##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939193_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 987324 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.790953121771576 31.0 31.0 34.0 30.0 34.0 2 32.03469276549542 33.0 31.0 34.0 30.0 34.0 3 32.090073775174105 33.0 31.0 34.0 30.0 34.0 4 35.786615133431376 37.0 35.0 37.0 35.0 37.0 5 35.714561785189055 37.0 35.0 37.0 33.0 37.0 6 35.79225158104128 37.0 35.0 37.0 35.0 37.0 7 35.73099104245415 37.0 35.0 37.0 33.0 37.0 8 35.72304937386309 37.0 35.0 37.0 33.0 37.0 9 37.330247213680614 39.0 37.0 39.0 34.0 39.0 10-11 37.20781273421896 39.0 37.0 39.0 33.5 39.0 12-13 37.08781008058145 39.0 37.0 39.0 33.0 39.0 14-15 38.14122668951631 40.0 37.0 41.0 33.0 41.0 16-17 38.02336163204784 40.0 37.0 41.0 33.0 41.0 18-19 38.08968940287079 40.0 37.5 41.0 33.5 41.0 20-21 38.20977713496279 40.0 37.5 41.0 33.0 41.0 22-23 38.110767589970465 40.0 37.0 41.0 33.0 41.0 24-25 37.99158331003804 40.0 37.0 41.0 33.0 41.0 26-27 37.73457598518824 40.0 37.0 41.0 33.0 41.0 28-29 37.45871213502356 39.0 36.0 41.0 32.0 41.0 30-31 37.08501059429326 39.0 36.0 40.0 31.5 41.0 32-33 36.76461273097787 39.0 35.0 40.0 31.0 41.0 34-35 36.56760698615652 38.0 35.0 40.0 30.5 41.0 36-37 36.337517876603826 38.0 35.0 40.0 30.0 41.0 38-39 36.084168925297064 38.0 35.0 40.0 30.0 41.0 40-41 35.75283290996674 38.0 35.0 40.0 29.5 41.0 42-43 35.42743972596635 38.0 35.0 40.0 27.5 41.0 44-45 34.992490307133224 38.0 34.0 40.0 26.5 41.0 46-47 34.431445503198546 38.0 33.5 40.0 24.0 41.0 48-49 33.85626501533438 38.0 33.0 40.0 22.5 41.0 50-51 33.41375424885853 37.0 33.0 40.0 20.0 41.0 52-53 33.03155144613116 37.0 32.0 40.0 18.5 41.0 54-55 32.66893390619492 36.5 32.0 39.5 16.5 41.0 56-57 32.25585775287545 36.0 31.0 39.0 13.5 40.0 58-59 31.809228784066832 35.5 31.0 39.0 8.5 40.0 60-61 31.292083956229163 35.0 30.0 39.0 7.5 40.0 62-63 30.720828724917048 35.0 29.0 38.0 4.5 40.0 64-65 30.135968030757887 34.5 28.0 38.0 2.0 40.0 66-67 29.654171275082952 34.0 28.0 37.5 2.0 39.5 68-69 29.02534223821157 34.0 26.5 37.0 2.0 39.0 70-71 28.39695378619379 33.5 26.0 36.0 2.0 39.0 72-73 27.75062897285997 33.0 25.5 36.0 2.0 38.5 74-75 27.09416412444142 32.5 24.5 35.0 2.0 38.0 76-77 25.442290474049045 30.5 21.5 34.0 2.0 36.0 78-79 26.37578798854277 32.0 23.5 35.0 2.0 36.5 80-81 26.34211261956561 32.0 23.5 35.0 2.0 36.0 82-83 25.91116442019033 32.0 21.5 35.0 2.0 36.0 84-85 25.321697841843203 32.0 19.5 35.0 2.0 35.5 86-87 24.46865061519825 31.0 15.5 34.0 2.0 35.0 88-89 23.641226183096936 30.5 7.0 34.0 2.0 35.0 90-91 22.856513667246013 30.0 2.0 34.0 2.0 35.0 92-93 22.22433213413226 29.0 2.0 34.0 2.0 35.0 94-95 21.507235719986546 29.0 2.0 34.0 2.0 35.0 96-97 20.493976647989918 29.0 2.0 34.0 2.0 35.0 98-99 19.22220719844752 26.5 2.0 33.5 2.0 35.0 100-101 16.016065141736654 13.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18955744968027588 1101 2 0.21175862533489465 1101 3 0.2529647098858874 1101 4 0.27052291600051603 1101 5 0.17109439454675623 1101 6 0.2398729320164108 1101 7 0.20817701266566502 1101 8 0.1376410746105421 1101 9 0.22538442378609602 1101 10-11 0.2716750469120228 1101 12-13 0.1542990478304489 1101 14-15 0.18494450419655806 1101 16-17 0.29077594638668813 1101 18-19 0.13386819926318339 1101 20-21 0.29444867393428353 1101 22-23 0.24952879905192304 1101 24-25 0.14063511451015387 