##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939194_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 729197 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44173796655773 34.0 31.0 34.0 31.0 34.0 2 32.645320811797085 34.0 31.0 34.0 31.0 34.0 3 32.74035411555451 34.0 31.0 34.0 31.0 34.0 4 36.20980064372179 37.0 35.0 37.0 35.0 37.0 5 36.1861348853602 37.0 35.0 37.0 35.0 37.0 6 36.251690558244206 37.0 37.0 37.0 35.0 37.0 7 36.21128858182357 37.0 37.0 37.0 35.0 37.0 8 36.20439606855212 37.0 37.0 37.0 35.0 37.0 9 37.92019851974158 39.0 38.0 39.0 35.0 39.0 10-11 37.84959277122643 39.0 38.0 39.0 35.0 39.0 12-13 37.79212201915257 39.0 38.0 39.0 35.0 39.0 14-15 39.116354702501525 40.5 38.5 41.0 36.0 41.0 16-17 39.21567559932364 41.0 39.0 41.0 36.0 41.0 18-19 39.30342074912541 41.0 39.0 41.0 36.0 41.0 20-21 39.29813959739275 41.0 39.0 41.0 36.0 41.0 22-23 39.20846218511596 41.0 39.0 41.0 35.0 41.0 24-25 39.11348236484791 41.0 39.0 41.0 35.0 41.0 26-27 38.927389992004905 40.5 38.0 41.0 35.0 41.0 28-29 38.76923040001536 40.0 38.0 41.0 35.0 41.0 30-31 38.52381180942872 40.0 38.0 41.0 35.0 41.0 32-33 38.278963709395406 40.0 37.0 41.0 34.0 41.0 34-35 38.107093830610935 40.0 37.0 41.0 34.0 41.0 36-37 37.90729254234452 40.0 37.0 41.0 33.5 41.0 38-39 37.743393074848086 40.0 37.0 41.0 33.0 41.0 40-41 37.568753711274184 40.0 37.0 41.0 33.0 41.0 42-43 37.3273587247342 40.0 36.0 41.0 33.0 41.0 44-45 36.99051010906518 40.0 36.0 41.0 32.5 41.0 46-47 36.55844991134083 40.0 36.0 41.0 31.0 41.0 48-49 36.11154050277223 40.0 35.0 41.0 30.0 41.0 50-51 35.865088583743486 40.0 35.0 41.0 28.5 41.0 52-53 35.66055812078217 39.0 35.0 41.0 28.0 41.0 54-55 35.48276597407833 39.0 35.0 41.0 27.0 41.0 56-57 35.29307169393182 39.0 35.0 41.0 27.0 41.0 58-59 35.01502954619945 39.0 35.0 40.0 26.0 41.0 60-61 34.661523566333926 38.5 35.0 40.0 25.5 41.0 62-63 34.3300808972061 38.0 34.5 40.0 23.0 41.0 64-65 33.92831635346826 38.0 34.0 40.0 22.0 41.0 66-67 33.49892278766917 37.0 34.0 39.5 20.5 41.0 68-69 33.001551706877564 36.5 33.0 39.0 18.5 40.5 70-71 32.50087287797399 36.0 33.0 39.0 13.5 40.0 72-73 31.91963351467436 35.5 32.0 38.0 7.5 40.0 74-75 31.37554803434463 35.0 31.0 37.5 4.5 39.5 76-77 29.321435085443305 33.0 28.5 35.5 2.0 38.0 78-79 30.26802290738991 34.0 30.5 36.0 2.0 39.0 80-81 30.22925492013818 34.5 31.0 36.0 2.0 38.0 82-83 29.811203282514875 34.0 30.5 35.5 2.0 37.0 84-85 29.31805328326913 34.0 30.0 35.0 2.0 37.0 86-87 28.684186852112667 34.0 29.0 35.0 2.0 36.0 88-89 28.00462357908768 34.0 28.5 35.0 2.0 36.0 90-91 27.453697011918592 33.0 26.5 35.0 2.0 35.0 92-93 27.039160885192892 33.0 26.0 35.0 2.0 35.0 94-95 26.5616781199045 33.0 25.0 35.0 2.0 35.0 96-97 25.85750215648172 32.0 24.5 34.0 2.0 35.0 98-99 24.84576047350716 32.0 11.0 34.0 2.0 35.0 100-101 19.245583840855076 25.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27268078663039574 1101 2 0.2989496012073616 1101 3 0.2885533057443581 1101 4 0.15069162734306474 1101 5 0.18323975720715424 1101 6 0.1839115820359183 1101 7 0.2829709678790664 1101 8 0.23278301587262007 1101 9 0.24253602536935404 1101 10-11 0.2925816788302953 1101 12-13 0.4235880592298642 1101 14-15 0.37979964052019 1101 16-17 0.41270575348231375 1101 18-19 0.4696324809358501 1101 20-21 0.43737609250538867 1101 22-23 0.5405080513433234 1101 24-25 0.6498138027649816 1101 26-27 0.7156937860900996 1101 28-29 0.5911341888928092 1101 30-31 0.4945149291986368 