##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939193_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 987324 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67035036117829 34.0 31.0 34.0 31.0 34.0 2 32.77631355056699 34.0 31.0 34.0 31.0 34.0 3 32.82421069476686 34.0 31.0 34.0 31.0 34.0 4 36.25644874428252 37.0 37.0 37.0 35.0 37.0 5 36.29977798574733 37.0 37.0 37.0 35.0 37.0 6 36.40299334362378 37.0 37.0 37.0 35.0 37.0 7 36.3614659422844 37.0 37.0 37.0 35.0 37.0 8 36.36608651263415 37.0 37.0 37.0 35.0 37.0 9 38.14935522685562 39.0 39.0 39.0 37.0 39.0 10-11 38.085658811089374 39.0 39.0 39.0 37.0 39.0 12-13 38.01882968508818 39.0 38.0 39.0 37.0 39.0 14-15 39.43392695812114 41.0 39.0 41.0 37.0 41.0 16-17 39.3838385372988 41.0 39.0 41.0 37.0 41.0 18-19 39.35862644886582 41.0 39.0 41.0 37.0 41.0 20-21 39.28601603931435 41.0 39.0 41.0 36.5 41.0 22-23 39.198842021464074 40.5 39.0 41.0 36.0 41.0 24-25 39.224503303879985 41.0 39.0 41.0 36.0 41.0 26-27 39.13628099792976 41.0 39.0 41.0 35.5 41.0 28-29 39.00169802415418 40.5 39.0 41.0 35.0 41.0 30-31 38.785555704105235 40.0 38.0 41.0 35.0 41.0 32-33 38.52744894279892 40.0 38.0 41.0 35.0 41.0 34-35 38.21666139990519 40.0 38.0 41.0 35.0 41.0 36-37 37.96399105055686 40.0 38.0 41.0 34.5 41.0 38-39 37.73196032913208 40.0 37.5 41.0 34.0 41.0 40-41 37.55502803537643 40.0 37.0 41.0 33.5 41.0 42-43 37.312617236084606 40.0 37.0 41.0 33.0 41.0 44-45 36.98257410941089 40.0 36.0 41.0 33.0 41.0 46-47 36.518771953279774 40.0 36.0 41.0 31.5 41.0 48-49 36.36948408020062 40.0 36.0 41.0 31.0 41.0 50-51 35.3668567764989 38.5 35.0 40.0 29.0 40.5 52-53 35.53430282257901 39.0 35.0 40.0 30.0 40.5 54-55 36.0101911834413 39.0 35.0 41.0 31.0 41.0 56-57 35.897775198415104 39.0 35.0 41.0 30.0 41.0 58-59 35.707272384749075 39.0 35.0 41.0 30.0 41.0 60-61 35.43136599535715 39.0 35.0 41.0 29.0 41.0 62-63 35.0824906514984 38.0 35.0 40.5 28.0 41.0 64-65 34.72908690561559 37.5 35.0 40.0 28.0 41.0 66-67 34.35312420238949 37.0 35.0 40.0 26.5 41.0 68-69 33.965075294432225 36.5 34.5 39.0 26.0 41.0 70-71 33.571138248437194 36.0 34.0 39.0 26.0 41.0 72-73 33.137088230408665 35.5 34.0 38.5 24.5 40.0 74-75 32.69646843386771 35.0 34.0 37.5 23.5 40.0 76-77 32.27279646802873 35.0 33.0 37.0 21.5 39.0 78-79 31.865647953458037 35.0 33.0 36.5 20.0 39.0 80-81 31.45830649310662 35.0 33.0 36.0 18.0 38.5 82-83 31.056124433316725 35.0 32.0 35.5 13.0 37.0 84-85 30.705738440471414 35.0 32.0 35.0 7.0 37.0 86-87 30.34638781190369 35.0 31.5 35.0 2.0 36.0 88-89 29.986991605592493 34.0 31.0 35.0 2.0 36.0 90-91 29.655935640174857 34.0 31.0 35.0 2.0 36.0 92-93 29.303043884277095 34.0 31.0 35.0 2.0 35.0 94-95 28.9095089352634 34.0 30.0 35.0 2.0 35.0 96-97 28.419065068812266 34.0 29.5 35.0 2.0 35.0 98-99 27.809074326158385 34.0 29.0 35.0 2.0 35.0 100-101 25.30783309227771 31.5 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10968512566811484 1101 2 0.0990446769564457 1101 3 0.15503688327374476 1101 4 0.013015741974193418 1101 5 0.07809531033804973 1101 6 0.1684197344039262 1101 7 0.1470541609717273 1101 8 0.11749462594863758 1101 9 0.19887159966054924 1101 10-11 0.17107213085527206 1101 12-13 0.1663237564470066 1101 14-15 0.3044279399346337 1101 16-17 0.2314355283151457 1101 18-19 0.29334887447545555 1101 20-21 0.36565462816987093 1101 22-23 0.3055950510341816 1101 24-25 0.3137314993933771 1101 26-27 0.28647574518694086 1101 28-29 