##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939203_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1023593 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49779746442189 34.0 31.0 34.0 31.0 34.0 2 32.69212665580949 34.0 31.0 34.0 31.0 34.0 3 32.78399129341447 34.0 31.0 34.0 31.0 34.0 4 36.228509769019524 37.0 35.0 37.0 35.0 37.0 5 36.19290186626911 37.0 35.0 37.0 35.0 37.0 6 36.25328719520356 37.0 37.0 37.0 35.0 37.0 7 36.203534021823124 37.0 37.0 37.0 35.0 37.0 8 36.203759697457876 37.0 37.0 37.0 35.0 37.0 9 37.93330845365296 39.0 38.0 39.0 35.0 39.0 10-11 37.87160766046661 39.0 38.0 39.0 35.0 39.0 12-13 37.82374781773615 39.0 38.0 39.0 35.0 39.0 14-15 39.13481237171415 40.5 38.0 41.0 36.0 41.0 16-17 39.117006466437346 40.0 38.5 41.0 36.0 41.0 18-19 39.166155884223514 40.5 38.5 41.0 36.0 41.0 20-21 39.22064971135988 41.0 39.0 41.0 36.0 41.0 22-23 39.17639774793302 41.0 39.0 41.0 35.5 41.0 24-25 39.088410139576965 41.0 39.0 41.0 35.0 41.0 26-27 38.929601902318595 40.0 38.0 41.0 35.0 41.0 28-29 38.7909930997965 40.0 38.0 41.0 35.0 41.0 30-31 38.604773576997886 40.0 38.0 41.0 35.0 41.0 32-33 38.42128365473386 40.0 38.0 41.0 34.0 41.0 34-35 38.31122575085996 40.0 38.0 41.0 34.0 41.0 36-37 38.17900522961763 40.0 38.0 41.0 34.0 41.0 38-39 38.02509737757097 40.0 37.5 41.0 33.5 41.0 40-41 37.89581503585897 40.0 37.0 41.0 33.0 41.0 42-43 37.69398041995207 40.0 37.0 41.0 33.0 41.0 44-45 37.43244531762136 40.0 37.0 41.0 33.0 41.0 46-47 37.08819765277801 40.0 36.5 41.0 32.0 41.0 48-49 36.7214635113761 40.0 36.0 41.0 31.0 41.0 50-51 36.441216381901796 39.5 35.5 41.0 31.0 41.0 52-53 36.23807558277558 39.0 35.0 41.0 30.5 41.0 54-55 35.99907336216641 39.0 35.0 41.0 30.0 41.0 56-57 35.67659704589617 38.5 35.0 40.5 29.0 41.0 58-59 35.363518996319826 38.0 35.0 40.0 28.0 41.0 60-61 34.921978266752504 38.0 34.5 40.0 28.0 41.0 62-63 34.570946655555474 37.0 34.0 40.0 27.0 41.0 64-65 34.16195255340746 36.5 34.0 39.5 26.0 41.0 66-67 33.739483368878055 36.0 34.0 39.0 26.0 40.5 68-69 33.250220058167656 35.5 33.0 38.5 25.5 40.0 70-71 32.78769882169964 35.0 33.0 37.5 24.5 40.0 72-73 32.34968732689653 35.0 32.5 37.0 23.5 39.0 74-75 31.91367418495437 35.0 32.0 36.5 22.0 39.0 76-77 29.9812796687746 33.5 29.5 35.0 18.5 36.5 78-79 30.94459858557063 34.5 31.0 35.0 19.5 37.0 80-81 30.971867724769513 35.0 31.5 35.0 19.5 37.0 82-83 30.71733296339463 34.5 31.5 35.0 18.0 36.5 84-85 30.391426084390964 34.0 31.0 35.0 14.5 36.0 86-87 29.95605968387826 34.0 31.0 35.0 6.0 36.0 88-89 29.459878584554602 34.0 30.0 35.0 2.0 35.0 90-91 29.061743290546147 34.0 29.5 35.0 2.0 35.0 92-93 28.85215608156758 34.0 29.0 35.0 2.0 35.0 94-95 28.588810200929473 34.0 29.0 35.0 2.0 35.0 96-97 28.109229449595688 34.0 29.0 35.0 2.0 35.0 98-99 27.478051823332123 34.0 29.0 35.0 2.0 35.0 100-101 24.12866002405253 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16667673137882133 1101 2 0.14578349234849242 1101 3 0.08666567403066239 1101 4 0.09607822739282312 1101 5 0.093464755545547 1101 6 0.1034845250187999 1101 7 0.10940894282358471 1101 8 0.1518783307562117 1101 9 0.18005121977143546 1101 10-11 0.2691281734280082 1101 12-13 0.19627659764370975 1101 14-15 0.3060111475074905 1101 16-17 0.3064563545424761 1101 18-19 0.27968392899201433 1101 20-21 0.25826334189501665 1101 22-23 0.21793817163852935 1101 24-25 0.27829096182330204 1101 26-27 0.1923574797707417 1101 28-29 0.17860230418252598 1101 30-31 0.3029292906528056 1101 