1101 32-33 0.5001672604701071 1101 34-35 0.6515895565244421 1101 36-37 0.7453067072532775 1101 38-39 0.5568485889509631 1101 40-41 0.631039672188372 1101 42-43 0.666157331222081 1101 44-45 0.260506619689842 1101 46-47 0.3868208187174318 1101 48-49 0.41074136546586004 1101 50-51 0.47991694722939826 1101 52-53 0.5102363889790453 1101 54-55 0.42805225513149736 1101 56-57 0.5669223569124711 1101 58-59 0.5310437474524647 1101 60-61 0.629901051279532 1101 62-63 0.46340484089986234 1101 64-65 0.6987752988773153 1101 66-67 0.6264489928915751 1101 68-69 0.7960454948002536 1101 70-71 1.0056313498882261 1101 72-73 0.8214420645978677 1101 74-75 0.6764773509473692 1101 76-77 0.8708793251655784 1101 78-79 0.8244198935888072 1101 80-81 0.7557645171100873 1101 82-83 0.4350869199652969 1101 84-85 0.48504702673150746 1101 86-87 0.767171079232174 1101 88-89 0.7366902770287815 1101 90-91 0.9137205055408657 1101 92-93 0.9438079662797527 1101 94-95 1.0684886641835938 1101 96-97 0.6671979968866886 1101 98-99 0.47471014343914675 1101 100-101 -0.42184241392275723 1102 1 0.11478050261513317 1102 2 0.06454270496168846 1102 3 0.14762737160040018 1102 4 0.07570771414523136 1102 5 0.19530208524478354 1102 6 0.24154948476068938 1102 7 0.2144023604414116 1102 8 0.14424735864353977 1102 9 0.1606569759329446 1102 10-11 0.11893019662561954 1102 12-13 0.20544686148423352 1102 14-15 0.26531430724602245 1102 16-17 0.22296053514557457 1102 18-19 0.24324818387363223 1102 20-21 0.3418620914403334 1102 22-23 0.26637368771502423 1102 24-25 0.2685427235069682 1102 26-27 0.3642859135419556 1102 28-29 0.2640686989664829 1102 30-31 0.3319785952144443 1102 32-33 0.2602090306839955 1102 34-35 0.1863647850679797 1102 36-37 0.2029540401724219 1102 38-39 0.26134512751490746 1102 40-41 0.18608149787400663 1102 42-43 0.07187757618525126 1102 44-45 -0.16923149826525474 1102 46-47 -0.12311971558628443 1102 48-49 -0.012474663645704709 1102 50-51 -0.21304979192033358 1102 52-53 -0.16185217718763312 1102 54-55 -0.21888126754534198 1102 56-57 -0.22824688734066 1102 58-59 -0.20166294021949938 1102 60-61 -0.3569854313689049 1102 62-63 -0.34377819362574513 1102 64-65 -0.16331226879820093 1102 66-67 -0.1673195901385185 1102 68-69 -0.2767625397253539 1102 70-71 -0.3161310424966146 1102 72-73 -0.2520538193996451 1102 74-75 -0.27103390981414677 1102 76-77 -0.051884609333402665 1102 78-79 -0.19556241092881166 1102 80-81 -0.008526387744801411 1102 82-83 0.07131578523555149 1102 84-85 -0.10091890253093894 1102 86-87 -1.7077086101480177E-4 1102 88-89 0.1008816101254304 1102 90-91 0.2784873578529279 1102 92-93 -0.33215141731836795 1102 94-95 -0.2209170671557139 1102 96-97 -0.09738274776583822 1102 98-99 0.06855305858958616 1102 100-101 -1.0625520083134532 1103 1 0.06543839735197565 1103 2 0.09813520894893202 1103 3 0.1473283285381939 1103 4 0.1193181607162046 1103 5 0.04473390342660366 1103 6 0.11729709241618735 1103 7 0.1132261243967605 1103 8 0.0932605165382796 1103 9 0.07139262186596085 1103 10-11 0.07671528434492103 1103 12-13 0.12019466850177452 1103 14-15 0.14634500900041303 1103 16-17 0.1824651204725285 1103 18-19 0.2646546830761878 1103 20-21 0.16986249016441946 1103 22-23 0.314893424557134 1103 24-25 0.3917983056441301 1103 26-27 0.2977986727126023 1103 28-29 0.19037950438912787 1103 30-31 0.4237598950549497 1103 32-33 0.3508938392964396 1103 34-35 0.3995824884172663 1103 36-37 0.35157844208629285 1103 38-39 