##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939198_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2621063 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50833459554387 34.0 31.0 34.0 31.0 34.0 2 32.72407340075382 34.0 31.0 34.0 31.0 34.0 3 32.83030510903401 34.0 31.0 34.0 31.0 34.0 4 36.27838056544234 37.0 37.0 37.0 35.0 37.0 5 36.239415077012644 37.0 37.0 37.0 35.0 37.0 6 36.277217296951655 37.0 37.0 37.0 35.0 37.0 7 36.23231528582106 37.0 37.0 37.0 35.0 37.0 8 36.206701632124066 37.0 37.0 37.0 35.0 37.0 9 37.95487136325987 39.0 38.0 39.0 35.0 39.0 10-11 37.868597969602405 39.0 38.0 39.0 35.0 39.0 12-13 37.77697235816156 39.0 38.0 39.0 35.0 39.0 14-15 39.20028133623648 40.5 38.5 41.0 36.0 41.0 16-17 39.29920169793706 41.0 39.0 41.0 36.0 41.0 18-19 39.322404497717145 41.0 39.0 41.0 36.0 41.0 20-21 39.28871778358628 41.0 39.0 41.0 36.0 41.0 22-23 39.2184934127871 41.0 39.0 41.0 36.0 41.0 24-25 39.145280178309335 41.0 39.0 41.0 36.0 41.0 26-27 39.00484459930952 40.0 39.0 41.0 35.0 41.0 28-29 38.848353320771 40.0 38.0 41.0 35.0 41.0 30-31 38.65866444263263 40.0 38.0 41.0 35.0 41.0 32-33 38.573195112059494 40.0 38.0 41.0 35.0 41.0 34-35 38.471704609923535 40.0 38.0 41.0 34.0 41.0 36-37 38.323893588212115 40.0 38.0 41.0 34.0 41.0 38-39 38.19877584018393 40.0 38.0 41.0 34.0 41.0 40-41 38.00886090872291 40.0 38.0 41.0 33.0 41.0 42-43 37.81761998853137 40.0 37.5 41.0 33.0 41.0 44-45 37.55909110158741 40.0 37.0 41.0 33.0 41.0 46-47 37.23755647994726 40.0 37.0 41.0 32.0 41.0 48-49 36.89159112161745 40.0 36.5 41.0 31.0 41.0 50-51 36.62784564888368 39.5 36.0 41.0 31.0 41.0 52-53 36.34361573911043 39.0 35.5 41.0 30.0 41.0 54-55 36.08032752360397 39.0 35.0 40.0 30.0 41.0 56-57 35.818891800769386 39.0 35.0 40.0 29.0 41.0 58-59 35.466815753760976 38.0 35.0 40.0 28.0 41.0 60-61 35.0839140455609 38.0 34.0 40.0 28.0 41.0 62-63 34.674268607812934 37.5 34.0 40.0 26.5 41.0 64-65 34.200957397819124 37.0 34.0 39.5 26.0 41.0 66-67 33.76449917457154 36.0 33.0 39.0 26.0 40.5 68-69 33.25954736685078 36.0 33.0 39.0 24.5 40.0 70-71 32.73432763729831 35.0 32.0 38.0 23.5 40.0 72-73 32.13856629924577 35.0 31.5 37.5 21.5 39.5 74-75 31.57306443988565 35.0 31.0 37.0 20.0 39.0 76-77 29.590031792444517 32.5 28.5 35.0 17.5 37.0 78-79 30.579812274638194 34.0 30.0 36.0 18.0 37.5 80-81 30.582764511955645 34.0 31.0 35.5 17.5 37.0 82-83 30.18448335656182 34.0 30.0 35.0 12.5 37.0 84-85 29.6978132536303 34.0 30.0 35.0 7.0 36.0 86-87 28.971031409775346 34.0 29.0 35.0 2.0 36.0 88-89 28.281702118567924 33.0 28.0 35.0 2.0 35.0 90-91 27.706839934789816 33.0 26.5 35.0 2.0 35.0 92-93 27.221802566363344 33.0 26.0 34.5 2.0 35.0 94-95 26.6432119334789 32.0 25.0 34.0 2.0 35.0 96-97 25.798629029519702 32.0 24.5 34.0 2.0 35.0 98-99 24.53358389325247 32.0 10.5 34.0 2.0 35.0 100-101 18.66830060933293 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11071981637467587 1101 2 0.13224374405564987 1101 3 0.11997496153772147 1101 4 0.1521571366386567 1101 5 0.10956747352446428 1101 6 0.08984582150003462 1101 7 0.1814652923763731 1101 8 0.15899006091508738 1101 9 0.1593527819820153 1101 10-11 0.21144924575107638 1101 12-13 0.12568362135821332 1101 14-15 0.12462550461768984 1101 16-17 0.17762256401454835 1101 18-19 0.2576863888785752 1101 20-21 0.20216455429815738 1101 22-23 0.3025771351914486 1101 24-25 0.24890281826292693 