##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939201_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1908981 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49813644033126 34.0 31.0 34.0 31.0 34.0 2 32.688358867898636 34.0 31.0 34.0 31.0 34.0 3 32.778520058607185 34.0 31.0 34.0 31.0 34.0 4 36.22881317310125 37.0 35.0 37.0 35.0 37.0 5 36.20232155270273 37.0 35.0 37.0 35.0 37.0 6 36.26063905298167 37.0 37.0 37.0 35.0 37.0 7 36.21763652964592 37.0 37.0 37.0 35.0 37.0 8 36.20961130571755 37.0 37.0 37.0 35.0 37.0 9 37.94184593770184 39.0 38.0 39.0 35.0 39.0 10-11 37.88507271680545 39.0 38.0 39.0 35.0 39.0 12-13 37.82581178125922 39.0 38.0 39.0 35.0 39.0 14-15 39.1352716449247 40.0 38.5 41.0 36.0 41.0 16-17 39.10493294590151 40.0 38.0 41.0 36.0 41.0 18-19 39.17933206249826 40.5 38.5 41.0 36.0 41.0 20-21 39.258859045742206 41.0 39.0 41.0 36.0 41.0 22-23 39.213976199867886 41.0 39.0 41.0 36.0 41.0 24-25 39.1517817621024 41.0 39.0 41.0 35.0 41.0 26-27 38.99556753053069 40.5 38.5 41.0 35.0 41.0 28-29 38.85334138998765 40.0 38.0 41.0 35.0 41.0 30-31 38.65802802647067 40.0 38.0 41.0 35.0 41.0 32-33 38.47330984436199 40.0 38.0 41.0 34.0 41.0 34-35 38.383127961986006 40.0 38.0 41.0 34.0 41.0 36-37 38.25540432303936 40.0 38.0 41.0 34.0 41.0 38-39 38.112306775185296 40.0 38.0 41.0 34.0 41.0 40-41 37.95581883737973 40.0 38.0 41.0 33.0 41.0 42-43 37.76460687665305 40.0 37.0 41.0 33.0 41.0 44-45 37.49684281823654 40.0 37.0 41.0 33.0 41.0 46-47 37.131951025180456 40.0 37.0 41.0 32.0 41.0 48-49 36.75631632792573 40.0 37.0 41.0 31.0 41.0 50-51 36.49047999953902 40.0 36.0 41.0 30.5 41.0 52-53 36.28514113026793 40.0 36.0 41.0 30.0 41.0 54-55 36.07003998468293 39.0 35.0 41.0 29.5 41.0 56-57 35.807511180048415 39.0 35.0 41.0 28.5 41.0 58-59 35.533634436382556 39.0 35.0 40.0 28.0 41.0 60-61 35.15738868013878 38.0 35.0 40.0 28.0 41.0 62-63 34.81410789316394 38.0 34.5 40.0 27.0 41.0 64-65 34.42815355417367 37.0 34.0 40.0 26.0 41.0 66-67 34.060681326844005 37.0 34.0 39.5 26.0 41.0 68-69 33.60004185479059 36.0 33.5 39.0 25.5 40.5 70-71 33.16955171371533 36.0 33.0 39.0 24.5 40.0 72-73 32.70768069456951 35.0 33.0 38.0 23.0 40.0 74-75 32.23020318169746 35.0 32.0 37.0 21.5 39.0 76-77 30.250939899349444 33.5 29.5 35.5 18.0 37.5 78-79 31.20506201999915 34.5 31.0 36.0 19.5 39.0 80-81 31.220137078367987 35.0 32.0 36.0 19.5 37.5 82-83 30.920211096915054 35.0 31.5 35.5 18.0 37.0 84-85 30.54718957391404 34.5 31.0 35.0 13.0 37.0 86-87 30.042044420557353 34.0 31.0 35.0 6.0 36.0 88-89 29.49924462317854 34.0 30.0 35.0 2.0 36.0 90-91 29.057261177560175 34.0 29.5 35.0 2.0 35.5 92-93 28.8056656928487 34.0 29.0 35.0 2.0 35.0 94-95 28.49814796480426 34.0 29.0 35.0 2.0 35.0 96-97 27.97117284037924 34.0 29.0 35.0 2.0 35.0 98-99 27.28660002378232 34.0 28.0 35.0 2.0 35.0 100-101 23.80102028254865 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13996543470762646 1101 2 0.21052029275216455 1101 3 0.29813075750335827 1101 4 0.16484079943742813 1101 5 0.1972552252654225 1101 6 0.19255603929682508 1101 7 0.19463370640757915 1101 8 0.195888874968432 1101 9 0.29839560612821003 1101 10-11 0.3116252340561516 1101 12-13 0.29345641629603136 1101 14-15 0.40740228443016946 1101 16-17 0.3568311804239812 1101 18-19 0.33599489114360637 1101 20-21 0.40919715218088015 1101 22-23 0.3947722394733688 1101 24-25 0.34772027576222797 1101 26-27 0.47290518606456544 1101 28-29 0.4748236152883294 1101 30-31 0.47927577873718974 1101 32-33 0.38870117691535455 1101 