##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939194_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 729197 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31729011501693 33.0 31.0 34.0 31.0 34.0 2 32.40425975422279 34.0 31.0 34.0 31.0 34.0 3 32.44916394335139 34.0 31.0 34.0 31.0 34.0 4 35.88465668399623 37.0 35.0 37.0 35.0 37.0 5 35.94254501869865 37.0 35.0 37.0 35.0 37.0 6 36.063427304281284 37.0 37.0 37.0 35.0 37.0 7 36.016431773581076 37.0 37.0 37.0 35.0 37.0 8 36.02355330589676 37.0 37.0 37.0 35.0 37.0 9 37.731512883349765 39.0 38.0 39.0 35.0 39.0 10-11 37.660650002674174 39.0 38.0 39.0 35.0 39.0 12-13 37.6096966937604 39.0 38.0 39.0 35.0 39.0 14-15 38.90083886796023 40.5 38.5 41.0 36.0 41.0 16-17 38.857833342704375 40.0 38.0 41.0 36.0 41.0 18-19 38.85905112061624 40.0 38.5 41.0 35.5 41.0 20-21 38.86890373931873 40.5 39.0 41.0 35.0 41.0 22-23 38.87302333937194 41.0 39.0 41.0 35.0 41.0 24-25 38.81782015011032 41.0 39.0 41.0 35.0 41.0 26-27 38.69605812969609 40.5 38.0 41.0 35.0 41.0 28-29 38.56191810992091 40.0 38.0 41.0 35.0 41.0 30-31 38.309314903928566 40.0 38.0 41.0 34.5 41.0 32-33 38.036158952930414 40.0 37.0 41.0 34.0 41.0 34-35 37.70654843615648 40.0 37.0 41.0 33.5 41.0 36-37 37.43283227989144 40.0 36.5 41.0 33.0 41.0 38-39 37.240338344782 40.0 36.0 41.0 33.0 41.0 40-41 37.09055851847992 40.0 36.0 41.0 33.0 41.0 42-43 36.85512008414736 40.0 36.0 41.0 32.5 41.0 44-45 36.508552558499275 40.0 35.0 41.0 31.0 41.0 46-47 36.175020604857124 40.0 35.5 41.0 30.0 41.0 48-49 35.955659444567104 40.0 35.5 41.0 28.5 41.0 50-51 34.83737247959056 38.5 34.0 40.0 26.0 40.5 52-53 35.01655793976113 38.5 35.0 40.0 26.5 40.5 54-55 35.57608643480431 40.0 35.0 41.0 27.0 41.0 56-57 35.50061094601321 40.0 35.0 41.0 26.5 41.0 58-59 35.352369112873475 40.0 35.0 41.0 26.0 41.0 60-61 35.09331634661141 39.0 35.0 41.0 25.0 41.0 62-63 34.78285154766133 39.0 35.0 41.0 22.5 41.0 64-65 34.45712475503876 39.0 35.0 40.5 22.0 41.0 66-67 34.064341323400946 38.0 35.0 40.0 19.5 41.0 68-69 33.639138668974226 37.0 34.5 40.0 15.5 41.0 70-71 33.1973952169304 37.0 34.0 39.0 10.5 41.0 72-73 32.73263192251203 36.0 34.0 39.0 4.5 41.0 74-75 32.259416179715494 36.0 33.0 39.0 2.0 40.0 76-77 31.799520568515778 35.0 33.0 37.5 2.0 39.5 78-79 31.310069843951634 35.0 32.5 37.0 2.0 39.0 80-81 30.79076847545999 35.0 32.0 36.5 2.0 39.0 82-83 30.365085841000443 35.0 31.5 36.0 2.0 38.0 84-85 29.9215513777484 35.0 31.0 36.0 2.0 37.0 86-87 29.52258854603077 35.0 31.0 35.0 2.0 37.0 88-89 29.063665237240418 34.5 30.5 35.0 2.0 36.0 90-91 28.611664611895 34.0 30.0 35.0 2.0 36.0 92-93 28.194587333738344 34.0 29.0 35.0 2.0 36.0 94-95 27.723115289832513 34.0 29.0 35.0 2.0 35.0 96-97 27.17268995895485 34.0 27.0 35.0 2.0 35.0 98-99 26.605830797438827 34.0 25.5 35.0 2.0 35.0 100-101 23.98718864723799 31.5 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3897247858108557 1101 2 0.4470512020593489 1101 3 0.3679624851698762 1101 4 0.3434415536559854 1101 5 0.3264082930492549 1101 6 0.2281615797336798 1101 7 0.2619540564616756 1101 8 0.3164839786130216 1101 9 0.38096586620076067 1101 10-11 0.39657783101358746 1101 12-13 0.2522203776062639 1101 14-15 0.5045038235024251 1101 16-17 0.5239290074918657 1101 18-19 0.5510349567052586 1101 20-21 0.6091003368247669 1101 22-23 0.5878916778646257 1101 24-25 0.5695758193071541 1101 26-27 0.5792734826875758 1101 28-29 0.6312575737427011 1101 30-31 0.6779356438397315 1101 32-33 0.4288343275227504 1101 34-35 0.5849101680774638 