##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939191_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3476552 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24140182571698 33.0 31.0 34.0 31.0 34.0 2 32.328801064963216 34.0 31.0 34.0 31.0 34.0 3 32.40448870029846 34.0 31.0 34.0 30.0 34.0 4 35.82985038049193 37.0 35.0 37.0 35.0 37.0 5 35.88288971371635 37.0 35.0 37.0 35.0 37.0 6 35.9701451323035 37.0 37.0 37.0 35.0 37.0 7 35.902181529285336 37.0 37.0 37.0 35.0 37.0 8 35.89815483847214 37.0 37.0 37.0 35.0 37.0 9 37.61151364915583 39.0 38.0 39.0 35.0 39.0 10-11 37.531254961812735 39.0 38.0 39.0 35.0 39.0 12-13 37.45566339867777 39.0 38.0 39.0 35.0 39.0 14-15 38.813814808465395 40.0 38.0 41.0 35.0 41.0 16-17 38.760611807330946 40.0 38.0 41.0 35.0 41.0 18-19 38.70266071095729 40.0 38.0 41.0 34.5 41.0 20-21 38.61020444969614 40.0 38.0 41.0 34.0 41.0 22-23 38.53766663061562 40.0 38.0 41.0 34.0 41.0 24-25 38.59017670381458 40.0 38.0 41.0 34.0 41.0 26-27 38.52830261132294 40.0 38.0 41.0 34.0 41.0 28-29 38.42147334485433 40.0 38.0 41.0 34.0 41.0 30-31 38.28755574488746 40.0 38.0 41.0 34.0 41.0 32-33 38.120831933478925 40.0 38.0 41.0 34.0 41.0 34-35 37.832347107133735 40.0 38.0 41.0 33.0 41.0 36-37 37.60920130059898 40.0 38.0 41.0 33.0 41.0 38-39 37.40465783339354 40.0 37.5 41.0 32.5 41.0 40-41 37.29063422609528 40.0 37.0 41.0 32.0 41.0 42-43 37.124640735993594 40.0 37.0 41.0 31.0 41.0 44-45 36.90331124056249 40.0 37.0 41.0 31.0 41.0 46-47 36.59790289344154 40.0 36.5 41.0 30.0 41.0 48-49 36.631344216913774 40.0 37.0 41.0 30.0 41.0 50-51 35.658368981680695 39.0 35.5 40.0 29.0 40.5 52-53 35.83804556928819 39.0 35.5 40.0 29.5 40.5 54-55 36.38049222332932 40.0 36.0 41.0 30.0 41.0 56-57 36.2985479578617 40.0 36.0 41.0 30.0 41.0 58-59 36.14595610823598 39.0 36.0 41.0 29.0 41.0 60-61 35.892540942865224 39.0 35.0 41.0 28.5 41.0 62-63 35.563861118717625 39.0 35.0 41.0 28.0 41.0 64-65 35.22346882773507 38.0 35.0 40.0 28.0 41.0 66-67 34.85055839809098 38.0 35.0 40.0 27.5 41.0 68-69 34.46060349449685 37.0 34.0 40.0 26.0 41.0 70-71 34.06930343627825 36.5 34.0 39.0 26.0 41.0 72-73 33.61251722971496 36.0 34.0 39.0 26.0 40.5 74-75 33.16135771879725 35.5 33.5 38.5 25.0 40.0 76-77 32.71499505832216 35.0 33.0 37.0 24.0 39.5 78-79 32.28539829118046 35.0 33.0 37.0 23.0 39.0 80-81 31.845235883139388 35.0 32.5 36.5 21.5 39.0 82-83 31.422487999604208 35.0 32.0 36.0 20.0 37.5 84-85 31.03231779648341 35.0 32.0 35.5 18.5 37.0 86-87 30.66222395062694 35.0 31.0 35.0 16.5 36.5 88-89 30.276870157558406 34.0 31.0 35.0 9.0 36.0 90-91 29.91163960728906 34.0 31.0 35.0 4.0 36.0 92-93 29.52115443692486 34.0 30.5 35.0 2.0 35.5 94-95 29.094098693187966 34.0 30.0 35.0 2.0 35.0 96-97 28.579871665949483 34.0 29.5 35.0 2.0 35.0 98-99 27.948447916211236 34.0 29.0 35.0 2.0 35.0 100-101 25.42497897341964 31.5 23.