##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939200_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1874625 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50348949789958 34.0 31.0 34.0 31.0 34.0 2 32.69992131759685 34.0 31.0 34.0 31.0 34.0 3 32.80650316730013 34.0 31.0 34.0 31.0 34.0 4 36.24536480629459 37.0 35.0 37.0 35.0 37.0 5 36.22155364406215 37.0 37.0 37.0 35.0 37.0 6 36.28158805094352 37.0 37.0 37.0 35.0 37.0 7 36.23837514169501 37.0 37.0 37.0 35.0 37.0 8 36.22440701473628 37.0 37.0 37.0 35.0 37.0 9 37.95755897846236 39.0 38.0 39.0 35.0 39.0 10-11 37.87557351470294 39.0 38.0 39.0 35.0 39.0 12-13 37.79988397679536 39.0 38.0 39.0 35.0 39.0 14-15 39.1604075481763 40.5 38.5 41.0 36.0 41.0 16-17 39.28091138227646 41.0 39.0 41.0 36.0 41.0 18-19 39.35393532039741 41.0 39.0 41.0 36.0 41.0 20-21 39.33426605321064 41.0 39.0 41.0 36.0 41.0 22-23 39.260087750883514 41.0 39.0 41.0 36.0 41.0 24-25 39.178282856571315 41.0 39.0 41.0 35.5 41.0 26-27 39.014498366339936 40.5 38.5 41.0 35.0 41.0 28-29 38.872939921317595 40.0 38.0 41.0 35.0 41.0 30-31 38.6742649863306 40.0 38.0 41.0 35.0 41.0 32-33 38.53209361872375 40.0 38.0 41.0 35.0 41.0 34-35 38.392667600186705 40.0 38.0 41.0 34.0 41.0 36-37 38.22060198706408 40.0 38.0 41.0 34.0 41.0 38-39 38.0809575248383 40.0 38.0 41.0 34.0 41.0 40-41 37.90817763552711 40.0 37.5 41.0 33.0 41.0 42-43 37.68891698339668 40.0 37.0 41.0 33.0 41.0 44-45 37.38740921517637 40.0 37.0 41.0 33.0 41.0 46-47 36.999501500300056 40.0 37.0 41.0 31.5 41.0 48-49 36.583712742548514 40.0 36.0 41.0 30.5 41.0 50-51 36.30322224444889 40.0 36.0 41.0 30.0 41.0 52-53 36.06188117623525 39.0 35.5 41.0 29.5 41.0 54-55 35.84552297126092 39.0 35.0 41.0 29.0 41.0 56-57 35.62200946856038 39.0 35.0 40.0 28.0 41.0 58-59 35.310564246182565 38.5 35.0 40.0 28.0 41.0 60-61 34.915837567513506 38.0 34.5 40.0 26.5 41.0 62-63 34.55211335600453 38.0 34.0 40.0 26.0 41.0 64-65 34.117788624391544 37.0 34.0 40.0 26.0 41.0 66-67 33.69675161699006 36.5 33.5 39.0 25.0 41.0 68-69 33.201281856371274 36.0 33.0 39.0 23.0 40.0 70-71 32.68276268587051 35.5 32.5 38.5 22.0 40.0 72-73 32.10611855704474 35.0 32.0 37.5 20.5 39.5 74-75 31.57307621524305 35.0 31.0 37.0 18.5 39.0 76-77 29.58008401680336 33.0 28.5 35.0 13.0 37.5 78-79 30.55688817763553 34.0 30.5 36.0 13.5 38.0 80-81 30.551156898046276 34.0 31.0 36.0 12.0 37.0 82-83 30.156543575381743 34.0 30.5 35.0 7.0 37.0 84-85 29.684428885777155 34.0 30.0 35.0 2.0 36.5 86-87 29.0218899779956 34.0 29.0 35.0 2.0 36.0 88-89 28.340792425151697 33.5 28.5 35.0 2.0 35.5 90-91 27.772112022404478 33.0 27.0 35.0 2.0 35.0 92-93 27.354112422484498 33.0 26.0 35.0 2.0 35.0 94-95 26.84136667333467 33.0 25.5 34.5 2.0 35.0 96-97 26.048640928185637 32.0 24.5 34.0 2.0 35.0 98-99 24.90844968993799 32.0 12.0 34.0 2.0 35.0 100-101 19.143300926852035 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12999863080064955 1101 2 0.2179833123824082 1101 3 0.17874212893514851 1101 4 0.14603024982521617 1101 5 0.1430286263060765 1101 6 0.17154374392110583 1101 7 0.17033426773762983 1101 8 0.19006421453477174 1101 9 0.17964203884416463 1101 10-11 0.30341470914746793 1101 12-13 0.26378774697656837 1101 14-15 0.38290856488166725 1101 16-17 0.289149458820674 1101 18-19 0.2881363064334792 1101 20-21 0.27224384211285013 1101 22-23 0.30167169429778795 1101 24-25 0.2644465057574763 1101 26-27 0.27016103080816123 1101 28-29 0.17323366808862062 