0.3680047377903861 1101 30-31 0.3833685139332914 1101 32-33 0.23781661232758466 1101 34-35 0.09075396244369216 1101 36-37 0.07427198827395642 1101 38-39 0.015043222114506705 1101 40-41 -0.01682962218229278 1101 42-43 0.10099391982921446 1101 44-45 0.11872319543579124 1101 46-47 0.10683108338083969 1101 48-49 0.20408017726288108 1101 50-51 0.043456278092897094 1101 52-53 0.0904620278259074 1101 54-55 0.11039963646233275 1101 56-57 0.10847799380999135 1101 58-59 0.20241835402718067 1101 60-61 -0.11254994373185667 1101 62-63 -0.20554833903384662 1101 64-65 -0.1817418137103033 1101 66-67 -0.25042306851817386 1101 68-69 -0.10545493447384047 1101 70-71 -0.1086497299409146 1101 72-73 -0.21450562848114885 1101 74-75 -0.27147905364498115 1101 76-77 -0.13479139192164524 1101 78-79 -0.15615642722330847 1101 80-81 -0.1664772364345879 1101 82-83 -0.13061906517554078 1101 84-85 -0.032312786691324646 1101 86-87 -0.39723020047096824 1101 88-89 -0.36447339456081806 1101 90-91 -0.5224333643198165 1101 92-93 -0.4273015834837075 1101 94-95 -0.5513329181509725 1101 96-97 -0.5799548003735211 1101 98-99 -0.8216820105040945 1101 100-101 -0.670607686182656 1102 1 0.1179654853400649 1102 2 0.16099696223470517 1102 3 0.23636819647159513 1102 4 0.21811329036412275 1102 5 0.17542741402370154 1102 6 0.23591421745784658 1102 7 0.19504335763850378 1102 8 0.1941016766606296 1102 9 0.2778413305653302 1102 10-11 0.16200896868450343 1102 12-13 0.18337139431576333 1102 14-15 0.13118210920880813 1102 16-17 0.2967589146770351 1102 18-19 0.1758335313269015 1102 20-21 0.3168831779541321 1102 22-23 0.38246724452802283 1102 24-25 0.19171257160224542 1102 26-27 0.282922941700015 1102 28-29 0.34710347191201407 1102 30-31 0.3421978234641614 1102 32-33 0.23722209963037955 1102 34-35 0.16977694148479117 1102 36-37 0.193298041434268 1102 38-39 0.24418257852569525 1102 40-41 0.14008549919051916 1102 42-43 0.24119955644636804 1102 44-45 0.14345119078070212 1102 46-47 0.18933001292861462 1102 48-49 0.23077011940356584 1102 50-51 0.27234586231826796 1102 52-53 0.22373168297561108 1102 54-55 0.31564210761501243 1102 56-57 0.279801402129884 1102 58-59 0.26354392081791644 1102 60-61 0.165045765676048 1102 62-63 0.0089884021802078 1102 64-65 0.15598915694283733 1102 66-67 0.091242225773847 1102 68-69 0.0878187829048116 1102 70-71 0.21415419614847764 1102 72-73 0.2785276488593027 1102 74-75 0.20677594454348025 1102 76-77 0.27573472162314516 1102 78-79 0.027398132903172723 1102 80-81 0.039872466583538824 1102 82-83 -0.03682219858133351 1102 84-85 0.038447399073309185 1102 86-87 -0.041662751001805276 1102 88-89 -0.04380598132234326 1102 90-91 -0.24909366316812154 1102 92-93 -0.10594962977115685 1102 94-95 -0.21888888358909142 1102 96-97 -0.31069654142289593 1102 98-99 -0.4690393658832548 1102 100-101 -0.7524396416518471 1103 1 0.1795148101855304 1103 2 0.20791775786331357 1103 3 0.2238330265151589 1103 4 0.08403944160308896 1103 5 0.1321121499910234 1103 6 0.2109329566624325 1103 7 0.1913281066417909 1103 8 0.2157674561572236 1103 9 0.24170128081138387 1103 10-11 0.2516692730743699 1103 12-13 0.2535076201856512 1103 14-15 0.24671066341128522 1103 16-17 0.18242423791473783 1103 18-19 0.2444043546569361 1103 20-21 0.35101840439975973 1103 22-23 0.327746051302384 1103 24-25 0.2528496339467594 1103 26-27 0.3365127215007817 1103 28-29 0.29620616275315825 1103 30-31 0.36483098044980977 1103 32-33 