##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939196_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 77517 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18420475508598 33.0 31.0 34.0 31.0 34.0 2 32.349755537495 34.0 31.0 34.0 30.0 34.0 3 32.41961118206329 34.0 31.0 34.0 30.0 34.0 4 35.90776216829857 37.0 35.0 37.0 35.0 37.0 5 35.90420165899093 37.0 35.0 37.0 35.0 37.0 6 36.032418695253945 37.0 35.0 37.0 35.0 37.0 7 35.99898086871267 37.0 35.0 37.0 35.0 37.0 8 36.04102325941406 37.0 35.0 37.0 35.0 37.0 9 37.612730110814404 39.0 37.0 39.0 35.0 39.0 10-11 37.559445024962265 39.0 37.0 39.0 35.0 39.0 12-13 37.49440122811771 39.0 37.0 39.0 35.0 39.0 14-15 38.498477753267025 40.0 38.0 41.0 34.0 41.0 16-17 38.67390378884632 40.0 38.0 41.0 34.5 41.0 18-19 38.773836706786895 40.0 38.0 41.0 35.0 41.0 20-21 38.75575680173381 40.0 38.0 41.0 35.0 41.0 22-23 38.667498742211386 40.0 38.0 41.0 35.0 41.0 24-25 38.44360591870171 40.0 37.5 41.0 34.0 41.0 26-27 38.08302694892733 40.0 36.5 41.0 34.0 41.0 28-29 37.83760336442329 40.0 36.0 41.0 33.0 41.0 30-31 37.54711869654399 39.0 35.5 41.0 33.0 41.0 32-33 37.083459112194745 39.0 35.0 41.0 33.0 41.0 34-35 36.727750041926285 39.0 35.0 41.0 33.0 41.0 36-37 36.34240876194899 38.5 35.0 41.0 31.5 41.0 38-39 35.957822155140164 38.0 35.0 40.5 30.5 41.0 40-41 35.748519679554164 38.0 35.0 40.0 30.5 41.0 42-43 35.35442548086226 38.0 35.0 40.0 29.5 41.0 44-45 34.850890772346716 38.0 35.0 40.0 26.0 41.0 46-47 34.22688571539146 38.0 35.0 40.0 23.5 41.0 48-49 33.55698749951624 37.0 34.0 40.0 17.5 41.0 50-51 33.04423545802857 37.0 33.0 40.0 5.0 41.0 52-53 32.66836951894423 37.0 33.0 40.0 2.0 41.0 54-55 32.36836435878581 36.5 33.0 40.0 2.0 41.0 56-57 31.950572132564467 36.0 32.5 40.0 2.0 41.0 58-59 31.438813421572043 35.5 32.0 40.0 2.0 41.0 60-61 30.68486267528413 35.0 31.0 39.0 2.0 40.5 62-63 30.11715494665686 35.0 30.0 39.0 2.0 40.0 64-65 29.452158881277654 35.0 29.0 38.0 2.0 40.0 66-67 28.73739953816582 35.0 28.0 37.0 2.0 39.0 68-69 28.07195195892514 35.0 26.5 37.0 2.0 39.0 70-71 27.328631138975968 34.0 25.5 36.0 2.0 38.0 72-73 26.651721557851825 34.0 24.0 35.0 2.0 37.0 74-75 26.23881213153244 33.0 22.0 35.0 2.0 37.0 76-77 24.469677619102907 30.5 20.0 34.0 2.0 35.0 78-79 25.305842589367494 32.5 20.0 34.5 2.0 35.5 80-81 25.3039784821394 33.0 18.0 35.0 2.0 35.0 82-83 24.978953003857217 33.0 11.5 35.0 2.0 35.0 84-85 24.597139982197454 32.5 4.5 35.0 2.0 35.0 86-87 24.11416205477508 32.0 2.0 35.0 2.0 35.0 88-89 23.44352206612743 31.5 2.0 34.0 2.0 35.0 90-91 22.88746984532425 31.0 2.0 34.0 2.0 35.0 92-93 22.540120231691112 31.0 2.0 34.0 2.0 35.0 94-95 22.10142291368345 31.0 2.0 34.0 2.0 35.0 96-97 21.27433982223254 30.0 2.0 34.0 2.0 35.0 98-99 20.343350490860068 29.0 2.0 34.0 2.0 35.0 100-101 15.555491053575345 13.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.741326378128889 1101 2 0.6079292830394181 1101 3 0.6554501734727793 1101 4 0.46452077508096323 1101 5 0.48008395982954966 1101 6 0.42162472369989956 1101 7 0.4137091280465697 1101 8 0.6185113858970581 1101 9 0.8415854380804362 1101 10-11 0.4700055671980081 1101 12-13 0.3538985019217691 1101 14-15 0.7736826327732942 1101 16-17 0.6689391426347129 1101 18-19 0.9431172209821739 1101 20-21 1.0370714454485537 1101 22-23 0.928037900566494 1101 24-25 0.5185539406889319 1101 26-27 0.39807247679439683 1101 28-29 0.7024803045838368 1101 30-31 1.1042391954323634 1101 32-33 1.3089296923063003 1101 34-35 0.9900607479097587 1101 36-37 1.554963909439273 1101 38-39 1.8044623130291768 1101 40-41 1.739626553895448 1101 42-43 2.287049049255181 1101 44-45 1.902305955097738 1101 46-47 1.8241352504700998 1101 48-49 1.781003323476689 1101 50-51 1.8446950255007337 1101 52-53 2.114318172345662 1101 54-55 2.0905757373279528 1101 56-57 2.351200295051246 1101 58-59 