1101 36-37 0.771492432824239 1101 38-39 0.6131144971705211 1101 40-41 0.663919462631057 1101 42-43 0.6347982531349601 1101 44-45 0.6416295237822212 1101 46-47 0.9580071263468 1101 48-49 0.9841614733239226 1101 50-51 0.9260493954104092 1101 52-53 0.948469498041085 1101 54-55 0.9000335673362443 1101 56-57 0.9601710081837354 1101 58-59 1.055322942091955 1101 60-61 0.831149770532349 1101 62-63 0.9131050989558034 1101 64-65 0.9363223375913776 1101 66-67 1.241297835695157 1101 68-69 1.1342557811916834 1101 70-71 1.1292124155828631 1101 72-73 1.1853598868898416 1101 74-75 0.9718388375863398 1101 76-77 1.2350010095696113 1101 78-79 0.9737587815709965 1101 80-81 0.7255143656196523 1101 82-83 0.7326268338660533 1101 84-85 0.7595223232657169 1101 86-87 0.8439665911644525 1101 88-89 0.6130443017699037 1101 90-91 0.6426833175494124 1101 92-93 0.5544653139108426 1101 94-95 0.3137250096984303 1101 96-97 0.1493298720538867 1101 98-99 0.2349361146674056 1101 100-101 0.20568480166016911 1102 1 0.19926259534312152 1102 2 0.1704830162068589 1102 3 0.2553465492508238 1102 4 0.02511702183740283 1102 5 0.15523639724735006 1102 6 0.10718605386166757 1102 7 0.024659856585934392 1102 8 0.08895557708644475 1102 9 0.22661299942166835 1102 10-11 0.14046900657051964 1102 12-13 0.18864561280641112 1102 14-15 0.295558452063716 1102 16-17 0.19786339552771892 1102 18-19 0.3540714792933457 1102 20-21 0.3023153119734303 1102 22-23 0.22111536207515314 1102 24-25 0.3255806342534555 1102 26-27 0.26909081427540116 1102 28-29 0.32722533357229366 1102 30-31 0.18000751301519813 1102 32-33 0.1280347025116555 1102 34-35 0.22583332863484173 1102 36-37 0.358470721188489 1102 38-39 0.21426303937341373 1102 40-41 0.2836995726869773 1102 42-43 0.2234892312125325 1102 44-45 0.420551097848076 1102 46-47 0.39216911489742756 1102 48-49 0.1286375982906378 1102 50-51 -0.0039702415825786375 1102 52-53 -0.07274566571146579 1102 54-55 0.007597415299322563 1102 56-57 -0.05528757662145267 1102 58-59 -0.0996581420475664 1102 60-61 -0.0393958494197264 1102 62-63 -0.040931024235817404 1102 64-65 0.038576154046509714 1102 66-67 0.15064731869861703 1102 68-69 -0.1101055172906058 1102 70-71 -0.08594856338225298 1102 72-73 0.10716138684546905 1102 74-75 -0.07045003700317665 1102 76-77 0.03875866466857403 1102 78-79 -0.09056623839883216 1102 80-81 0.18100384817934057 1102 82-83 -0.030163560648901466 1102 84-85 -0.09026022754638774 1102 86-87 -0.10131054560664765 1102 88-89 -0.036766816983977435 1102 90-91 -0.23706644946931377 1102 92-93 -0.08634967676990968 1102 94-95 -0.16664909165951514 1102 96-97 -0.5109085454485545 1102 98-99 -0.49007109665770443 1102 100-101 -0.5972421642579988 1103 1 0.16885988401935492 1103 2 0.2830727291255215 1103 3 0.3388294041152591 1103 4 0.30990569807344315 1103 5 0.28138272898578975 1103 6 0.16704450681222482 1103 7 0.15240108465610547 1103 8 0.20149544949473608 1103 9 0.19725693881560602 1103 10-11 0.2550274196486697 1103 12-13 0.22939023003129222 1103 14-15 0.4625241617925795 1103 16-17 0.3633837304559506 1103 18-19 0.34559859253099035 1103 20-21 0.26052404403085205 1103 22-23 0.20431911008153492 1103 24-25 0.3951081462151791 1103 26-27 0.25246900406806105 1103 28-29 0.18213960789445593 1103 30-31 0.16032051142030213 1103 32-33 0.2649715047445156 1103 34-35 0.2764214466571744 1103 36-37 0.2281128663240537 1103 38-39 0.22001961832078365 1103 40-41 0.29476416598841126 1103 42-43 0.3817328516272056 1103 44-45 0.42635751730581006 1103 46-47 