##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939203_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1023593 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29687971684058 33.0 31.0 34.0 31.0 34.0 2 32.36213123770874 34.0 31.0 34.0 31.0 34.0 3 32.42973232525037 34.0 31.0 34.0 31.0 34.0 4 35.84884128750392 37.0 35.0 37.0 35.0 37.0 5 35.894962157810774 37.0 35.0 37.0 35.0 37.0 6 36.011367799506246 37.0 37.0 37.0 35.0 37.0 7 35.95329589006568 37.0 37.0 37.0 35.0 37.0 8 35.948103396564846 37.0 37.0 37.0 35.0 37.0 9 37.65208144252647 39.0 38.0 39.0 35.0 39.0 10-11 37.571459066249965 39.0 38.0 39.0 35.0 39.0 12-13 37.51099216192373 39.0 38.0 39.0 35.0 39.0 14-15 38.82571539664691 40.5 38.0 41.0 35.5 41.0 16-17 38.80543927127286 40.0 38.0 41.0 35.0 41.0 18-19 38.769963745355824 40.0 38.5 41.0 35.0 41.0 20-21 38.678911930816255 40.0 38.5 41.0 35.0 41.0 22-23 38.596410878151765 40.0 38.0 41.0 34.5 41.0 24-25 38.60727994427472 40.0 38.0 41.0 34.5 41.0 26-27 38.50904802983217 40.0 38.0 41.0 34.5 41.0 28-29 38.405718874591756 40.0 38.0 41.0 34.0 41.0 30-31 38.25109296370725 40.0 38.0 41.0 34.0 41.0 32-33 38.01906128705453 40.0 38.0 41.0 34.0 41.0 34-35 37.664669942057046 40.0 37.0 41.0 33.0 41.0 36-37 37.40824526935999 40.0 37.0 41.0 33.0 41.0 38-39 37.172080602348785 40.0 36.5 41.0 32.5 41.0 40-41 37.05230154954167 40.0 36.0 41.0 31.5 41.0 42-43 36.825968915379455 40.0 36.0 41.0 31.0 41.0 44-45 36.521688796230535 40.0 35.5 41.0 30.5 41.0 46-47 36.10392460675288 39.0 35.0 41.0 29.5 41.0 48-49 36.01762077310025 39.0 35.0 41.0 29.0 41.0 50-51 34.95310001143032 38.0 34.0 40.0 27.0 40.5 52-53 35.11774259886498 38.5 35.0 39.5 27.5 40.5 54-55 35.60845081980826 39.0 35.0 41.0 28.0 41.0 56-57 35.42486369093966 39.0 35.0 41.0 28.0 41.0 58-59 35.22667065913893 39.0 35.0 41.0 27.5 41.0 60-61 34.87780836719282 38.0 35.0 40.5 26.5 41.0 62-63 34.53768978490474 37.5 35.0 40.0 26.0 41.0 64-65 34.153024200048264 37.0 34.0 40.0 26.0 41.0 66-67 33.700044842041706 36.5 34.0 39.0 24.5 41.0 68-69 33.255228884918125 36.0 34.0 39.0 23.0 41.0 70-71 32.785489935941335 35.0 33.0 38.5 22.0 40.0 72-73 32.34243297873276 35.0 33.0 37.0 20.5 39.5 74-75 31.92584210716564 35.0 33.0 37.0 20.0 39.0 76-77 31.517634938886843 35.0 32.0 36.0 18.0 39.0 78-79 31.102025414398106 35.0 32.0 36.0 14.0 37.5 80-81 30.66189979806427 35.0 31.0 35.5 7.5 37.0 82-83 30.272065655001548 35.0 31.0 35.0 4.0 37.0 84-85 29.926707197098846 34.0 31.0 35.0 2.0 36.0 86-87 29.62977423643968 34.0 31.0 35.0 2.0 36.0 88-89 29.247738603136206 34.0 30.0 35.0 2.0 35.5 90-91 28.892543716105912 34.0 30.0 35.0 2.0 35.0 92-93 28.509639085066034 34.0 29.0 35.0 2.0 35.0 94-95 28.075367846399885 34.0 29.0 35.0 2.0 35.0 96-97 27.55382998906792 34.0 28.0 35.0 2.0 35.0 98-99 26.948573798374937 34.0 26.5 35.0 2.0 35.0 100-101 24.54237231008809 31.5 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09137038392132979 1101 2 0.23420200527642976 1101 3 0.1281190458113386 1101 4 -8.341895817309819E-4 1101 5 0.033826556981502165 1101 6 0.10081814454689919 1101 7 0.10876100151490675 1101 8 0.17581850274360278 1101 9 0.1940427142254606 1101 10-11 0.12903046190897527 1101 12-13 2.1286583660184988E-4 1101 14-15 0.09664512937941794 1101 16-17 0.2378984414702714 1101 18-19 0.34184117726272234 1101 20-21 0.2383057115933127 1101 22-23 0.2973670697288924 1101 24-25 0.3097570716444338 1101 26-27 0.2841948382782107 1101 28-29 0.2542515581285869 1101 30-31 0.13435378464735237 1101 32-33 0.1321931811095567 1101 34-35 -0.056548360712859846 1101 36-37 -0.11889720415981486 1101 38-39 -0.04035935258956158 1101 40-41 -0.33083332315030844 1101 42-43 -0.05993276006068271 1101 44-45 -0.033548382428513435 1101 46-47 0.02632654679665336 1101 48-49 -0.030604564929994638 1101 50-51 0.0486824500788785 1101 52-53 -0.03410737188589508 1101 54-55 -0.0646327850800148 1101 56-57 -0.19671498743106497 1101 58-59 -0.1518356642206129 1101 60-61 -0.21673316197919945 1101 62-63 -0.1596127420830129 1101 64-65 -0.1525668123019983 1101 66-67 -0.14682899539074157 1101 68-69 -0.10186062037664101 1101 70-71 0.0011652866148565977 1101 72-73 -0.09788044772871274 1101 74-75 -0.3025497307918812 1101 76-77 -0.29832734871753175 1101 78-79 -0.3676420886723797 1101 80-81 -0.36394478883984505 1101 82-83 -0.2820817427270086 1101 84-85 -0.26204607332416074 1101 86-87 -0.6179016984624361 1101 88-89 -0.3931534041839235 1101 90-91 -0.36110377268425253 1101 92-93 -0.34624251151410235 1101 94-95 -0.4234259222385077 1101 96-97 -0.5697623335253006 1101 98-99 -0.6861374893363852 1101 100-101 -1.0091230146081003 1102 1 0.12689040498241866 1102 2 0.09449403593018957 1102 3 0.19750957682228432 1102 4 0.11789084665129224 1102 5 0.09020610633452009 1102 6 0.18388604325073743 1102 7 0.22247168850955035 1102 8 0.17354922519656668 1102 9 0.29531419523414115 1102 10-11 0.13504781811150934 1102 12-13 0.11076027591219884 1102 14-15 0.28535646767728196 1102 16-17 0.34233182823331987 1102 18-19 0.40086913119707646 1102 20-21 0.38094660219219634 1102 22-23 0.16012993913779638 1102 24-25 0.1361827058423799 1102 26-27 0.08164707718446351 1102 28-29 -0.02376716375396626 1102 30-31 0.2585717065924271 1102 32-33 0.1897951237756743 1102 34-35 0.27832707583169736 1102 36-37 0.1791549192943691 1102 38-39 0.15437345540487257 1102 40-41 0.2833648128884292 1102 42-43 0.20976984803166232 1102 44-45 0.13421635862017922 1102 46-47 0.3605198289124232 1102 48-49 0.24044795479078118 1102 50-51 0.3249323695229478 1102 52-53 0.473800820411725 1102 54-55 0.45001810953638 1102 56-57 0.21450278505706422 1102 58-59 0.16483070536037303 1102 60-61 0.16886782635630482 1102 62-63 0.055279614633704455 1102 64-65 -0.003299082799884445 1102 66-67 0.056890425922908605 1102 68-69 -0.039451374338966616 1102 70-71 -0.2150475016896607 1102 72-73 -0.3517406190968728 1102 74-75 -0.6091147301679989 1102 76-77 -0.5623478503718466 1102 78-79 -0.6207337222368459 1102 80-81 -0.6021963070619378 1102 82-83 -0.6430483864038123 1102 84-85 -0.5139372522104217 1102 86-87 -0.6557095077937625 1102 88-89 -0.5997111363872349 1102 90-91 -0.8652700565674429 1102 92-93 -0.9416508923468676 1102 94-95 -1.1016978982376422 1102 96-97 -0.9976836341572692 1102 98-99 -1.293548360204209 1102 100-101 -1.5117446814781559 1103 1 0.20884906661740388 1103 2 0.3249549655946069 1103 3 0.26180791863064456 1103 4 0.28648992382933613 1103 5 0.2784558016107468 1103 6 0.26445669948979855 1103 7 0.3314853981523527 1103 8 0.2803410855270556 1103 9 0.38919773546744096 1103 10-11 0.3210067191026198 1103 12-13 0.30790517352890845 1103 14-15 0.465909042121595 1103 16-17 0.4872431038584111 1103 18-19 0.3936806421456609 1103 20-21 0.38865673150954905 1103 22-23 0.28507384004435465 1103 24-25 0.3207848218026186 1103 26-27 0.34275339683677686 1103 28-29 0.1643121203630642 1103 30-31 0.10646803937029858 1103 32-33 0.1732158090887026 1103 34-35 0.18173842105218085 1103 36-37 0.03565701561178969 1103 38-39 0.04320749184684303 1103 40-41 -0.13296656006615137 1103 42-43 -0.06806798558721283 1103 44-45 -0.1620096195854046 1103 46-47 -0.014816603042149268 1103 48-49 0.24465229728338755 1103 50-51 0.31239934423054905 1103 52-53 0.2083223488046997 1103 54-55 0.23257412082254802 1103 56-57 0.0901722342387643 1103 58-59 0.08425159360577794 1103 