1101 30-31 0.29296352708513496 1101 32-33 0.355962656357633 1101 34-35 0.3301423366342462 1101 36-37 0.400694405556024 1101 38-39 0.27237939126454336 1101 40-41 0.22998167566299088 1101 42-43 0.1982905041190719 1101 44-45 0.3111946022507439 1101 46-47 0.5159496475441827 1101 48-49 0.696947092282926 1101 50-51 0.5854608902038052 1101 52-53 0.5185525428904398 1101 54-55 0.5166859124991561 1101 56-57 0.5117689913471324 1101 58-59 0.6181703901320361 1101 60-61 0.7554401058038636 1101 62-63 0.6046265577875047 1101 64-65 0.6100447208606994 1101 66-67 0.6047373620244016 1101 68-69 0.6532993240035978 1101 70-71 0.8857667812234453 1101 72-73 0.6092535209800118 1101 74-75 0.5657720661717853 1101 76-77 0.7472267790802221 1101 78-79 0.834276933352033 1101 80-81 0.8377778839220049 1101 82-83 0.6723750589238584 1101 84-85 0.577640153806172 1101 86-87 0.6315559593565041 1101 88-89 0.487219966215374 1101 90-91 0.6637594474846047 1101 92-93 0.6509758860941695 1101 94-95 0.4552956859317483 1101 96-97 0.3688730079324962 1101 98-99 0.08760057271111421 1101 100-101 -1.110935666528853 1102 1 0.16122822170520124 1102 2 0.17000420498226987 1102 3 0.13023502405425091 1102 4 0.10666799817246186 1102 5 0.15468048102547982 1102 6 0.155616214534966 1102 7 0.1919146849503477 1102 8 0.19423413185857186 1102 9 0.24243151504146 1102 10-11 0.24594388021904479 1102 12-13 0.29180890475903 1102 14-15 0.3558615190433514 1102 16-17 0.26744749958697867 1102 18-19 0.2671448550204971 1102 20-21 0.3190337713304032 1102 22-23 0.2939257581618051 1102 24-25 0.30703573752903424 1102 26-27 0.3171648424132911 1102 28-29 0.26299225327635867 1102 30-31 0.2519172475929494 1102 32-33 0.19897456234566846 1102 34-35 0.09905366344298017 1102 36-37 0.2206050406012423 1102 38-39 0.2504021867073547 1102 40-41 0.23532182955570846 1102 42-43 0.33747269205697705 1102 44-45 0.329839858580101 1102 46-47 0.33852197392554473 1102 48-49 0.1793197503792996 1102 50-51 0.04678981014115635 1102 52-53 0.2787260638562401 1102 54-55 0.24179771734686284 1102 56-57 0.21925207313189077 1102 58-59 0.22257952425168526 1102 60-61 0.04609522124313514 1102 62-63 0.019843500429530536 1102 64-65 0.1759345051354515 1102 66-67 0.19008551085655512 1102 68-69 0.3231434463616907 1102 70-71 0.2777230276743623 1102 72-73 0.2634988298723542 1102 74-75 0.17638339491414357 1102 76-77 0.2609103191731066 1102 78-79 0.42671832188280945 1102 80-81 0.45388662141215264 1102 82-83 0.2634177276709444 1102 84-85 0.31474851128019665 1102 86-87 0.5444683390032665 1102 88-89 0.4814267920626243 1102 90-91 0.5122811926543953 1102 92-93 0.6053997986186381 1102 94-95 0.5356554818832144 1102 96-97 0.44484683377935497 1102 98-99 0.4098159680600766 1102 100-101 -0.2314960778459927 1103 1 0.12619435426910286 1103 2 0.12434967329421909 1103 3 0.17553826209491774 1103 4 0.1390927428877049 1103 5 0.16052628016736747 1103 6 0.17141760743133005 1103 7 0.08622645635709603 1103 8 0.11323195588433066 1103 9 0.17475803396015976 1103 10-11 0.16393045602684708 1103 12-13 0.13054977282950375 1103 14-15 0.05177252920017139 1103 16-17 0.17176431870826292 1103 18-19 0.22323655971555212 1103 20-21 0.2763584144092377 1103 22-23 0.18418059953396693 1103 24-25 0.26316495902138826 1103 26-27 0.2074964226890046 1103 28-29 0.1509756231033066 1103 30-31 0.23352549037437598 1103 32-33 0.14826378956785646 1103 34-35 0.21522442626179128 1103 36-37 0.28868267990793584 1103 