32-33 0.3277077274026965 1101 34-35 0.4170598224634432 1101 36-37 0.3271564536799474 1101 38-39 0.2508919089550403 1101 40-41 0.3049120070270064 1101 42-43 0.37273215837811335 1101 44-45 0.4626002551480539 1101 46-47 0.5014671511680504 1101 48-49 0.6755656538543207 1101 50-51 0.5459502638435794 1101 52-53 0.5167787038290541 1101 54-55 0.6088150108049675 1101 56-57 0.6886303964465768 1101 58-59 0.7191117374528346 1101 60-61 0.7887696720960946 1101 62-63 0.8218499765413938 1101 64-65 0.4981473849142617 1101 66-67 0.5075611506839337 1101 68-69 0.40194284488976706 1101 70-71 0.508279715744159 1101 72-73 0.4998069750484717 1101 74-75 0.6302106590013778 1101 76-77 0.4517522346410061 1101 78-79 0.7295211303291076 1101 80-81 0.528994469381356 1101 82-83 0.46639085309983486 1101 84-85 0.39788933925315106 1101 86-87 0.4591576898897536 1101 88-89 0.8455461514718188 1101 90-91 0.868627901873527 1101 92-93 0.7221894676335125 1101 94-95 0.6209505938954045 1101 96-97 0.6117266967020676 1101 98-99 0.45570947835719267 1101 100-101 -0.09465825259787763 1102 1 0.22367467600296465 1102 2 0.19217959165920462 1102 3 0.22154577253512997 1102 4 0.16250430036346586 1102 5 0.1543115594292317 1102 6 0.15686122372044764 1102 7 0.2307350784564548 1102 8 0.08033507119611016 1102 9 0.26183707949854096 1102 10-11 0.19324791998192126 1102 12-13 0.07514036831916115 1102 14-15 0.10780929287013663 1102 16-17 0.23614508644823928 1102 18-19 0.31537191772652307 1102 20-21 0.19339559758432756 1102 22-23 0.27328660515144065 1102 24-25 0.25037336333596727 1102 26-27 0.23440835820739636 1102 28-29 0.1458726032472626 1102 30-31 0.2134635375474616 1102 32-33 0.0625279060106152 1102 34-35 0.16485121895354382 1102 36-37 0.3559495408160984 1102 38-39 0.3804025265687443 1102 40-41 0.23012388624933777 1102 42-43 0.26795186695077433 1102 44-45 0.14201783923815725 1102 46-47 0.1731095459345866 1102 48-49 -0.06211203194877868 1102 50-51 -0.13225932416257535 1102 52-53 -0.06769806658450506 1102 54-55 -0.015473526121184022 1102 56-57 -0.1609137598552337 1102 58-59 -0.2078332939262637 1102 60-61 -0.11218904624885795 1102 62-63 -0.15699021010154013 1102 64-65 -0.4246937505746331 1102 66-67 -0.1860047961669835 1102 68-69 -0.21453965150750065 1102 70-71 -0.47020602482873386 1102 72-73 -0.4476301124880493 1102 74-75 -0.2078760452260653 1102 76-77 -0.10141810377615101 1102 78-79 -0.12342351438155319 1102 80-81 -0.1857127801034295 1102 82-83 -0.10370514213948212 1102 84-85 0.042946907602281925 1102 86-87 0.13654937816368928 1102 88-89 0.21028144127432924 1102 90-91 0.3585080209454681 1102 92-93 0.39500695053165913 1102 94-95 0.23850534107320343 1102 96-97 0.33539999203586035 1102 98-99 0.3699328438260565 1102 100-101 -0.041912289649491896 1103 1 0.1311830437112178 1103 2 0.11884685671620332 1103 3 0.1864879042876737 1103 4 0.17423665337733496 1103 5 0.22923263044386033 1103 6 0.17115754921356086 1103 7 0.2102857393525568 1103 8 0.18562445145885675 1103 9 0.2804157983968807 1103 10-11 0.2725931768930039 1103 12-13 0.28556823041360957 1103 14-15 0.3718763438074646 1103 16-17 0.41388652719003716 1103 18-19 0.46937027085226646 1103 20-21 0.22347772015287148 1103 22-23 0.20213474279162114 1103 24-25 0.19903277386946172 1103 26-27 0.35358559752059904 1103 28-29 0.2378237329691757 1103 30-31 0.29012384306474814 1103 32-33 0.32159124302534536 1103 34-35 0.3556388697381436 1103 36-37 0.35392737175521916 1103 38-39 0.29559045365358116 1103 40-41 