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27467353217649304 1101 2 0.3259126231443048 1101 3 0.24988323426478587 1101 4 0.20248938124698412 1101 5 0.30997009831853717 1101 6 0.28039390309635337 1101 7 0.2184920891495281 1101 8 0.36254038647810205 1101 9 0.37984739650019606 1101 10-11 0.4204634373917884 1101 12-13 0.39562735716345543 1101 14-15 0.4913743568964719 1101 16-17 0.5344278315863633 1101 18-19 0.5176759290737607 1101 20-21 0.5477209391810334 1101 22-23 0.49267570026021446 1101 24-25 0.49401222729463257 1101 26-27 0.5450477254901429 1101 28-29 0.5054597848160185 1101 30-31 0.5567234012158195 1101 32-33 0.5303482085265543 1101 34-35 0.5014499736556104 1101 36-37 0.4619748506390451 1101 38-39 0.535733477089984 1101 40-41 0.3974231747416326 1101 42-43 0.3452063985950957 1101 44-45 0.4502089019875726 1101 46-47 0.40100246324114863 1101 48-49 0.3745372018858433 1101 50-51 0.3457728926188963 1101 52-53 0.48706556604867046 1101 54-55 0.47402940688579775 1101 56-57 0.4556184204269087 1101 58-59 0.43689741823283157 1101 60-61 0.3672010752435426 1101 62-63 0.471542705066895 1101 64-65 0.4428470231824946 1101 66-67 0.33808683709755627 1101 68-69 0.3858627884575512 1101 70-71 0.4444127820521331 1101 72-73 0.26583033233264786 1101 74-75 0.2104024777855784 1101 76-77 0.2678849261423224 1101 78-79 0.20274817209862306 1101 80-81 0.14038074934828515 1101 82-83 0.18270104149852884 1101 84-85 0.2338028813112345 1101 86-87 0.19391375307546 1101 88-89 0.18329946566777622 1101 90-91 0.06993656813239468 1101 92-93 0.0928964348200303 1101 94-95 0.008234050097460255 1101 96-97 -0.08689176579754232 1101 98-99 -0.2021981779249451 1101 100-101 -0.1528387775877995 1102 1 0.1984423839795042 1102 2 0.263445669149597 1102 3 0.25835460095353824 1102 4 0.28539729851225104 1102 5 0.29047464986593496 1102 6 0.27484512620855384 1102 7 0.25348258808134005 1102 8 0.33030366665356325 1102 9 0.37708629205841504 1102 10-11 0.29642715621265125 1102 12-13 0.31375810673104354 1102 14-15 0.43957981394267875 1102 16-17 0.4561351483928675 1102 18-19 0.4540383428917423 1102 20-21 0.4658766714192808 1102 22-23 0.47573263888265416 1102 24-25 0.4252903669658039 1102 26-27 0.43688391351727063 1102 28-29 0.5034660364970165 1102 30-31 0.590179078568724 1102 32-33 0.5053007031856254 1102 34-35 0.4587381894883862 1102 36-37 0.4207113507035487 1102 38-39 0.3795317067643751 1102 40-41 0.37364984027122716 1102 42-43 0.4043303213882581 1102 44-45 0.45929915010867717 1102 46-47 0.4690721501322983 1102 48-49 0.6236957177512039 1102 50-51 0.5631148567269406 1102 52-53 0.5664739414206821 1102 54-55 0.5118813236087618 1102 56-57 0.5809143311409244 1102 58-59 0.5171520884069167 1102 60-61 0.4822339189821321 1102 62-63 0.4697142141076682 1102 64-65 0.5150919867416874 1102 66-67 0.48379249641046584 1102 68-69 0.38534279904101965 1102 70-71 0.3335333483114269 1102 72-73 0.19360701677692305 1102 74-75 0.15092387760233095 1102 76-77 0.17974005070581 1102 78-79 0.1849268359138705 1102 80-81 0.16146770872219562 1102 82-83 0.0728733399260939 1102 84-85 0.15380016984413558 1102 86-87 0.038709540242788165 1102 88-89 0.040589922834247005 1102 90-91 -0.03309273101896082 1102 92-93 -0.15958144157642806 1102 94-95 -0.15791147915249226 1102 96-97 -0.14684979272416143 1102 98-99 -0.37787101536593326 1102 100-101 -0.41343509261654177 1103 1 0.21224209344595124 1103 2 0.30450478448126717 1103 3 0.34400511911400145 1103 4 0.24268886606130025 1103 5 0.23327802377669826 1103 6 0.2822489741922567 1103 7 0.24396465387217603 1103 8 0.33496954249381616 1103 9 0.33582228079231413 1103 10-11 0.3548153081951071 1103 12-13 0.4141270885737569 1103 14-15 0.4923557943876986 1103 16-17 0.5084268005222796 1103 18-19 0.49593829277788615 1103 20-21 0.5053296584806048 1103 22-23 