FastQCFastQC Report
Fri 27 May 2016
SRR939201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908981
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT183470.961088664580737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124660.6530185475916208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102590.5374071297723759No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88550.4638600384184023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68160.3570491272568978No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62450.32713788141422045No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC59610.31226083444518304No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48670.25495277323346854No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39420.20649760264769532No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39230.2055023072518794No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG38370.20099728598660752No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38370.20099728598660752No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31880.167000090624265No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31740.16626671506945329No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29630.1552136977790769No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27600.1445797522343072No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26870.14075572255564617No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.13368388684853333No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.12168795813054188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22740.11912114368870094No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA459.01081E-426.3589948-49
TCGCGAT500.001665425623.7227850-51
TACATGG124600.022.7327442
GTACATG125650.022.6985591
ACATGGG124550.021.9419043
AGTACTT102250.021.53212-13
GAGTACT99450.021.25556412-13
CATGGGG35450.021.012564
GTACTTT106450.019.8355414-15
CATGGGA84650.019.3369374
TAAGGTG6700.019.119855
ATGGGAG28850.019.0768995
TACCTGG13250.018.6201442
ATGGGAT32700.017.9915895
TACTTTT122300.017.84682814-15
AGTACAT97400.017.7311952
AGAGTAC204850.016.94369110-11
ACTTTTT125750.016.90351316-17
GTAAGCG2257.2759576E-1216.87041594-95
ATGGGGA19150.016.5997525
Produced by FastQC (version 0.11.5)