2.7915543390421256 1101 60-61 2.0595920840306654 1101 62-63 2.911248796739212 1101 64-65 2.8684651205063894 1101 66-67 3.226707070853795 1101 68-69 3.4807309093730474 1101 70-71 3.6316570787286224 1101 72-73 3.1570633936562835 1101 74-75 3.700286361286782 1101 76-77 3.5494173233495125 1101 78-79 3.032978710487729 1101 80-81 2.5947918151619014 1101 82-83 2.57918484851589 1101 84-85 2.391792826870997 1101 86-87 2.358471862147738 1101 88-89 1.6558360154808867 1101 90-91 1.8624777449920664 1101 92-93 1.9521511154705529 1101 94-95 1.8352156557482893 1101 96-97 1.7261525144318561 1101 98-99 2.9374899778594994 1101 100-101 1.2000743125370494 1102 1 0.294027649315332 1102 2 0.14765385931060138 1102 3 -0.13453923330688156 1102 4 -0.26641142830887077 1102 5 0.05688480728717593 1102 6 0.27093616437786494 1102 7 0.003486670419448501 1102 8 -0.05601191918768933 1102 9 -0.018319222936511892 1102 10-11 -0.0012603226324969796 1102 12-13 0.07476714598956136 1102 14-15 0.08115085311227688 1102 16-17 -0.1297896709246089 1102 18-19 -0.2703361688483312 1102 20-21 0.11771233527905878 1102 22-23 -0.23403837061994182 1102 24-25 0.26312491526520887 1102 26-27 0.2495025615401616 1102 28-29 0.6406423808550201 1102 30-31 -0.01281588931339428 1102 32-33 -0.7354294178631946 1102 34-35 -0.4091712436156669 1102 36-37 -0.7449566414081872 1102 38-39 -1.0954317547674322 1102 40-41 -0.7909613698333686 1102 42-43 -1.960435060914314 1102 44-45 -1.7681124771056531 1102 46-47 -1.506902885123118 1102 48-49 -1.6911894731334804 1102 50-51 -1.266931033821301 1102 52-53 -0.08483437002722383 1102 54-55 0.04621662715845787 1102 56-57 0.5028157611529416 1102 58-59 -0.29544248299177056 1102 60-61 0.24179547386117406 1102 62-63 -2.088089126989601 1102 64-65 -1.254283820171576 1102 66-67 -0.2570058528750181 1102 68-69 -0.25377650588118783 1102 70-71 0.3937068668642141 1102 72-73 0.18937271569017966 1102 74-75 0.007886996879999941 1102 76-77 0.47129232334951254 1102 78-79 0.8118452359114592 1102 80-81 0.8488966880432578 1102 82-83 -0.17293379555190924 1102 84-85 -0.1733554782137503 1102 86-87 -0.6506647903946359 1102 88-89 -1.2326703404513175 1102 90-91 -1.1000487380587813 1102 92-93 -0.9635003675802949 1102 94-95 -1.5317070137432367 1102 96-97 -1.155071002517296 1102 98-99 -0.6553331153608397 1102 100-101 -0.9670337383104091 1103 1 0.6194513781288862 1103 2 0.13148697534710863 1103 3 0.5927097888573982 1103 4 0.2587515443117354 1103 5 0.31998780598339494 1103 6 0.2766728006229755 1103 7 0.2697187434311843 1103 8 0.03942484743551944 1103 9 0.1423065919265909 1103 10-11 0.5386353748903119 1103 12-13 0.3399561942294653 1103 14-15 0.20709609431175835 1103 16-17 0.7093237580193303 1103 18-19 0.6618672209821739 1103 20-21 0.5393550992947098 1103 22-23 0.7328455928741846 1103 24-25 0.8403087483812399 1103 26-27 0.8127359383328638 1103 28-29 0.3629370353530632 1103 30-31 0.5854891954323662 1103 32-33 0.7862133461524579 1103 34-35 0.7317674786789894 1103 36-37 0.9940264094392717 1103 38-39 1.2155200053368702 1103 40-41 1.162102515433908 1103 42-43 1.3073615492551838 1103 44-45 1.6978588397131276 1103 46-47 1.8919237120085626 1103 48-49 1.9990321696305315 1103 50-51 2.1388056024238082 1103 52-53 2.325856633884122 1103 54-55 2.598628621943334 1103 56-57 2.0159839488974036 1103 58-59 2.433501454426743 1103 60-61 2.786515160953744 1103 62-63 2.480359373662292 1103 64-65 3.0707487743525412 1103 66-67 3.1499041862384125 1103 68-69 3.4094568709115087 1103 70-71 3.408700347959389 1103 72-73 4.1065826244255135 1103 74-75 4.132137322825244 1103 76-77 3.84821540027259 1103 78-79 3.8115844797184977 1103 80-81 4.034815853623439 1103 82-83 4.292646386977427 1103 84-85 4.437465903794074 1103 86-87 4.220130515993894 1103 88-89 4.940211015480887 1103 90-91 4.781828706530529 1103 92-93 5.3337616923936295 1103 