##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939202_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1009656 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50497496176916 34.0 31.0 34.0 31.0 34.0 2 32.706946722448045 34.0 31.0 34.0 31.0 34.0 3 32.81132583771106 34.0 31.0 34.0 31.0 34.0 4 36.24427923966183 37.0 35.0 37.0 35.0 37.0 5 36.2132766011394 37.0 37.0 37.0 35.0 37.0 6 36.27644266958251 37.0 37.0 37.0 35.0 37.0 7 36.23146497420904 37.0 37.0 37.0 35.0 37.0 8 36.21698281394851 37.0 37.0 37.0 35.0 37.0 9 37.95378425919323 39.0 38.0 39.0 35.0 39.0 10-11 37.86986755885172 39.0 38.0 39.0 35.0 39.0 12-13 37.803126510415424 39.0 38.0 39.0 35.0 39.0 14-15 39.161282654686346 40.5 38.5 41.0 36.0 41.0 16-17 39.285810711767176 41.0 39.0 41.0 36.0 41.0 18-19 39.3313405754039 41.0 39.0 41.0 36.0 41.0 20-21 39.297585019056 41.0 39.0 41.0 36.0 41.0 22-23 39.224822117632144 41.0 39.0 41.0 36.0 41.0 24-25 39.122579373568826 41.0 39.0 41.0 35.0 41.0 26-27 38.95624103655106 40.0 38.5 41.0 35.0 41.0 28-29 38.81768889602003 40.0 38.0 41.0 35.0 41.0 30-31 38.63028199703662 40.0 38.0 41.0 35.0 41.0 32-33 38.498821875965675 40.0 38.0 41.0 35.0 41.0 34-35 38.33907538805296 40.0 38.0 41.0 34.0 41.0 36-37 38.16726687109273 40.0 37.5 41.0 33.5 41.0 38-39 38.014811480345784 40.0 37.5 41.0 33.5 41.0 40-41 37.87094416316052 40.0 37.0 41.0 33.0 41.0 42-43 37.64354889189981 40.0 37.0 41.0 33.0 41.0 44-45 37.347702583850335 40.0 37.0 41.0 33.0 41.0 46-47 36.98910965715055 40.0 36.0 41.0 31.5 41.0 48-49 36.586509662697 40.0 36.0 41.0 31.0 41.0 50-51 36.29701502293851 39.0 35.0 41.0 30.5 41.0 52-53 36.05592300744016 39.0 35.0 41.0 30.0 41.0 54-55 35.821816539494634 39.0 35.0 40.0 29.0 41.0 56-57 35.55884231857187 38.5 35.0 40.0 28.5 41.0 58-59 35.21036570871664 38.0 35.0 40.0 28.0 41.0 60-61 34.7626562908555 37.5 34.0 40.0 27.5 41.0 62-63 34.3928828234567 37.0 34.0 40.0 26.0 41.0 64-65 33.945544324007386 36.5 34.0 39.0 26.0 41.0 66-67 33.47716400437377 36.0 33.0 39.0 25.5 40.0 68-69 32.96892852615148 35.0 33.0 38.5 24.0 40.0 70-71 32.414061819075016 35.0 32.5 37.5 22.5 39.5 72-73 31.87026175251769 35.0 32.0 37.0 21.0 39.0 74-75 31.391074781905914 35.0 31.0 36.0 19.5 39.0 76-77 29.421766423415498 32.5 28.5 34.5 16.0 36.0 78-79 30.407807213546 34.0 30.5 35.0 17.0 37.0 80-81 30.420424382165805 34.0 31.0 35.0 15.5 37.0 82-83 30.087570420024246 34.0 30.5 35.0 9.0 36.0 84-85 29.682125397164974 34.0 30.0 35.0 4.5 36.0 86-87 29.107626756043643 34.0 29.5 35.0 2.0 35.0 88-89 28.479532137678575 33.5 29.0 35.0 2.0 35.0 90-91 27.970464692925113 33.0 27.5 35.0 2.0 35.0 92-93 27.61122402085463 33.0 27.0 35.0 2.0 35.0 94-95 27.148836336336338 33.0 26.5 34.5 2.0 35.0 96-97 26.44291570594341 32.5 25.0 34.0 2.0 35.0 98-99 25.414539704612267 32.0 22.0 34.0 2.0 35.0 100-101 19.802629311369415 25.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15557831716602522 1101 2 0.10421401104333228 1101 3 0.09849448202983524 1101 4 0.14627460062580866 1101 5 0.1805762945155891 1101 6 0.15093655617526025 1101 7 0.16137542886139045 1101 8 0.17711901984151268 1101 9 0.14302784501327181 1101 10-11 0.08859435456279385 1101 12-13 0.19974322568481995 1101 14-15 0.19306316170866467 1101 16-17 0.15544650686810968 1101 18-19 0.20312896352489673 1101 20-21 0.13238794608424342 1101 22-23 0.10833592003166359 1101 24-25 0.1318761689259489 1101 26-27 0.1075267970532181 1101 28-29 0.11371655563807082 