1101 26-27 0.20184446145500345 1101 28-29 0.21607311225236714 1101 30-31 0.21965642907532157 1101 32-33 0.26427432647372484 1101 34-35 0.2328177737689856 1101 36-37 0.4184454200592498 1101 38-39 0.27341268335293734 1101 40-41 0.31307107701554315 1101 42-43 0.31127781094529183 1101 44-45 0.35148234357925645 1101 46-47 0.32185635333097196 1101 48-49 0.27392072975895587 1101 50-51 0.2484791057703788 1101 52-53 0.27502940749203475 1101 54-55 0.22310743120410592 1101 56-57 0.26520559546764844 1101 58-59 0.2410580274313716 1101 60-61 0.23441989891576043 1101 62-63 0.20033139926877652 1101 64-65 0.16325614278910194 1101 66-67 0.0026602172558156667 1101 68-69 0.14341722573161064 1101 70-71 0.07325512274791635 1101 72-73 0.19893411086822965 1101 74-75 -0.11851922990628694 1101 76-77 -0.014336191570347268 1101 78-79 0.2603878782909206 1101 80-81 0.28480852679646773 1101 82-83 0.0973645804931671 1101 84-85 -0.016242344111240925 1101 86-87 0.12208533186730364 1101 88-89 0.05095683981931742 1101 90-91 0.2268000198536697 1101 92-93 0.13631952922075996 1101 94-95 0.11540749508595027 1101 96-97 0.05467472629741721 1101 98-99 -0.1307088666860281 1101 100-101 -0.5771130126739834 1102 1 0.16307353392857493 1102 2 0.13444348008732732 1102 3 0.14109242744181216 1102 4 0.07560632273632706 1102 5 0.15884156063401633 1102 6 0.19015378454447074 1102 7 0.2074221775501499 1102 8 0.11719507631323722 1102 9 0.23238401823366672 1102 10-11 0.20924950971939893 1102 12-13 0.22511168998997988 1102 14-15 0.30170425516762833 1102 16-17 0.2616524804245799 1102 18-19 0.1925742023409569 1102 20-21 0.2536383774393798 1102 22-23 0.2284460309239691 1102 24-25 0.22338588029548134 1102 26-27 0.17742739150339304 1102 28-29 0.2668870145840856 1102 30-31 0.15762387298205027 1102 32-33 0.22665884033205685 1102 34-35 0.1921226571829706 1102 36-37 0.2110103122721867 1102 38-39 0.2091803912279957 1102 40-41 0.2272813717801725 1102 42-43 0.21448942555154105 1102 44-45 0.2947291539620167 1102 46-47 0.3420939248223931 1102 48-49 0.2499436070136838 1102 50-51 0.07997932574397737 1102 52-53 0.2334543964933573 1102 54-55 0.2242072992199411 1102 56-57 0.1994334881205262 1102 58-59 0.032523051628473354 1102 60-61 0.05162183468346626 1102 62-63 -0.013482943010146187 1102 64-65 0.03149195449169895 1102 66-67 0.11352691325230779 1102 68-69 0.1775131342225862 1102 70-71 0.1469462798090717 1102 72-73 0.06123063528529826 1102 74-75 0.17668534554465865 1102 76-77 0.04439676047540786 1102 78-79 0.35541647485933225 1102 80-81 0.3109853855734137 1102 82-83 0.1527979284914096 1102 84-85 0.18877305404098266 1102 86-87 0.3427188558444243 1102 88-89 0.22451601271857058 1102 90-91 0.5316834338439911 1102 92-93 0.23024825777332936 1102 94-95 0.3294418109680457 1102 96-97 0.4913223285851416 1102 98-99 0.48719698461181693 1102 100-101 -0.3100650584284921 1103 1 0.05781041637376205 1103 2 0.047773305595377735 1103 3 0.0942083496983912 1103 4 0.06239736774124083 1103 5 0.09904668027052566 1103 6 0.05909627102797543 1103 7 0.08011781992672695 1103 8 0.0720955056833219 1103 9 0.07706425939218065 1103 10-11 0.01086608576511594 1103 12-13 0.06887582156945626 1103 14-15 -0.054924116675138634 1103 16-17 0.10238431037502238 1103 18-19 0.13343253627468243 1103 20-21 0.11213518360660402 1103 22-23 0.13101544907462426 1103 24-25 0.13759301341860208 1103 26-27 