##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939197_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 79182 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.619332676618424 33.0 31.0 34.0 30.0 34.0 2 31.585915991008058 33.0 31.0 34.0 30.0 34.0 3 31.600401606425702 34.0 31.0 34.0 30.0 34.0 4 34.99506200904246 37.0 35.0 37.0 33.0 37.0 5 35.05419160920411 37.0 35.0 37.0 33.0 37.0 6 35.223687201636736 37.0 35.0 37.0 33.0 37.0 7 35.217511555656586 37.0 35.0 37.0 33.0 37.0 8 35.222474804879894 37.0 35.0 37.0 33.0 37.0 9 36.75226692935263 39.0 37.0 39.0 33.0 39.0 10-11 36.69091460180344 39.0 37.0 39.0 33.0 39.0 12-13 36.63866787906342 39.0 37.0 39.0 33.0 39.0 14-15 37.62753529842641 40.0 38.0 41.0 32.5 41.0 16-17 37.59270414993307 40.0 38.0 41.0 32.5 41.0 18-19 37.62083554343159 40.0 37.5 41.0 33.0 41.0 20-21 37.5419729231391 40.0 37.5 41.0 33.0 41.0 22-23 37.42863908464045 40.0 37.0 41.0 33.0 41.0 24-25 37.343335606577256 40.0 37.0 41.0 32.5 41.0 26-27 37.068279406935915 40.0 36.5 41.0 32.5 41.0 28-29 36.87269834053194 40.0 36.0 41.0 32.0 41.0 30-31 36.60222020156096 39.0 35.0 41.0 32.0 41.0 32-33 36.11843600818368 39.0 35.0 41.0 31.0 41.0 34-35 35.60095728827259 38.0 35.0 41.0 30.0 41.0 36-37 35.19786062488949 38.0 35.0 40.5 29.0 41.0 38-39 34.76178298098053 38.0 35.0 40.0 26.0 41.0 40-41 34.56493268672173 38.0 35.0 40.0 24.5 41.0 42-43 34.20418782046424 38.0 35.0 40.0 23.0 41.0 44-45 33.648291278320826 37.0 35.0 40.0 16.5 41.0 46-47 32.935610365992275 37.0 33.5 40.0 4.5 41.0 48-49 32.41078780530929 37.0 33.0 40.0 2.0 41.0 50-51 31.168422116137506 36.0 31.5 39.0 2.0 40.0 52-53 31.253068879290748 35.5 32.5 39.5 2.0 40.0 54-55 31.560089414260815 36.5 33.0 40.0 2.0 41.0 56-57 31.163117880326336 35.5 32.0 40.0 2.0 41.0 58-59 30.8451226288803 36.0 31.0 40.0 2.0 41.0 60-61 30.28705387588088 35.0 31.0 39.5 2.0 41.0 62-63 29.7850205854866 35.0 30.0 39.0 2.0 41.0 64-65 29.291928721174003 35.0 28.0 39.0 2.0 40.5 66-67 28.66789169255639 35.0 27.0 38.0 2.0 39.5 68-69 28.085511858755776 35.0 26.0 37.0 2.0 39.0 70-71 27.41495541916092 35.0 25.0 36.5 2.0 38.0 72-73 26.83429946199894 34.5 23.0 36.0 2.0 37.0 74-75 26.40507943724584 34.0 20.0 35.0 2.0 37.0 76-77 25.944646510570585 34.0 16.5 35.0 2.0 36.5 78-79 25.452495516657827 34.0 9.0 35.0 2.0 36.0 80-81 24.98383470990882 33.0 2.0 35.0 2.0 36.0 82-83 24.557986663635674 33.0 2.0 35.0 2.0 35.0 84-85 24.15985324947589 33.0 2.0 35.0 2.0 35.0 86-87 23.85908413528327 33.0 2.0 35.0 2.0 35.0 88-89 23.33277765148645 32.5 2.0 35.0 2.0 35.0 90-91 22.841763279533225 32.0 2.0 35.0 2.0 35.0 92-93 22.385857896996793 31.0 2.0 35.0 2.0 35.0 94-95 21.87796468894446 31.0 2.0 35.0 2.0 35.0 96-97 21.272258846707587 31.0 2.0 35.0 2.0 35.0 98-99 20.639299335707612 30.5 2.0 34.5 2.0 35.0 100-101 18.616440605188046 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.210815301714959 1101 2 -0.09327720151570773 1101 3 -0.2382029018545424 1101 4 -0.4543619124617564 1101 5 -0.6995843148604166 1101 6 -0.44013811854962626 1101 7 -0.39394910056945065 1101 8 -0.5317449923861801 1101 9 -0.5638131073951129 1101 10-11 -0.2796616306385076 1101 12-13 -0.2421690159113865 1101 14-15 -0.7340787750555009 1101 16-17 -0.5695602760937888 1101 18-19 -0.1453199283769422 1101 20-21 -0.05576055911576816 1101 22-23 -0.16159310557075912 1101 24-25 -0.5047731874143153 1101 26-27 0.22693298607047296 1101 28-29 -0.10772028401164846 1101 30-31 -0.12779088441537567 1101 32-33 0.1749703232126265 1101 34-35 -0.19908278025889814 1101 36-37 0.36988163872742064 1101 38-39 0.6684916148047364 1101 40-41 0.6029654194599416 1101 42-43 0.17198224262259032 1101 44-45 0.640152126589868 1101 46-47 -0.027900648196393263 1101 48-49 0.36121408058432536 1101 50-51 0.28824632790308513 1101 52-53 0.26087646731065917 1101 54-55 -0.6285501087614982 