##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939204_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 965318 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29276880779184 33.0 31.0 34.0 31.0 34.0 2 32.28038635972809 34.0 31.0 34.0 31.0 34.0 3 32.363207771946655 34.0 31.0 34.0 30.0 34.0 4 35.75150882921483 37.0 35.0 37.0 35.0 37.0 5 35.83861794766077 37.0 35.0 37.0 35.0 37.0 6 36.01438282514156 37.0 37.0 37.0 35.0 37.0 7 35.987589581878716 37.0 37.0 37.0 35.0 37.0 8 36.01056646618006 37.0 37.0 37.0 35.0 37.0 9 37.636131305953064 39.0 38.0 39.0 35.0 39.0 10-11 37.54090517321753 39.0 37.5 39.0 35.0 39.0 12-13 37.48725963879261 39.0 37.5 39.0 35.0 39.0 14-15 38.54971729523328 40.0 38.0 41.0 34.0 41.0 16-17 38.584554519857704 40.0 38.0 41.0 34.5 41.0 18-19 38.65744759757924 40.0 38.0 41.0 35.0 41.0 20-21 38.65602008871688 40.0 38.0 41.0 35.0 41.0 22-23 38.66880913854295 40.0 38.0 41.0 35.0 41.0 24-25 38.54468682858912 40.0 38.0 41.0 34.5 41.0 26-27 38.30107591487986 40.0 38.0 41.0 34.5 41.0 28-29 38.16591579148012 40.0 38.0 41.0 34.0 41.0 30-31 37.96309817075824 40.0 37.0 41.0 34.0 41.0 32-33 37.63090919261839 40.0 35.5 41.0 33.5 41.0 34-35 37.163126037222966 40.0 35.0 41.0 33.0 41.0 36-37 36.790494427742985 39.0 35.0 41.0 32.0 41.0 38-39 36.52364195011385 39.0 35.0 41.0 31.0 41.0 40-41 36.42338327887805 39.0 35.0 41.0 31.0 41.0 42-43 36.21111540445739 39.0 35.0 41.0 31.0 41.0 44-45 35.83228013980885 39.0 35.0 41.0 30.0 41.0 46-47 35.392886592811905 39.0 35.0 41.0 27.5 41.0 48-49 35.08235731644909 39.0 35.0 41.0 24.5 41.0 50-51 33.8002021095639 38.0 34.0 40.0 23.0 40.5 52-53 34.00727687663547 37.5 34.0 39.5 23.0 40.5 54-55 34.59639362365563 38.0 35.0 40.0 23.0 41.0 56-57 34.417174444069204 38.0 35.0 40.5 22.5 41.0 58-59 34.24616758415361 38.0 35.0 40.0 22.0 41.0 60-61 33.74923237730986 37.5 34.0 40.0 20.5 41.0 62-63 33.37365614232823 37.0 34.0 40.0 19.0 41.0 64-65 32.93362394568422 36.0 33.0 39.5 14.0 41.0 66-67 32.36748874464166 36.0 33.0 39.0 8.0 40.0 68-69 31.857131535929092 35.0 32.5 38.0 4.5 39.5 70-71 31.234375615082286 35.0 31.5 37.0 2.0 39.0 72-73 30.702706776419788 35.0 31.0 37.0 2.0 39.0 74-75 30.316191659121657 35.0 31.0 36.0 2.0 37.5 76-77 29.863135256982673 35.0 31.0 35.5 2.0 37.0 78-79 29.409735444692835 34.0 30.0 35.0 2.0 36.5 80-81 28.84461234536184 34.0 29.0 35.0 2.0 36.0 82-83 28.5090498675048 34.0 29.0 35.0 2.0 36.0 84-85 28.145833290169666 34.0 29.0 35.0 2.0 35.0 86-87 27.842966255679475 34.0 28.0 35.0 2.0 35.0 88-89 27.297735046896463 34.0 26.5 35.0 2.0 35.0 90-91 26.722033050248726 33.0 25.0 35.0 2.0 35.0 92-93 26.29256214014449 33.0 24.5 35.0 2.0 35.0 94-95 25.79786453790357 33.0 23.0 35.0 2.0 35.0 96-97 25.10011623112798 33.0 18.0 35.0 2.0 35.0 98-99 24.428520964076085 32.5 2.0 35.0 2.0 35.0 100-101 21.60648770664175 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0965058211706733 1101 2 0.1974233294042378 1101 3 0.2575074476965753 1101 4 0.12776696880762017 1101 5 0.17908771494835918 1101 6 0.23370467620647872 1101 7 0.19197550867124136 1101 8 0.18679565684960409 1101 9 0.2523477594093677 1101 10-11 0.29960213779693134 1101 12-13 0.2793873340694475 1101 14-15 0.4576378470629052 1101 16-17 0.4266744149548245 1101 18-19 0.4686474239854306 1101 20-21 0.3766903670061623 1101 22-23 0.46124208206605033 1101 24-25 0.2615650644374554 1101 26-27 0.362894197883179 1101 28-29 0.28030894481994295 1101 30-31 0.3652542828344565 1101 32-33 0.5816365519130571 1101 34-35 0.5195719188232317 1101 36-37 0.4787983454837743 1101 38-39 0.42772179339419836 1101 40-41 0.28125398087835407 1101 42-43 0.31482813504944573 1101 44-45 0.1614874355416589 1101 46-47 0.5717344155939585 1101 48-49 0.4572065297884791 1101 50-51 0.3062179229920403 1101 52-53 0.2999163147828696 1101 54-55 0.391216163483314 1101 56-57 0.22530592442466002 1101 58-59 0.23743855062173225 1101 60-61 