##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939200_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1874625 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26844728945789 33.0 31.0 34.0 31.0 34.0 2 32.347036340601456 34.0 31.0 34.0 31.0 34.0 3 32.409206107888245 34.0 31.0 34.0 30.0 34.0 4 35.83263052610522 37.0 35.0 37.0 35.0 37.0 5 35.88944615589784 37.0 35.0 37.0 35.0 37.0 6 35.992247516169904 37.0 37.0 37.0 35.0 37.0 7 35.93998693071948 37.0 37.0 37.0 35.0 37.0 8 35.94429872641195 37.0 37.0 37.0 35.0 37.0 9 37.630534106821365 39.0 38.0 39.0 35.0 39.0 10-11 37.54308541708342 39.0 38.0 39.0 35.0 39.0 12-13 37.46456064546243 39.0 38.0 39.0 35.0 39.0 14-15 38.74771354270854 40.0 38.0 41.0 35.0 41.0 16-17 38.689185837167436 40.0 38.0 41.0 35.0 41.0 18-19 38.63721304260852 40.0 38.0 41.0 34.5 41.0 20-21 38.64036914049477 40.0 38.5 41.0 34.5 41.0 22-23 38.679196372607855 40.0 38.5 41.0 35.0 41.0 24-25 38.623676201907045 40.5 38.0 41.0 34.0 41.0 26-27 38.5050351403614 40.0 38.0 41.0 34.0 41.0 28-29 38.37866479962659 40.0 38.0 41.0 34.0 41.0 30-31 38.16495405747816 40.0 38.0 41.0 34.0 41.0 32-33 37.94225671801027 40.0 38.0 41.0 33.5 41.0 34-35 37.61362779222512 40.0 37.0 41.0 33.0 41.0 36-37 37.348505701140226 40.0 37.0 41.0 32.5 41.0 38-39 37.16651116890044 40.0 37.0 41.0 32.0 41.0 40-41 37.00108928452357 40.0 36.5 41.0 31.0 41.0 42-43 36.77664306194572 40.0 36.0 41.0 31.0 41.0 44-45 36.460157098086285 40.0 36.0 41.0 30.0 41.0 46-47 36.215738614389544 40.0 36.0 41.0 30.0 41.0 48-49 36.03053117290125 40.0 36.0 41.0 28.5 41.0 50-51 34.88586223911449 38.5 34.5 40.0 26.0 40.5 52-53 35.0420276055211 38.5 35.0 40.0 26.5 40.5 54-55 35.58482123091285 39.0 35.0 41.0 27.0 41.0 56-57 35.49137800893512 39.0 35.0 41.0 27.0 41.0 58-59 35.31152283790091 39.0 35.0 41.0 26.5 41.0 60-61 35.00669627258785 39.0 35.0 41.0 26.0 41.0 62-63 34.66352417150097 38.0 35.0 40.0 25.5 41.0 64-65 34.31083443355338 38.0 34.5 40.0 24.0 41.0 66-67 33.91215309728612 37.0 34.0 40.0 22.5 41.0 68-69 33.48626952057078 36.5 34.0 39.0 22.0 41.0 70-71 33.042091885043675 36.0 33.5 39.0 20.5 41.0 72-73 32.59652570514103 35.5 33.0 38.5 19.5 40.0 74-75 32.16403200640128 35.0 33.0 37.5 18.0 40.0 76-77 31.731525771821033 35.0 32.0 37.0 14.0 39.0 78-79 31.298178035607123 35.0 32.0 36.5 9.5 39.0 80-81 30.816724678268987 35.0 31.0 36.0 6.5 38.5 82-83 30.43080669467227 35.0 31.0 36.0 2.0 37.0 84-85 30.04854224178169 35.0 31.0 35.0 2.0 37.0 86-87 29.672798826431954 34.0 30.5 35.0 2.0 36.5 88-89 29.241558178302327 34.0 30.0 35.0 2.0 36.0 90-91 28.81566073214643 34.0 29.0 35.0 2.0 36.0 92-93 28.408743882109754 34.0 29.0 35.0 2.0 35.0 94-95 27.938441021537642 34.0 29.0 35.0 2.0 35.0 96-97 27.372122691204908 34.0 27.0 35.0 2.0 35.0 98-99 26.71641554977662 34.0 25.5 35.0 2.0 35.0 100-101 23.83227525505101 30.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11760084194669673 1101 2 0.23247238031289896 1101 3 0.22139972697818422 1101 4 0.2698173524758545 1101 5 0.16250238215410207 1101 6 0.17259509700699027 1101 7 0.21447679898503935 1101 8 0.2534909556595579 1101 9 0.3208236611776911 1101 10-11 0.2368061892097657 1101 12-13 0.27350171499332987 1101 14-15 0.3953147109879467 1101 16-17 0.36115225513704985 1101 18-19 0.39144036271657257 1101 20-21 0.36409937612715026 1101 22-23 0.33313749902271894 1101 24-25 0.44964938361147944 1101 26-27 0.4156573282164473 1101 28-29 0.33574284826026 1101 30-31 0.4182538403902214 1101 32-33 