1101 30-31 0.22888374157550118 1101 32-33 0.1817752385542022 1101 34-35 0.22251006898096648 1101 36-37 0.3126174610409862 1101 38-39 0.22949176099051272 1101 40-41 0.25909599296508645 1101 42-43 0.4147507066765712 1101 44-45 0.2781954154351425 1101 46-47 0.48695743788465506 1101 48-49 0.6193330934022825 1101 50-51 0.4003617069414389 1101 52-53 0.4555933076252927 1101 54-55 0.502084522917265 1101 56-57 0.27973015322260153 1101 58-59 0.2858435629070115 1101 60-61 0.3307766645047394 1101 62-63 0.45424195379084864 1101 64-65 0.33450065541617136 1101 66-67 0.3332115920987704 1101 68-69 0.3459741404120322 1101 70-71 0.3651158499528506 1101 72-73 0.45242858188857227 1101 74-75 0.3375521612107839 1101 76-77 0.27435027197397943 1101 78-79 0.2930215626809449 1101 80-81 0.29580573133872434 1101 82-83 0.17733019570717445 1101 84-85 0.28060917467792734 1101 86-87 0.3811467515929756 1101 88-89 0.24809249969609226 1101 90-91 0.36107699386195335 1101 92-93 0.15024426272748315 1101 94-95 0.07768231029167438 1101 96-97 0.2854726710838662 1101 98-99 0.46953883185686607 1101 100-101 -0.18004277675661484 1102 1 0.058281477638139734 1102 2 0.15004471956436305 1102 3 0.14371752174076846 1102 4 0.15082394511608044 1102 5 0.19127661424744247 1102 6 0.19901222208518732 1102 7 0.19004580790409875 1102 8 0.16718831488626762 1102 9 0.2568862596662598 1102 10-11 0.24605560639025015 1102 12-13 0.2483686164351795 1102 14-15 0.31667696366508835 1102 16-17 0.3112927562589576 1102 18-19 0.19078318362878832 1102 20-21 0.28667490839391974 1102 22-23 0.19834890542384187 1102 24-25 0.32221690918245116 1102 26-27 0.4278744905860776 1102 28-29 0.433032400788008 1102 30-31 0.26751082607266596 1102 32-33 0.3602233504722463 1102 34-35 0.3755452929715588 1102 36-37 0.5357270018870253 1102 38-39 0.433959686774692 1102 40-41 0.5805379360789686 1102 42-43 0.6884029893718235 1102 44-45 0.7699257709436793 1102 46-47 0.6996645504326438 1102 48-49 0.6959253639738776 1102 50-51 0.5438465761366444 1102 52-53 0.7580697437442865 1102 54-55 0.7495208736952037 1102 56-57 0.7200909510070659 1102 58-59 0.6737710111140203 1102 60-61 0.4553398561180231 1102 62-63 0.42683594188113716 1102 64-65 0.39120328696383666 1102 66-67 0.6220273659330573 1102 68-69 0.9028743757536901 1102 70-71 0.6683997872901628 1102 72-73 0.7008982667922403 1102 74-75 0.4837284535098796 1102 76-77 0.6870086521577328 1102 78-79 0.998819971153246 1102 80-81 0.9480029963833552 1102 82-83 0.7726405970954566 1102 84-85 0.998167980378426 1102 86-87 0.9467266964422016 1102 88-89 0.945648007047545 1102 90-91 1.1549168070149385 1102 92-93 1.0137995858468294 1102 94-95 0.7286107509748803 1102 96-97 0.7037493877111096 1102 98-99 0.7900239565998355 1102 100-101 0.06512484474743374 1103 1 -0.03800738755828803 1103 2 0.05684100451190943 1103 3 0.13618863606062348 1103 4 0.024572045754936767 1103 5 0.1384163092862991 1103 6 0.11491564639197094 1103 7 0.12963283152473082 1103 8 0.02963119413586668 1103 9 0.11702332148399108 1103 10-11 0.1612405614911765 1103 12-13 0.17644474199028792 1103 14-15 -0.06982658880484394 1103 16-17 0.15327850160605294 1103 18-19 0.2525834282021364 1103 20-21 0.19246029947343146 1103 22-23 0.13917698490737962 1103 24-25 0.09062637086922365 1103 26-27 0.08211800617413445 1103 28-29 0.16322727594086217 1103 30-31 0.2893181504009945 1103 32-33 0.19960105959784613 1103 34-35 0.3996157603193353 1103 36-37 