0.24781477601673174 1103 42-43 0.04819692079939131 1103 44-45 0.27865376685373633 1103 46-47 0.42574929284149476 1103 48-49 0.3276039798056942 1103 50-51 0.29708768889404524 1103 52-53 0.3729207991015855 1103 54-55 0.3526457981140183 1103 56-57 0.37578577925413015 1103 58-59 0.5007863886927026 1103 60-61 0.5703388667087665 1103 62-63 0.5727162274076463 1103 64-65 0.4178346306884677 1103 66-67 0.36323622375031306 1103 68-69 0.31257588595759245 1103 70-71 0.45720857771650714 1103 72-73 0.4253244657833548 1103 74-75 0.4499702178913694 1103 76-77 0.27670929872850536 1103 78-79 0.42316962832543226 1103 80-81 0.42323653753646795 1103 82-83 0.5014476490261934 1103 84-85 0.48455962157560606 1103 86-87 0.6321668194206218 1103 88-89 0.605782976926033 1103 90-91 0.6609084767627955 1103 92-93 0.7585720040160489 1103 94-95 0.5630247751000219 1103 96-97 0.592895156131398 1103 98-99 0.7309814058030355 1103 100-101 0.3048867773819346 1104 1 0.12246883198434233 1104 2 0.12421157074233236 1104 3 0.14600299834573605 1104 4 0.13080479139018308 1104 5 0.13199812923811294 1104 6 0.21564529928159715 1104 7 0.17598317098631355 1104 8 0.17589824248581465 1104 9 0.13982593065878035 1104 10-11 0.0823797044131922 1104 12-13 0.17154423090518378 1104 14-15 0.12928793826117158 1104 16-17 0.1940160176926895 1104 18-19 0.21391996969844485 1104 20-21 0.3020423142943258 1104 22-23 0.24014984876290413 1104 24-25 0.22839135321092385 1104 26-27 0.2788645864764092 1104 28-29 0.2243699381049069 1104 30-31 0.1151636896725492 1104 32-33 0.25387082011834394 1104 34-35 0.3069313294181413 1104 36-37 0.3113476189734783 1104 38-39 0.4981065271042624 1104 40-41 0.5219067686982513 1104 42-43 0.45254562470304904 1104 44-45 0.42340135066329054 1104 46-47 0.5383449591858778 1104 48-49 0.16765198383863122 1104 50-51 0.18944980583678728 1104 52-53 0.1326260031751545 1104 54-55 0.17694765168666748 1104 56-57 0.2930070379680316 1104 58-59 0.35786466360733726 1104 60-61 0.3430213365839734 1104 62-63 0.2446265947990085 1104 64-65 0.19082747146631363 1104 66-67 0.20841067727806006 1104 68-69 0.2170217767095508 1104 70-71 0.28830097043162795 1104 72-73 0.3546558328072891 1104 74-75 0.23123585400123403 1104 76-77 0.25554952731197034 1104 78-79 0.1385958773018352 1104 80-81 0.18569557798250003 1104 82-83 0.09298511988581382 1104 84-85 0.1095400274529652 1104 86-87 0.22198815864284782 1104 88-89 0.1606885301198453 1104 90-91 0.02206546424638489 1104 92-93 0.032458336786326925 1104 94-95 -0.07265872432877174 1104 96-97 -0.22345430520265808 1104 98-99 -0.15853909309781145 1104 100-101 -0.38267380764527203 1105 1 0.045821751685167555 1105 2 0.11699125617639083 1105 3 0.08339939287536424 1105 4 0.05323389564689052 1105 5 0.0464779804409261 1105 6 0.11671070886855262 1105 7 0.14386917536734245 1105 8 0.05592310779870502 1105 9 0.07342099114463707 1105 10-11 0.08550390270418973 1105 12-13 0.07216500034847684 1105 14-15 0.13159624347301246 1105 16-17 0.11179952666196158 1105 18-19 0.05146042516782501 1105 20-21 0.21064070495602039 1105 22-23 0.15478942506586435 1105 24-25 0.06539836384774844 1105 26-27 0.1289974234901976 1105 28-29 0.19504773989283564 1105 30-31 0.18980773087792358 1105 32-33 0.1628127122502434 1105 34-35 0.27648797200886577 1105 36-37 0.28077783822239155 1105 38-39 0.22520231776201172 1105 40-41 0.22464910682703731 1105 42-43 0.3358901819623341 1105 44-45 0.25936798970182906 1105 46-47 0.260595344006191 1105 48-49 0.272773917610067 1105 50-51 