0.4507106823210094 1101 34-35 0.33457131581851485 1101 36-37 0.31561751672257543 1101 38-39 0.21285381965628858 1101 40-41 0.2945228744544295 1101 42-43 0.42385097205102795 1101 44-45 0.26242878341818 1101 46-47 0.3547340932445948 1101 48-49 0.34678546512890307 1101 50-51 0.5707391693631934 1101 52-53 0.5081897554375345 1101 54-55 0.5003752620206043 1101 56-57 0.5537509613793077 1101 58-59 0.4629017802036657 1101 60-61 0.3973758012368478 1101 62-63 0.3327280499898677 1101 64-65 0.2103685290668338 1101 66-67 0.34886215276805643 1101 68-69 0.27457564541816026 1101 70-71 0.22187845242585524 1101 72-73 0.24804696273908178 1101 74-75 0.26351451982400675 1101 76-77 0.18836062841924317 1101 78-79 0.2420385856602536 1101 80-81 0.153152883172055 1101 82-83 -0.03689135889461781 1101 84-85 -0.039480817483873665 1101 86-87 -0.24828012840181302 1101 88-89 -0.14118211191405905 1101 90-91 -0.13326862611475931 1101 92-93 -0.03606924493674768 1101 94-95 -0.08747037353906961 1101 96-97 -0.2818466663055972 1101 98-99 -0.25566169456563514 1101 100-101 -0.660871896274525 1102 1 0.20863739413103843 1102 2 0.32316048572928935 1102 3 0.4300838254540551 1102 4 0.2924182491244878 1102 5 0.32669341937713625 1102 6 0.3409000159467439 1102 7 0.3215241447322299 1102 8 0.31970176082414525 1102 9 0.42953589584218577 1102 10-11 0.3631483307880643 1102 12-13 0.39821566703079725 1102 14-15 0.46686784043848917 1102 16-17 0.4681638880501495 1102 18-19 0.38659426924680673 1102 20-21 0.5125487485480846 1102 22-23 0.5047743661903183 1102 24-25 0.5264264522625979 1102 26-27 0.5266995786695574 1102 28-29 0.5742382843179072 1102 30-31 0.5729582861053331 1102 32-33 0.5738288034076362 1102 34-35 0.5338189391392731 1102 36-37 0.539431426417643 1102 38-39 0.5482444884649453 1102 40-41 0.5549970935370823 1102 42-43 0.5760361831493981 1102 44-45 0.527858602174561 1102 46-47 0.6759193410423379 1102 48-49 0.6653729495366036 1102 50-51 0.4399070761136272 1102 52-53 0.3362421274208387 1102 54-55 0.4262993464007607 1102 56-57 0.5026932498340386 1102 58-59 0.4197585062325615 1102 60-61 0.4114024607688478 1102 62-63 0.300500867432568 1102 64-65 0.283896716358349 1102 66-67 0.29385748247643306 1102 68-69 0.276625877788355 1102 70-71 0.21333578466530412 1102 72-73 0.15446670968702136 1102 74-75 0.24831269101121478 1102 76-77 0.24034786019317167 1102 78-79 0.20201909947520136 1102 80-81 0.3521801129255415 1102 82-83 -0.036994551227365946 1102 84-85 -0.05988409233102843 1102 86-87 0.03269444129800192 1102 88-89 -0.020841304930286242 1102 90-91 -0.09382567529547359 1102 92-93 -0.044916556983650935 1102 94-95 -0.03970948931297613 1102 96-97 -0.05079350863994492 1102 98-99 -0.06755864587345428 1102 100-101 -0.37901042497679427 1103 1 0.243217649381684 1103 2 0.14262283591789782 1103 3 0.21075380724135329 1103 4 0.20275310359342313 1103 5 0.22950104006184802 1103 6 0.24845862741597102 1103 7 0.18688318048232588 1103 8 0.16481195788965408 1103 9 0.21211923429144264 1103 10-11 0.22361483510931635 1103 12-13 0.1799412350692151 1103 14-15 0.28938780960650234 1103 16-17 0.37836231780165264 1103 18-19 0.2437320104627645 1103 20-21 0.29837217403631655 1103 22-23 0.37867529456051585 1103 24-25 0.3990535148337955 1103 26-27 0.4741897049306374 1103 28-29 0.29713751420037937 1103 30-31 0.4267024624752054 1103 32-33 0.46139948846435885 1103 34-35 0.4664268340376694 1103 36-37 0.33663437864852597 1103 38-39 0.47403455810976425 1103 40-41 0.35891555248347373 1103 