94-95 4.595672386517517 1103 96-97 4.332282322124161 1103 98-99 4.709725554782576 1103 100-101 2.971949312537051 1104 1 0.15747954714297663 1104 2 -0.05096156203100577 1104 3 -0.3240832772314448 1104 4 -0.14991584463734853 1104 5 -0.13743364580425066 1104 6 0.013834230742155285 1104 7 0.054334128046569674 1104 8 -0.13478967044097345 1104 9 0.21944635357339592 1104 10-11 0.12108391226842485 1104 12-13 0.45469075544289694 1104 14-15 0.2976703088296304 1104 16-17 0.22351660742344848 1104 18-19 0.337923559010342 1104 20-21 0.3545230299555939 1104 22-23 0.21941114000311757 1104 24-25 0.1650108068861158 1104 26-27 0.23852142045636526 1104 28-29 -0.2621983925992666 1104 30-31 -0.32577841020144405 1104 32-33 -0.01974548375003593 1104 34-35 0.13057571269849433 1104 36-37 -0.18766373140579873 1104 38-39 -0.44861867288631174 1104 40-41 -0.6445063686397674 1104 42-43 -0.8363004225758033 1104 44-45 -1.5135171082825494 1104 46-47 -1.9315865805158197 1104 48-49 -1.9696568877909186 1104 50-51 -2.1085400097105342 1104 52-53 -2.1215973206120857 1104 54-55 -2.2451416922495113 1104 56-57 -1.9871579795966419 1104 58-59 -1.6227282313804103 1104 60-61 -1.4861825638566586 1104 62-63 -0.7021975060776917 1104 64-65 -0.9690128724513549 1104 66-67 -1.4978747953433889 1104 68-69 -0.7436308864016006 1104 70-71 -0.7267276043699695 1104 72-73 -0.7157359021183645 1104 74-75 -0.729401138713218 1104 76-77 -0.6061724653828797 1104 78-79 -0.2590547402164951 1104 80-81 -0.5923780792042983 1104 82-83 -1.2007447289489015 1104 84-85 -0.449316328058579 1104 86-87 -0.24639169419029017 1104 88-89 -0.46542278733601705 1104 90-91 0.07803672386530636 1104 92-93 -0.13752758171254698 1104 94-95 -0.07312501326579479 1104 96-97 -0.05850857711743984 1104 98-99 -0.2263524517179647 1104 100-101 -1.3745295607023866 1105 1 0.16230852098603066 1105 2 0.35346499732513337 1105 3 0.12018231632992382 1105 4 0.18952077508096465 1105 5 -0.11188032588473362 1105 6 0.15689258084275792 1105 7 -0.1313801576677136 1105 8 -0.23639932838865718 1105 9 -0.16868241906242076 1105 10-11 -0.18334264708770576 1105 12-13 -0.023780069506806 1105 14-15 -0.1687280815124197 1105 16-17 0.015367714063287963 1105 18-19 -0.3095613504464012 1105 20-21 -0.007348197408589385 1105 22-23 0.10482361485220792 1105 24-25 0.2611878692603611 1105 26-27 0.008340333937255195 1105 28-29 0.37502494744098414 1105 30-31 0.20691776686093277 1105 32-33 0.6323671923062975 1105 34-35 0.15814110505261425 1105 36-37 -0.19883073341787139 1105 38-39 0.1732123130291754 1105 40-41 0.029135482466870144 1105 42-43 -0.22835273645910803 1105 44-45 0.05610059795488098 1105 46-47 0.36609953618438595 1105 48-49 0.5814497520481225 1105 50-51 0.23331109692930596 1105 52-53 0.057175315202805166 1105 54-55 0.1711560944708097 1105 56-57 -0.5235764906630358 1105 58-59 -0.7818831609578751 1105 60-61 0.3453063697449501 1105 62-63 -1.0751351318322158 1105 64-65 -0.16881166520789748 1105 66-67 0.5273767137109395 1105 68-69 -0.48065301919838177 1105 70-71 -1.2176732784142352 1105 72-73 -1.0741866063437193 1105 74-75 -1.4008297101417888 1105 76-77 -0.8572791052219166 1105 78-79 -1.1719320037979841 1105 80-81 -0.8536456848380993 1105 82-83 -0.4172437229126835 1105 84-85 -1.4180286017004313 1105 86-87 -1.805144209280833 1105 88-89 -1.7455032702333995 1105 90-91 -2.643995469293646 1105 92-93 -2.2503042416723034 1105 94-95 -1.2779539871088552 1105 96-97 -0.4611242712824293 1105 98-99 0.2328024778595008 1105 100-101 -0.1494792588915228 1106 1 -0.39676483808732854 1106 2 -0.2662261223659854 1106 3 -0.2064586103110031 1106 4 0.048980234540422884 1106 5 -0.25049036449477313 1106 6 -0.7299800060298267 1106 7 0.2570368307492714 1106 8 -0.1302217222110471 1106 9 -0.6115395619195638 1106 10-11 -0.1636515273965884 1106 12-13 -0.26181095753769057 1106 14-15 -0.35173966452400407 