0.1432158646846915 1105 52-53 0.059232628004942 1105 54-55 0.13313302160752727 1105 56-57 0.10637165028622775 1105 58-59 0.04250256248840145 1105 60-61 0.013016945685471626 1105 62-63 -0.07797247176502964 1105 64-65 -0.10983252004790955 1105 66-67 -0.4010731701357457 1105 68-69 -0.12325003964235748 1105 70-71 -0.044863387313128555 1105 72-73 -0.11800527369913283 1105 74-75 -0.08363412113344992 1105 76-77 -0.02487162892507655 1105 78-79 0.20487069327878515 1105 80-81 0.1834625100765095 1105 82-83 0.14678041027714883 1105 84-85 0.1525813166997061 1105 86-87 -0.10909792270224372 1105 88-89 0.058131448439265654 1105 90-91 0.01582508475434352 1105 92-93 -0.053691461923044415 1105 94-95 0.006533473685951918 1105 96-97 -0.0395034092620179 1105 98-99 -0.013706662612150922 1105 100-101 0.31806117449511717 1106 1 0.11947735809248883 1106 2 0.17331412857478057 1106 3 0.1708803234627041 1106 4 0.11085076390448734 1106 5 0.16743935125927578 1106 6 0.12497185085395301 1106 7 0.14482945650497214 1106 8 0.07597323311198778 1106 9 0.21894104126995018 1106 10-11 0.27406634140965025 1106 12-13 0.1988506002177175 1106 14-15 0.18769096396991358 1106 16-17 0.1560031062631424 1106 18-19 0.17887815427319964 1106 20-21 0.16845303469340678 1106 22-23 0.2146367335544781 1106 24-25 0.33109998202361623 1106 26-27 0.2058709932842575 1106 28-29 0.4126127121059824 1106 30-31 0.34514852852421285 1106 32-33 0.37458535036728335 1106 34-35 0.1650034182331268 1106 36-37 0.2653384244706203 1106 38-39 0.4925963463116503 1106 40-41 0.40528411826867483 1106 42-43 0.40299067776706465 1106 44-45 0.5904470460383209 1106 46-47 0.8691718123509702 1106 48-49 0.8830666330944297 1106 50-51 0.8328816051227435 1106 52-53 0.9289408659912155 1106 54-55 0.9525422784452715 1106 56-57 0.8195268208212596 1106 58-59 0.8432374159263389 1106 60-61 0.9325780767740639 1106 62-63 0.9124996324084478 1106 64-65 1.0407443296851184 1106 66-67 1.0105128601573767 1106 68-69 0.90048797386968 1106 70-71 0.9461399362130791 1106 72-73 0.8020564294718397 1106 74-75 0.7149009393438277 1106 76-77 0.8619139491505479 1106 78-79 0.9311326797667441 1106 80-81 0.8166112787372946 1106 82-83 0.794408311552079 1106 84-85 0.8270902793236523 1106 86-87 1.049191823402147 1106 88-89 1.264884744723453 1106 90-91 1.521515261282623 1106 92-93 1.5015247034904888 1106 94-95 1.2149996118574684 1106 96-97 1.2067269764849584 1106 98-99 1.2743402209009815 1106 100-101 0.9397410535405584 1107 1 0.008824015408990249 1107 2 -0.014605376534419179 1107 3 0.001905335150411247 1107 4 0.04495715540920031 1107 5 -0.059333423067847946 1107 6 0.061125677742353446 1107 7 0.12195703614833064 1107 8 -0.05613122157311068 1107 9 0.15346485079376038 1107 10-11 0.006839499761348122 1107 12-13 0.004368240303200821 1107 14-15 0.09261774884936358 1107 16-17 0.07834770153463211 1107 18-19 0.11535756721649193 1107 20-21 0.20014445424860128 1107 22-23 0.09262544657124039 1107 24-25 -0.018076452224690343 1107 26-27 0.03498214335437666 1107 28-29 0.034287977583844054 1107 30-31 -0.03255007896927253 1107 32-33 -0.010335632401705652 1107 34-35 -0.0749816799435905 1107 36-37 -0.06611131005151805 1107 38-39 -0.0658630472634556 1107 40-41 0.11746708928317418 1107 42-43 0.21676171563522928 1107 44-45 -0.07708680656302391 1107 46-47 -0.043097355484462696 1107 48-49 0.06648324132257954 1107 50-51 -0.02693481443245105 1107 52-53 -0.08309016486432341 1107 54-55 -0.2010226801468562 1107 56-57 -0.12300724048910183 