##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939201_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1908981 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22556012867598 33.0 31.0 34.0 31.0 34.0 2 32.29990921858311 34.0 31.0 34.0 31.0 34.0 3 32.35935821257519 34.0 31.0 34.0 30.0 34.0 4 35.78614087830104 37.0 35.0 37.0 35.0 37.0 5 35.84756631941334 37.0 35.0 37.0 35.0 37.0 6 35.95134577033507 37.0 37.0 37.0 35.0 37.0 7 35.89127864551821 37.0 37.0 37.0 35.0 37.0 8 35.89082290499486 37.0 37.0 37.0 35.0 37.0 9 37.577550536123724 39.0 38.0 39.0 35.0 39.0 10-11 37.499167356825446 39.0 38.0 39.0 35.0 39.0 12-13 37.42059795252022 39.0 38.0 39.0 35.0 39.0 14-15 38.715200151284904 40.0 38.0 41.0 35.0 41.0 16-17 38.67986297401598 40.0 38.0 41.0 35.0 41.0 18-19 38.66431253113572 40.0 38.0 41.0 34.5 41.0 20-21 38.58313519097361 40.0 38.0 41.0 34.0 41.0 22-23 38.50561503755145 40.0 38.0 41.0 34.0 41.0 24-25 38.534175824693904 40.0 38.0 41.0 34.0 41.0 26-27 38.44254343023843 40.0 38.0 41.0 34.0 41.0 28-29 38.33224636599317 40.0 38.0 41.0 34.0 41.0 30-31 38.15757516706557 40.0 38.0 41.0 34.0 41.0 32-33 37.93011900066057 40.0 38.0 41.0 33.5 41.0 34-35 37.59294199366049 40.0 37.0 41.0 33.0 41.0 36-37 37.33754474245684 40.0 37.0 41.0 32.5 41.0 38-39 37.107065497246964 40.0 36.5 41.0 31.5 41.0 40-41 36.97488450644611 40.0 36.5 41.0 31.0 41.0 42-43 36.76279308175408 40.0 36.0 41.0 31.0 41.0 44-45 36.457814404648346 40.0 36.0 41.0 30.0 41.0 46-47 36.03415146614869 39.5 35.0 41.0 29.5 41.0 48-49 35.92448615255993 40.0 36.0 41.0 28.0 41.0 50-51 34.893584587798415 38.5 34.5 40.0 26.0 40.5 52-53 35.06890901480947 38.5 35.0 40.0 26.5 40.5 54-55 35.59087151731735 39.0 35.0 41.0 27.0 41.0 56-57 35.475001584615036 39.0 35.0 41.0 27.0 41.0 58-59 35.311192987253406 39.0 35.0 41.0 26.0 41.0 60-61 35.00921643536525 39.0 35.0 41.0 26.0 41.0 62-63 34.68468046565157 38.0 35.0 40.5 25.5 41.0 64-65 34.33680900962346 38.0 34.5 40.0 24.5 41.0 66-67 33.93280315519117 37.0 34.0 40.0 22.5 41.0 68-69 33.518277814184636 36.5 34.0 39.0 22.0 41.0 70-71 33.09729143454021 36.0 33.5 39.0 21.5 41.0 72-73 32.646889885231964 35.5 33.0 39.0 19.5 40.0 74-75 32.205826302095204 35.0 33.0 37.5 18.0 40.0 76-77 31.776915275741352 35.0 32.0 37.0 15.5 39.0 78-79 31.346693602503116 35.0 32.0 36.5 10.0 39.0 80-81 30.895960724596 35.0 31.5 36.0 7.0 38.5 82-83 30.477442677533197 35.0 31.0 36.0 2.0 37.0 84-85 30.100843853343747 35.0 31.0 35.0 2.0 37.0 86-87 29.761226539185042 34.0 31.0 35.0 2.0 36.5 88-89 29.363398326122677 34.0 30.0 35.0 2.0 36.0 90-91 28.97371974891316 34.0 29.5 35.0 2.0 36.0 92-93 28.587062155149788 34.0 29.0 35.0 2.0 35.5 94-95 28.155326061390866 34.0 29.0 35.0 2.0 35.0 96-97 27.630570707618357 34.0 28.5 35.0 2.0 35.0 98-99 26.9977042725936 34.0 26.5 35.0 2.0 35.0 100-101 24.50579366688301 31.5 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3388107772705524 1101 2 0.3786459983513666 1101 3 0.41462387385131194 1101 4 0.42766573263758545 1101 5 0.44056486721913046 1101 6 0.3855922958132538 1101 7 0.2856697388159688 1101 8 0.3774566633355647 1101 9 0.3655856086005187 1101 10-11 0.4234058469097377 1101 12-13 0.4948302376022724 1101 14-15 0.5457250803253473 1101 16-17 0.5623729776693551 1101 18-19 0.435972148951592 1101 20-21 0.5598493318330782 1101 22-23 0.5520860945168593 1101 24-25 0.5624348956155956 1101 26-27 0.47735886977640973 1101 28-29 0.4363083815395683 1101 30-31 0.4835596431282738 1101 32-33 