0.27581644344607525 1103 48-49 0.30995737500515474 1103 50-51 0.14360750961359514 1103 52-53 0.08360401530926964 1103 54-55 0.07670128292293299 1103 56-57 0.1367728300771205 1103 58-59 0.18889349272117073 1103 60-61 0.2746990469120121 1103 62-63 0.07888556268603253 1103 64-65 -0.05726714738889882 1103 66-67 0.028014742941046222 1103 68-69 0.09518754491034542 1103 70-71 -0.040145134355142886 1103 72-73 0.10008403437337421 1103 74-75 0.004460250078160755 1103 76-77 -0.07118950120064582 1103 78-79 0.12115624963944427 1103 80-81 0.16505488486195574 1103 82-83 0.20765043456640697 1103 84-85 0.13222781073112344 1103 86-87 0.08120540335666604 1103 88-89 0.15170507296817703 1103 90-91 0.309011102364817 1103 92-93 0.11869418287921363 1103 94-95 0.08674006304542559 1103 96-97 0.25449217225479615 1103 98-99 0.2876900676166194 1103 100-101 0.4184326762523689 1104 1 0.014913941827522592 1104 2 0.016499325448080526 1104 3 -0.021723823999565184 1104 4 0.010930846835350394 1104 5 -0.0010801084414424622 1104 6 -0.041165116638453014 1104 7 0.029839626578564094 1104 8 -0.06421725585126836 1104 9 -0.08428319031394693 1104 10-11 0.0730207275331125 1104 12-13 0.08061095822672826 1104 14-15 0.08024884250326636 1104 16-17 0.09807160949187477 1104 18-19 0.15054872411614184 1104 20-21 0.14862705994756453 1104 22-23 0.27062884738251114 1104 24-25 0.07009903075013568 1104 26-27 0.09979674658283955 1104 28-29 0.20641774987744554 1104 30-31 0.32676766280647485 1104 32-33 0.3501087079958154 1104 34-35 0.2193387923329695 1104 36-37 0.047453030675264074 1104 38-39 0.039492483999758576 1104 40-41 0.18816159282285128 1104 42-43 0.37857282374752543 1104 44-45 0.620552888385852 1104 46-47 0.636962486838172 1104 48-49 0.5356984561117102 1104 50-51 0.6308086525812939 1104 52-53 0.7265989974627942 1104 54-55 0.7432104442753413 1104 56-57 0.7056410985852324 1104 58-59 0.8073784156179826 1104 60-61 0.7912755510877645 1104 62-63 0.6831726837121224 1104 64-65 0.7096262277143524 1104 66-67 0.6375367453172629 1104 68-69 0.5659734096145286 1104 70-71 0.5329559344486299 1104 72-73 0.5687466778334382 1104 74-75 0.6972839607049366 1104 76-77 0.6356472215974414 1104 78-79 0.6984367901679214 1104 80-81 0.3903023666372256 1104 82-83 0.5691921015431269 1104 84-85 0.5461071294152227 1104 86-87 0.7359909604914101 1104 88-89 0.5374343473771575 1104 90-91 0.28115994612698714 1104 92-93 0.35661402135565723 1104 94-95 0.12778694009950797 1104 96-97 0.06003511911721304 1104 98-99 0.24554065336275954 1104 100-101 0.2879575062830533 1105 1 0.12280633372132144 1105 2 0.13082995209497028 1105 3 0.07423095785297562 1105 4 0.17033389466462978 1105 5 0.2837860981124223 1105 6 0.2502731061698569 1105 7 0.23809920091449754 1105 8 0.22487501277242927 1105 9 0.2179244433540788 1105 10-11 0.31563348025473204 1105 12-13 0.3085029678876623 1105 14-15 0.24216387691617314 1105 16-17 0.13274849133174627 1105 18-19 0.17374667534800636 1105 20-21 0.28329725271237294 1105 22-23 0.2937288514437384 1105 24-25 0.2822490189248086 1105 26-27 0.33771878541291755 1105 28-29 0.17125829905050693 1105 30-31 0.24102447073207145 1105 32-33 0.1752861476567773 1105 34-35 0.08624292116456189 1105 36-37 0.1371045537537796 1105 38-39 0.35847058444577584 1105 40-41 0.18751657486986062 1105 42-43 0.22344122814033796 1105 44-45 0.16184359319935737 1105 46-47 0.07431929882498878 1105 48-49 -0.06373345029857802 1105 50-51 -0.1387957125179824 1105 52-53 -0.08395564630811947 1105 54-55 -0.22345526680917516 1105 