60-61 0.07928359055929235 1103 62-63 -0.08761599617322702 1103 64-65 0.03015939216334118 1103 66-67 -0.07343118286249961 1103 68-69 -0.03807442615566714 1103 70-71 -0.012498879223223014 1103 72-73 -0.1418558612693417 1103 74-75 -0.2964935073443513 1103 76-77 -0.26510851115087064 1103 78-79 -0.2508413414368498 1103 80-81 -0.3270349693213319 1103 82-83 -0.498734850684464 1103 84-85 -0.36759309191466016 1103 86-87 -0.25529661846605123 1103 88-89 -0.23093098109193377 1103 90-91 -0.3578044867762671 1103 92-93 -0.405177700884078 1103 94-95 -0.6095719344714752 1103 96-97 -0.7575203256311163 1103 98-99 -0.8047460644666984 1103 100-101 -1.0519233400171224 1104 1 0.0922599613258015 1104 2 0.12664112606200462 1104 3 0.12545163540685422 1104 4 0.06635732334753897 1104 5 0.08333094472622093 1104 6 0.08266838623966066 1104 7 0.10934259744056618 1104 8 0.1409663668986596 1104 9 0.23566383355969123 1104 10-11 0.2004517633884788 1104 12-13 0.13614967808290146 1104 14-15 0.24392174036709235 1104 16-17 0.2765144282204588 1104 18-19 0.2729068592698667 1104 20-21 0.2889699866670341 1104 22-23 0.30237936916091 1104 24-25 0.25470967818553447 1104 26-27 0.20917226839086567 1104 28-29 0.16483316727233444 1104 30-31 0.09653617048601149 1104 32-33 0.09654729900697845 1104 34-35 0.1083473022998831 1104 36-37 -0.036901431304748655 1104 38-39 -0.0961020175414049 1104 40-41 -0.29296746296332543 1104 42-43 -0.3291237733461756 1104 44-45 -0.41085873919796256 1104 46-47 -0.2891330659926368 1104 48-49 -0.38304244321518155 1104 50-51 -0.23096605449260466 1104 52-53 -0.3401557051509343 1104 54-55 -0.44012565458178443 1104 56-57 -0.40936628498479166 1104 58-59 -0.4306963706746103 1104 60-61 -0.4457392013241659 1104 62-63 -0.4276696433134859 1104 64-65 -0.531634514033172 1104 66-67 -0.5569664767367186 1104 68-69 -0.5534130188650224 1104 70-71 -0.42500375424094017 1104 72-73 -0.2591497279641004 1104 74-75 -0.3330326272453341 1104 76-77 -0.346713486114286 1104 78-79 -0.40460911879243966 1104 80-81 -0.3084327795448836 1104 82-83 -0.5346576178636511 1104 84-85 -0.5241548344058806 1104 86-87 -0.4720916344736672 1104 88-89 -0.35242582768092845 1104 90-91 -0.46790527267301485 1104 92-93 -0.49061246468241393 1104 94-95 -0.6528232170243662 1104 96-97 -0.8029241401208616 1104 98-99 -0.6319459671544472 1104 100-101 -0.39727022182411886 1105 1 0.09134847986624806 1105 2 0.08955406786533615 1105 3 0.14137357608532142 1105 4 0.06451462605312486 1105 5 0.14481955799166712 1105 6 0.10164792169246084 1105 7 0.15526202198618222 1105 8 0.09840055275700621 1105 9 0.2412227604043622 1105 10-11 0.1036410851179923 1105 12-13 0.16068197338809398 1105 14-15 0.23402865134531936 1105 16-17 0.09854613149439473 1105 18-19 0.17925059755258133 1105 20-21 0.3468287082742023 1105 22-23 0.3778960397744484 1105 24-25 0.21053427905778932 1105 26-27 0.18147383924714688 1105 28-29 0.2571783264300649 1105 30-31 0.29567328492565537 1105 32-33 0.3602189042010622 1105 34-35 0.26166859766428274 1105 36-37 0.19959162219513615 1105 38-39 0.3940425120897615 1105 40-41 0.3691732480662111 1105 42-43 0.3677432712337705 1105 44-45 0.13937511314944118 1105 46-47 0.1983152546120195 1105 48-49 0.3503005106261128 1105 50-51 0.268440680849686 1105 52-53 0.26266100690479277 1105 54-55 0.2955471889443615 1105 56-57 0.13428491979882295 1105 58-59 0.23986166812316867 1105 60-61 0.3073097596609742 1105 62-63 0.20742448965673788 1105 64-65 0.22540634105731527 1105 66-67 0.10295905066445954 1105 68-69 0.12201879393469284 1105 70-71 0.24925447992715277 1105 72-73 0.2613943734311235 1105 74-75 0.369727595161077 1105 76-77 0.5740776649918296 1105 78-79 