1101 26-27 0.17138875642876883 1101 28-29 0.04234388520183785 1101 30-31 0.37758808179661685 1101 32-33 0.2541000467500325 1101 34-35 0.4172754787150055 1101 36-37 0.43857412247116656 1101 38-39 0.39549597350566756 1101 40-41 0.465836831591929 1101 42-43 0.8442060587277922 1101 44-45 1.0005785183795908 1101 46-47 0.8999335153626831 1101 48-49 0.48047842431829935 1101 50-51 0.6596497782122057 1101 52-53 0.7402200477927252 1101 54-55 0.4920113565187165 1101 56-57 0.561608986033356 1101 58-59 0.696626061093923 1101 60-61 0.7255101834962474 1101 62-63 0.5987628164137959 1101 64-65 0.6278862777629506 1101 66-67 0.5435117732440098 1101 68-69 0.14886486816739364 1101 70-71 0.19869408914143705 1101 72-73 -0.2089574208337801 1101 74-75 -0.33169346948110245 1101 76-77 -0.32728093824322 1101 78-79 -0.027628800529523545 1101 80-81 0.22909168622476273 1101 82-83 0.3691402151028953 1101 84-85 0.07654244318131376 1101 86-87 -0.1334953554436531 1101 88-89 0.031214648772522224 1101 90-91 -0.31509616070645663 1101 92-93 -0.5426290898369679 1101 94-95 -0.8913118072234028 1101 96-97 -0.873870134903715 1101 98-99 -1.1606872475662229 1101 100-101 -1.379024095177531 1102 1 0.14680303886816048 1102 2 0.15025428314150702 1102 3 0.1736919586589849 1102 4 0.0788922055496144 1102 5 0.14776182974322438 1102 6 0.22262931904802485 1102 7 0.14413762002378405 1102 8 0.13887072229452002 1102 9 0.2651799948586344 1102 10-11 0.24866142197652863 1102 12-13 0.14784229958983275 1102 14-15 0.11279327948941642 1102 16-17 0.19877689058044723 1102 18-19 0.09669990221543401 1102 20-21 0.24321353675171764 1102 22-23 0.30144408510970067 1102 24-25 0.01572322096251355 1102 26-27 0.1259593129541372 1102 28-29 0.16424369087359736 1102 30-31 0.16722500635992787 1102 32-33 0.15446235365692473 1102 34-35 0.04901521060789804 1102 36-37 0.237899463094557 1102 38-39 0.1731290115039812 1102 40-41 0.25603423396119496 1102 42-43 -0.03939735793613153 1102 44-45 -0.017513771070689188 1102 46-47 0.08822770857107542 1102 48-49 -0.04138350381318645 1102 50-51 -0.03122722408240719 1102 52-53 -0.15949669408006883 1102 54-55 -0.04012910877118259 1102 56-57 -0.31109448223185154 1102 58-59 -0.4818583981355715 1102 60-61 -0.3384172233744067 1102 62-63 -0.3062567810961667 1102 64-65 -0.46907583371778827 1102 66-67 -0.24811125190887395 1102 68-69 -0.4321645226080477 1102 70-71 -0.5264807513886289 1102 72-73 -0.7110922868351182 1102 74-75 -0.6593792066658715 1102 76-77 -0.5702928065391681 1102 78-79 -0.7576459277651217 1102 80-81 -0.48283699420956694 1102 82-83 -0.5869591667795611 1102 84-85 -0.5447480281470369 1102 86-87 -0.5540079648219098 1102 88-89 -0.7182111496750458 1102 90-91 -0.7261838499569215 1102 92-93 -0.7509857535393074 1102 94-95 -0.8794610776690419 1102 96-97 -0.955994131697846 1102 98-99 -1.1849749851242777 1102 100-101 -0.7926836145170331 1103 1 0.05169695867522961 1103 2 0.05320149833275423 1103 3 0.11332890693878284 1103 4 0.09180107326712061 1103 5 0.20069003414720754 1103 6 0.07945745066784582 1103 7 0.10449632642118445 1103 8 0.19807574548768514 1103 9 0.13622493635972432 1103 10-11 0.20973439080969314 1103 12-13 0.13918533023522173 1103 14-15 0.04667104555895918 1103 16-17 0.23161721083073417 1103 18-19 0.07210428881879949 1103 20-21 0.13895457723925375 1103 22-23 0.06765920031507022 1103 24-25 0.08564869867643665 1103 26-27 -7.845194033038183E-4 1103 28-29 -0.001660733751357668 1103 30-31 0.013128996452593356 1103 32-33 0.13392309909705347 1103 34-35 0.191685737369653 1103 36-37 0.11388449345749763 1103 38-39 0.07665578412409957 1103 40-41 0.21721909485655289 1103 42-43 0.18937574017039793 1103 44-45 0.08696842940077687 1103 46-47 0.15053529224569928 1103 48-49 0.15473449342753298 1103 50-51 0.0663594922657822 1103 52-53 0.05740443896285541 1103 54-55 0.06646111642516672 1103 56-57 0.10103168421574082 1103 58-59 0.3446154810427231 1103 60-61 -0.032319649150860386 1103 62-63 -0.2576119786454072 1103 64-65 -0.4017385388984991 1103 66-67 -0.09812265838123224 1103 68-69 -0.2980906738470104 1103 70-71 -0.6458348191539365 1103 72-73 -0.6145017357395552 1103 74-75 -0.6283742421345693 1103 76-77 -0.577432715070124 1103 78-79 -0.5621699519685706 1103 80-81 -0.7828804474375985 1103 82-83 -0.6321570419167095 1103 84-85 -0.840501561437641 1103 86-87 -0.8219780472744098 1103 88-89 -0.8384250481900359 1103 90-91 -0.5029526679204857 1103 92-93 -0.879148333791985 1103 94-95 -0.9633247214394771 1103 96-97 -1.4610281881653187 1103 98-99 -1.5644770111310322 1103 100-101 -1.6072268667738854 1104 1 0.1669308825287068 1104 2 0.17241169871949324 1104 3 0.06749778264796902 1104 4 0.040717744150221336 1104 5 0.1722049350097805 1104 6 0.07552517166192985 1104 7 0.09142686092514651 1104 8 0.07038612164454605 1104 9 0.13909687495677048 1104 10-11 0.13745250305178303 1104 12-13 0.07240808311650682 1104 14-15 0.14984964692156666 1104 16-17 -0.031216867894045208 1104 18-19 0.2283186095739751 1104 20-21 0.061023406971024485 1104 22-23 0.022671728610980324 1104 24-25 0.32618208070036303 1104 26-27 0.1887210803819812 1104 28-29 0.3058671331825238 1104 30-31 0.26818706218929833 1104 32-33 0.23208433243573978 1104 34-35 0.20776518370477248 1104 36-37 0.07897300548311392 1104 38-39 0.32952442290660144 1104 40-41 0.2234749932750546 1104 42-43 0.023668952891846118 1104 44-45 -0.012501180407149093 1104 46-47 0.2688897407256903 1104 48-49 0.09274472725239491 1104 50-51 -0.07366098858319248 1104 52-53 -0.10918999842976262 1104 54-55 -0.003353609987208017 1104 56-57 0.02046396212171686 1104 58-59 -0.04519051186046852 1104 60-61 -0.1506828971363241 1104 62-63 -0.42068331403635995 1104 64-65 -0.4086653996089247 1104 66-67 -0.368994535381745 1104 68-69 -0.5877797433213061 1104 70-71 -0.3293540053756665 1104 72-73 -0.38926739390956655 1104 74-75 -0.658828230620415 1104 76-77 -0.35885574909561413 1104 78-79 0.03314244147491863 1104 80-81 -0.18399490480509328 1104 82-83 -0.292904757895073 1104 84-85 -0.49258286446841026 1104 86-87 -0.6397094907672844 1104 88-89 -0.6274464551869592 1104 90-91 -0.6205492589920674 1104 92-93 -0.43947444346462206 1104 94-95 -0.5950659675814478 1104 96-97 -1.1811819155258405 1104 98-99 -1.38503309587907 1104 100-101 -1.1499765142436988 1105 1 0.14344985537154287 1105 2 0.16655062686949407 1105 3 0.16653736217224946 1105 4 0.1580602062326264 1105 5 0.23417193838551498 1105 6 0.10050172305220428 1105 7 0.2129792669470092 1105 8 0.15591411517834786 1105 9 0.18381780408362403 1105 10-11 0.13098090288408315 1105 12-13 0.14671532257759878 1105 14-15 0.2882591179987273 1105 16-17 0.2825125632448504 1105 18-19 0.2594296198308541 1105 20-21 0.13795537790772272 1105 22-23 0.11681195345828854 1105 24-25 0.1042047901926253 1105 