56-57 -0.0023616630471963163 1105 58-59 0.03609580936321066 1105 60-61 0.1233749889665674 1105 62-63 0.18615887793897912 1105 64-65 0.1077384751718995 1105 66-67 0.13574741773653187 1105 68-69 0.008126049529671775 1105 70-71 0.11616205590790685 1105 72-73 -0.01854097389509235 1105 74-75 -0.019382347618424944 1105 76-77 0.07895492143952865 1105 78-79 -0.09192043033027275 1105 80-81 -0.08647937601016409 1105 82-83 -0.21945104346885458 1105 84-85 -0.12247786842277364 1105 86-87 -0.3795616168331044 1105 88-89 -0.2752751341478543 1105 90-91 0.04923776873749475 1105 92-93 -0.0011619089632937118 1105 94-95 0.1341128939496663 1105 96-97 0.21946325097799857 1105 98-99 -0.014388442441518379 1105 100-101 -0.08631893885578634 1106 1 0.0935379292001528 1106 2 0.1403033225697854 1106 3 0.07540251940007892 1106 4 -0.015034195758829583 1106 5 0.21759401704311188 1106 6 0.0977545927461918 1106 7 0.16800726428428447 1106 8 0.1517108795216373 1106 9 0.26383314867540264 1106 10-11 0.27160362950846206 1106 12-13 0.20069637871371526 1106 14-15 0.3133608872561737 1106 16-17 0.2603879438388006 1106 18-19 0.2895879757583941 1106 20-21 0.26562295140784187 1106 22-23 0.27595137935715996 1106 24-25 0.21869237816547837 1106 26-27 0.35156086533588393 1106 28-29 0.3466234089847049 1106 30-31 0.3149004823789099 1106 32-33 0.40881092010788933 1106 34-35 0.19351302227192235 1106 36-37 0.3542263182034162 1106 38-39 0.5396189898840191 1106 40-41 0.7274643016736206 1106 42-43 0.7401869924065565 1106 44-45 0.6574496642267107 1106 46-47 0.7005670921166001 1106 48-49 0.47558378839276116 1106 50-51 0.583731054568247 1106 52-53 0.41267754717149074 1106 54-55 0.34179566745957146 1106 56-57 0.3743790573141368 1106 58-59 0.3261593167112622 1106 60-61 0.5440389502660565 1106 62-63 0.6164727463847655 1106 64-65 0.5250402153190947 1106 66-67 0.45946752686829484 1106 68-69 0.5648011991273023 1106 70-71 0.42269735177878687 1106 72-73 0.46869909305127777 1106 74-75 0.4977795388020141 1106 76-77 0.2876098037102075 1106 78-79 0.4413269273513656 1106 80-81 0.3554343273623708 1106 82-83 0.42488602693947186 1106 84-85 0.5097790497513337 1106 86-87 0.5215193836942156 1106 88-89 0.4668196912642557 1106 90-91 0.5387166416540161 1106 92-93 0.47860515433771056 1106 94-95 0.6089793451040322 1106 96-97 0.4627903940644096 1106 98-99 0.3835492882598146 1106 100-101 0.40863715624475105 1107 1 -0.07237165332202267 1107 2 0.048414938403659846 1107 3 0.03079944780154875 1107 4 0.014600240754255367 1107 5 0.05490744987893237 1107 6 0.1579535977213169 1107 7 0.06826683599096839 1107 8 0.10559363963411528 1107 9 0.14047169507384183 1107 10-11 0.07867695851560086 1107 12-13 0.1854681719215847 1107 14-15 0.20046882582420267 1107 16-17 0.22406953587859846 1107 18-19 0.31817330995696835 1107 20-21 0.2306024019810593 1107 22-23 0.25021051844434083 1107 24-25 0.2658695358552876 1107 26-27 0.13697077232269095 1107 28-29 0.0983971489803821 1107 30-31 0.0785820744187049 1107 32-33 -0.027345255535635715 1107 34-35 0.12308256585833988 1107 36-37 0.1375920980000842 1107 38-39 0.023147001997365635 1107 40-41 0.19944137283953012 1107 42-43 0.1543769123719514 1107 44-45 0.07551152881680423 1107 46-47 0.009662917338317811 1107 48-49 -0.06481205845992832 1107 50-51 -0.13741322403871692 1107 52-53 -0.18029238577248918 1107 54-55 -0.21830599812554397 1107 56-57 -0.3519242089631689 1107 58-59 -0.24904076981263046 1107 60-61 -0.24844428572788502 1107 62-63 -0.2426663407880696 1107 64-65 -0.042178041224275375 