0.3011178547876412 1103 40-41 0.22490725544975732 1103 42-43 0.21501952195877294 1103 44-45 0.2671470902514912 1103 46-47 0.600165770857096 1103 48-49 0.41794798388220755 1103 50-51 0.4913461410940201 1103 52-53 0.42374889936738924 1103 54-55 0.48765780741478437 1103 56-57 0.34579119080926546 1103 58-59 0.2838334712637547 1103 60-61 0.37706560531371025 1103 62-63 0.33330513970759057 1103 64-65 0.37718314587484514 1103 66-67 0.2978169728598843 1103 68-69 0.1914641349157904 1103 70-71 0.01807449976814013 1103 72-73 0.011627400696049506 1103 74-75 -0.01607477903264609 1103 76-77 0.13277448157569083 1103 78-79 0.07858531634391497 1103 80-81 0.19325291847529513 1103 82-83 0.11734349655931808 1103 84-85 0.10933329045151652 1103 86-87 0.07700725944201281 1103 88-89 0.41133814920039313 1103 90-91 0.7656035859231025 1103 92-93 0.6853675220755768 1103 94-95 0.5948474862733129 1103 96-97 0.6570530576568068 1103 98-99 0.4344571654476397 1103 100-101 -0.2437721040072347 1104 1 0.06322836215511529 1104 2 -0.0686988846265848 1104 3 0.10149800268822418 1104 4 0.05483771741935328 1104 5 0.06521951587423302 1104 6 0.01565779694862357 1104 7 0.15134985164220183 1104 8 0.1320589122164293 1104 9 0.28254399811515185 1104 10-11 0.21855316052837992 1104 12-13 0.12016738411663397 1104 14-15 0.08349542781572694 1104 16-17 -0.02101686315187834 1104 18-19 0.2021796420876001 1104 20-21 0.2832936008125273 1104 22-23 0.13395702445890834 1104 24-25 0.19044202366248442 1104 26-27 0.3131225755702758 1104 28-29 0.2971693476527406 1104 30-31 0.2927415177813586 1104 32-33 0.2625073138140692 1104 34-35 0.34258664804580263 1104 36-37 0.24323090847069295 1104 38-39 0.15966816099285097 1104 40-41 0.13500768679108432 1104 42-43 -0.034060870241866326 1104 44-45 0.08175713437778853 1104 46-47 -0.03901559837256485 1104 48-49 -0.2827026246836013 1104 50-51 -0.14462311188431443 1104 52-53 -0.0762324976427422 1104 54-55 0.027660436637354735 1104 56-57 0.048192960821737074 1104 58-59 0.16149648926401028 1104 60-61 -0.061270444056148676 1104 62-63 0.004269097034814706 1104 64-65 -2.876412300523157E-4 1104 66-67 -0.042868262071074525 1104 68-69 0.0406422548232257 1104 70-71 0.3984175885093535 1104 72-73 0.23857271534701852 1104 74-75 0.08401054668090424 1104 76-77 -0.1907612770914433 1104 78-79 0.08181107478781158 1104 80-81 0.2993680933061924 1104 82-83 0.46814627758228156 1104 84-85 0.2957402097715871 1104 86-87 0.3638852064656568 1104 88-89 0.37291801431631555 1104 90-91 0.43615827747312963 1104 92-93 0.2261651190653602 1104 94-95 0.124949604285721 1104 96-97 -0.33339864774128003 1104 98-99 -0.3233381074086097 1104 100-101 -1.0047943755067088 1105 1 0.06907652492898109 1105 2 0.1773802423535571 1105 3 0.026432246691349803 1105 4 0.1850763979515193 1105 5 0.10811082079333545 1105 6 0.09190369690394817 1105 7 0.1420592041636226 1105 8 0.1737844069093697 1105 9 0.13998786363607252 1105 10-11 0.13132762163976963 1105 12-13 0.1309069330340762 1105 14-15 0.07435050661541709 1105 16-17 0.14366577607047049 1105 18-19 0.19398313617374185 1105 20-21 0.13284913566379686 1105 22-23 0.28069502533326585 1105 24-25 0.21789948233953282 1105 26-27 0.17150052264535987 1105 28-29 0.36894909552238175 1105 30-31 0.42742007189000475 1105 32-33 0.40581119132754395 1105 34-35 0.3485494806779741 1105 36-37 0.48176093644536166 1105 38-39 0.4832557508474409 1105 40-41 0.6308855442803392 1105 42-43 0.6005985722237241 1105 44-45 