0.18648572619981252 1101 62-63 0.3229577235211636 1101 64-65 0.060060100930080296 1101 66-67 0.18593922859034961 1101 68-69 0.16233550311440226 1101 70-71 -0.29553551839740777 1101 72-73 -0.04777204051476147 1101 74-75 -0.17091645282435408 1101 76-77 -0.2569633301438863 1101 78-79 -0.17515547657794883 1101 80-81 -0.33238399822734266 1101 82-83 -0.20371953915092078 1101 84-85 -0.21872664862275215 1101 86-87 -0.3985225768824314 1101 88-89 -0.12946398048211094 1101 90-91 -0.09692621368149545 1101 92-93 -0.29625190802822843 1101 94-95 -0.8167207090764741 1101 96-97 -1.184942360311613 1101 98-99 -1.2989925931994826 1101 100-101 -1.782775239697525 1102 1 0.09423019038178637 1102 2 0.1986701662467354 1102 3 0.2905762632629916 1102 4 0.1775206724658247 1102 5 0.3176401558003832 1102 6 0.3035858888935081 1102 7 0.3226691921835183 1102 8 0.3224029830909103 1102 9 0.4604988784914994 1102 10-11 0.4045038035218198 1102 12-13 0.37395192390312815 1102 14-15 0.45501826128298006 1102 16-17 0.4110597796699551 1102 18-19 0.457690000699678 1102 20-21 0.42844330904974726 1102 22-23 0.4843377384058698 1102 24-25 0.49894775121729396 1102 26-27 0.358671536162916 1102 28-29 0.48331916519682494 1102 30-31 0.598894923573468 1102 32-33 0.6589496492277576 1102 34-35 0.550570862390046 1102 36-37 0.5194787007401445 1102 38-39 0.608410256161072 1102 40-41 0.6568528310859563 1102 42-43 0.5733210594039164 1102 44-45 0.6991520425730826 1102 46-47 0.9114452477193566 1102 48-49 1.0491853151359933 1102 50-51 0.8837644183363622 1102 52-53 1.1925762538537086 1102 54-55 1.0767568484451076 1102 56-57 1.0633108012150672 1102 58-59 1.109832784461993 1102 60-61 0.9873050761248905 1102 62-63 0.8712772577078383 1102 64-65 0.8997064414946507 1102 66-67 0.8707705063831455 1102 68-69 0.6355991446150249 1102 70-71 0.7416546150079917 1102 72-73 0.6250377032387711 1102 74-75 0.6689724988499712 1102 76-77 0.5936081879260335 1102 78-79 0.616314132541774 1102 80-81 0.5728349045562382 1102 82-83 0.32020990093948853 1102 84-85 0.23502522470353782 1102 86-87 0.3645814940705705 1102 88-89 0.28650658679794105 1102 90-91 0.20651118651013434 1102 92-93 0.029814352443974457 1102 94-95 -0.2374559742903415 1102 96-97 -0.20954589076391272 1102 98-99 -0.16203401520768423 1102 100-101 -0.4105176665456689 1103 1 0.06321945943856377 1103 2 0.15687150586121135 1103 3 0.2725627736023384 1103 4 0.1774958323194724 1103 5 0.05628090299198618 1103 6 0.15343597130434716 1103 7 0.15279035365031035 1103 8 0.06896773696889369 1103 9 0.06913068323041927 1103 10-11 0.2876082665655275 1103 12-13 0.3394438464720011 1103 14-15 0.3178612014211524 1103 16-17 0.3010654198443632 1103 18-19 0.2617421591622744 1103 20-21 0.41592972002850814 1103 22-23 0.43887661761847596 1103 24-25 0.47792495088531695 1103 26-27 0.5371041521517839 1103 28-29 0.4965560408324379 1103 30-31 0.5349987612299572 1103 32-33 0.4855493546528393 1103 34-35 0.580040043074348 1103 36-37 0.30710933068625224 1103 38-39 0.40874150681860755 1103 40-41 0.5556569972168575 1103 42-43 0.5635610736650918 1103 44-45 0.7218493610062282 1103 46-47 0.7319708067687642 1103 48-49 0.8111458456046279 1103 50-51 0.8038332109299091 1103 52-53 0.9799862448528032 1103 54-55 0.9818842930077452 1103 56-57 1.0080246719527892 1103 58-59 1.096751525037348 1103 60-61 1.2251772399646441 1103 62-63 0.9801180119131274 1103 64-65 1.0212785012980348 1103 66-67 1.1426042644342758 1103 68-69 1.181684922023937 1103 70-71 1.369183926380689 1103 72-73 1.3405639636570221 1103 74-75 1.2345387957394003 1103 76-77 1.005275896904724 1103 78-79 0.9321675522993331 1103 80-81 1.3987799990656882 1103 82-83 1.119618413219289 1103 84-85 0.9839841303343597 1103 86-87 0.8828302345290311 1103 88-89 0.6992095454054912 1103 90-91 0.7831057777874442 1103 92-93 0.9123803687858718 1103 94-95 0.719809476280691 1103 96-97 0.5546882436884921 1103 98-99 0.3421902708572837 