38-39 0.2635296551420794 1103 40-41 0.28443984375752507 1103 42-43 0.1481083630278448 1103 44-45 0.1876716605459805 1103 46-47 0.2383913472586059 1103 48-49 0.22847869199634374 1103 50-51 0.2847742031534821 1103 52-53 0.2728512744618996 1103 54-55 0.13335712007945233 1103 56-57 0.1438313734095118 1103 58-59 0.18924073392965113 1103 60-61 0.27634092215922124 1103 62-63 0.14585048537507106 1103 64-65 0.2686487431010889 1103 66-67 0.16145337328587317 1103 68-69 0.10723427793855933 1103 70-71 0.2197459333844094 1103 72-73 0.0714554604546791 1103 74-75 0.09734402956193122 1103 76-77 0.10161229255664495 1103 78-79 0.1050339541090537 1103 80-81 0.11834832358335134 1103 82-83 0.07887260178504008 1103 84-85 -0.13240737442317396 1103 86-87 -0.07460656498783891 1103 88-89 -0.038037604496739874 1103 90-91 0.17022520395036267 1103 92-93 0.013918499036780219 1103 94-95 -0.12691503264261073 1103 96-97 -0.22604217789177738 1103 98-99 -0.4007873020403956 1103 100-101 -0.5191032565146259 1104 1 0.10743349318458684 1104 2 0.10305561465576574 1104 3 0.17314945680154636 1104 4 0.06942221056157649 1104 5 0.05429917394025807 1104 6 0.08204893092572974 1104 7 0.05729227389593916 1104 8 0.0502617310883835 1104 9 0.1781373194971252 1104 10-11 0.19537883706029646 1104 12-13 0.11703389728042879 1104 14-15 0.061795125322355204 1104 16-17 0.09330739066626847 1104 18-19 0.1533101744364629 1104 20-21 0.23616543541958634 1104 22-23 0.18552699403388573 1104 24-25 0.12415827644623789 1104 26-27 0.16682340276287277 1104 28-29 0.1698568110716323 1104 30-31 0.16084931915048628 1104 32-33 0.08938087902519243 1104 34-35 0.07059784491321608 1104 36-37 0.09490193322262286 1104 38-39 0.17207362385202174 1104 40-41 0.20912154748487666 1104 42-43 0.18801635729268185 1104 44-45 0.2922927195238856 1104 46-47 0.5034968042727783 1104 48-49 0.4019305283112544 1104 50-51 0.3664938896449499 1104 52-53 0.34778705790390774 1104 54-55 0.30297242869891505 1104 56-57 0.3441731017595444 1104 58-59 0.39920734638589295 1104 60-61 0.3305652824001868 1104 62-63 0.32705897260014893 1104 64-65 0.4019506647847493 1104 66-67 0.37206848431592476 1104 68-69 0.3923963265860806 1104 70-71 0.4099662709076526 1104 72-73 0.37716554106102507 1104 74-75 0.12065324685455181 1104 76-77 0.14594198315189288 1104 78-79 0.2212177933219408 1104 80-81 0.41166853495335687 1104 82-83 0.3724397999165525 1104 84-85 0.12295413819974854 1104 86-87 0.23624210685668245 1104 88-89 0.6069600902934837 1104 90-91 0.6862286794974075 1104 92-93 0.34198404805668403 1104 94-95 0.6635819300535246 1104 96-97 0.3740191254890277 1104 98-99 0.18082345695625435 1104 100-101 -0.11311021774150376 1105 1 0.08447562803349484 1105 2 0.10175759715150434 1105 3 0.08179621317243146 1105 4 0.04136715971392846 1105 5 8.316868290023649E-4 1105 6 0.043264361117437033 1105 7 0.05802616098566915 1105 8 0.021769867332601223 1105 9 0.17073185705854854 1105 10-11 0.10117682109626713 1105 12-13 0.13991516490735023 1105 14-15 0.06927804786983671 1105 16-17 0.04111825412739023 1105 18-19 0.10297611262156892 1105 20-21 0.08013541609077635 1105 22-23 0.1376588894499733 1105 24-25 0.056495577439314104 1105 26-27 0.17357312828841032 1105 28-29 0.08705109206584893 1105 30-31 0.10840520256491004 1105 32-33 0.08489112549670352 1105 34-35 0.0825677607180424 1105 36-37 0.19006124572188554 1105 38-39 0.08240535323780307 1105 40-41 0.05255586303768922 1105 42-43 