0.6130632001744019 1105 46-47 0.5786750940183722 1105 48-49 0.4889502696552839 1105 50-51 0.4592534460132143 1105 52-53 0.4679392873608279 1105 54-55 0.4344051743658852 1105 56-57 0.1905938132010192 1105 58-59 0.24889642189546635 1105 60-61 0.11012993769892176 1105 62-63 0.12884236987979847 1105 64-65 0.12477058955737164 1105 66-67 0.27002755797369815 1105 68-69 0.5544169715528398 1105 70-71 0.6334215183409597 1105 72-73 0.3129158123409965 1105 74-75 0.24626361828028465 1105 76-77 0.2970598283737722 1105 78-79 0.39634452374658835 1105 80-81 0.816291453228601 1105 82-83 0.649370043866 1105 84-85 0.3378885495670474 1105 86-87 0.44827266636107055 1105 88-89 0.4362133871486371 1105 90-91 0.6795820805467621 1105 92-93 0.6083310893515339 1105 94-95 0.3921339575835532 1105 96-97 0.2583730732382854 1105 98-99 0.08247015981038075 1105 100-101 -0.7595262988785763 1106 1 0.0331524586245564 1106 2 0.014572162934982202 1106 3 0.010186287546979145 1106 4 0.04517452482865281 1106 5 0.006991990979273055 1106 6 0.033600741634217 1106 7 0.06883087575955926 1106 8 0.0625456697197393 1106 9 0.16450124144748202 1106 10-11 0.045948067796082626 1106 12-13 0.07115041607968209 1106 14-15 0.009476718872107881 1106 16-17 -0.0263103800154596 1106 18-19 -0.049512519189995885 1106 20-21 0.06587524933507183 1106 22-23 0.08390363665454004 1106 24-25 0.21602406679055264 1106 26-27 0.06549369044689968 1106 28-29 -0.10820889333516703 1106 30-31 -0.056559798344082424 1106 32-33 -0.05524413113846549 1106 34-35 -0.14382963755573996 1106 36-37 -0.039298512851928535 1106 38-39 0.24328738988726428 1106 40-41 0.1904265489344894 1106 42-43 0.03896320069427617 1106 44-45 0.1731315221589469 1106 46-47 0.20504210688032742 1106 48-49 0.12379792080048446 1106 50-51 0.1866899959822561 1106 52-53 0.19212710403781585 1106 54-55 0.10916272302809915 1106 56-57 0.4957638157229667 1106 58-59 0.26135257431310066 1106 60-61 0.34651024812834663 1106 62-63 0.44682962255649983 1106 64-65 0.4201691726786265 1106 66-67 0.6317156612361927 1106 68-69 0.34364938093450803 1106 70-71 0.32745939348139075 1106 72-73 0.22275715860507006 1106 74-75 0.6127273824089521 1106 76-77 0.6629572995432689 1106 78-79 0.8980525733602711 1106 80-81 0.6921781487830856 1106 82-83 0.7178421992182713 1106 84-85 0.746083748686658 1106 86-87 0.6480789345592477 1106 88-89 0.6306685307852895 1106 90-91 0.8792851779629025 1106 92-93 0.8853687122967138 1106 94-95 0.5187554583745282 1106 96-97 0.34385350517996116 1106 98-99 0.4244962047011356 1106 100-101 -0.16158971451926618 1107 1 -0.013051305165184601 1107 2 -0.04437224545783636 1107 3 0.04736993452945626 1107 4 0.10844512833287467 1107 5 -0.029283155029816044 1107 6 -0.008817601433058542 1107 7 0.05583065944980348 1107 8 0.12239857908595297 1107 9 0.14979217806872214 1107 10-11 0.14666188998946694 1107 12-13 0.17145324973747478 1107 14-15 0.2212115231117764 1107 16-17 0.16880101950865622 1107 18-19 0.2116382487096331 1107 20-21 0.14848394498724815 1107 22-23 0.1811024253199136 1107 24-25 0.11881446263740258 1107 26-27 0.22196135213845025 1107 28-29 0.3891521276468808 1107 30-31 0.33898422069118084 1107 32-33 0.3035704914010111 1107 34-35 0.20317447232961428 1107 36-37 0.22498874650347034 1107 38-39 0.21896335787341314 1107 40-41 0.15482196316767016 1107 42-43 0.004829521265342862 1107 44-45 0.16642591973627674 1107 46-47 0.33172391522988676 1107 48-49 0.31000817388289903 1107 50-51 0.23224483050530154 