##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939192_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 993984 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69390855386002 34.0 31.0 34.0 31.0 34.0 2 32.79973520700534 34.0 31.0 34.0 31.0 34.0 3 32.85335578842315 34.0 31.0 34.0 31.0 34.0 4 36.27871676002833 37.0 37.0 37.0 35.0 37.0 5 36.31774555727255 37.0 37.0 37.0 35.0 37.0 6 36.42044942373318 37.0 37.0 37.0 35.0 37.0 7 36.37962180477754 37.0 37.0 37.0 35.0 37.0 8 36.38677785557916 37.0 37.0 37.0 35.0 37.0 9 38.17415572081643 39.0 39.0 39.0 37.0 39.0 10-11 38.1029327433842 39.0 39.0 39.0 37.0 39.0 12-13 38.031460767980164 39.0 38.0 39.0 37.0 39.0 14-15 39.43208391684373 41.0 39.0 41.0 37.0 41.0 16-17 39.36008175181894 41.0 39.0 41.0 37.0 41.0 18-19 39.30327500241452 40.0 39.0 41.0 37.0 41.0 20-21 39.30351746104565 40.5 39.0 41.0 36.5 41.0 22-23 39.33155865687979 41.0 39.0 41.0 36.5 41.0 24-25 39.28383756680188 41.0 39.0 41.0 36.0 41.0 26-27 39.163854750177066 41.0 39.0 41.0 36.0 41.0 28-29 39.01906922043011 40.5 39.0 41.0 35.0 41.0 30-31 38.763364400231794 40.0 38.0 41.0 35.0 41.0 32-33 38.51156356641555 40.0 38.0 41.0 35.0 41.0 34-35 38.20286946268753 40.0 38.0 41.0 35.0 41.0 36-37 37.94335975226966 40.0 38.0 41.0 34.5 41.0 38-39 37.7594674562166 40.0 37.5 41.0 34.0 41.0 40-41 37.551684936578454 40.0 37.0 41.0 33.5 41.0 42-43 37.301163298403196 40.0 37.0 41.0 33.0 41.0 44-45 36.96786668598287 40.0 36.0 41.0 33.0 41.0 46-47 36.675326262796986 40.0 36.0 41.0 31.5 41.0 48-49 36.465188071437765 40.0 36.0 41.0 31.0 41.0 50-51 35.336979770298115 38.5 35.0 40.0 29.0 40.5 52-53 35.49568705331273 38.5 35.0 40.0 30.0 40.5 54-55 35.998354098255106 39.0 35.0 41.0 31.0 41.0 56-57 35.897779541723004 39.0 35.0 41.0 30.0 41.0 58-59 35.70297208003348 39.0 35.0 41.0 30.0 41.0 60-61 35.42446206377568 39.0 35.0 41.0 29.0 41.0 62-63 35.05653662433198 38.0 35.0 40.0 28.0 41.0 64-65 34.6938884328118 37.5 35.0 40.0 28.0 41.0 66-67 34.32051018929882 37.0 35.0 40.0 26.5 41.0 68-69 33.924986720108166 36.5 34.0 39.0 26.0 41.0 70-71 33.50250305839933 36.0 34.0 39.0 25.5 41.0 72-73 33.06971440184148 35.0 34.0 38.5 24.5 40.0 74-75 32.64229253187174 35.0 33.5 37.5 23.5 39.5 76-77 32.21866347949263 35.0 33.0 37.0 21.0 39.0 78-79 31.801970152437065 35.0 33.0 36.5 19.5 39.0 80-81 31.36827453963042 35.0 32.5 36.0 17.5 38.0 82-83 30.99645768945979 35.0 32.0 35.5 10.5 37.0 84-85 30.643374038213892 35.0 32.0 35.0 6.0 37.0 86-87 30.25461526543687 35.0 31.0 35.0 2.0 36.0 88-89 29.858489673878047 34.0 31.0 35.0 2.0 36.0 90-91 29.486048065160006 34.0 31.0 35.0 2.0 36.0 92-93 29.100778785171592 34.0 30.0 35.0 2.0 35.0 94-95 28.663589655366685 34.0 29.5 35.0 2.0 35.0 96-97 28.12721733951452 34.0 29.0 35.0 2.0 35.0 98-99 27.472341104082158 34.0 28.5 35.0 2.0 35.0 100-101 24.54070940779731 31.5 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16623577907217424 1101 2 0.17962725621158881 1101 3 0.1481619348299148 1101 4 0.1778043434830252 1101 5 0.1459485166969685 1101 6 0.0692072205941372 1101 7 0.09466292122636588 1101 8 0.19649769655940474 1101 9 0.11972207928577205 1101 10-11 0.1112454589395 1101 12-13 0.14776647296981338 1101 14-15 0.25996913103971764 1101 16-17 0.28383680448233406 1101 18-19 0.30849331342736974 1101 20-21 0.2631478117871211 1101 22-23 0.28514933546374976 1101 24-25 