1106 16-17 -0.30065545195988364 1106 18-19 -0.27732196820701915 1106 20-21 0.3882116481512625 1106 22-23 0.3621595221881151 1106 24-25 0.6353191433916408 1106 26-27 0.9728190984160179 1106 28-29 0.40243807485411054 1106 30-31 0.1692729792161458 1106 32-33 -0.45141659147748214 1106 34-35 -0.2381909412794343 1106 36-37 -0.11543305002018656 1106 38-39 0.14483393465079786 1106 40-41 0.3019154052468025 1106 42-43 -0.4061519642583349 1106 44-45 0.7686488605031414 1106 46-47 0.2979528180376647 1106 48-49 -0.3984730278746653 1106 50-51 -0.8556850420668312 1106 52-53 -0.6086548006273134 1106 54-55 -1.0481489248342086 1106 56-57 -1.1000243671109153 1106 58-59 -1.2048561339308463 1106 60-61 -1.285002510563931 1106 62-63 -1.7925096492067318 1106 64-65 -2.050664947061179 1106 66-67 -1.5289939426597172 1106 68-69 -1.4561356446810088 1106 70-71 -0.8470169077578653 1106 72-73 -0.6917541739112849 1106 74-75 -0.3712930306051092 1106 76-77 -1.5692482171910278 1106 78-79 -1.3238625057284885 1106 80-81 -0.37121325240566705 1106 82-83 -0.3093286649976221 1106 84-85 -0.5178355515073818 1106 86-87 -0.7553372594738832 1106 88-89 -1.1030322277623554 1106 90-91 -0.21079083608901428 1106 92-93 -0.13505327642133835 1106 94-95 -0.04590765506252126 1106 96-97 -0.4835180936762491 1106 98-99 0.0584781535351766 1106 100-101 0.4800574206451582 1107 1 0.37017601581004556 1107 2 0.2043966743437693 1107 3 -0.1473125076866424 1107 4 0.031549760588212905 1107 5 -0.4623979242284193 1107 6 -0.23213433427111596 1107 7 0.3586819541335231 1107 8 0.4996255163318395 1107 9 0.4464314525731865 1107 10-11 0.31500103821250036 1107 12-13 -0.08858338213619987 1107 14-15 0.29338371972981747 1107 16-17 -0.039394190698615716 1107 18-19 -0.17871248916275562 1107 20-21 0.27246546718768627 1107 22-23 0.11734949476939249 1107 24-25 -0.17607921148498207 1107 26-27 -0.25025179856791624 1107 28-29 -0.2736292968654439 1107 30-31 -0.43335138427777764 1107 32-33 -0.11690817001254317 1107 34-35 0.5633170884894696 1107 36-37 -1.7648911145329294E-4 1107 38-39 0.24060361737700475 1107 40-41 0.4904191263592139 1107 42-43 0.25953546229866475 1107 44-45 -0.1258869796848714 1107 46-47 0.012993946122271893 1107 48-49 -0.49639703884215436 1107 50-51 -0.8352189237746259 1107 52-53 -0.2226383493934705 1107 54-55 0.3314045416757807 1107 56-57 0.22880446171791036 1107 58-59 0.2427545202015473 1107 60-61 0.19365005504515764 1107 62-63 -0.08207094963760042 1107 64-65 0.5603808813759521 1107 66-67 0.6330702954914749 1107 68-69 0.4276285543005862 1107 70-71 1.2699043613373178 1107 72-73 1.2743641182939633 1107 74-75 -0.012009834365390049 1107 76-77 0.9060749320451649 1107 78-79 0.9032232757051197 1107 80-81 0.7688180832778464 1107 82-83 0.5918660079361793 1107 84-85 0.331557319624622 1107 86-87 -0.887338826258059 1107 88-89 0.0677472473649452 1107 90-91 0.5913047377456913 1107 92-93 0.5901719488038886 1107 94-95 1.3156277934294458 1107 96-97 1.0014016086347546 1107 98-99 1.89367204307689 1107 100-101 -0.019355035289036593 1108 1 0.26945137812889186 1108 2 0.3641792830394195 1108 3 -0.08517482652722208 1108 4 0.039520775080966075 1108 5 -0.07616604017044892 1108 6 -0.6002502763001019 1108 7 -0.13316587195343033 1108 8 0.10288638589705812 1108 9 -0.18653956191956667 1108 10-11 -0.18155693280198903 1108 12-13 -0.3648514980782309 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-1.529912596885108 2316 54-55 -1.5927175319028137 2316 56-57 -1.1061314357179874 2316 58-59 -1.7174600840347942 2316 60-61 -1.0654079159693346 2316 62-63 -1.3369483186454012 2316 64-65 -1.8773281487243771 2316 66-67 -1.3375958137615918 2316 68-69 -1.1665046675500292 2316 70-71 -1.5143765751175309 2316 72-73 -1.580917375574483 2316 74-75 -1.3447857540978312 2316 76-77 -1.4565922920351007 2316 78-79 -1.3047616741276578 2316 80-81 -1.1113379925304052 