34-35 0.6243331368354816 1101 36-37 0.6057990975766643 1101 38-39 0.5430349075626069 1101 40-41 0.6266466855882555 1101 42-43 0.5848181820514426 1101 44-45 0.7483638388686131 1101 46-47 0.686859769213136 1101 48-49 0.7728057602551175 1101 50-51 0.7939689141365633 1101 52-53 0.9125622211874642 1101 54-55 0.88156468267006 1101 56-57 0.7229673802111662 1101 58-59 0.8029335403975963 1101 60-61 0.7557712214574508 1101 62-63 0.5560205780875975 1101 64-65 0.8064364383723301 1101 66-67 0.8999234367779891 1101 68-69 0.7917526091367293 1101 70-71 0.7724941930799645 1101 72-73 0.6716569390542517 1101 74-75 0.6413310752213093 1101 76-77 0.6727582754231882 1101 78-79 0.6269486926323431 1101 80-81 0.5955800437303225 1101 82-83 0.7856646660139432 1101 84-85 0.6723797457373486 1101 86-87 0.6546370163554975 1101 88-89 0.9299280383394368 1101 90-91 0.9062411677194788 1101 92-93 0.847162334819604 1101 94-95 0.7616236303871595 1101 96-97 0.9203410961801737 1101 98-99 0.9831984989132359 1101 100-101 0.3593236703266065 1102 1 0.13612345140350612 1102 2 0.07786009252044579 1102 3 0.12628425858770242 1102 4 0.09857345740956447 1102 5 0.045109567085333424 1102 6 0.06896642520336371 1102 7 0.07657942994520539 1102 8 0.057129628362567075 1102 9 0.12086374432968938 1102 10-11 0.13566752335237453 1102 12-13 0.12276147259263581 1102 14-15 0.1679712663380073 1102 16-17 0.2286416584252251 1102 18-19 0.15475807405558584 1102 20-21 0.16383939394434321 1102 22-23 0.15739664160046374 1102 24-25 0.17381798527768666 1102 26-27 0.09319424461897796 1102 28-29 0.025847426226803805 1102 30-31 0.09569574546426196 1102 32-33 0.042904483412485206 1102 34-35 0.03019043459846671 1102 36-37 0.13791229979881336 1102 38-39 0.14695487428966914 1102 40-41 0.07474574681638302 1102 42-43 0.05664722753434859 1102 44-45 0.16702898547744383 1102 46-47 0.19434357836912852 1102 48-49 0.25718530928811845 1102 50-51 0.42664025485728274 1102 52-53 0.48229871148780745 1102 54-55 0.3730288400034709 1102 56-57 0.4460801813757129 1102 58-59 0.34175740183962233 1102 60-61 0.3535691181331373 1102 62-63 0.3627642874248167 1102 64-65 0.16965611396129532 1102 66-67 0.3231639822163359 1102 68-69 0.3426768983735329 1102 70-71 0.2472294207915624 1102 72-73 0.22075753024287081 1102 74-75 0.28819168542303686 1102 76-77 0.3978128638192544 1102 78-79 0.242489674777854 1102 80-81 0.28087868310526787 1102 82-83 0.2822972675409332 1102 84-85 0.3425599988492607 1102 86-87 0.3671685810743135 1102 88-89 0.4217174979514482 1102 90-91 0.5155130253588851 1102 92-93 0.3197905247129498 1102 94-95 0.3811967268489447 1102 96-97 0.43561470658984547 1102 98-99 0.060129888355024974 1102 100-101 -0.22773182416256432 1103 1 0.13981900582398055 1103 2 0.09518168506501468 1103 3 0.16735228036375105 1103 4 0.10881536015077842 1103 5 0.09988730266146462 1103 6 0.14065610631899972 1103 7 0.12452195421479217 1103 8 0.21122602565176862 1103 9 0.13016120335407777 1103 10-11 0.26312849774470237 1103 12-13 0.2604345175286582 1103 14-15 0.26464395765334814 1103 16-17 0.2816924008103783 1103 18-19 0.23740199258021022 1103 20-21 0.25960547750069907 1103 22-23 0.2355115232102989 1103 24-25 0.17190872463596207 1103 26-27 0.3368530836080552 1103 28-29 0.2799482619649041 1103 30-31 0.26198005067237773 1103 32-33 0.3369873761752018 1103 34-35 0.4843096062238246 1103 36-37 0.47103680774945644 1103 38-39 0.47900978881678924 1103 40-41 0.5249365273576387 1103 42-43 0.47199618420781064 1103 44-45 0.5468219645788963 1103 