26-27 0.25988815118598296 1105 28-29 0.16671962521864003 1105 30-31 0.1521186052158825 1105 32-33 0.3952704474009252 1105 34-35 0.31284629212162685 1105 36-37 0.4317395091981595 1105 38-39 0.5814543407392065 1105 40-41 0.4402330771022136 1105 42-43 0.5031897800118301 1105 44-45 0.8079872854702614 1105 46-47 0.6928081871094278 1105 48-49 0.6834249314389993 1105 50-51 0.5825921180874545 1105 52-53 0.6939173205621003 1105 54-55 0.7791362217447073 1105 56-57 0.6832578261500402 1105 58-59 0.8194969624757249 1105 60-61 0.9194673468588874 1105 62-63 0.9266208522521069 1105 64-65 0.5376091890138532 1105 66-67 0.7745574211183843 1105 68-69 0.6922042208552668 1105 70-71 0.5493810231732823 1105 72-73 0.4047835749394437 1105 74-75 0.2283115576721393 1105 76-77 0.6384914243203639 1105 78-79 0.9827182053376937 1105 80-81 0.6425221229917746 1105 82-83 0.6449953272547191 1105 84-85 0.5187351419026989 1105 86-87 0.43094646517958424 1105 88-89 0.8421589598210169 1105 90-91 0.8328906628603043 1105 92-93 0.7793551143183706 1105 94-95 0.21265435209766537 1105 96-97 -0.15914454470931005 1105 98-99 -0.02629854278579913 1105 100-101 -0.292092378470846 1106 1 0.04734142413879283 1106 2 0.028803094962526643 1106 3 -0.0022992565991728497 1106 4 0.008165438739730746 1106 5 -0.02017599475384202 1106 6 0.06959783683605991 1106 7 0.013450725707215838 1106 8 -0.06113140992437138 1106 9 -0.01940832697848549 1106 10-11 0.02825342189123603 1106 12-13 0.09942813739194634 1106 14-15 0.003434437710716054 1106 16-17 -0.08905506924854478 1106 18-19 -0.14371097636536234 1106 20-21 -0.06095868256831949 1106 22-23 0.021437901674907778 1106 24-25 0.07051111848807068 1106 26-27 0.10272399061594939 1106 28-29 0.16299634282083986 1106 30-31 0.24859047933938427 1106 32-33 -0.05524244063725803 1106 34-35 0.03119785647832174 1106 36-37 0.15277755347931077 1106 38-39 0.2783065720323421 1106 40-41 0.10965580775196315 1106 42-43 -0.029335950031999403 1106 44-45 0.1623730081060657 1106 46-47 0.0960028447665664 1106 48-49 0.20822736623873084 1106 50-51 0.05676358605415999 1106 52-53 -0.01260067173441115 1106 54-55 0.020168442375414486 1106 56-57 0.10530494633348297 1106 58-59 -0.14697529703641266 1106 60-61 0.011337038398110622 1106 62-63 -0.25910595859273045 1106 64-65 -0.10470840975803952 1106 66-67 -0.0210704519003464 1106 68-69 -0.3353649831673309 1106 70-71 -0.29746125224684405 1106 72-73 -0.36936316392336366 1106 74-75 -0.569790831523278 1106 76-77 -0.3020516739044936 1106 78-79 -0.2807650441736129 1106 80-81 -0.33307207134258476 1106 82-83 -0.15076368495140002 1106 84-85 -0.08495634593294099 1106 86-87 -0.013574164888009221 1106 88-89 0.27350358075917924 1106 90-91 0.09289524470293742 1106 92-93 -0.08347597198513057 1106 94-95 -0.47409641493276666 1106 96-97 -0.3336388827079837 1106 98-99 -0.5357132659161827 1106 100-101 -0.6329852168795327 1107 1 -0.14453251014854374 1107 2 -0.07740718301992544 1107 3 0.01035657899387843 1107 4 -0.06738735159596843 1107 5 0.1216097096729456 1107 6 0.06449303335188006 1107 7 0.11384908724366483 1107 8 0.15995499588202478 1107 9 0.10870758858415286 1107 10-11 0.1205088361893516 1107 12-13 0.09557192976829043 1107 14-15 0.14924091809311335 1107 16-17 0.10494869777365068 1107 18-19 0.1355121124654417 1107 20-21 0.05294596123148665 1107 22-23 0.20221886865719085 1107 24-25 0.044498173861597934 