42-43 0.4961322261504648 1103 44-45 0.49629844910602827 1103 46-47 0.521728714784679 1103 48-49 0.5289618745780444 1103 50-51 0.5029080106229458 1103 52-53 0.47421867601190826 1103 54-55 0.3908408570316553 1103 56-57 0.3610093595467987 1103 58-59 0.392192186766799 1103 60-61 0.4617482974275333 1103 62-63 0.28018211108029334 1103 64-65 0.3229175961613535 1103 66-67 0.23316724161859526 1103 68-69 0.19267522260864922 1103 70-71 0.20972394027133845 1103 72-73 0.22574350861744819 1103 74-75 0.15794836880331076 1103 76-77 0.23261939995074243 1103 78-79 0.19986358893980594 1103 80-81 0.1700804000995717 1103 82-83 0.1268464184795235 1103 84-85 -0.01230597212720852 1103 86-87 -0.01251841536737075 1103 88-89 0.09290450853619703 1103 90-91 0.04231084491925685 1103 92-93 -0.13947593925253443 1103 94-95 -0.07760145457924139 1103 96-97 -0.09662027653375205 1103 98-99 -0.1469169040026621 1103 100-101 -0.3268296758686695 1104 1 0.23080751456490844 1104 2 0.4027720919177753 1104 3 0.38237794152109217 1104 4 0.2539490261586437 1104 5 0.2790022534634815 1104 6 0.33822501593754595 1104 7 0.3308726626460867 1104 8 0.29853945676237714 1104 9 0.39871709931254884 1104 10-11 0.43130030316823564 1104 12-13 0.3708594685185673 1104 14-15 0.5362846426029151 1104 16-17 0.4730000452194716 1104 18-19 0.49462876239685727 1104 20-21 0.5321229783265409 1104 22-23 0.5987101006949089 1104 24-25 0.469544803167409 1104 26-27 0.4874813924961856 1104 28-29 0.5847934958397687 1104 30-31 0.5084652133250955 1104 32-33 0.48540533508431594 1104 34-35 0.4353204346740185 1104 36-37 0.5243861151554441 1104 38-39 0.24986810676487892 1104 40-41 0.5303686959283169 1104 42-43 0.5409292916944182 1104 44-45 0.4463779915092232 1104 46-47 0.6071278707232537 1104 48-49 0.6536813229489411 1104 50-51 0.6556167587673727 1104 52-53 0.6071684832355757 1104 54-55 0.6479692749484514 1104 56-57 0.7518766757253132 1104 58-59 0.7586863426800434 1104 60-61 0.6251712046263691 1104 62-63 0.5640534955740861 1104 64-65 0.6216677164882398 1104 66-67 0.5534634737405568 1104 68-69 0.41708232772114684 1104 70-71 0.44844234496484603 1104 72-73 0.44172013227041873 1104 74-75 0.31510287865491904 1104 76-77 0.3189203742185249 1104 78-79 0.3713129325883173 1104 80-81 0.3356653914107035 1104 82-83 0.41420601600509954 1104 84-85 0.3069275836208689 1104 86-87 0.3145237218407395 1104 88-89 0.3731078229718783 1104 90-91 0.5155919437190271 1104 92-93 0.34818738865975973 1104 94-95 0.14983155501144196 1104 96-97 0.3433340752463252 1104 98-99 0.24854816573626692 1104 100-101 0.024508359079323583 1105 1 0.1306772987738114 1105 2 0.1513389936445364 1105 3 0.2151893535860978 1105 4 0.18802788572501328 1105 5 0.20994440991150753 1105 6 0.24874220104064193 1105 7 0.22962177677156603 1105 8 0.20704026379664953 1105 9 0.14406725005785148 1105 10-11 0.17147460048946073 1105 12-13 0.19143804895252714 1105 14-15 0.1632586677614114 1105 16-17 0.16421092786446678 1105 18-19 0.249820314863058 1105 20-21 0.17722404910472278 1105 22-23 0.27370508045176933 1105 24-25 0.35887276685327407 1105 26-27 0.35034905198637745 1105 28-29 0.19585162507980414 1105 30-31 0.28891410932014594 1105 32-33 0.28333088781135274 1105 34-35 0.22104041344752545 1105 36-37 0.20718834931890484 1105 38-39 0.1664271803626889 1105 40-41 0.21411994581359295 1105 42-43 0.29626541336165246 1105 44-45 0.2649037927986839 1105 46-47 0.27089682774789736 1105 48-49 0.4220404038614589 1105 50-51 0.5217727637717431 1105 52-53 