46-47 0.7184625462624084 1103 48-49 0.7130511196979583 1103 50-51 0.7918110914830621 1103 52-53 0.878586349170682 1103 54-55 0.8276402428880303 1103 56-57 0.8591506130112307 1103 58-59 0.9512186469045716 1103 60-61 0.8420498880075655 1103 62-63 0.9487588710619264 1103 64-65 0.8974842426675735 1103 66-67 0.8944494734362038 1103 68-69 0.7188736424438389 1103 70-71 0.6047875899386597 1103 72-73 0.5931586728887979 1103 74-75 0.5630557306399098 1103 76-77 0.6725634011626163 1103 78-79 0.5398584749559738 1103 80-81 0.5620831617184905 1103 82-83 0.5255108064108285 1103 84-85 0.6478346951793164 1103 86-87 0.613780443811283 1103 88-89 0.531368286612885 1103 90-91 0.7618054652979396 1103 92-93 0.6383470974736838 1103 94-95 0.44935565824507506 1103 96-97 0.42532769174505347 1103 98-99 0.461372217478381 1103 100-101 0.08807691216009772 1104 1 0.07732017867391505 1104 2 0.007956053758988446 1104 3 0.11442390865476426 1104 4 -0.008535312595945754 1104 5 0.09980598506621163 1104 6 0.07684994244527843 1104 7 0.06177604667914949 1104 8 0.0828662015479793 1104 9 0.14944911142766415 1104 10-11 0.11605155253399602 1104 12-13 0.07379187082858607 1104 14-15 0.04948698341851809 1104 16-17 0.10581651648153212 1104 18-19 0.0677641060870613 1104 20-21 0.16957595295841799 1104 22-23 0.11920859640238035 1104 24-25 0.09851803112197643 1104 26-27 0.07940783224285752 1104 28-29 0.08774005138986496 1104 30-31 0.15474559953486988 1104 32-33 0.13999393787961623 1104 34-35 0.05350618838274812 1104 36-37 -0.04860451910349184 1104 38-39 0.010833116958309574 1104 40-41 0.008122550739578571 1104 42-43 -0.08847439446071093 1104 44-45 -0.04005806694731007 1104 46-47 0.004132822179265361 1104 48-49 0.11755455067496001 1104 50-51 0.1151908383976874 1104 52-53 -0.02865998684614368 1104 54-55 -0.06208058755228052 1104 56-57 0.07382437307428091 1104 58-59 -0.11194323140676943 1104 60-61 -0.026181204087741605 1104 62-63 -0.20148946036356818 1104 64-65 -0.12139082223011854 1104 66-67 -0.04861726328869764 1104 68-69 0.08445096609412417 1104 70-71 0.146026557330309 1104 72-73 0.00549361380843294 1104 74-75 -0.08831853671983225 1104 76-77 0.04306982212504096 1104 78-79 0.07350050239127981 1104 80-81 0.02041102175214249 1104 82-83 -0.003984023666671277 1104 84-85 -0.06745676584766258 1104 86-87 -0.07408040301764274 1104 88-89 0.02691614827289257 1104 90-91 0.1808272146270653 1104 92-93 0.1385769760602571 1104 94-95 0.09547555791167284 1104 96-97 -0.025190551544682194 1104 98-99 0.015596979311656156 1104 100-101 -0.060444984166220905 1105 1 0.1353237933744964 1105 2 0.1850656899867218 1105 3 0.139208565323365 1105 4 0.04941617663864406 1105 5 0.15959289215156502 1105 6 0.10170343146000249 1105 7 0.18045871586387818 1105 8 0.08011892217987082 1105 9 0.085518229795575 1105 10-11 0.12332004780210326 1105 12-13 0.1345273945675629 1105 14-15 0.19611023892785795 1105 16-17 0.21418135063733956 1105 18-19 0.20871855010047113 1105 20-21 0.18658907019828064 1105 22-23 0.2088607224030028 1105 24-25 0.15392255648118436 1105 26-27 0.17034287956844452 1105 28-29 0.1581638949145585 1105 30-31 0.2532045903257085 1105 32-33 0.1346474021013293 1105 34-35 0.2046565072001414 1105 36-37 0.16760773713060217 1105 38-39 0.10371702185071996 1105 40-41 0.07628612045187566 1105 42-43 0.002845913520602039 1105 44-45 0.06894547123576444 1105 46-47 0.04732211213352144 1105 48-49 0.0754976530557201 1105 50-51 0.10815055911211147 1105 52-53 0.1283115102139547 1105 54-55 0.0738101681633978 