##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939202_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1009656 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.332334973495925 34.0 31.0 34.0 31.0 34.0 2 32.40371076881631 34.0 31.0 34.0 31.0 34.0 3 32.47503902319206 34.0 31.0 34.0 31.0 34.0 4 35.889570309095376 37.0 35.0 37.0 35.0 37.0 5 35.93331293034459 37.0 35.0 37.0 35.0 37.0 6 36.042197540548464 37.0 37.0 37.0 35.0 37.0 7 35.98902002266119 37.0 37.0 37.0 35.0 37.0 8 35.999183880450374 37.0 37.0 37.0 35.0 37.0 9 37.70291960826262 39.0 38.0 39.0 35.0 39.0 10-11 37.61953823876647 39.0 38.0 39.0 35.0 39.0 12-13 37.55319633617786 39.0 38.0 39.0 35.0 39.0 14-15 38.86097740220431 40.5 38.0 41.0 36.0 41.0 16-17 38.81852482429659 40.0 38.0 41.0 35.0 41.0 18-19 38.75654133685136 40.0 38.5 41.0 35.0 41.0 20-21 38.7420264921914 40.0 38.5 41.0 35.0 41.0 22-23 38.764936275325454 40.5 39.0 41.0 35.0 41.0 24-25 38.70260563994073 40.5 38.5 41.0 35.0 41.0 26-27 38.580249609768074 40.0 38.0 41.0 35.0 41.0 28-29 38.46715168334562 40.0 38.0 41.0 34.0 41.0 30-31 38.26935411664963 40.0 38.0 41.0 34.0 41.0 32-33 38.042977509171436 40.0 38.0 41.0 34.0 41.0 34-35 37.694503375407066 40.0 37.0 41.0 33.0 41.0 36-37 37.42827259977656 40.0 37.0 41.0 33.0 41.0 38-39 37.24018626145935 40.0 37.0 41.0 32.5 41.0 40-41 37.080316464221475 40.0 36.5 41.0 32.0 41.0 42-43 36.84586482920916 40.0 36.0 41.0 31.0 41.0 44-45 36.52496097680795 40.0 35.5 41.0 30.5 41.0 46-47 36.31864565753088 40.0 36.0 41.0 30.0 41.0 48-49 36.1267094931343 40.0 35.0 41.0 30.0 41.0 50-51 34.938821737304586 38.0 34.0 40.0 27.0 40.5 52-53 35.085965913142694 38.5 35.0 39.5 27.5 40.5 54-55 35.596867646010125 39.0 35.0 41.0 28.0 41.0 56-57 35.43346793363284 39.0 35.0 41.0 28.0 41.0 58-59 35.22431848074988 39.0 35.0 41.0 27.5 41.0 60-61 34.866429754292554 38.0 35.0 40.5 26.5 41.0 62-63 34.51823343792341 37.5 35.0 40.0 26.0 41.0 64-65 34.12745727257601 37.0 34.0 40.0 25.5 41.0 66-67 33.668928823282386 36.5 34.0 39.0 24.0 41.0 68-69 33.22121346280318 36.0 34.0 39.0 22.5 41.0 70-71 32.7217047192311 35.0 33.0 38.5 21.5 40.0 72-73 32.27832598429564 35.0 33.0 37.0 20.5 39.5 74-75 31.86741721933015 35.0 33.0 37.0 19.0 39.0 76-77 31.44673136196883 35.0 32.0 36.0 17.5 39.0 78-79 31.021584084084083 35.0 32.0 36.0 11.5 37.5 80-81 30.556765868771144 35.0 31.0 35.5 7.0 37.0 82-83 30.22502416664686 35.0 31.0 35.0 2.0 37.0 84-85 29.876967997020767 34.0 31.0 35.0 2.0 36.0 86-87 29.548559113202913 34.0 31.0 35.0 2.0 36.0 88-89 29.137891519487823 34.0 30.0 35.0 2.0 35.5 90-91 28.724501711474005 34.0 29.0 35.0 2.0 35.0 92-93 28.32789781866299 34.0 29.0 35.0 2.0 35.0 94-95 27.858471103029153 34.0 29.0 35.0 2.0 35.0 96-97 27.293521258725743 34.0 27.0 35.0 2.0 35.0 98-99 26.66163079306219 34.0 25.5 35.0 2.0 35.0 100-101 23.886658426236263 30.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1794005108849248 1101 2 0.34592043008320417 1101 3 0.2645809188435777 1101 4 0.31112033995380983 1101 5 0.1836307425404513 1101 6 0.08444642339937047 1101 7 0.0020364830077852503 1101 8 0.12595177472209684 1101 9 0.14010686529628913 1101 10-11 0.2055494882766311 1101 12-13 0.15938694648417595 1101 14-15 0.3035096396942407 1101 16-17 0.34885100970930694 1101 18-19 0.39195204492417446 1101 20-21 0.35819626872777377 1101 22-23 0.40438315710144934 1101 24-25 0.49391588234981754 1101 26-27 0.23442512272017524 1101 28-29 0.18172100731541008 1101 30-31 0.2119378472419342 1101 32-33 0.265200557096847 1101 34-35 0.26049894107193694 1101 36-37 0.1860579292704827 1101 38-39 0.14434139739485374 