0.10020005609741389 1105 44-45 0.042161434127720554 1105 46-47 0.05814655001730529 1105 48-49 -0.05474028796303543 1105 50-51 -0.033157315592937664 1105 52-53 -0.1337399999989941 1105 54-55 -0.21942872426634352 1105 56-57 -0.2261988033727036 1105 58-59 -0.25901312061058235 1105 60-61 -0.31387884384633935 1105 62-63 -0.30570213352330455 1105 64-65 -0.30264408255727915 1105 66-67 -0.3463854119103047 1105 68-69 -0.3183439919354072 1105 70-71 -0.43320081589528314 1105 72-73 -0.5865458643103239 1105 74-75 -0.6080108923238434 1105 76-77 -0.6665360640666833 1105 78-79 -0.5710467883279193 1105 80-81 -0.3437992171439319 1105 82-83 -0.2046757926481071 1105 84-85 -0.4735006040495797 1105 86-87 -0.418597718059317 1105 88-89 -0.5497991581674917 1105 90-91 -0.3260705711579881 1105 92-93 -0.36221028745289274 1105 94-95 -0.745616535511747 1105 96-97 -0.9865340466490728 1105 98-99 -0.7989580744835827 1105 100-101 -1.483183220012517 1106 1 0.08584656988045225 1106 2 0.08657416843585963 1106 3 0.12725466273300157 1106 4 0.1263609293613399 1106 5 0.12897081331110627 1106 6 0.10126478131937233 1106 7 0.07570173073588649 1106 8 0.09472775860601246 1106 9 0.08198203709789453 1106 10-11 0.13529444328187168 1106 12-13 0.1416531227523805 1106 14-15 0.18381875261983538 1106 16-17 0.15282424755479695 1106 18-19 0.10940764679469339 1106 20-21 0.13434807262591875 1106 22-23 0.21495093904437113 1106 24-25 0.24544935390509437 1106 26-27 0.26018692174726965 1106 28-29 0.22861487753029763 1106 30-31 0.11240868310547114 1106 32-33 0.14817578323694391 1106 34-35 0.08232066083815681 1106 36-37 0.12574623433811638 1106 38-39 0.22690031147528344 1106 40-41 0.2229989514426265 1106 42-43 0.19974001426221832 1106 44-45 0.2569513529811829 1106 46-47 0.15094874193859198 1106 48-49 -0.008316893550663451 1106 50-51 0.022702783590574427 1106 52-53 0.043133961884194605 1106 54-55 0.11751805940497917 1106 56-57 0.06621879806708364 1106 58-59 -0.13156243817886093 1106 60-61 -0.018435590267685598 1106 62-63 -0.06548008719273213 1106 64-65 0.09047299653714447 1106 66-67 0.060659445306932014 1106 68-69 0.08490319119203349 1106 70-71 0.1345263541239703 1106 72-73 0.17575924722157765 1106 74-75 0.0669866961398995 1106 76-77 0.08835902419554742 1106 78-79 0.14530897735797055 1106 80-81 0.3523422587691165 1106 82-83 0.06892026379637883 1106 84-85 0.06528152389001463 1106 86-87 0.04570328659329448 1106 88-89 -0.11594330328779634 1106 90-91 0.07372349025228075 1106 92-93 0.25311069793385954 1106 94-95 0.19346508541060814 1106 96-97 -0.14931902086215132 1106 98-99 -0.010287273628907911 1106 100-101 -0.5714860798963137 1107 1 0.05373103604306806 1107 2 0.003874103666106521 1107 3 0.008826066569163515 1107 4 -0.004761895882182898 1107 5 0.016607487096031548 1107 6 0.05315432645618756 1107 7 0.044255056758572664 1107 8 0.014690948868747 1107 9 0.015626393472587097 1107 10-11 0.0998413436326544 1107 12-13 0.028528912334067513 1107 14-15 0.04006229969333219 1107 16-17 0.05635900838770169 1107 18-19 0.11313639622555627 1107 20-21 0.17927743512487382 1107 22-23 0.07538111276837611 1107 24-25 0.09397247542212028 1107 26-27 0.04866175453230426 1107 28-29 0.03197456766157103 1107 30-31 0.06213934356449613 1107 32-33 0.10682735406361843 1107 34-35 0.04583637551780839 1107 36-37 0.09292721127111037 1107 38-39 0.1343725598832961 1107 40-41 0.22560000186683737 1107 42-43 0.14943283892858972 1107 44-45 0.15952033917432118 