0.23178254448747992 1101 26-27 0.22988849833765812 1101 28-29 0.1669377835610817 1101 30-31 0.15547782633475293 1101 32-33 0.29538289541596185 1101 34-35 0.24288979313664072 1101 36-37 0.3462641826199402 1101 38-39 0.32141073570764433 1101 40-41 0.325538546040228 1101 42-43 0.29288724855704373 1101 44-45 0.25123021920040145 1101 46-47 0.36590118818168094 1101 48-49 0.29265430658988123 1101 50-51 0.2725132659902201 1101 52-53 0.23803733430635532 1101 54-55 0.382950597811913 1101 56-57 0.31521922912310885 1101 58-59 0.09872711867684814 1101 60-61 0.19718807365493518 1101 62-63 0.09138110254788501 1101 64-65 -0.13995961084415853 1101 66-67 0.041619455588282506 1101 68-69 -0.07260723850053807 1101 70-71 -0.09888467661927791 1101 72-73 -0.03104093940629582 1101 74-75 -0.20398911363124483 1101 76-77 -0.11577406040000682 1101 78-79 -0.1250993710634134 1101 80-81 -0.039241550404927494 1101 82-83 -0.31096390533197393 1101 84-85 -0.15088483800182573 1101 86-87 -0.2914738135967916 1101 88-89 -0.5651224078010699 1101 90-91 -0.5273771437920836 1101 92-93 -0.5556124390857384 1101 94-95 -0.7112272912477273 1101 96-97 -1.0273200997309644 1101 98-99 -1.118713093202338 1101 100-101 -1.761135494209693 1102 1 0.10790659925271484 1102 2 0.17116101043432508 1102 3 0.15341151486350668 1102 4 0.08313422478482835 1102 5 0.21151173317811356 1102 6 0.23603541280029816 1102 7 0.21030136245875752 1102 8 0.14749777039965295 1102 9 0.27365822593250044 1102 10-11 0.1537462896422781 1102 12-13 0.23723200803470235 1102 14-15 0.28780113524860695 1102 16-17 0.2582926941650072 1102 18-19 0.1790882812145611 1102 20-21 0.1860262892012301 1102 22-23 0.14615199371262833 1102 24-25 0.13812657542654705 1102 26-27 0.17873220622563935 1102 28-29 0.2525774708476334 1102 30-31 0.22056684998208453 1102 32-33 0.25473037838656154 1102 34-35 0.20929594218317504 1102 36-37 0.21082617150698724 1102 38-39 0.2892129269169601 1102 40-41 0.3483540284939508 1102 42-43 0.45790058595158456 1102 44-45 0.3701141700228092 1102 46-47 0.5298080756306831 1102 48-49 0.6797271869133041 1102 50-51 0.5152699992977148 1102 52-53 0.5152982303577431 1102 54-55 0.4246622608788684 1102 56-57 0.4699803097751172 1102 58-59 0.5170517296310493 1102 60-61 0.5003830488261443 1102 62-63 0.377762422811486 1102 64-65 0.46421051708406935 1102 66-67 0.5618966795642066 1102 68-69 0.5838763966548015 1102 70-71 0.4971180277797558 1102 72-73 0.5164591067438593 1102 74-75 0.5519238748681303 1102 76-77 0.5415587375915436 1102 78-79 0.4150593393166808 1102 80-81 0.6262540561003966 1102 82-83 0.6193574381913152 1102 84-85 0.3034726595522166 1102 86-87 0.2489744890005383 1102 88-89 0.2841223449264021 1102 90-91 0.30396711789774855 1102 92-93 0.04044449174440956 1102 94-95 -0.06021360311200752 1102 96-97 -0.1330443340999068 1102 98-99 -0.2944332663577143 1102 100-101 -0.727507030640627 1103 1 0.10809513620596078 1103 2 0.11890934053559477 1103 3 0.15632934866369652 1103 4 0.1410509812309968 1103 5 0.13854793227313422 1103 6 0.1725613052085393 1103 7 0.18669833150278947 1103 8 0.17418023825858597 1103 9 0.19999414491547896 1103 10-11 0.22808371692087803 1103 12-13 0.26428257184996795 1103 14-15 0.2577968258325356 1103 16-17 0.23849631407451 1103 18-19 0.22559406301446217 1103 20-21 0.21295565068941613 1103 22-23 0.2884435257100364 1103 24-25 0.2875376218964121 1103 26-27 