1103 100-101 0.21111430184597424 1104 1 0.01808735701081332 1104 2 0.051685629857921356 1104 3 0.1767327060381234 1104 4 0.04699073771965345 1104 5 0.22630263069132894 1104 6 0.08456214078039892 1104 7 0.007829890126060945 1104 8 0.04778571043927826 1104 9 0.012866855385226472 1104 10-11 0.011507743711753449 1104 12-13 -0.05853517984926526 1104 14-15 0.0055676889748568215 1104 16-17 -0.11603199763502658 1104 18-19 -0.03408195560759708 1104 20-21 -0.03564925867041069 1104 22-23 0.09020657108746377 1104 24-25 0.07323414894470659 1104 26-27 0.1479352614550109 1104 28-29 0.03833473578148272 1104 30-31 0.1567334055008871 1104 32-33 0.2308937734120846 1104 34-35 0.19410550396945325 1104 36-37 0.30771949210619454 1104 38-39 0.020098446288614014 1104 40-41 0.09221707579374083 1104 42-43 0.09230810966956682 1104 44-45 0.19761652737690127 1104 46-47 0.28459846940406663 1104 48-49 0.2838026769321971 1104 50-51 0.382901201762607 1104 52-53 0.34929760576080326 1104 54-55 0.25241399249085106 1104 56-57 0.36360988124311433 1104 58-59 0.37438489243389483 1104 60-61 0.43659073098088896 1104 62-63 0.21349731404720274 1104 64-65 0.39692958817907 1104 66-67 0.39949214355286244 1104 68-69 0.25790549289691356 1104 70-71 0.20586901363872911 1104 72-73 0.20313874563592904 1104 74-75 0.25286348098540046 1104 76-77 0.20616286326151467 1104 78-79 0.2592388766968625 1104 80-81 0.20108273834321722 1104 82-83 0.16545492964403863 1104 84-85 0.1981706313810605 1104 86-87 0.23195666195950437 1104 88-89 0.48477005059930534 1104 90-91 0.37923844573065324 1104 92-93 0.36639007184064454 1104 94-95 -0.11369582666693745 1104 96-97 -0.2124037170627524 1104 98-99 -0.06376549988076263 1104 100-101 0.0651666665935231 1105 1 0.14550541284074114 1105 2 0.17180134012361492 1105 3 0.21569788934965572 1105 4 0.17072584741369923 1105 5 0.1864947627800646 1105 6 0.20622093087872173 1105 7 0.05551478686956557 1105 8 0.08607950897134486 1105 9 0.1140995233661144 1105 10-11 0.17210754174374188 1105 12-13 0.3143216729033398 1105 14-15 0.29863474318434413 1105 16-17 0.2538623352419691 1105 18-19 0.30162977442388694 1105 20-21 0.1145682506578325 1105 22-23 0.20155352644037805 1105 24-25 0.2645732852896927 1105 26-27 0.19031055449512735 1105 28-29 0.25961404151988887 1105 30-31 0.43127452229283136 1105 32-33 0.38459737222790835 1105 34-35 0.14527400359024512 1105 36-37 0.21429478044937866 1105 38-39 0.30215534269504474 1105 40-41 0.3528787627930541 1105 42-43 0.45885795170074317 1105 44-45 0.5428333252583073 1105 46-47 0.47802912129658637 1105 48-49 0.6272802290577673 1105 50-51 0.5366130892794061 1105 52-53 0.5122071884661494 1105 54-55 0.4175470192343411 1105 56-57 0.48842459423993745 1105 58-59 0.6001290451007151 1105 60-61 0.9021981477105214 1105 62-63 0.7378930332094384 1105 64-65 0.6048571780873004 1105 66-67 0.6014463429541408 1105 68-69 0.5567615140165252 1105 70-71 0.41569955114717416 1105 72-73 0.31454510334587127 1105 74-75 0.5705721524862213 1105 76-77 0.6705352635449913 1105 78-79 0.46176568719091193 1105 80-81 0.5311160174776575 1105 82-83 0.5719445680770896 1105 84-85 0.7244755146692405 1105 86-87 0.6852228936535667 1105 88-89 0.8067073410505827 1105 90-91 0.9633024510796311 1105 92-93 0.7972906253877277 1105 94-95 0.9743564253040482 1105 96-97 1.1945639031268662 1105 98-99 1.0242176795820441 1105 100-101 0.6366145783243873 1106 1 0.23471305921042784 1106 2 0.1774433821687751 1106 3 0.17261367693664198 1106 4 0.20456121512054182 1106 5 0.2964860087829706 1106 6 0.25126764916885946 1106 7 0.1434651826177742 1106 8 0.10872430709689951 1106 9 0.19190099725868492 1106 10-11 0.20752746085369012 1106 12-13 0.22783828601563272 1106 14-15 0.18149223486544486 1106 16-17 0.25549494271221107 1106 18-19 0.3544895000627051 1106 20-21 0.388152090311614 1106 22-23 0.42224841717126793 1106 24-25 0.3436287627721768 1106 26-27 0.39000884032222416 