1107 46-47 0.175826416897074 1107 48-49 0.1728341482162108 1107 50-51 0.2154575499385203 1107 52-53 0.34532907914309874 1107 54-55 0.2537147285726533 1107 56-57 0.13189660384762902 1107 58-59 0.18163327547473784 1107 60-61 0.0807499782631993 1107 62-63 0.09851081939132911 1107 64-65 0.08283305220065529 1107 66-67 0.07591913351433988 1107 68-69 -0.054975734271451415 1107 70-71 -0.10775867123883387 1107 72-73 -0.20329671768732638 1107 74-75 -0.10580263544914104 1107 76-77 -0.05235389191801687 1107 78-79 0.12080453004912073 1107 80-81 0.19224683561745692 1107 82-83 0.042319136418015546 1107 84-85 0.1150378357339008 1107 86-87 0.05301393127672327 1107 88-89 0.01326721121485619 1107 90-91 0.38564913971327996 1107 92-93 0.46049348713718885 1107 94-95 0.611117085897984 1107 96-97 0.3141463529747206 1107 98-99 0.05308575522249015 1107 100-101 -1.0380453313889042 1108 1 -0.045327763800898424 1108 2 0.011901987349880017 1108 3 0.021809721395328552 1108 4 0.033308895455753884 1108 5 -0.042105534936609956 1108 6 0.05927653148179246 1108 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-0.17267002872546655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 10.0 5 43.0 6 83.0 7 216.0 8 433.0 9 844.0 10 1488.0 11 2535.0 12 4076.0 13 5999.0 14 7206.0 15 9758.0 16 13740.0 17 20842.0 18 20616.0 19 12526.0 20 10309.0 21 10817.0 22 11609.0 23 12256.0 24 13777.0 25 16173.0 26 19034.0 27 22462.0 28 27288.0 29 33401.0 30 40791.0 31 49943.0 32 63488.0 33 82291.0 34 116779.0 35 176377.0 36 283131.0 37 455135.0 38 312955.0 39 16188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.64155324160756 19.537235401797123 14.452674870664092 21.368536485931223 2 15.120730812829233 21.93793425351737 39.815429752617185 23.125905181036206 3 20.82075081683003 25.935267053410684 30.002160432086416 23.24182169767287 4 12.440941521637662 17.008735080349403 38.197132759885314 32.35319063812763 5 12.678535707141428 39.13614722944589 33.793265319730615 14.39205174368207 6 29.865333066613324 36.975395079015804 17.700126692005067 15.459145162365806 7 27.315383076615323 34.39205174368207 20.79429219177169 17.498272987930918 8 25.699273187970928 34.068146962725876 19.880349403213977 20.352230446089216 9 26.293365339734613 17.91963726078549 21.53022604520904 34.25677135427085 10-11 24.935120357404813 26.979529239181172 26.836567313462695 21.248783089951324 12-13 26.963099286523974 24.86462625858505 27.32418483696739 20.848089617923584 14-15 23.010919604611093 25.95198467948 27.78193864323779 23.255157072671114 16-17 20.776876362969062 29.316451009930738 28.19508221557423 21.71159041152597 18-19 20.475348403013935 28.51498299659932 30.707554844302194 20.30211375608455 20-21 22.16045875841835 26.3749549909982 30.38991798359672 21.07466826698673 22-23 21.161912382476494 26.812242448489698 30.420004000800162 21.605841168233646 24-25 21.688316780240523 26.81771736153027 30.74015622862072 20.75380962960849 26-27 21.44949245598916 26.32867385606219 31.681031321108104 20.540802366840545 28-29 20.340361405614455 27.93492031739681 31.03519370540775 20.689524571580982 30-31 20.96600653464026 26.950243382009738 30.73932119757285 21.344428885777155 32-33 20.890684803627394 27.07160098686404 30.856117890244718 21.181596319263853 34-35 21.79007881707434 26.740693772712227 30.715371583546446 20.75385582666699 36-37 21.52842529993226 26.40097559856392 31.29478907963502 20.775810021868796 38-39 