1107 26-27 -0.024323292520151085 1107 28-29 -0.06562463826123377 1107 30-31 0.11136544117015035 1107 32-33 0.0809298910730405 1107 34-35 0.22266939465074387 1107 36-37 0.23353654262629675 1107 38-39 0.18807534821728922 1107 40-41 0.21246536078437117 1107 42-43 0.10881704118324365 1107 44-45 0.10421721392052774 1107 46-47 -0.3291488235316322 1107 48-49 -0.04873971372448693 1107 50-51 0.0632804919996417 1107 52-53 0.16453006347195753 1107 54-55 -0.08726494991938694 1107 56-57 0.013295322802129306 1107 58-59 -0.04613897139072165 1107 60-61 0.154789660767932 1107 62-63 -0.2595009225894245 1107 64-65 -0.26934044558510095 1107 66-67 -0.02059373460325631 1107 68-69 -0.09093270144333587 1107 70-71 0.13185346398426745 1107 72-73 -0.20127306966286795 1107 74-75 0.1573463802432542 1107 76-77 0.05169326429213683 1107 78-79 0.14410791969142878 1107 80-81 0.239856119531197 1107 82-83 -0.0772754308833754 1107 84-85 0.017033550633211547 1107 86-87 -0.2757389498131282 1107 88-89 -0.3843482397912652 1107 90-91 -0.5753275659542965 1107 92-93 -0.8082848072872686 1107 94-95 -0.8907377770064535 1107 96-97 -0.6975307857337079 1107 98-99 -0.5964549063757829 1107 100-101 -0.7855697890761899 1108 1 0.016216091778105124 1108 2 -0.13851913999342358 1108 3 -0.034344733281599815 1108 4 0.11262565182360618 1108 5 0.004169490425411482 1108 6 0.012034934402443298 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0.009476938358179865 2316 74-75 -0.1641402005447361 2316 76-77 0.31886231588351066 2316 78-79 0.23640811540498063 2316 80-81 0.2258463579167227 2316 82-83 -0.003871819807912402 2316 84-85 0.03715234234265452 2316 86-87 0.008696408567264058 2316 88-89 0.04518084452598359 2316 90-91 0.05224317968225378 2316 92-93 0.33771028383182866 2316 94-95 0.8492720086711643 2316 96-97 0.7259243605701933 2316 98-99 0.5166368463834559 2316 100-101 0.6568180365783274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 13.0 4 16.0 5 64.0 6 138.0 7 302.0 8 616.0 9 1138.0 10 1861.0 11 2913.0 12 4511.0 13 6246.0 14 7362.0 15 9873.0 16 13161.0 17 17976.0 18 17269.0 19 12693.0 20 11900.0 21 12640.0 22 13681.0 23 14942.0 24 16737.0 25 18770.0 26 21194.0 27 24892.0 28 29035.0 29 34134.0 30 39832.0 31 46416.0 32 55316.0 33 66836.0 34 84400.0 35 111866.0 36 123424.0 37 117434.0 38 46047.0 39 1675.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.862473251183445 21.364826943916608 14.893822305593105 19.878877499306842 2 17.284295732707804 23.309875988024196 38.05589654459934 21.349931734668658 3 20.77686757335991 28.09503263366433 30.137827096272346 20.990272696703414 4 13.987910756752594 18.463138746753852 35.98099509380913 31.56795540268443 5 12.803294561866215 39.457260230684156 33.13552592664617 14.603919280803465 6 30.155551774290913 36.901868079779284 17.393074613804586 15.549505532125218 7 27.048466359573958 35.17001511155406 20.73959510758373 17.04192342128825 8 26.787457815266315 34.8882433729961 19.782968913953273 18.541329897784316 9 27.65302980581856 16.376792218157362 20.891824770794592 35.07835320522949 10-11 25.144937224254654 27.850887854442917 26.17377882032646 20.83039610097597 12-13 26.189882956354754 25.611855885200807 27.910493414522485 20.287767743921954 14-15 23.08746843065074 25.97779523260413 27.570303364544387 23.364432972200742 16-17 20.662720251349754 29.598925159739675 28.290166356142628 