0.5120415136942889 1105 80-81 0.30590634089400837 1105 82-83 0.2525028275411998 1105 84-85 0.30116387575534986 1105 86-87 0.4210276055555404 1105 88-89 0.5031882320747592 1105 90-91 0.4204567495084248 1105 92-93 0.3064139349781563 1105 94-95 0.08269175149930419 1105 96-97 0.49850487533115384 1105 98-99 0.4807817182006744 1105 100-101 0.4711304797329241 1106 1 0.20518606295439668 1106 2 0.17158659483493466 1106 3 0.1449899539865953 1106 4 0.11751397224468718 1106 5 0.23991463263480028 1106 6 0.1851447071343273 1106 7 0.2044747276634169 1106 8 0.18812894983665984 1106 9 0.2210977195413335 1106 10-11 0.22142876082901353 1106 12-13 0.3853060335384697 1106 14-15 0.4070222767130858 1106 16-17 0.42103033112390165 1106 18-19 0.41585774040972723 1106 20-21 0.379777929152489 1106 22-23 0.43704867680180115 1106 24-25 0.2659990278863873 1106 26-27 0.3422357984930855 1106 28-29 0.3208258964420594 1106 30-31 0.40269355298711673 1106 32-33 0.24631662131994858 1106 34-35 0.2598161404342534 1106 36-37 0.29242560933690953 1106 38-39 0.5224239275850167 1106 40-41 0.05810685926813619 1106 42-43 0.1044117086316163 1106 44-45 0.05617798990655132 1106 46-47 0.22041193653422653 1106 48-49 0.26392288177137146 1106 50-51 0.3552405609848108 1106 52-53 0.31335499731560645 1106 54-55 0.24591223198674328 1106 56-57 0.3447908040747265 1106 58-59 0.2396505424829911 1106 60-61 0.2015562454406421 1106 62-63 0.1972223538825162 1106 64-65 0.005314671666440063 1106 66-67 -0.08410167179386008 1106 68-69 0.044144079975879436 1106 70-71 0.20055256208908645 1106 72-73 0.2811414312931717 1106 74-75 0.03811391421958987 1106 76-77 -0.012361258403618791 1106 78-79 0.16980685095047576 1106 80-81 0.26469938328258635 1106 82-83 0.209021161419372 1106 84-85 0.1642591138505871 1106 86-87 0.18115823103227413 1106 88-89 0.2815710096709232 1106 90-91 0.2529217435151807 1106 92-93 0.3632169479453573 1106 94-95 0.41049495516063317 1106 96-97 0.37144129246093627 1106 98-99 0.28308639293097926 1106 100-101 0.34881722376691826 1107 1 0.015619843759850482 1107 2 0.09141102881609697 1107 3 -0.006971075867141963 1107 4 0.16606386204632884 1107 5 0.13811681322653158 1107 6 0.11084429973434595 1107 7 0.19149928853966003 1107 8 0.10101798343046653 1107 9 0.15932193980447806 1107 10-11 0.1516479470308454 1107 12-13 0.17598863722510316 1107 14-15 0.23051811088125618 1107 16-17 0.1453946034808098 1107 18-19 0.23150194163860505 1107 20-21 0.11553980052094914 1107 22-23 0.24203723954807543 1107 24-25 0.22336499496045548 1107 26-27 0.2761700050649196 1107 28-29 0.3392736857962291 1107 30-31 0.3195219691361828 1107 32-33 0.1708265725655238 1107 34-35 0.15285068594950957 1107 36-37 0.18439991308365222 1107 38-39 0.39897672261607653 1107 40-41 0.17939183889813393 1107 42-43 0.22351046138657438 1107 44-45 0.4122157328438405 1107 46-47 0.5890640520087445 1107 48-49 0.3172908190584849 1107 50-51 0.3274478257280222 1107 52-53 0.40794609462409426 1107 54-55 0.5533729529511433 1107 56-57 0.59282163456961 1107 58-59 0.5402029952312688 1107 60-61 0.43857037086640815 1107 62-63 0.434716510030249 1107 64-65 0.2745273795406149 1107 66-67 0.2491460908455636 1107 68-69 0.2979791080263752 1107 70-71 0.32206503453949864 1107 72-73 0.4196428171209945 1107 74-75 0.38538796584585455 1107 76-77 0.3299580413359102 1107 78-79 0.3718851752675789 1107 80-81 0.43190556273894387 1107 82-83 0.43017861984453987 1107 84-85 0.27125796177685757 1107 86-87 0.18736001925107004 1107 88-89 0.2590951364324283 1107 90-91 0.394786475031907 1107 92-93 0.6134580068513849 1107 94-95 0.19773490373473024 1107 96-97 0.11106323696103715 1107 98-99 -0.04344720087119924 