0.5286012163522571 1101 34-35 0.5364801018820202 1101 36-37 0.539015795525863 1101 38-39 0.42176779272872267 1101 40-41 0.20642412212358607 1101 42-43 0.129779974483462 1101 44-45 0.22889199010018757 1101 46-47 0.26524296991621554 1101 48-49 0.3497894590814141 1101 50-51 0.2321899244455352 1101 52-53 0.27439527046797707 1101 54-55 0.2751128418369504 1101 56-57 0.3501431990272508 1101 58-59 0.3661735244772757 1101 60-61 0.2387263634172072 1101 62-63 0.20896982124699548 1101 64-65 0.22463214391417807 1101 66-67 0.19723373416022838 1101 68-69 0.1358636792711323 1101 70-71 0.006649443038007519 1101 72-73 0.0941984708235779 1101 74-75 0.03916127905770139 1101 76-77 -0.14808522065663965 1101 78-79 -0.08303502493872728 1101 80-81 -0.07266522101900819 1101 82-83 -0.13647089138924784 1101 84-85 -0.23984731065191411 1101 86-87 -0.26846152321344263 1101 88-89 -0.2894225285430885 1101 90-91 -0.3468453763960291 1101 92-93 -0.25415011855415415 1101 94-95 -0.6126438262360772 1101 96-97 -0.8890505896641834 1101 98-99 -1.020015742137506 1101 100-101 -0.7702484474597746 1102 1 0.04699309508642102 1102 2 0.1214009670094569 1102 3 0.16030730571608132 1102 4 0.14906364119644877 1102 5 0.16552253336095646 1102 6 0.1811390710130354 1102 7 0.2306171557079253 1102 8 0.16821451366680407 1102 9 0.17135489650046765 1102 10-11 0.09916599960870798 1102 12-13 0.0649276797459919 1102 14-15 0.12971434089395473 1102 16-17 0.27761353796167754 1102 18-19 0.22630034775733066 1102 20-21 0.23927804755750515 1102 22-23 0.3071431338205102 1102 24-25 0.21787405369170898 1102 26-27 0.1686455219851979 1102 28-29 0.19203125073537564 1102 30-31 0.14869993901966438 1102 32-33 0.20065307161502943 1102 34-35 0.05970505063577036 1102 36-37 0.16426422843041166 1102 38-39 0.1899579831568161 1102 40-41 0.2423811643980187 1102 42-43 0.25378698556453827 1102 44-45 0.02397680635639432 1102 46-47 -0.11125022695851072 1102 48-49 0.07226799507262172 1102 50-51 -0.09082372636697755 1102 52-53 -0.10522617571603377 1102 54-55 -0.2272404007880553 1102 56-57 -0.26055575525219155 1102 58-59 -0.28117689589051054 1102 60-61 -0.3670121024631854 1102 62-63 -0.25899533130343855 1102 64-65 -0.2334937287574661 1102 66-67 -0.28441453832127905 1102 68-69 -0.4264955792179208 1102 70-71 -0.5620546952823062 1102 72-73 -0.5410622908887817 1102 74-75 -0.45572560784405525 1102 76-77 -0.5429487125315084 1102 78-79 -0.4004279277045377 1102 80-81 -0.5423827724880681 1102 82-83 -0.5145612630359615 1102 84-85 -0.5220299552554586 1102 86-87 -0.6199212986211826 1102 88-89 -0.6495202677665226 1102 90-91 -0.8208913791885806 1102 92-93 -0.7795964808131473 1102 94-95 -0.8384162334729375 1102 96-97 -0.9719202310889834 1102 98-99 -0.9813749560646023 1102 100-101 -1.048602477345998 1103 1 0.2779990804439052 1103 2 0.294573235580728 1103 3 0.3435410286142542 1103 4 0.250904215022409 1103 5 0.23116719259721918 1103 6 0.3012987969642822 1103 7 0.15798885179204802 1103 8 0.2616947960394711 1103 9 0.3316811297707858 1103 10-11 0.36347090213018163 1103 12-13 0.35098316472293334 1103 14-15 0.38471923774003614 1103 16-17 0.439150158075563 1103 18-19 0.41489768148648665 1103 20-21 0.4708846787456906 1103 22-23 0.3687899147808693 1103 24-25 0.4043874992814196 1103 26-27 0.44156174298794326 1103 28-29 0.527854481140352 1103 30-31 0.49594089731715485 1103 32-33 0.5696140525558917 1103 34-35 0.49335060338272996 1103 36-37 0.4274176808888015 1103 38-39 0.4849463922566528 1103 40-41 0.5383864148647888 