0.5062759831480008 1103 24-25 0.5004417658193105 1103 26-27 0.48617809037057924 1103 28-29 0.4633849344994374 1103 30-31 0.4689888425260733 1103 32-33 0.5134211583125747 1103 34-35 0.5143670656479316 1103 36-37 0.4218986475785229 1103 38-39 0.4859925365400457 1103 40-41 0.463078880516683 1103 42-43 0.30135240532158036 1103 44-45 0.17958791804763763 1103 46-47 0.26088376639964395 1103 48-49 0.25851137484549014 1103 50-51 0.3913198182111586 1103 52-53 0.4050962964340741 1103 54-55 0.4068162813080036 1103 56-57 0.4106211755955158 1103 58-59 0.3592211461605004 1103 60-61 0.37934925276797316 1103 62-63 0.37486854155632443 1103 64-65 0.3250110433322959 1103 66-67 0.37118679738546945 1103 68-69 0.2891563162520612 1103 70-71 0.19044652445215604 1103 72-73 0.14235353576626864 1103 74-75 0.13714134852254745 1103 76-77 0.1615253519070592 1103 78-79 0.225399597864552 1103 80-81 0.17050073312574554 1103 82-83 0.09478372491477316 1103 84-85 0.16735396189907803 1103 86-87 0.22556020092116214 1103 88-89 0.287616394374016 1103 90-91 0.07056991596585149 1103 92-93 -0.09756061577603603 1103 94-95 -0.10377830102412844 1103 96-97 -0.0923373773838172 1103 98-99 -0.17746032483068674 1103 100-101 -0.2822459244009998 1104 1 0.25349766737703305 1104 2 0.29175176590489116 1104 3 0.2834774875737551 1104 4 0.1645678605705072 1104 5 0.2360958142036722 1104 6 0.287079148870383 1104 7 0.18290536376079558 1104 8 0.24669838304827607 1104 9 0.33559943666782033 1104 10-11 0.30339544045366296 1104 12-13 0.35072963193785966 1104 14-15 0.39091307663957764 1104 16-17 0.38085758875701714 1104 18-19 0.4090256906598455 1104 20-21 0.34306972494923116 1104 22-23 0.40106244870444385 1104 24-25 0.4571607989699231 1104 26-27 0.361751885711449 1104 28-29 0.4372160209154501 1104 30-31 0.36375211871930446 1104 32-33 0.330164104786995 1104 34-35 0.32165271109275295 1104 36-37 0.38957210967125633 1104 38-39 0.49271236152257103 1104 40-41 0.4654362772448337 1104 42-43 0.4734994914932571 1104 44-45 0.33519872587455524 1104 46-47 0.37459082931408005 1104 48-49 0.39157307212588677 1104 50-51 0.4521025327467072 1104 52-53 0.42587170349223413 1104 54-55 0.4258117633413434 1104 56-57 0.2877735444172487 1104 58-59 0.3626379008713414 1104 60-61 0.3245024496196507 1104 62-63 0.31935999316521446 1104 64-65 0.30110846580629413 1104 66-67 0.30020019744383575 1104 68-69 0.3280414847878248 1104 70-71 0.20166904505674665 1104 72-73 0.20157916940147658 1104 74-75 0.21738559473249808 1104 76-77 0.28101109101428534 1104 78-79 0.21187567436003718 1104 80-81 0.20485831989355674 1104 82-83 0.2211091949801336 1104 84-85 0.2771872699692004 1104 86-87 0.22219152171942014 1104 88-89 0.27315415005088184 1104 90-91 0.23318730504717067 1104 92-93 0.15443480064836734 1104 94-95 0.06818213905342319 1104 96-97 8.863723565966097E-5 1104 98-99 -0.10698558766232225 1104 100-101 -0.16793141328933459 1105 1 0.21802129454773223 1105 2 0.3222137444707158 1105 3 0.32926611752245805 1105 4 0.26603779325918 1105 5 0.2907583497789332 1105 6 0.26761469637907 1105 7 0.30920239624233403 1105 8 0.3041192898389937 1105 9 0.3213205735361484 1105 10-11 0.38015841283047536 1105 12-13 0.4572724896335032 1105 14-15 0.5262015648051275 1105 16-17 0.43662229047789936 1105 18-19 0.49407047009860605 1105 20-21 0.4410974831787655 1105 22-23 