21.448188232767944 18-19 20.567363315799362 26.731262309187027 32.558594135750106 20.1427802392635 20-21 21.635096483018746 25.769808087314804 32.79516146675255 19.799933962913897 22-23 21.22459294010882 26.027828757327885 32.661365468681005 20.086212833882293 24-25 20.796275983190878 26.36791884301053 32.91984065972465 19.915964514073945 26-27 20.557949542773482 26.525316478132787 32.83315346777587 20.083580511317855 28-29 20.414321944974496 26.504166818592477 33.15942892100263 19.922082315430394 30-31 20.401672648490095 26.590644249219963 32.891740221909025 20.115942880380917 32-33 20.328230651741475 26.73737293937958 32.72431339661549 20.210083012263453 34-35 20.823237807373314 26.655277967983338 32.48736877504467 20.034115449598676 36-37 20.97238509408013 26.38569688189391 32.489242014776245 20.152676009249713 38-39 20.725921888786797 26.729177788966208 32.497054595590654 20.04784572665634 40-41 20.724295325944038 26.70174251464251 32.43683612339621 20.137126036017236 42-43 20.71374705555536 26.9276837812063 31.904839803472406 20.453729359765934 44-45 20.72027177314964 27.174782790131406 31.660376746946707 20.444568689772247 46-47 21.040125552825923 27.581816717790247 30.819997903401504 20.558059825982326 48-49 21.202287408919922 27.714930751794252 30.362051537599843 20.720730301685983 50-51 21.22036051018765 27.878009526770875 30.123677864285547 20.77795209875593 52-53 21.35850103841345 28.075448421920473 29.902661083221453 20.66338945644462 54-55 20.91284117473089 28.101109666127837 29.99618159793543 20.98986756120585 56-57 21.070590809096103 28.449728761784378 29.56957391899721 20.91010651012231 58-59 21.2470778418368 27.763136037857393 29.979772790807914 21.010013329497895 60-61 21.18080052023534 27.966346769876388 30.185146450401646 20.667706259486625 62-63 21.235358941427663 27.83978802623743 30.256347666141327 20.66850536619359 64-65 20.98493273615272 27.854535473298046 30.170281554047072 20.99025023650216 66-67 20.958562369149522 28.13101709627658 29.819880865925043 21.09053966864886 68-69 21.099386050695003 27.91922183457553 29.92849665728583 21.052895457443636 70-71 20.968648589520768 28.18274446888762 29.739832111849807 21.108774829741808 72-73 21.09489893895013 28.192163869206055 29.429954098148126 21.282983093695687 74-75 20.973915350989138 28.432662428949364 29.2118899165826 21.3815323034789 76-77 20.887907556305986 28.2752651424443 29.62401116987381 21.2128161313759 78-79 20.85061710076333 28.17560898534769 29.52684468081982 21.446929233069167 80-81 20.600992910459286 28.557443815174626 29.378572560808546 21.462990713557538 82-83 20.890472527355662 28.38217859788913 29.23863981076015 21.488709063995053 84-85 20.707943896836298 28.177933484854012 29.167071802164234 21.94705081614546 86-87 20.856993260247116 27.98099873233647 29.224839717353102 21.937168290063312 88-89 20.841139208025737 27.915941971546733 29.244350680794216 21.998568139633313 90-91 20.6983552153604 27.966775086090305 29.3784630454376 21.9564066531117 92-93 20.613344758154366 27.86795418727793 29.283649541589185 22.235051512978515 94-95 20.49089255401469 27.627253059785843 29.30304540353521 22.578808982664253 96-97 20.658719933881887 27.415113984872242 29.248959814609996 22.677206266635878 98-99 20.504512516085384 27.418960315888786 29.098879328113426 22.977647839912407 100-101 20.714613080392073 