1107 66-67 -0.1497364532312062 1107 68-69 0.014260424738381516 1107 70-71 -0.1738633231076534 1107 72-73 -0.22486569172051674 1107 74-75 -0.2531397776591575 1107 76-77 -0.26718145737567056 1107 78-79 -0.27485304236526886 1107 80-81 -0.2308191876711554 1107 82-83 -0.15320179482128893 1107 84-85 -0.04804054791252099 1107 86-87 -0.07923770045028178 1107 88-89 0.04577275204729858 1107 90-91 -0.13389854329082596 1107 92-93 -0.1670037576242187 1107 94-95 -0.12772842041619015 1107 96-97 -0.15218905650881354 1107 98-99 -0.17158806843497132 1107 100-101 -0.4251287965997257 1108 1 -0.04242131950017125 1108 2 -0.02675604352111094 1108 3 0.008506661610375943 1108 4 -0.11315175403486677 1108 5 0.085806326283425 1108 6 -0.05009883673561433 1108 7 -0.08764425340815762 1108 8 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-1.2016354440712043 2316 92-93 -1.3019535904001351 2316 94-95 -1.4774385653437285 2316 96-97 -1.6010519327629957 2316 98-99 -1.4884331074539254 2316 100-101 -1.3821856527870118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2473.0 3 400.0 4 332.0 5 377.0 6 404.0 7 496.0 8 618.0 9 801.0 10 988.0 11 1565.0 12 2714.0 13 4966.0 14 4386.0 15 4764.0 16 6752.0 17 10434.0 18 8269.0 19 4515.0 20 4266.0 21 4028.0 22 4232.0 23 4610.0 24 5209.0 25 5737.0 26 6474.0 27 7358.0 28 8387.0 29 9885.0 30 11880.0 31 14442.0 32 17739.0 33 22794.0 34 31612.0 35 53158.0 36 74712.0 37 139421.0 38 201242.0 39 46745.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02389705376149 22.823503818076173 15.308516526638414 19.844082601523926 2 13.953312746380922 24.08096741284071 40.91402773334358 21.051692107434782 3 18.528699773851027 29.76172658630391 32.04724550001991 19.66232813982515 4 10.748813499736332 19.314960889435753 42.154129680084374 27.782095930743537 5 10.04817543107947 41.12695901517773 36.3611893893201 12.46367616442269 6 24.927433599169966 41.545585423863265 19.041011853541445 14.485969123425328 7 23.239844517769008 36.859225992763925 24.375765195478277 15.525164293988789 8 23.02074540725643 35.34885636052951 22.258319093597954 19.3720791386161 9 24.84161405435081 18.830908591819927 24.87592643425748 31.45155091957178 10-11 22.031455764087944 29.01124812228207 29.43753898687982 19.519757126750164 12-13 24.300920896506714 28.372588728676945 29.275779646396057 18.050710728420285 14-15 21.31157103056421 29.458298450516722 29.76531298464241 19.46481753427666 16-17 17.213958480301063 32.615719073643525 30.935689208885865 19.234633237169547 18-19 16.989419401949487 31.539337355475016 34.04190924307924 17.42933399949626 20-21 18.232952673767432 30.0293730097727 33.58131107938948 18.156363237070387 22-23 17.871296302651356 29.994440593277922 33.650951619434586 18.483311484636133 24-25 18.406892305326764 30.719659970085385 33.48796310851153 17.385484616076326 26-27 17.85072467150559 29.87099103650609 35.12264569281396 17.155638599174353 28-29 16.702039303438195 31.050814417209498 34.83330702121672 17.413839258135592 30-31 17.565779359117965 30.424600821418373 34.00794239089851 18.001677428565152 32-33 17.462318714255026 30.963546903514903 34.10495235833614 17.469182023893925 34-35 18.2733670388586 30.352105310959615 33.79795399938901 17.576573650792778 36-37 18.141273525881484 29.868174400559266 34.212331529558895 17.77822054400035 38-39 17.253700973347204 31.319639887039063 33.86195026492882 17.56470887468491 40-41 17.268191841234838 31.024875964718852 34.27081036383682 17.43612183020948 42-43 17.07918778786755 