1101 56-57 -0.6142496750839079 1101 58-59 -0.6315085520984347 1101 60-61 -0.12941941789945233 1101 62-63 0.31296502835650486 1101 64-65 -0.2704413088109199 1101 66-67 -0.7825888297630925 1101 68-69 -0.7009912161493368 1101 70-71 -0.8173085195278702 1101 72-73 -0.9241047230784503 1101 74-75 -0.38472012315519066 1101 76-77 0.08117834128971779 1101 78-79 0.48559098416615853 1101 80-81 0.14076142361013666 1101 82-83 0.42731504028795086 1101 84-85 0.6627223258442569 1101 86-87 0.5202656140399142 1101 88-89 -0.4528865672301876 1101 90-91 -0.09353151940332083 1101 92-93 -0.5308106139068407 1101 94-95 0.03971646185558342 1101 96-97 -0.0267797884272305 1101 98-99 0.2944196229454903 1101 100-101 1.2574121914257006 1102 1 0.4210632561598011 1102 2 0.5874628364349554 1102 3 0.39225250611509566 1102 4 0.6955432108779007 1102 5 0.44984642517753315 1102 6 0.8943021091543599 1102 7 0.7611742390889944 1102 8 0.4099058615037592 1102 9 0.5452950519976767 1102 10-11 0.40155279061387006 1102 12-13 0.5272806994586361 1102 14-15 0.5273064241854826 1102 16-17 0.9937698946841991 1102 18-19 0.6487977186818839 1102 20-21 0.6192868792143997 1102 22-23 0.5869837445241188 1102 24-25 0.5844108733067372 1102 26-27 0.9313921891065249 1102 28-29 0.7547085584930926 1102 30-31 0.9514311269698226 1102 32-33 0.8573707027192725 1102 34-35 0.5196079218283884 1102 36-37 -0.05137073318908847 1102 38-39 -0.009164931684821909 1102 40-41 -0.2208011839556221 1102 42-43 -0.41649024314591543 1102 44-45 -0.24291238953916405 1102 46-47 -0.4842573844392817 1102 48-49 -0.7588048947477368 1102 50-51 -0.7753210345257564 1102 52-53 -0.290119737622927 1102 54-55 0.12737968250985077 1102 56-57 0.027353740475867028 1102 58-59 -0.35043644583657496 1102 60-61 0.7970511703358412 1102 62-63 1.344511517920072 1102 64-65 1.5431260536179288 1102 66-67 1.3253333713754252 1102 68-69 1.3533256718962043 1102 70-71 0.8076914804721298 1102 72-73 -0.6513343245964762 1102 74-75 -1.2948244874815629 1102 76-77 -0.9804914879322943 1102 78-79 -0.19325152057767525 1102 80-81 -0.00653459157012648 1102 82-83 -0.5990132329568283 1102 84-85 -0.303122076432782 1102 86-87 -0.1123245187874069 1102 88-89 0.1585935086711423 1102 90-91 -0.27498313230654503 1102 92-93 -0.4762565342104459 1102 94-95 -0.13485659317288068 1102 96-97 -0.6275862400401344 1102 98-99 -0.7663014396731072 1102 100-101 -1.4909274670183201 1103 1 0.3635184991265561 1103 2 0.542066416997617 1103 3 0.8997120116564439 1103 4 0.5706497752427779 1103 5 0.7910655215117188 1103 6 0.8646772157187215 1103 7 0.9220864300523317 1103 8 0.4156603372070862 1103 9 1.2669489309405577 1103 10-11 1.1327974623956223 1103 12-13 1.0688408017604303 1103 14-15 1.2572723235887224 1103 16-17 1.3112251376509576 1103 18-19 1.1445351440868237 1103 20-21 1.2252118579015274 1103 22-23 1.5577851085479892 1103 24-25 1.4769939935113428 1103 26-27 1.526021345116753 1103 28-29 1.115748626694291 1103 30-31 1.386000606935724 1103 32-33 1.2494210010994848 1103 34-35 0.960571263686866 1103 36-37 0.831195336717137 1103 38-39 1.4449058270534536 1103 40-41 1.1582482619096837 1103 42-43 0.2671201575360982 1103 44-45 -0.8425500706985858 1103 46-47 -1.3376248183693704 1103 48-49 -0.9337835818747635 1103 50-51 -1.077111315855678 1103 52-53 -1.0779500871540435 1103 54-55 -1.7714907352224607 1103 56-57 -1.5673819798992419 1103 58-59 -1.3844304782321402 1103 60-61 -0.7236223164501787 1103 62-63 -0.8295080899230634 1103 64-65 -0.6840691722985284 1103 66-67 -0.45439808642508694 1103 68-69 -0.44771865887958384 1103 70-71 -0.7520911282235225 1103 72-73 -0.5500981779639105 1103 74-75 -0.3876420492888961 1103 76-77 -0.925717404385832 1103 78-79 -0.8383921855393126 1103 80-81 -1.829318052780696 1103 82-83 -1.9797677086601553 1103 84-85 -1.500905537643355 1103 86-87 -1.6708283551045433 1103 