0.14523098522442268 1103 28-29 0.2522452866919238 1103 30-31 0.2882516161962698 1103 32-33 0.22350057877440577 1103 34-35 0.1792826548169515 1103 36-37 0.3318713844639589 1103 38-39 0.42786390012675923 1103 40-41 0.28144585496811914 1103 42-43 0.09202218330847955 1103 44-45 -0.07942526113427562 1103 46-47 0.03552702987905576 1103 48-49 -0.03719804471781174 1103 50-51 -0.20892288298340844 1103 52-53 -0.09490161881269188 1103 54-55 -0.10947639458331793 1103 56-57 -0.0388495042017567 1103 58-59 0.003490535922800575 1103 60-61 0.136111699260681 1103 62-63 -0.1531172367446345 1103 64-65 -0.17492975440914194 1103 66-67 0.04566005670994144 1103 68-69 0.06638286077891564 1103 70-71 0.1314012282394259 1103 72-73 0.005299514701910368 1103 74-75 -0.10116364218997376 1103 76-77 0.0032228904321627283 1103 78-79 -0.14397517192371723 1103 80-81 -0.1903443781450953 1103 82-83 -0.17889455491361872 1103 84-85 -0.18090121820401706 1103 86-87 -0.28292865687319235 1103 88-89 -0.3585866617531934 1103 90-91 -0.5598777790353822 1103 92-93 -0.5827509402745505 1103 94-95 -0.6029217119965864 1103 96-97 -0.6746840669161109 1103 98-99 -0.5308271828986904 1103 100-101 -0.30353231254825985 1104 1 0.0486627645311728 1104 2 0.15746265995253594 1104 3 0.0312423711975498 1104 4 0.038337284287251805 1104 5 0.0404150485464001 1104 6 0.05884830408452757 1104 7 0.14389872758478361 1104 8 0.08144218762431876 1104 9 0.07867619098231415 1104 10-11 0.14940813840922118 1104 12-13 0.1543307885889007 1104 14-15 0.22056489484357655 1104 16-17 0.059574745447065425 1104 18-19 0.10807189491155356 1104 20-21 0.06346926377796791 1104 22-23 0.1516435828029472 1104 24-25 0.020785538897605704 1104 26-27 0.06883577745978897 1104 28-29 0.13408639893300034 1104 30-31 0.07689748855794676 1104 32-33 0.10708012524267474 1104 34-35 0.05062848614311122 1104 36-37 0.11756782864673454 1104 38-39 -0.05387529236552524 1104 40-41 0.061386175045200275 1104 42-43 -0.06239515687600061 1104 44-45 -0.0030791175813149607 1104 46-47 0.1037641973575063 1104 48-49 0.126709875138026 1104 50-51 -0.013099808530697032 1104 52-53 0.027873765226274827 1104 54-55 -0.13014134846295633 1104 56-57 -0.06315636070231534 1104 58-59 -0.053307979661781246 1104 60-61 0.0910997454345619 1104 62-63 0.09157713782047949 1104 64-65 0.03010958616785331 1104 66-67 0.14922791697506455 1104 68-69 -0.0483504529287373 1104 70-71 -0.1414816510269361 1104 72-73 -0.25701114204486686 1104 74-75 -0.3931023922122776 1104 76-77 -0.2983413658435978 1104 78-79 -0.37834599031476657 1104 80-81 -0.28526467110303955 1104 82-83 -0.4352216080278595 1104 84-85 -0.4193075680050846 1104 86-87 -0.40954666076265056 1104 88-89 -0.46190697076851706 1104 90-91 -0.44731221140705557 1104 92-93 -0.5696730976725419 1104 94-95 -0.7348386507466067 1104 96-97 -0.7776731925026503 1104 98-99 -0.8191998568090213 1104 100-101 -0.7291163996863013 1105 1 0.016845833534418375 1105 2 0.07338264724500476 1105 3 0.15532873767114097 1105 4 0.11499524808421313 1105 5 0.12734418839413308 1105 6 0.18495825555875456 1105 7 0.12105537252783449 1105 8 0.09061040358077577 1105 9 0.24015805030904147 1105 10-11 0.17119887130718325 1105 12-13 0.15193022838360548 1105 14-15 0.21678339727881024 1105 16-17 0.25723895338524017 1105 18-19 0.30787882096400665 1105 20-21 0.21986307646267989 1105 22-23 0.2645661138390807 1105 24-25 