FastQCFastQC Report
Fri 27 May 2016
SRR939198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621063
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93720.35756485059687615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80490.3070891466553837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49620.18931250412523468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70800.038.769291
GTATCAA109850.033.359911
ATCAACG131400.027.664463
TCAACGC133250.027.2803754
TATCAAC133900.027.1479452
CAACGCA135500.026.6872885
AACGCAG138800.026.0527946
GTACATG108000.025.8566631
TACATGG108950.025.1325972
ACATGGG109150.023.956133
TACCTGG21350.023.7834422
ACGCAGA157600.022.8245287
CGCAGAG158900.022.5481978
CATGGGG56800.022.2239934
GAGTACT89300.022.08173612-13
GTACTTT93550.020.92636514-15
GCAGAGT173450.020.5750359
GTGTAGC13650.019.5503271
GTACCTG26500.019.2413941
AGTACTT92700.019.0192212-13
Produced by FastQC (version 0.11.5)