1107 100-101 0.0319061588500702 1108 1 0.0341373073840785 1108 2 0.07473981856903578 1108 3 0.11558556291414845 1108 4 -0.0891910938470275 1108 5 0.07661470554774041 1108 6 0.17456292868522638 1108 7 0.17956628668425623 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64-65 -0.8208981871551089 2316 66-67 -0.8709770564953274 2316 68-69 -0.68886610412126 2316 70-71 -0.7108075557927762 2316 72-73 -0.5835509007904705 2316 74-75 -0.611646871410759 2316 76-77 -0.6549013139871498 2316 78-79 -0.3854316173314736 2316 80-81 -0.47415826318375665 2316 82-83 -0.4903008313808783 2316 84-85 -0.7074697578782825 2316 86-87 -0.22870271687214938 2316 88-89 -0.3274137514877502 2316 90-91 -0.38336516451085956 2316 92-93 -0.493259093313295 2316 94-95 -0.3764713589795967 2316 96-97 -0.32970162063849884 2316 98-99 -0.3785908122244912 2316 100-101 -0.15498082894417564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3526.0 3 615.0 4 558.0 5 599.0 6 699.0 7 848.0 8 1048.0 9 1200.0 10 1598.0 11 2228.0 12 3336.0 13 5638.0 14 4920.0 15 5237.0 16 6787.0 17 9433.0 18 8845.0 19 5392.0 20 5519.0 21 5770.0 22 6093.0 23 6426.0 24 7201.0 25 8091.0 26 9404.0 27 11021.0 28 13151.0 29 15685.0 30 19025.0 31 23544.0 32 29556.0 33 39029.0 34 55126.0 35 88616.0 36 145408.0 37 229194.0 38 210764.0 39 32463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.51574320330245 18.28876051254251 14.261766842369946 21.933729441785097 2 15.691355126474557 21.95101433965217 37.37200931197058 24.985621221902694 3 21.67117890775075 24.17889601370887 29.941784265319775 24.208140813220606 4 13.274474782312698 16.337747398609853 37.09164679391747 33.29613102515997 5 13.640783917788813 37.727217584928994 33.26465443360453 15.367344063677669 6 31.178019020872895 35.25403210823475 16.59525201776533 16.972696853127022 7 27.583907122020673 33.54778899440537 20.70333242387704 18.164971459696908 8 25.828531527619674 31.331342197603597 20.0237254188622 22.816400855914534 9 25.574050062289395 18.356205373120318 21.86054607361687 34.209198490973414 10-11 25.924888330869184 25.210137444947705 26.26693615889481 22.598038065288307 12-13 28.05805414755951 23.48793284775601 26.517029839194272 21.936983165490208 14-15 23.71841478255895 25.23722375234755 27.291378291148 23.752983173945506 16-17 21.754674835600373 28.51839158962255 27.255065209856106 22.471868364920976 18-19 21.414167986050874 28.132416952139362 28.99763635456833 21.45577870724144 20-21 23.19898832920769 25.693298659027626 28.087654662693268 23.020058349071412 22-23 22.24517502161606 25.736902232811808 28.988358627057437 23.029564118514692 24-25 23.875518164810035 25.83949424668925 28.397412538671297 21.88757504982942 26-27 22.73116790120558 25.214332390309945 30.458139152687618 21.596360555796853 28-29 21.277714413157756 28.076830525617673 29.00664382931854 21.638811231906033 30-31 21.8530279252126 26.068438558027918 28.716788040589147 23.36174547617033 32-33 22.245580679344783 26.5521596955609 28.977461941444766 22.22479768364955 34-35 23.671564588909273 25.88427990937022 28.29230112493255 22.151854376787956 36-37 22.878551578798547 25.302797573201342 29.713262123807183 22.105388724192927 38-39 22.176974108460023 26.572126573009943 29.26232814795839 21.988571170571646 40-41 22.03746263647622 26.96679822813976 28.8113738197248 22.184365315659218 42-43 21.98331417509809 27.00987229915511 27.18313080575513 23.823682719991677 44-45 22.466280723834245 25.736012353835687 27.839669124540684 23.958037797789387 46-47 23.588422467427023 26.96255199747879 27.77526878658968 21.673756748504506 48-49 23.025426924104128 26.42888984022748 27.92469522253468 22.620988013133708 