0.599588778681202 1105 54-55 0.6356800302759424 1105 56-57 0.6620113907583729 1105 58-59 0.6989388936881298 1105 60-61 0.5576839553538804 1105 62-63 0.48406515081897794 1105 64-65 0.43481581952639914 1105 66-67 0.4897800506179024 1105 68-69 0.3862021393602433 1105 70-71 0.429348514377871 1105 72-73 0.5446145870927168 1105 74-75 0.5878808086309775 1105 76-77 0.534218072911461 1105 78-79 0.45924568272236144 1105 80-81 0.34407662764644087 1105 82-83 0.3750532950333145 1105 84-85 0.18239432821919266 1105 86-87 0.152304312028118 1105 88-89 0.17065556358643974 1105 90-91 0.24388620656446847 1105 92-93 0.11504453703744488 1105 94-95 0.2263692158058639 1105 96-97 0.11193071199395277 1105 98-99 0.18546372145130263 1105 100-101 -0.05080770921806277 1106 1 0.19404982893122735 1106 2 0.1962041392154319 1106 3 0.2591995600003898 1106 4 0.21316071842782236 1106 5 0.24741968340614306 1106 6 0.19011874558006525 1106 7 0.13073029061774832 1106 8 0.18910239818463026 1106 9 0.34105474574681693 1106 10-11 0.3202270764434729 1106 12-13 0.2631931452223739 1106 14-15 0.30765154864209165 1106 16-17 0.36175406793574183 1106 18-19 0.40706205915036264 1106 20-21 0.512117723479939 1106 22-23 0.5593505595150177 1106 24-25 0.5509846647785608 1106 26-27 0.4283751335769068 1106 28-29 0.4832106162780221 1106 30-31 0.4859438894805379 1106 32-33 0.5103899103265164 1106 34-35 0.41910021690402033 1106 36-37 0.5151236910765462 1106 38-39 0.5950886522058525 1106 40-41 0.635305324900493 1106 42-43 0.5376899243483422 1106 44-45 0.5730403161430146 1106 46-47 0.6372280155847747 1106 48-49 0.5962881036522845 1106 50-51 0.6375050317125854 1106 52-53 0.5487919146033065 1106 54-55 0.45096130790125244 1106 56-57 0.4304834281695591 1106 58-59 0.5592528030328694 1106 60-61 0.6388153028146348 1106 62-63 0.6346203327148814 1106 64-65 0.6214413072843783 1106 66-67 0.5317394601681116 1106 68-69 0.47764561228959224 1106 70-71 0.45568777590600007 1106 72-73 0.5128444137660253 1106 74-75 0.5756652212534235 1106 76-77 0.4986393240296856 1106 78-79 0.539553474032644 1106 80-81 0.4747538134562994 1106 82-83 0.7497607723813928 1106 84-85 0.6961460311602075 1106 86-87 0.658753684962619 1106 88-89 0.5733206745369266 1106 90-91 0.6802365149107636 1106 92-93 0.6921430030361009 1106 94-95 0.8013250483540695 1106 96-97 0.6399577185572944 1106 98-99 0.8119487983363669 1106 100-101 0.697570945580761 1107 1 0.13199271612285202 1107 2 0.1591808541708346 1107 3 0.2035354135822871 1107 4 0.19874656399366586 1107 5 0.24786986521033327 1107 6 0.18922351224865253 1107 7 0.25236402731911056 1107 8 0.2737128498413881 1107 9 0.2812113474513538 1107 10-11 0.28232843789072604 1107 12-13 0.18068418326978986 1107 14-15 0.24481816079956076 1107 16-17 0.23482845053897705 1107 18-19 0.23553060734610654 1107 20-21 0.41066393124332734 1107 22-23 0.37984248894805006 1107 24-25 0.4101832513872594 1107 26-27 0.29034727617295886 1107 28-29 0.3754481865788435 1107 30-31 0.4508896688657984 1107 32-33 0.42650058322646345 1107 34-35 0.4526150337852002 1107 36-37 0.479379768851544 1107 38-39 0.5719443695788939 1107 40-41 0.5833635150670275 1107 42-43 0.5177636079513377 1107 44-45 0.48091176422781956 1107 46-47 0.6721902118563392 1107 48-49 0.6204645338773105 1107 50-51 0.5923153076573655 1107 52-53 0.5677421958066233 1107 54-55 0.5546236843738015 1107 56-57 0.47365086117135036 1107 58-59 0.671067830896682 1107 60-61 0.720801297158502 1107 62-63 0.6763669996380628 1107 64-65 