FastQCFastQC Report
Fri 27 May 2016
SRR939193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987324
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107911.0929542885618095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87900.890285255903837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65280.6611811320296073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34900.3534807216273483No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30490.30881453302056877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26330.2666804412735839No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.20337801977871497No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18240.18474178689062556No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.13126390121176026No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.10280313250766719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62250.036.9234051
GTATCAA90750.029.8373051
TACCTGG7850.024.7892252
GTACATG73850.024.5509571
TATCAAC113100.023.7940962
TACATGG74200.023.7311482
ATCAACG115050.023.3082983
TCAACGC116100.023.09754
CAACGCA116800.022.9997085
ACATGGG75650.022.6488953
AACGCAG120000.022.4654886
GAGTACT78800.021.80485212-13
AGTACTT79150.021.198712-13
CATGGGG21350.020.4521354
GTACCTG9900.020.1886861
ACGCAGA134300.020.0380657
TGCACCG2151.0867225E-619.8679375
CGCAGAG135850.019.8094378
GTACTTT86600.019.78609314-15
CATGGGA53600.019.5693424
Produced by FastQC (version 0.11.5)