88-89 -1.6930688954209323 1103 90-91 -1.0340411033210373 1103 92-93 -1.4922411889419003 1103 94-95 -1.4097926545539536 1103 96-97 -1.1040355154024546 1103 98-99 -1.477085753398594 1103 100-101 -2.712901039055831 1104 1 0.253291922274137 1104 2 0.13116623328665256 1104 3 -0.3536049918136541 1104 4 0.03564263092761166 1104 5 -0.2736415615664569 1104 6 0.42764925082792615 1104 7 0.38524217612296496 1104 8 -0.2634578501780922 1104 9 -0.16762955446462513 1104 10-11 0.16025204496349232 1104 12-13 0.09211085687371678 1104 14-15 -0.28110285501915655 1104 16-17 -0.08069158004654042 1104 18-19 -0.20870021006708583 1104 20-21 0.49149069031631143 1104 22-23 -0.12490523642037488 1104 24-25 -0.31326932553335496 1104 26-27 0.2636208552208572 1104 28-29 0.8706986164881201 1104 30-31 0.22545763898308024 1104 32-33 0.18348917827850642 1104 34-35 -0.8567383913447699 1104 36-37 -0.18952317726530765 1104 38-39 -0.9881417336732241 1104 40-41 -0.6086429403764981 1104 42-43 -0.7825838636927216 1104 44-45 -1.3150954881307158 1104 46-47 -1.0004131425171536 1104 48-49 -0.7619117713011754 1104 50-51 -0.8242025589665154 1104 52-53 -0.7983011792136487 1104 54-55 -0.5636932255266025 1104 56-57 -0.8474391344205721 1104 58-59 -0.2664472163419589 1104 60-61 -0.4251940657867763 1104 62-63 -1.029038606354991 1104 64-65 -1.0277107318004646 1104 66-67 -0.6662780619302922 1104 68-69 -0.813099348816305 1104 70-71 -1.1201254209363185 1104 72-73 -0.8843500661043464 1104 74-75 -0.9738841395113909 1104 76-77 -1.03206563871937 1104 78-79 -0.6938728958883615 1104 80-81 -0.4194384855220754 1104 82-83 -1.2443114931059611 1104 84-85 -1.105110477426983 1104 86-87 -0.3603568303035658 1104 88-89 -0.3103377257944757 1104 90-91 0.09649574093289104 1104 92-93 0.4771403174879829 1104 94-95 0.23258334054709096 1104 96-97 -0.9673749724345022 1104 98-99 -0.9935176782812256 1104 100-101 -1.6380898530995118 1105 1 -0.02249795210728678 1105 2 0.19835313850172653 1105 3 0.40695838846803767 1105 4 -0.04372546956724932 1105 5 0.0885586668309557 1105 6 0.282616894527969 1105 7 0.2830343344785007 1105 8 0.17182954989803534 1105 9 -0.05247919283539204 1105 10-11 0.45878649490639845 1105 12-13 0.6922250555794633 1105 14-15 1.0976561857117133 1105 16-17 1.161695172903599 1105 18-19 0.7330584500014368 1105 20-21 0.8740563545721187 1105 22-23 0.6601157000090154 1105 24-25 0.7131867079649368 1105 26-27 0.6702634132718686 1105 28-29 0.4772840751862617 1105 30-31 0.058346866238501605 1105 32-33 0.5980304801437555 1105 34-35 -0.16878635480118476 1105 36-37 -0.2624200177677878 1105 38-39 -0.6985528490139217 1105 40-41 -1.2285515814118995 1105 42-43 -1.07845367716817 1105 44-45 -1.313858335485115 1105 46-47 -0.7044481634535842 1105 48-49 -0.5350370092151451 1105 50-51 -0.42993152737154006 1105 52-53 -0.25852196337809374 1105 54-55 0.044510048169627225 1105 56-57 -0.30670826270378626 1105 58-59 -0.06088954599555407 1105 60-61 0.1813913929113582 1105 62-63 -0.04121980163477801 1105 64-65 -0.045506696779529676 1105 66-67 -0.3004615411841911 1105 68-69 -0.18900342190347885 1105 70-71 -0.43217338439273334 1105 72-73 0.26639222548301333 1105 74-75 0.22643942348823032 1105 76-77 0.4745959524492669 1105 78-79 0.3123128673396529 1105 80-81 0.816001179495057 1105 82-83 1.29109010567592 1105 84-85 1.4957650459837488 1105 86-87 1.5536134780329398 1105 88-89 1.6506443830749582 1105 90-91 1.5189357866123743 1105 92-93 1.6910725595892373 1105 94-95 0.4350957120735437 1105 96-97 0.7136299761760831 1105 98-99 0.8720133457005019 1105 100-101 0.49695011644750053 1106 1 -0.132858312467647 1106 2 -0.4060012158526263 1106 3 0.11529172180136982 1106 4 0.189007263165486 1106 5 0.43840851668080205 1106 6 0.6974066843177553 1106 7 0.4891154155595814 1106 8 0.5569646850331722 1106 9 0.4088571435009456 1106 10-11 -0.12867596755607025 