50-51 21.0778146223648 27.059646504686846 29.274723440602624 22.587815432345728 52-53 22.62814571617079 27.906743849948164 26.5854252411323 22.87968519274875 54-55 21.497899200911 26.332338562425146 27.723136277069877 24.446625959593977 56-57 22.827379389640008 26.134849623193073 27.413102781898544 23.624668205268378 58-59 24.28462928091517 25.633385569632427 27.098520538481996 22.983464610970408 60-61 22.669772802511066 26.28762000514965 29.082063291604847 21.96054390073444 62-63 21.531871412045955 26.730082790728265 28.705301031092777 23.032744766133 64-65 21.356331104675565 27.895860037870328 27.65736632839965 23.090442529054457 66-67 22.189332262350263 26.449975893061424 26.790271598294936 24.570420246293374 68-69 21.454396358484964 26.460055536050557 27.854889357554452 24.230658747910034 70-71 22.992475480869555 26.468042274786107 27.05601040912271 23.48347183522162 72-73 22.45149348991575 25.30709334066495 28.973802556901866 23.267610612517426 74-75 21.269407486765445 28.133355984198076 27.238041276291124 23.359195252745355 76-77 21.65057965757079 26.174053862477546 27.37504485430879 24.80032162564287 78-79 22.044750386367284 26.372456589200173 27.918269270881137 23.664523753551414 80-81 22.816157221956658 26.255829224697163 27.36415489370223 23.563858659643948 82-83 21.984275838913987 25.581581418453432 28.641347858163492 23.79279488446909 84-85 21.595912980009118 26.431284080383687 28.004951702736836 23.967851236870356 86-87 21.799290803814582 26.413336501023938 27.829127307783686 23.9582453873778 88-89 21.416326374317983 26.312363338952782 26.892586748775386 25.378723537953846 90-91 21.87754091985718 25.153486289343753 27.732704069006964 25.236268721792104 92-93 22.773195967395885 25.834226074456083 27.776490353977174 23.61608760417086 94-95 21.968442351097377 25.169525270324744 28.197142754737158 24.66488962384072 96-97 20.87889265201633 25.24841387288005 29.38722123097801 24.48547224412561 98-99 21.738451636279013 26.076970926142216 27.313342632019 24.87123480555977 100-101 21.30685458039718 24.428735877630263 28.513159606402223 25.751249935570332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2750.0 1 1690.0 2 810.0 3 954.0 4 770.5 5 778.5 6 1311.0 7 2330.0 8 3504.5 9 3722.0 10 2873.5 11 2267.0 12 2282.0 13 2362.5 14 2406.5 15 2466.0 16 2486.0 17 2472.0 18 2475.5 19 2499.0 20 2548.5 21 2699.5 22 2971.5 23 3310.5 24 3628.0 25 4031.0 26 4618.0 27 5249.5 28 5892.0 29 6631.0 30 7646.5 31 9185.0 32 11288.0 33 12717.5 34 13272.0 35 14003.5 36 15518.5 37 17127.5 38 18166.0 39 18776.5 40 19734.0 41 22578.5 42 25423.0 43 27535.5 44 29270.0 45 31281.5 46 35621.0 47 49103.0 48 75123.5 49 74690.0 50 49890.5 51 37874.0 52 34512.5 53 34199.5 54 33614.0 55 31245.0 56 28030.5 57 25024.5 58 23028.0 59 20697.0 60 17718.0 61 15246.5 62 13135.0 63 10727.0 64 8282.0 65 6428.0 66 4927.0 67 4119.5 68 3138.5 69 2235.5 70 1823.5 71 1512.5 72 1206.5 73 930.0 74 709.0 75 588.0 76 425.0 77 269.0 78 172.0 79 116.0 80 75.0 81 52.0 82 37.0 83 28.0 84 23.0 85 16.0 86 10.0 87 6.0 88 5.5 89 5.0 90 5.5 91 6.0 92 3.5 93 3.5 94 4.5 95 4.5 96 5.0 97 2.