0.1981971175176298 1105 26-27 0.21731814464035182 1105 28-29 0.161421575753387 1105 30-31 0.2820570150681405 1105 32-33 0.24689318133703608 1105 34-35 0.150981634725035 1105 36-37 0.2721114425525357 1105 38-39 0.3367263913824843 1105 40-41 0.2118580092924489 1105 42-43 0.22132846324932842 1105 44-45 0.17892607729361742 1105 46-47 0.27228553728622984 1105 48-49 0.16176968779141987 1105 50-51 -0.03511929937926084 1105 52-53 0.08923570840481432 1105 54-55 0.13310535887676167 1105 56-57 0.16671517688669724 1105 58-59 0.33236170504376616 1105 60-61 0.14262341880624518 1105 62-63 0.21116161494356334 1105 64-65 0.0669502431114708 1105 66-67 0.07049491162134558 1105 68-69 -0.04217734412496554 1105 70-71 -0.029891397346105464 1105 72-73 0.04720784810903211 1105 74-75 0.034398116760179676 1105 76-77 -0.14669497977176604 1105 78-79 -0.0902166822264654 1105 80-81 -0.07588123250749135 1105 82-83 0.00873660924856523 1105 84-85 0.11728251788262156 1105 86-87 0.035899322158247315 1105 88-89 0.0014783336864994112 1105 90-91 0.28101898536057845 1105 92-93 0.08768720337068459 1105 94-95 0.09082989296573629 1105 96-97 0.07662812814104569 1105 98-99 0.21675541178301927 1105 100-101 0.29686804253581656 1106 1 -0.05411543147435083 1106 2 0.07413423624927873 1106 3 0.0501084430352563 1106 4 0.087698705068739 1106 5 0.024906072651589284 1106 6 0.042702167308775074 1106 7 0.03671495685796344 1106 8 0.018121051994157256 1106 9 0.013140266673538292 1106 10-11 0.09847018085897474 1106 12-13 0.10997282636903805 1106 14-15 0.08868295726352216 1106 16-17 0.05709101814318984 1106 18-19 0.037395042399019474 1106 20-21 0.0959093717461883 1106 22-23 0.12772168301613362 1106 24-25 0.08865681057907437 1106 26-27 0.09191649396029078 1106 28-29 -0.016673611802723087 1106 30-31 -0.10830466818799067 1106 32-33 -0.11709249298667856 1106 34-35 -0.1459961977312645 1106 36-37 -0.10750652497319635 1106 38-39 -0.09944702424299123 1106 40-41 0.0879947335123461 1106 42-43 0.13469266876885655 1106 44-45 -0.12744986665993707 1106 46-47 -0.35839564710541083 1106 48-49 -0.23015040551824484 1106 50-51 -0.12369904524687314 1106 52-53 -0.2544332372154443 1106 54-55 -0.2738860433349046 1106 56-57 -0.28403570817975066 1106 58-59 -0.32455794965848384 1106 60-61 -0.2423645113674553 1106 62-63 -0.3061536311585229 1106 64-65 -0.18758013830370857 1106 66-67 -0.2066297081168571 1106 68-69 -0.2612991112904979 1106 70-71 -0.30131520365707587 1106 72-73 -0.11998009354426387 1106 74-75 -0.08835456172324996 1106 76-77 0.11514931567852216 1106 78-79 0.02548253921829513 1106 80-81 0.1268409492232614 1106 82-83 0.05652803138708862 1106 84-85 0.018652164300064555 1106 86-87 0.16571033109609346 1106 88-89 0.19597473114929187 1106 90-91 0.15432858696425455 1106 92-93 0.18526489915474542 1106 94-95 0.3229858812565425 1106 96-97 0.3299748110235541 1106 98-99 0.49775331404828904 1106 100-101 0.2360775961634225 1107 1 0.15832382706437897 1107 2 0.17688799028591973 1107 3 0.16526927890974008 1107 4 0.1409860737025923 1107 5 0.16155332392516186 1107 6 0.1280953210805933 1107 7 0.16527992892033438 1107 8 0.2197853495100901 1107 9 0.1587324291066068 1107 10-11 0.1673870644184774 1107 12-13 0.14733272806075348 1107 14-15 0.2282785046008442 1107 16-17 0.2134539078226183 1107 18-19 0.289385181933703 1107 20-21 0.23216341103351823 1107 22-23 0.26974583036001576 1107 