21.094465912319478 27.259526823431806 31.117821952543622 20.528185311705098 40-41 21.15680958959395 27.46591864220329 30.639332048866642 20.737939719336115 42-43 20.596719355928062 27.704167998169062 29.892224328322612 21.80688831758026 44-45 21.279737494121918 27.059689306518344 29.891117781161704 21.769455418198035 46-47 21.87551513489666 27.756548514837753 29.367423682681704 21.000512667583884 48-49 21.713844622417206 27.604905447598888 29.41774882653026 21.263501103453645 50-51 20.825915129614856 28.03834287797765 29.963088351906514 21.17265364050098 52-53 21.50067346802694 28.62233780089351 28.63844768953791 21.23854104154164 54-55 20.706754684270187 27.749469893978794 29.261265586450623 22.28250983530039 56-57 21.559407294001858 27.77583220088311 28.80787998650395 21.85688051861108 58-59 22.362854567815816 27.422898412104846 28.70987419372305 21.504372826356292 60-61 21.477058557743963 27.757088665869688 29.8245150772681 20.941337699118254 62-63 21.15444423047276 27.782848491864126 29.62129500762563 21.441412270037485 64-65 20.80926443527077 28.56476417347691 29.079016401925458 21.546954989326867 66-67 21.182961727928458 27.83622458546302 28.495008112434096 22.485805574174428 68-69 20.871301770018434 28.060362912593757 28.826875665000557 22.241459652387256 70-71 21.612075187464562 27.88190252856444 28.518484536340942 21.987537747630054 72-73 21.495339067813564 27.28572381142895 29.303407348136297 21.91552977262119 74-75 20.691231579649262 28.904980996199242 28.47470827498833 21.929079149163165 76-77 21.017335861424172 27.71092089321842 28.6249154351479 22.64682781020951 78-79 21.147039553713576 27.857928669293052 28.770370141150458 22.224661635842914 80-81 21.39820139116528 27.837415192608834 28.57575326780345 22.188630148422437 82-83 21.17212775888511 27.72191771687671 28.965073014602922 22.14088150963526 84-85 20.844248849769954 28.010908848436355 28.700246716009868 22.444595585783826 86-87 20.803717805629578 28.099871302620404 28.535920453271103 22.560490438478915 88-89 20.775408245643266 27.908745186041518 28.120374541907218 23.195472026408 90-91 20.982150271155806 27.3288246950803 28.384706416184386 23.30431861757951 92-93 21.37723544708942 27.917503500700143 28.33262652530506 22.37263452690538 94-95 20.937574181502967 27.575275055011 28.465693138627724 23.021457624858304 96-97 20.191222346454545 27.517518179645624 29.089059992830563 23.20219948106927 98-99 20.748482758363167 27.77148316537921 27.893961386287025 23.586072689970603 100-101 20.9472108873294 26.459156111413545 28.768798148104192 23.824834853152865 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1862.0 1 1249.5 2 903.0 3 1179.0 4 969.5 5 914.0 6 1658.5 7 3165.0 8 4791.0 9 5315.0 10 4345.5 11 3374.5 12 3345.0 13 3531.0 14 3658.5 15 3783.5 16 3890.0 17 3914.5 18 4069.5 19 4372.5 20 4752.0 21 5462.5 22 6421.5 23 7575.5 24 8803.0 25 10524.5 26 13370.0 27 16436.0 28 18730.5 29 21105.5 30 24232.5 31 27941.0 32 32185.0 33 36386.5 34 40509.5 35 43249.5 36 46764.5 37 51492.5 38 52964.0 39 53074.5 40 54006.5 41 55611.5 42 57173.0 43 59064.0 44 61965.5 45 64253.0 46 66308.0 47 78043.0 48 105821.5 49 103296.5 50 75857.5 51 64142.0 52 57453.5 53 53716.0 54 50412.5 55 46016.0 56 40977.0 57 35605.5 58 31507.5 59 27865.0 60 23661.0 61 19362.5 62 15571.5 63 11590.5 64 8165.5 65 6217.5 66 4731.5 67 3695.0 68 2701.5 69 1812.5 70 1393.0 71 1124.5 72 918.5 73 870.0 74 751.0 75 608.5 76 427.5 77 248.5 78 145.5 79 80.0 80 43.5 81 24.0 82 16.0 83 14.5 84 7.0 85 3.