0.23592806133177646 1103 38-39 0.3380506938068706 1103 40-41 0.33429678227478377 1103 42-43 0.42338089661863876 1103 44-45 0.5039795323083567 1103 46-47 0.5992768429020856 1103 48-49 0.5763292737895611 1103 50-51 0.8374780147260594 1103 52-53 0.7247375297398122 1103 54-55 0.514589138763462 1103 56-57 0.6831579960068836 1103 58-59 0.6340803096586143 1103 60-61 0.6507326985696835 1103 62-63 0.7122158281013569 1103 64-65 0.6025269772791262 1103 66-67 0.6842221739261802 1103 68-69 0.6948037349099465 1103 70-71 0.6941030755984983 1103 72-73 0.8078487392889251 1103 74-75 0.6726683651388647 1103 76-77 0.6368081523774016 1103 78-79 0.7858655586190046 1103 80-81 0.6408647564720056 1103 82-83 0.7172918841837159 1103 84-85 0.6651697461196875 1103 86-87 0.3255012831999835 1103 88-89 0.7062830302876115 1103 90-91 0.8343137406135526 1103 92-93 0.7932316383881286 1103 94-95 0.9278533850914599 1103 96-97 0.6133852700360691 1103 98-99 0.2719027220920438 1103 100-101 -0.5431555303396642 1104 1 0.09258787212377939 1104 2 0.11909479731812667 1104 3 0.19802064095914318 1104 4 0.06142132950387946 1104 5 0.1277310156246827 1104 6 0.11868020201673346 1104 7 0.1828280391731525 1104 8 0.15743424414861096 1104 9 0.2557981130209015 1104 10-11 0.27472975925319787 1104 12-13 0.1906515047878088 1104 14-15 0.1743327655574376 1104 16-17 0.23108457377514213 1104 18-19 0.350189223384028 1104 20-21 0.3169724840194377 1104 22-23 0.2402405211456653 1104 24-25 0.2136688676433991 1104 26-27 0.2176788001340242 1104 28-29 0.2621142026181005 1104 30-31 0.17190212035813346 1104 32-33 0.24218718448386056 1104 34-35 0.274405851851256 1104 36-37 0.29000954917877664 1104 38-39 0.08272381474831292 1104 40-41 0.18198798622304224 1104 42-43 0.38021793406447557 1104 44-45 0.3881750999875848 1104 46-47 0.3537496342970883 1104 48-49 0.13943715425933334 1104 50-51 0.0643440648728344 1104 52-53 0.2915376968781942 1104 54-55 0.4099331784141569 1104 56-57 0.49712916200736146 1104 58-59 0.3519469037935252 1104 60-61 0.3325863578754351 1104 62-63 0.423553907534135 1104 64-65 0.5410589451233818 1104 66-67 0.4269703126720614 1104 68-69 0.40074003836597427 1104 70-71 0.48235174913465073 1104 72-73 0.36654558547168037 1104 74-75 0.07042355996283334 1104 76-77 0.1729662821093747 1104 78-79 0.2047002939700846 1104 80-81 0.21137035008921146 1104 82-83 0.28937491201645926 1104 84-85 0.10874411613148638 1104 86-87 0.052606477892549464 1104 88-89 0.3291763902159204 1104 90-91 0.7765320822118262 1104 92-93 0.3628253291768573 1104 94-95 0.6321872886925597 1104 96-97 0.041370569216407915 1104 98-99 0.05349213981449097 1104 100-101 -0.8036844602598023 1105 1 0.01536063855723313 1105 2 0.07996952597191154 1105 3 0.06521493049292104 1105 4 0.08713573345767145 1105 5 -0.02155423555633007 1105 6 0.03317369535080417 1105 7 0.08815188014389008 1105 8 0.15777409313527357 1105 9 0.12691257942664436 1105 10-11 0.16859592396333056 1105 12-13 0.1454584868661044 1105 14-15 0.0793945089701964 1105 16-17 0.262770789073862 1105 18-19 0.11784099229173961 1105 20-21 0.1659992744028358 1105 22-23 0.2158667023090004 1105 24-25 0.20386278221464238 1105 26-27 0.25510482654693334 1105 28-29 0.2690046094712599 1105 30-31 0.253068341626161 1105 32-33 0.20904563800794307 1105 34-35 3.9790364875358364E-4 1105 36-37 0.07472524857168139 1105 38-39 0.03468785345849312 1105 40-41 0.14293419602899604 1105 42-43 0.018095044169271546 1105 44-45 