0.43595326981358085 1105 24-25 0.4441567627914722 1105 26-27 0.4601486123673979 1105 28-29 0.47054454020099 1105 30-31 0.46059040325845046 1105 32-33 0.5104905832159261 1105 34-35 0.48050597950223306 1105 36-37 0.45667614137440893 1105 38-39 0.5215752000506129 1105 40-41 0.4499075531114656 1105 42-43 0.4461843465314601 1105 44-45 0.35359154787524005 1105 46-47 0.4007185037244625 1105 48-49 0.49326061410630473 1105 50-51 0.4221921991097446 1105 52-53 0.4435012806892047 1105 54-55 0.4445497603860744 1105 56-57 0.4863356717234737 1105 58-59 0.4417204104585579 1105 60-61 0.5600312362900368 1105 62-63 0.47309651179567425 1105 64-65 0.45524888565250166 1105 66-67 0.5409715407918227 1105 68-69 0.5490249908438543 1105 70-71 0.5334450436996931 1105 72-73 0.5517684122911746 1105 74-75 0.49460517530842196 1105 76-77 0.5619062361675944 1105 78-79 0.4365809149038 1105 80-81 0.43022972628667944 1105 82-83 0.4768619658098956 1105 84-85 0.5844417696544646 1105 86-87 0.5188092069154813 1105 88-89 0.3888476396928944 1105 90-91 0.3616426464807425 1105 92-93 0.3102809177540209 1105 94-95 0.18519960583893535 1105 96-97 0.25100221517604027 1105 98-99 0.24171688639995992 1105 100-101 0.20589995055125243 1106 1 0.177437474562133 1106 2 0.26595227568633817 1106 3 0.24112431567443338 1106 4 0.24208227855842068 1106 5 0.2553567065320195 1106 6 0.17097515716830003 1106 7 0.24353798555723216 1106 8 0.23401863808985723 1106 9 0.23957309389810888 1106 10-11 0.275827487483987 1106 12-13 0.3001276631734342 1106 14-15 0.31547052820425137 1106 16-17 0.37365778162220664 1106 18-19 0.3857870021972687 1106 20-21 0.4185687481485587 1106 22-23 0.42640293641044735 1106 24-25 0.396845025742401 1106 26-27 0.42001005313242956 1106 28-29 0.35184542171626987 1106 30-31 0.3624885925781598 1106 32-33 0.4383583416146877 1106 34-35 0.4405569904293145 1106 36-37 0.47682267059911965 1106 38-39 0.4577576254656961 1106 40-41 0.4507277924337387 1106 42-43 0.38124646559667497 1106 44-45 0.46685242494259427 1106 46-47 0.5344191498996551 1106 48-49 0.5140246725762125 1106 50-51 0.44652956628787877 1106 52-53 0.36821241999840026 1106 54-55 0.32293442246720616 1106 56-57 0.3823178597970909 1106 58-59 0.388572727446423 1106 60-61 0.43929108217800916 1106 62-63 0.4904397500374813 1106 64-65 0.3599242731701082 1106 66-67 0.336643149905818 1106 68-69 0.4239806494757872 1106 70-71 0.35946765875601727 1106 72-73 0.40737814129000327 1106 74-75 0.28733017016702433 1106 76-77 0.3945552963456933 1106 78-79 0.4432906418357945 1106 80-81 0.598522624999628 1106 82-83 0.61967133201615 1106 84-85 0.6059245019824253 1106 86-87 0.5043765767562043 1106 88-89 0.6406453998470809 1106 90-91 0.5116987927297707 1106 92-93 0.6104151880459092 1106 94-95 0.6031307827984271 1106 96-97 0.7422001087494614 1106 98-99 0.8369592925811986 1106 100-101 0.6673552957424285 1107 1 0.0681369642244718 1107 2 0.13152466124687834 1107 3 0.09611765970697661 1107 4 0.15032571849507548 1107 5 0.23253075738490026 1107 6 0.19747124546769612 1107 7 0.1835015125412056 1107 8 0.18058665741912705 1107 9 0.25369005241868336 1107 10-11 0.20360618510749617 1107 12-13 0.287481143756402 1107 14-15 0.3208800820391886 1107 16-17 0.3343400407161923 1107 18-19 0.2676035008584208 1107 20-21 0.17290106294532848 1107 22-23 0.24152404928059212 1107 24-25 