1103 42-43 0.3980187482690525 1103 44-45 0.4101928031083162 1103 46-47 0.6601467348140773 1103 48-49 0.7334608173003758 1103 50-51 0.8659395966196755 1103 52-53 0.702559518508302 1103 54-55 0.78187006129086 1103 56-57 0.78111655042742 1103 58-59 0.7684198601126724 1103 60-61 0.7225474287783982 1103 62-63 0.7252307123505233 1103 64-65 0.8028127832474468 1103 66-67 0.6609554318610762 1103 68-69 0.5634644310851016 1103 70-71 0.6641719537469868 1103 72-73 0.6314332961288258 1103 74-75 0.6566806572084687 1103 76-77 0.5632531830499765 1103 78-79 0.45806086048249384 1103 80-81 0.5699896584867759 1103 82-83 0.5764833295508076 1103 84-85 0.6219529632648033 1103 86-87 0.5555240378787296 1103 88-89 0.5700348832353299 1103 90-91 0.415986091972421 1103 92-93 0.3496074942907903 1103 94-95 0.3908143723244031 1103 96-97 0.24402995350683554 1103 98-99 -0.11074519109865832 1103 100-101 -0.17704318476198821 1104 1 0.15963506535636185 1104 2 0.11793890829617482 1104 3 0.20420020155175678 1104 4 0.14997344131676726 1104 5 0.22446787378846267 1104 6 0.2767315003990163 1104 7 0.22362466155891525 1104 8 0.24613811454798196 1104 9 0.2875694619469087 1104 10-11 0.30640627966275247 1104 12-13 0.21818684027824986 1104 14-15 0.44171661681139085 1104 16-17 0.43368624119465693 1104 18-19 0.4376572253573272 1104 20-21 0.35075513356125043 1104 22-23 0.35269372232088614 1104 24-25 0.37969979012318333 1104 26-27 0.4210243587873208 1104 28-29 0.39278282782853324 1104 30-31 0.2099031284217645 1104 32-33 0.21700219394842435 1104 34-35 0.27999199904287764 1104 36-37 0.21745564995058686 1104 38-39 0.3372986600792842 1104 40-41 0.2715130138501962 1104 42-43 0.1517233902526982 1104 44-45 0.16455580485351362 1104 46-47 0.15400256497289888 1104 48-49 0.33072917161990034 1104 50-51 0.2072670750861505 1104 52-53 0.34733455025344284 1104 54-55 0.34164631279993074 1104 56-57 0.2318631085038234 1104 58-59 0.2755679894952223 1104 60-61 0.33175070400092466 1104 62-63 0.2802311189385023 1104 64-65 0.19258678288464637 1104 66-67 0.20510150328293975 1104 68-69 0.2614837221207651 1104 70-71 0.3310638076005006 1104 72-73 0.19011019630209347 1104 74-75 0.19368798204723703 1104 76-77 0.05467730435080753 1104 78-79 0.1298583546495209 1104 80-81 -0.023602202247460013 1104 82-83 -0.07135362241551135 1104 84-85 -0.06901202638283976 1104 86-87 -0.05719309573859377 1104 88-89 -0.045431410621436186 1104 90-91 -0.25879982382008393 1104 92-93 -0.2616693796106375 1104 94-95 -0.32970174419745746 1104 96-97 -0.5130134012818317 1104 98-99 -0.5345865717463241 1104 100-101 -0.39562018939709276 1105 1 0.2648222509119975 1105 2 0.2939158180512891 1105 3 0.2473727846258882 1105 4 0.25853073873164334 1105 5 0.2278794187104225 1105 6 0.24144189164407237 1105 7 0.1035817181942349 1105 8 0.19821008511446792 1105 9 0.2614472524553264 1105 10-11 0.25049236152847243 1105 12-13 0.31220530940008473 1105 14-15 0.40139623231257104 1105 16-17 0.28348871575091295 1105 18-19 0.3433843104511496 1105 20-21 0.35790133446683114 1105 22-23 0.3726402335734704 1105 24-25 0.30334266519013653 1105 26-27 0.3490880414047126 1105 28-29 0.31456572593094023 1105 30-31 0.34418484451239806 1105 32-33 0.31892970012244604 1105 34-35 0.38115900803351366 1105 36-37 0.31585399476655596 1105 38-39 0.45011287055055504 1105 40-41 0.3780748415026878 1105 42-43 0.230891614835933 1105 44-45 0.2623371645863912 1105 46-47 0.27073270107717207 1105 48-49 0.40932000642595057 1105 50-51 0.4360297067405412 