0.12047066075722057 1105 46-47 0.19353529116497725 1105 48-49 0.3324776489590633 1105 50-51 0.3242808855667505 1105 52-53 0.2901482036043177 1105 54-55 0.25071267410836384 1105 56-57 0.3041425916855829 1105 58-59 0.4807741238162748 1105 60-61 0.42929784865875575 1105 62-63 0.2974918794508241 1105 64-65 0.3281259982062892 1105 66-67 0.28304928304250154 1105 68-69 0.029765399126340242 1105 70-71 -0.15965245696683183 1105 72-73 -0.1971710833059781 1105 74-75 -0.09254343455569014 1105 76-77 -0.26392589296176183 1105 78-79 -0.09155522230567215 1105 80-81 -0.025773388475371917 1105 82-83 0.05663228053189684 1105 84-85 0.029980037793162495 1105 86-87 -0.1475670833611531 1105 88-89 -0.08938024229446029 1105 90-91 0.18768163708489638 1105 92-93 0.22829027645010314 1105 94-95 -0.08486146772349912 1105 96-97 -0.4746995224424957 1105 98-99 -0.29276444240827004 1105 100-101 -0.7792667494875936 1106 1 0.017813382002934475 1106 2 -0.09075694461275674 1106 3 0.04100773553621195 1106 4 -0.012413869093570895 1106 5 0.14570418680318653 1106 6 0.07159637024827248 1106 7 0.03802469411506593 1106 8 0.06829990456030544 1106 9 0.14328967599462317 1106 10-11 0.088485708201965 1106 12-13 0.0959717798379387 1106 14-15 -0.08725254707049857 1106 16-17 6.753795372773652E-4 1106 18-19 0.060709780221621656 1106 20-21 0.06308776361233726 1106 22-23 0.17614002187042388 1106 24-25 0.0688606048286502 1106 26-27 0.17254793248192613 1106 28-29 0.1276934713230915 1106 30-31 -0.03865286077611785 1106 32-33 0.03200656757587694 1106 34-35 -0.04302366132772306 1106 36-37 0.018361276971106122 1106 38-39 -0.03473618852199678 1106 40-41 -0.03205061079015792 1106 42-43 -0.056664503274092226 1106 44-45 0.009586520739780724 1106 46-47 -0.25593601040237246 1106 48-49 -0.04883055366034483 1106 50-51 0.014407319517957262 1106 52-53 -0.11481223347407621 1106 54-55 -0.1457899223789525 1106 56-57 -0.16616125980092278 1106 58-59 -0.21044221616163128 1106 60-61 -0.16266001558638976 1106 62-63 -0.23247052879874275 1106 64-65 -0.14085686534135533 1106 66-67 -0.058539517308794586 1106 68-69 -0.2860271390507023 1106 70-71 -0.21901624782617546 1106 72-73 -0.1404917243187107 1106 74-75 -0.17687013707139343 1106 76-77 -0.08130325275689998 1106 78-79 0.14108603788528384 1106 80-81 0.10915600720600338 1106 82-83 -0.03078764443016624 1106 84-85 0.04465222293040938 1106 86-87 0.08925851833126686 1106 88-89 0.056050946849421024 1106 90-91 0.27386973169626216 1106 92-93 0.32043979918407217 1106 94-95 0.35130715778187493 1106 96-97 -0.1160640338407184 1106 98-99 0.053533940746163466 1106 100-101 -0.3130859815678271 1107 1 0.07954857246342328 1107 2 0.08583484172492462 1107 3 0.007265048232923732 1107 4 0.010777621001608395 1107 5 0.028954994345532725 1107 6 0.12166916684412143 1107 7 0.04855865456014641 1107 8 0.07181275686537703 1107 9 0.144292855602842 1107 10-11 0.09465532024839973 1107 12-13 0.10828687246256408 1107 14-15 0.018935106121233503 1107 16-17 0.18824353657108617 1107 18-19 0.28243143407754445 1107 20-21 0.16901456712358254 1107 22-23 0.07580884619677875 1107 24-25 0.24127937083729023 1107 26-27 0.043113631553318044 1107 28-29 0.29452178052304845 1107 30-31 0.2621763424372716 1107 32-33 0.3280456743438336 1107 34-35 0.30236027607755034 1107 36-37 0.40043136624604614 1107 38-39 0.30643048752913415 1107 40-41 0.3282138241370376 1107 42-43 0.33868249000242656 1107 44-45 0.4129985954370099 1107 46-47 