##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 741216 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28790527997237 33.0 31.0 34.0 31.0 34.0 2 32.36517965073609 34.0 31.0 34.0 31.0 34.0 3 32.40916278115961 34.0 31.0 34.0 31.0 34.0 4 35.854158571860296 37.0 35.0 37.0 35.0 37.0 5 35.90944744851703 37.0 35.0 37.0 35.0 37.0 6 36.02926542330441 37.0 37.0 37.0 35.0 37.0 7 35.98505698743686 37.0 37.0 37.0 35.0 37.0 8 35.98425155420282 37.0 37.0 37.0 35.0 37.0 9 37.68194021715667 39.0 38.0 39.0 35.0 39.0 10-11 37.615085211328406 39.0 38.0 39.0 35.0 39.0 12-13 37.56884497906144 39.0 38.0 39.0 35.0 39.0 14-15 38.86617801018866 40.5 38.5 41.0 36.0 41.0 16-17 38.838487026723655 40.0 38.0 41.0 36.0 41.0 18-19 38.86437556663644 40.0 38.5 41.0 35.5 41.0 20-21 38.80804515822648 40.0 39.0 41.0 35.0 41.0 22-23 38.72451012930104 40.0 38.5 41.0 35.0 41.0 24-25 38.72476646483616 41.0 38.0 41.0 35.0 41.0 26-27 38.61525520226223 40.0 38.0 41.0 35.0 41.0 28-29 38.49899084747226 40.0 38.0 41.0 35.0 41.0 30-31 38.28246219725424 40.0 38.0 41.0 34.5 41.0 32-33 37.998540236584205 40.0 37.0 41.0 34.0 41.0 34-35 37.669601708543794 40.0 37.0 41.0 33.5 41.0 36-37 37.403435705651255 40.0 36.0 41.0 33.0 41.0 38-39 37.16893591071968 40.0 36.0 41.0 33.0 41.0 40-41 37.05440182079178 40.0 36.0 41.0 33.0 41.0 42-43 36.82524594720028 40.0 35.0 41.0 32.0 41.0 44-45 36.485659375944394 40.0 35.0 41.0 31.0 41.0 46-47 36.03210130380348 40.0 35.0 41.0 30.0 41.0 48-49 35.84775355631827 40.0 35.0 41.0 28.0 41.0 50-51 34.84583913461123 38.5 34.0 40.0 25.5 40.5 52-53 35.042964533955015 38.5 35.0 40.0 26.5 40.5 54-55 35.57191898178129 40.0 35.0 41.0 27.0 41.0 56-57 35.484849220739974 40.0 35.0 41.0 26.5 41.0 58-59 35.349985024608216 40.0 35.0 41.0 26.0 41.0 60-61 35.091113116824246 39.0 35.0 41.0 25.5 41.0 62-63 34.78872690281915 39.0 35.0 41.0 23.0 41.0 64-65 34.463742013124374 39.0 35.0 40.5 22.0 41.0 66-67 34.07158372188404 38.0 35.0 40.0 19.5 41.0 68-69 33.65660954431637 37.0 34.5 40.0 17.0 41.0 70-71 33.23357563787074 37.0 34.0 39.5 10.5 41.0 72-73 32.7641375793291 36.0 34.0 39.0 7.0 41.0 74-75 32.282458824418256 36.0 33.5 39.0 2.0 40.0 76-77 31.824580823943357 35.0 33.0 37.5 2.0 40.0 78-79 31.35065284613392 35.0 32.5 37.0 2.0 39.0 80-81 30.849118205759183 35.0 32.0 36.5 2.0 39.0 82-83 30.38082151491603 35.0 31.5 36.0 2.0 38.0 84-85 29.94153795924535 35.0 31.0 36.0 2.0 37.0 86-87 29.57656270237016 35.0 31.0 35.0 2.0 37.0 88-89 29.141261791434616 35.0 31.0 35.0 2.0 36.0 90-91 28.739854509346806 34.0 30.0 35.0 2.0 36.0 92-93 28.32425635712127 34.0 29.0 35.0 2.0 36.0 94-95 27.888817159910204 34.0 29.0 35.0 2.0 35.0 96-97 27.37904065751414 34.0 28.0 35.0 2.0 35.0 98-99 26.810617282951256 34.0 26.5 35.0 2.0 35.0 100-101 24.540732391097873 31.5 13.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2086771979641 1101 2 0.2889342411257232 1101 3 0.31285362435806263 1101 4 0.207504900311406 1101 5 0.2057817251245524 1101 6 0.3024357809925746 1101 7 0.2192071509645288 1101 8 0.36354474795125213 1101 9 0.28247559861387117 1101 10-11 0.3199245134871589 1101 12-13 0.3418011325001302 1101 14-15 0.5354730137575814 1101 16-17 0.6060250834617307 1101 18-19 0.34688309929135386 1101 20-21 0.5173910022709194 1101 22-23 0.6124638979912262 1101 24-25 0.39647838171774197 1101 26-27 0.4621747752669947 1101 28-29 0.5318040755226932 1101 30-31 0.428359775276995 1101 32-33 0.28509107109090337 1101 34-35 0.18063784013555306 1101 36-37 0.2927996842935414 1101 38-39 0.5244479970191023 