1107 58-59 -0.24304497571193906 1107 60-61 -0.152367533658051 1107 62-63 -0.16338383282898405 1107 64-65 -0.07121657494321454 1107 66-67 -0.016956376134615425 1107 68-69 -0.19397372385288492 1107 70-71 -0.22807469178398065 1107 72-73 -0.1737406302356348 1107 74-75 -0.1474074657854061 1107 76-77 -0.24287884454477293 1107 78-79 -0.08109245046767555 1107 80-81 -0.11752433202875068 1107 82-83 -0.24928283250723027 1107 84-85 -0.13070249767494246 1107 86-87 -0.1839069210044535 1107 88-89 -0.08783452496198052 1107 90-91 -0.0729641767057565 1107 92-93 -0.1347080154035254 1107 94-95 -0.2573318347464202 1107 96-97 0.0562008210718794 1107 98-99 0.14749523325655645 1107 100-101 -0.44367305017947345 1108 1 0.027526612980686593 1108 2 0.010331759301003274 1108 3 0.027956326329082515 1108 4 -0.01932704757898307 1108 5 0.059895890756571646 1108 6 0.007981045406161513 1108 7 -0.07647909060536051 1108 8 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74-75 -0.34902945067351965 2316 76-77 -0.4163930845912702 2316 78-79 -0.2668230399281093 2316 80-81 -0.11283213983655926 2316 82-83 -0.09557872517408939 2316 84-85 -0.08913572929365543 2316 86-87 -0.41097875089886315 2316 88-89 -0.7277662914058425 2316 90-91 -0.7836602558494157 2316 92-93 -0.8632329112671258 2316 94-95 -0.402877582106683 2316 96-97 -0.5930487308994472 2316 98-99 -0.5997318325188985 2316 100-101 -0.30664741567399645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 5.0 5 23.0 6 59.0 7 147.0 8 264.0 9 484.0 10 944.0 11 1535.0 12 2177.0 13 3233.0 14 3672.0 15 4921.0 16 6599.0 17 9479.0 18 9295.0 19 5903.0 20 5482.0 21 5797.0 22 5855.0 23 6197.0 24 7074.0 25 8003.0 26 9263.0 27 10791.0 28 13047.0 29 16180.0 30 19841.0 31 24254.0 32 30557.0 33 39922.0 34 56299.0 35 89662.0 36 147529.0 37 251467.0 38 210208.0 39 17421.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80095761513832 18.738100886833184 14.576867045608283 21.88407445242021 2 15.802667661853881 22.182937945062147 36.98657571906021 25.027818674023756 3 21.603899206032086 24.230626821402648 30.14577082883529 24.019703143729977 4 13.409138202390988 16.633271231827493 36.70433463300355 33.25325593277797 5 13.592218782269905 37.97476145303846 33.20802311074812 15.22499665394351 6 31.305118342935128 35.557003613741 16.226273528638824 16.911604514685035 7 27.599348569206704 33.65878820976697 20.403031282941562 18.338831938084766 8 25.984448897169088 31.546620580640937 19.690541064661442 22.77838945752853 9 25.269125521569606 18.400086753231022 21.72826504284418 34.60252268235519 10-11 25.934282473600344 25.35275251003084 26.144473438173183 22.56849157819563 12-13 28.070238854701042 23.45224127167732 26.474438570799137 22.00308130282251 14-15 23.560436017614602 25.353598757305836 27.143635222368495 23.942330002711067 16-17 21.684203327569193 28.57269165762967 27.203659739929854 22.539445274871284 18-19 21.388383859600445 28.11195465385168 28.97865655587719 21.52100493067069 20-21 23.164773498841825 25.638315228806764 28.124752709328803 23.072158563022608 22-23 22.180007092662805 25.75022494292165 29.058131503439355 23.011636460976188 24-25 23.82867911698848 25.956195266064082 28.425108539764043 21.7900170771834 26-27 22.679734539297893 25.222625708421138 30.539069285321407 21.558570466959562 28-29 21.23475834633492 28.034091675109153 29.12129137264518 21.60985860591075 30-31 21.82088894598805 26.012336349439042 28.755554752259393 23.411219952313516 32-33 22.169993346965054 