1101 40-41 0.27001050769622026 1101 42-43 0.3347064611248456 1101 44-45 0.32891315641456487 1101 46-47 0.3654224085304847 1101 48-49 0.4810933784470137 1101 50-51 0.45603561827845596 1101 52-53 0.546480920135842 1101 54-55 0.3794707984689296 1101 56-57 0.3929012632360198 1101 58-59 0.5361721827884267 1101 60-61 0.3435191698347353 1101 62-63 0.35642390176301575 1101 64-65 0.33310476627296737 1101 66-67 0.37395236070204874 1101 68-69 0.136856323601954 1101 70-71 0.21915806827357898 1101 72-73 0.1158060920866788 1101 74-75 0.1365363547549343 1101 76-77 0.11630243769560522 1101 78-79 0.19513939359330124 1101 80-81 -0.17663851986672796 1101 82-83 -0.16186146306189642 1101 84-85 -0.14649291239708262 1101 86-87 -0.18209290264572786 1101 88-89 -0.1631997680616415 1101 90-91 -0.07484151318813659 1101 92-93 -0.4358534796042015 1101 94-95 -0.1298564184594504 1101 96-97 -0.5165693378891554 1101 98-99 -0.47389634132597536 1101 100-101 -0.6201731105813657 1102 1 0.23440568126991224 1102 2 0.2569021495036452 1102 3 0.35713229583001294 1102 4 0.19897795824865483 1102 5 0.35199363225078883 1102 6 0.25829170408590585 1102 7 0.38018774351198914 1102 8 0.33213573345410197 1102 9 0.32718565720909254 1102 10-11 0.38197253274194054 1102 12-13 0.3158364074566862 1102 14-15 0.3505735147492146 1102 16-17 0.3608966695574054 1102 18-19 0.43291471712176843 1102 20-21 0.6258275786775016 1102 22-23 0.5120898536414487 1102 24-25 0.5224154009691446 1102 26-27 0.6536980299028485 1102 28-29 0.5212815840213452 1102 30-31 0.493920689883943 1102 32-33 0.591566251889617 1102 34-35 0.4244699750300711 1102 36-37 0.24580187635426398 1102 38-39 0.2097564247919479 1102 40-41 0.4145955694436978 1102 42-43 0.1913330226405492 1102 44-45 0.22590215003180703 1102 46-47 0.39165096926170406 1102 48-49 0.2812423984510559 1102 50-51 0.3361614309797716 1102 52-53 0.3316484524953225 1102 54-55 0.42457497875491157 1102 56-57 0.2178926459277193 1102 58-59 0.1676767648192623 1102 60-61 0.0739400510585071 1102 62-63 0.08115587375973377 1102 64-65 0.12987074989413827 1102 66-67 0.05919031727081148 1102 68-69 0.20816232124853684 1102 70-71 -0.08531817767034511 1102 72-73 0.1099842088779539 1102 74-75 -0.01974298866560531 1102 76-77 -0.098318311594209 1102 78-79 -0.07201350717096489 1102 80-81 -0.05347638372514396 1102 82-83 -0.2523870297242041 1102 84-85 -0.24173571248741865 1102 86-87 -0.2773126737224736 1102 88-89 -0.1937555547327463 1102 90-91 -0.3227793021651131 1102 92-93 -0.5118596246364753 1102 94-95 -0.453864982281015 1102 96-97 -0.6127719278360253 1102 98-99 -0.6635187249328816 1102 100-101 -1.0745273770404253 1103 1 0.09506323100309544 1103 2 0.1264513677923631 1103 3 0.33356762520651984 1103 4 0.24138932839111504 1103 5 0.27861043281716746 1103 6 0.15510637400981864 1103 7 0.2875842685555696 1103 8 0.23959102326233506 1103 9 0.23995915821788572 1103 10-11 0.31617978207523834 1103 12-13 0.29766760147275306 1103 14-15 0.3692537141016814 1103 16-17 0.31221705785258536 1103 18-19 0.3473644130840654 1103 20-21 0.44546172604274403 1103 22-23 0.39848497112900816 1103 24-25 0.30449886372487356 1103 26-27 0.3469060823546002 1103 28-29 0.37135727863485357 1103 30-31 0.41448262095007493 1103 32-33 0.4391227570091445 1103 34-35 0.3568576154009051 1103 36-37 0.27700596638188557 1103 38-39 0.2637836300796579 1103 40-41 0.24655912409632919 1103 42-43 0.18743356075788853 1103 44-45 0.2687542559091298 1103 46-47 0.21938136211814907 1103 48-49 0.3826647855877283 1103 50-51 0.14110508676376554 1103 52-53 0.17886915895972777 1103 54-55 0.16751691077246988 