1101 40-41 0.11462094956229407 1101 42-43 0.28796255731982967 1101 44-45 0.2725045732448095 1101 46-47 0.37131832980453083 1101 48-49 0.50274535652391 1101 50-51 0.5385046697757687 1101 52-53 0.40262658755054304 1101 54-55 0.3904816619782281 1101 56-57 0.3658045075557723 1101 58-59 0.44919851877120465 1101 60-61 0.41551887065826776 1101 62-63 0.45764878770054906 1101 64-65 0.37235662119859114 1101 66-67 0.2540767184207411 1101 68-69 0.23701830984980887 1101 70-71 0.2351105909286133 1101 72-73 0.13406451995511048 1101 74-75 0.13723571406100632 1101 76-77 0.08392519721942904 1101 78-79 0.12251332815067784 1101 80-81 -0.05504913325871996 1101 82-83 0.03195275347206561 1101 84-85 -0.051675313081215535 1101 86-87 0.13127271975925936 1101 88-89 -0.09579019039267322 1101 90-91 -0.11566564303332072 1101 92-93 0.049148611123637664 1101 94-95 -0.017265424084982328 1101 96-97 0.07084848099412255 1101 98-99 0.22906045169212064 1101 100-101 0.05703649077852546 1102 1 0.09218813116586233 1102 2 0.21338466304146664 1102 3 0.17495972801059878 1102 4 0.21902302770051563 1102 5 0.18890774808122757 1102 6 0.2643957195554876 1102 7 0.36255770047629454 1102 8 0.35111408352348406 1102 9 0.37159385970026904 1102 10-11 0.3042469149450966 1102 12-13 0.33532715970115845 1102 14-15 0.36752773414720963 1102 16-17 0.3962476735283431 1102 18-19 0.5543499866646542 1102 20-21 0.4806746500846799 1102 22-23 0.29219920919553743 1102 24-25 0.3518887448491199 1102 26-27 0.3108037148936518 1102 28-29 0.4248475722372902 1102 30-31 0.42514865293601645 1102 32-33 0.0951812371779468 1102 34-35 0.02927341157208474 1102 36-37 0.20044509998864868 1102 38-39 0.21786605835538353 1102 40-41 0.20451513240394803 1102 42-43 0.04270629092268052 1102 44-45 0.01564396593018813 1102 46-47 0.0024809391299172034 1102 48-49 -0.10678826807851038 1102 50-51 0.20801638287836255 1102 52-53 0.010575209593085333 1102 54-55 -0.12033614628818867 1102 56-57 -0.027131553838863454 1102 58-59 0.05131114812465398 1102 60-61 -0.1366681676019681 1102 62-63 -0.1378585590184258 1102 64-65 -0.054530327664849665 1102 66-67 -0.09468353104141869 1102 68-69 -0.21381212538260996 1102 70-71 -0.3259869470782846 1102 72-73 -0.3662511541948916 1102 74-75 -0.3311676909754553 1102 76-77 -0.22979920067855275 1102 78-79 -0.15906138184027796 1102 80-81 -0.44472499691931944 1102 82-83 -0.5392994118138574 1102 84-85 -0.46063382713136747 1102 86-87 -0.5699562872465833 1102 88-89 -0.6795061791557373 1102 90-91 -0.5633764749010233 1102 92-93 -0.3664841563836774 1102 94-95 -0.47246489566237315 1102 96-97 -0.49145339368588736 1102 98-99 -0.6479499814711751 1102 100-101 -0.42303757327414715 1103 1 0.4287731939221686 1103 2 0.4062615115564441 1103 3 0.5356829789115594 1103 4 0.3897077549280539 1103 5 0.502821007681078 1103 6 0.4400636703445997 1103 7 0.4033753965120894 1103 8 0.3587020044878173 1103 9 0.5742805752464335 1103 10-11 0.5387119329642331 1103 12-13 0.44567760085936214 1103 14-15 0.5550448212604238 1103 16-17 0.6331111008925703 1103 18-19 0.6598526584678055 1103 20-21 0.7238795711732848 1103 22-23 0.7434642769226372 1103 24-25 0.6840721672425616 1103 26-27 0.6569215227723575 1103 28-29 0.571965752925756 1103 30-31 0.711898994678279 1103 32-33 0.6942877364944664 1103 34-35 0.506564327441346 1103 36-37 0.5222047619744856 1103 38-39 0.5408721475811831 1103 40-41 0.5206093289989511 1103 42-43 0.31275351349009384 1103 44-45 0.3843714420082307 1103 46-47 0.5187453855074509 1103 48-49 0.4579986090185386 1103 50-51 