0.6547101915132671 1107 66-67 0.547952738607485 1107 68-69 0.5296332739734808 1107 70-71 0.5126729686440967 1107 72-73 0.4919321222636839 1107 74-75 0.3563548587352301 1107 76-77 0.4381232181664281 1107 78-79 0.5248610020728108 1107 80-81 0.5337680307363577 1107 82-83 0.6047938947320866 1107 84-85 0.5014747857213457 1107 86-87 0.5619719718348826 1107 88-89 0.6266620880564879 1107 90-91 0.7618903238207686 1107 92-93 0.6656874472667873 1107 94-95 0.7748508953307365 1107 96-97 0.9743850834546564 1107 98-99 0.8386618298811577 1107 100-101 0.6358056172750999 1108 1 0.07861295192621043 1108 2 0.08859965049159513 1108 3 0.0312020425002828 1108 4 0.1796526578871891 1108 5 0.15994000223800953 1108 6 0.22144703470281968 1108 7 0.024933956729093154 1108 8 0.2401781902714717 1108 9 0.1758699107369992 1108 10-11 0.13504193807317222 1108 12-13 0.25363097022888326 1108 14-15 0.2360818604208177 1108 16-17 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-1.965494748004236 2316 100-101 -1.7673420824827701 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4912.0 3 1128.0 4 1092.0 5 1208.0 6 1460.0 7 1769.0 8 2183.0 9 2718.0 10 3516.0 11 4679.0 12 6804.0 13 11462.0 14 10166.0 15 10499.0 16 14053.0 17 21346.0 18 17623.0 19 10672.0 20 10063.0 21 10061.0 22 10776.0 23 11613.0 24 12964.0 25 14621.0 26 17100.0 27 19418.0 28 23119.0 29 27648.0 30 33335.0 31 40752.0 32 52430.0 33 68296.0 34 95976.0 35 147710.0 36 234569.0 37 396380.0 38 433118.0 39 87330.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.31418521981559 19.10923464311228 14.085184364051928 21.4913957730202 2 14.894920363141722 21.565051320818803 40.42330930226224 23.11671901377723 3 20.849255267389687 25.911428917810507 29.753895633291087 23.48542018150871 4 12.243272649773978 16.54944980987314 38.59636753266662 32.610910007686265 5 12.679693805441145 38.98607104265005 33.857242742675105 14.476992409233697 6 29.827850544719997 36.58181262998757 18.064162079288682 15.526174746003749 7 27.390249579833725 34.26426589958293 20.957060822137798 17.388423698445546 8 25.607897340130485 33.82651669365348 20.174125010610542 20.39146095560549 9 26.45982615232012 17.96641350805655 21.682485075814117 33.89127526380921 10-11 24.85467913564215 26.84348695335345 26.991203473233387 21.310630437771014 12-13 26.948470901400196 24.763853660541873 27.41441046566195 20.873264972395983 14-15 23.188078916560208 25.788041868035716 27.785512532170557 23.238366683233515 16-17 20.865769089094005 29.23614262301208 28.209177926424978 21.68891036146894 18-19 20.59498442878217 28.431954605615463 30.64836383419816 20.324697131404207 20-21 22.248893171901717 26.429119622857584 30.198167944229475 21.12381926101122 22-23 21.2750649207109 26.74511703081087 30.319887509033638 21.659930539444588 24-25 21.76753865304298 26.780485746982436 30.540776804275932 20.91119879569865 26-27 21.505788918162125 26.318116679790226 31.518864415609105 20.657229986438544 28-29 20.474330926293092 27.949683371771577 30.822592608216688 20.753393093718646 30-31 20.976024791592472 27.030161359992267 30.53746263134093 21.456351217074335 32-33 20.962951671415496 27.123791024542715 30.674372643550697 21.2388846604911 34-35 21.855011260131107 26.796194972214305 30.519946422382706 20.828847345271882 36-37 21.57808977873184 26.421064005896692 31.121474002070492 20.87937221330097 38-39 21.18621735352835 27.237859787085345 30.940288620504862 20.63563423888144 40-41 21.185983249581238 27.446378559463984 