0.1612891277466133 1103 28-29 0.14943174510492696 1103 30-31 0.10909468777347087 1103 32-33 0.08731332311816686 1103 34-35 0.11738001480179605 1103 36-37 0.11338869062984713 1103 38-39 0.1531793848679257 1103 40-41 0.28124187387183497 1103 42-43 0.22758312434253725 1103 44-45 0.19824792683122183 1103 46-47 0.3113820266264895 1103 48-49 0.056331962362264676 1103 50-51 0.1463971744174657 1103 52-53 -0.022920143439897345 1103 54-55 -0.06709360704064693 1103 56-57 0.024820434718293427 1103 58-59 0.0118583711113871 1103 60-61 0.11398473441076362 1103 62-63 -0.05861987849426242 1103 64-65 -0.001670982332193205 1103 66-67 -0.06688650682369968 1103 68-69 -0.16686646357062784 1103 70-71 -0.21701202254656238 1103 72-73 -0.31535708359610126 1103 74-75 -0.28942865979908916 1103 76-77 -0.2695994624168705 1103 78-79 -0.2689400831899391 1103 80-81 -0.058362078445188814 1103 82-83 -0.09946743555340731 1103 84-85 -0.3543174371209865 1103 86-87 -0.2649975039066881 1103 88-89 -0.03947492160972743 1103 90-91 -0.013738911641798524 1103 92-93 -0.05390456026481161 1103 94-95 -0.22586995754307893 1103 96-97 -0.342034637220074 1103 98-99 -0.911541098873915 1103 100-101 -1.067658650681743 1104 1 0.07152698573057137 1104 2 0.10083415172024957 1104 3 0.13537571479868404 1104 4 0.04630004219269779 1104 5 0.07549367386690875 1104 6 0.04486029070967845 1104 7 0.09994519303153737 1104 8 0.11205189281958639 1104 9 0.07273092998263309 1104 10-11 0.13124582962047526 1104 12-13 0.15800924397269256 1104 14-15 0.13581889114103518 1104 16-17 0.15884928471346882 1104 18-19 0.15424649592248585 1104 20-21 0.20391421764936268 1104 22-23 0.14052449535957834 1104 24-25 0.1977975995378216 1104 26-27 0.16336162162550494 1104 28-29 0.19229933503806507 1104 30-31 0.17019757528642998 1104 32-33 0.22994460313511667 1104 34-35 0.2210341371297062 1104 36-37 0.3874162297107375 1104 38-39 0.2646585142586346 1104 40-41 0.280818840864967 1104 42-43 0.3222027330627455 1104 44-45 0.36894358437901076 1104 46-47 0.3573447467507904 1104 48-49 0.2760604560999056 1104 50-51 0.2477426400922056 1104 52-53 0.17036841951330217 1104 54-55 0.10012435133584319 1104 56-57 0.21196287986378337 1104 58-59 0.06043359594423947 1104 60-61 0.11021275140859643 1104 62-63 0.16192157432615062 1104 64-65 0.1485233921380953 1104 66-67 0.1698251067934038 1104 68-69 0.05096022091002794 1104 70-71 0.06888398343328106 1104 72-73 0.0767488997827499 1104 74-75 -0.0765238723897319 1104 76-77 -0.22341970796730237 1104 78-79 0.13818761833562831 1104 80-81 0.1516403346822237 1104 82-83 0.13975956910804754 1104 84-85 -0.042423661812573954 1104 86-87 0.1080116659774788 1104 88-89 0.2706987647072481 1104 90-91 0.19995014217682794 1104 92-93 0.17209217907034358 1104 94-95 0.05218338434410086 1104 96-97 -0.22239354578020354 1104 98-99 -0.29730747282626524 1104 100-101 -0.7690620902711593 1105 1 0.07367095662453949 1105 2 0.09629283155392443 1105 3 0.041313864486831164 1105 4 0.09357135196400179 1105 5 0.05309892334716437 1105 6 0.1050798853042707 1105 7 0.105941814653157 1105 8 0.046374917767607826 1105 9 0.1028699632466683 1105 10-11 0.20512836600300943 1105 12-13 0.15910396850491537 1105 14-15 0.19699352731567643 1105 16-17 0.18758174313342835 1105 18-19 0.22832069030918234 1105 20-21 0.24013108355372736 1105 22-23 0.2012475982701858 1105 24-25 0.16827577001598826 1105 26-27 