0.6305104800574313 1103 52-53 0.5823385996763477 1103 54-55 0.6593208687484662 1103 56-57 0.6446007687658337 1103 58-59 0.5861241219037012 1103 60-61 0.6297565869649517 1103 62-63 0.6080517181034821 1103 64-65 0.3288237173208586 1103 66-67 0.3312018920976314 1103 68-69 0.3636912920400235 1103 70-71 0.43855760368597174 1103 72-73 0.37837044391620367 1103 74-75 0.183834173073258 1103 76-77 0.3074618904112967 1103 78-79 0.3261157027875363 1103 80-81 0.35247644461168903 1103 82-83 0.33298218381183986 1103 84-85 0.5399465133681964 1103 86-87 0.3728894937898275 1103 88-89 0.18911318209690364 1103 90-91 0.05026463358661104 1103 92-93 0.14224953319007838 1103 94-95 -0.14334348395614782 1103 96-97 -0.2102529463141991 1103 98-99 -0.19173151496191565 1103 100-101 -0.25031730485113 1104 1 0.159304272750461 1104 2 0.17588284997034975 1104 3 0.06174156428902933 1104 4 -0.02038988736772751 1104 5 0.10772217462558586 1104 6 0.22639374893473985 1104 7 0.13192505119177156 1104 8 0.1721051957238302 1104 9 0.1286173220909177 1104 10-11 0.25582791295946805 1104 12-13 0.20143373327183411 1104 14-15 0.34894813157240634 1104 16-17 0.3282337491421572 1104 18-19 0.2307242945716368 1104 20-21 0.10981908058028722 1104 22-23 0.09334281943276324 1104 24-25 0.10247893075768388 1104 26-27 0.14648570472044753 1104 28-29 0.07961757478809517 1104 30-31 0.007764676983590846 1104 32-33 0.07564758407722394 1104 34-35 -0.01002771828382265 1104 36-37 -0.05165806250753491 1104 38-39 -0.0040575975962440225 1104 40-41 -0.09105297154879821 1104 42-43 -0.12823005402597687 1104 44-45 -0.07592862943734957 1104 46-47 0.020005209275012703 1104 48-49 -0.09952222927629606 1104 50-51 -0.06995867926621457 1104 52-53 -0.07618962132865192 1104 54-55 -0.2943109483508408 1104 56-57 -0.15941447295838884 1104 58-59 -0.12074179855354572 1104 60-61 -0.15046251282071665 1104 62-63 -0.19280086584507217 1104 64-65 -0.2777969066372279 1104 66-67 -0.21111770780710515 1104 68-69 -0.18486550719789108 1104 70-71 -0.14001586618780948 1104 72-73 -0.1461568244810323 1104 74-75 -0.35956663664334343 1104 76-77 -0.4199803577430288 1104 78-79 -0.264081963323445 1104 80-81 -0.08759500097059458 1104 82-83 -0.02174513221578067 1104 84-85 0.09153260529484797 1104 86-87 -0.1800659717972195 1104 88-89 -0.07904828491905391 1104 90-91 -0.15079276103115546 1104 92-93 -0.25344229819835107 1104 94-95 -0.30200745156748 1104 96-97 -0.395699481203458 1104 98-99 -0.44101229610027914 1104 100-101 -0.47673073662059906 1105 1 0.04503592662325673 1105 2 0.15427228312886143 1105 3 0.1936597966143978 1105 4 0.19353634642788364 1105 5 0.189136427421154 1105 6 0.2963100115224506 1105 7 0.2939760287565534 1105 8 0.19195572185112297 1105 9 0.19235105879636905 1105 10-11 0.09149029615355886 1105 12-13 0.32941838062753703 1105 14-15 0.3476510734557152 1105 16-17 0.21364967951334535 1105 18-19 0.21313213393906238 1105 20-21 0.28391820833521564 1105 22-23 0.23123034646953045 1105 24-25 0.2942185201286378 1105 26-27 0.22130157194693112 1105 28-29 0.14841113402867023 1105 30-31 0.12327471180741867 1105 32-33 -0.15579187947556505 1105 34-35 -0.31667416922180536 1105 36-37 -0.3006614129464893 1105 38-39 -0.1382860306180973 1105 40-41 -0.2505333943355552 1105 42-43 -0.5633394498631361 1105 44-45 -0.47998284905839483 1105 46-47 -0.48056851216261265 1105 48-49 -0.2891059454832785 1105 50-51 -0.6495422069815504 1105 52-53 -0.537195412734377 1105 54-55 -0.4391102283127637 1105 56-57 -0.4300731570039886 1105 58-59 -0.4675487501253386 1105 60-61 -0.45580248105986954 1105 62-63 -0.3917750000793987 1105 64-65 -0.21145845104350514 1105 66-67 -0.46013401704649226 1105 68-69 -0.4631451439744936 1105 70-71 -0.5900538001309101 1105 72-73 -0.654577688620968 1105 74-75 -0.7795377974220017 1105 76-77 -0.48097271911920814 1105 78-79 -0.4893813116135881 1105 80-81 -0.644097727030978 1105 82-83 -0.7461361864024205 1105 84-85 -0.767044471294021 1105 86-87 -0.7566901807213533 1105 88-89 -0.6057398796054727 1105 90-91 -0.5158763335132583 1105 92-93 -0.4343035636369912 1105 94-95 -0.410855484892803 1105 96-97 -0.33008200614325744 1105 98-99 -0.39236024696051075 1105 100-101 -0.23001991578661674 1106 1 0.19728037415148947 1106 2 0.22023011892591882 1106 3 0.21397976172353594 1106 4 0.11758262969149769 1106 5 0.2508628645217712 1106 6 0.19667059453473712 1106 7 0.1373239660322909 1106 8 0.25892019440210134 1106 9 0.33941694394285804 1106 10-11 0.3256027266857089 1106 12-13 0.37586684721357955 1106 14-15 0.46375571962399675 1106 16-17 0.5390378514213552 1106 18-19 0.5804003596812137 1106 20-21 0.5404519857341228 1106 22-23 0.579613670221093 1106 24-25 0.6177112633129127 1106 26-27 0.6968647488660977 1106 28-29 0.5849302414039883 1106 30-31 0.45093717791328203 1106 32-33 0.5057215559311814 1106 34-35 0.5796312315936376 1106 36-37 0.4378454165442278 1106 38-39 0.7087481355584728 1106 40-41 0.594049159790444 1106 42-43 0.6225909842682356 1106 44-45 0.6262338487765717 1106 46-47 0.8064198817781829 1106 48-49 0.8628455421143926 1106 50-51 0.9388050364417211 1106 52-53 0.914385799943517 1106 54-55 0.938949055323107 1106 56-57 0.9981274302374956 1106 58-59 0.840664921191248 1106 60-61 0.9980210865753278 1106 62-63 0.9830088593703152 1106 64-65 1.0791688136138546 1106 66-67 0.9302890567419624 1106 68-69 1.0390080013798908 1106 70-71 0.9905101807760275 1106 72-73 0.9323245795056536 1106 74-75 0.8167986615514948 1106 76-77 0.6376121742808252 1106 78-79 0.746512006917559 1106 80-81 0.8143911724309021 1106 82-83 0.9000254878305434 1106 84-85 1.1648346353764332 1106 86-87 0.9809135392089523 1106 88-89 1.0747950255790393 1106 90-91 1.2420903042979532 1106 92-93 1.3444180737667217 1106 94-95 1.0783909032196313 1106 96-97 0.8784724238294039 1106 98-99 0.9374113103748307 1106 100-101 0.9144027218660007 1107 1 0.16052164349239462 1107 2 0.2503220055179298 1107 3 0.2038126710016428 1107 4 0.19402719786135947 1107 5 0.25788792096581403 1107 6 0.2476145353212118 1107 7 0.10858919261697508 1107 8 0.24585465381868232 1107 9 0.27626512534979497 1107 10-11 0.24481355411536043 1107 12-13 0.37833237183095747 1107 14-15 0.41356402482913523 1107 16-17 0.32023763772208014 1107 18-19 0.2899320839927739 1107 20-21 0.18849604380955753 1107 22-23 0.25576000100053875 1107 24-25 0.23001493734375345 1107 26-27 0.11458336587975282 1107 28-29 0.2652983609118209 1107 30-31 0.3606379784667695 1107 32-33 0.3707614607899714 1107 34-35 0.27794067386719945 1107 36-37 0.23800282529730055 1107 38-39 0.2510571111324822 1107 40-41 0.2954075011238544 1107 42-43 0.3928090025554951 1107 44-45 0.3462417499181427 1107 46-47 0.42472514831889896 1107 48-49 0.6226139936339266 1107 50-51 0.5754098381251183 1107 52-53 0.4707141844875764 1107 54-55 0.41803047597292675 1107 56-57 0.350912377948724 1107 58-59 0.4360947453396733 1107 60-61 0.22318929287290246 1107 62-63 0.31384869340540433 1107 64-65 0.24324543421781897 1107 66-67 0.09544952565219944 1107 68-69 0.15663982865192594 1107 70-71 0.25670905619386275 1107 72-73 0.2626148134080992 1107 