-4 0.0 0.0 1.3686396190619727 1.0183330498973011E-4 54-55 1.0183330498973011E-4 0.0 0.0 1.555707400328107 1.0183330498973011E-4 56-57 1.0183330498973011E-4 0.0 0.0 1.8198120768189718 1.0183330498973011E-4 58-59 1.0183330498973011E-4 0.0 0.0 2.1133974951043637 1.0183330498973011E-4 60-61 1.0183330498973011E-4 0.0 0.0 2.488755057296509 1.0183330498973011E-4 62-63 1.0183330498973011E-4 0.0 0.0 2.8112611341989844 1.0183330498973011E-4 64-65 1.0183330498973011E-4 0.0 0.0 3.089469723430927 1.0183330498973011E-4 66-67 1.0183330498973011E-4 0.0 0.0 3.424552213499634 1.0183330498973011E-4 68-69 1.0183330498973011E-4 0.0 0.0 3.8247061854567783 1.0183330498973011E-4 70-71 1.0183330498973011E-4 0.0 0.0 4.24858731747653 1.0183330498973011E-4 72-73 1.0183330498973011E-4 0.0 0.0 4.656938870485348 1.0183330498973011E-4 74-75 1.0183330498973011E-4 0.0 0.0 5.106380162057521 1.0183330498973011E-4 76-77 1.0183330498973011E-4 0.0 0.0 5.494874220593342 1.0183330498973011E-4 78-79 1.0183330498973011E-4 0.0 0.0 6.0809758074617335 1.0183330498973011E-4 80-81 1.0183330498973011E-4 0.0 0.0 6.721303629237157 1.0183330498973011E-4 82-83 1.0183330498973011E-4 0.0 0.0 7.2777208077010425 1.0183330498973011E-4 84-85 1.0183330498973011E-4 0.0 0.0 7.978181196072901 1.0183330498973011E-4 86-87 1.0183330498973011E-4 0.0 0.0 8.6931019137533 1.0183330498973011E-4 88-89 1.0183330498973011E-4 0.0 0.0 9.33831773416823 1.0183330498973011E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATG 165 0.0 40.395123 1 GTAAGCG 420 0.0 34.45668 94-95 ACGGTCA 60 0.0039716894 31.632366 9 ACACCGT 75 3.0977544E-4 31.63236 6 GCACGGT 65 0.005874693 29.199104 7 TACCTGG 505 0.0 28.187256 2 GTGCTAG 85 6.3215574E-4 28.005026 1 CGCTTAC 70 0.00829807 27.204882 1 GCATAGA 70 0.00829807 27.204882 1 CGATCCG 35 0.008350866 27.17087 70-71 TTGCACG 70 0.008435463 27.113455 5 TTAGCGA 45 8.6939085E-4 26.520325 84-85 CATGGGG 2065 0.0 25.50503 4 ATGGGGA 800 0.0 25.503595 5 GTATAGT 95 0.0012102146 25.057127 1 CTTACAC 220 2.1955202E-9 23.724274 3 AGTACTC 550 0.0 23.292923 3 GTACTTG 145 2.2930677E-5 22.983435 1 GAGTACT 8840 0.0 22.973995 12-13 TAGATAG 145 2.3535296E-5 22.906197 5 >>END_MODULE