0.3390346424046484 1103 34-35 0.33906365674664585 1103 36-37 0.33225331719931006 1103 38-39 0.41317544133298867 1103 40-41 0.24475563487322205 1103 42-43 0.2586639519576508 1103 44-45 0.33405551654954735 1103 46-47 0.2319114605729098 1103 48-49 0.20703379359125762 1103 50-51 0.10821813155447302 1103 52-53 0.2177340511765351 1103 54-55 0.09815761498577302 1103 56-57 0.16489857441106182 1103 58-59 0.12115855586511515 1103 60-61 0.20639965962843831 1103 62-63 0.1486617425384793 1103 64-65 0.04063115666038897 1103 66-67 0.002462935582329351 1103 68-69 -0.08867139123399426 1103 70-71 0.0704929357875983 1103 72-73 -0.16248405092235174 1103 74-75 -0.27630336846097947 1103 76-77 -0.16893248096666724 1103 78-79 -0.11937818074222761 1103 80-81 0.0041979095348807505 1103 82-83 -0.12363957552722482 1103 84-85 -0.21750352050562682 1103 86-87 -0.21904588897666954 1103 88-89 -0.10469318760567958 1103 90-91 -0.2550587050461708 1103 92-93 -0.29416945965177277 1103 94-95 -0.38527672537588487 1103 96-97 -0.49005354228109255 1103 98-99 -0.754594969720177 1103 100-101 -0.6972323154375992 1104 1 0.04529045359437589 1104 2 -0.0016765629358630463 1104 3 0.20884306895077742 1104 4 0.1185093043967953 1104 5 0.08216395707946589 1104 6 0.09039843070279119 1104 7 0.0745388701968821 1104 8 0.07364884322326759 1104 9 0.10036032417314544 1104 10-11 0.18539854748914308 1104 12-13 0.12629371960685631 1104 14-15 0.1647410701024299 1104 16-17 0.14279016534961642 1104 18-19 0.1260713549715149 1104 20-21 0.136384716813005 1104 22-23 0.15167069673730538 1104 24-25 0.17487336621300642 1104 26-27 0.27224993306948875 1104 28-29 0.14889199180956325 1104 30-31 0.1724864402129711 1104 32-33 0.15520307373881082 1104 34-35 0.088224953881749 1104 36-37 0.16943496759056131 1104 38-39 0.1224254814807324 1104 40-41 -0.07683549170545945 1104 42-43 -0.03820771385473165 1104 44-45 0.18476255681388665 1104 46-47 0.31401524634014777 1104 48-49 0.3258188678700833 1104 50-51 0.3010471945611286 1104 52-53 0.3734830980801007 1104 54-55 0.25061509687528627 1104 56-57 0.19515017427115566 1104 58-59 0.28867144164093617 1104 60-61 0.26965050493092946 1104 62-63 0.25335165896517964 1104 64-65 0.2029770828193449 1104 66-67 0.15618314650647136 1104 68-69 0.25483985343405635 1104 70-71 0.2821771391353707 1104 72-73 0.16712287516840263 1104 74-75 0.20367885653797657 1104 76-77 0.2960960844279583 1104 78-79 0.24151043731743727 1104 80-81 0.27527052525387674 1104 82-83 0.2045946277105557 1104 84-85 0.231917472034592 1104 86-87 0.2004567849730563 1104 88-89 0.2932611473606954 1104 90-91 0.39985603874522724 1104 92-93 0.4187323752651082 1104 94-95 0.15165864885608116 1104 96-97 0.12244703518645039 1104 98-99 0.1509324396394156 1104 100-101 0.020982220905459315 1105 1 0.02803357353455027 1105 2 0.07271496185050808 1105 3 0.09683435514483563 1105 4 0.057512662842832185 1105 5 0.07178821742170527 1105 6 -0.022334272435649893 1105 7 -0.07805646947297618 1105 8 0.007975446697550126 1105 9 -0.06930387444630526 1105 10-11 0.014461424341803308 1105 12-13 0.030962694394737866 1105 14-15 0.15625094445245935 1105 16-17 0.05780030120027391 1105 18-19 0.10141249442267508 1105 20-21 0.160942002739084 1105 22-23 0.07118253342260772 1105 24-25 0.12765737534175514 1105 26-27 0.17069908308440063 1105 28-29 0.10421644708863909 1105 30-31 0.15842159670272338 1105 32-33 -0.04437701244834358 1105 34-35 -0.031612569945068 