FastQCFastQC Report
Fri 27 May 2016
SRR939191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476552
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96310.2770273535387936No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81690.2349741928209329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75660.21762942133470173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.14246874489436662No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG150400.026.0898691
TACATGG149200.025.5358242
ACATGGG147550.024.8566973
GTATCAA203500.022.4412331
TAGCGCA1353.8260387E-421.0881929
CATGGGG55000.020.8764084
AGTACAT109350.020.2628822
GAGTACT103200.020.14002612-13
CATGGGA97700.020.0080974
GGTATCA158050.019.554321
ATCAACG231850.019.502443
GAGTACA127450.019.429251
CAACGCA232650.019.4149845
TCAACGC233050.019.3816594
AACGCAG238200.019.0422926
AGTACTT106500.019.0258412-13
TATCAAC239050.018.9745852
GTACTTT113700.018.21752414-15
AGAGTAC186400.017.99861510-11
CTGGACG12450.017.530394
Produced by FastQC (version 0.11.5)