26.706505252869515 29.814725253021447 22.764156413716965 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3067.0 1 2060.0 2 1996.0 3 2633.0 4 1798.5 5 1448.0 6 2493.0 7 4534.5 8 6358.0 9 6517.5 10 4814.5 11 3266.0 12 3000.0 13 3035.0 14 3004.5 15 3075.0 16 3073.5 17 3040.5 18 3153.5 19 3262.5 20 3368.5 21 3691.5 22 4191.5 23 4766.0 24 5306.0 25 6199.0 26 7552.5 27 8897.0 28 9957.0 29 11027.5 30 12554.0 31 14538.5 32 16860.0 33 18755.0 34 20502.5 35 22058.0 36 24178.5 37 26412.0 38 27383.0 39 27623.0 40 27678.5 41 28706.0 42 30017.0 43 30892.0 44 31879.0 45 32883.0 46 32671.5 47 32386.0 48 34724.5 49 35144.0 50 33083.5 51 31164.0 52 28962.0 53 28235.0 54 27608.0 55 25520.0 56 22953.0 57 20692.0 58 19091.0 59 17533.5 60 15227.0 61 12948.5 62 10989.5 63 8618.0 64 6472.0 65 4973.0 66 3734.0 67 3038.5 68 2329.0 69 1633.0 70 1251.5 71 960.0 72 777.5 73 661.0 74 547.0 75 450.5 76 352.0 77 228.5 78 137.5 79 98.0 80 59.0 81 30.0 82 22.5 83 17.0 84 10.5 85 7.0 86 4.5 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2735677447322257 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013673323042891696 16-17 0.0014686161786809598 18-19 2.532096859794758E-4 20-21 0.0 22-23 0.0 24-25 3.03851623175371E-4 26-27 0.0012660484298973793 28-29 0.0 30-31 0.002785306545774234 32-33 0.0 34-35 0.008912980946477548 36-37 0.026992152525412122 38-39 0.019547787757615537 40-41 0.013166903670932744 42-43 0.008457203511714492 44-45 0.00303851623175371 46-47 0.0010634806811137985 48-49 6.07703246350742E-4 50-51 1.0128387439179032E-4 52-53 5.064193719589516E-5 54-55 0.0 56-57 0.0 58-59 0.004051354975671613 60-61 0.007950784139755541 62-63 0.0032410839805372903 64-65 0.0018231097390522262 66-67 0.0033423678549290813 68-69 0.002785306545774234 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.18393151589549123 78-79 5.570613091548468E-4 80-81 0.0024814549225988633 82-83 5.570613091548468E-4 84-85 0.0 86-87 0.08745862553731094 88-89 0.3331733048117943 90-91 0.32238657218906863 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014686161786809598 100-101 6.583451835466372E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 987324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.416683531330825 #Duplication Level Percentage of deduplicated Percentage of total 1 79.03921966631614 43.010522031452645 2 11.740837366114333 12.777948626893348 3 3.697506167802414 6.036180689653433 4 1.6830350782794532 3.663407489074464 5 0.9262269334940428 2.5201098959070167 6 0.576373148274382 1.8818589123362321 7 0.3907580212156381 1.4884628904474304 8 0.2641698447620781 1.150019747275104 9 0.21983456837584653 1.0766401322899632 >10 1.307369602773381 13.376659672926245 >50 0.10181534479327713 3.7535328382155573 >100 0.04969044670545276 4.612985447615071 >500 0.0013027457443901313 0.45653870263907165 >1k 0.0013027457443901313 1.5509960583224007 >5k 3.722130698257518E-4 1.5512998773348046 >10k+ 1.861065349128759E-4 1.0928369876171744 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10791 1.0929542885618095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8790 0.890285255903837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6528 0.6611811320296073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3490 0.3534807216273483 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3049 0.30881453302056877 