FastQCFastQC Report
Fri 27 May 2016
SRR939192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993984
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106801.074463975275256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.8740583349430172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63070.6345172558109587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34430.3463838452128002No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29960.3014133024274032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25230.25382702337260965No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.19527477303457602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.1686143841349559No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.13400618118601507No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.10432763505247569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG654.447429E-851.1115385
GGTATCA62700.038.111961
GTATCAA92250.030.4962371
ATAACGC804.5150347E-429.6629453
TATCAAC114400.024.4356292
ATCAACG115850.024.0888213
GTACATG76250.023.9726771
TACATGG75300.023.9509582
TCAACGC117350.023.8213544
GTATAGG2602.3646862E-1123.8009971
TACCTGG7800.023.7303562
CAACGCA118650.023.5603545
AACGCAG121600.023.0278116
GAGTACT77400.022.81172212-13
ACATGGG77000.022.4359723
ATGGGAT20850.021.6247845
AGTACTT79700.021.02192512-13
GTACCTG9150.020.8096141
ATGCACT6850.020.7857134
ACGCAGA135650.020.5727237
Produced by FastQC (version 0.11.5)