0.17612474532612765 1105 28-29 0.07905544210667159 1105 30-31 0.20441340168975586 1105 32-33 0.19103153140954987 1105 34-35 0.18985560282617087 1105 36-37 0.20086152596853424 1105 38-39 0.15914266186778292 1105 40-41 0.234538305521923 1105 42-43 0.03600860624361957 1105 44-45 -0.024510807512875488 1105 46-47 0.14274625402730123 1105 48-49 0.11100718167162427 1105 50-51 0.15935906420862267 1105 52-53 0.14171717211204538 1105 54-55 0.05043620164769891 1105 56-57 0.07922509400100353 1105 58-59 -0.029194132745992363 1105 60-61 -0.13773098559764207 1105 62-63 -0.21518666371958517 1105 64-65 -0.12599259019038556 1105 66-67 -0.10719542335213106 1105 68-69 -0.17108694644964828 1105 70-71 -0.19832300721120788 1105 72-73 -0.3699669266205774 1105 74-75 -0.20837483392819323 1105 76-77 -0.28126469237478346 1105 78-79 -0.27972753239201964 1105 80-81 -0.1335662661493764 1105 82-83 -0.008560987025006739 1105 84-85 -0.23353592792484434 1105 86-87 -0.23560189951108512 1105 88-89 -0.26361126647778477 1105 90-91 -0.18202426011007233 1105 92-93 -0.19797668787352407 1105 94-95 -0.31415302417980584 1105 96-97 -0.46014042727708215 1105 98-99 -0.5924802898740822 1105 100-101 -1.2031122461963122 1106 1 -0.042278895534352046 1106 2 0.042793408000584066 1106 3 0.04408908650717791 1106 4 0.036632219039802294 1106 5 0.024152936873178987 1106 6 0.03774884626024999 1106 7 0.03374120259356772 1106 8 0.09011432766214256 1106 9 0.20348107430987028 1106 10-11 0.10952738263761574 1106 12-13 0.14638696026737819 1106 14-15 0.1325161794704286 1106 16-17 0.08949225205347489 1106 18-19 0.09210319789094257 1106 20-21 0.16639325166574537 1106 22-23 0.25407701576157393 1106 24-25 0.16287881513749625 1106 26-27 0.2108903854837294 1106 28-29 0.24840348395574807 1106 30-31 0.19141148997361057 1106 32-33 0.27306122725093473 1106 34-35 0.23229078599133857 1106 36-37 0.13455429412832842 1106 38-39 0.32387559503705177 1106 40-41 0.2079366504258715 1106 42-43 0.2532197426584091 1106 44-45 0.2440757383859875 1106 46-47 0.2284120046453495 1106 48-49 0.1057320695943531 1106 50-51 0.173912251221374 1106 52-53 0.12424010278927256 1106 54-55 0.07801846278450597 1106 56-57 0.0022251000370943075 1106 58-59 0.01648131010904308 1106 60-61 0.0983291373144084 1106 62-63 0.0038101207686978 1106 64-65 0.11658004338183758 1106 66-67 0.12622899746341432 1106 68-69 -0.03865166843162626 1106 70-71 -0.06370887292888483 1106 72-73 -0.002590358207093857 1106 74-75 -0.1450427911685246 1106 76-77 -0.10413578476060081 1106 78-79 0.18924644429659665 1106 80-81 0.35738494412991173 1106 82-83 0.18195277914201569 1106 84-85 0.19950942639799152 1106 86-87 0.13102222708815603 1106 88-89 0.09832807770959207 1106 90-91 0.3179803100728442 1106 92-93 0.2684111688917703 1106 94-95 0.31348373423256604 1106 96-97 0.03950749617838767 1106 98-99 -0.0040995056824293385 1106 100-101 -0.8430842732276531 1107 1 -0.03651037626421072 1107 2 -0.041760004315506194 1107 3 0.01516465667823752 1107 4 0.027146286412303766 1107 5 -0.05011135628232921 1107 6 -0.02726134834700389 1107 7 -0.006181185679444923 1107 8 -0.04744857256243051 1107 9 0.017476706674372622 1107 10-11 0.050152007533199594 1107 12-13 0.038796929857262796 1107 14-15 0.010156358819116917 1107 16-17 0.09731517033410597 1107 18-19 0.015344465542561636 1107 20-21 0.055392559950576015 1107 22-23 0.06351944656823605 1107 