1105 52-53 0.4766698603448276 1105 54-55 0.44763826877128565 1105 56-57 0.36463602625017444 1105 58-59 0.39880177989538623 1105 60-61 0.5618513896846977 1105 62-63 0.5438989340361076 1105 64-65 0.5785922313323937 1105 66-67 0.4327268976070826 1105 68-69 0.4750985597014932 1105 70-71 0.29770868849548293 1105 72-73 0.45306830313189295 1105 74-75 0.48946825850600817 1105 76-77 0.4659437226195813 1105 78-79 0.4736640616529435 1105 80-81 0.41831749847217026 1105 82-83 0.37637706534993853 1105 84-85 0.39793725386805434 1105 86-87 0.37777854767376695 1105 88-89 0.34345246620341285 1105 90-91 0.2956769684856617 1105 92-93 0.19987756388234956 1105 94-95 0.16706681104835397 1105 96-97 0.32813290454295796 1105 98-99 0.24729190975865833 1105 100-101 0.5317937626521605 1106 1 0.1286512193521716 1106 2 0.16300438478049273 1106 3 0.1226626439228724 1106 4 0.1232466073315237 1106 5 0.12138003976352962 1106 6 0.14208486240281104 1106 7 0.11633982796278985 1106 8 0.12459354901390185 1106 9 0.16700735825894952 1106 10-11 0.12889720278003125 1106 12-13 0.14272263915523808 1106 14-15 0.3307748956737484 1106 16-17 0.3561264441141745 1106 18-19 0.3714078387910149 1106 20-21 0.3844134052608297 1106 22-23 0.4051078198256306 1106 24-25 0.27213231725703224 1106 26-27 0.32125995604184965 1106 28-29 0.2379453889662173 1106 30-31 0.1479078500462805 1106 32-33 0.15657982246943902 1106 34-35 0.13203324501743907 1106 36-37 0.13156767383166112 1106 38-39 0.2311221208809897 1106 40-41 0.24005100414947123 1106 42-43 0.10800529001448922 1106 44-45 0.1252510393811903 1106 46-47 0.3111669380993263 1106 48-49 0.32191653725735847 1106 50-51 0.2013214870174025 1106 52-53 0.21791673875253537 1106 54-55 0.21359426845653928 1106 56-57 0.2738447195283058 1106 58-59 0.2820774041899483 1106 60-61 0.2793593531497649 1106 62-63 0.3275218957028443 1106 64-65 0.13268613252394346 1106 66-67 0.18871084547875938 1106 68-69 0.20097568771233654 1106 70-71 0.08793402293871821 1106 72-73 0.1165218681424065 1106 74-75 -0.02780925108853438 1106 76-77 0.09244884857756475 1106 78-79 0.19831803838392048 1106 80-81 0.2388922441812653 1106 82-83 0.29714652132397745 1106 84-85 0.3967150582995629 1106 86-87 0.3200567660875855 1106 88-89 0.5262403909812825 1106 90-91 0.4458566581534349 1106 92-93 0.3707480863347179 1106 94-95 0.3860855949183666 1106 96-97 0.48775804905448084 1106 98-99 0.4518510990350677 1106 100-101 0.3251917403031648 1107 1 -0.012611099393630809 1107 2 0.07458007013750034 1107 3 -0.051767648142515554 1107 4 0.001025515362044871 1107 5 0.06639828930880753 1107 6 0.044778577632399674 1107 7 0.01217149619162683 1107 8 0.1300447983872175 1107 9 0.06428887838431052 1107 10-11 0.17199994638784943 1107 12-13 0.21868394939266267 1107 14-15 0.24463071457820007 1107 16-17 0.2243817112938089 1107 18-19 0.19798887064719395 1107 20-21 0.2021568193184322 1107 22-23 0.1692537907571463 1107 24-25 0.15014524815580188 1107 26-27 0.1738095034967202 1107 28-29 0.05618137120833211 1107 30-31 0.2225853647173608 1107 32-33 0.16499753118876725 1107 34-35 0.13282023395145615 1107 36-37 0.14008619705957415 1107 38-39 0.25247526956331967 1107 40-41 0.09205757616554422 1107 42-43 0.21819327728461957 1107 44-45 0.0920523373150175 1107 46-47 0.3037934012720527 1107 48-49 0.2696784248924686 1107 50-51 0.20037343706668054 1107 52-53 0.2643036739188176 1107 54-55 0.3878604720425045 1107 56-57 0.35849605875671386 1107 58-59 0.26866180863959244 1107 60-61 0.1864524258306659 