1106 28-29 0.2411332366716863 1106 30-31 0.16747268992797615 1106 32-33 0.04411883770416836 1106 34-35 0.14359658708441714 1106 36-37 0.17043387808632104 1106 38-39 0.016154204935183714 1106 40-41 0.24215109003148427 1106 42-43 0.2595135923141001 1106 44-45 0.24367716382109705 1106 46-47 0.24662616987478003 1106 48-49 0.239096306799496 1106 50-51 0.2800359021438865 1106 52-53 0.170987681339561 1106 54-55 0.0505558225863183 1106 56-57 0.05015650892352852 1106 58-59 0.05321918009930471 1106 60-61 0.2208891004415392 1106 62-63 0.24359806766693737 1106 64-65 0.2484006506446903 1106 66-67 0.04400570518519231 1106 68-69 0.16163673708758708 1106 70-71 0.2405650590998043 1106 72-73 0.2888091311716323 1106 74-75 0.2881252154760361 1106 76-77 0.21986676468664612 1106 78-79 0.35226863939200825 1106 80-81 0.34049967893434996 1106 82-83 0.46433859146091194 1106 84-85 0.8221994754243198 1106 86-87 0.6252060610419399 1106 88-89 0.5925097604787446 1106 90-91 0.4912191296520767 1106 92-93 0.6134112279328789 1106 94-95 0.7587152144989666 1106 96-97 0.6963374317669881 1106 98-99 0.5689546310610041 1106 100-101 0.5816319434491888 1107 1 0.055529360839372544 1107 2 0.1411454137714827 1107 3 0.11568731490869766 1107 4 -0.01497999651999038 1107 5 0.11103057592347199 1107 6 0.05003892608911542 1107 7 0.06789932330473647 1107 8 0.0013446475276239767 1107 9 0.09999446318510508 1107 10-11 0.11149671227214952 1107 12-13 0.15915730254909732 1107 14-15 0.07337924603010748 1107 16-17 0.025659057165270838 1107 18-19 0.17238895708180024 1107 20-21 0.12433841207356267 1107 22-23 0.14269805416718384 1107 24-25 0.09795430727830734 1107 26-27 0.20666859262532 1107 28-29 0.17049203113206346 1107 30-31 0.13958665490876143 1107 32-33 0.09666840759008011 1107 34-35 0.3326045792752481 1107 36-37 0.24690511230021883 1107 38-39 0.28694183229166015 1107 40-41 0.5085948701547238 1107 42-43 0.5746182229040571 1107 44-45 0.5328535411013107 1107 46-47 0.4849105942540106 1107 48-49 0.3552844588341486 1107 50-51 0.30256960966630686 1107 52-53 0.10318034804690512 1107 54-55 0.16857180696798935 1107 56-57 0.13197885045241975 1107 58-59 0.12200631938305406 1107 60-61 0.15047606889984166 1107 62-63 0.25891439980042463 1107 64-65 0.20177628361400224 1107 66-67 0.14020788931062356 1107 68-69 -0.12266684414601414 1107 70-71 0.018467566573420413 1107 72-73 0.015472544020145307 1107 74-75 0.1398956992781244 1107 76-77 0.149875916050199 1107 78-79 0.1455936348163256 1107 80-81 0.1698365101221988 1107 82-83 0.28485533845621447 1107 84-85 0.1108139589823729 1107 86-87 -0.044748182140292414 1107 88-89 0.03805060606473276 1107 90-91 -0.02632464437025206 1107 92-93 0.051544349768033015 1107 94-95 0.15488079317018943 1107 96-97 0.3027989045264228 1107 98-99 0.322002645215111 1107 100-101 -0.12720217283413504 1108 1 0.011198091668703114 1108 2 0.1112096536001772 1108 3 0.18797274809349318 1108 4 0.22612191910012314 1108 5 0.164980457282347 1108 6 0.14237413067272087 1108 7 0.09072471829287565 1108 8 0.14988565105792162 1108 9 0.1355497615241319 1108 10-11 0.22163097930149434 1108 12-13 0.2591775583509346 1108 14-15 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479.0 6 634.0 7 751.0 8 954.0 9 1281.0 10 1668.0 11 2493.0 12 4222.0 13 7307.0 14 6257.0 15 6696.0 16 9448.0 17 14122.0 18 11187.0 19 6899.0 20 6750.0 21 6996.0 22 7508.0 23 8278.0 24 8887.0 25 9908.0 26 11395.0 27 13222.0 28 15212.0 29 17813.0 30 21384.0 31 26347.0 32 33128.0 33 43320.0 34 62296.0 35 114461.0 36 145724.0 37 190246.0 38 129164.0 39 15129.