FastQCFastQC Report
Fri 27 May 2016
SRR939200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874625
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT179090.9553377342135093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121850.6499966659998666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98800.5270387410815497No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT83820.44712942588517707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65720.35057678202307124No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61770.32950590118023604No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC59640.31814362872574514No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47080.2511435620457425No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39180.20900180036007204No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37440.19971994398879775No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37430.199666599986664No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG37180.19833299993332No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32910.17555511102220445No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30230.16125891845035673No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28440.15171034206841366No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26390.14077482163099286No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26170.13960125358405015No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24600.1312262452490498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.12098419683936787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22080.11778355671134227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG4800.032.62484794-95
GTACATG124500.024.5726661
TACATGG124750.023.9649092
ACATGGG125550.022.9050753
CGCGGAA1053.2971002E-822.71696582-83
GAGTACT103400.022.03026212-13
GTGTAGC9900.021.6602731
AGTACTT105000.020.76802412-13
GTACTTT109850.020.71512214-15
TACCTGG13950.020.4104612
CATGGGA83250.020.292784
ATGGGCG2351.1443808E-720.1933295
ATTCGCG1201.4137368E-719.79375380-81
GTATAGG5100.019.621661
CTCCGAC4250.019.54001694-95
CATGGGG36950.019.521134
CCCGTAG1300.00742620418.3279231
ATCACGC1300.007606946418.2516633
ATGGGAG28350.018.0778375
ACTTTTT126750.017.98982816-17
Produced by FastQC (version 0.11.5)