1105 56-57 0.03111590713009349 1105 58-59 0.014342738923815546 1105 60-61 0.10513905043155347 1105 62-63 0.08057262975400903 1105 64-65 -0.006292614176665268 1105 66-67 -0.0403239679935794 1105 68-69 -0.06324854663301238 1105 70-71 0.07707191684399106 1105 72-73 -0.006275213759522558 1105 74-75 -0.08938192963992009 1105 76-77 0.07712445830098602 1105 78-79 0.08101174862763472 1105 80-81 -0.009159487797678167 1105 82-83 -0.06713720002884216 1105 84-85 0.06584141620988504 1105 86-87 0.07340776767588153 1105 88-89 0.19639775717217844 1105 90-91 0.27050406681027184 1105 92-93 0.21853059107827022 1105 94-95 0.0014976865085394309 1105 96-97 0.08145714947166738 1105 98-99 0.3428927803046484 1105 100-101 0.12441347013327686 1106 1 0.0419922058072828 1106 2 -0.004114191779038379 1106 3 0.07571587986040385 1106 4 0.0067184965005893105 1106 5 0.02416126262862406 1106 6 0.102632064349919 1106 7 0.06880040689321731 1106 8 0.06739266945729128 1106 9 0.10408260476192766 1106 10-11 0.12058878396619122 1106 12-13 0.10465472399457809 1106 14-15 0.0073040403112827335 1106 16-17 -0.0075373178783095796 1106 18-19 0.011033952058340901 1106 20-21 0.10539167215436862 1106 22-23 0.12684953611988448 1106 24-25 0.1481217069811933 1106 26-27 0.13246851547489769 1106 28-29 0.10178393210447467 1106 30-31 0.21578116271781766 1106 32-33 0.1063656724039106 1106 34-35 0.08594775610146854 1106 36-37 0.18718293616546333 1106 38-39 0.2576115616486092 1106 40-41 0.22229874922203408 1106 42-43 0.18677511697021032 1106 44-45 0.10750189468343763 1106 46-47 0.241771467872546 1106 48-49 0.21921418523786684 1106 50-51 0.08777192537798584 1106 52-53 0.10937106281996734 1106 54-55 0.134869134599775 1106 56-57 0.017961495709720054 1106 58-59 0.010795775425940235 1106 60-61 -0.01718611252520219 1106 62-63 -0.011858836979435239 1106 64-65 0.0017479449399786517 1106 66-67 -0.0064439996085141615 1106 68-69 -0.12527947154180907 1106 70-71 -0.20601807644372627 1106 72-73 -0.2168408897676315 1106 74-75 -0.06896984600752631 1106 76-77 0.004555381871789166 1106 78-79 0.05944500636419647 1106 80-81 0.11234089623901511 1106 82-83 0.11947755239571833 1106 84-85 0.056479586111795044 1106 86-87 0.3434121607625187 1106 88-89 0.3539384951661333 1106 90-91 0.3598395065314861 1106 92-93 0.39196114574448515 1106 94-95 0.2971923978884803 1106 96-97 0.3435026967645669 1106 98-99 0.6801975722733573 1106 100-101 0.20664622217109496 1107 1 -0.08963036889232967 1107 2 -0.08450846430396552 1107 3 -0.05967891338811171 1107 4 0.008807143030992393 1107 5 0.0072855799357824935 1107 6 0.08356147118520596 1107 7 0.028077097610051283 1107 8 -0.002199313337030162 1107 9 0.04855803024153715 1107 10-11 0.03074867638228085 1107 12-13 0.005800648908660833 1107 14-15 0.04987485871922814 1107 16-17 0.13970741857500713 1107 18-19 0.020371555325091606 1107 20-21 0.01823964871938699 1107 22-23 0.02359852555282771 1107 24-25 -0.04860388016491868 1107 26-27 0.039993001593380484 1107 28-29 -0.05165817190945177 1107 30-31 0.05815798131466465 1107 32-33 0.07067476302675857 1107 34-35 0.0558820238308968 1107 36-37 0.08509401716341358 1107 38-39 0.1271572848125757 1107 40-41 0.03890171807307752 1107 42-43 0.07327675058909477 1107 44-45 -0.0013499219152919295 1107 46-47 0.017748308993724038 1107 48-49 0.07068625871075795 1107 50-51 -0.05087345459279646 1107 52-53 -0.11407400205249729 1107 54-55 -0.08733376231875667 1107 56-57 -0.08668487242490386 1107 58-59 0.04986745242230484 1107 60-61 -0.04686084798551349 