1107 62-63 0.24129355114368423 1107 64-65 0.2094588027702855 1107 66-67 0.12371818404839274 1107 68-69 0.27788625888556595 1107 70-71 0.20262611787025975 1107 72-73 0.2672746770223213 1107 74-75 0.33282407594767704 1107 76-77 0.3540316181973431 1107 78-79 0.3873372191767288 1107 80-81 0.2753058089096285 1107 82-83 0.16448900849359305 1107 84-85 0.17035212485772533 1107 86-87 0.18524627314399922 1107 88-89 0.31214579609212123 1107 90-91 0.13715052306078235 1107 92-93 0.19718122663702786 1107 94-95 0.17668144802103214 1107 96-97 0.3304323158635576 1107 98-99 0.3320718071222686 1107 100-101 0.20163886748324344 1108 1 -0.07169778526188253 1108 2 0.09909228792064795 1108 3 0.008487392273728744 1108 4 -0.034970489469316135 1108 5 0.13293642923262183 1108 6 0.0752394156887064 1108 7 0.17366921987461126 1108 8 0.14550284911656775 1108 9 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-0.8048639022916966 2316 98-99 -0.9173411128631201 2316 100-101 -0.7971383614900027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5433.0 3 1206.0 4 1176.0 5 1291.0 6 1538.0 7 1776.0 8 2245.0 9 2772.0 10 3523.0 11 4612.0 12 6798.0 13 11417.0 14 10003.0 15 10730.0 16 14075.0 17 20072.0 18 19093.0 19 10869.0 20 10020.0 21 10253.0 22 10801.0 23 11704.0 24 13119.0 25 14924.0 26 17175.0 27 19848.0 28 23478.0 29 27953.0 30 33974.0 31 41808.0 32 52812.0 33 69430.0 34 97401.0 35 148768.0 36 236404.0 37 400522.0 38 445848.0 39 94040.0 40 70.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.23294182945456 19.06865316428551 14.104704248656297 21.59370075760363 2 14.898876263063324 21.556647859046695 40.47666250234702 23.06781337554296 3 20.904908273070973 25.91843616612326 29.726279774344572 23.450375786461198 4 12.156447482218898 16.63239205332469 38.613249668661254 32.59791079579516 5 12.6837446506714 38.96157715885211 33.86907928074808 14.48559890972841 6 29.813502092006335 36.59217935677359 18.046621855436594 15.54769669578348 7 27.360962470105022 34.2535471526559 21.00626450833808 17.379225868900992 8 25.619228855238752 33.801098998484015 20.16005168302824 20.419620463248993 9 26.431889681208055 17.890834731543624 21.70537961409396 33.97189597315437 10-11 24.87455029876966 26.78296395547804 27.0249892711373 21.317496474614998 12-13 26.897479255967593 24.78925636084785 27.40702872048973 20.906235662694826 14-15 23.16198128574204 25.781563170910815 27.790025511815898 23.26643003153125 16-17 20.887772264561672 29.178970039888096 28.26486785757727 21.668389837972963 18-19 20.57522709748091 28.443194233204018 30.646066671892875 20.335511997422202 20-21 22.281000394884437 26.409168450988446 30.20215670881389 21.107674445313226 22-23 21.25352467607594 26.763439396811982 30.318389729461693 21.66464619765038 24-25 21.761931575437153 26.74104343406342 30.585725566880473 20.911299423618956 26-27 21.537934995554647 26.279173452715337 31.472185723989437 20.71070582774058 28-29 20.44139186214077 27.898556710763895 30.864937656775933 20.795113770319407 30-31 20.972454763219 26.972515074556615 30.611334073676854 21.443696088547533 32-33 20.92401457769751 27.06751401696641 30.71560930113528 21.2928621042008 34-35 21.841940671908507 26.7804208888702 30.56269183870832 20.81494660051297 36-37 21.59581199568755 26.437948511699204 31.126264466081565 20.83997502653168 38-39 21.191976998947943 27.240278997751155 30.922952475334025 20.644791527966877 40-41 21.162879044983697 27.457034421396525 30.566162229227274 20.813924304392508 