FastQCFastQC Report
Fri 27 May 2016
SRR939201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908981
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT170510.8931990417924536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124380.6515517964819975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96970.5079673396435062No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT92030.4820896593522932No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT65990.3456818061573164No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC65220.341648240605852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64160.33609553997656344No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44990.23567547293556093No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37800.19801139979915985No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36090.18905374123681692No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35130.18402488028953667No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35130.18402488028953667No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30790.16129023809037388No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30750.1610807022175705No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30410.1592996472987421No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26460.13860797985941192No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25030.13111707240669235No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23010.12053551083012351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22720.11901637575229926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.11089686068116969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG127000.024.5602841
TACATGG128000.023.8022232
GAGTACT90050.023.16751112-13
CATGGGG38050.022.9128784
ACATGGG127950.022.8857253
GTAAGCG2350.021.24886594-95
GTACTTT98150.021.23283614-15
AGTACTT93600.020.39136912-13
GTATTAC4600.019.6095451
TACCTGG13450.019.3756772
CATGGGA87100.019.0943764
TAGGACG1250.00611959918.9529364
ACTTTTT114000.018.46986816-17
ATGGGAG32750.018.2295425
AGTACAT97450.017.7471032
TACTTTT109650.017.40772614-15
ATGGGGG20200.016.8887565
GTGTAGC10200.016.7561441
TACCCGG2002.8142644E-416.5838182
GTGTAAG8700.016.3709451
Produced by FastQC (version 0.11.5)