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34212817008322643 2 0.12241193521253077 3 0.11615945009393382 4 0.1226073253724869 5 0.1411693905683216 6 0.09056333913967758 7 0.09808586029798955 8 0.10365447985673994 9 0.09251724073923913 10-11 0.09075872929963374 12-13 0.09393381939892126 14-15 0.09598541607846087 16-17 0.08367583600122315 18-19 0.09964898157763878 20-21 0.11000466005531495 22-23 0.11772257137358305 24-25 0.13242568091028367 26-27 0.13179066289042618 28-29 0.1261243482516977 30-31 0.11738063859365978 32-33 0.10966272727539168 34-35 0.3320167293054955 36-37 0.493311306349301 38-39 0.46561475117551604 40-41 0.44461030898022946 42-43 0.47714277061292915 44-45 0.4847629868512192 46-47 0.49179703260964075 48-49 0.5026411864872073 50-51 0.493409001429279 52-53 0.4948255800889611 54-55 0.481929829531855 56-57 0.4594111135969081 58-59 0.440214030381216 60-61 0.401087150849996 62-63 0.47084143795434313 64-65 0.49995457178781016 66-67 0.5093821470056946 68-69 0.521252099223031 70-71 0.5134364928247848 72-73 0.5053766487265935 74-75 0.5115314387652123 76-77 0.4901850637900025 78-79 0.4697667920745843 80-81 0.469327164214683 82-83 0.4854956999510548 84-85 0.48393257867140554 86-87 0.48603302289093414 88-89 0.4988310783680623 90-91 0.5139738157646643 92-93 0.5074282454061332 94-95 0.5113848961452452 96-97 0.5111406584453001 98-99 0.5050347159466703 100-101 0.4924320506294983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1023593.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.70149374376001 #Duplication Level Percentage of deduplicated Percentage of total 1 66.18387743484277 24.290471636126615 2 14.706197679132153 10.794788442303332 3 6.63116803145156 7.3012131606042265 4 3.647939856468828 5.35539367279214 5 2.241528725319654 4.113372624438881 6 1.4849944536237976 3.2700908789515273 7 0.9956930863284961 2.5580396505013274 8 0.7189970356411527 2.1110612164292606 9 0.5349099751504339 1.7668795595812625 >10 2.5942784660001257 16.85139325722211 >50 0.16162933045338318 4.055755376131764 >100 0.08680380524565494 5.372226836375234 >500 0.003994040114002623 0.9996272400831014 >1k 0.006656733523337705 6.523034377584019 >5k 7.988080228005246E-4 1.9620213216490787 >10k+ 5.325386818670165E-4 2.674630749226161 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17111 1.6716605135048794 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10258 1.002156130415116 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7546 0.7372070735145707 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6712 0.6557293768128543 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5819 0.5684876703924314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4988 0.4873030589306492 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4655 0.45477059729794944 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4264 0.4165718210265213 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4180 0.4083654343083628 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4123 0.4027968147496124 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3929 0.38384396923386543 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3643 0.35590317636013535 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3615 0.35316771412074915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3553 0.3471106191621084 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2804 0.2739370042585285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2729 0.26660987326017277 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2503 0.2445307851851273 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2485 0.2427722737455219 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2360 0.23056038874826223 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2335 0.22811801174881033 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2181 0.21307296943218643 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1881 0.18376444543876325 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1628 0.15904759020430972 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1617 0.15797294432455086 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1412 