1107 62-63 -0.07409711282730314 1107 64-65 0.028076123109784135 1107 66-67 -0.01833329982867582 1107 68-69 -0.15732077461020566 1107 70-71 -0.048213550023668006 1107 72-73 -0.15349350295235098 1107 74-75 -0.1305402635794124 1107 76-77 -0.013210677606956267 1107 78-79 0.10590944457610973 1107 80-81 -0.02887676743167944 1107 82-83 -0.05675332312227965 1107 84-85 0.12371907899967738 1107 86-87 0.07318265486865627 1107 88-89 0.20272066801245714 1107 90-91 0.17711532897200755 1107 92-93 0.1437381149878867 1107 94-95 0.014651109351905944 1107 96-97 -0.07168414612465313 1107 98-99 0.12776661053321803 1107 100-101 -0.11951459785957042 1108 1 -0.054728389890193796 1108 2 -0.031163671959824057 1108 3 -0.006993788422953173 1108 4 0.018927462644484194 1108 5 -0.007415246971220313 1108 6 0.0031424430331981057 1108 7 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90-91 -0.44513008569342816 2316 92-93 -0.2827092433905598 2316 94-95 -0.08821498597653132 2316 96-97 -0.2522594640950899 2316 98-99 -0.12558561748597796 2316 100-101 0.13010435097985606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 17.0 5 41.0 6 87.0 7 224.0 8 471.0 9 783.0 10 1480.0 11 2514.0 12 3929.0 13 6053.0 14 7047.0 15 9802.0 16 13336.0 17 20305.0 18 19742.0 19 11600.0 20 9585.0 21 9459.0 22 9880.0 23 10727.0 24 12091.0 25 13980.0 26 16583.0 27 19650.0 28 23812.0 29 29516.0 30 36185.0 31 44905.0 32 56264.0 33 72688.0 34 102315.0 35 153925.0 36 245955.0 37 442448.0 38 449764.0 39 51804.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.62136483698437 19.539429938105314 14.448664204896383 21.390541020013927 2 15.114398728955395 21.96910288787578 39.8857296117667 23.030768771402123 3 20.86987769914944 25.934726432583666 29.919417741716654 23.275978126550235 4 12.458793461014018 16.994983187365406 38.24595425517593 32.30026909644465 5 12.6957785331546 39.190751505646205 33.75465758957266 14.35881237162654 6 29.87452468096854 36.91388232779687 17.69137566062732 15.520217330607272 7 27.36061804701042 34.404690250976834 20.76023805370509 17.47445364830766 8 25.715289989790364 34.04214080705884 19.883539961895902 20.359029241254888 9 26.347459718038053 17.936218327998027 21.516610170556962 34.19971178340696 10-11 24.918346489566947 27.026041642111682 26.81920354367068 21.23640832465069 12-13 26.99091819143302 24.86266233136946 27.33054964926314 20.81586982793438 14-15 23.050688528454593 25.93283000582777 27.77199656881683 23.2444848969008 16-17 20.731429421560684 29.330908610872907 28.23739999067549 21.700261976890925 18-19 20.51663178330004 28.46825831903469 30.70054932462642 20.314560573038857 20-21 22.19257289622055 26.389078780773616 30.354545173576895 21.063803149428935 22-23 21.171373628129352 26.791099544730933 30.429506632072297 21.60802019506742 24-25 21.71570098833454 26.771178578593997 30.71458991738473 20.79853051568674 26-27 21.423804425036515 26.30555654504347 31.688525036721643 20.582113993198377 28-29 20.357536298161165 27.940534766977777 31.00832328870743 20.693605646153628 30-31 20.96949853975405 26.929737941511583 30.708566339693803 21.392197179040558 32-33 20.935410043368687 27.094847984343478 30.841716077844673 21.128025894443162 34-35 21.83494741011206 26.737949564106277 30.648327897486325 20.778775128295333 36-37 21.486086978392688 26.406838507923798 31.34037542941101 20.7666990842725 38-39 21.110049220715666 27.26997410594243 31.06406657214445 20.555910101197455 40-41 21.11819969801467 27.507253620523265 