74-75 0.4108867244822463 1107 76-77 0.14175413282234928 1107 78-79 0.2498280300642186 1107 80-81 0.20301066286867808 1107 82-83 0.3541169896001115 1107 84-85 0.13683368560190345 1107 86-87 -0.038027707820443624 1107 88-89 -0.06133181844710478 1107 90-91 0.14090656591171324 1107 92-93 -0.09584643025242201 1107 94-95 -0.1416374672006242 1107 96-97 -0.004449442886933497 1107 98-99 -0.022878288979978123 1107 100-101 0.19363438702135127 1108 1 0.12403136041321261 1108 2 0.2206345357924846 1108 3 0.27448171442262037 1108 4 0.2793810952863254 1108 5 0.22840583381384505 1108 6 0.2628244711875922 1108 7 0.2816472616332675 1108 8 0.2968263458998308 1108 9 0.22081376808099407 1108 10-11 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-0.6650665055860507 2316 88-89 -0.710467095637533 2316 90-91 -0.9764306386867538 2316 92-93 -1.2585399815548115 2316 94-95 -1.3439098749687837 2316 96-97 -1.5190047016582469 2316 98-99 -1.8499746106086654 2316 100-101 -1.3562012180981249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2595.0 3 446.0 4 381.0 5 396.0 6 433.0 7 491.0 8 636.0 9 829.0 10 1085.0 11 1592.0 12 2691.0 13 4704.0 14 4320.0 15 4935.0 16 6777.0 17 9992.0 18 9024.0 19 4599.0 20 4215.0 21 4221.0 22 4323.0 23 4725.0 24 5232.0 25 5840.0 26 6528.0 27 7421.0 28 8548.0 29 10201.0 30 12078.0 31 14423.0 32 18146.0 33 23333.0 34 32066.0 35 54265.0 36 75880.0 37 139958.0 38 203583.0 39 50290.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.971545403233115 22.837852653862864 15.297425117523089 19.893176825380927 2 13.978313077257637 24.062407983208033 40.986744179135925 20.972534760398403 3 18.436086935381923 29.79368243626521 32.07187349745817 19.698357130894692 4 10.67292496792058 19.486459107179037 41.91747146619842 27.923144458701966 5 10.03779896032595 41.175604405105425 36.37967556117542 12.406921073393207 6 24.855016007313 41.625089960964026 19.002726171652945 14.517167860070025 7 23.283144732222432 36.786264077565015 24.416371836767762 15.514219353444783 8 23.036503565819448 35.472239815368745 22.24420427736282 19.24705234144899 9 24.924451717963883 18.788364889425402 24.84735061154275 31.439832781067967 10-11 21.97172391490304 29.12493476489369 29.396069757848636 19.50727156235463 12-13 24.346190434716267 28.353814358930734 29.244082377810948 18.055912828542052 14-15 21.31497411736746 29.377311190466067 29.80921728245524 19.498497409711238 16-17 17.20300822942475 32.61316302902239 30.934130847178437 19.24969789437442 18-19 17.0726733920709 31.41930966590617 34.05021164024736 17.457805301775565 20-21 18.252830502099705 30.02928582956147 33.59355428142352 18.124329386915306 22-23 17.943272133977224 29.970508965153037 33.65435588730062 18.431863013569117 24-25 18.399521269338678 30.747030559604266 33.50756661907277 17.345881551984288 26-27 17.836970037126864 29.861697866876852 35.13456378128041 17.16676831471587 28-29 16.76224180024786 31.070349196486763 34.74695833347403 17.420450669791347 30-31 17.57806749439843 30.42603019649738 33.98726663488277 18.008635674221416 32-33 17.480567730852524 30.933755055158542 34.11044403013546 17.475233183853476 34-35 18.408060869335714 30.3766029599562 33.73076431133883 17.484571859369257 36-37 18.11548474950333 29.759734947255833 34.28916533193613 17.835614971304707 38-39 17.24167139315063 31.290069233672625 33.95262131817064 17.515638055006107 40-41 17.334811148380172 30.8922511590031 34.28298306361628 17.489954629000447 42-43 17.0663836524021 31.010935627809133 33.188302077064456 18.734378642724312 