42-43 20.63644703827746 27.68714099914153 29.793603948858117 21.882808013722894 44-45 21.341929452586943 27.066511139231448 29.775046689813305 21.81651271836831 46-47 21.888293063108662 27.72816657362098 29.259827506199315 21.123712857071038 48-49 21.679715932019747 27.545501761209895 29.403742067579604 21.371040239190755 50-51 20.825993082331625 28.014424925715737 29.93624806113406 21.223333930818576 52-53 21.438774991820416 28.541233394391767 28.694796363237224 21.325195250550593 54-55 20.69525282100207 27.724709675296904 29.323314800802713 22.256722702898312 56-57 21.606234562849906 27.714945253126505 28.76699352794152 21.91182665608207 58-59 22.354989119317107 27.444944165470563 28.72045168513787 21.47961503007446 60-61 21.521114997678055 27.695313136859223 29.86734657921628 20.916225286246444 62-63 21.22213605528461 27.72477794762635 29.654508005937714 21.398577991151324 64-65 20.836428555258333 28.514983155499564 29.135931617487955 21.51265667175415 66-67 21.176822423608055 27.800134155818718 28.575663249940504 22.447380170632723 68-69 20.892508280543225 27.954850634271178 28.944829728861578 22.20781135632402 70-71 21.602441259625518 27.769749473918658 28.698622329016654 21.92918693743917 72-73 21.465257258867855 27.212646340924074 29.534804868237885 21.787291531970187 74-75 20.6759851590026 28.738412546134594 28.736069452455865 21.84953284240694 76-77 21.011630854283077 27.651080162061813 28.77110492457672 22.56618405907839 78-79 21.152250054603392 27.76123448408344 29.031427872204375 22.055087589108794 80-81 21.422110948159055 27.724803007978625 28.770795575926172 22.08229046793615 82-83 21.175179719218495 27.514081975229622 29.25703072320898 22.053707582342906 84-85 20.935500010395966 27.81310212706712 29.030219623318647 22.221178239218265 86-87 20.83280474137772 27.90952365661754 28.88454551532057 22.373126086684167 88-89 20.826778866503673 27.70604716689094 28.4826330319858 22.984540934619588 90-91 20.96640986197417 27.180455076905822 28.871322183044363 22.981812878075647 92-93 21.43357700051387 27.578644266976678 28.91386459939178 22.073914133117675 94-95 20.98819255222525 27.25403783122063 29.05377193328858 22.70399768326554 96-97 20.337626123415216 27.159202856313954 29.709998415148025 22.79317260512281 98-99 20.84211817622055 27.38025257701156 28.575193034006784 23.202436212761107 100-101 20.716002803745944 26.47721114138939 29.421328631294514 23.385457423570156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3548.0 1 2152.0 2 1031.5 3 1316.5 4 1086.0 5 1021.5 6 1774.0 7 3481.5 8 5271.0 9 5752.0 10 4774.0 11 3899.0 12 3836.5 13 4004.0 14 4215.5 15 4314.5 16 4424.0 17 4476.0 18 4467.5 19 4676.0 20 5107.5 21 5712.5 22 6589.5 23 7707.0 24 9022.5 25 10868.0 26 13660.0 27 16835.5 28 19021.0 29 21306.0 30 24684.5 31 28514.5 32 32638.0 33 36636.5 34 40802.0 35 43755.0 36 47540.0 37 52181.0 38 53597.0 39 53986.5 40 54621.5 41 56158.0 42 58431.0 43 60355.0 44 62907.0 45 65101.5 46 67577.0 47 79331.5 48 108513.0 49 106468.5 50 76409.5 51 63395.0 52 56999.5 53 53524.0 54 50481.0 55 46131.5 56 41102.5 57 36037.5 58 32010.0 59 27959.0 60 23532.0 61 19644.5 62 15886.5 63 11899.5 64 8618.0 65 6629.0 66 4998.0 67 3894.5 68 2895.0 69 2029.0 70 1530.0 71 1231.5 72 1049.0 73 931.0 74 751.0 75 604.5 76 475.0 77 286.0 78 180.5 79 125.5 80 82.5 81 62.0 82 56.5 83 41.0 84 21.5 85 16.0 86 14.0 87 12.5 88 9.5 89 7.0 90 7.0 91 7.5 92 7.0 93 6.0 94 3.5 95 2.5 96 3.5 97 2.5 