24-25 0.3077568578915333 1107 26-27 0.2829571879609247 1107 28-29 0.2907424826866958 1107 30-31 0.20455606279467275 1107 32-33 0.1875266629464889 1107 34-35 0.1574136858081303 1107 36-37 0.17997695006816627 1107 38-39 0.24739717172582232 1107 40-41 0.16343639577765146 1107 42-43 0.1886505617453338 1107 44-45 0.16677007590887882 1107 46-47 0.16803899450012239 1107 48-49 0.11948331989806604 1107 50-51 -0.02744413803537782 1107 52-53 0.19872002654988563 1107 54-55 0.24565865634745876 1107 56-57 0.27129922058166756 1107 58-59 0.3873298594998147 1107 60-61 0.3646002625541769 1107 62-63 0.47602386356778936 1107 64-65 0.42776910062205786 1107 66-67 0.3831824498072436 1107 68-69 0.32966780915579363 1107 70-71 0.42826608643811426 1107 72-73 0.4947780043470047 1107 74-75 0.49725239775845154 1107 76-77 0.6040361817631137 1107 78-79 0.6513151378150148 1107 80-81 0.5856992267324905 1107 82-83 0.5397194097247571 1107 84-85 0.26930443048182084 1107 86-87 0.3185861766606273 1107 88-89 0.3604892281290226 1107 90-91 0.18052402020130742 1107 92-93 0.421825040478204 1107 94-95 0.46005968550577236 1107 96-97 0.5694250339848068 1107 98-99 0.292323601616971 1107 100-101 0.1882909615605577 1108 1 -0.08630474495863183 1108 2 0.050823015096334245 1108 3 0.06376505021704304 1108 4 0.07627719292779744 1108 5 0.09478679145317415 1108 6 0.10149772826261483 1108 7 0.07498667714407503 1108 8 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-0.11021391459190966 2316 96-97 -0.20809385940453495 2316 98-99 -0.5622826222172748 2316 100-101 -0.4332877861662787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2301.0 3 379.0 4 374.0 5 380.0 6 407.0 7 514.0 8 606.0 9 753.0 10 905.0 11 1453.0 12 2532.0 13 5489.0 14 4607.0 15 4896.0 16 7079.0 17 11938.0 18 9407.0 19 5042.0 20 4333.0 21 4275.0 22 4271.0 23 4437.0 24 5027.0 25 5361.0 26 6157.0 27 7217.0 28 8359.0 29 10203.0 30 12803.0 31 16284.0 32 22122.0 33 31563.0 34 50049.0 35 91340.0 36 144657.0 37 238201.0 38 230411.0 39 37837.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.100342751946904 18.988417487212406 13.759028021687438 22.152211739153255 2 15.292966153920515 20.925118116896453 40.64006670130623 23.141849027876795 3 19.072639885930297 27.038890237111524 29.69779785454703 24.190672022411153 4 12.175061130603975 16.517549619519897 36.780582422584686 34.52680682729144 5 12.771779359824844 38.649192756511674 33.38204355318059 15.196984330482897 6 30.322780662753747 35.6850445060249 18.392770325156196 15.59940450606516 7 27.68241920302803 34.18865797248801 20.925824327929252 17.20309849655471 8 26.751130711565622 33.95788810359563 20.369115795132075 18.921865389706674 9 27.765502648911294 16.39949387322873 20.981410813275662 34.85359266458431 10-11 24.72916956069199 27.27908268960144 26.722674864559615 21.269072885146954 12-13 25.852468480548264 25.103521310013292 28.027787550665924 21.01622265877252 14-15 22.863740409606198 25.529371619077914 27.45142645468985 24.155461516626033 16-17 20.96137343376056 29.19055084820406 28.03464789653949 21.81342782149589 18-19 20.779639142825793 26.774563424831154 31.7738235095741 20.671973922768956 20-21 21.730311097420348 25.986578018976807 31.805051589512672 20.47805929409017 22-23 21.230745998187857 26.177438840229538 31.65921675224001 20.932598409342596 24-25 20.80311887697875 26.422907072045493 31.988223661820943 