1103 56-57 0.310658423567439 1103 58-59 0.2518908700417697 1103 60-61 0.15340400447204416 1103 62-63 0.182068966269469 1103 64-65 0.23859675777981693 1103 66-67 0.166617204109464 1103 68-69 0.09873953115347689 1103 70-71 0.175734495147033 1103 72-73 0.1855039272548069 1103 74-75 0.024227046901067695 1103 76-77 0.03173580795570885 1103 78-79 0.11929238581559787 1103 80-81 0.040363949610988215 1103 82-83 -0.24323161930828974 1103 84-85 -0.23818813961211305 1103 86-87 0.004228614121338126 1103 88-89 -0.028474757099008485 1103 90-91 0.05154035576798677 1103 92-93 -0.1504528241540406 1103 94-95 -0.3277564969288349 1103 96-97 -0.25639860927783076 1103 98-99 -0.36714912814311873 1103 100-101 -0.4243155440554851 1104 1 -5.893313912963549E-4 1104 2 0.18524282060861452 1104 3 0.18933981243304743 1104 4 0.16330732020764316 1104 5 0.25161075080057316 1104 6 0.24896666629175712 1104 7 0.18467458898297906 1104 8 0.20456948711340317 1104 9 0.28117085895608085 1104 10-11 0.2901209104695823 1104 12-13 0.18932920931248987 1104 14-15 0.22949080883708461 1104 16-17 0.236458168113991 1104 18-19 0.24388577972945313 1104 20-21 0.29485979133708184 1104 22-23 0.30674413023371017 1104 24-25 0.31508692279094674 1104 26-27 0.18419252758918248 1104 28-29 0.3016742371367016 1104 30-31 0.4325216093949251 1104 32-33 0.38051723250734426 1104 34-35 0.3246000383293435 1104 36-37 0.18243086842459633 1104 38-39 0.12164767509138841 1104 40-41 0.25116988349851255 1104 42-43 0.2578642574569088 1104 44-45 0.24831133918662118 1104 46-47 0.3042284015875154 1104 48-49 0.17662467763104672 1104 50-51 0.3530114629880643 1104 52-53 0.4359068413074425 1104 54-55 0.21212788041162156 1104 56-57 0.22905494624806977 1104 58-59 0.22043022245936328 1104 60-61 0.24236051958413896 1104 62-63 0.1392620925719541 1104 64-65 0.12574418382239116 1104 66-67 0.2877721671471818 1104 68-69 0.3573493917926527 1104 70-71 0.33378407427890977 1104 72-73 0.49607122542075643 1104 74-75 0.4520369296151223 1104 76-77 0.30669487744690827 1104 78-79 0.41892604790698584 1104 80-81 0.2441926275095625 1104 82-83 0.15555833556960152 1104 84-85 -0.08546215458624218 1104 86-87 0.010843851570847107 1104 88-89 0.196313889053787 1104 90-91 0.22213516869651073 1104 92-93 0.15441770929596998 1104 94-95 -0.20715209193886253 1104 96-97 -0.315297808537796 1104 98-99 -0.07826974371694462 1104 100-101 -0.26340608523170417 1105 1 0.053730073985846616 1105 2 0.11709494742370197 1105 3 0.15466643740655428 1105 4 0.13903006362917836 1105 5 0.1869416270215538 1105 6 0.045958940057047926 1105 7 0.24786518156173543 1105 8 0.1951114020307685 1105 9 0.08890035421136133 1105 10-11 0.028036520072134863 1105 12-13 0.11415172913596905 1105 14-15 0.09388040368943962 1105 16-17 0.09403886970776654 1105 18-19 0.29135553505071243 1105 20-21 0.20897518699779738 1105 22-23 0.19982212118695486 1105 24-25 0.2395821341807789 1105 26-27 0.1815728060647004 1105 28-29 0.19051791036500276 1105 30-31 0.21872963459642136 1105 32-33 -0.031373469048816105 1105 34-35 0.0926900995648694 1105 36-37 0.06729354309882751 1105 38-39 0.02483774096692315 1105 40-41 0.2096092367571103 1105 42-43 0.19369926390902492 1105 44-45 0.12624159615509 1105 46-47 -0.05143171431227955 1105 48-49 0.10960043799612862 1105 50-51 -0.18949599550583685 1105 52-53 0.005081785784142312 1105 54-55 -0.0642207620108124 1105 56-57 0.07166975187576696 1105 58-59 -0.09691160668061372 1105 60-61 -0.17414071628736139 1105 62-63 -0.17228098485467314 1105 64-65 -0.20868602988117146 1105 66-67 -0.2116942323221025 1105 68-69 -0.09258934760587323 1105 70-71 -0.19146319407513346 1105 72-73 -0.14133812205665208 1105 74-75 -0.12484042252235028 1105 76-77 -0.17232477736182972 1105 78-79 -0.28334742894880094 1105 80-81 -0.2886937187125298 1105 82-83 -0.2754592174424495 1105 84-85 -0.42338679338696394 1105 86-87 -0.4664063312077147 1105 88-89 -0.2514854320006741 1105 90-91 -0.12160138939822573 1105 92-93 -0.16432928880915298 1105 94-95 -0.0821955136036685 1105 96-97 -0.23810192635378158 1105 98-99 -0.2616803477577605 1105 100-101 -0.7880225637691787 1106 1 0.0731106288978225 1106 2 0.15780296784625136 1106 3 0.15506673678196847 1106 4 0.1468807835008903 1106 5 0.13564144251993326 1106 6 0.04857422930973598 1106 7 0.12853593775151495 1106 8 0.09741894597575396 1106 9 0.1398099435648561 1106 10-11 0.17803294163832817 1106 12-13 0.19945855860722617 1106 14-15 0.1885590906589698 1106 16-17 0.22055045473560142 1106 18-19 0.355868631511953 1106 20-21 0.36544250861894767 1106 22-23 0.346758072073861 1106 24-25 0.2698884359846758 1106 26-27 0.11997461687351318 1106 28-29 0.13965259654848694 1106 30-31 0.2092419328849573 1106 32-33 0.22651030584707144 1106 34-35 0.2921926366137839 1106 36-37 0.18758775100946679 1106 38-39 0.012236195326117638 1106 40-41 0.10296099194857078 1106 42-43 0.15458091721822598 1106 44-45 0.01782960803792122 1106 46-47 0.1528149768494771 1106 48-49 0.2718951481005334 1106 50-51 0.12232933325518758 1106 52-53 0.060193305551052845 1106 54-55 0.03536280533592162 1106 56-57 0.05051271685685066 1106 58-59 0.2913399705595694 1106 60-61 0.37667183079330613 1106 62-63 0.3961398826304574 1106 64-65 0.22265595557946938 1106 66-67 0.22119557739012663 1106 68-69 0.15861731240530474 1106 70-71 0.16912405723888213 1106 72-73 0.2748852838117344 1106 74-75 0.3541286974134046 1106 76-77 0.4835251127984499 1106 78-79 0.41635283173100746 1106 80-81 0.2290213481920489 1106 82-83 0.23711564157332177 1106 84-85 0.5162438152015234 1106 86-87 0.3850316533213203 1106 88-89 0.20675121285978904 1106 90-91 0.12611781393323085 1106 92-93 0.06130551928368888 1106 94-95 0.18798412377361728 1106 96-97 0.27957432364166834 1106 98-99 0.16849652391474734 1106 100-101 0.05603171682612995 1107 1 -0.020113829379447168 1107 2 0.11723911885408711 1107 3 0.012080892760884865 1107 4 -0.07195486166540377 1107 5 0.1261692666773726 1107 6 0.11187107050054124 1107 7 0.22964449143771049 1107 8 0.14773995195920975 1107 9 -0.022550022099508737 1107 10-11 0.07734783776384546 1107 12-13 0.08244104720510848 1107 14-15 0.11115108613604008 1107 16-17 0.021656020716207536 1107 18-19 0.0624552583118998 1107 20-21 0.18193073648435387 1107 22-23 0.16996691384382956 1107 24-25 0.20554302504300637 1107 26-27 0.2507522362007535 1107 28-29 0.2724653030305504 1107 30-31 0.3061309269819432 1107 32-33 0.21051688799231272 1107 34-35 0.2086713268036604 1107 36-37 0.11204763703999276 1107 38-39 0.05429676990787158 1107 40-41 0.1879008873558945 1107 42-43 0.18652351190264227 1107 44-45 0.14680770930504394 1107 46-47 0.2289049435595345 1107 48-49 0.3304886512198095 1107 50-51 0.1946622601839536 1107 52-53 0.13591325641478136 1107 54-55 0.11842216989690257 1107 56-57 0.12909569405950805 1107 58-59 0.09617672117693843 1107 60-61 0.03425146751128949 1107 62-63 -0.05970067960976877 1107 64-65 0.07477199806464085 1107 66-67 -0.005278252032560715 1107 68-69 0.16427901450118298 1107 70-71 0.19923610768973532 1107 72-73 0.2671528719174532 1107 74-75 0.12195352133987214 1107 76-77 0.29276815460449157 1107 78-79 0.26127597300466476 1107 80-81 0.2416444043160979 1107 82-83 0.4203935682895015 1107 84-85 0.6047166724707829 