31.008565201267757 33.16206607047094 18.750180940393754 44-45 17.588612782631294 30.357732510966084 33.08444935885549 18.96920534754713 46-47 18.674322143551912 31.074581615412246 32.465554834800386 17.78554140623546 48-49 17.93449336109311 30.980876087526028 32.5822107628883 18.50241978849256 50-51 16.92055694314131 31.27059117127787 33.340962565517756 18.467889320063065 52-53 17.608262688641492 32.015436701046276 32.014402624073725 18.361897986238503 54-55 17.399258687876163 31.003928526867913 32.28685208431133 19.309960700944597 56-57 18.222714718092057 31.02965174293921 31.6594446190232 19.08818891994553 58-59 18.828568357399377 30.32177023931911 32.184327809029796 18.66533359425171 60-61 18.087410432369335 31.100498238775852 32.67799037954983 18.13410094930498 62-63 17.50485045437471 30.952412131822083 32.891491114105435 18.651246299697775 64-65 17.525332539127724 31.409384035639974 32.268946147068675 18.79633727816362 66-67 17.821436746896573 30.751648588047427 31.271115854852543 20.155798810203457 68-69 17.46173059497391 30.53401318792867 32.133197803758875 19.871058413338538 70-71 18.501338103292888 30.512271865628364 31.853133423482628 19.133256607596124 72-73 18.15007763397031 29.529383699348315 32.81689966050651 19.503639006174865 74-75 17.323094701597714 31.322380378453367 31.780002261563624 19.5745226583853 76-77 17.538485293833805 30.041277080329003 32.205704343606186 20.214533282231002 78-79 17.71587152492311 29.871620024492053 32.25804450882548 20.154463941759364 80-81 18.11095849721332 29.97951083761598 31.766265038652357 20.14326562651834 82-83 17.928485121687782 29.491598790462938 32.51592394005251 20.06399214779677 84-85 17.601795358864997 29.613289294841927 32.12167746951447 20.66323787677861 86-87 17.370063294323774 29.8771540092953 32.199117615231444 20.553665081149486 88-89 17.763277618162668 29.3502776567644 31.89888827003568 20.98755645503725 90-91 17.89478982164366 28.651464998541794 32.315529873946076 21.138215305868474 92-93 18.382891874467784 28.99990002103006 32.3115482605383 20.305659843963856 94-95 17.97506458621044 28.259767910219825 32.457337248610195 21.30783025495954 96-97 17.61915136839812 28.056194850735878 32.8247316913751 21.499922089490905 98-99 18.00917412848882 28.285218456090565 31.9894124517455 21.71619496367511 100-101 17.726858837339627 27.293919941216977 32.97811697187472 22.001104249568673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4852.0 1 2917.0 2 1277.5 3 1535.0 4 1185.5 5 1105.5 6 2007.0 7 3792.0 8 5474.5 9 5505.5 10 4006.0 11 2879.5 12 2744.5 13 2708.0 14 2559.0 15 2408.5 16 2281.5 17 2192.0 18 2206.0 19 2288.5 20 2455.5 21 2802.0 22 3251.5 23 3695.5 24 4178.0 25 5048.0 26 6753.5 27 8720.5 28 9547.5 29 10521.0 30 12608.5 31 14732.0 32 17940.0 33 22239.5 34 26958.0 35 30686.0 36 34826.5 37 39149.0 38 38779.0 39 36069.5 40 33306.5 41 30365.0 42 27578.5 43 25733.5 44 24835.5 45 23607.0 46 22295.5 47 24080.0 48 33096.0 49 31090.0 50 17982.5 51 11016.5 52 7770.0 53 6220.5 54 5219.5 55 4350.0 56 3472.5 57 2785.0 58 2241.0 59 1823.0 60 1376.5 61 1041.0 62 768.0 63 506.0 64 350.0 65 266.0 66 238.0 67 214.0 68 182.0 69 158.0 70 142.0 71 130.5 72 110.0 73 95.5 74 75.5 75 55.0 76 42.5 77 30.5 78 25.0 79 17.0 80 10.5 81 10.0 82 7.0 83 3.0 84 3.5 85 4.0 86 3.