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.77958643727244 16.852010354148057 13.058540040164443 16.309863168415063 2 13.38032332237753 26.100913006636752 36.3300514324652 24.18871223852052 3 28.958070367769768 28.646297358434207 28.347488961928637 14.048143311867392 4 11.052135316773319 15.532198052173696 41.50774493827596 31.907921692777023 5 10.454593548306788 43.39177574048833 32.163344142170445 13.990286569034446 6 24.265574120591292 37.11290829227128 17.96688150595534 20.65463608118209 7 24.392767644134416 38.21102976778617 20.657771547715445 16.73843104036396 8 23.023967337154918 25.622044517434045 20.350826990806837 31.003161154604204 9 24.145106191066162 23.817487182943076 24.97576552432001 27.06164110167075 10-11 24.83138466482988 24.477125284135827 25.368164864871872 25.32332518616242 12-13 34.59325095968668 23.728120310106455 23.707176978924476 17.97145175128239 14-15 26.82775365654404 25.09299461315485 32.16208847032674 15.917163259974373 16-17 17.886064230256043 29.1515000088123 32.4405092057423 20.521926555189353 18-19 16.892604022858304 33.79163649013156 31.87979638420739 17.435963102802752 20-21 21.182846481284965 26.86545112203123 28.411571642084297 23.540130754599506 22-23 18.084494161124347 27.387862687396698 29.438364658574574 25.08927849290438 24-25 23.284141776630822 27.057689505714816 29.74567337781158 19.912495339842774 26-27 23.360263658268853 22.23844045055448 36.31243463377022 18.088861257406442 28-29 17.67416996861036 30.390032053309497 33.80058299484306 18.135214983237084 30-31 18.601718882772857 28.687673390498087 31.315354381533496 21.395253345195563 32-33 19.23392377395349 28.729828570717558 31.64593056351946 20.39031709180949 34-35 22.76015188194536 25.42770068072465 31.28942699178744 20.522720445542546 36-37 22.269641145323206 24.38500763614759 35.397306181660646 17.94804503686856 38-39 21.88808356405005 26.258599165150827 34.605997628103744 17.247319642695384 40-41 18.956980656779496 29.30129674785111 33.6345632876969 18.10715930767249 42-43 16.919156291242473 31.439110164748634 29.086242486873992 22.555491057134898 44-45 19.617645894988513 27.65355914836189 28.65890739903823 24.069887557611363 46-47 22.295489440929757 29.495160805780895 28.060141481805058 20.14920827148429 48-49 21.936479208234232 28.04639044282733 29.57999925022181 20.43713109871663 50-51 18.305882181422845 29.64573408943107 33.895165115645774 18.153218613500314 52-53 19.27365066273871 32.17478369939253 29.06246046138923 19.489105176479534 54-55 16.031825615121853 28.351287566049553 31.589505377418952 24.02738144140964 56-57 21.37303103755726 27.9339576828247 28.353839915929864 22.339171363688177 58-59 24.41257720755848 26.787301633563093 27.756780837744948 21.04334032113348 60-61 20.552992275481806 27.121433587349475 33.52139197378345 18.804182163385263 62-63 21.052924733085895 27.407172194083383 31.806530871459582 19.73337220137114 64-65 19.112095565684747 32.43368209268105 30.33237838462668 18.12184395700752 66-67 19.466873024064558 28.24693939935208 28.98750346952262 23.298684107060748 68-69 17.34588383821286 30.584069303119897 29.625542114207647 22.44450474445959 70-71 21.774730305280777 29.582466640349057 26.93718250913738 21.705620545232783 72-73 20.537911570045665 25.970714281994805 31.39169647831951 22.099677669640023 74-75 17.912608492106187 32.958739280977305 28.913435966548995 20.215216260367516 76-77 20.14542319388527 26.983676595310452 29.536548172344084 23.334352038460196 78-79 19.623691733064618 27.56583755108669 31.83687235978356 20.973598356065132 80-81 21.40543348635434 28.806451764027347 29.76101550840748 20.027099241210834 82-83 19.45157526861848 27.130114996144925 31.841474099707057 21.576835635529534 84-85 18.85904726604878 28.31626544283159 30.90872461420251 21.91596267691712 86-87 19.780242090818415 29.224876257983105 30.0997640728563 20.89511757834218 88-89 19.46922512637063 27.988862620064463 28.52584343838491 24.01606881517999 90-91 19.453969345007955 25.572053811565787 30.506898284476453 24.467078558949805 92-93 20.711816196776393 29.508959933259423 30.084868229536355 19.694355640427837 94-95 19.34434868369323 28.76696707111134 29.851682790819257 22.037001454376174 96-97 