0.26365242166122016 1107 48-49 0.26656141607649175 1107 50-51 0.3220151670714344 1107 52-53 0.4468373477300389 1107 54-55 0.32268059069053123 1107 56-57 0.5648596277085147 1107 58-59 0.5034723331602251 1107 60-61 0.3166489740750009 1107 62-63 0.3277122038717053 1107 64-65 0.2841695356340139 1107 66-67 0.2827070224110315 1107 68-69 0.4940501505947097 1107 70-71 0.4462584544661965 1107 72-73 0.45238684958045994 1107 74-75 0.2973769898474927 1107 76-77 0.34239936481793265 1107 78-79 0.4554300124300319 1107 80-81 0.32948435605051074 1107 82-83 0.3283098616400508 1107 84-85 0.3402902876785845 1107 86-87 0.46011666781536675 1107 88-89 0.36162198357176933 1107 90-91 0.7463822656957753 1107 92-93 0.7011889778523148 1107 94-95 0.9684303879972305 1107 96-97 1.1458037647833308 1107 98-99 1.0634456540459354 1107 100-101 -0.11234319199308018 1108 1 -0.09702748138850836 1108 2 -0.03355910171097776 1108 3 -0.019272990919994015 1108 4 0.00790095313447381 1108 5 0.04082402814971431 1108 6 0.04087681602403137 1108 7 0.09738975814241968 1108 8 0.10378067334863772 1108 9 0.027338744457644282 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-1.801830320076661 2316 90-91 -2.0243427935042355 2316 92-93 -2.1622178321904926 2316 94-95 -2.2799503118266244 2316 96-97 -2.1286910423354435 2316 98-99 -1.8211219039584101 2316 100-101 -0.5678103957694383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 13.0 6 71.0 7 126.0 8 261.0 9 533.0 10 958.0 11 1403.0 12 2191.0 13 3185.0 14 3714.0 15 5002.0 16 6703.0 17 9767.0 18 9600.0 19 6244.0 20 5798.0 21 6336.0 22 6600.0 23 6955.0 24 7842.0 25 8979.0 26 10042.0 27 11860.0 28 14400.0 29 17997.0 30 21731.0 31 26779.0 32 33617.0 33 44984.0 34 64756.0 35 102984.0 36 169529.0 37 250478.0 38 142965.0 39 5247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.823384539482845 18.75708709819883 14.553468052853342 21.86606030946498 2 15.799143470647428 22.13585617279549 37.0600481748239 25.004952181733188 3 21.61835318167772 24.166151639766415 30.15759823147686 24.057896947079005 4 13.369008850539194 16.803346882502556 36.62901027676753 33.198633990190714 5 13.562242981768048 38.10931644045101 33.09295443200456 15.235486145776383 6 31.214690944242395 35.55616962609047 16.24563217571133 16.983507253955803 7 27.587217824684846 33.663841942206055 20.41319023508997 18.33574999801913 8 25.934179562147904 31.66207104201827 19.648078157312987 22.75567123852084 9 25.308719009246715 18.311484307526523 21.73274857971428 34.64704810351248 10-11 25.94576766740355 25.347890766756205 26.105822181020073 22.600519384820174 12-13 28.066143320101105 23.46363513909688 26.41741345567203 22.052808085129985 14-15 23.597033019493963 25.375230526342897 27.1174748377154 23.910261616447745 16-17 21.726047803366757 28.566532889080815 27.21703663498528 22.490382672567144 18-19 21.393969827347135 28.075601987211485 29.022360090961673 21.508068094479704 20-21 23.16071018247799 25.640812316274058 28.156223505827725 23.04225399542022 22-23 22.12733842021441 25.735844683733866 29.07212951738018 23.064687378671547 24-25 23.835084179786254 25.926393632673356 28.433885056719898 21.804637130820492 26-27 22.758040409302914 25.238926763462256 30.522603080964206 21.480429746270623 28-29 21.245206288082276 28.11115865205575 29.058560539431248 21.585074520430723 30-31 21.901865585902524 25.98360233584508 28.776830920630392 23.337701157622 32-33 22.15586298699755 