1106 12-13 0.5963166471710508 1106 14-15 0.929675705231233 1106 16-17 1.023181659390083 1106 18-19 0.6046800716230578 1106 20-21 0.6850548530706106 1106 22-23 0.2875556399489554 1106 24-25 0.7420906118688322 1106 26-27 0.43302617603463034 1106 28-29 0.04147326437544763 1106 30-31 0.11033635572799483 1106 32-33 0.5338412909545625 1106 34-35 0.19607851006367838 1106 36-37 -0.1820896874374469 1106 38-39 0.20366186570079492 1106 40-41 0.17666613630581907 1106 42-43 0.41234962613513204 1106 44-45 -0.11791238953916405 1106 46-47 0.661167452162033 1106 48-49 -1.0337231954013326 1106 50-51 -0.5849615574015701 1106 52-53 -1.1573582997144278 1106 54-55 -1.1516235854640051 1106 56-57 -1.7524665209620451 1106 58-59 -2.251580236686248 1106 60-61 -2.0044194178994523 1106 62-63 -1.9545080899230634 1106 64-65 -2.2583445346173683 1106 66-67 -1.9414149946376469 1106 68-69 -2.07845537385543 1106 70-71 -1.803419630638981 1106 72-73 -1.0340957625049754 1106 74-75 -1.4700695855207826 1106 76-77 -0.7427463898930746 1106 78-79 -0.034346291819502994 1106 80-81 0.026962140456017636 1106 82-83 0.16365016573598012 1106 84-85 -0.8043475666288593 1106 86-87 0.04535521977468093 1106 88-89 0.46333207076264316 1106 90-91 -0.10831646563987718 1106 92-93 -0.1139199329032543 1106 94-95 0.9292773937552248 1106 96-97 0.7404693155154227 1106 98-99 -0.5951413089541511 1106 100-101 -1.3973817153843342 1107 1 -0.22520065480999207 1107 2 -0.07191713176854364 1107 3 -0.07952809801845007 1107 4 -0.5842660101077897 1107 5 -0.18171160343931803 1107 6 -0.4606263487152802 1107 7 -0.13588458444041862 1107 8 -0.2876299095614243 1107 9 -0.430857571213771 1107 10-11 -0.35202431590441563 1107 12-13 -0.010477647123238398 1107 14-15 -0.2604519223963919 1107 16-17 -0.6220886108801906 1107 18-19 -0.3074820905391036 1107 20-21 0.00243473295049057 1107 22-23 -0.17772213782882318 1107 24-25 -0.48275923798101417 1107 26-27 -0.5864933434848822 1107 28-29 -0.1037970058948261 1107 30-31 0.07186037975201032 1107 32-33 0.08451696663023966 1107 34-35 0.22310553709071002 1107 36-37 0.6902826849349211 1107 38-39 0.9433390428779731 1107 40-41 0.8187457158853988 1107 42-43 0.6445192958048054 1107 44-45 0.4496551780284008 1107 46-47 0.9644707554653351 1107 48-49 1.2960440718659356 1107 50-51 0.7997982023581862 1107 52-53 0.7009374960813659 1107 54-55 1.0647803184398974 1107 56-57 0.5041025481070278 1107 58-59 0.10127261616660732 1107 60-61 0.6476076091275758 1107 62-63 1.1479693875544115 1107 64-65 1.1977365464637124 1107 66-67 0.9900789993563492 1107 68-69 1.4528884699884124 1107 70-71 1.5272860750667263 1107 72-73 1.232608441699231 1107 74-75 1.179142126190932 1107 76-77 1.7313527092060212 1107 78-79 1.339339894366681 1107 80-81 0.7214065849004605 1107 82-83 1.27081983540565 1107 84-85 1.299819100037805 1107 86-87 1.4117215861410486 1107 88-89 1.346590329020902 1107 90-91 1.593260110936697 1107 92-93 1.7856671541838303 1107 94-95 1.3405011174789472 1107 96-97 0.909575922122027 1107 98-99 1.64904037272753 1107 100-101 0.5239771434745251 1108 1 0.8455367492607486 1108 2 0.887208660690586 1108 3 1.11066209217174 1108 4 0.9050566458815297 1108 5 0.9445813561869798 1108 6 0.8625301411078823 1108 7 0.7159672674114361 1108 8 1.1526436973788492 1108 9 1.1295361558466297 1108 10-11 1.0981758842957845 1108 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70-71 -1.3074075294288612 2316 72-73 -1.4977233752662507 2316 74-75 -1.6299293214943802 2316 76-77 -1.7159312083749256 2316 78-79 -1.4901356207523975 2316 80-81 -1.6251280685648872 2316 82-83 -1.1383987891595098 2316 84-85 -1.1977056524374419 2316 86-87 -1.4312674424915421 2316 88-89 -1.185320294473879 2316 90-91 -1.4866167956728802 2316 92-93 -1.9653363245424202 2316 94-95 -2.521286412625411 2316 96-97 -1.8243684182579578 2316 98-99 -1.9044784926725242 2316 100-101 -1.7476979970124944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1222.0 3 180.0 4 167.0 5 170.0 6 153.0 7 165.0 8 183.0 9 243.0 10 251.0 11 354.0 12 582.0 13 796.0 14 903.0 15 1058.0 16 1319.0 17 1734.0 18 1696.0 19 1088.0 20 1248.0 21 1273.0 22 1143.0 23 1155.0 24 1102.0 25 1187.0 26 1235.0 27 1285.0 28 1453.0 29 1607.0 30 1815.0 31 2179.0 32 2646.0 33 3316.0 34 4754.0 35 8676.0 36 9972.0 37 12611.0 38 7749.0 39 511.