15.617529050618245 27.90208060488816 32.587672591786415 23.89271775270718 98-99 19.378231866397964 29.44150224180219 27.958788333255225 23.221477558544628 100-101 19.155701999853186 26.16911470326548 30.40466141292832 24.270521883953016 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8229.0 1 5275.0 2 4864.5 3 6216.0 4 3893.0 5 3175.0 6 5128.5 7 8994.0 8 12444.0 9 11984.5 10 8126.5 11 5187.5 12 4562.5 13 4520.0 14 4353.0 15 4132.0 16 3901.5 17 3620.5 18 3294.0 19 3125.5 20 3060.0 21 3176.5 22 3401.5 23 3625.0 24 3943.5 25 4416.0 26 4975.0 27 5474.0 28 5829.0 29 6314.5 30 6971.5 31 7712.0 32 8769.5 33 9611.0 34 10348.0 35 10844.5 36 11656.5 37 12579.0 38 12765.0 39 12903.5 40 12966.5 41 13032.0 42 12670.0 43 13063.5 44 15246.0 45 17215.5 46 25142.0 47 61822.5 48 160784.0 49 157888.5 50 63075.0 51 29797.5 52 15780.5 53 12051.0 54 11063.5 55 10252.5 56 9396.5 57 8606.5 58 8234.0 59 8137.5 60 7714.0 61 7242.5 62 6440.0 63 4558.0 64 2871.5 65 2249.0 66 1782.0 67 1398.5 68 1057.5 69 784.5 70 664.0 71 520.5 72 449.5 73 475.5 74 410.5 75 297.0 76 211.0 77 147.0 78 132.5 79 112.5 80 81.5 81 63.5 82 45.0 83 29.0 84 25.0 85 16.0 86 6.5 87 5.5 88 3.5 89 2.5 90 1.5 91 1.0 92 0.5 93 0.5 94 1.0 95 1.0 96 1.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39064847024503846 2 0.1181993912886738 3 0.11964969056828942 4 0.13145927041658811 5 0.1338419049473852 6 0.07469041290020491 7 0.08484250785751431 8 0.08256346613240403 9 0.08111316685278841 10-11 0.07981825678170303 12-13 0.08396196900917625 14-15 0.07728023304237568 16-17 0.0784197539049308 18-19 0.0885200524593968 20-21 0.08696616037409434 22-23 0.09530538123188421 24-25 0.10633801503753167 26-27 0.10768472151146047 28-29 0.10224609921290187 30-31 0.09452843518923297 32-33 0.0929227467010871 34-35 0.33719458251063383 36-37 0.5265104349033168 38-39 0.5081227118939045 40-41 0.48124037881817183 42-43 0.5092104363536162 44-45 0.5183783996569006 46-47 0.5161511543346338 48-49 0.5214861838275056 50-51 0.5217451658417226 52-53 0.520294866562107 54-55 0.5137685198038366 56-57 0.486005647879766 58-59 0.47611253493667377 60-61 0.4240053536761979 62-63 0.49755624571384766 64-65 0.5238688183583027 66-67 0.5357301946094448 68-69 0.5494044449601064 70-71 0.5439658226615478 72-73 0.5307059435336335 74-75 0.5353158233866975 76-77 0.5126289989412816 78-79 0.4864718155053568 80-81 0.494603850751773 82-83 0.5070349874341927 84-85 0.5013891795242604 86-87 0.5026322931925024 88-89 0.5126289989412816 90-91 0.5299289974909822 92-93 0.5368697154719999 94-95 0.5296182190739218 96-97 0.532518817633153 98-99 0.5338137277042384 100-101 0.5099355859934239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 965318.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.65229905887443 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4819675787047 19.85115674401027 2 12.69523831563681 6.767145764240672 3 4.77617077529115 3.818877955779475 4 2.3995753054704014 2.558167946227484 5 1.3821281208096647 1.8418446006749654 6 0.8654667607282556 1.384000735946687 7 0.5850835717127643 1.0915675629406099 8 0.42878912355167315 0.9142572763273481 9 0.32366516959454067 0.7763778805477508 >10 1.793464497844971 8.808751524245068 >50 0.1388940996655941 2.577501761237046 >100 0.0879273570679504 4.819452335783617 >500 0.013617068572470194 2.588596929956156 >1k 0.019063896001458275 12.418815547035694 >5k 0.005057768326917501 10.487840862303937 >10k+ 0.00389059102070577 19.295644572743207 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 43226 4.477902618618942 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 24343 2.5217596688345187 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19515 2.0216136029785003 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17473 1.810077093765992 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 16967 1.757659134088456 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 