20.785750389154813 26-27 20.614095809619222 26.69101636934354 31.85877508073915 20.836112740298088 28-29 20.426248043619545 26.76716089866893 32.13825273138147 20.668338326330048 30-31 20.428001461807266 26.776090793131118 31.898104382300858 20.89780336276076 32-33 20.21890491166889 26.914713261143575 31.75762200646728 21.10875982072026 34-35 20.744695608835293 27.028547186267733 31.54658042632873 20.680176778568246 36-37 20.95972036324887 26.64289875546892 31.394156752001756 21.003224129280458 38-39 20.647610271923163 27.021414069158112 31.477100449458522 20.853875209460202 40-41 20.630350206343905 27.006227944485385 31.577612936103844 20.785808913066862 42-43 20.56537023930585 27.09660993060428 31.13481723066788 21.20320259942198 44-45 20.481233791855725 27.438844173959964 30.972920621079165 21.10700141310515 46-47 20.787729920015522 27.670022592887484 30.26098018373393 21.281267303363066 48-49 20.916769651596006 27.74545359009 29.97775929718975 21.360017461124244 50-51 20.95631885648346 28.01119386772482 29.73599730813652 21.296489967655198 52-53 21.06814746253213 28.063867152765425 29.673985093689875 21.194000291012564 54-55 20.652397409931357 28.173134681245443 29.734460876413998 21.4400070324092 56-57 20.795954414429065 28.610890694536973 29.358881564492428 21.234273326541526 58-59 20.81931205988605 27.896084245315844 29.754038707552976 21.530564987245125 60-61 20.821629568002326 28.05485944375226 30.015286413266264 21.10822457497915 62-63 20.862294359873758 27.970643621771686 30.10155217632012 21.065509842034437 64-65 20.640766466194513 27.993124359261323 29.987807965092276 21.378301209451884 66-67 20.599050327968836 28.377831418813248 29.558984112675148 21.46413414054277 68-69 20.61731252646932 28.209142742782404 29.825754811477807 21.347789919270475 70-71 20.566444023426307 28.31017368598459 29.613083882613765 21.510298407975334 72-73 20.72113769172273 28.297277290882956 29.387624141337326 21.593960876056986 74-75 20.545630484159886 28.60931937675436 29.1875421615517 21.657507977534053 76-77 20.60715489163794 28.436887607486806 29.41823121718435 21.5377262836909 78-79 20.6329580013001 28.344260945416487 29.449144302564793 21.573636750718617 80-81 20.450157750696317 28.73765094413009 29.22676397404463 21.585427331128965 82-83 20.543858080401638 28.566441709392382 29.069501340498828 21.820198869707152 84-85 20.493606514066276 28.34890808363133 29.11633856050208 22.04114684180031 86-87 20.443134626099795 28.267424538247777 29.363516181976067 21.92592465367636 88-89 20.56488035937655 28.06911895759785 29.233142099636865 22.13285858338874 90-91 20.30930017639215 28.226331695057333 29.42767795472012 22.036690173830397 92-93 20.37269666929765 28.1193563155198 29.409666181905646 22.098280833276906 94-95 20.264979834206965 27.9337413988421 29.413711526587417 22.38756724036352 96-97 20.51212476295433 27.686277601009373 29.351471338001232 22.450126298035066 98-99 20.27338535187298 27.670514664195434 29.27563796147421 22.780462022457375 100-101 20.44818351699481 27.158508621303724 29.967965962382614 22.425341899318855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3658.0 1 2345.0 2 2084.0 3 2720.5 4 1750.5 5 1439.0 6 2517.5 7 4495.5 8 6292.5 9 6417.0 10 4726.5 11 3368.5 12 3091.5 13 3060.0 14 3090.5 15 3042.0 16 2998.0 17 3022.5 18 3007.0 19 3037.5 20 3176.0 21 3423.0 