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.27228280832414 19.224582335879216 13.816164456435004 13.686970399361629 2 14.703242525756904 26.854181151633906 33.35819480437393 25.084381518235254 3 29.17725279031259 28.89411348324548 29.624714016659716 12.30391970978221 4 12.360786296694268 18.448897035585617 38.03805069211807 31.15226597560205 5 10.339857381277499 42.73883578617306 31.34324584028726 15.578060992262174 6 24.532093164326607 36.49357378457961 15.29401357277357 23.68031947832021 7 20.04853203240524 40.20954716074972 21.222653337209156 18.519267469635885 8 23.941205464909064 25.421179682266846 19.45704788746635 31.180566965357738 9 21.759405527682645 24.285660124606657 25.493813898823444 28.46112044888726 10-11 24.795586952950245 26.062505528946406 22.592854705607298 26.54905281249605 12-13 32.9480828046407 26.014205191719537 23.64267118267068 17.395040820969086 14-15 26.26696661021662 27.217981447311885 31.49091322700503 15.02413871546647 16-17 16.521129252388413 31.33498458272254 31.36847293130465 20.77541323358439 18-19 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32.030482276787694 30.101248327849312 21.80674692989964 44-45 18.16700481866599 28.987446107025107 30.813466903373065 22.032082170935833 46-47 20.637920101458466 30.880786303107165 30.625237793278377 17.856055802155993 48-49 19.396510720876694 29.291045959298085 31.737092774744262 19.57535054508095 50-51 15.802657027806843 31.054250293287676 35.21164272805098 17.931449950854496 52-53 17.697044256878872 32.77380733450438 29.708357377943216 19.820791030673522 54-55 15.067398775028847 28.638455978391814 32.535284495111526 23.758860751467807 56-57 19.846094359117387 28.53529751076008 29.152061055153748 22.466547074968783 58-59 22.134201987426486 27.4956905293044 29.52747921314135 20.842628270127765 60-61 18.404597031189613 27.98639136852909 33.94111796047922 19.667893639802077 62-63 18.231154504533063 28.143029227160337 32.54675711659164 21.079059151714958 64-65 17.092071303101598 31.394545097119085 31.454788735073848 20.058594864705473 66-67 17.981910899127257 27.261771869291657 29.758854272376702 24.997462959204384 68-69 15.903886531300945 28.277100103395558 32.04120598552463 23.777807379778874 70-71 20.53279208423189 27.541545097044274 29.00545477610047 22.920208042623365 72-73 18.21704688877473 24.25113051861154 34.311319139220274 23.22050345339346 74-75 16.31624012938826 30.04852059746932 30.960580978657283 22.67465829448514 76-77 18.415929484130757 24.48841117346777 31.45756048067472 25.63809886172675 78-79 18.13536865994282 25.190970351258663 33.856111368203514 22.817549620595003 80-81 19.864463941576226 25.632036717719846 31.667765493457757 22.83573384724617 82-83 17.42502060744404 24.94895694629383 34.35609663306068 23.269925813201446 84-85 17.90594307381129 25.844413586704963 32.26366881614071 23.985974523343035 86-87 18.001394965442902 26.525267896772558 31.965633124088516 23.507704013696024 88-89 18.37592745259687 25.18105143002093 29.945462616526097 26.497558500856112 90-91 18.72431115847161 22.544019891409146 32.23283604810474 26.498832902014513 92-93 20.259145568014613 25.4503018925364 31.59470292759653 22.695849611852452 94-95 18.79415980617254 24.5290677761851 31.71577892506945 24.960993492572907 96-97 15.782830685313797 24.369390796942884 33.47286969206863 26.37490882567469 