1107 86-87 0.3687670437282584 1107 88-89 0.3902514938190258 1107 90-91 0.4045036025668516 1107 92-93 0.37023083009125557 1107 94-95 0.41603281877828735 1107 96-97 0.3992037792012688 1107 98-99 0.26938214879911726 1107 100-101 0.1370967879047953 1108 1 -0.04508459585359503 1108 2 0.1062026291891911 1108 3 0.037928665010596774 1108 4 0.06500031655906469 1108 5 0.10407294498853759 1108 6 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-0.7960342981661981 2316 56-57 -0.9123342049602385 2316 58-59 -0.7056375793685916 2316 60-61 -0.7915507947262981 2316 62-63 -0.7360233964723406 2316 64-65 -1.0081729546725597 2316 66-67 -0.8674244614820097 2316 68-69 -0.9561577241127779 2316 70-71 -0.8372081587550291 2316 72-73 -1.0609096837344474 2316 74-75 -0.6615400765044335 2316 76-77 -0.6102114821465641 2316 78-79 -0.3053620495399443 2316 80-81 -0.4067506823744935 2316 82-83 -0.5011991745083186 2316 84-85 -0.3603520150970212 2316 86-87 -0.4089854166203679 2316 88-89 -0.47696433640039615 2316 90-91 -0.5824755053696435 2316 92-93 -0.6567177154742865 2316 94-95 -0.5639029458059106 2316 96-97 -0.5824086386088929 2316 98-99 -1.0823724656911793 2316 100-101 -0.9627259036835518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3320.0 3 574.0 4 592.0 5 524.0 6 709.0 7 731.0 8 964.0 9 1252.0 10 1608.0 11 2159.0 12 3215.0 13 5956.0 14 5010.0 15 5071.0 16 6827.0 17 10056.0 18 8393.0 19 5364.0 20 5479.0 21 5947.0 22 5981.0 23 6453.0 24 7322.0 25 8153.0 26 9451.0 27 11030.0 28 12580.0 29 15385.0 30 18521.0 31 22933.0 32 29316.0 33 38864.0 34 54587.0 35 88335.0 36 143751.0 37 226912.0 38 205906.0 39 30420.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54089903907344 18.39193951253659 14.213141943489882 21.854019504900087 2 15.706981762218678 21.946321901996715 37.31179149115083 25.034904844633775 3 21.668405332614068 24.126865760444886 30.032942100440685 24.17178680650036 4 13.224975627946092 16.370780510687506 37.13031585983515 33.27392800153125 5 13.65937691843092 37.65068515567121 33.24718266487225 15.442755261025617 6 31.13206892621208 35.30510801314296 16.55666291672655 17.006160143918407 7 27.545796986518635 33.59218873909596 20.712529738302933 18.149484536082475 8 25.821272343930744 31.38791333731814 19.95686096085469 22.833953357896416 9 25.511731411635264 18.354430379746837 21.952955403784582 34.180882804833324 10-11 25.9437183738 25.17423217409836 26.303333977628103 22.578715474473537 12-13 28.09004078569839 23.482580209643146 26.544307919384185 21.883071085274274 14-15 23.729859363082326 25.24942398682889 27.297101869459482 23.723614780629305 16-17 21.71414977685375 28.456710122984436 27.281828386780226 22.54731171338159 18-19 21.332786795053597 28.168414416120168 29.05176434551463 21.447034443311605 20-21 23.220355616619234 25.662357513954937 28.158285776029135 22.959001093396697 22-23 22.256843361704934 25.71556459252114 29.061367814798604 22.966224230975325 24-25 23.80528630692229 25.84183713938971 28.46823416963457 21.88464238405343 26-27 22.712807859549763 25.124125420836613 30.530763521924655 21.632303197688973 28-29 21.215080537900874 28.09945623966104 29.058782530069415 21.626680692368666 30-31 21.92448082924566 26.081366161606145 28.626760144499386 23.367392864648803 32-33 22.189381228422796 26.57691707167163 29.0219161175039 22.211785582401678 34-35 23.678276407638858 25.87738913005454 28.344185787657615 22.100148674648985 36-37 22.832168058474345 25.26338796508308 29.832430438243918 22.07201353819866 38-39 22.160617936670153 26.546695943359456 29.29977365485925 21.99291246511114 40-41 