26.540626987484284 29.083629919313637 22.20574974623703 34-35 23.652679691560916 25.808274937565706 28.460804161504523 22.07824120936886 36-37 22.86247287612948 25.354326553566864 29.832226554006613 21.950974016297042 38-39 22.14499296435272 26.511980143839896 29.382182223264543 21.96084466854284 40-41 22.024303009369746 26.896434474062573 28.93468047156026 22.144582045007418 42-43 21.955201641466573 27.025623488605 27.26813063338137 23.75104423654706 44-45 22.49572935153067 25.757053958428283 27.917266902217904 23.829949787823143 46-47 23.63015103851189 26.964526465933293 27.77486859844858 21.630453897106236 48-49 23.039749663192264 26.429466744693386 27.988649652543373 22.542133939570977 50-51 20.96459284334342 27.014705559097003 29.339654208095507 22.68104738946407 52-53 22.59927656757938 27.901386538099874 26.664321983601873 22.835014910718865 54-55 21.429025012871328 26.322669264053193 27.76586983302934 24.48243589004614 56-57 22.87950386530584 26.135729728515145 27.327805094407644 23.656961311771376 58-59 24.211588156847036 25.73155064017703 27.102765388917927 22.954095814058004 60-61 22.551769756730508 26.547621141328452 28.979436702837592 21.921172399103444 62-63 21.48060001299406 26.939522414511536 28.42890563136239 23.150971941132017 64-65 21.192233817848578 28.188753827305714 27.371952565833567 23.24705978901214 66-67 22.105031764524853 26.680002637884414 26.58259631331228 24.63236928427845 68-69 21.412196419438338 26.67605877361684 27.516121515757096 24.395623291187718 70-71 22.930989172454286 26.653122872079038 26.74407699153863 23.671810963928046 72-73 22.353904335023785 25.51849221321365 28.59740150626274 23.530201945499822 74-75 21.174724719688392 28.307833289207725 26.806064519784716 23.711377471319167 76-77 21.656102403834858 26.328414440240934 26.991201995754714 25.024281160169497 78-79 21.91383549150855 26.574448618756463 27.443547871043883 24.068168018691104 80-81 22.612942589084472 26.425258731611812 26.932756648178742 24.029042031124977 82-83 21.795611402869035 25.86322868312289 28.155907011096787 24.185252902911287 84-85 21.373241122203844 26.44986825818465 27.59461035782777 24.582280261783737 86-87 21.544798541533815 26.537737030096515 27.24105183266761 24.676412595702065 88-89 21.097504627872002 26.5116979458821 26.262719903626465 26.12807752261943 90-91 21.65017466304475 25.257948603014412 27.054258585658452 26.037618148282377 92-93 22.312872401432994 26.058990243192365 27.112729375835904 24.515407979538743 94-95 21.497558111475946 25.461194048806508 27.463698950657147 25.5775488890604 96-97 20.225421627541415 25.613256440792387 28.60707331918057 25.554248612485626 98-99 21.093885478444836 26.300654131147656 26.541331015957358 26.064129374450147 100-101 20.652150020003255 24.71044130742631 27.582410228379022 27.054998444191412 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1594.0 1 1054.0 2 690.0 3 889.5 4 761.5 5 723.5 6 1237.5 7 2318.5 8 3346.5 9 3529.5 10 2808.5 11 2122.5 12 2035.0 13 2077.5 14 2099.0 15 2085.0 16 2033.5 17 2057.0 18 2115.0 19 2167.0 20 2251.0 21 2475.0 22 2691.5 23 2979.0 24 3364.0 25 3766.0 26 4346.0 27 5008.5 28 5684.5 29 6526.0 30 7677.0 31 9093.5 32 11222.0 33 12767.5 34 13357.0 35 14182.0 36 15678.5 37 17285.5 38 18425.5 39 18993.5 40 19823.5 41 22672.5 42 25617.5 43 27844.0 44 29654.5 45 31609.5 46 34910.0 47 48990.0 48 74663.0 49 72451.5 50 50695.0 51 41130.5 52 36584.0 53 35064.0 54 34080.5 55 31818.0 56 28466.5 57 25109.0 