98-99 19.15083869740305 25.175508133303737 29.23296445445033 26.44068871484288 100-101 18.782894319666205 22.31423824681995 31.67936995092009 27.22349748259375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1896.0 1 1140.0 2 433.0 3 455.5 4 311.5 5 249.0 6 444.0 7 766.5 8 1043.5 9 1008.0 10 709.0 11 456.0 12 354.0 13 319.5 14 313.5 15 330.0 16 359.5 17 371.0 18 353.5 19 367.5 20 409.0 21 451.0 22 490.0 23 531.5 24 583.5 25 643.0 26 678.0 27 739.5 28 847.5 29 907.0 30 966.5 31 997.5 32 927.0 33 888.0 34 873.0 35 788.0 36 704.0 37 630.5 38 561.5 39 516.0 40 459.5 41 444.5 42 444.0 43 431.5 44 428.5 45 560.0 46 1679.0 47 4910.5 48 12310.5 49 12637.5 50 5690.5 51 2704.5 52 1489.5 53 936.5 54 643.5 55 520.5 56 488.0 57 482.0 58 492.0 59 550.5 60 570.0 61 541.0 62 527.0 63 509.0 64 497.0 65 501.5 66 494.5 67 439.0 68 365.0 69 319.0 70 293.5 71 239.5 72 180.5 73 144.0 74 107.5 75 74.5 76 60.5 77 46.5 78 28.0 79 22.5 80 15.5 81 10.0 82 9.5 83 6.5 84 5.5 85 2.5 86 0.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2917329696143063 2 0.09724432320476877 3 0.0871410168977798 4 0.09724432320476877 5 0.11366219595362581 6 0.06693440428380187 7 0.0745118840140436 8 0.0745118840140436 9 0.06693440428380187 10-11 0.06693440428380187 12-13 0.07072314414892274 14-15 0.07072314414892274 16-17 0.06314566441868101 18-19 0.0751433406582304 20-21 0.08208936374428533 22-23 0.08777247354196661 24-25 0.10103306306988961 26-27 0.10103306306988961 28-29 0.09787577984895557 30-31 0.086509560253593 32-33 0.08082645045591169 34-35 0.2822611199515041 36-37 0.42244449496097597 38-39 0.3927660326841959 40-41 0.3649819403399763 42-43 0.3997120557702508 44-45 0.4066580788563057 46-47 0.41928721174004197 48-49 0.4300219746912177 50-51 0.42244449496097597 52-53 0.4237074082493496 54-55 0.4079209921446793 56-57 0.3820312697330201 58-59 0.36119320047485537 60-61 0.3302518249097017 62-63 0.4003435124144376 64-65 0.4237074082493496 66-67 0.4420196509307671 68-69 0.4527544138819429 70-71 0.44454547750751433 72-73 0.4375994544214595 74-75 0.44138819428658027 76-77 0.42244449496097597 78-79 0.38960874946326185 80-81 0.3915031193958223 82-83 0.41297264529817385 84-85 0.4110782753656134 86-87 0.41297264529817385 88-89 0.42560177818191 90-91 0.4470713040842616 92-93 0.43696799777727263 94-95 0.4407567376423935 96-97 0.44138819428658027 98-99 0.4287590614028441 100-101 0.41802429845166833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 79182.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.41633199464525 #Duplication Level Percentage of deduplicated Percentage of total 1 88.3518412259088 33.05801823646789 2 5.802139939919668 4.341895885428507 3 2.0656833293954837 2.318708797453967 4 1.0328416646977419 1.5458058649693112 5 0.5704256252742431 1.0671617286757091 6 0.37128295136193334 0.8335227703265894 7 0.320653457994397 0.8398373367684574 8 0.17551557700745943 0.525371927963426 9 0.1586390792182806 0.5342123209820413 >10 0.837074290343268 5.882650097244324 >50 0.1046342862929085 2.660958298603218 >100 0.13163668275559456 12.406860144982446 >500 0.04725419380970061 14.1421030032078 >1k 0.03037769602052182 19.842893586926323 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3301 4.168876764921321 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2293 2.895860170240711 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2227 2.812507893208052 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1810 2.2858730519562527 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1569 1.9815109494582102 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1361 1.7188249854764974 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1142 1.4422469753226743 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1005 1.2692278548154885 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1004 