15803 1.637077108268985 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15395 1.594811243548758 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11789 1.2212555862420467 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10802 1.1190094870291447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10771 1.1157981100528531 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9797 1.01489871731388 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8919 0.9239442339208428 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8899 0.9218723778071061 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8825 0.9142065101862806 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8802 0.9118238756554835 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8495 0.8800208843096265 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8108 0.839930468508823 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7398 0.7663795764711733 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6963 0.7213167059974019 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6752 0.6994586239974806 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6575 0.6811226973909116 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5990 0.6205209060641157 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5620 0.5821915679599883 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4883 0.5058436701687941 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4788 0.49600235362854517 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4697 0.4865754083110436 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4635 0.4801526543584601 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4603 0.4768376845764815 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4567 0.47310834357175563 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4244 0.4396478673349093 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3788 0.39240954794171456 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3621 0.3751095493920138 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3592 0.3721053580270957 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3545 0.3672364961598147 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3398 0.3520083537238506 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3254 0.337090989704947 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3190 0.3304610501409898 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3096 0.3207233264064277 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3046 0.31554368612208616 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3029 0.3137826084254101 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3027 0.3135754228140364 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2770 0.2869520717525209 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2740 0.28384428758191604 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2606 0.2699628516198807 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2447 0.25349159551567463 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2346 0.24302872214130472 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2290 0.23722752502284222 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2237 0.2317371063214402 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2148 0.22251734661531225 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2028 0.2100862099328926 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1884 0.19516884591398895 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1714 0.17755806894722775 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1518 0.15725387903260893 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.154767651696125 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1491 0.15445687327906452 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1489 