1107 52-53 0.38491317368955436 1107 54-55 0.3585786866880696 1107 56-57 0.4766420333305845 1107 58-59 0.4377964419315319 1107 60-61 0.39839214300180004 1107 62-63 0.5651510588532815 1107 64-65 0.46708675866175753 1107 66-67 0.5117827172604166 1107 68-69 0.7217047562319294 1107 70-71 0.7410652771067419 1107 72-73 0.6047277404783102 1107 74-75 0.5554701901095775 1107 76-77 0.44136958593738385 1107 78-79 0.5427313533732452 1107 80-81 0.8424485359775495 1107 82-83 0.5373317081951292 1107 84-85 0.5599816504820296 1107 86-87 0.2954075090779611 1107 88-89 0.4355355002856136 1107 90-91 0.431545614908611 1107 92-93 0.49013834240703247 1107 94-95 0.5292140350563379 1107 96-97 0.23995992957970103 1107 98-99 0.2538386230442029 1107 100-101 -0.8194742051097919 1108 1 -0.07885492021159735 1108 2 -0.04400992661725667 1108 3 0.06746031068969671 1108 4 -0.06889976663537567 1108 5 -0.09056796206449746 1108 6 -0.17589950991701642 1108 7 -0.048496811646110416 1108 8 -0.03292221557289565 1108 9 -0.02442211927699134 1108 10-11 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-0.7790133896928282 2316 96-97 -0.878679981234125 2316 98-99 -1.0589595932322737 2316 100-101 -0.1539892552770148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 4.0 5 22.0 6 69.0 7 139.0 8 276.0 9 463.0 10 807.0 11 1342.0 12 2261.0 13 2980.0 14 3700.0 15 5018.0 16 6836.0 17 10226.0 18 9782.0 19 5569.0 20 4618.0 21 4637.0 22 4972.0 23 5310.0 24 5901.0 25 6742.0 26 7624.0 27 8619.0 28 10400.0 29 12536.0 30 15025.0 31 17924.0 32 22323.0 33 28705.0 34 40799.0 35 63705.0 36 92620.0 37 174548.0 38 144782.0 39 7911.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.1060377540409 23.25709523907908 15.675295964959401 19.96157104192062 2 14.093447998277558 24.322645320811798 40.4830244776103 21.10088220330034 3 18.36677879914481 29.791812089188518 32.26617772700656 19.575231384660114 4 10.740444626074984 19.779428604341486 41.451075635253574 28.02905113432995 5 9.882103190221573 41.40705460938539 36.386600603129196 12.324241597263839 6 24.91027801814873 41.92543304484248 18.60827732423474 14.55601161277405 7 23.142991537266337 36.843678731536194 24.14450416005551 15.868825571141954 8 23.221982536955036 35.720251180408034 21.78574514157354 19.27202114106339 9 24.573057760797152 18.845936009062022 24.596919625286446 31.98408660485438 10-11 21.979657074837117 29.283718940149235 29.14335906483433 19.593264920179323 12-13 24.367694875321757 28.34659221033548 29.201025237350127 18.084687676992637 14-15 21.16183312362648 29.546313216331765 29.710124555587175 19.581729104454585 16-17 17.13427018605857 32.701024028393576 30.849832482833435 19.31487330271442 18-19 16.95001487938102 31.48449595925381 34.00363687727733 17.561852284087838 20-21 18.164158656714164 29.868814600169774 33.79943965759596 18.167587085520097 22-23 17.748907359739547 29.996626426054963 33.76508680096051 18.48937941324498 24-25 18.340186767215698 30.71942885079673 33.64367492164321 17.296709460344363 26-27 17.813031433430247 29.855312962861376 35.213533380876626 17.11812222283175 28-29 16.559871036133607 31.064260456543607 34.95052430997681 17.425344197345982 30-31 17.53874467393585 30.280226056881748 34.18616642690521 17.99486284227719 32-33 17.369597906737074 30.817413413256922 34.36954236615233 17.443446313853674 34-35 18.22529023816365 30.243128681339105 33.993508430605154 17.538072649892094 36-37 18.063334636515155 29.70568667393705 