24-25 0.10268263542722167 1107 26-27 0.07651347497250782 1107 28-29 0.014015525101690685 1107 30-31 -0.0026547101818508168 1107 32-33 -0.05025535354546662 1107 34-35 -0.017800885616345852 1107 36-37 0.02362135789866926 1107 38-39 0.024069591423533154 1107 40-41 0.12215433636939821 1107 42-43 0.019027640791044576 1107 44-45 -0.04528052522582726 1107 46-47 0.030107258418865968 1107 48-49 0.02142893270473678 1107 50-51 -0.027752113295562708 1107 52-53 -0.0036210845799118374 1107 54-55 -0.1452701102158187 1107 56-57 -0.2045354155237007 1107 58-59 -0.21881447345803906 1107 60-61 -0.25496871069563554 1107 62-63 -0.28167189570699946 1107 64-65 -0.1253932367526076 1107 66-67 -0.04846604933891285 1107 68-69 -0.15075044358039236 1107 70-71 -0.20406382605246165 1107 72-73 -0.41000137582533114 1107 74-75 -0.5215238824484167 1107 76-77 -0.4830915566470608 1107 78-79 -0.3959526621542686 1107 80-81 -0.15719310549674148 1107 82-83 -0.32823343071491706 1107 84-85 -0.3588338095546 1107 86-87 -0.4449371057895206 1107 88-89 -0.5261839469543936 1107 90-91 -0.130531860146057 1107 92-93 -0.0069241548995933044 1107 94-95 -0.18933672163424475 1107 96-97 -0.3420889284395585 1107 98-99 -0.3675357313050682 1107 100-101 -0.9170775444410886 1108 1 -0.11845015857349495 1108 2 -0.08479358030171369 1108 3 -0.07427865110568632 1108 4 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2316 98-99 -0.2732607294432974 2316 100-101 0.9561144387319942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 21.0 5 41.0 6 87.0 7 276.0 8 524.0 9 1042.0 10 1843.0 11 3080.0 12 4533.0 13 6894.0 14 8367.0 15 11371.0 16 15205.0 17 21820.0 18 22464.0 19 16016.0 20 15047.0 21 16036.0 22 17108.0 23 18644.0 24 20988.0 25 24302.0 26 28951.0 27 34147.0 28 41398.0 29 50314.0 30 61319.0 31 75719.0 32 95134.0 33 123591.0 34 172961.0 35 258829.0 36 417240.0 37 634296.0 38 386519.0 39 14929.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24524077177544 18.991663544039767 14.387149030146567 23.375946654038227 2 15.75032725272151 20.260749169325575 39.95043232459502 24.038491253357893 3 19.642221495629826 24.10491468537765 29.549919250319434 26.702944568673093 4 13.329897068479468 16.586552860423424 35.93973132274959 34.14381874834752 5 14.210379529221543 36.92467521765025 33.64833275659532 15.216612496532894 6 32.726798249412546 35.18881461452853 17.214351581781894 14.870035554277026 7 29.205440693337014 32.33008897535084 20.11409111494077 18.350379216371373 8 26.28590003368862 35.54668468480155 19.345662427801237 18.82175285370859 9 26.57963581951292 17.050906445209442 20.220193104858602 36.14926463041903 10-11 25.48166526329203 26.52498242125428 26.61502222571529 21.3783300897384 12-13 26.045711224796964 23.778482241746957 27.563358835709025 22.612447697747058 14-15 22.82176697373366 25.101820464053322 25.978153668302788 26.09825889391023 16-17 22.519809172654185 28.555387850781074 26.481700933381358 22.443102043183387 18-19 22.28802250995541 26.817979349977346 29.000491213009994 21.893506927057253 20-21 23.023597677736092 26.073219148108993 28.9538252228199 21.94935795133501 22-23 22.706207367011018 26.014521589141502 28.87584159556638 22.4034294482811 24-25 22.607362969666493 26.175725717285587 28.993724681064393 22.223186631983523 26-27 22.37506154662349 26.54432186733171 28.866811485243787 22.213805100801018 28-29 22.145484894007677 