58 23195.0 59 21137.0 60 18045.5 61 15478.0 62 13323.0 63 10779.5 64 8239.5 65 6413.0 66 4795.5 67 4017.5 68 3089.0 69 2091.5 70 1642.0 71 1330.5 72 1088.0 73 848.5 74 692.5 75 589.5 76 374.0 77 198.0 78 127.5 79 85.5 80 47.5 81 25.0 82 14.0 83 6.5 84 4.5 85 4.5 86 2.5 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3476967896419768 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010257983397698109 16-17 0.002246986839495776 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.88475399890386E-4 26-27 0.0013677311196930813 28-29 0.0 30-31 0.0031750900992875094 32-33 0.0 34-35 0.011430324357435035 36-37 0.027208079773894507 38-39 0.022762953634891992 40-41 0.013628463656941772 42-43 0.011528019437413113 44-45 0.0035658704191998186 46-47 0.0012700360397150038 48-49 0.0011723409597369267 50-51 4.8847539989038606E-5 52-53 4.8847539989038606E-5 54-55 0.0 56-57 0.0 58-59 0.004689363838947707 60-61 0.01162571451739119 62-63 0.004738211378936746 64-65 0.002637767159408085 66-67 0.004445126139002513 68-69 0.003761260579155973 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2173715529512218 78-79 4.396278599013475E-4 80-81 0.0041031933590792435 82-83 8.304081798136565E-4 84-85 0.0 86-87 0.11225164689481074 88-89 0.4128594079873544 90-91 0.3964954820910264 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0021004442195286604 100-101 9.281032597917336E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1023593.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.41087999316544 #Duplication Level Percentage of deduplicated Percentage of total 1 64.72978738376457 22.921387330296014 2 15.256708673186617 10.805069598337951 3 6.892813793565435 7.322418061775431 4 3.772876697883572 5.344035359110618 5 2.3671178816861707 4.191086361903248 6 1.5297582751719492 3.250205202039939 7 1.059841346222931 2.6270940306026582 8 0.7623871752630278 2.159744061725397 9 0.5719939899407771 1.8229329481144265 >10 2.781940622084337 17.34544292855175 >50 0.16773477032347286 4.122170262262787 >100 0.09379740441227861 5.589998044851629 >500 0.005241619654192345 1.3241834165261295 >1k 0.006345118528759153 6.079423266488683 >5k 0.0013793735932085118 3.3647251505281797 >10k+ 2.758747186417023E-4 1.7300839768851166 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17710 1.7301798664117476 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9748 0.9523316396262967 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7271 0.7103409265205995 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6697 0.6542639506131832 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5602 0.5472878380371886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5125 0.5006872848876458 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4755 0.4645401052957572 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4592 0.4486158072593306 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4375 0.42741597490408784 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4367 0.4266344142642632 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4173 0.40768156874851624 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3860 0.3771030087153781 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3844 0.37553988743572886 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3615 0.35316771412074915 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3096 0.30246396761212707 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2546 