1105 36-37 -0.020939648262860544 1105 38-39 0.06021318619682603 1105 40-41 0.0626837361545114 1105 42-43 0.00684063134338686 1105 44-45 0.11949545877924805 1105 46-47 0.06008517290386095 1105 48-49 0.3194715345281125 1105 50-51 0.17486341797343385 1105 52-53 0.12209671253741305 1105 54-55 0.16767941738428505 1105 56-57 0.1772838464747153 1105 58-59 0.3082548127946083 1105 60-61 0.2558709348293178 1105 62-63 0.19242525518228604 1105 64-65 0.2412253311973842 1105 66-67 0.0882102734142407 1105 68-69 -0.024497923248738118 1105 70-71 0.044266680407083925 1105 72-73 0.03724891414712772 1105 74-75 0.09998953656926801 1105 76-77 0.0997534191963041 1105 78-79 0.09351945368607772 1105 80-81 0.18670177652355946 1105 82-83 0.12096845690093971 1105 84-85 0.09308657433104273 1105 86-87 -0.011659122477333028 1105 88-89 -0.38079345224078764 1105 90-91 -0.3734229197474903 1105 92-93 -0.1949263858702821 1105 94-95 -0.05688593808099185 1105 96-97 -0.08797344052856815 1105 98-99 -0.17748208855726588 1105 100-101 -0.1934547747333184 1106 1 0.10384379960780876 1106 2 0.1514723404370315 1106 3 0.1196750547536709 1106 4 0.10269947998235551 1106 5 0.056930415121790645 1106 6 0.1696256642749745 1106 7 0.16468056059375868 1106 8 0.12135250023017363 1106 9 0.18295332536271758 1106 10-11 0.139492737168176 1106 12-13 0.18914171169654992 1106 14-15 0.23941620552113818 1106 16-17 0.20627234232551928 1106 18-19 0.14075799747259765 1106 20-21 0.09482590655464662 1106 22-23 0.06884183274325295 1106 24-25 0.2545016205260069 1106 26-27 0.21021747575688465 1106 28-29 0.061713968825621635 1106 30-31 0.14659712409635972 1106 32-33 0.10871273385888713 1106 34-35 0.15487859502054135 1106 36-37 0.22526266792282001 1106 38-39 0.2962396367032838 1106 40-41 0.17839720827057448 1106 42-43 0.08668245072029634 1106 44-45 -0.04086207481915238 1106 46-47 -0.012854978382591753 1106 48-49 0.032878883889324584 1106 50-51 -0.05986823378083983 1106 52-53 -0.18359462114277392 1106 54-55 -0.20421204861266773 1106 56-57 -0.1862264872932471 1106 58-59 -0.1198396973443252 1106 60-61 -0.22049938938958036 1106 62-63 -0.259729281377183 1106 64-65 -0.1728592316327351 1106 66-67 -0.06162400947386715 1106 68-69 -0.024899369740730037 1106 70-71 -0.1465836581996527 1106 72-73 -0.28655140315618155 1106 74-75 -0.11431170031413274 1106 76-77 -0.16169904632747034 1106 78-79 -0.02324313192907823 1106 80-81 -0.06883711454205965 1106 82-83 0.010172971942875364 1106 84-85 0.20503073151821738 1106 86-87 0.08836582073136867 1106 88-89 0.17605190628840006 1106 90-91 0.3062821937173439 1106 92-93 0.06924306951134795 1106 94-95 0.054504222947226566 1106 96-97 0.17752774668252513 1106 98-99 0.45739108646698057 1106 100-101 0.22427652803013487 1107 1 0.15582403442027015 1107 2 0.11097685173278649 1107 3 0.11397568181210005 1107 4 0.19156319404540767 1107 5 0.19813322913410047 1107 6 0.14296559449800128 1107 7 0.0870499623507115 1107 8 0.15077307105932647 1107 9 0.17141655442696901 1107 10-11 0.14407357631803563 1107 12-13 0.17231061826208105 1107 14-15 0.23208919868995537 1107 16-17 0.21188624590758565 1107 18-19 0.16853447757894457 1107 20-21 0.17739567821874402 1107 22-23 0.25690362125065036 1107 24-25 0.26756197569631723 1107 26-27 0.2417609780682639 1107 28-29 0.24405302630562886 1107 30-31 0.3453064836813553 1107 32-33 0.3702043812197431 1107 34-35 0.4752618162704536 1107 36-37 0.48688490821806596 1107 38-39 