22.04750349846625 26.9151987706101 28.835622846427782 22.201674884495873 42-43 21.974528158655176 27.002491431814164 27.193642244329563 23.829338165201094 44-45 22.435557245995135 25.812497946414613 27.84925637806283 23.902688429527423 46-47 23.568066796832607 26.977455272467317 27.80763651244226 21.646841418257814 48-49 23.115939647936337 26.407562627762225 27.88260960045086 22.593888123850576 50-51 21.02243308905528 27.052134777760077 29.3431874303011 22.582244702883543 52-53 22.56751166079955 27.956794926749417 26.55476082728077 22.920932585170263 54-55 21.41951465508272 26.3944817616068 27.69911940568222 24.486884177628255 56-57 22.83264869793196 26.090929269382713 27.415641538374253 23.660780494311073 58-59 24.305546570752092 25.64653263440962 27.091620777819685 22.9563000170186 60-61 22.60850102955366 26.296292612082084 29.118960697908065 21.976245660456186 62-63 21.61362270955976 26.632793395110244 28.712176906877207 23.041406988452792 64-65 21.3500879502878 27.964407641244478 27.632870156697393 23.052634251770332 66-67 22.172623641543836 26.484579848293073 26.812063260015957 24.530733250147136 68-69 21.471922137048 26.47236784507886 27.79241061312991 24.26329940474323 70-71 23.01318278673545 26.41278112331306 27.092113184828413 23.481922905123078 72-73 22.39958813046983 25.34511250979012 28.93463114723489 23.32066821250516 74-75 21.221266478284143 28.14738650367844 27.29598048124681 23.33536653679061 76-77 21.685567828879478 26.151406100111362 27.39170930857141 24.771316762437753 78-79 22.006941665721072 26.432178910634264 27.90427190148289 23.65660752216177 80-81 22.86735454162214 26.20254103284768 27.336932474543136 23.593171950987042 82-83 22.012883545827382 25.56330046159153 28.63853644671587 23.785279545865215 84-85 21.620144489364392 26.30748738430846 28.12729280051926 23.94507532580789 86-87 21.822661692347133 26.367195374778806 27.80732558978788 24.002817343086186 88-89 21.4307653631424 26.27926831393106 26.893968244486093 25.395998078440453 90-91 21.85625701422712 25.117181781880067 27.678202445905185 25.348358757987633 92-93 22.803749579252834 25.735635343160396 27.83120519914038 23.629409878446392 94-95 22.01294061705682 25.17430498330765 28.12933646351209 24.68341793612344 96-97 20.834849891828508 25.21827239299238 29.2568406953846 24.690037019794513 98-99 21.763424477124428 26.016535838853994 27.311519569400982 24.9085201146206 100-101 21.40386614257894 24.26733252888257 28.465410608395757 25.863390720142732 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2483.0 1 1526.0 2 755.0 3 952.5 4 797.5 5 742.0 6 1279.0 7 2335.5 8 3450.5 9 3618.5 10 2821.5 11 2257.0 12 2265.5 13 2401.5 14 2462.0 15 2476.0 16 2531.5 17 2571.0 18 2548.5 19 2513.5 20 2591.5 21 2774.5 22 2918.5 23 3181.5 24 3574.5 25 4056.0 26 4592.5 27 5152.5 28 5827.5 29 6632.0 30 7650.0 31 9140.0 32 11170.5 33 12404.5 34 13019.0 35 13896.0 36 15306.5 37 16805.0 38 17911.0 39 18579.5 40 19546.0 41 22413.5 42 25254.0 43 27230.0 44 28855.0 45 30886.0 46 34882.5 47 47908.5 48 73774.0 49 73807.0 50 49477.0 51 37349.5 52 33828.0 53 33510.0 54 33107.0 55 30968.0 56 27647.0 57 24436.5 58 22582.0 59 20405.5 60 17443.5 61 15126.5 62 13000.0 63 10486.5 64 8092.5 65 6298.0 66 4845.5 67 4125.0 68 3203.0 69 2237.0 70 1805.0 71 1546.0 72 1272.0 73 965.0 74 703.5 75 598.5 76 442.0 77 263.5 78 184.5 79 129.0 80 75.0 81 50.5 82 31.0 83 29.5 84 29.5 85 15.5 86 14.0 87 10.0 88 7.5 89 7.0 90 4.0 91 3.0 92 5.0 93 5.5 94 3.0 95 2.