26.604153849822353 29.083720819324228 22.166640436845743 30-31 22.28462268934398 26.24212008639243 29.049702353587076 22.423554870676515 32-33 22.152367188426986 26.366497104419086 28.935531118481318 22.54560458867261 34-35 22.757313738834004 26.40098552486506 28.62429268424837 22.217408052052562 36-37 22.653887487043743 26.228439952433945 28.665914097168475 22.451758463353837 38-39 22.38062065600802 26.44211422889855 28.904276528748408 22.27298858634502 40-41 22.530993444268653 26.281093841603624 28.854241765939996 22.333670948187727 42-43 22.44670230307558 26.339145950992847 28.55329903238355 22.660852713548024 44-45 22.507333224466873 26.607223798411873 28.37187155182018 22.513571425301073 46-47 22.530947101098697 26.851484438312596 27.92797501949694 22.68959344109177 48-49 22.82305478077218 26.740496458676677 27.87716900926694 22.559279751284205 50-51 22.605682581886725 26.970831666535993 27.749967617871423 22.673518133705855 52-53 22.88447473410597 26.934816141389962 27.574518430117855 22.606190694386207 54-55 22.51010372509169 26.777914151624742 27.745021771701023 22.966960351582543 56-57 22.52868638476678 27.306256379812677 27.629102800183357 22.535954435237187 58-59 22.720598545033475 26.76218918380136 27.738792780298528 22.778419490866636 60-61 22.76695747192482 26.686342377979688 28.060437283813265 22.48626286628223 62-63 22.55966165972919 26.805263579585127 27.924213000232733 22.710861760452953 64-65 22.414042745176214 26.683199812285206 27.842853722676196 23.059903719862383 66-67 22.350179778161685 27.04520550872031 27.651479888653522 22.95313482446448 68-69 22.301531751123495 26.81490655408173 27.87434882978701 23.00921286500777 70-71 22.30536500499798 26.917029751321987 27.750929013452573 23.026676230227466 72-73 22.461383034287998 26.76698347197301 27.664253014902734 23.10738047883626 74-75 22.31983740947852 26.95232812030844 27.439935629170304 23.287898841042736 76-77 22.317788495780192 26.878224478608047 27.574623068129284 23.22936395748247 78-79 22.29079514272991 26.94716079871818 27.566339483082242 23.195704575469662 80-81 22.08727441569796 27.199434426105984 27.563848824967202 23.14944233322886 82-83 22.304442892063257 27.032677200052042 27.43604407829953 23.22683582958517 84-85 22.24154856254886 26.786593836164947 27.38606435633176 23.585793244954434 86-87 22.193718831245963 26.629025130556904 27.701285650828982 23.47597038736815 88-89 22.105137733273413 26.62900116892924 27.600484429410425 23.66537666838692 90-91 21.993394686630754 26.823338518478785 27.676435429613566 23.506831365276895 92-93 22.066886602878295 26.588983172094682 27.57963085969319 23.764499365333837 94-95 21.869528101676323 26.48002479910342 27.616529556239122 24.03391754298114 96-97 21.949232791898858 26.419520026615167 27.633875123900157 23.99737205758582 98-99 21.749251638078974 26.37679135677272 27.480277048428537 24.39367995671977 100-101 22.094237262140105 25.41093670505516 28.247562193002857 24.24726383980188 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 812.0 1 586.0 2 482.0 3 631.5 4 527.5 5 473.5 6 857.0 7 1719.5 8 2773.0 9 3328.0 10 3127.5 11 2793.5 12 2807.0 13 3003.5 14 3268.0 15 3466.5 16 3563.5 17 3706.5 18 3952.0 19 4116.5 20 4429.0 21 5111.5 22 6120.5 23 7504.5 24 9023.5 25 11007.5 26 13716.0 27 16771.5 28 19699.0 29 23073.0 30 26913.5 31 31057.5 32 35307.5 