FastQCFastQC Report
Fri 27 May 2016
SRR939191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476552
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95100.27354689358882023No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77620.22326719117102234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74220.2134873863529152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.13404085427170367No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36170.10403986478556915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG141350.026.4329911
TACATGG138750.025.9824312
ACATGGG138800.024.8467753
CATGGGG53950.021.7765144
AGTACAT103150.020.8504142
GTATCAA205500.020.514861
GAGTACT93000.019.95979712-13
GAGTACA124900.019.6895031
CATGGGA90100.019.5064034
GTGTAGC24750.018.4146481
ATCAACG227700.018.3290943
CAACGCA227300.018.3196645
TCAACGC228250.018.2849274
GTACTTT103300.018.01636114-15
AACGCAG232550.017.8991246
TATCAAC234700.017.8833432
AGTACTT98700.017.7995812-13
GGTATCA162700.017.62451
ATGGGGA34600.017.1144125
AGAGTAC176200.016.6487710-11
Produced by FastQC (version 0.11.5)