FastQCFastQC Report
Fri 27 May 2016
SRR939192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993984
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102541.0316061425536025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74740.7519235722104178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56580.5692244543171721No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34920.35131350202820166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.3109708003348143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.2180115575301011No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.16941922606400103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.16569683214216727No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.1266619985834782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.036.4267231
GTATCAA77100.034.2948721
TACCTGG7600.029.9318642
ATCAACG95150.027.6931363
TATCAAC96000.027.4479392
GTACATG75000.027.406591
TCAACGC96350.027.2990424
TACATGG74400.027.0721112
CAACGCA97750.026.9080585
AACGCAG99750.026.5505716
ACATGGG74600.025.601913
ATGGGAT19800.023.4567055
ACGCAGA112600.023.3943717
CGCAGAG113450.023.2190938
CATGGGA52100.023.1957594
GAGTACT63200.023.08976412-13
CATGGGG21400.022.5887684
GTACCTG10100.022.5604761
GTGTAAG4300.022.0795351
GTATAGG2806.730261E-1122.0401081
Produced by FastQC (version 0.11.5)