2316 82-83 -1.1083151514841099 2316 84-85 -1.1971494808213095 2316 86-87 -1.9846771763138022 2316 88-89 -1.4607505229806534 2316 90-91 -1.5960559088540869 2316 92-93 -1.848930615298677 2316 94-95 -2.302404536559404 2316 96-97 -2.173486908645067 2316 98-99 -1.9921975221405006 2316 100-101 -1.0992045336167973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 6.0 6 25.0 7 43.0 8 72.0 9 119.0 10 180.0 11 330.0 12 507.0 13 635.0 14 787.0 15 1002.0 16 1325.0 17 1734.0 18 1743.0 19 1190.0 20 1278.0 21 1354.0 22 1334.0 23 1266.0 24 1303.0 25 1397.0 26 1338.0 27 1318.0 28 1478.0 29 1668.0 30 1975.0 31 2326.0 32 3040.0 33 3782.0 34 5910.0 35 9243.0 36 10796.0 37 12593.0 38 4316.0 39 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.78589929548281 19.761448404475757 14.39986531288852 14.05278698715292 2 14.643239547454106 27.592657094572804 33.54489982842473 24.21920352954836 3 28.9678393126669 29.068462401795735 30.314640659468246 11.649057626069121 4 12.011558754853773 19.287382122631165 36.81515022511191 31.885908897403148 5 9.688197427661029 43.514325889804816 31.237018976482577 15.560457706051576 6 23.8889533908691 37.907813769882736 14.340080240463383 23.863152598784783 7 19.495078498909916 40.57303559219268 20.435517370383273 19.496368538514133 8 23.990866519602154 26.05234980713908 19.43831675632442 30.51846691693435 9 21.37466620225241 24.247584400841106 24.324986777094058 30.05276261981243 10-11 24.72231897519254 26.286491995304257 22.667930905478798 26.323258124024406 12-13 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36.41894856375977 17.038738482979326 38-39 19.193893878393723 28.498890265304016 35.198590895013936 17.108624961288324 40-41 17.067600781869906 30.414223323205153 34.51387948107578 18.00429641384916 42-43 16.047582798972996 31.813900679938584 30.877856193634123 21.260660327454296 44-45 18.123234121608544 28.628839777579955 31.23556656474565 22.012359536065848 46-47 20.969011249870988 30.64299721333471 30.521725668283622 17.866265868510684 48-49 19.61271262425255 28.698871809426745 31.964109477703882 19.72430608861682 50-51 15.460034057484906 30.678182568759997 35.56814077093761 18.293642602817485 52-53 17.67289755795503 32.43933588761175 30.53459241198705 19.35317414244617 54-55 14.731607260342894 28.524710708618755 32.453526323258124 24.29015570778023 56-57 19.868673121206452 28.5410202989041 28.95899582669496 22.63131075319448 58-59 21.991085653836976 27.97053492527301 29.496681308899507 20.541698111990506 60-61 18.037037753739575 29.69664131689791 32.92438286536241 19.341938064000104 62-63 17.48435786621944 29.61233309681997 31.679674901631945 21.223634135328645 64-65 16.270504228241165 33.523405298363535 29.912467989859966 20.29362248353534 66-67 17.64648966638285 28.863818148979536 28.52452460200738 24.965167582630237 68-69 15.657575601197236 29.843766126535247 30.356589947362988 24.142068324904532 70-71 20.483380957602577 28.811930363212994 27.52704262963369 23.17764604955074 72-73 18.53915915218597 25.261555529754766 32.47223189751926 23.727053420540013 74-75 16.362217320071725 31.374408194331565 29.201336481029966 23.06203800456674 76-77 18.543768924354232 25.52342584881176 29.996190918892395 25.936614307941614 78-79 17.94628629674789 26.3323591837788 32.25854316256685 23.462811356906464 80-81 19.50552463024001 26.465333187127904 30.422555197925604 23.60658698470648 82-83 17.384573706412787 25.67565824270805 32.77861630352052 24.161151747358645 84-85 17.444560548008823 26.923771559786886 30.78873021401757 24.842937678186722 86-87 17.841048486022338 27.487894634902187 29.884434114532894 24.786622764542578 88-89 17.61727147926542 26.459579868935684 28.347190923925712 27.575957727873185 90-91 18.451486738499792 23.565711769581434 30.25927268959801 27.723528802320764 92-93 19.44670201375182 