33 39578.5 34 44393.0 35 48704.0 36 53760.0 37 59964.0 38 64326.5 39 66674.5 40 69679.5 41 76335.0 42 83332.0 43 88015.5 44 92571.0 45 97045.0 46 98487.0 47 99956.0 48 108217.0 49 111241.5 50 107395.5 51 104850.5 52 100864.5 53 98615.5 54 95592.5 55 86882.0 56 76435.5 57 67779.0 58 60467.0 59 52679.0 60 44636.0 61 36658.0 62 29584.0 63 23437.5 64 18256.5 65 14351.0 66 10796.0 67 8940.0 68 6985.5 69 4609.5 70 3306.5 71 2460.5 72 2029.5 73 1679.0 74 1374.5 75 1151.5 76 734.0 77 379.0 78 227.0 79 123.0 80 66.0 81 46.5 82 24.5 83 13.0 84 9.5 85 7.0 86 6.5 87 4.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4227674039120769 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010682688664866125 16-17 0.002098385273455846 18-19 1.907622975868951E-5 20-21 0.0 22-23 0.0 24-25 3.815245951737902E-5 26-27 0.003147577910183769 28-29 1.907622975868951E-5 30-31 0.0 32-33 0.0 34-35 0.012494930491941628 36-37 0.02697378887878697 38-39 0.021422606019008316 40-41 0.008641532080686347 42-43 0.009213818973447033 44-45 0.004387532844498587 46-47 0.005303191872915683 48-49 0.0030331205316316318 50-51 7.058205010715119E-4 52-53 0.0 54-55 0.0 56-57 9.538114879344755E-5 58-59 0.00154517461045385 60-61 0.0024799098686296363 62-63 0.0012590311640735077 64-65 0.0024989860983883256 66-67 0.002098385273455846 68-69 0.004139541857635623 70-71 1.1445737855213704E-4 72-73 0.0 74-75 0.0 76-77 0.09612512175403644 78-79 0.015795118240194913 80-81 3.2429590589772164E-4 82-83 0.0 84-85 0.0 86-87 0.10896342438163449 88-89 0.4189903104198564 90-91 0.4213176104504165 92-93 0.0 94-95 1.907622975868951E-5 96-97 3.815245951737902E-5 98-99 1.907622975868951E-4 100-101 3.2429590589772164E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2621063.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.0852532471828 #Duplication Level Percentage of deduplicated Percentage of total 1 76.65951786295285 41.46149437424739 2 13.368537858235149 14.460815112143974 3 4.380120802016471 7.106998284909428 4 1.879994654566315 4.067199479822764 5 0.9698238768025342 2.6226584991014836 6 0.6078731755016792 1.9726184783506506 7 0.3819969150597147 1.4462279923453072 8 0.2742843007086726 1.1867788692443997 9 0.19929109880540927 0.9700838593919899 >10 1.1072218189312795 10.995971135111827 >50 0.09740536631165032 3.6411058327963266 >100 0.06807056359984619 6.727334185979729 >500 0.00466111601875579 1.7264486732437623 >1k 0.0010593445497135408 0.9488420427871163 >5k 1.4124593996180543E-4 0.6654231805239063 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9372 0.35756485059687615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8049 0.3070891466553837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4962 0.18931250412523468 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.815245951737902E-5 7 3.815245951737902E-5 0.0 0.0 0.0 3.815245951737902E-5 8 3.815245951737902E-5 0.0 0.0 0.0 7.630491903475804E-5 9 3.815245951737902E-5 0.0 0.0 3.815245951737902E-5 7.630491903475804E-5 10-11 3.815245951737902E-5 0.0 0.0 9.538114879344755E-5 7.630491903475804E-5 12-13 3.815245951737902E-5 0.0 0.0 1.7168606782820559E-4 7.630491903475804E-5 14-15 3.815245951737902E-5 0.0 0.0 1.907622975868951E-4 7.630491903475804E-5 16-17 3.815245951737902E-5 0.0 0.0 2.4799098686296365E-4 7.630491903475804E-5 18-19 3.815245951737902E-5 0.0 0.0 2.6706721662165313E-4 