0.6242795323138779 1107 40-41 0.4843053175609313 1107 42-43 0.5686027614253035 1107 44-45 0.6502697093776177 1107 46-47 0.7800389610435232 1107 48-49 0.8214420218570453 1107 50-51 0.7242637691325555 1107 52-53 0.7475298158742731 1107 54-55 0.6667016135317354 1107 56-57 0.7890744604079956 1107 58-59 0.7443542682003752 1107 60-61 0.7613115630045186 1107 62-63 0.6427666362664581 1107 64-65 0.5545126227232302 1107 66-67 0.5393211436171512 1107 68-69 0.493360813853819 1107 70-71 0.6136524920480539 1107 72-73 0.6985227800009355 1107 74-75 0.6627879261688392 1107 76-77 0.7731658696152763 1107 78-79 0.6553517305661245 1107 80-81 0.9142696807498005 1107 82-83 0.899085631813044 1107 84-85 0.8713206605733426 1107 86-87 0.8921324182439427 1107 88-89 0.8833294364468749 1107 90-91 1.0087113284701417 1107 92-93 1.0290108901382418 1107 94-95 1.0126359158351157 1107 96-97 1.0146109375669248 1107 98-99 1.052886373442302 1107 100-101 1.0925507264761833 1108 1 0.02990895991808884 1108 2 0.04673131754326221 1108 3 0.015454203537274225 1108 4 -0.014370233384404685 1108 5 0.042970748114562696 1108 6 0.1269726229285837 1108 7 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-0.47187601416333536 2316 56-57 -0.4880878133984723 2316 58-59 -0.40669508530321963 2316 60-61 -0.2536075848394219 2316 62-63 -0.36474206656978936 2316 64-65 -0.30696489428139273 2316 66-67 -0.37380690438079256 2316 68-69 -0.3677748060127115 2316 70-71 -0.12226856480513248 2316 72-73 -0.10011885565101153 2316 74-75 -0.22256824843331913 2316 76-77 -0.30144089854437794 2316 78-79 -0.22605708616899278 2316 80-81 -0.2670621339319261 2316 82-83 -0.28193737255040574 2316 84-85 -0.3893353006689573 2316 86-87 -0.2020898209115991 2316 88-89 -0.37715206701821913 2316 90-91 -0.4076602305005963 2316 92-93 -0.5555630318330138 2316 94-95 -0.45636975504527655 2316 96-97 -0.4611183761522746 2316 98-99 -0.40111793386006056 2316 100-101 -0.3927928149765876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2416.0 3 389.0 4 353.0 5 368.0 6 432.0 7 491.0 8 622.0 9 764.0 10 860.0 11 1380.0 12 2567.0 13 5066.0 14 4494.0 15 4918.0 16 7052.0 17 11268.0 18 10286.0 19 4807.0 20 4323.0 21 4297.0 22 4293.0 23 4400.0 24 4737.0 25 5196.0 26 5879.0 27 6725.0 28 8094.0 29 9860.0 30 12415.0 31 15850.0 32 21442.0 33 30718.0 34 48207.0 35 88631.0 36 142299.0 37 235340.0 38 235129.0 39 40948.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01991101234879 19.10807195792129 13.778054926160156 22.093962103569766 2 15.238942951540288 20.95210192141783 40.69699424274159 23.111960884300295 3 19.182842614481917 26.94233022857861 29.728094042844806 24.14673311409467 4 12.116124689270348 16.47475435629793 36.85091465021878 34.558206304212945 5 12.764570248889196 38.57143726260416 33.45149469389245 15.212497794614194 6 30.25861972855925 35.74784044367823 18.39142319398456 15.602116633777966 7 27.602791447265062 34.253308128544425 21.04755292245473 17.096347501735785 8 26.697048144776936 33.99529276297785 20.40684556329734 18.900813528947886 9 27.709363350543363 16.380947747594345 21.01721611053339 34.892472791328906 10-11 24.686846594105145 27.262412368229285 26.77247513573613 21.278265901929444 12-13 25.858582684826874 25.08904083208835 28.045936352782107 21.006440130302668 14-15 22.869908114329267 25.534040166976396 27.4657120138576 24.130339704836732 16-17 20.90406531421393 29.17450653785714 28.122743371307617 