30.608392509674115 20.76615417178795 42-43 20.619052783178287 27.689282789816065 29.848027867145927 21.84363655985972 44-45 21.28924579911777 27.0759900456516 29.8444744382213 21.790289717009326 46-47 21.868025457954275 27.78506944780944 29.34791690621789 20.998988188018398 48-49 21.644091653662265 27.658254272332343 29.436439250715964 21.26121482328943 50-51 20.813785372293054 28.017449108948234 30.030016029502665 21.138749489256043 52-53 21.472472981630496 28.585358979852582 28.681797966347457 21.26037007216947 54-55 20.695360509088356 27.743675814479037 29.265299130792815 22.29566454563979 56-57 21.610875121334367 27.78673543634012 28.784807182470644 21.817582259854866 58-59 22.351527044873887 27.499459107760927 28.70013510518979 21.4488787421754 60-61 21.442435945929805 27.813344453886202 29.836078370559594 20.9081412296244 62-63 21.21570888281149 27.735058171103745 29.598938024076237 21.450294922008524 64-65 20.82196029045492 28.57766615075986 29.026764379014182 21.57360917977104 66-67 21.12522955088309 27.876874027085368 28.524946821203205 22.472949600828336 68-69 20.883954804302064 28.049474605381064 28.80802724712707 22.258543343189803 70-71 21.62455300093427 27.89894920351465 28.543324565127826 21.933173230423257 72-73 21.46946459917621 27.324106421174438 29.320223721451395 21.88620525819796 74-75 20.690122112268273 28.925458137089894 28.444704268926717 21.939715481715112 76-77 21.013879512101393 27.744219669869413 28.617634613627942 22.62426620440125 78-79 21.169750307953787 27.891537406399202 28.78898401573832 22.149728269908692 80-81 21.445903683253796 27.91577392333845 28.520543137183 22.117779256224757 82-83 21.19023809162109 27.755057567663417 28.923252369994096 22.1314519707214 84-85 20.827315724986263 28.085874086750994 28.709950491911652 22.37685969635109 86-87 20.803989979427094 28.142900669524046 28.488544358131122 22.56456499291774 88-89 20.823034660873635 27.927843104250883 28.05958019814479 23.18954203673069 90-91 20.973240795499994 27.444876080803798 28.34812183838346 23.23376128531274 92-93 21.36734205316868 27.91243076803803 28.429617686084878 22.290609492708413 94-95 20.940281752411366 27.645455874102463 28.45958131589576 22.954681057590413 96-97 20.21374759983143 27.589124142441474 29.105561843088495 23.0915664146386 98-99 20.73992023883299 27.852158439015394 27.89883333708758 23.509087985064035 100-101 20.67841057077092 26.969866346213283 28.675321981265213 23.676401101750585 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1910.0 1 1290.5 2 908.5 3 1152.0 4 963.0 5 923.0 6 1685.0 7 3350.0 8 5021.5 9 5487.0 10 4577.0 11 3634.0 12 3411.5 13 3517.0 14 3784.0 15 3883.0 16 3874.5 17 3970.0 18 4209.0 19 4493.0 20 4835.0 21 5446.0 22 6430.0 23 7703.5 24 8995.5 25 10747.5 26 13628.0 27 16849.5 28 19190.0 29 21572.0 30 24664.0 31 28459.0 32 32786.0 33 36816.5 34 40857.5 35 43851.5 36 47570.0 37 52353.5 38 53651.0 39 53467.5 40 54690.0 41 57016.5 42 58947.5 43 60237.5 44 62871.0 45 65470.0 46 67322.5 47 79564.5 48 109280.5 49 105877.0 50 76762.0 51 65190.0 52 58265.0 53 54278.5 54 51093.5 55 46852.5 56 41859.5 57 36548.0 58 32446.5 59 28392.0 60 23704.0 61 19562.0 62 15802.0 63 11776.5 64 8346.5 65 6351.5 66 4768.0 67 3647.0 68 2691.0 69 1816.5 70 1333.0 71 1107.0 72 933.0 73 886.0 74 758.5 75 595.0 76 399.0 77 219.5 78 143.5 79 89.5 80 49.5 81 29.0 82 18.5 83 13.0 84 6.5 85 3.5 86 1.5 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3434292955246805 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.691034117154649E-4 