34.50619697250286 17.724781717044934 38-39 17.15436922834246 31.177941080656613 34.13302974086051 17.534659950140423 40-41 17.24140059249506 30.827984419574285 34.428077682685974 17.50253730524468 42-43 16.99881432167748 30.92851162053905 33.419739041047514 18.652935016735963 44-45 17.521181733651872 30.263341955055502 33.19031605971754 19.025160251575087 46-47 18.614490630833014 30.96501802791481 32.5902732919425 17.830218049309675 48-49 17.94435575889233 30.82712077326487 32.685677374761376 18.54284609308143 50-51 16.927263863146873 31.08529252643856 33.46875747832699 18.51868613208757 52-53 17.512825752162996 32.010554075236186 32.075968496853385 18.400651675747433 54-55 17.33036477111124 30.98751091954575 32.211939983296695 19.470184326046322 56-57 18.131682016906293 31.00771260401867 31.65794930306804 19.202656076007 58-59 18.603195239574397 30.388923974033794 32.18258629402814 18.825294492363668 60-61 17.968438216993775 31.218860270470795 32.574848492744515 18.237853019790915 62-63 17.391211927345935 30.979804067985818 32.76187067280231 18.867113331865934 64-65 17.29620794096289 31.55066352976027 32.081679337491956 19.07144919178489 66-67 17.69301713231689 30.895083844468594 31.019539138275658 20.392359884938852 68-69 17.35940297565863 30.554433624380184 31.901688199745042 20.18447520021614 70-71 18.368131981157305 30.576937581853965 31.532511879538394 19.522418557450337 72-73 18.125897391239953 29.5345427915913 32.397555118849915 19.942004698318836 74-75 17.17375414325621 31.44842888821539 31.182314244298865 20.19550272422953 76-77 17.345332821762707 29.98940938988339 31.900317855576475 20.764939932777423 78-79 17.454227991476877 29.919466502440418 31.817898146351098 20.808407359731607 80-81 17.941502375231764 30.02617966187232 31.216812581597164 20.815505381298752 82-83 17.759261214733467 29.586586340865363 31.890147655571816 20.764004788829357 84-85 17.321382287639693 29.73380307379213 31.462074034863008 21.48274060370517 86-87 17.00711464078443 30.009644062037744 31.48165054174918 21.501590755428644 88-89 17.40049478056764 29.351655683401027 31.17158888182759 22.076260654203743 90-91 17.646200139090677 28.670720035254664 31.43621453015582 22.246865295498832 92-93 17.96613260888347 29.18196317318914 31.38205450653253 21.469849711394865 94-95 17.500358271055884 28.36526231269623 31.513727781194785 22.6206516350531 96-97 17.018058244971172 28.223618889845802 31.878671852286626 22.879651012896396 98-99 17.295910356001922 28.54755686232293 30.805593287396864 23.350939494278283 100-101 17.079280053922588 27.323131651612364 31.95221844407554 23.645369850389507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3911.0 1 2440.0 2 1226.0 3 1444.5 4 1127.5 5 1059.0 6 1954.5 7 3624.5 8 5030.0 9 5123.5 10 3887.5 11 2737.0 12 2450.5 13 2367.0 14 2263.0 15 2129.5 16 2032.0 17 1987.5 18 1993.0 19 2133.5 20 2362.0 21 2652.0 22 3050.0 23 3432.5 24 3853.5 25 4729.5 26 6388.0 27 8301.5 28 9214.0 29 10314.5 30 12573.0 31 14639.0 32 17690.0 33 22269.5 34 26874.0 35 30596.5 36 35300.5 37 39445.0 38 38655.0 39 36035.5 40 33411.5 41 30437.5 42 27651.5 43 26000.0 44 25170.0 45 24074.0 46 22807.5 47 25133.0 48 33433.0 49 30276.0 50 18948.0 51 13329.5 52 9523.0 53 7015.0 54 5302.0 55 4343.5 56 3476.5 57 2762.5 58 2202.5 59 1762.0 60 1336.0 61 948.5 62 657.5 63 407.0 64 271.0 65 202.5 66 148.5 67 129.0 68 114.5 69 101.5 70 100.0 71 84.0 72 66.5 73 60.0 74 55.0 75 42.0 76 27.5 77 19.0 78 9.0 79 4.5 80 4.0 81 2.5 82 2.5 83 2.