21.798684776621315 18-19 20.72302052719043 26.734710529770076 31.866381435874512 20.67588750716498 20-21 21.627514703527023 25.972885905496007 31.922112361099565 20.477487029877405 22-23 21.18510175748412 26.159816349422695 31.760114796817035 20.894967096276147 24-25 20.773585297813764 26.368774329471034 32.093636051644 20.7640043210712 26-27 20.549329888911306 26.649576358395628 31.93954552689823 20.86154822579484 28-29 20.408503614984276 26.679308846839533 32.23320006833063 20.678987469845563 30-31 20.351385133799454 26.74924653222348 32.044150728022714 20.855217605954344 32-33 20.12199757728118 26.893141881104317 31.893420645821823 21.09143989579268 34-35 20.760482030985386 27.010574071055444 31.595210564828964 20.63373333313021 36-37 20.837998769443555 26.65090334029971 31.546687413238146 20.964410477018596 38-39 20.590614249190264 26.9715907906597 31.579972170860948 20.857822789289084 40-41 20.56773204527584 26.948973954042042 31.679159421512683 20.804134579169435 42-43 20.56343570809009 27.071688090354712 31.22152137504792 21.143354826507277 44-45 20.46774824210517 27.444870821615307 31.001490314481273 21.08589062179825 46-47 20.740814943219004 27.594756611822184 30.42325526761792 21.24117317734089 48-49 20.918640296008803 27.71680427658679 30.107128254886522 21.257427172517886 50-51 20.902869730234375 27.95632656631052 29.828648080236103 21.312155623219002 52-53 20.96877289884868 28.113340815212993 29.752269099514933 21.16561718642339 54-55 20.521722329420797 28.20630937762965 29.824151966055762 21.447816326893793 56-57 20.807847786214218 28.597122302158272 29.33281413309149 21.262215778536017 58-59 20.746589546018257 27.95037306605404 29.805029784292596 21.49800760363511 60-61 20.796155544366158 28.06832668320688 30.03587664045062 21.099641131976345 62-63 20.73622769605887 28.045123140988714 30.11291969945698 21.105729463495432 64-65 20.59906104623371 27.996830827625907 30.0397061904636 21.364401935676785 66-67 20.500165029845228 28.415342232230813 29.635749380438803 21.44874335748516 68-69 20.615147828351468 28.208847728252994 29.838601388357656 21.337403055037882 70-71 20.50635357781321 28.31653710301264 29.66730494884842 21.50980437032573 72-73 20.63949925521944 28.355212268816704 29.4618814240184 21.543407051945458 74-75 20.486518513997705 28.622353099737314 29.311799574309017 21.579328811955968 76-77 20.536805253643912 28.392914801170527 29.47227116368823 21.59800878149733 78-79 20.507988250210726 28.335639705665542 29.490537433897266 21.665834610226465 80-81 20.41084329386404 28.767724321658626 29.245677273720975 21.575755110756354 82-83 20.529965984146273 28.60082471920396 29.171874126087467 21.697335170562305 84-85 20.401183894679946 28.502757624461733 29.04711325251217 22.048945228346152 86-87 20.378762069894798 28.325494363568875 29.365018025393177 21.93072554114315 88-89 20.50470106093606 28.17056544439252 29.281184416144452 22.043549078526965 90-91 20.27291152245061 28.29323493735394 29.40964955332735 22.024203986868105 92-93 20.398300408624078 28.143443071062958 29.36977300640017 22.0884835139128 94-95 20.20390861289353 27.991535254768795 29.486546374791295 22.318009757546385 96-97 20.50472111211357 27.683171317934324 29.400938017794058 22.411169552158043 98-99 20.270712160332064 27.691598067300692 29.349374405312535 22.68831536705471 100-101 20.37274900868412 27.288009934007196 29.969622359245353 22.369618698063338 