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3922127932682022 2 0.11885236159642491 3 0.12083323428969868 4 0.13162899046804058 5 0.13321368862265962 6 0.07438176963242926 7 0.08478135127211645 8 0.08171099859754213 9 0.08181004223220582 10-11 0.07933395136561364 12-13 0.08384043674281141 14-15 0.07740260048967174 16-17 0.07809590593231754 18-19 0.08740600759070416 20-21 0.08646509306139913 22-23 0.0950323674598081 24-25 0.10775947451409193 26-27 0.10889847631272434 28-29 0.1021635091555936 30-31 0.09230866750655668 32-33 0.09146679661191534 34-35 0.33991775416577524 36-37 0.5334985381159524 38-39 0.5166611202231255 40-41 0.488285118891979 42-43 0.5163639893191344 44-45 0.5264169182374987 46-47 0.5246341328135524 48-49 0.5297844018160641 50-51 0.5284968345654362 52-53 0.5278530509401221 54-55 0.5227027819376104 56-57 0.4937325187984819 58-59 0.48263963171614893 60-61 0.4324245089416593 62-63 0.5078462367380574 64-65 0.5343899308279255 66-67 0.5430562488609982 68-69 0.5581604031472106 70-71 0.5528120468753714 72-73 0.5402335052730831 74-75 0.544096207024967 76-77 0.5214152146869825 78-79 0.4950200860491098 80-81 0.501606487754245 82-83 0.5146802475298518 84-85 0.5103718494219813 86-87 0.5114118075859501 88-89 0.5213161710523188 90-91 0.5394906780131055 92-93 0.543749554303644 94-95 0.5401344616384194 96-97 0.5411248979850564 98-99 0.5421153343316932 100-101 0.5193848201763769 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1009656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.92530648575328 #Duplication Level Percentage of deduplicated Percentage of total 1 66.26019492484096 24.466780054055068 2 14.696631105644917 10.853552157679875 3 6.6610536678179555 7.378843446066872 4 3.5926227679614438 5.306347871786865 5 2.2281979482183036 4.113844607444374 6 1.5008066548774646 3.3250647424325126 7 0.9663782505278261 2.4978669157334235 8 0.7122404824159638 2.103975848381619 9 0.5401245917158939 1.794983948064157 >10 2.5840544458287704 16.71038095666914 >50 0.16132915424259914 4.098506998309805 >100 0.08428670155603887 5.198850055480549 >500 0.004294863769515918 1.0683953540383708 >1k 0.006442295654273878 6.44684763126113 >5k 0.0010737159423789796 2.9491636853193883 >10k+ 2.684289855947449E-4 1.686595727276827 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17016 1.6853264874373053 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9927 0.9832061613064251 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7354 0.7283668893167574 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6741 0.667653141267917 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5732 0.5677181138922563 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4934 0.4886812934306338 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4651 0.46065194482081023 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4289 0.4247981490725554 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4114 0.4074655130064101 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4061 0.40221620036923467 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3906 0.3868644369963631 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3655 0.36200448469577756 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3609 0.357448477501248 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3498 0.3464546340535786 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2761 0.27345947530644105 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2650 0.2624656318587717 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2568 0.25434405381634934 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2432 0.2408741195020878 