0.2487316736241846 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2417 0.23612900830701267 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2379 0.2324165952678457 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2340 0.22860648714870072 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2291 0.2238194282297749 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2143 0.20936055639301948 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1700 0.1660816359627313 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1653 0.16148996720376166 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1594 0.15572595748505508 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1502 0.146738010127072 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1380 0.1348192103697466 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1296 0.1266128236515881 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1207 0.1179179615335392 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1107 0.10814845353573149 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.884753998903861E-5 0.0 12-13 0.0 0.0 0.0 9.769507997807723E-5 0.0 14-15 0.0 0.0 0.0 9.769507997807723E-5 0.0 16-17 0.0 0.0 0.0 1.9539015995615445E-4 0.0 18-19 0.0 0.0 0.0 2.9308523993423167E-4 0.0 20-21 0.0 0.0 0.0 4.3962785990134755E-4 0.0 22-23 0.0 0.0 0.0 0.001123493419747888 0.0 24-25 0.0 0.0 0.0 0.003614717959188857 0.0 26-27 0.0 0.0 0.0 0.006838655598465406 0.0 28-29 0.0 0.0 0.0 0.01968555861558256 0.0 30-31 0.0 0.0 0.0 0.04884753998903861 0.0 32-33 0.0 0.0 0.0 0.10023515205750723 0.0 34-35 0.0 0.0 0.0 0.17482534562076918 0.0 36-37 0.0 0.0 0.0 0.2764282287979695 0.0 38-39 0.0 0.0 0.0 0.42297084876508534 0.0 40-41 0.0 0.0 0.0 0.5722000834315983 0.0 42-43 0.0 0.0 0.0 0.7298310949762259 0.0 44-45 0.0 0.0 0.0 0.9293244482914595 0.0 46-47 0.0 0.0 0.0 1.1684820040777928 0.0 48-49 0.0 0.0 0.0 1.3916175667477209 0.0 50-51 0.0 0.0 0.0 1.6289677635544595 0.0 52-53 0.0 0.0 0.0 1.8577207933231275 0.0 54-55 0.0 0.0 0.0 2.134539802441009 0.0 56-57 0.0 0.0 0.0 2.4804292331033917 0.0 58-59 0.0 0.0 0.0 2.8155722049681855 0.0 60-61 0.0 0.0 0.0 3.1859342531650765 0.0 62-63 0.0 0.0 0.0 3.549946121163392 0.0 64-65 0.0 0.0 0.0 3.931054628157871 0.0 66-67 0.0 0.0 0.0 4.38128240423684 0.0 68-69 0.0 0.0 0.0 4.899554803520539 0.0 70-71 0.0 0.0 0.0 5.444791044878189 0.0 72-73 0.0 0.0 0.0 5.97517763407917 0.0 74-75 0.0 0.0 0.0 6.556072579628817 0.0 76-77 0.0 0.0 0.0 7.152012567495088 0.0 78-79 0.0 0.0 0.0 7.90748862096556 0.0 80-81 0.0 0.0 0.0 8.701651926107349 0.0 82-83 0.0 0.0 0.0 9.446381520780232 0.0 84-85 0.0 0.0 0.0 10.224229747565683 0.0 86-87 0.0 0.0 0.0 11.022789331306486 0.0 88-89 0.0 0.0 0.0 11.8863649907727 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4910 0.0 23.075243 1 TACATGG 4940 0.0 21.994514 2 AGTACTT 4630 0.0 21.779476 12-13 GTACCGT 110 0.0028959645 21.566673 6 ACCGTGC 135 3.824447E-4 21.087416 8 ACATGGG 5020 0.0 20.793365 3 GAGTACT 4640 0.0 20.454153 12-13 GTGTAAG 210 8.425559E-7 20.402063 1 TACCGTC 120 0.004794195 19.769451 7 GTCTTAG 245 1.7152524E-7 19.430534 1 CATGGGA 2795 0.0 19.182379 4 GTACTTT 4905 0.0 19.05885 14-15 GGATAGG 150 7.6066435E-4 19.041925 1 TAAGGTG 200 1.2970097E-5 18.978672 3 TAAGGTA 150 7.778798E-4 18.978672 4 AGAGTAC 8205 0.0 18.767464 10-11 TACTTTT 5340 0.0 18.750406 14-15 TTTGCGT 255 2.705856E-7 18.60654 3 GTCTATA 130 0.0076117143 18.248724 2 TACATAC 235 2.5541249E-6 18.171068 3 >>END_MODULE