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3779.0 1 2457.0 2 2143.5 3 2740.5 4 1771.0 5 1448.5 6 2463.5 7 4405.5 8 6304.5 9 6522.5 10 4783.0 11 3333.0 12 3112.5 13 3127.0 14 3133.5 15 3106.5 16 2970.0 17 2901.5 18 2983.0 19 3099.5 20 3249.5 21 3543.0 22 3924.5 23 4478.5 24 5078.0 25 5855.0 26 7164.0 27 8595.5 28 9676.0 29 10803.0 30 12272.0 31 14040.5 32 16488.0 33 18616.0 34 20431.0 35 21902.5 36 23923.0 37 26290.5 38 27030.0 39 27129.5 40 27223.0 41 28499.0 42 29879.0 43 30636.0 44 31796.5 45 32854.5 46 32705.5 47 32371.0 48 35780.5 49 36042.0 50 32894.5 51 31423.0 52 29383.0 53 28825.5 54 28460.5 55 26231.0 56 23335.0 57 20949.0 58 19488.5 59 17809.0 60 15644.0 61 13436.5 62 11025.0 63 8411.0 64 6151.0 65 4750.5 66 3640.0 67 2940.0 68 2287.0 69 1583.5 70 1205.5 71 926.0 72 758.5 73 666.0 74 555.0 75 466.5 76 349.0 77 214.5 78 138.0 79 100.0 80 71.5 81 49.0 82 42.0 83 42.0 84 27.5 85 17.5 86 17.0 87 11.0 88 5.0 89 3.5 90 2.5 91 4.5 92 3.0 93 1.0 94 1.5 95 1.0 96 1.0 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27275747373709136 2 0.09287731281727174 3 0.08912980946477549 4 0.09480170643071575 5 0.11161482957975294 6 0.06715120872175699 7 0.07484878317553306 8 0.07677317678897708 9 0.06988587333033533 10-11 0.06755634421932416 12-13 0.07069614432546965 14-15 0.07267117987610956 16-17 0.06558130866868424 18-19 0.0764186832286058 20-21 0.08366048024761881 22-23 0.08968687077393035 24-25 0.1007268130826355 26-27 0.10077745501983139 28-29 0.09556133548865418 30-31 0.08963622883673444 32-33 0.08396433187079419 34-35 0.2733145350462462 36-37 0.4080727299245233 38-39 0.38715760986261855 40-41 0.36669826723547694 42-43 0.396121232746292 44-45 0.40255275877017066 46-47 0.4080220879873274 48-49 0.4162767237502583 50-51 0.4077688783013479 52-53 0.41116188809347287 54-55 0.3997168102872006 56-57 0.3808273677131317 58-59 0.36300140582017654 60-61 0.329932220831257 62-63 0.38867686797849543 64-65 0.41546645275512395 66-67 0.422201830402178 68-69 0.4347103888895641 70-71 0.42706345637298393 72-73 0.4201761529143422 74-75 0.42706345637298393 76-77 0.40579384275070796 78-79 0.3866005485534637 80-81 0.3908544712779189 82-83 0.40098285871709793 84-85 0.40103350065429383 86-87 0.40402137494885165 88-89 0.4140991204508348 90-91 0.42756987574494293 92-93 0.4199735851655586 94-95 0.42412622401562206 96-97 0.42407558207842616 98-99 0.4192645980448161 100-101 0.408680433170874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 987324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.42863371386068 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04619376552394 41.69901499459175 2 12.271863717331344 13.11337823079426 3 3.8977053847904632 6.247472199855361 4 1.7678149985841984 3.7780776013289703 5 0.9777451144997675 2.6119792794062415 6 0.5732372666947636 1.8376370372021513 7 0.4134338221021215 1.5462442972210977 8 0.30163120675907307 1.289259461008023 9 0.21821282104085943 1.0492931598354278 >10 1.3665003273202634 13.76933542397126 >50 0.11171272465103246 4.044068099268541 >100 0.051099439656816986 4.844484300561048 >500 0.0011397644181441288 0.4183559020895745 >1k 0.0011397644181441288 1.3750329358344386 >5k 3.7992147271470963E-4 1.332507190328487 >10k+ 1.8996073635735482E-4 1.043859886703366 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10285 1.0417046481195635 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7568 