44-45 17.654326844873395 30.300046087946647 33.066068427045494 18.97955864013447 46-47 18.588910232339348 30.99884636918084 32.57285429412299 17.83938910435682 48-49 17.974812770134328 30.95515702362811 32.593316666576285 18.476713539661276 50-51 16.938784363218833 31.126613354734022 33.45664937350119 18.47795290854595 52-53 17.586919550654407 32.026413592887145 32.014212692867424 18.372454163591023 54-55 17.44671420351382 30.935142092068585 32.34172852759801 19.276415176819587 56-57 18.31667234718997 30.95928115066848 31.648194237306743 19.075852264834804 58-59 18.747472284039063 30.402368347068524 32.161353805715606 18.68880556317681 60-61 18.02975626569768 31.07939089786833 32.75274914659474 18.138103689839248 62-63 17.491402297915883 30.90110694355503 32.89638371332636 18.711107045202734 64-65 17.523086214972437 31.353259252279813 32.333580300305506 18.79007423244224 66-67 17.850920544015143 30.75128160281363 31.268803733426616 20.128994119744615 68-69 17.51423614336283 30.494127569675573 32.184836973361 19.806799313600592 70-71 18.455663318088693 30.473799603268763 31.96140615868978 19.10913091995276 72-73 18.178003472186916 29.512978671336022 32.852704570582944 19.456313285894122 74-75 17.35563579367264 31.309701204159307 31.81890290118192 19.51576010098613 76-77 17.511149669915547 30.019859290487876 32.31303121907577 20.155959820520817 78-79 17.544393454354232 29.97185003672913 32.33037512502812 20.153381383888515 80-81 18.019339156268487 29.99188836987407 31.812837518695037 20.175934955162404 82-83 17.907746861660957 29.59425590086359 32.51157942393637 19.98641781353908 84-85 17.6344486841481 29.604736117982743 32.08414945746102 20.67666574040814 86-87 17.360840002602643 29.888154184830935 32.199693105168414 20.551312707398008 88-89 17.840777525616105 29.274275984176178 31.906518514903297 20.978427975304427 90-91 17.917841503918233 28.632425033575657 32.407542370410845 21.042191092095266 92-93 18.4361181368239 29.037370766240695 32.30411819376776 20.222392903167638 94-95 17.96378891078611 28.264228278871624 32.484019434970335 21.28796337537193 96-97 17.621528837040923 28.031729761464106 32.935253768943014 21.411487632551953 98-99 18.089692269625903 28.230524927888933 31.998061220685287 21.681721581799877 100-101 17.661476228207583 27.35509960070248 32.95678761723561 22.026636553854328 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5037.0 1 3021.0 2 1276.0 3 1574.5 4 1256.0 5 1162.0 6 2122.0 7 3920.5 8 5550.0 9 5534.0 10 4078.0 11 3023.5 12 2846.5 13 2721.5 14 2563.5 15 2423.0 16 2320.5 17 2261.5 18 2206.5 19 2283.0 20 2508.5 21 2891.5 22 3327.5 23 3779.5 24 4266.0 25 5099.5 26 6845.0 27 8800.0 28 9552.5 29 10598.0 30 12862.0 31 14887.5 32 17860.0 33 22394.0 34 27538.5 35 31072.0 36 35336.5 37 39883.0 38 39183.5 39 36731.5 40 34101.5 41 30969.0 42 28191.5 43 26219.0 44 25004.0 45 23824.5 46 22578.5 47 24638.0 48 33981.5 49 31646.5 50 18232.5 51 11081.0 52 7833.0 53 6297.0 54 5279.0 55 4473.5 56 3546.5 57 2820.0 58 2316.0 59 1881.0 60 1454.0 61 1108.5 62 797.0 63 546.5 64 386.0 65 268.5 66 234.0 67 229.5 68 199.0 69 175.0 70 155.0 71 129.0 72 110.5 73 98.0 74 83.0 75 64.5 76 49.5 77 36.0 78 26.5 79 21.0 80 16.5 81 9.5 82 6.0 83 4.0 84 5.0 85 3.5 86 2.0 87 1.5 88 0.5 89 0.5 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32716509087769285 2 0.11629538488106032 3 0.10847040538790312 4 0.11683503863920909 5 0.13275482450459786 6 0.08324159219444804 7 0.09120148512714243 8 0.09619328239001856 9 0.08499546690843154 