FastQCFastQC Report
Fri 27 May 2016
SRR939193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987324
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102851.0417046481195635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.7665163613970692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.5632396254927461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36970.37444648362644883No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31020.31418257836333363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.21846931706309175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16590.16802994761598017No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16450.1666119733744951No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.13045363021662595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75300.037.763851
GGTATCA45600.036.6456381
TACCTGG8550.032.1531222
TATCAAC93750.030.1831362
ATCAACG94550.029.8776233
TCAACGC95200.029.6736264
CAACGCA96200.029.463715
AACGCAG98050.028.9004616
GTACATG77200.026.9340251
GTACCTG9900.026.8532731
TACATGG77750.026.2137622
CATGGGG22450.025.3353064
ACGCAGA111750.025.3150047
ACATGGG77850.025.2059463
CGCAGAG112350.025.179818
GCAGAGT117600.023.9739079
ATAGCCC1801.9913023E-723.699073
GAGTACT64150.022.63930112-13
CATGGGA52000.022.3318164
ATGGGAG17950.021.9166895
Produced by FastQC (version 0.11.5)