16-17 0.0017810549188284222 18-19 2.619198410041797E-5 20-21 0.0 22-23 0.0 24-25 6.286076184100313E-4 26-27 0.0013095992050208985 28-29 0.0 30-31 0.0029335022192468127 32-33 0.0 34-35 0.010555369592468443 36-37 0.028968334415062278 38-39 0.022787026167363635 40-41 0.014615127128033228 42-43 0.011000633322175548 44-45 0.00348353388535559 46-47 0.0015453270619246603 48-49 6.286076184100313E-4 50-51 5.238396820083594E-5 52-53 1.0476793640167188E-4 54-55 0.0 56-57 0.0 58-59 0.005133628883681922 60-61 0.010633945544769697 62-63 0.0045312132493723085 64-65 0.0023310865849371994 66-67 0.004740749122175653 68-69 0.004321677376568965 70-71 2.619198410041797E-5 72-73 0.0 74-75 0.0 76-77 0.2178649237472767 78-79 4.976476979079414E-4 80-81 0.003719261742259352 82-83 5.238396820083594E-4 84-85 0.0 86-87 0.11021586909455883 88-89 0.4110046145037588 90-91 0.39604899158242013 92-93 0.0 94-95 0.0 96-97 2.619198410041797E-5 98-99 0.0016762869824267501 100-101 9.952953958158829E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1908981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.47035290944287 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06045922757085 38.11917973078476 2 14.550609805088452 14.978500434310046 3 5.094357071879254 7.866250690090236 4 2.2675480451936307 4.66845992500934 5 1.1932510052180756 3.0708525174060903 6 0.6924760565179574 2.138519220619115 7 0.4310149535825753 1.55291442390996 8 0.2976968941719445 1.225805136246004 9 0.20636195365394777 0.9559370323485707 >10 1.061547410771595 9.986089783582395 >50 0.0915228087589813 3.2380156500485144 >100 0.04745315474040374 4.484306221114059 >500 0.0019344757891496312 0.6368693399666773 >1k 0.003054435456552049 3.465076482948789 >5k 4.072580608736065E-4 1.460874400580191 >10k+ 3.054435456552049E-4 2.152349011035248 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18347 0.961088664580737 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12466 0.6530185475916208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10259 0.5374071297723759 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8855 0.4638600384184023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6816 0.3570491272568978 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6245 0.32713788141422045 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5961 0.31226083444518304 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4867 0.25495277323346854 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3942 0.20649760264769532 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3923 0.2055023072518794 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3837 0.20099728598660752 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3837 0.20099728598660752 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3188 0.167000090624265 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3174 0.16626671506945329 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2963 0.1552136977790769 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2760 0.1445797522343072 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2687 0.14075572255564617 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2552 0.13368388684853333 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2323 0.12168795813054188 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2274 0.11912114368870094 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.238396820083594E-5 0.0 5 