22 3866.0 23 4527.0 24 5158.5 25 5920.0 26 7232.0 27 8648.5 28 9635.5 29 10700.0 30 12255.0 31 14105.5 32 16568.5 33 18633.5 34 20386.0 35 21890.0 36 24007.0 37 26552.5 38 27461.5 39 27306.0 40 27206.5 41 28777.0 42 30321.5 43 30926.0 44 32220.5 45 33391.0 46 32822.0 47 32488.0 48 36160.5 49 36243.5 50 32997.5 51 31572.0 52 29819.0 53 29350.0 54 28609.0 55 26402.5 56 23673.5 57 21281.5 58 19637.0 59 17885.0 60 15574.5 61 13300.0 62 11178.0 63 8548.5 64 6236.0 65 4897.5 66 3753.0 67 3068.0 68 2379.5 69 1641.5 70 1247.0 71 965.5 72 815.5 73 687.5 74 542.5 75 467.0 76 361.0 77 227.5 78 140.5 79 95.0 80 69.5 81 54.0 82 40.0 83 29.0 84 25.5 85 18.5 86 14.5 87 10.5 88 6.5 89 4.5 90 3.0 91 4.0 92 2.0 93 1.5 94 1.5 95 0.5 96 0.5 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3199246667954414 2 0.09054471701757776 3 0.09124895370549224 4 0.10342218788229991 5 0.10432763505247569 6 0.052616541111325736 7 0.06026253943725452 8 0.058652855579164255 9 0.05603711930976756 10-11 0.0561377245508982 12-13 0.05930678964651343 14-15 0.054528040692807936 16-17 0.05648984289485545 18-19 0.0630794861889125 20-21 0.06172131543364883 22-23 0.06881398493335909 24-25 0.0810878243512974 26-27 0.08214417938316915 28-29 0.07640968063872255 30-31 0.06971943210353486 32-33 0.06800914300431395 34-35 0.28174497778636276 36-37 0.4441218369712188 38-39 0.4263147092910953 40-41 0.4034773195544395 42-43 0.4251074463975275 44-45 0.43456433906380787 46-47 0.4317976949327152 48-49 0.43833703560620696 50-51 0.4361237203013328 52-53 0.4362243255424635 54-55 0.43064073465971286 56-57 0.40901060781662485 58-59 0.3994531099092138 60-61 0.3583558689073466 62-63 0.41731054020990277 64-65 0.44301517931878176 66-67 0.45116420385036377 68-69 0.4659028716760028 70-71 0.46112412272229736 72-73 0.446335152276093 74-75 0.45136541433262506 76-77 0.4295340770072758 78-79 0.4085075816109716 80-81 0.4156002511106819 82-83 0.42661652501448716 84-85 0.42128444723456315 86-87 0.4219886839224776 88-89 0.4295843796278411 90-91 0.4454297051059172 92-93 0.45201934839997426 94-95 0.44638545489665826 96-97 0.44864907282209776 98-99 0.44920240164831626 100-101 0.42812560363144675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 993984.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.39743837216645 #Duplication Level Percentage of deduplicated Percentage of total 1 77.78069732824943 41.53289992129331 2 12.357394706698873 13.19706444582978 3 4.013531778822245 6.4293694744317715 4 1.7880565456938626 3.819105568185474 5 0.9941336591609324 2.6542095399371104 6 0.6225328221578842 1.9944994803495886 7 0.42458768160820126 1.587032619357847 8 0.29818146554508534 1.2737701144132774 9 0.21128551706562798 1.0153894838798871 >10 1.345584489339206 13.574972975805414 >50 0.1098516408830493 3.937234604704269 >100 0.05095414118124003 4.769598472006519 >500 0.0015097522797309889 0.5139327650028546 >1k 0.0011323142097982414 1.3442868794388392 >5k 3.774380699327472E-4 1.3233264843171544 >10k+ 1.887190349663736E-4 1.033307171046916 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10254 1.0316061425536025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7474 0.7519235722104178 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5658 0.5692244543171721 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3492 0.35131350202820166 