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39975479877179965 2 0.12397198562254096 3 0.12561763144938884 4 0.1378228379985107 5 0.14056558104325717 6 0.07597398233947754 7 0.08571072014832755 8 0.0825565656468691 9 0.08187087988568247 10-11 0.08043093978719057 12-13 0.08413364289759831 14-15 0.07775676531856275 16-17 0.07940241114541065 18-19 0.08865916892143001 20-21 0.08735636597517543 22-23 0.0962702808706015 24-25 0.10840691884360468 26-27 0.1094354474853846 28-29 0.10449851000484095 30-31 0.09613314371836418 32-33 0.09400751785868565 34-35 0.34435138926792075 36-37 0.5428574171314473 38-39 0.5229725300570354 40-41 0.4932823365976547 42-43 0.5238639215465779 44-45 0.5338063650837839 46-47 0.531749307800224 48-49 0.5385375968359717 50-51 0.5370976567374797 52-53 0.5365491081285304 54-55 0.529555113364427 56-57 0.4993163712960969 58-59 0.48752257620368705 60-61 0.43054208944907896 62-63 0.5141957523138465 64-65 0.5408689284240061 66-67 0.5527998606686534 68-69 0.5677478102625216 70-71 0.5627423042058594 72-73 0.548411471797059 74-75 0.5533484092776026 76-77 0.5286637218748843 78-79 0.5013048600035381 80-81 0.5062417974840818 82-83 0.5205040613167635 84-85 0.517144201086949 86-87 0.5178984554242544 88-89 0.5267438017435617 90-91 0.547382943155279 92-93 0.5546512122238572 94-95 0.5490285889821269 96-97 0.5493714318627202 98-99 0.5493028632866015 100-101 0.5235210786659846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 729197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.427882490439877 #Duplication Level Percentage of deduplicated Percentage of total 1 71.9787828156055 22.621407281337433 2 12.90596021584104 8.112140021794886 3 4.989981536026811 4.704736600311459 4 2.60848079068748 3.2791611107318333 5 1.525212224604919 2.39670952839329 6 0.9990160054116187 1.8838174574487003 7 0.6785480347128993 1.4927729529353144 8 0.5007538556404897 1.2590106665363203 9 0.39790522977663195 1.125478692337632 >10 2.729590980040871 17.684696998031132 >50 0.3998496330418632 8.855352778810358 >100 0.2645293423474656 15.119085711543958 >500 0.010912926664499405 2.2335589796441893 >1k 0.008730341331599525 5.3270127866583055 >5k 0.001746068266319905 3.9050584334851575 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9236 1.2665987380639252 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7144 0.9797078155834431 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6747 0.9252643661452256 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5338 0.7320381186428359 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4940 0.6774575320523809 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4233 0.5805015654205928 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3153 0.4323934410042828 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2425 0.3325575941755109 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2258 0.30965568975187774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1954 0.2679659954717312 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1778 0.24382985667796223 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1773 0.24314417091677556 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1721 0.23601303900043472 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1668 0.22874476993185655 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1602 0.21969371788419317 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1524 0.2089970200096819 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1522 0.20872274570520724 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1454 0.19939741935306918 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1281 0.17567269201601213 