0.15424968766769084 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1436 0.14875926896628883 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1434 0.14855208335491518 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1384 0.14337244307057365 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1360 0.1408862157340897 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1312 0.13591376106112182 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1312 0.13591376106112182 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1249 0.1293874143028515 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1226 0.1270047797720544 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1224 0.12679759416068073 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1144 0.11851016970573426 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1135 0.11757783445455279 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1133 0.11737064884317913 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1077 0.11156945172471663 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1073 0.1111550805019693 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1054 0.10918681719391952 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1007 0.10431795532663847 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 999 0.10348921288114382 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 968 0.10027783590485206 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 966 0.10007065029347842 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0359280568683067E-4 0.0 16-17 0.0 0.0 0.0 2.0718561137366133E-4 0.0 18-19 0.0 0.0 0.0 3.10778417060492E-4 0.0 20-21 0.0 0.0 0.0 6.733532369643993E-4 0.0 22-23 0.0 0.0 0.0 0.0025380237393273514 0.0 24-25 0.0 0.0 0.0 0.00466167625590738 0.0 26-27 0.0 0.0 0.0 0.007924849635042546 0.0 28-29 0.0 0.0 0.0 0.018335926606569027 0.0 30-31 0.0 0.0 0.0 0.04190328990032301 0.0 32-33 0.0 0.0 0.0 0.08069879563004109 0.0 34-35 0.0 0.0 0.0 0.14751615529804685 0.0 36-37 0.0 0.0 0.0 0.22635028042572497 0.0 38-39 0.0 0.0 0.0 0.32212182928319993 0.0 40-41 0.0 0.0 0.0 0.4344164306477244 0.0 42-43 0.0 0.0 0.0 0.5415831881307507 0.0 44-45 0.0 0.0 0.0 0.6641852736611148 0.0 46-47 0.0 0.0 0.0 0.8322128044851541 0.0 48-49 0.0 0.0 0.0 1.0022085986172433 0.0 50-51 0.0 0.0 0.0 1.1884684632421647 0.0 52-53 0.0 0.0 0.0 1.3629705444216311 0.0 54-55 0.0 0.0 0.0 1.5515612471745062 0.0 56-57 0.0 0.0 0.0 1.8211097275716397 0.0 58-59 0.0 0.0 0.0 2.120959103632171 0.0 60-61 0.0 0.0 0.0 2.480115360948413 0.0 62-63 0.0 0.0 0.0 2.7926030593027376 0.0 64-65 0.0 0.0 0.0 3.056816510206999 0.0 66-67 0.0 0.0 0.0 3.3756233697082205 0.0 68-69 0.0 0.0 0.0 3.761920942114412 0.0 70-71 0.0 0.0 0.0 4.193384977800061 0.0 72-73 0.0 0.0 0.0 4.593874764585349 0.0 74-75 0.0 0.0 0.0 5.045125026157184 0.0 76-77 0.0 0.0 0.0 5.433235472662894 0.0 78-79 0.0 0.0 0.0 6.00915967587883 0.0 80-81 0.0 0.0 0.0 6.642836868265173 0.0 82-83 0.0 0.0 0.0 7.187320654955155 0.0 84-85 0.0 0.0 0.0 7.866319699829486 0.0 86-87 0.0 0.0 0.0 8.57629299360418 0.0 88-89 0.0 0.0 0.0 9.221779765838821 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 350 0.0 39.411182 94-95 TATAACG 50 0.0016371091 37.898247 2 GCTTATG 215 0.0 37.554955 1 TACCTGG 520 0.0 37.351635 2 AGTACTC 435 0.0 34.84896 5 TCACGCA 55 0.0026135591 34.452953 4 TATCACG 60 0.004002833 31.581871 2 AAGTACT 475 0.0 29.919666 4 TCGCGAT 40 4.4642217E-4 29.72207 50-51 CCGGACA 80 4.5642155E-4 29.608006 4 GATTCGA 80 4.5822666E-4 29.588055 9 AAAGTAC 485 0.0 29.302769 3 GTATAGG 165 2.6484486E-9 28.785437 1 TCGACAG 35 0.008345437 27.174465 48-49 ACTCGTT 35 0.008347557 27.173052 82-83 ATCACGC 70 0.0085013695 27.070177 3 CCGTATC 75 1.0481199E-6 25.368118 94-95 CCTCTAG 175 1.486842E-7 24.426497 1 ATCGCGT 50 0.0016836182 23.677807 24-25 ACCTGGG 750 0.0 23.370584 3 >>END_MODULE