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2633 0.2666804412735839 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2008 0.20337801977871497 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1824 0.18474178689062556 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1296 0.13126390121176026 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1015 0.10280313250766719 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0128387439179034E-4 0.0 14-15 1.0128387439179034E-4 0.0 0.0 1.0128387439179034E-4 0.0 16-17 2.0256774878358067E-4 0.0 0.0 1.0128387439179034E-4 0.0 18-19 2.0256774878358067E-4 0.0 0.0 1.0128387439179034E-4 0.0 20-21 2.0256774878358067E-4 0.0 0.0 1.0128387439179034E-4 0.0 22-23 2.0256774878358067E-4 0.0 0.0 4.557774347630565E-4 0.0 24-25 2.0256774878358067E-4 0.0 0.0 6.07703246350742E-4 0.0 26-27 2.0256774878358067E-4 0.0 0.0 8.609129323302177E-4 0.0 28-29 2.532096859794758E-4 0.0 0.0 0.0018737516762481212 0.0 30-31 3.03851623175371E-4 0.0 0.0 0.005671896965940258 0.0 32-33 3.03851623175371E-4 0.0 0.0 0.012559200424582001 0.0 34-35 4.0513549756716134E-4 0.0 0.0 0.021725391057039026 0.0 36-37 4.0513549756716134E-4 0.0 0.0 0.043197572428098574 0.0 38-39 4.0513549756716134E-4 0.0 0.0 0.07570969610786327 0.0 40-41 4.0513549756716134E-4 0.0 0.0 0.11136161989377347 0.0 42-43 4.557774347630565E-4 0.0 0.0 0.1486847276071482 0.0 44-45 5.064193719589516E-4 0.0 0.0 0.1960349388853102 0.0 46-47 6.07703246350742E-4 0.0 0.0 0.25422252472339374 0.0 48-49 6.07703246350742E-4 0.0 0.0 0.32147501731954253 0.0 50-51 6.07703246350742E-4 0.0 0.0 0.3979443424853442 0.0 52-53 6.07703246350742E-4 0.0 0.0 0.47664191288776536 0.0 54-55 6.07703246350742E-4 0.0 0.0 0.5675441901543972 0.0 56-57 6.07703246350742E-4 0.0 0.0 0.6731832711450345 0.0 58-59 6.07703246350742E-4 0.0 0.0 0.7961925365938638 0.0 60-61 6.07703246350742E-4 0.0 0.0 0.9387495898003086 0.0 62-63 6.07703246350742E-4 0.0 0.0 1.0819649881903004 0.0 64-65 6.07703246350742E-4 0.0 0.0 1.2350049223962953 0.0 66-67 6.07703246350742E-4 0.0 0.0 1.4111882219008147 0.0 68-69 6.07703246350742E-4 0.0 0.0 1.617452832099696 0.0 70-71 6.07703246350742E-4 0.0 0.0 1.857444972471043 0.0 72-73 6.07703246350742E-4 0.0 0.0 2.0996653580790094 0.0 74-75 6.07703246350742E-4 0.0 0.0 2.3553058570438883 0.0 76-77 6.07703246350742E-4 0.0 0.0 2.6257844436071647 0.0 78-79 6.07703246350742E-4 0.0 0.0 2.9400176639076943 0.0 80-81 6.07703246350742E-4 0.0 0.0 3.2883835498782568 0.0 82-83 6.07703246350742E-4 0.0 0.0 3.653208065437486 0.0 84-85 6.583451835466372E-4 0.0 0.0 4.024413465083397 0.0 86-87 7.089871207425323E-4 0.0 0.0 4.40473441342457 0.0 88-89 7.089871207425323E-4 0.0 0.0 4.811946230416763 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6225 0.0 36.923405 1 GTATCAA 9075 0.0 29.837305 1 TACCTGG 785 0.0 24.789225 2 GTACATG 7385 0.0 24.550957 1 TATCAAC 11310 0.0 23.794096 2 TACATGG 7420 0.0 23.731148 2 ATCAACG 11505 0.0 23.308298 3 TCAACGC 11610 0.0 23.0975 4 CAACGCA 11680 0.0 22.999708 5 ACATGGG 7565 0.0 22.648895 3 AACGCAG 12000 0.0 22.465488 6 GAGTACT 7880 0.0 21.804852 12-13 AGTACTT 7915 0.0 21.1987 12-13 CATGGGG 2135 0.0 20.452135 4 GTACCTG 990 0.0 20.188686 1 ACGCAGA 13430 0.0 20.038065 7 TGCACCG 215 1.0867225E-6 19.867937 5 CGCAGAG 13585 0.0 19.809437 8 GTACTTT 8660 0.0 19.786093 14-15 CATGGGA 5360 0.0 19.569342 4 >>END_MODULE