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3091 0.3109708003348143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2167 0.2180115575301011 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1684 0.16941922606400103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1647 0.16569683214216727 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1259 0.1266619985834782 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.030262056532097E-5 0.0 12-13 0.0 0.0 0.0 1.0060524113064195E-4 0.0 14-15 0.0 0.0 0.0 1.0060524113064195E-4 0.0 16-17 0.0 0.0 0.0 1.0060524113064195E-4 0.0 18-19 1.0060524113064195E-4 0.0 0.0 1.0060524113064195E-4 0.0 20-21 2.012104822612839E-4 0.0 0.0 3.018157233919258E-4 0.0 22-23 2.012104822612839E-4 0.0 0.0 6.036314467838516E-4 0.0 24-25 3.018157233919258E-4 0.0 0.0 7.042366879144936E-4 0.0 26-27 3.018157233919258E-4 0.0 0.0 0.0013078681346983452 0.0 28-29 3.521183439572468E-4 0.0 0.0 0.003370275577876505 0.0 30-31 4.024209645225678E-4 0.0 0.0 0.0076963009464941085 0.0 32-33 5.030262056532097E-4 0.0 0.0 0.01564411499581482 0.0 34-35 6.036314467838516E-4 0.0 0.0 0.026911902002446718 0.0 36-37 7.042366879144936E-4 0.0 0.0 0.044970542785396944 0.0 38-39 7.042366879144936E-4 0.0 0.0 0.08018237718112162 0.0 40-41 7.042366879144936E-4 0.0 0.0 0.1189656976369841 0.0 42-43 7.042366879144936E-4 0.0 0.0 0.15543509754684182 0.0 44-45 7.042366879144936E-4 0.0 0.0 0.20669346790290388 0.0 46-47 7.042366879144936E-4 0.0 0.0 0.27223778249951713 0.0 48-49 7.042366879144936E-4 0.0 0.0 0.3452268849397978 0.0 50-51 7.042366879144936E-4 0.0 0.0 0.43179769493271525 0.0 52-53 7.042366879144936E-4 0.0 0.0 0.5181672944433713 0.0 54-55 7.042366879144936E-4 0.0 0.0 0.615754378340094 0.0 56-57 7.042366879144936E-4 0.0 0.0 0.7277783143390638 0.0 58-59 7.042366879144936E-4 0.0 0.0 0.860074206425858 0.0 60-61 7.042366879144936E-4 0.0 0.0 1.0184268559654883 0.0 62-63 7.042366879144936E-4 0.0 0.0 1.1803509915652566 0.0 64-65 7.042366879144936E-4 0.0 0.0 1.342124219303329 0.0 66-67 7.042366879144936E-4 0.0 0.0 1.525980297469577 0.0 68-69 7.042366879144936E-4 0.0 0.0 1.7450482100315499 0.0 70-71 7.042366879144936E-4 0.0 0.0 2.0004346146416845 0.0 72-73 7.042366879144936E-4 0.0 0.0 2.260096741999871 0.0 74-75 7.042366879144936E-4 0.0 0.0 2.5243364078295025 0.0 76-77 7.042366879144936E-4 0.0 0.0 2.8298745251432615 0.0 78-79 7.042366879144936E-4 0.0 0.0 3.169014792994656 0.0 80-81 7.042366879144936E-4 0.0 0.0 3.5460329341317367 0.0 82-83 7.042366879144936E-4 0.0 0.0 3.945284833558689 0.0 84-85 7.545393084798146E-4 0.0 0.0 4.344687640847338 0.0 86-87 8.048419290451356E-4 0.0 0.0 4.776284125297792 0.0 88-89 8.048419290451356E-4 0.0 0.0 5.245054246346018 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4900 0.0 36.426723 1 GTATCAA 7710 0.0 34.294872 1 TACCTGG 760 0.0 29.931864 2 ATCAACG 9515 0.0 27.693136 3 TATCAAC 9600 0.0 27.447939 2 GTACATG 7500 0.0 27.40659 1 TCAACGC 9635 0.0 27.299042 4 TACATGG 7440 0.0 27.072111 2 CAACGCA 9775 0.0 26.908058 5 AACGCAG 9975 0.0 26.550571 6 ACATGGG 7460 0.0 25.60191 3 ATGGGAT 1980 0.0 23.456705 5 ACGCAGA 11260 0.0 23.394371 7 CGCAGAG 11345 0.0 23.219093 8 CATGGGA 5210 0.0 23.195759 4 GAGTACT 6320 0.0 23.089764 12-13 CATGGGG 2140 0.0 22.588768 4 GTACCTG 1010 0.0 22.560476 1 GTGTAAG 430 0.0 22.079535 1 GTATAGG 280 6.730261E-11 22.040108 1 >>END_MODULE