0.2778806524097419 1107 26-27 0.24675529728078516 1107 28-29 0.13680769308816565 1107 30-31 0.23242005002433075 1107 32-33 0.26275308538962605 1107 34-35 0.21772336532816183 1107 36-37 0.2101592120643616 1107 38-39 0.3190842659471187 1107 40-41 0.2220289953291399 1107 42-43 0.185509683213418 1107 44-45 0.1537895915041787 1107 46-47 0.31562237880286403 1107 48-49 0.34161218728807796 1107 50-51 0.2993906807294664 1107 52-53 0.27830348110072123 1107 54-55 0.41760027785712595 1107 56-57 0.33758345097831466 1107 58-59 0.27435783933999147 1107 60-61 0.25422790871385814 1107 62-63 0.22909145919695106 1107 64-65 0.240495445712412 1107 66-67 0.1739049041975278 1107 68-69 0.3018841787743227 1107 70-71 0.3593766257475153 1107 72-73 0.3367784408425294 1107 74-75 0.3872741728589517 1107 76-77 0.3132109368261169 1107 78-79 0.31092343652015586 1107 80-81 0.2754573528372184 1107 82-83 0.2961605010818893 1107 84-85 0.27063593006421627 1107 86-87 0.14712961068383024 1107 88-89 0.18347835613428742 1107 90-91 0.1284196487318674 1107 92-93 0.23786244214327823 1107 94-95 0.26297332695940767 1107 96-97 0.3644877986126396 1107 98-99 0.4246833486495589 1107 100-101 0.45599708597428545 1108 1 -0.029163621819591867 1108 2 0.05273743270713993 1108 3 0.09162094799992104 1108 4 0.0965419027668375 1108 5 0.11379544719413559 1108 6 0.13694572047261033 1108 7 0.21100323421192257 1108 8 0.18044194548876646 1108 9 0.22561785695377523 1108 10-11 0.20119370900263078 1108 12-13 0.23893059641994085 1108 14-15 0.3247717775021428 1108 16-17 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-0.8913785023253986 2316 96-97 -1.0302938590559272 2316 98-99 -1.0331970025101 2316 100-101 -0.903671683120514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8612.0 3 1685.0 4 1717.0 5 2029.0 6 2396.0 7 2812.0 8 3410.0 9 4403.0 10 5441.0 11 7204.0 12 10428.0 13 17615.0 14 15008.0 15 14630.0 16 17848.0 17 23639.0 18 22645.0 19 14477.0 20 14546.0 21 15164.0 22 16562.0 23 18641.0 24 21250.0 25 24893.0 26 28547.0 27 33997.0 28 41025.0 29 48432.0 30 59358.0 31 73205.0 32 92761.0 33 120847.0 34 168483.0 35 249758.0 36 415071.0 37 759318.0 38 907881.0 39 190724.0 40 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8833412951206 19.277377603757 13.167631943171601 23.671649157950796 2 15.16352556274912 20.951172729995623 39.97894730778979 23.906354399465474 3 19.6534620105274 24.77461236436857 29.834912520280998 25.73701310482303 4 12.339423647391552 16.227355724645612 38.249773917250835 33.183446710712 5 13.095107100410704 38.21521015369849 34.798315014221096 13.891367731669702 6 31.54157403264748 36.294541344283004 17.999905566563193 14.16397905650632 7 28.0306969732045 32.97246854778197 21.418288153972046 17.57854632504148 8 25.69361998899457 35.28829937304752 20.698934097282592 18.319146540675316 9 26.171216173807395 16.850057726580427 21.249773268418522 35.72895283119366 10-11 24.535217143389364 26.929672815498623 28.109438949614567 20.425671091497446 12-13 25.314069067698647 24.394888173762475 28.34594081943429 21.94510193910459 14-15 22.15537725004337 25.739279655008346 26.990255900481625 25.115087194466657 16-17 21.615157245976473 28.969021798430234 27.5637561691973 21.852064786395996 18-19 21.310872865231435 28.08622921667181 29.55617890823317 21.046719009863587 20-21 22.3012835991054 