26.532205028247247 29.08376714445316 22.228164840302043 34-35 23.6857563135503 25.83392353905511 28.39010039275508 22.090219754639513 36-37 22.873315315815127 25.33723404676893 29.872308765311256 21.917141872104683 38-39 22.07923626644688 26.651275629989048 29.392042493922503 21.877445609641573 40-41 21.926073623277983 26.899814971096664 28.899672336396016 22.274439069229338 42-43 21.976429522330342 26.995952724333577 27.27765737424324 23.74996037909284 44-45 22.46006783750496 25.724716811350028 27.93624522530045 23.878970125844564 46-47 23.62865583236967 26.974081383514797 27.84794015466483 21.5493226294507 48-49 23.159931974608345 26.3639001884862 27.951918686480003 22.524249150425458 50-51 21.006836544437537 27.042309320827318 29.337021089043454 22.61383304569169 52-53 22.617455846347667 27.91703312811492 26.6145598104701 22.85095121506731 54-55 21.478602613167258 26.326788529954758 27.6118796897161 24.582729167161883 56-57 22.8768624545699 26.172194377191033 27.28935760761963 23.66158556061944 58-59 24.262597899751643 25.713457981414212 27.109694768197656 22.91424935063649 60-61 22.557786192326475 26.552726898151498 28.932070952936083 21.957415956585947 62-63 21.434082575804098 26.92627683872105 28.5096103371294 23.130030248345452 64-65 21.16255275484017 28.146397219556214 27.412856085885522 23.278193939718093 66-67 22.105089045040778 26.618824033964934 26.577323661798708 24.698763259195577 68-69 21.461773815407746 26.66220289240686 27.497328173580073 24.37869511860532 70-71 22.977425930071092 26.545133283283185 26.766842898656375 23.71059788798935 72-73 22.38089012495345 25.53171575269201 28.583002527593553 23.50439159476099 74-75 21.10080066874262 28.288397236286418 26.857910020838784 23.752892074132177 76-77 21.636743960107463 26.309841282430035 26.95442694134092 25.098987816121582 78-79 21.84313678873809 26.602168815769534 27.43075470707515 24.12393968841723 80-81 22.651292377266998 26.399567771437855 26.93158328967138 24.017556561623767 82-83 21.800841078545563 25.797796477216 28.13884134794425 24.262521096294183 84-85 21.32637254668917 26.492439008929775 27.53477422013042 24.646414224250634 86-87 21.52494980293002 26.57876601968221 27.19754096328797 24.6987432140998 88-89 21.030731553010785 26.52493917153924 26.221990557910885 26.22233871753909 90-91 21.620442697647444 25.211523090597186 27.046406009936646 26.12162820181872 92-93 22.29521738096936 25.99192200115683 27.107450458373943 24.60541015949987 94-95 21.4528512681547 25.38354647523513 27.482281093758665 25.681321162851507 96-97 20.22109510565975 25.511510851220613 28.581417829438937 25.6859762136807 98-99 21.030205937191262 26.287262201599066 26.53749681202196 26.145035049187715 100-101 20.905559090875325 24.28246352086485 27.495301601293516 27.31667578696631 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1537.0 1 1034.5 2 675.5 3 809.0 4 695.0 5 717.0 6 1196.5 7 2231.0 8 3220.5 9 3351.5 10 2692.5 11 2112.0 12 1999.5 13 2085.0 14 2140.0 15 2051.0 16 2029.5 17 2026.0 18 2070.5 19 2155.0 20 2275.5 21 2495.5 22 2690.5 23 2954.5 24 3329.0 25 3762.5 26 4320.5 27 4914.5 28 5587.0 29 6520.0 30 7614.5 31 9008.0 32 11133.0 33 12638.5 34 13122.5 35 14025.5 36 15607.5 37 17062.0 38 18004.5 39 18628.0 40 19676.5 41 22567.0 42 25206.5 43 27216.5 44 29153.5 45 31037.0 46 34258.5 47 47873.0 48 72873.0 49 71300.0 50 50345.0 51 40821.5 52 36470.5 53 34916.5 54 33718.5 55 31367.0 56 28216.5 57 25057.0 58 22975.0 59 20780.5 60 17914.5 61 15475.5 62 13149.0 63 10462.0 64 7958.0 65 6209.0 66 4630.5 67 3902.0 68 3045.0 69 2097.5 70 1636.0 71 1317.5 72 1124.5 73 934.5 74 716.5 75 584.0 76 381.0 77 199.0 78 133.5 79 87.0 80 46.5 81 26.5 82 12.0 83 5.5 84 7.5 85 4.5 86 5.5 87 5.