30.546385259631492 20.821252931323283 42-43 20.63601381372912 27.704531818920263 29.782623032211124 21.876831335139492 44-45 21.3155374529606 27.124883239334967 29.72806963922429 21.83150966848014 46-47 21.876384058647087 27.74548977847555 29.27885249540747 21.09927366746989 48-49 21.707483565905026 27.567775392747922 29.320843832260962 21.403897209086086 50-51 20.78284519568851 28.0408305748851 29.961922421693227 21.214401807733164 52-53 21.494561356001277 28.579732370991394 28.65153266001571 21.274173612991614 54-55 20.72213631255201 27.75843320779321 29.270785969818895 22.248644509835884 56-57 21.647694318265877 27.749605740377227 28.723301038529698 21.879398902827198 58-59 22.33553089993032 27.4542798949456 28.710725196977005 21.499464008147076 60-61 21.534316790067503 27.67960888871746 29.858556443227315 20.92751787798772 62-63 21.250541808881724 27.69772572957565 29.661345658921356 21.39038680262127 64-65 20.86893710329095 28.490040865070725 29.14113102482283 21.499891006815492 66-67 21.15736975460743 27.842629977242066 28.527781330771962 22.472218937378543 68-69 20.93320449413854 27.903099194111373 28.96087327442239 22.202823037327697 70-71 21.575968185425186 27.819650901067 28.664574694068452 21.93980621943936 72-73 21.479993972101123 27.19856081232909 29.521554296942664 21.799890918627117 74-75 20.643586273398803 28.766380537150493 28.700280516154013 21.88975267329669 76-77 21.04793853803987 27.634316672149172 28.735964989181102 22.58177980062986 78-79 21.166894263357293 27.74716799584027 28.98329275009615 22.10264499070628 80-81 21.445961720679705 27.75370000817533 28.745595707012157 22.054742564132805 82-83 21.203845735232537 27.575194450148544 29.227705721474788 21.993254093144127 84-85 20.874437700830704 27.83546082864689 29.055557208621067 22.234544261901334 86-87 20.82308195863493 27.877296607275266 28.954930766026322 22.34469066806348 88-89 20.870322168475795 27.689288154877644 28.501043010755573 22.939346665890987 90-91 20.987063090727293 27.154317026808823 28.832197719369322 23.026422163094562 92-93 21.45892824150485 27.598554870901854 28.89341113697594 22.049105750617354 94-95 21.020378120221174 27.238309804166576 29.043775270872107 22.69753680474014 96-97 20.287026735837102 27.09954766783068 29.69250046724098 22.92092512909124 98-99 20.829753596094093 27.375551172276797 28.57735074774946 23.21734448387965 100-101 20.887406196852034 26.367424169309988 29.38330663200856 23.361863001829416 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3343.0 1 2017.5 2 974.5 3 1278.0 4 1073.0 5 1024.5 6 1773.5 7 3340.0 8 5036.0 9 5605.5 10 4669.0 11 3797.5 12 3840.5 13 3993.5 14 4115.0 15 4249.5 16 4465.0 17 4623.0 18 4632.0 19 4713.0 20 4988.5 21 5538.5 22 6482.0 23 7698.0 24 8888.0 25 10565.0 26 13290.0 27 16445.0 28 18823.5 29 21139.0 30 24378.5 31 27922.0 32 31918.5 33 36110.0 34 40193.5 35 42933.0 36 46824.0 37 51547.5 38 52754.0 39 52948.5 40 53701.5 41 55395.5 42 56958.0 43 58617.0 44 61575.0 45 63915.5 46 66485.5 47 77684.5 48 105844.0 49 104193.5 50 75238.5 51 62382.0 52 55649.0 53 52474.0 54 49878.5 55 45446.0 56 40340.0 57 35476.5 58 31425.5 59 27390.0 60 23266.5 61 19454.5 62 15883.0 63 11690.0 64 8223.5 65 6455.5 66 4926.5 67 3862.0 68 2942.5 69 1990.5 70 1507.0 71 1300.0 72 1068.5 73 920.5 74 764.0 75 616.5 76 455.0 77 285.0 78 195.5 79 135.0 80 86.5 81 59.5 82 49.0 83 35.5 84 25.0 85 21.0 86 14.5 87 7.5 88 6.5 89 7.0 90 5.0 91 4.0 92 4.0 93 2.5 94 2.5 95 2.