27.125496221367246 29.298539416293494 21.274680763233853 22-23 21.81809332624298 27.27664133925271 29.096056546558362 21.80920878794594 24-25 21.72025165000579 27.20539396498537 29.461251579149295 21.613102805859544 26-27 21.653524823279266 27.37572806753264 29.44666291459741 21.524084194590685 28-29 21.31442309838588 27.87910775219307 29.34819596963437 21.45827317978668 30-31 21.61618570221501 27.521470753064598 29.27177414570565 21.590569399014743 32-33 21.30669305583812 27.618525578476184 29.28404686180082 21.790734503884874 34-35 22.00938370648902 27.473620591971265 29.105782876525122 21.411212825014598 36-37 21.695625312769856 27.242568906216892 29.257450029820646 21.80435575119261 38-39 21.48087389525361 27.714231708659966 29.359945802794655 21.444948593291766 40-41 21.742915102884208 27.489110410590506 29.267159419774764 21.50081506675052 42-43 21.415971896136412 27.649547468772003 28.934596544596037 21.99988409049555 44-45 21.66846109784499 27.729287906401083 28.795221942157017 21.807029053596906 46-47 21.713636569945756 27.708937604128753 28.549527487099812 22.027898338825675 48-49 21.784713537303375 27.78973889176492 28.59040990279043 21.835137668141275 50-51 21.434019843214923 27.979557490438534 28.6667371095641 21.919685556782444 52-53 21.835734295018987 28.08142931051007 28.3093613003919 21.773475094079046 54-55 21.390613234203755 27.67670825811655 28.695628923136514 22.237049584543186 56-57 21.650704428870746 28.070645184596486 28.396806589119283 21.88184379741348 58-59 21.93221476616722 27.665517992120943 28.479905274440178 21.922361967271662 60-61 21.751589634106892 27.683792779179488 28.817042471881038 21.74757511483258 62-63 21.598871980902175 27.69489493898688 28.75587395539804 21.950359124712904 64-65 21.397636272825377 27.733497748091246 28.645638250428444 22.223227728654933 66-67 21.423002250864805 27.891394074539495 28.325649492828937 22.359954181766767 68-69 21.480888116443282 27.67968698601862 28.486123090203346 22.353301807334745 70-71 21.413608333992382 27.570703332778624 28.56725637858577 22.448431954643226 72-73 21.554679439184646 27.32077390911248 28.684559506721204 22.43998714498168 74-75 21.27488018043417 27.679888385273216 28.531528991636122 22.513702442656488 76-77 21.30000734286132 27.391358897668844 28.62892011949784 22.679713639971993 78-79 21.337844251079943 27.569884927376552 28.544580164409282 22.54769065713422 80-81 21.267872234406486 27.487776877285512 28.535456593896356 22.708894294411643 82-83 21.4465475345975 27.383392968745028 28.59392244910466 22.57613704755281 84-85 21.29879139843382 27.179630973269695 28.572803718596163 22.94877390970032 86-87 21.177962250536826 27.19239668504071 28.765103080803 22.864537983619467 88-89 21.242998633336697 27.129206204830652 28.558449214450494 23.06934594738216 90-91 21.187367220575126 27.045999549486115 28.80115306107991 22.96548016885885 92-93 21.410203678899062 27.025487150165205 28.73473600968048 22.829573161255254 94-95 21.155234375192016 26.790370969777676 28.75636375623147 23.298030898798835 96-97 21.12208625420556 26.556712185695545 28.971375391965665 23.349826168133227 98-99 21.03675042875187 26.586562627987654 28.685874615503938 23.690812327756536 100-101 21.099488382137523 26.207124142135925 29.20350144708324 