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4266799781311655 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.904363466368743E-4 16-17 0.0019808726932737486 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4856545199553117E-4 26-27 0.003664614482556435 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01198427979430618 36-37 0.026543694089868234 38-39 0.019214465124755362 40-41 0.008319665311749744 42-43 0.008715839850404495 44-45 0.0037141362998882785 46-47 0.005298834454507278 48-49 0.003268439943901685 50-51 8.418708946413432E-4 52-53 0.0 54-55 0.0 56-57 4.9521817331843714E-5 58-59 0.0013370890679597805 60-61 0.0028722654052469355 62-63 0.001386610885291624 64-65 0.003268439943901685 66-67 0.0026246563185877174 68-69 0.0046550508291933095 70-71 1.9808726932737486E-4 72-73 0.0 74-75 0.0 76-77 0.09369527839184831 78-79 0.016342199719508427 80-81 4.952181733184371E-4 82-83 0.0 84-85 0.0 86-87 0.11201835080463049 88-89 0.4328206834803141 90-91 0.4345539470869286 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.46652721322906E-4 100-101 3.46652721322906E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1009656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.83303433068668 #Duplication Level Percentage of deduplicated Percentage of total 1 65.16438817771845 23.350377587103786 2 15.147872015056965 10.855884359047685 3 6.796512299267844 7.306189756457969 4 3.8142374392347578 5.467028044219581 5 2.2493603888124984 4.030070401720249 6 1.499266642976199 3.2233963853171703 7 1.0255318908535265 2.5723543616517905 8 0.7499868075090935 2.149944241682836 9 0.5766041771494164 1.8595329547511146 >10 2.7037839031400117 17.177786335808428 >50 0.17076970432946856 4.242431644928034 >100 0.08869941255690302 5.381246216567136 >500 0.004973798832092708 1.2643175998120557 >1k 0.0063554096187851275 6.028108684639186 >5k 0.001381610786692419 3.3743190135480496 >10k+ 2.763221573384838E-4 1.7170124127449962 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17341 1.7175156687030038 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9713 0.962010823488396 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7224 0.715491216810478 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6508 0.6445759743912778 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5560 0.5506826087301022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5074 0.50254740228355 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4650 0.46055290118614656 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4507 0.44638966142923925 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4274 0.42331249455260006 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4251 0.4210344909553353 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4080 0.40409802942784473 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3872 0.38349695341779777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3699 0.3663624046209798 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3499 0.34655367768824236 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3019 0.29901273304967235 