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33541454838995255 2 0.12084981463932852 3 0.11419705067782235 4 0.12163557416234105 5 0.13970804319162947 6 0.09015280927363865 7 0.09890093196317826 8 0.10330118529204849 9 0.09329584736568881 10-11 0.09127906458995663 12-13 0.09460544657070971 14-15 0.09562693395062602 16-17 0.08617162769037512 18-19 0.10086533077070961 20-21 0.10969202941255046 22-23 0.11762820059497711 24-25 0.13182425597740363 26-27 0.1307503846292865 28-29 0.125642947729705 30-31 0.11568999377154618 32-33 0.10977060536485173 34-35 0.33216674236150073 36-37 0.49057586220082855 38-39 0.46590301317823485 40-41 0.44253976336066203 42-43 0.4761702709455988 44-45 0.4824039631614982 46-47 0.49117827783513823 48-49 0.5016550714753054 50-51 0.48992106259831814 52-53 0.4942165479907867 54-55 0.48028241244936437 56-57 0.4576001542184024 58-59 0.43819189399999264 60-61 0.3960227995983197 62-63 0.46739595627195873 64-65 0.5001621283815816 66-67 0.5079673396435062 68-69 0.5212728675665185 70-71 0.5132319284476902 72-73 0.5054529091698661 74-75 0.5124461689246776 76-77 0.48903053513890393 78-79 0.46705546047865326 80-81 0.4697532348409963 82-83 0.48256111506610067 84-85 0.48222061927279525 86-87 0.48612322490375753 88-89 0.4983286894945523 90-91 0.5137557681296985 92-93 0.5065005885338827 94-95 0.5110579937673555 96-97 0.5107436899581504 98-99 0.504955261471958 100-101 0.4913354297397407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1908981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.50822515462637 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8735185311145 39.31475569150851 2 14.192139862431405 14.904081506449929 3 4.95119459652187 7.799353219756195 4 2.171463629451747 4.56078804681338 5 1.1194002841136481 2.938886107819609 6 0.6273214827439864 1.9763722596153184 7 0.42377422960072214 1.5576142863822136 8 0.2902341788932128 1.2191745290314344 9 0.18873383229961693 0.8919070704615404 >10 1.0255488352394588 9.841605135394042 >50 0.08494882033834905 3.048328566999199 >100 0.045831564158180654 4.315639152176447 >500 0.00219632827207743 0.7067344031047733 >1k 0.0029949930982874047 3.364038984189714 >5k 4.991655163812341E-4 2.0148902427042112 >10k+ 1.9966620655249365E-4 1.5458307975935812 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17051 0.8931990417924536 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12438 0.6515517964819975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9697 0.5079673396435062 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9203 0.4820896593522932 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6599 0.3456818061573164 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6522 0.341648240605852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6416 0.33609553997656344 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4499 0.23567547293556093 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3780 0.19801139979915985 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3609 0.18905374123681692 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3513 0.18402488028953667 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3513 0.18402488028953667 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3079 0.16129023809037388 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3075 0.1610807022175705 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3041 0.1592996472987421 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2646 