0.13794545292904503 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 0.12592895809174154 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1282 0.125245092531895 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1202 0.11742948613364883 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1054 0.1029706142968934 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1037 0.10130979793726608 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.884753998903861E-5 0.0 12-13 0.0 0.0 0.0 9.769507997807723E-5 0.0 14-15 0.0 0.0 0.0 9.769507997807723E-5 9.769507997807723E-5 16-17 0.0 0.0 0.0 1.9539015995615445E-4 9.769507997807723E-5 18-19 0.0 0.0 0.0 2.9308523993423167E-4 9.769507997807723E-5 20-21 0.0 0.0 0.0 4.3962785990134755E-4 9.769507997807723E-5 22-23 0.0 0.0 0.0 0.0012211884997259653 9.769507997807723E-5 24-25 0.0 0.0 0.0 0.003712413039166935 9.769507997807723E-5 26-27 0.0 0.0 0.0 0.006887503138454444 9.769507997807723E-5 28-29 0.0 0.0 0.0 0.01958786353560448 1.9539015995615445E-4 30-31 0.0 0.0 0.0 0.0484567596691263 1.9539015995615445E-4 32-33 0.0 0.0 0.0 0.09940474387769357 1.9539015995615445E-4 34-35 0.0 0.0 0.0 0.1732133768011309 1.9539015995615445E-4 36-37 0.0 0.0 0.0 0.2742789370384518 1.9539015995615445E-4 38-39 0.0 0.0 0.0 0.4190630455659623 1.9539015995615445E-4 40-41 0.0 0.0 0.0 0.5672664818927053 1.9539015995615445E-4 42-43 0.0 0.0 0.0 0.7229924393777605 1.9539015995615445E-4 44-45 0.0 0.0 0.0 0.9199945681535532 1.9539015995615445E-4 46-47 0.0 0.0 0.0 1.1559770338405988 1.9539015995615445E-4 48-49 0.0 0.0 0.0 1.3771098473709764 1.9539015995615445E-4 50-51 0.0 0.0 0.0 1.6110895639184715 1.9539015995615445E-4 52-53 0.0 0.0 0.0 1.8376444543876325 1.9539015995615445E-4 54-55 0.0 0.0 0.0 2.1109952881662926 1.9539015995615445E-4 56-57 0.0 0.0 0.0 2.454393494289234 1.9539015995615445E-4 58-59 0.0 0.0 0.0 2.7867521563746527 1.9539015995615445E-4 60-61 0.0 0.0 0.0 3.1541833521722014 1.9539015995615445E-4 62-63 0.0 0.0 0.0 3.5147270448312953 1.9539015995615445E-4 64-65 0.0 0.0 0.0 3.893393174826323 1.9539015995615445E-4 66-67 0.0 0.0 0.0 4.342253219785598 1.9539015995615445E-4 68-69 0.0 0.0 0.0 4.8581320896098354 1.9539015995615445E-4 70-71 0.0 0.0 0.0 5.398727814668526 1.9539015995615445E-4 72-73 0.0 0.0 0.0 5.925939313770219 1.9539015995615445E-4 74-75 0.0 0.0 0.0 6.499897908641422 1.9539015995615445E-4 76-77 0.0 0.0 0.0 7.091588160528648 1.9539015995615445E-4 78-79 0.0 9.769507997807723E-5 0.0 7.845598787799448 1.9539015995615445E-4 80-81 0.0 9.769507997807723E-5 0.0 8.635854289742113 1.9539015995615445E-4 82-83 0.0 9.769507997807723E-5 0.0 9.375454892716148 1.9539015995615445E-4 84-85 0.0 9.769507997807723E-5 0.0 10.146220226203187 1.9539015995615445E-4 86-87 0.0 9.769507997807723E-5 0.0 10.941604719844705 1.9539015995615445E-4 88-89 0.0 9.769507997807723E-5 0.0 11.802786849851454 1.9539015995615445E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGC 65 0.005916322 29.157053 4 GTATTAC 70 0.008414358 27.127483 1 GTATAGG 200 7.221388E-10 26.1102 1 GTACATG 4950 0.0 25.223078 1 TACATGG 5130 0.0 23.828642 2 GAGTACT 4570 0.0 23.632486 12-13 CATGGGG 2140 0.0 23.468702 4 GTCCTAC 125 2.2643314E-4 22.787086 1 ACATGGG 5145 0.0 22.746183 3 GTACTTT 4905 0.0 21.922943 14-15 AGTACTT 4575 0.0 21.846514 12-13 GTAAGAC 160 5.0865572E-5 20.72884 3 CCTACAC 355 3.6379788E-12 20.019806 3 ACTTTTT 5575 0.0 19.672548 16-17 TATAAGC 370 7.2759576E-12 19.208193 3 GTGTAAG 250 2.1844244E-7 18.989239 1 CCTATAC 125 0.006119287 18.952084 3 CATGGGA 2765 0.0 18.849268 4 ATGGGAG 890 0.0 18.632666 5 TACTTTT 5400 0.0 18.59748 14-15 >>END_MODULE