1.2679649415271146 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 965 1.2187113232805435 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 941 1.1884014043595765 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 1.1820868379177085 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 927 1.170720618322346 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 881 1.1126266070571595 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 880 1.1113636937687859 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 832 1.050743855926852 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 826 1.0431663761966103 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 770 0.9724432320476876 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 761 0.961077012452325 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 651 0.8221565507312268 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 633 0.7994241115405016 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 628 0.7931095450986336 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 567 0.7160718345078426 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 486 0.6137758581495795 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 480 0.6061983784193377 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 477 0.6024096385542169 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 466 0.588517592382107 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 430 0.5430527140006567 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 414 0.5228461013866789 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 372 0.46980374327498675 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 360 0.45464878381450324 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 338 0.4268646914702836 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 321 0.4053951655679321 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 312 0.39402894597256954 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 306 0.3864514662423278 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 304 0.3839256396655806 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 296 0.3738223333585916 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 273 0.34477532772599834 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 266 0.335934934707383 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 253 0.3195170619585259 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 242 0.3056250157864161 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 236 0.2980475360561744 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 220 0.27784092344219646 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 214 0.2702634437119547 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 198 0.25005683109797683 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 194 0.24500517794448234 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 184 0.23237604506074613 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.22606147861887801 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 178 0.22479856533050444 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 168 0.21216943244676822 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 164 0.20711777929327374 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 161 0.20332903942815286 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.19575155969791114 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 148 0.1869111666792958 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 147 0.18564825339092217 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 147 0.18564825339092217 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 136 0.17175620721881235 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 