23.489886028643316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3407.0 1 1935.5 2 547.5 3 616.0 4 542.0 5 556.5 6 926.0 7 1687.5 8 2565.5 9 3093.5 10 3051.5 11 3016.5 12 3305.0 13 3694.5 14 4019.5 15 4289.5 16 4513.5 17 4759.0 18 5013.5 19 5226.0 20 5588.5 21 6382.0 22 7575.5 23 9370.0 24 11759.5 25 14723.0 26 19510.5 27 26029.0 28 31169.5 29 36407.0 30 43723.0 31 50750.0 32 58505.5 33 67461.0 34 77094.5 35 85275.5 36 95026.5 37 104365.0 38 108145.5 39 110629.0 40 113307.5 41 118425.5 42 123409.0 43 127208.5 44 131813.0 45 135214.5 46 137039.0 47 141399.5 48 157162.0 49 158010.5 50 140315.5 51 127620.5 52 115659.5 53 107928.5 54 100961.0 55 90038.5 56 79540.0 57 68848.5 58 58374.0 59 49875.5 60 41754.5 61 32401.5 62 23959.0 63 18120.0 64 13578.0 65 10124.5 66 7470.5 67 5652.0 68 4223.5 69 2948.0 70 2202.0 71 1674.5 72 1339.0 73 1141.0 74 897.0 75 705.0 76 501.5 77 310.0 78 221.0 79 174.0 80 124.0 81 78.0 82 54.5 83 42.5 84 34.5 85 28.0 86 15.5 87 11.0 88 11.0 89 10.0 90 10.0 91 8.5 92 5.0 93 3.5 94 2.5 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3476720612837087 2 0.12403093639905284 3 0.11764529913546526 4 0.1257567843081306 5 0.1423824524989127 6 0.0922178066083867 7 0.10084704615377536 8 0.10703133449463721 9 0.09440388062655182 10-11 0.09309511262883455 12-13 0.09638860572199122 14-15 0.09850276941061142 16-17 0.08758678138569478 18-19 0.10286053538103271 20-21 0.11314371250595419 22-23 0.12160036726043505 24-25 0.13651456960804845 26-27 0.13490377822624255 28-29 0.13043095572854946 30-31 0.11948620357181483 32-33 0.11262595813323086 34-35 0.3400783304837667 36-37 0.5044941079552384 38-39 0.4773407675190821 40-41 0.45425755173516746 42-43 0.48773900117127544 44-45 0.49585048634394074 46-47 0.5031709578916121 48-49 0.5146047002892521 50-51 0.5029552269029774 52-53 0.5069822053574922 54-55 0.49206800300987874 56-57 0.46891287689641925 58-59 0.4489074232170266 60-61 0.40614954126962577 62-63 0.47982886492133586 64-65 0.5125624469301768 66-67 0.5207314603664781 68-69 0.5338479044754688 70-71 0.5258514758300753 72-73 0.5171934721528687 74-75 0.5242838306459964 76-77 0.5007835349507213 78-79 0.47892279476907 80-81 0.48082122746905553 82-83 0.4946855390053133 84-85 0.4942253128962259 86-87 0.49830981961437654 88-89 0.510937273482462 90-91 0.5257795655005304 92-93 0.5190343765892182 94-95 0.5234352887573666 96-97 0.5229750626482791 98-99 0.5167907743074173 100-101 0.504091410109787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3476552.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.11351514512023 #Duplication Level Percentage of deduplicated Percentage of total 1 68.82540998926434 32.42606995898315 2 15.525617875867272 14.629328658640445 3 6.284810772869973 8.882985825954728 4 3.268540055696762 6.159696456660061 5 1.7812111685557779 4.195955968320497 6 1.1218297874485523 3.1712006808722633 7 0.7042169986921434 2.322469676333339 8 0.49239396570343164 1.8558728448427548 9 0.33397315362852015 1.4161184308386765 >10 1.4884758033612573 12.079103900435985 >50 0.1014549061163883 3.3135584349722733 >100 0.06552496545029027 5.808068136429781 >500 0.0042177425918298065 1.3482799287685108 >1k 0.0021394346412908025 1.6801300991672456 >5k 1.833801121106402E-4 0.7111609987801846 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9510 0.27354689358882023 