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4363704184191652 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.171171850679584E-4 16-17 0.0024684687402718336 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.856857611866204E-5 26-27 0.005074074632780991 28-29 6.856857611866204E-5 30-31 0.0 32-33 6.856857611866204E-5 34-35 0.013370872343139098 36-37 0.0290730762743127 38-39 0.02050200425947995 40-41 0.010559560722273953 42-43 0.01076526645062994 44-45 0.004251251719357046 46-47 0.006856857611866204 48-49 0.003976977414882398 50-51 8.913914895426065E-4 52-53 0.0 54-55 0.0 56-57 1.3713715223732408E-4 58-59 0.0025370373163904956 60-61 0.003291291653695778 62-63 0.002125625859678523 64-65 0.003908408838763736 66-67 0.0026741744686278194 68-69 0.006514014731272894 70-71 2.7427430447464815E-4 72-73 0.0 74-75 0.0 76-77 0.09894445533922931 78-79 0.016662163996834876 80-81 6.856857611866204E-4 82-83 0.0 84-85 0.0 86-87 0.10552703864662087 88-89 0.413674219723888 90-91 0.41854258862831306 92-93 0.0 94-95 6.856857611866204E-5 96-97 1.3713715223732408E-4 98-99 2.0570572835598613E-4 100-101 4.799800328306343E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 729197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.350386287823717 #Duplication Level Percentage of deduplicated Percentage of total 1 70.77788554135894 21.48136166815617 2 13.31665430646235 8.08331204525087 3 5.299303551109944 4.825077294978683 4 2.7267559847314353 3.310323897969368 5 1.5984470611251873 2.4256742882893 6 1.0543275976632451 1.9199549917795604 7 0.6986027517738195 1.4842004364650434 8 0.5106339628222482 1.2398350418669957 9 0.41274452430803654 1.1274260175839659 >10 2.8821317304933953 17.96318966760368 >50 0.4120264573401056 8.686910169336217 >100 0.2879221027000241 15.82402943288535 >500 0.012184791180095064 2.464735955239476 >1k 0.008123194120063376 4.559247887860874 >5k 0.002256442811128716 4.604721204734423 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8862 1.2153094431271658 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7458 1.022768881385963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6995 0.9592743799000819 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5295 0.7261412210966309 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5009 0.6869199955567563 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3970 0.5444344943821766 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2908 0.39879483870613835 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2376 0.32583787371588196 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2173 0.2979990318117052 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2168 0.29731334605051857 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1921 0.26344046944789956 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1868 0.25617220037932137 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1834 0.25150953720325236 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1788 0.2452012282003354 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1765 0.242047073698877 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1659 0.22751053556172063 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1529 0.2096827057708685 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1516 0.2078999227917833 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1361 