FastQCFastQC Report
Fri 27 May 2016
SRR939199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664461
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.35947232854975175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82910.3111698763840041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50150.18821817996210113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.039.1261861
GTATCAA112000.033.6260871
ATCAACG137250.027.067033
TCAACGC139050.026.7166484
TATCAAC140050.026.6952692
CAACGCA141200.026.3098435
AACGCAG145800.025.5123066
GTACATG112700.023.7004621
TACATGG112850.023.1234342
ACGCAGA164450.022.5035977
ACATGGG113800.022.2633343
CGCAGAG166550.022.1628788
TACCTGG22550.022.0919462
GAGTACT89550.022.04078712-13
GTACTTT95750.020.514514-15
GCAGAGT179750.020.324189
GTACCTG25950.020.182421
CATGGGG58550.019.6911284
AGTACTT92850.019.18789712-13
GTGTAGC14000.019.0448631
Produced by FastQC (version 0.11.5)