0.13860797985941192 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2503 0.13111707240669235 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2301 0.12053551083012351 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2272 0.11901637575229926 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2117 0.11089686068116969 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.238396820083594E-5 0.0 10-11 0.0 0.0 0.0 1.0476793640167188E-4 0.0 12-13 0.0 0.0 0.0 2.0953587280334376E-4 0.0 14-15 0.0 0.0 0.0 2.0953587280334376E-4 0.0 16-17 0.0 0.0 0.0 3.666877774058516E-4 0.0 18-19 0.0 0.0 0.0 4.1907174560668753E-4 0.0 20-21 0.0 0.0 0.0 6.547996025104493E-4 0.0 22-23 0.0 0.0 0.0 0.0011262553163179727 0.0 24-25 0.0 0.0 0.0 0.001728670950627586 0.0 26-27 0.0 0.0 0.0 0.0023572785690376174 0.0 28-29 0.0 0.0 0.0 0.006233692215899477 0.0 30-31 0.0 0.0 0.0 0.01652714196736374 0.0 32-33 0.0 0.0 0.0 0.035254410599162594 0.0 34-35 0.0 0.0 0.0 0.06511327247363907 0.0 36-37 0.0 0.0 0.0 0.11173500417238307 0.0 38-39 0.0 0.0 0.0 0.18020085061087565 0.0 40-41 0.0 0.0 0.0 0.2597197143397446 0.0 42-43 0.0 0.0 0.0 0.33837424259329973 0.0 44-45 0.0 5.238396820083594E-5 0.0 0.4323510815455994 0.0 46-47 0.0 5.238396820083594E-5 0.0 0.5487744508719574 0.0 48-49 0.0 5.238396820083594E-5 0.0 0.6735530631263486 0.0 50-51 0.0 5.238396820083594E-5 0.0 0.8137849459999864 0.0 52-53 0.0 5.238396820083594E-5 0.0 0.9495903835606536 0.0 54-55 0.0 5.238396820083594E-5 0.0 1.1036516340393119 5.238396820083594E-5 56-57 0.0 5.238396820083594E-5 0.0 1.291762463848514 5.238396820083594E-5 58-59 0.0 5.238396820083594E-5 0.0 1.5067462693447446 5.238396820083594E-5 60-61 0.0 5.238396820083594E-5 0.0 1.7458790841815608 5.238396820083594E-5 62-63 0.0 5.238396820083594E-5 0.0 1.9792496625162848 5.238396820083594E-5 64-65 0.0 5.238396820083594E-5 0.0 2.213091696564817 5.238396820083594E-5 66-67 0.0 5.238396820083594E-5 0.0 2.4897052406493305 5.238396820083594E-5 68-69 0.0 5.238396820083594E-5 0.0 2.816083554524639 5.238396820083594E-5 70-71 0.0 5.238396820083594E-5 0.0 3.1729493378928337 5.238396820083594E-5 72-73 0.0 5.238396820083594E-5 0.0 3.535210669985715 5.238396820083594E-5 74-75 0.0 5.238396820083594E-5 0.0 3.893883700256839 5.238396820083594E-5 76-77 0.0 5.238396820083594E-5 0.0 4.27874871462838 5.238396820083594E-5 78-79 0.0 5.238396820083594E-5 0.0 4.752666474941343 5.238396820083594E-5 80-81 0.0 5.238396820083594E-5 0.0 5.269250977353886 5.238396820083594E-5 82-83 0.0 5.238396820083594E-5 0.0 5.773734783112037 5.238396820083594E-5 84-85 0.0 5.238396820083594E-5 0.0 6.293252787743828 5.238396820083594E-5 86-87 0.0 5.238396820083594E-5 0.0 6.840036647824153 5.238396820083594E-5 88-89 0.0 5.238396820083594E-5 0.0 7.432158832382303 5.238396820083594E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12700 0.0 24.560284 1 TACATGG 12800 0.0 23.802223 2 GAGTACT 9005 0.0 23.167511 12-13 CATGGGG 3805 0.0 22.912878 4 ACATGGG 12795 0.0 22.885725 3 GTAAGCG 235 0.0 21.248865 94-95 GTACTTT 9815 0.0 21.232836 14-15 AGTACTT 9360 0.0 20.391369 12-13 GTATTAC 460 0.0 19.609545 1 TACCTGG 1345 0.0 19.375677 2 CATGGGA 8710 0.0 19.094376 4 TAGGACG 125 0.006119599 18.952936 4 ACTTTTT 11400 0.0 18.469868 16-17 ATGGGAG 3275 0.0 18.229542 5 AGTACAT 9745 0.0 17.747103 2 TACTTTT 10965 0.0 17.407726 14-15 ATGGGGG 2020 0.0 16.888756 5 GTGTAGC 1020 0.0 16.756144 1 TACCCGG 200 2.8142644E-4 16.583818 2 GTGTAAG 870 0.0 16.370945 1 >>END_MODULE