10-11 0.08438835643051418 12-13 0.08803101929801839 14-15 0.08924524025385314 16-17 0.0783847083711091 18-19 0.09315773000043172 20-21 0.10192710357034926 22-23 0.110831390579804 24-25 0.12479493157190347 26-27 0.12364816733583732 28-29 0.11784688943573803 30-31 0.10779583819021715 32-33 0.10246675732849804 34-35 0.3221058368950481 36-37 0.47914508051634075 38-39 0.4522973060484393 40-41 0.4303064154038768 42-43 0.4646418857660925 44-45 0.4716573846220265 46-47 0.47826814315934896 48-49 0.4883866511246384 50-51 0.47826814315934896 52-53 0.4810338686698615 54-55 0.4684869187929025 56-57 0.447170595346026 58-59 0.42477496438285195 60-61 0.38457075940076846 62-63 0.4551304882787204 64-65 0.48575583905366315 66-67 0.4941879290247377 68-69 0.505183374347019 70-71 0.4976956784527048 72-73 0.49115237663515093 74-75 0.4976956784527048 76-77 0.4757047878081423 78-79 0.4562772525147865 80-81 0.4568843629927039 82-83 0.4701733367871174 84-85 0.4696336830289687 86-87 0.4727366921383241 88-89 0.48487890169667147 90-91 0.49951700988645686 92-93 0.49209677071191127 94-95 0.49641400077710146 96-97 0.49580689029918407 98-99 0.4908825497560765 100-101 0.4783355998791175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 741216.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.27636346751206 #Duplication Level Percentage of deduplicated Percentage of total 1 72.21918604674396 22.58753512125839 2 12.688337408582798 7.936901051785311 3 5.035047013537346 4.724338814142154 4 2.553185354026505 3.1941741252984572 5 1.5246622732958535 2.3842945712402157 6 0.977121312214944 1.8336480787612155 7 0.6959202282036683 1.5236097801184305 8 0.5031613722206257 1.2589646368307532 9 0.3664256695781044 1.0314416182996142 >10 2.7359436449687897 17.568037362750996 >50 0.40630124469271856 8.869868630209456 >100 0.2731654179043929 15.482011319081582 >500 0.010771507015157448 2.2089437499337206 >1k 0.008617205612125959 4.818925603808555 >5k 0.0021543014030314897 4.57730553648113 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9267 1.250242844191167 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7114 0.9597742088675907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6890 0.9295535984112594 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5595 0.7548406942105945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5101 0.6881934550792211 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4179 0.5638032638259293 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3141 0.423763113586323 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2499 0.33714868540344517 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2327 0.31394357380304794 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2097 0.2829134827094936 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1847 0.24918512282519537 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1767 0.2383920476622199 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1737 0.23434464447610412 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1727 0.2329955100807322 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1710 0.23070198160859992 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1570 0.2118141000733929 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1538 0.20749687000820274 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1536 0.20722704312912837 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1305 0.17606203859603678 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1276 