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2349 0.23265349782500178 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2246 0.22245200345464197 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2179 0.21581607993217491 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1756 0.17392062246943513 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1580 0.15648894276862615 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1573 0.15579563732598034 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1393 0.13796778308651658 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1303 0.12905385596678473 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1246 0.12340836879095454 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1216 0.1204370597510439 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1073 0.10627381999413661 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.9521817331843714E-5 0.0 20-21 0.0 0.0 0.0 1.9808726932737486E-4 0.0 22-23 0.0 0.0 0.0 0.0012875672506279367 0.0 24-25 0.0 0.0 0.0 0.0032684399439016857 0.0 26-27 0.0 0.0 0.0 0.006883532609126277 0.0 28-29 0.0 0.0 0.0 0.020601076010046987 0.0 30-31 0.0 0.0 0.0 0.051007471851799026 0.0 32-33 0.0 4.9521817331843714E-5 0.0 0.104887209108845 0.0 34-35 0.0 9.904363466368743E-5 0.0 0.17758523695199158 0.0 36-37 0.0 9.904363466368743E-5 0.0 0.28088774790621757 0.0 38-39 0.0 9.904363466368743E-5 0.0 0.4247981490725554 0.0 40-41 0.0 9.904363466368743E-5 0.0 0.5887648862582899 0.0 42-43 0.0 9.904363466368743E-5 0.0 0.7475318326241809 0.0 44-45 0.0 9.904363466368743E-5 0.0 0.9425487492769815 0.0 46-47 0.0 9.904363466368743E-5 0.0 1.1837199996830603 0.0 48-49 0.0 9.904363466368743E-5 0.0 1.4036463904537784 0.0 50-51 0.0 9.904363466368743E-5 0.0 1.6504136062183554 0.0 52-53 0.0 9.904363466368743E-5 0.0 1.8801453168207787 0.0 54-55 0.0 9.904363466368743E-5 0.0 2.1476621740473982 0.0 56-57 0.0 9.904363466368743E-5 0.0 2.490600759070416 0.0 58-59 0.0 9.904363466368743E-5 0.0 2.82833955327359 0.0 60-61 0.0 9.904363466368743E-5 0.0 3.1957914378758705 0.0 62-63 0.0 9.904363466368743E-5 0.0 3.55596361533037 0.0 64-65 0.0 9.904363466368743E-5 0.0 3.941689050528101 0.0 66-67 0.0 9.904363466368743E-5 0.0 4.390703368275927 0.0 68-69 0.0 9.904363466368743E-5 0.0 4.904393179459142 0.0 70-71 0.0 9.904363466368743E-5 0.0 5.454927222737249 0.0 72-73 0.0 9.904363466368743E-5 0.0 5.9687655993724595 0.0 74-75 0.0 9.904363466368743E-5 0.0 6.550745996656287 0.0 76-77 0.0 9.904363466368743E-5 0.0 7.140947015617201 0.0 78-79 0.0 9.904363466368743E-5 0.0 7.880505835650954 0.0 80-81 0.0 9.904363466368743E-5 0.0 8.654185187826348 0.0 82-83 0.0 9.904363466368743E-5 0.0 9.389683218838892 0.0 84-85 0.0 9.904363466368743E-5 0.0 10.174108805375296 0.0 86-87 0.0 9.904363466368743E-5 0.0 10.962000919124929 0.0 88-89 0.0 9.904363466368743E-5 0.0 11.83170307510677 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 70 0.008528035 27.05292 6 GTACATG 4980 0.0 25.74552 1 TACATGG 5075 0.0 24.176634 2 GAGTACT 4430 0.0 23.778763 12-13 AGTACTT 4440 0.0 23.032112 12-13 ACATGGG 5110 0.0 22.805853 3 CATGGGG 2275 0.0 22.697458 4 GTACTTT 4710 0.0 22.215487 14-15 CCTATAG 245 7.54153E-9 21.320326 1 GGACCGT 160 5.1176656E-5 20.71239 6 GTGTAAG 305 2.0918378E-10 20.24001 1 GTAAGGT 235 1.16389856E-7 20.15878 4 TAAGGTG 215 1.1065422E-6 19.830614 5 TACTTTT 5150 0.0 19.811829 14-15 CATGGGA 2780 0.0 19.767206 4 ACTTTTT 5390 0.0 19.721703 16-17 AGAGTAC 7660 0.0 19.684883 10-11 ATGGGAG 1000 0.0 18.949253 5 GTAGTAC 155 9.797806E-4 18.337986 3 TGGACCG 165 0.0014900955 17.226595 5 >>END_MODULE