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1175 0.16113615387885577 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1147 0.15729631361621071 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1125 0.15427929626698958 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1049 0.14385687269695296 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1048 0.14371973554471562 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 995 0.13645146647613746 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 975 0.13370872343139098 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 872 0.1195835967509466 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 847 0.11615516794501349 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 777 0.1065555672884008 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3713715223732408E-4 0.0 5 0.0 0.0 0.0 1.3713715223732408E-4 0.0 6 0.0 0.0 0.0 1.3713715223732408E-4 0.0 7 0.0 0.0 0.0 1.3713715223732408E-4 0.0 8 0.0 0.0 0.0 4.114114567119722E-4 0.0 9 0.0 0.0 0.0 6.856857611866204E-4 0.0 10-11 0.0 0.0 0.0 6.856857611866204E-4 0.0 12-13 0.0 0.0 0.0 6.856857611866204E-4 0.0 14-15 0.0 0.0 0.0 9.599600656612685E-4 0.0 16-17 0.0 0.0 0.0 9.599600656612685E-4 0.0 18-19 0.0 0.0 0.0 0.0012342343701359166 0.0 20-21 0.0 0.0 0.0 0.0014399400984919028 0.0 22-23 0.0 0.0 0.0 0.001714214402966551 0.0 24-25 0.0 0.0 0.0 0.005279780361136977 0.0 26-27 0.0 0.0 0.0 0.008708209167070078 0.0 28-29 0.0 0.0 0.0 0.01954204419381868 0.0 30-31 0.0 0.0 0.0 0.04847798331589406 0.0 32-33 0.0 0.0 0.0 0.09688739805566945 0.0 34-35 0.0 0.0 0.0 0.18362664684577693 0.0 36-37 0.0 0.0 0.0 0.3058844180653514 0.0 38-39 0.0 0.0 0.0 0.48039144428734626 0.0 40-41 0.0 0.0 0.0 0.6616867595450886 0.0 42-43 0.0 0.0 0.0 0.849084678077392 0.0 44-45 0.0 0.0 0.0 1.075360979268977 0.0 46-47 0.0 0.0 0.0 1.3448354834153184 0.0 48-49 0.0 0.0 0.0 1.6160242019646267 0.0 50-51 0.0 0.0 0.0 1.9055892989137366 0.0 52-53 0.0 0.0 0.0 2.188091832522624 0.0 54-55 0.0 0.0 0.0 2.5014502253849096 0.0 56-57 0.0 0.0 0.0 2.900450769819404 0.0 58-59 0.0 0.0 0.0 3.3106279921612405 0.0 60-61 0.0 0.0 0.0 3.7509068194191695 0.0 62-63 0.0 0.0 0.0 4.1693122708952455 0.0 64-65 0.0 0.0 0.0 4.6148023099381925 0.0 66-67 0.0 0.0 0.0 5.118918481562596 0.0 68-69 0.0 0.0 0.0 5.695100226687712 0.0 70-71 0.0 0.0 0.0 6.327439635654014 0.0 72-73 0.0 0.0 0.0 6.930500262617647 0.0 74-75 0.0 0.0 0.0 7.582861695810597 0.0 76-77 0.0 0.0 0.0 8.267313222627081 0.0 78-79 0.0 0.0 0.0 9.102135636871791 0.0 80-81 0.0 0.0 0.0 9.978853451125005 0.0 82-83 0.0 0.0 0.0 10.805859047692188 0.0 84-85 0.0 0.0 0.0 11.652612394181546 0.0 86-87 0.0 0.0 0.0 12.559226107622495 0.0 88-89 0.0 0.0 0.0 13.500946932036198 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACCG 20 0.002097716 71.04406 5 TTACGCA 50 2.874724E-5 47.36271 3 TACGCAA 40 5.464292E-4 47.362705 4 GACGTGA 50 0.001644096 37.86417 7 CGCAAGA 50 0.001644096 37.86417 6 GGACGTG 80 1.1038244E-5 35.497658 6 ACTTACG 55 0.0025799563 34.54285 1 ACGCAAG 55 0.0026158744 34.445606 5 TAGGACG 85 1.6640231E-5 33.432503 4 TCTATAC 85 1.6640231E-5 33.432503 3 CATATAG 230 0.0 32.94797 3 GTAAGCG 40 4.4555598E-4 29.731323 94-95 CCGTATC 45 8.8701607E-4 26.428753 92-93 CGCGGTA 45 8.8826695E-4 26.422377 42-43 TAACGGC 45 8.8844576E-4 26.421465 36-37 AACGGCC 45 8.8844576E-4 26.421465 36-37 GTACATG 4235 0.0 25.122074 1 CATGGGG 1095 0.0 25.087097 4 TTCTGCG 95 0.0012469857 24.927742 5 ACTCCCG 135 1.3796012E-5 24.54159 6 >>END_MODULE