26.953442470683854 29.719932401924737 23.879923113639588 94-95 17.987667221383695 26.100081272495068 29.872157075222212 26.040094430899032 96-97 14.879316795025607 26.15877807448689 31.52921294683747 27.432692183650037 98-99 18.22128192062335 26.890403978507806 27.325795153289945 27.5625189475789 100-101 18.109047751123956 24.532196371095186 29.0093076957809 28.34944818199996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1576.0 1 974.0 2 403.0 3 407.5 4 296.5 5 253.5 6 417.0 7 712.0 8 979.0 9 1009.0 10 693.0 11 413.0 12 373.5 13 336.0 14 313.0 15 311.5 16 298.5 17 288.5 18 296.5 19 328.5 20 364.0 21 379.0 22 415.0 23 463.5 24 466.0 25 508.5 26 600.5 27 654.0 28 696.5 29 766.0 30 866.0 31 956.5 32 954.5 33 969.0 34 946.5 35 865.0 36 875.0 37 811.5 38 654.0 39 596.5 40 581.5 41 540.0 42 543.0 43 553.5 44 561.5 45 723.5 46 1650.5 47 4892.0 48 11658.5 49 12049.5 50 6532.5 51 3636.5 52 1642.0 53 699.5 54 441.5 55 410.0 56 382.0 57 358.0 58 379.0 59 418.5 60 458.0 61 480.5 62 468.0 63 476.0 64 465.0 65 425.0 66 410.5 67 390.5 68 338.5 69 281.5 70 241.5 71 202.5 72 142.5 73 106.5 74 89.0 75 60.5 76 45.5 77 30.0 78 19.0 79 13.5 80 7.0 81 4.0 82 3.0 83 3.5 84 2.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3883019208689707 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0019350594063237744 16-17 0.0038701188126475487 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0019350594063237744 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.015480475250590195 36-37 0.029670910896964536 38-39 0.02709083168853284 40-41 0.01354541584426642 42-43 0.012900396042158493 44-45 0.0077402376252950975 46-47 0.006450198021079247 48-49 0.004515138614755473 50-51 0.0012900396042158494 52-53 0.0 54-55 0.0 56-57 6.450198021079247E-4 58-59 0.0019350594063237744 60-61 0.0019350594063237744 62-63 0.002580079208431699 64-65 0.0032250990105396234 66-67 0.0038701188126475487 68-69 0.006450198021079247 70-71 6.450198021079247E-4 72-73 0.0 74-75 0.0 76-77 0.09094779209721739 78-79 0.017415534656913968 80-81 6.450198021079247E-4 82-83 0.0 84-85 0.0 86-87 0.09288285150354116 88-89 0.39088200007740237 90-91 0.3883019208689707 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.450198021079247E-4 100-101 6.450198021079247E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 77517.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.548550640504665 #Duplication Level Percentage of deduplicated Percentage of total 1 87.09159478734924 30.08888372873047 2 6.6315671558194245 4.582220674174698 3 2.2179903663044698 2.2988505747126435 4 0.996975467682312 1.3777622973025272 5 0.6235764161159031 1.0771830695202342 6 0.4107389567230499 0.8514261387824607 7 0.34352712744109626 0.8307855051150069 8 0.22030544042418132 0.6088986931898809 9 0.16429558268921995 0.5108556832694764 >10 0.9297636384003584 6.292813189364914 >50 0.1120197154699227 2.6420011094340596 >100 0.1605615921735559 12.6810893094418 >500 0.06347783876628953 16.091954022988507 >1k 0.033605914640976814 20.065276003973324 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3502 4.5177186939639045 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2114 2.727143723312306 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2067 2.666511861914161 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1811 2.3362617232349034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1433 1.8486267528413123 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1414 1.824116000361211 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1149 1.4822555052440112 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1034 1.3339009507591881 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1030 1.328740792342325 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 994 1.2822993665905544 