0.18664366419499806 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1216 0.16675877712058607 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1143 0.1567477650072614 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1065 0.14605106713275012 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1027 0.14083985534773183 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 968 0.1327487633657297 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 936 0.12836037449413534 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 930 0.1275375515807114 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 905 0.12410912277477829 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 895 0.12273775125240505 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 808 0.11080681900775785 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3713715223732408E-4 0.0 10-11 0.0 0.0 0.0 1.3713715223732408E-4 0.0 12-13 0.0 0.0 0.0 2.7427430447464815E-4 0.0 14-15 0.0 0.0 0.0 4.114114567119722E-4 0.0 16-17 0.0 0.0 0.0 4.114114567119722E-4 0.0 18-19 0.0 0.0 0.0 5.485486089492963E-4 0.0 20-21 0.0 0.0 0.0 7.542543373052824E-4 0.0 22-23 0.0 0.0 0.0 0.0011656657940172545 0.0 24-25 0.0 0.0 0.0 0.004731231752187681 0.0 26-27 0.0 0.0 0.0 0.008159660558120783 0.0 28-29 0.0 0.0 0.0 0.01899349558486938 0.0 30-31 0.0 0.0 0.0 0.04813514043530075 0.0 32-33 0.0 0.0 0.0 0.09668169232731347 0.0 34-35 0.0 0.0 0.0 0.1842437640308449 0.0 36-37 0.0 0.0 0.0 0.3086271611100978 0.0 38-39 0.0 0.0 0.0 0.48539695034400854 0.0 40-41 0.0 0.0 0.0 0.6698464201032095 0.0 42-43 0.0 0.0 0.0 0.8600556502563779 0.0 44-45 0.0 0.0 0.0 1.0896918116777772 0.0 46-47 0.0 0.0 0.0 1.363348998967357 0.0 48-49 0.0 0.0 0.0 1.6365947748002254 0.0 50-51 0.0 0.0 0.0 1.9306853977731668 0.0 52-53 0.0 0.0 0.0 2.2164792230357504 0.0 54-55 0.0 0.0 0.0 2.5342260047696303 0.0 56-57 0.0 0.0 0.0 2.9380263495324312 0.0 58-59 0.0 0.0 0.0 3.3523862550175054 0.0 60-61 0.0 0.0 0.0 3.796847765418673 0.0 62-63 0.0 0.0 0.0 4.220327291527529 0.0 64-65 0.0 0.0 0.0 4.669108622224172 0.0 66-67 0.0 0.0 0.0 5.179327397123137 0.0 68-69 0.0 0.0 0.0 5.760446079728798 0.0 70-71 0.0 0.0 0.0 6.39532252601149 0.0 72-73 0.0 0.0 0.0 7.000303073106444 0.0 74-75 0.0 0.0 0.0 7.656710052290396 0.0 76-77 0.0 0.0 0.0 8.343561479271035 1.3713715223732408E-4 78-79 0.0 0.0 0.0 9.176326836232185 1.3713715223732408E-4 80-81 0.0 0.0 0.0 10.05290751333316 1.3713715223732408E-4 82-83 0.0 0.0 0.0 10.877856052616782 1.3713715223732408E-4 84-85 0.0 0.0 0.0 11.722758047550936 1.3713715223732408E-4 86-87 0.0 0.0 0.0 12.62813752662175 1.3713715223732408E-4 88-89 0.0 0.0 0.0 13.56896695954591 1.3713715223732408E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 45 9.442343E-4 42.384884 1 GTAAGCG 110 0.0 40.98592 94-95 GATGTCG 30 8.335822E-5 39.547817 26-27 CTCCTCG 125 5.695256E-9 34.169315 9 CACGCGG 30 0.0038612897 31.816067 90-91 CTTTGCG 75 3.0935972E-4 31.638254 2 GTACATG 4275 0.0 27.996334 1 AGACGGC 120 0.0 27.683472 94-95 CGGTATA 105 6.944737E-5 27.118504 2 GACTGGT 105 6.944737E-5 27.118504 7 TCGTCCC 80 6.2987965E-8 26.694778 12-13 ACATGGG 4185 0.0 26.648708 3 TCTAGAC 90 8.9913944E-4 26.365211 3 GACTGTG 110 9.529942E-5 25.885845 7 TACATGG 4475 0.0 25.664104 2 TCCTCGT 85 1.1330667E-7 25.124496 10-11 TGGACTG 230 1.364242E-10 24.760372 5 TTGCGTT 115 1.288956E-4 24.760372 4 ACTGTGC 230 1.364242E-10 24.760372 8 CATGGGA 2665 0.0 24.75263 4 >>END_MODULE