1.1445737855213706E-4 20-21 3.815245951737902E-5 0.0 0.0 3.242959058977217E-4 1.1445737855213706E-4 22-23 3.815245951737902E-5 0.0 0.0 5.150582034846167E-4 1.3353360831082657E-4 24-25 3.815245951737902E-5 0.0 0.0 7.248967308302013E-4 1.5260983806951608E-4 26-27 3.815245951737902E-5 0.0 0.0 0.001011040177210544 1.5260983806951608E-4 28-29 3.815245951737902E-5 0.0 0.0 0.0026897483959752205 1.7168606782820559E-4 30-31 3.815245951737902E-5 0.0 0.0 0.008202778796236489 2.098385273455846E-4 32-33 3.815245951737902E-5 0.0 0.0 0.017721817445822553 2.2891475710427411E-4 34-35 3.815245951737902E-5 0.0 0.0 0.032792038955187265 2.6706721662165313E-4 36-37 3.815245951737902E-5 0.0 0.0 0.060490724564804435 2.6706721662165313E-4 38-39 3.815245951737902E-5 0.0 0.0 0.10188614314116068 2.6706721662165313E-4 40-41 3.815245951737902E-5 0.0 0.0 0.1458187002754226 2.6706721662165313E-4 42-43 5.722868927606853E-5 0.0 0.0 0.19137273693917317 2.6706721662165313E-4 44-45 7.630491903475804E-5 0.0 0.0 0.2492881704865545 2.861434463803426E-4 46-47 9.538114879344755E-5 0.0 0.0 0.3204234312567077 3.242959058977217E-4 48-49 1.1445737855213706E-4 0.0 0.0 0.39825444867216087 3.4337213565641117E-4 50-51 1.1445737855213706E-4 0.0 0.0 0.4838494915994007 3.4337213565641117E-4 52-53 1.1445737855213706E-4 0.0 0.0 0.5680328935244976 3.815245951737902E-4 54-55 1.907622975868951E-4 0.0 0.0 0.6649019882391228 4.196770546911692E-4 56-57 1.907622975868951E-4 0.0 0.0 0.784147500460691 4.196770546911692E-4 58-59 1.907622975868951E-4 0.0 0.0 0.9137323292114687 4.387532844498587E-4 60-61 1.907622975868951E-4 0.0 0.0 1.0622598541126251 4.5782951420854823E-4 62-63 1.907622975868951E-4 0.0 0.0 1.2125423921515814 4.5782951420854823E-4 64-65 1.907622975868951E-4 0.0 0.0 1.3729353319626427 4.959819737259272E-4 66-67 1.907622975868951E-4 0.0 0.0 1.5587187335825199 4.959819737259272E-4 68-69 1.907622975868951E-4 0.0 0.0 1.7774467839956536 4.959819737259272E-4 70-71 1.907622975868951E-4 0.0 0.0 2.022805251151918 4.959819737259272E-4 72-73 2.2891475710427411E-4 0.0 0.0 2.266866534684592 5.341344332433063E-4 74-75 2.861434463803426E-4 0.0 0.0 2.518768148648087 5.341344332433063E-4 76-77 3.0521967613903215E-4 0.0 0.0 2.7983112195319224 5.341344332433063E-4 78-79 3.0521967613903215E-4 0.0 0.0 3.1164455032175877 5.341344332433063E-4 80-81 3.815245951737902E-4 0.0 0.0 3.473438066921703 5.341344332433063E-4 82-83 3.815245951737902E-4 0.0 0.0 3.8404265750193716 5.341344332433063E-4 84-85 4.0060082493247973E-4 0.0 0.0 4.21702950291542 5.341344332433063E-4 86-87 4.196770546911692E-4 0.0 0.0 4.613509862220023 5.341344332433063E-4 88-89 4.196770546911692E-4 0.0 0.0 5.047379631851657 5.341344332433063E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7080 0.0 38.76929 1 GTATCAA 10985 0.0 33.35991 1 ATCAACG 13140 0.0 27.66446 3 TCAACGC 13325 0.0 27.280375 4 TATCAAC 13390 0.0 27.147945 2 CAACGCA 13550 0.0 26.687288 5 AACGCAG 13880 0.0 26.052794 6 GTACATG 10800 0.0 25.856663 1 TACATGG 10895 0.0 25.132597 2 ACATGGG 10915 0.0 23.95613 3 TACCTGG 2135 0.0 23.783442 2 ACGCAGA 15760 0.0 22.824528 7 CGCAGAG 15890 0.0 22.548197 8 CATGGGG 5680 0.0 22.223993 4 GAGTACT 8930 0.0 22.081736 12-13 GTACTTT 9355 0.0 20.926365 14-15 GCAGAGT 17345 0.0 20.575035 9 GTGTAGC 1365 0.0 19.550327 1 GTACCTG 2650 0.0 19.241394 1 AGTACTT 9270 0.0 19.01922 12-13 >>END_MODULE