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2431 0.24077507586742417 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2422 0.23988368315545097 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2347 0.23245541055567442 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2295 0.22730514155316267 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2263 0.22413574524392466 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2126 0.21056676729499946 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1698 0.16817609165894126 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1659 0.16431338990705743 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1514 0.14995206288082277 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1446 0.14321709572369204 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1317 0.13044046685207636 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1277 0.12647872146552885 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.11627722709516904 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1061 0.10508529637817236 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.9521817331843714E-5 0.0 20-21 0.0 0.0 0.0 1.9808726932737486E-4 0.0 22-23 0.0 0.0 0.0 0.0012875672506279367 0.0 24-25 0.0 0.0 0.0 0.0032684399439016857 0.0 26-27 0.0 0.0 0.0 0.00693305442645812 0.0 28-29 0.0 0.0 0.0 0.020650597827378832 0.0 30-31 0.0 0.0 0.0 0.05224551728509512 0.0 32-33 0.0 0.0 0.0 0.1071652127061098 0.0 34-35 0.0 0.0 0.0 0.18164602597320276 0.0 36-37 0.0 0.0 0.0 0.28722654052469354 0.0 38-39 0.0 0.0 0.0 0.4335139889229599 0.0 40-41 0.0 0.0 0.0 0.5995606424366319 0.0 42-43 0.0 0.0 0.0 0.7599618087744737 0.0 44-45 0.0 0.0 0.0 0.9580490781018486 0.0 46-47 0.0 0.0 0.0 1.201300244835865 0.0 48-49 0.0 0.0 0.0 1.4234551173865158 0.0 50-51 0.0 0.0 0.0 1.6717575094883803 0.0 52-53 0.0 0.0 0.0 1.904113876409391 0.0 54-55 0.0 0.0 0.0 2.174899173579912 0.0 56-57 0.0 0.0 0.0 2.522740418518783 0.0 58-59 0.0 0.0 0.0 2.865530438089805 0.0 60-61 0.0 0.0 0.0 3.2370431117132963 0.0 62-63 0.0 0.0 0.0 3.6022665145356436 0.0 64-65 0.0 0.0 0.0 3.992944131466559 0.0 66-67 0.0 0.0 0.0 4.445524020062279 0.0 68-69 0.0 0.0 0.0 4.963868882074687 0.0 70-71 0.0 0.0 0.0 5.521682632500575 0.0 72-73 0.0 0.0 0.0 6.040621756320965 0.0 74-75 0.0 0.0 0.0 6.627752422607304 0.0 76-77 0.0 0.0 0.0 7.223945581465371 0.0 78-79 0.0 4.9521817331843714E-5 0.0 7.967812799606995 0.0 80-81 0.0 9.904363466368743E-5 0.0 8.743572068110328 0.0 82-83 0.0 9.904363466368743E-5 0.0 9.479317708209528 0.0 84-85 0.0 9.904363466368743E-5 0.0 10.267011734689834 0.0 86-87 0.0 9.904363466368743E-5 0.0 11.057429461123393 0.0 88-89 0.0 9.904363466368743E-5 0.0 11.928221097086531 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGA 60 0.0039707073 31.634048 2 GTAAGCG 255 0.0 29.773958 94-95 TCGACGT 35 0.008435346 27.114899 16-17 CCTAACC 95 0.0012338413 24.974249 3 GTACATG 4660 0.0 24.335901 1 ATGGGAG 850 0.0 24.00466 5 TACATGG 4780 0.0 23.526995 2 ACATGGG 4720 0.0 22.720215 3 GAGTACT 4730 0.0 22.272009 12-13 CCGTGCA 200 5.4358134E-7 21.354038 9 CATGGGA 2645 0.0 21.1691 4 ACCGTGC 135 3.8220084E-4 21.089365 8 GTACTTT 4955 0.0 21.06913 14-15 AGTACTT 4830 0.0 21.024912 12-13 GTGTAAG 250 9.149517E-9 20.965687 1 TCTAGGA 275 1.2751116E-9 20.705923 2 GAATCCG 115 0.0037441899 20.631922 9 TCTATGT 665 0.0 19.9794 2 TATAGCA 190 8.323412E-6 19.979399 2 CTATGTC 670 0.0 19.8303 3 >>END_MODULE