FastQCFastQC Report
Fri 27 May 2016
SRR939200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874625
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT165610.8834300193372008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122240.6520770820830832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93910.500953524038141No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT90670.48367006734680273No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT65510.3494565579782623No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC63590.3392145095685804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.3323331332933253No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44440.23706074548242984No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36750.19603920784156834No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35540.18958458358338334No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34480.18393011935720477No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34340.18318330332733213No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30190.1610455424418217No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29700.15843168633726745No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29000.15469760618790426No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26000.1386944055477762No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA24410.13021270920850836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23650.1261585650463426No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.11783690071347604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.11276922051076882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG127550.025.7620411
TACATGG129550.025.1923082
ACATGGG126400.024.6971993
GACCGTA1000.001685723723.672687
CATGGGA85600.022.4125184
GAGTACT90600.022.23648612-13
GTAAGCG1950.021.95073994-95
ATGGGAG31200.021.559725
AGTACTT92800.021.04605912-13
CATGGGG35700.020.5670914
GTACTTT98300.020.42296214-15
AACACGC1200.004855319419.7272327
GTCGGGA3707.2759576E-1219.2032932
GTGTAGC11300.018.489661
TACCTGG14650.018.4299182
ACTTTTT116250.017.82028816-17
TACGGGA1350.009554259517.5437492
AGTACAT94800.017.5381972
TACTTTT113500.017.08301214-15
ATGGGAT34000.016.858385
Produced by FastQC (version 0.11.5)