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7762 0.22326719117102234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7422 0.2134873863529152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4660 0.13404085427170367 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3617 0.10403986478556915 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.752826363592433E-5 0.0 12-13 0.0 0.0 0.0 7.191032954490541E-5 0.0 14-15 0.0 0.0 0.0 8.62923954538865E-5 0.0 16-17 0.0 0.0 0.0 1.1505652727184866E-4 0.0 18-19 0.0 0.0 0.0 1.72584790907773E-4 0.0 20-21 0.0 0.0 0.0 2.8764131817962164E-4 0.0 22-23 0.0 0.0 0.0 4.602261090873946E-4 0.0 24-25 0.0 0.0 0.0 8.773060204478461E-4 0.0 26-27 0.0 0.0 0.0 0.0013231500636262596 0.0 28-29 1.4382065908981083E-5 0.0 0.0 0.004257091509058401 0.0 30-31 2.8764131817962166E-5 0.0 0.0 0.013835547404439801 0.0 32-33 2.8764131817962166E-5 0.0 0.0 0.03293493093156668 0.0 34-35 2.8764131817962166E-5 0.0 0.0 0.06434536287678136 0.0 36-37 2.8764131817962166E-5 0.0 0.0 0.12206059336952245 0.0 38-39 2.8764131817962166E-5 0.0 0.0 0.2076914137915958 0.0 40-41 2.8764131817962166E-5 0.0 0.0 0.30395058092040617 0.0 42-43 2.8764131817962166E-5 0.0 0.0 0.40530099938099584 0.0 44-45 2.8764131817962166E-5 0.0 0.0 0.5301229494050427 0.0 46-47 2.8764131817962166E-5 0.0 0.0 0.6794663218039023 0.0 48-49 2.8764131817962166E-5 0.0 0.0 0.8416528790594819 0.0 50-51 2.8764131817962166E-5 0.0 0.0 1.0162655412604213 0.0 52-53 2.8764131817962166E-5 0.0 0.0 1.195164059102237 0.0 54-55 2.8764131817962166E-5 0.0 0.0 1.3932770169984514 0.0 56-57 2.8764131817962166E-5 0.0 0.0 1.6333568432170726 0.0 58-59 2.8764131817962166E-5 0.0 0.0 1.8993387701377686 0.0 60-61 2.8764131817962166E-5 0.0 0.0 2.187195819306025 0.0 62-63 2.8764131817962166E-5 0.0 0.0 2.487665940276458 0.0 64-65 2.8764131817962166E-5 0.0 0.0 2.797714517142272 0.0 66-67 2.8764131817962166E-5 0.0 0.0 3.1569641414827103 0.0 68-69 2.8764131817962166E-5 0.0 0.0 3.560870080470535 0.0 70-71 2.8764131817962166E-5 0.0 0.0 4.010568517312556 0.0 72-73 2.8764131817962166E-5 0.0 0.0 4.472937554220389 0.0 74-75 2.8764131817962166E-5 0.0 0.0 4.947516965084946 0.0 76-77 2.8764131817962166E-5 0.0 0.0 5.464336503524182 0.0 78-79 2.8764131817962166E-5 0.0 0.0 6.051743221444696 0.0 80-81 2.8764131817962166E-5 2.8764131817962166E-5 0.0 6.681850293048975 0.0 82-83 2.8764131817962166E-5 2.8764131817962166E-5 0.0 7.3232760505236225 0.0 84-85 2.8764131817962166E-5 2.8764131817962166E-5 0.0 7.974021386707289 0.0 86-87 2.8764131817962166E-5 2.8764131817962166E-5 0.0 8.670803715865604 0.0 88-89 2.8764131817962166E-5 2.8764131817962166E-5 0.0 9.417894511573536 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14135 0.0 26.432991 1 TACATGG 13875 0.0 25.982431 2 ACATGGG 13880 0.0 24.846775 3 CATGGGG 5395 0.0 21.776514 4 AGTACAT 10315 0.0 20.850414 2 GTATCAA 20550 0.0 20.51486 1 GAGTACT 9300 0.0 19.959797 12-13 GAGTACA 12490 0.0 19.689503 1 CATGGGA 9010 0.0 19.506403 4 GTGTAGC 2475 0.0 18.414648 1 ATCAACG 22770 0.0 18.329094 3 CAACGCA 22730 0.0 18.319664 5 TCAACGC 22825 0.0 18.284927 4 GTACTTT 10330 0.0 18.016361 14-15 AACGCAG 23255 0.0 17.899124 6 TATCAAC 23470 0.0 17.883343 2 AGTACTT 9870 0.0 17.79958 12-13 GGTATCA 16270 0.0 17.6245 1 ATGGGGA 3460 0.0 17.114412 5 AGAGTAC 17620 0.0 16.64877 10-11 >>END_MODULE