0.17214954884945818 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1225 0.16526896343306136 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1143 0.15420606139101153 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1102 0.1486746103699866 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1028 0.13869101584423432 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1006 0.13572292017441612 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 981 0.13235008418598626 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 919 0.12398545093468032 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 899 0.12128718214393645 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 840 0.11332728921124206 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 754 0.10172473341104349 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3491343953719292E-4 0.0 5 0.0 0.0 0.0 1.3491343953719292E-4 0.0 6 0.0 0.0 0.0 1.3491343953719292E-4 0.0 7 0.0 0.0 0.0 1.3491343953719292E-4 0.0 8 0.0 0.0 0.0 1.3491343953719292E-4 0.0 9 0.0 0.0 0.0 5.396537581487717E-4 0.0 10-11 0.0 0.0 0.0 6.745671976859647E-4 0.0 12-13 0.0 0.0 0.0 8.094806372231576E-4 0.0 14-15 0.0 0.0 0.0 0.0010793075162975436 0.0 16-17 0.0 0.0 0.0 0.0012142209558347365 0.0 18-19 0.0 0.0 0.0 0.001281677675603333 0.0 20-21 0.0 0.0 0.0 0.0016864179942149117 0.0 22-23 0.0 0.0 0.0 0.002563355351206666 0.0 24-25 0.0 0.0 0.0 0.004519600224495964 0.0 26-27 0.0 0.0 0.0 0.009646310926909294 0.0 28-29 0.0 0.0 0.0 0.019292621853818588 0.0 30-31 0.0 0.0 0.0 0.0477593575961663 0.0 32-33 0.0 0.0 0.0 0.10030814229590296 0.0 34-35 0.0 0.0 0.0 0.17612949531580538 0.0 36-37 0.0 0.0 0.0 0.297551590899279 0.0 38-39 0.0 0.0 0.0 0.46329275137072057 0.0 40-41 0.0 0.0 0.0 0.6384103958899969 0.0 42-43 0.0 0.0 0.0 0.8175079868756205 0.0 44-45 0.0 0.0 0.0 1.0371470664421707 0.0 46-47 0.0 0.0 0.0 1.3172273669213832 0.0 48-49 0.0 0.0 0.0 1.5888081207097526 0.0 50-51 0.0 0.0 0.0 1.875769006605362 0.0 52-53 0.0 0.0 0.0 2.1480917843111857 0.0 54-55 0.0 0.0 0.0 2.4554920562966798 0.0 56-57 0.0 0.0 0.0 2.8441102188835643 0.0 58-59 0.0 0.0 0.0 3.242374692397358 0.0 60-61 0.0 0.0 0.0 3.678617299140871 0.0 62-63 0.0 0.0 0.0 4.103527176963261 0.0 64-65 0.0 0.0 0.0 4.557106160687303 0.0 66-67 0.0 0.0 0.0 5.0691701204507185 0.0 68-69 0.0 0.0 0.0 5.660630639381773 0.0 70-71 0.0 0.0 0.0 6.288180503389025 0.0 72-73 0.0 0.0 0.0 6.90655625350775 0.0 74-75 0.0 0.0 0.0 7.575861827051764 0.0 76-77 0.0 0.0 0.0 8.270666040668306 0.0 78-79 0.0 0.0 0.0 9.098967102706904 0.0 80-81 0.0 0.0 0.0 9.968888960842722 0.0 82-83 0.0 0.0 0.0 10.775199132236757 0.0 84-85 0.0 0.0 0.0 11.628256810430429 0.0 86-87 0.0 0.0 0.0 12.521248866727108 0.0 88-89 0.0 1.3491343953719292E-4 0.0 13.487566377412254 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCGA 15 6.6910853E-4 94.73422 7 CGTCGAG 20 0.0020969575 71.05066 8 GGCGCAT 50 0.0016378313 37.89369 6 CGCATGC 55 0.0026147116 34.448807 8 GCGCATG 55 0.0026147116 34.448807 7 CATTCCG 165 1.8189894E-12 34.44648 9 GGATATG 300 0.0 31.642172 1 GTTACAC 90 2.457304E-5 31.580204 3 GTACATG 4495 0.0 27.664902 1 CATATAG 175 5.1022653E-9 27.068747 3 CTCCTCG 125 7.552695E-6 26.52379 9 GATTGGC 365 0.0 25.952827 9 ACATGGG 4425 0.0 25.371216 3 CATGGGG 1245 0.0 24.731485 4 TACATGG 4760 0.0 24.680328 2 GGTACTC 175 1.5244223E-7 24.361872 3 GAGTACT 5350 0.0 24.168058 12-13 GATATGA 395 0.0 23.984964 2 CCGTATC 90 1.9301297E-7 23.777481 94-95 GGCATTC 240 2.382876E-10 23.683556 7 >>END_MODULE