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38871774354870975 2 0.11671667666866706 3 0.11778355671134227 4 0.13090618123624725 5 0.1323998132959925 6 0.07302793892111756 7 0.08305661132226445 8 0.07937587517503501 9 0.07953590718143629 10-11 0.07694872307794892 12-13 0.0815096352603854 14-15 0.07500166700006668 16-17 0.07654864306194573 18-19 0.08631059545242381 20-21 0.08495032339801294 22-23 0.09407214776288592 24-25 0.10690138027605522 26-27 0.10874174834966993 28-29 0.10263386010535441 30-31 0.09239181169567247 32-33 0.09103153964126158 34-35 0.33646729345869175 36-37 0.5255184370207375 38-39 0.507381476295259 40-41 0.48009601920384076 42-43 0.5080482763219311 44-45 0.5173834766953391 46-47 0.5141828365673135 48-49 0.5202107088084283 50-51 0.5199973327998932 52-53 0.5190371407614855 54-55 0.5130092685203708 56-57 0.48559045142361806 58-59 0.4760952190438088 60-61 0.42376475295059013 62-63 0.49852637194105487 64-65 0.5243448689737947 66-67 0.5334666933386677 68-69 0.5486697339467893 70-71 0.5439221177568847 72-73 0.5314129492565179 74-75 0.5348536373941455 76-77 0.5121824364872974 78-79 0.4863639394545575 80-81 0.49383209975328396 82-83 0.5064479562579183 84-85 0.5016203240648129 86-87 0.5029272521170901 88-89 0.5116489964659598 90-91 0.5292525171701007 92-93 0.5343201973728079 94-95 0.5309595252383811 96-97 0.5310662132426486 98-99 0.5314662932586517 100-101 0.509381876375275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1874625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.62245064426154 #Duplication Level Percentage of deduplicated Percentage of total 1 74.89964194792962 39.41402711677788 2 14.169071456299125 14.912225267682317 3 4.901800593825305 7.738342794497521 4 2.217954862968576 4.668568812310551 5 1.130701350160357 2.9750137996106636 6 0.6298222306041761 1.9885673546776184 7 0.41848404020577695 1.5415159025797764 8 0.2741094688157226 1.1539449595104088 9 0.20486568126098087 0.9702480780773084 >10 1.020099805743732 9.802601083074787 >50 0.08332020122581746 3.0426730291270276 >100 0.04434508797921211 4.235170672114935 >500 0.002029218590045535 0.6617308742614496 >1k 0.0030438278850683028 3.351100397209931 >5k 5.073046475113838E-4 2.0074033734453756 >10k+ 2.029218590045535E-4 1.5368664850424258 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16561 0.8834300193372008 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12224 0.6520770820830832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9391 0.500953524038141 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9067 0.48367006734680273 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6551 0.3494565579782623 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6359 0.3392145095685804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6230 0.3323331332933253 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4444 0.23706074548242984 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3675 0.19603920784156834 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3554 0.18958458358338334 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3448 0.18393011935720477 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3434 0.18318330332733213 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3019 0.1610455424418217 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2970 0.15843168633726745 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2900 0.15469760618790426 