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 982 1.266818891339964 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 930 1.19973683192074 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 927 1.1958667131080924 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 886 1.1429750893352426 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 833 1.0746029903118026 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 781 1.0075209308925783 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 773 0.9972006140588516 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 756 0.9752699407871821 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 677 0.8733568120541302 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 593 0.7649934852999987 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 587 0.7572532476747036 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 582 0.7508030496536243 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 581 0.7495130100494085 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 578 0.745642891236761 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 507 0.6540500793374358 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 507 0.6540500793374358 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 497 0.6411496832952772 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 471 0.6076086535856651 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 403 0.5198859604989874 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 356 0.45925409910084236 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 353 0.4553839802881948 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 352 0.454093940683979 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 338 0.43603338622495713 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 336 0.43345330701652546 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 335 0.43216326741230954 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 331 0.4270031089954462 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 324 0.41797283176593525 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 317 0.40894255453642425 TruSeq Adapter, Index 1 (95% over 21bp) CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 300 0.38701188126475483 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.38056168324367556 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.3715314060141646 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 260 0.33541029709612086 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 241 0.3108995446160197 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 215 0.27735851490640767 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 214 0.27606847530219175 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 205 0.26445811886424914 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 201 0.25929796044738573 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 201 0.25929796044738573 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 185 0.23865732677993212 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 182 0.2347872079672846 RNA PCR Primer, Index 1 (95% over 23bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 181 0.23349716836306875 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 179 0.23091708915463702 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.22962704955042118 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 164 0.2115664950913993 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 156 0.20124617825767252 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.188345782215514 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 145 0.18705574261129815 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 141 0.18189558419443477 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 139 