FastQCFastQC Report
Fri 27 May 2016
SRR939198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621063
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93660.3573359358397719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76210.29075989398194546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47540.18137679254561984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.037.5360341
GTATCAA104750.035.9962581
TATCAAC129550.028.8841232
ATCAACG129750.028.7665883
CAACGCA132050.028.4079385
TCAACGC131500.028.3837624
AACGCAG134900.027.7918436
GTACATG111900.025.5055851
TACATGG112050.024.4757612
ACGCAGA155050.024.0579477
CGCAGAG154800.024.0356438
ACATGGG112850.023.4208223
GCAGAGT166550.022.0841439
TAAGGTG8800.021.5293185
GAGTACT85400.021.45291112-13
GTATAGG6050.021.1933351
GTACTTT89100.020.50911314-15
CATGGGG57000.020.3592014
AGTACTT88300.019.75649612-13
AGAGTAC148650.019.4418410-11
Produced by FastQC (version 0.11.5)