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2600 0.1386944055477762 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2441 0.13021270920850836 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2365 0.1261585650463426 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2209 0.11783690071347604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.11276922051076882 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.3344002133760084E-5 0.0 10-11 0.0 0.0 0.0 5.3344002133760084E-5 0.0 12-13 0.0 0.0 0.0 1.6003200640128025E-4 0.0 14-15 0.0 0.0 0.0 1.6003200640128025E-4 0.0 16-17 0.0 2.6672001066880042E-5 0.0 3.467360138694406E-4 0.0 18-19 0.0 5.3344002133760084E-5 0.0 5.86784023471361E-4 0.0 20-21 0.0 5.3344002133760084E-5 0.0 7.734880309395213E-4 0.0 22-23 0.0 5.3344002133760084E-5 0.0 0.001200240048009602 0.0 24-25 0.0 5.3344002133760084E-5 0.0 0.0019470560778822431 0.0 26-27 0.0 5.3344002133760084E-5 0.0 0.0030406081216243245 0.0 28-29 0.0 5.3344002133760084E-5 0.0 0.00632126425285057 0.0 30-31 0.0 5.3344002133760084E-5 0.0 0.017230112689204508 0.0 32-33 0.0 5.3344002133760084E-5 0.0 0.035900513436020534 0.0 34-35 0.0 5.3344002133760084E-5 0.0 0.06553310662132426 0.0 36-37 0.0 5.3344002133760084E-5 0.0 0.10828832433153297 0.0 38-39 0.0 5.3344002133760084E-5 0.0 0.17632859905314396 0.0 40-41 0.0 5.3344002133760084E-5 0.0 0.2538107621524305 0.0 42-43 0.0 5.3344002133760084E-5 0.0 0.3325198373007935 0.0 44-45 0.0 5.3344002133760084E-5 0.0 0.4293658731746349 0.0 46-47 0.0 5.3344002133760084E-5 0.0 0.546509301860372 0.0 48-49 0.0 5.3344002133760084E-5 0.0 0.6714676268587051 0.0 50-51 0.0 5.3344002133760084E-5 0.0 0.8075215043008601 0.0 52-53 0.0 5.3344002133760084E-5 0.0 0.9447489497899579 0.0 54-55 0.0 5.3344002133760084E-5 0.0 1.0960325398413016 0.0 56-57 0.0 5.3344002133760084E-5 0.0 1.2848703073948124 0.0 58-59 0.0 5.3344002133760084E-5 0.0 1.4942721877708873 0.0 60-61 0.0 5.3344002133760084E-5 0.0 1.7270120690804829 0.0 62-63 0.0 5.3344002133760084E-5 0.0 1.955724478228979 0.0 64-65 0.0 5.3344002133760084E-5 0.0 2.190331399613256 0.0 66-67 0.0 5.3344002133760084E-5 0.0 2.4657198106287925 0.0 68-69 0.0 5.3344002133760084E-5 0.0 2.7867973594718944 0.0 70-71 0.0 5.3344002133760084E-5 0.0 3.1448956457958257 0.0 72-73 0.0 5.3344002133760084E-5 0.0 3.500886844035474 0.0 74-75 0.0 5.3344002133760084E-5 0.0 3.862159098486364 0.0 76-77 0.0 5.3344002133760084E-5 0.0 4.247382809895312 0.0 78-79 0.0 5.3344002133760084E-5 0.0 4.724464892978595 0.0 80-81 0.0 5.3344002133760084E-5 0.0 5.249263185970527 0.0 82-83 0.0 5.3344002133760084E-5 0.0 5.7541374941655 0.0 84-85 0.0 5.3344002133760084E-5 0.0 6.269920650796826 0.0 86-87 0.0 5.3344002133760084E-5 0.0 6.819470560778822 0.0 88-89 0.0 5.3344002133760084E-5 0.0 7.4028672401146896 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12755 0.0 25.762041 1 TACATGG 12955 0.0 25.192308 2 ACATGGG 12640 0.0 24.697199 3 GACCGTA 100 0.0016857237 23.67268 7 CATGGGA 8560 0.0 22.412518 4 GAGTACT 9060 0.0 22.236486 12-13 GTAAGCG 195 0.0 21.950739 94-95 ATGGGAG 3120 0.0 21.55972 5 AGTACTT 9280 0.0 21.046059 12-13 CATGGGG 3570 0.0 20.567091 4 GTACTTT 9830 0.0 20.422962 14-15 AACACGC 120 0.0048553194 19.727232 7 GTCGGGA 370 7.2759576E-12 19.203293 2 GTGTAGC 1130 0.0 18.48966 1 TACCTGG 1465 0.0 18.429918 2 ACTTTTT 11625 0.0 17.820288 16-17 TACGGGA 135 0.0095542595 17.543749 2 AGTACAT 9480 0.0 17.538197 2 TACTTTT 11350 0.0 17.083012 14-15 ATGGGAT 3400 0.0 16.85838 5 >>END_MODULE