0.17931550498600307 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 138 0.17802546538178723 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 133 0.17157526736070797 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 131 0.1689951881522763 TruSeq Adapter, Index 1 (96% over 28bp) TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 125 0.16125495052698116 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 124 0.15996491092276532 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 119 0.15351471290168608 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 119 0.15351471290168608 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 106 0.13674419804688004 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 104 0.13416411883844834 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 102 0.13158403963001664 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 98 0.12642388121315323 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 91 0.1173936039836423 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 88 0.11352348517099475 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.1070732871499155 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 79 0.10191312873305211 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 78 0.10062308912883626 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0012900396042158494 0.0 10-11 0.0 0.0 0.0 0.0012900396042158494 0.0 12-13 0.0 0.0 0.0 0.0012900396042158494 0.0 14-15 0.0 0.0 0.0 0.002580079208431699 0.0 16-17 0.0 0.0 0.0 0.002580079208431699 0.0 18-19 0.0 0.0 0.0 0.002580079208431699 0.0 20-21 0.0 0.0 0.0 0.004515138614755473 0.0 22-23 0.0 0.0 0.0 0.010320316833726795 0.0 24-25 0.0 0.0 0.0 0.025800792084316987 0.0 26-27 0.0 0.0 0.0 0.06127688120025285 0.0 28-29 0.0 0.0 0.0 0.1354541584426642 0.0 30-31 0.0 0.0 0.0 0.3102545248139118 0.0 32-33 0.0 0.0 0.0 0.5624572674381103 0.0 34-35 0.0 0.0 0.0 0.967529703161887 0.0 36-37 0.0 0.0 0.0 1.491285782473522 0.0 38-39 0.0 0.0 0.0 2.099539455861295 0.0 40-41 0.0 0.0 0.0 2.681347317362643 0.0 42-43 0.0 0.0 0.0 3.2109085748932493 0.0 44-45 0.0 0.0 0.0 3.746920030444935 0.0 46-47 0.0 0.0 0.0 4.366784060270651 0.0 48-49 0.0 0.0 0.0 4.960202278209941 0.0 50-51 0.0 0.0 0.0 5.512984248616433 0.0 52-53 0.0 0.0 0.0 5.9696582685088435 0.0 54-55 0.0 0.0 0.0 6.488254189403614 0.0 56-57 0.0 0.0 0.0 7.106183159823007 0.0 58-59 0.0 0.0 0.0 7.637034456957829 0.0 60-61 0.0 0.0 0.0 8.328495684817524 0.0 62-63 0.0 0.0 0.0 8.939974457215836 0.0 64-65 0.0 0.0 0.0 9.474050853361199 0.0 66-67 0.0 0.0 0.0 10.101655120812207 0.0 68-69 0.0 0.0 0.0 10.850523111059509 0.0 70-71 0.0 0.0 0.0 11.591650863681515 0.0 72-73 0.0 0.0 0.0 12.299237586593907 0.0 74-75 0.0 0.0 0.0 13.21258562637873 0.0 76-77 0.0 0.0 0.0 13.898886695821563 0.0 78-79 0.0 0.0 0.0 15.007030715842976 0.0 80-81 0.0 0.0 0.0 16.02809706257982 0.0 82-83 0.0 0.0 0.0 16.795025607286142 0.0 84-85 0.0 0.0 0.0 17.876078795619023 0.0 86-87 0.0 0.0 0.0 18.905530399783274 0.0 88-89 0.0 0.0 0.0 19.86983500393462 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGCTC 55 4.961027E-5 43.1217 8 GTAAGCG 35 4.327285E-6 40.657604 94-95 TGGGGGC 60 8.287099E-5 39.528225 6 ATGGGGG 85 3.6218444E-7 39.063187 5 GGGCTCT 65 1.3276252E-4 36.487595 9 GGGGGCT 65 1.3276252E-4 36.487595 7 ATACACT 30 0.0039674714 31.622581 94-95 CATGGGG 170 1.05501385E-10 30.692503 4 GTTGATC 90 2.1100277E-10 28.987364 94-95 GCAGAGA 150 0.0 26.879192 94-95 CACTCCC 45 8.635427E-4 26.531881 90-91 GCTCTCG 50 0.0016575143 23.72459 10-11 GGCTCTC 50 0.0016575143 23.72459 10-11 GAGTACT 995 0.0 22.420416 12-13 AGTACTT 915 0.0 21.786982 12-13 TCGGAAG 55 0.0028787265 21.57477 14-15 CTCGGAA 55 0.0028787265 21.57477 14-15 TCTCGGA 55 0.0028787265 21.57477 12-13 AGTACTC 110 0.002876646 21.56085 5 ATGGGCT 135 3.787524E-4 21.08172 5 >>END_MODULE