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4253650730146029 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.001600320064013E-4 16-17 0.0021871040874841636 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.6672001066880042E-5 26-27 0.003120624124824965 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011815696472627858 36-37 0.026325265053010603 38-39 0.02013736080549443 40-41 0.009415216376608655 42-43 0.009388544375541775 44-45 0.004080816163232646 46-47 0.00618790424751617 48-49 0.002987264119490565 50-51 7.201440288057612E-4 52-53 0.0 54-55 0.0 56-57 1.3336000533440022E-4 58-59 0.0019470560778822431 60-61 0.002560512102420484 62-63 0.0013869440554777623 64-65 0.003120624124824965 66-67 0.001787024071480963 68-69 0.005147696205907848 70-71 2.1337600853504033E-4 72-73 0.0 74-75 0.0 76-77 0.09612589184503567 78-79 0.016696672667866907 80-81 5.067680202707208E-4 82-83 0.0 84-85 0.0 86-87 0.10748816429952657 88-89 0.42237780889511234 90-91 0.42600520104020806 92-93 0.0 94-95 0.0 96-97 5.3344002133760084E-5 98-99 3.200640128025605E-4 100-101 2.933920117356805E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1874625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.76003003058681 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21844239753973 38.41548807320034 2 14.539341384840249 15.0511349340857 3 5.08388001861455 7.89425347306168 4 2.205074103168908 4.565388071987678 5 1.164549506394577 3.013855871154418 6 0.679492777165778 2.11023399310005 7 0.43072313474872065 1.5605969672623587 8 0.28747708174019354 1.1903857907182303 9 0.20922272755992477 0.9746437195424689 >10 1.037825889508063 9.882140920533502 >50 0.09139373397514872 3.2362614659324636 >100 0.04671317458766208 4.421655708783014 >500 0.0020575684759903466 0.680320761413107 >1k 0.0030863527139855197 3.43222250132137 >5k 5.143921189975867E-4 1.9689151654077672 >10k+ 2.0575684759903463E-4 1.6025025824957768 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17909 0.9553377342135093 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12185 0.6499966659998666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9880 0.5270387410815497 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8382 0.44712942588517707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6572 0.35057678202307124 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6177 0.32950590118023604 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5964 0.31814362872574514 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4708 0.2511435620457425 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3918 0.20900180036007204 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3744 0.19971994398879775 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3743 0.199666599986664 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3718 0.19833299993332 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3291 0.17555511102220445 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3023 0.16125891845035673 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2844 0.15171034206841366 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2639 0.14077482163099286 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2617 0.13960125358405015 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2460 0.1312262452490498 