0.7665163613970692 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5561 0.5632396254927461 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3697 0.37444648362644883 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3102 0.31418257836333363 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2157 0.21846931706309175 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1659 0.16802994761598017 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1645 0.1666119733744951 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.13045363021662595 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0128387439179034E-4 0.0 14-15 0.0 0.0 0.0 1.519258115876855E-4 0.0 16-17 0.0 0.0 0.0 2.0256774878358067E-4 0.0 18-19 0.0 0.0 0.0 2.0256774878358067E-4 0.0 20-21 0.0 0.0 0.0 2.0256774878358067E-4 0.0 22-23 0.0 0.0 0.0 5.570613091548469E-4 0.0 24-25 0.0 0.0 0.0 8.102709951343227E-4 0.0 26-27 0.0 0.0 0.0 0.0010634806811137985 0.0 28-29 5.064193719589517E-5 0.0 0.0 0.0020763194250317018 0.0 30-31 1.0128387439179034E-4 0.0 0.0 0.005874464714723839 0.0 32-33 1.0128387439179034E-4 0.0 0.0 0.013065619796540954 0.0 34-35 1.519258115876855E-4 0.0 0.0 0.022282452366193875 0.0 36-37 2.0256774878358067E-4 0.0 0.0 0.04405848536042879 0.0 38-39 3.03851623175371E-4 0.0 0.0 0.07677317678897708 0.0 40-41 3.03851623175371E-4 0.0 0.0 0.11353922319319695 0.0 42-43 3.03851623175371E-4 0.0 0.0 0.1520270954620773 0.0 44-45 4.0513549756716134E-4 0.0 0.0 0.2002382196725695 0.0 46-47 4.0513549756716134E-4 0.0 0.0 0.2598437797521381 0.0 48-49 4.0513549756716134E-4 0.0 0.0 0.3297296530824735 0.0 50-51 4.0513549756716134E-4 0.0 0.0 0.410149049349555 0.0 52-53 4.0513549756716134E-4 0.0 0.0 0.4920370617953175 0.0 54-55 4.0513549756716134E-4 0.0 0.0 0.5858765714193112 0.0 56-57 4.0513549756716134E-4 0.0 0.0 0.6963266364435585 0.0 58-59 4.0513549756716134E-4 0.0 0.0 0.8257674279162666 0.0 60-61 4.0513549756716134E-4 0.0 0.0 0.9761233394508793 0.0 62-63 4.0513549756716134E-4 0.0 0.0 1.1278465832897813 0.0 64-65 4.0513549756716134E-4 0.0 0.0 1.2896475726306664 0.0 66-67 4.0513549756716134E-4 0.0 0.0 1.476769530569499 0.0 68-69 4.0513549756716134E-4 0.0 0.0 1.6948843540722196 0.0 70-71 5.064193719589516E-4 0.0 0.0 1.9498158659163558 0.0 72-73 5.064193719589516E-4 0.0 0.0 2.208950658547751 0.0 74-75 5.064193719589516E-4 0.0 0.0 2.4793279612366357 0.0 76-77 5.064193719589516E-4 0.0 0.0 2.765809399953815 0.0 78-79 6.07703246350742E-4 0.0 0.0 3.101210950002228 0.0 80-81 6.07703246350742E-4 0.0 0.0 3.4764677046238113 0.0 82-83 6.07703246350742E-4 0.0 0.0 3.8728421470560828 0.0 84-85 6.07703246350742E-4 0.0 0.0 4.277471225251285 0.0 86-87 6.07703246350742E-4 0.0 0.0 4.697546094291235 0.0 88-89 6.07703246350742E-4 0.0 0.0 5.151956196750003 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7530 0.0 37.76385 1 GGTATCA 4560 0.0 36.645638 1 TACCTGG 855 0.0 32.153122 2 TATCAAC 9375 0.0 30.183136 2 ATCAACG 9455 0.0 29.877623 3 TCAACGC 9520 0.0 29.673626 4 CAACGCA 9620 0.0 29.46371 5 AACGCAG 9805 0.0 28.900461 6 GTACATG 7720 0.0 26.934025 1 GTACCTG 990 0.0 26.853273 1 TACATGG 7775 0.0 26.213762 2 CATGGGG 2245 0.0 25.335306 4 ACGCAGA 11175 0.0 25.315004 7 ACATGGG 7785 0.0 25.205946 3 CGCAGAG 11235 0.0 25.17981 8 GCAGAGT 11760 0.0 23.973907 9 ATAGCCC 180 1.9913023E-7 23.69907 3 GAGTACT 6415 0.0 22.639301 12-13 CATGGGA 5200 0.0 22.331816 4 ATGGGAG 1795 0.0 21.916689 5 >>END_MODULE