134 0.1692303806420651 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 134 0.1692303806420651 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 117 0.14776085473971357 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 111 0.14018337500947184 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 103 0.1300800687024829 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 96 0.12123967568386754 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 91 0.11492510924199945 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 87 0.10987345608850496 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 84 0.10608471622338411 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 82 0.10355888964663687 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.10229597635826324 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 81 0.10229597635826324 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 6.314566441868101E-4 0.0 6.314566441868101E-4 0.0 20-21 0.0 0.0012629132883736202 0.0 0.0025258265767472404 0.0 22-23 0.0 0.0012629132883736202 0.0 0.010103306306988963 0.0 24-25 0.0 0.0012629132883736202 0.0 0.022100982546538354 0.0 26-27 0.0 0.0012629132883736202 0.0 0.04609633502563714 0.0 28-29 0.0 0.0012629132883736202 0.0 0.13197443863504332 0.0 30-31 0.0 0.0012629132883736202 0.0 0.28920714303755907 0.0 32-33 0.0 0.0012629132883736202 0.0 0.5367381475587886 0.0 34-35 0.0 0.0012629132883736202 0.0 0.8619383193149959 0.0 36-37 0.0 0.0012629132883736202 0.0 1.2730165946806093 0.0 38-39 0.0 0.0012629132883736202 0.0 1.8072289156626506 0.0 40-41 0.0 0.0012629132883736202 0.0 2.3749084387865933 0.0 42-43 0.0 0.0012629132883736202 0.0 2.888914147154656 0.0 44-45 0.0 0.0012629132883736202 0.0 3.4016569422343466 0.0 46-47 0.0 0.0012629132883736202 0.0 4.009749690586244 0.0 48-49 0.0 0.0012629132883736202 0.0 4.531964335328737 0.0 50-51 0.0 0.0012629132883736202 0.0 5.061125003157283 0.0 52-53 0.0 0.0012629132883736202 0.0 5.533454573009017 0.0 54-55 0.0 0.0012629132883736202 0.0 6.014624535879367 0.0 56-57 0.0 0.0012629132883736202 0.0 6.608193781414968 0.0 58-59 0.0 0.0012629132883736202 0.0 7.144931928973756 0.0 60-61 0.0 0.0012629132883736202 0.0 7.816170341744336 0.0 62-63 0.0 0.0012629132883736202 0.0 8.438155136268342 0.0 64-65 0.0 0.0012629132883736202 0.0 8.927534035513121 0.0 66-67 0.0 0.0012629132883736202 0.0 9.544467176883636 0.0 68-69 0.0 0.0012629132883736202 0.0 10.302846606551995 0.0 70-71 0.0 0.0012629132883736202 0.0 11.088378671920385 0.0 72-73 0.0 0.0012629132883736202 0.0 11.823394205753832 0.0 74-75 0.0 0.0012629132883736202 0.0 12.709959334192114 0.0 76-77 0.0 0.0012629132883736202 0.0 13.403298729509231 0.0 78-79 0.0 0.0012629132883736202 0.0 14.44709656235003 0.0 80-81 0.0 0.0012629132883736202 0.0 15.413856684600034 0.0 82-83 0.0 0.0012629132883736202 0.0 16.218963905938217 0.0 84-85 0.0 0.0012629132883736202 0.0 17.252658432472025 0.0 86-87 0.0 0.0012629132883736202 0.0 18.281301305852338 0.0 88-89 0.0 0.0012629132883736202 0.0 19.28089717360006 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGCG 35 0.008311678 27.181751 84-85 CCCGACA 35 0.008311678 27.181751 92-93 CATGGGG 195 5.347829E-10 26.71431 4 GTACTTT 985 0.0 23.783728 14-15 GAGTACT 1060 0.0 22.993883 12-13 GAGAAAA 65 2.9989865E-4 21.84333 12-13 AGTACTC 110 0.0029042577 21.525995 5 ACTTTTT 1135 0.0 21.057484 16-17 ATGGGGG 90 0.0026693372 21.047638 5 AGTACTT 965 0.0 20.843592 12-13 TACTTTT 1010 0.0 19.680624 14-15 CAGAGAA 85 0.0018222525 16.703722 10-11 GTACATG 780 0.0 15.226802 1 TACATGG 785 0.0 15.081906 2 ACATGGG 760 0.0 14.954901 3 GTGGTCG 105 0.0071868235 13.590876 94-95 CTTTTTT 1785 0.0 11.533524 16-17 AGTACAT 670 1.1459633E-10 11.302021 7 CATGGGA 465 1.3298975E-4 10.184341 4 TGTAGAT 375 2.3141183E-8 10.147855 88-89 >>END_MODULE