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2268 0.12098419683936787 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2208 0.11778355671134227 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0668800426752017E-4 0.0 3 0.0 0.0 0.0 1.0668800426752017E-4 0.0 4 0.0 0.0 0.0 1.0668800426752017E-4 0.0 5 0.0 0.0 0.0 1.0668800426752017E-4 0.0 6 0.0 0.0 0.0 1.0668800426752017E-4 0.0 7 0.0 0.0 0.0 1.0668800426752017E-4 0.0 8 0.0 0.0 0.0 1.0668800426752017E-4 0.0 9 0.0 0.0 0.0 1.0668800426752017E-4 0.0 10-11 0.0 0.0 0.0 1.6003200640128025E-4 0.0 12-13 0.0 0.0 0.0 2.6672001066880045E-4 0.0 14-15 0.0 0.0 0.0 2.6672001066880045E-4 0.0 16-17 0.0 0.0 0.0 3.734080149363206E-4 0.0 18-19 0.0 0.0 0.0 6.40128025605121E-4 0.0 20-21 0.0 0.0 0.0 7.734880309395213E-4 0.0 22-23 0.0 0.0 0.0 0.001253584050143362 0.0 24-25 0.0 0.0 0.0 0.002000400080016003 0.0 26-27 0.0 0.0 0.0 0.0030672801226912048 0.0 28-29 0.0 0.0 0.0 0.00642795225711809 0.0 30-31 0.0 0.0 0.0 0.017310128692405148 0.0 32-33 2.6672001066880042E-5 0.0 0.0 0.036273921450956856 0.0 34-35 5.3344002133760084E-5 0.0 0.0 0.06595985863839435 0.0 36-37 5.3344002133760084E-5 0.0 0.0 0.10927518837100753 0.0 38-39 5.3344002133760084E-5 0.0 0.0 0.17819563912782554 0.0 40-41 5.3344002133760084E-5 0.0 0.0 0.2566913382676535 0.0 42-43 5.3344002133760084E-5 0.0 0.0 0.33620057344802295 0.0 44-45 5.3344002133760084E-5 0.0 0.0 0.43398012935920516 0.0 46-47 5.3344002133760084E-5 0.0 0.0 0.5524838300993532 0.0 48-49 5.3344002133760084E-5 0.0 0.0 0.677708875108355 0.0 50-51 5.3344002133760084E-5 0.0 0.0 0.8151496966059879 0.0 52-53 5.3344002133760084E-5 0.0 0.0 0.9533906781356272 0.0 54-55 5.3344002133760084E-5 0.0 0.0 1.1060345402413816 0.0 56-57 5.3344002133760084E-5 0.0 0.0 1.2968193638727745 0.0 58-59 5.3344002133760084E-5 0.0 0.0 1.5077948923117956 0.0 60-61 5.3344002133760084E-5 0.0 0.0 1.7430686137227447 0.0 62-63 5.3344002133760084E-5 0.0 0.0 1.9742348469693938 0.0 64-65 5.3344002133760084E-5 0.0 0.0 2.2103620724144832 0.0 66-67 5.3344002133760084E-5 0.0 0.0 2.488897779555911 0.0 68-69 5.3344002133760084E-5 0.0 0.0 2.8134160165366406 0.0 70-71 5.3344002133760084E-5 0.0 0.0 3.1735147029405884 0.0 72-73 5.3344002133760084E-5 0.0 0.0 3.532173101286924 0.0 74-75 5.3344002133760084E-5 0.0 0.0 3.8962459158498364 0.0 76-77 5.3344002133760084E-5 0.0 0.0 4.284990331399614 0.0 78-79 5.3344002133760084E-5 0.0 0.0 4.762739214509569 0.0 80-81 5.3344002133760084E-5 0.0 0.0 5.288017603520704 0.0 82-83 5.3344002133760084E-5 0.0 0.0 5.794865639794626 0.0 84-85 5.3344002133760084E-5 0.0 0.0 6.312169100486764 0.0 86-87 5.3344002133760084E-5 0.0 0.0 6.86043875441755 0.0 88-89 5.3344002133760084E-5 0.0 0.0 7.443195305727812 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 480 0.0 32.624847 94-95 GTACATG 12450 0.0 24.572666 1 TACATGG 12475 0.0 23.964909 2 ACATGGG 12555 0.0 22.905075 3 CGCGGAA 105 3.2971002E-8 22.716965 82-83 GAGTACT 10340 0.0 22.030262 12-13 GTGTAGC 990 0.0 21.660273 1 AGTACTT 10500 0.0 20.768024 12-13 GTACTTT 10985 0.0 20.715122 14-15 TACCTGG 1395 0.0 20.410461 2 CATGGGA 8325 0.0 20.29278 4 ATGGGCG 235 1.1443808E-7 20.193329 5 ATTCGCG 120 1.4137368E-7 19.793753 80-81 GTATAGG 510 0.0 19.62166 1 CTCCGAC 425 0.0 19.540016 94-95 CATGGGG 3695 0.0 19.52113 4 CCCGTAG 130 0.007426204 18.327923 1 ATCACGC 130 0.0076069464 18.251663 3 ATGGGAG 2835 0.0 18.077837 5 ACTTTTT 12675 0.0 17.989828 16-17 >>END_MODULE