0.0 0.0 0.0 1.0476793640167188E-4 0.0 6 0.0 0.0 0.0 1.0476793640167188E-4 0.0 7 0.0 0.0 0.0 1.0476793640167188E-4 0.0 8 0.0 0.0 0.0 1.0476793640167188E-4 0.0 9 0.0 0.0 0.0 1.0476793640167188E-4 0.0 10-11 0.0 0.0 0.0 1.5715190460250782E-4 0.0 12-13 0.0 0.0 0.0 2.0953587280334376E-4 0.0 14-15 0.0 0.0 0.0 2.0953587280334376E-4 0.0 16-17 0.0 0.0 0.0 3.9287976150626956E-4 0.0 18-19 0.0 0.0 0.0 6.286076184100313E-4 0.0 20-21 0.0 0.0 0.0 8.119515071129571E-4 0.0 22-23 2.619198410041797E-5 0.0 0.0 0.0013881751573221524 0.0 24-25 5.238396820083594E-5 0.0 0.0 0.0021215507121338556 0.0 26-27 5.238396820083594E-5 0.0 0.0 0.002802542298744723 0.0 28-29 5.238396820083594E-5 0.0 0.0 0.006809915866108672 0.0 30-31 5.238396820083594E-5 0.0 0.0 0.01718194156987419 0.0 32-33 5.238396820083594E-5 0.0 0.0 0.03619732202677764 0.0 34-35 5.238396820083594E-5 0.0 0.0 0.06629191175815788 0.0 36-37 5.238396820083594E-5 0.0 0.0 0.11325413925020732 0.0 38-39 5.238396820083594E-5 0.0 0.0 0.182165249418407 0.0 40-41 5.238396820083594E-5 0.0 0.0 0.26257464060669017 0.0 42-43 5.238396820083594E-5 0.0 0.0 0.34198873639915744 0.0 44-45 5.238396820083594E-5 0.0 0.0 0.43714421463597597 0.0 46-47 5.238396820083594E-5 0.0 0.0 0.554824799199154 0.0 48-49 5.238396820083594E-5 0.0 0.0 0.6806510908175618 0.0 50-51 5.238396820083594E-5 2.619198410041797E-5 0.0 0.8219044610711159 0.0 52-53 5.238396820083594E-5 5.238396820083594E-5 0.0 0.9588623459322014 0.0 54-55 5.238396820083594E-5 5.238396820083594E-5 0.0 1.113604587997471 0.0 56-57 5.238396820083594E-5 5.238396820083594E-5 0.0 1.3029202490752918 0.0 58-59 5.238396820083594E-5 5.238396820083594E-5 0.0 1.5197636854426524 0.0 60-61 5.238396820083594E-5 5.238396820083594E-5 0.0 1.761437122737209 0.0 62-63 5.238396820083594E-5 5.238396820083594E-5 0.0 1.9976364353547784 0.0 64-65 5.238396820083594E-5 5.238396820083594E-5 0.0 2.2326309167037284 0.0 66-67 5.238396820083594E-5 5.238396820083594E-5 0.0 2.5101873722158574 0.0 68-69 5.238396820083594E-5 5.238396820083594E-5 0.0 2.8387396207715008 0.0 70-71 5.238396820083594E-5 5.238396820083594E-5 0.0 3.197517418979026 0.0 72-73 5.238396820083594E-5 5.238396820083594E-5 0.0 3.561350270117932 0.0 74-75 5.238396820083594E-5 5.238396820083594E-5 0.0 3.922432962926294 0.0 76-77 5.238396820083594E-5 5.238396820083594E-5 0.0 4.309550487930473 0.0 78-79 5.238396820083594E-5 5.238396820083594E-5 0.0 4.784961191337159 0.0 80-81 5.238396820083594E-5 5.238396820083594E-5 0.0 5.303457708589033 0.0 82-83 5.238396820083594E-5 5.238396820083594E-5 0.0 5.809172537599903 0.0 84-85 5.238396820083594E-5 5.238396820083594E-5 0.0 6.331362124609936 0.0 86-87 5.238396820083594E-5 5.238396820083594E-5 0.0 6.881131870877709 0.0 88-89 5.238396820083594E-5 5.238396820083594E-5 0.0 7.473489783292762 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCGA 45 9.01081E-4 26.35899 48-49 TCGCGAT 50 0.0016654256 23.72278 50-51 TACATGG 12460 0.0 22.732744 2 GTACATG 12565 0.0 22.698559 1 ACATGGG 12455 0.0 21.941904 3 AGTACTT 10225 0.0 21.532 12-13 GAGTACT 9945 0.0 21.255564 12-13 CATGGGG 3545 0.0 21.01256 4 GTACTTT 10645 0.0 19.83554 14-15 CATGGGA 8465 0.0 19.336937 4 TAAGGTG 670 0.0 19.11985 5 ATGGGAG 2885 0.0 19.076899 5 TACCTGG 1325 0.0 18.620144 2 ATGGGAT 3270 0.0 17.991589 5 TACTTTT 12230 0.0 17.846828 14-15 AGTACAT 9740 0.0 17.731195 2 AGAGTAC 20485 0.0 16.943691 10-11 ACTTTTT 12575 0.0 16.903513 16-17 GTAAGCG 225 7.2759576E-12 16.870415 94-95 ATGGGGA 1915 0.0 16.599752 5 >>END_MODULE