FastQCFastQC Report
Fri 27 May 2016
SRR939199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664461
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95970.36018541836416446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79590.298709570153213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48980.1838270479470332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73650.038.6761781
GTATCAA112450.034.2394261
ATCAACG134600.028.2978123
TATCAAC137450.027.9523282
TCAACGC137350.027.6967454
CAACGCA139650.027.274515
AACGCAG144900.026.3086176
TACCTGG23750.024.7343182
GTACATG115900.023.7579861
ACGCAGA163450.023.293877
TACATGG116350.023.127242
CGCAGAG165100.022.9463398
GAGTACT83800.022.46278812-13
GTACCTG27200.022.341181
ACATGGG119300.021.7214473
GCAGAGT178500.020.8776959
TATAGCG1856.7096043E-620.486145
CATGGGG58100.020.4663054
GTACTTT92150.020.19743714-15
AGTACTT88450.019.48830412-13
Produced by FastQC (version 0.11.5)