##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3476552 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.542488362032266 34.0 31.0 34.0 31.0 34.0 2 32.74376853848295 34.0 31.0 34.0 31.0 34.0 3 32.84444558861769 34.0 31.0 34.0 31.0 34.0 4 36.28182779949789 37.0 37.0 37.0 35.0 37.0 5 36.247889575648514 37.0 37.0 37.0 35.0 37.0 6 36.28401933870111 37.0 37.0 37.0 35.0 37.0 7 36.237291718921504 37.0 37.0 37.0 35.0 37.0 8 36.22511614956428 37.0 37.0 37.0 35.0 37.0 9 37.9811620824311 39.0 38.0 39.0 35.0 39.0 10-11 37.930844699000616 39.0 38.0 39.0 35.0 39.0 12-13 37.87460808870398 39.0 38.0 39.0 35.0 39.0 14-15 39.27640187749242 41.0 39.0 41.0 36.0 41.0 16-17 39.229952550688154 41.0 39.0 41.0 36.0 41.0 18-19 39.25500150724051 40.5 39.0 41.0 36.0 41.0 20-21 39.31485161159678 41.0 39.0 41.0 36.0 41.0 22-23 39.27596293684087 41.0 39.0 41.0 36.0 41.0 24-25 39.23685076478074 41.0 39.0 41.0 36.0 41.0 26-27 39.121247719004344 41.0 39.0 41.0 35.5 41.0 28-29 38.99001812715588 40.0 39.0 41.0 35.0 41.0 30-31 38.83389533652884 40.0 38.0 41.0 35.0 41.0 32-33 38.71579096184956 40.0 38.0 41.0 35.0 41.0 34-35 38.672040285892464 40.0 38.0 41.0 35.0 41.0 36-37 38.58394481083556 40.0 38.0 41.0 35.0 41.0 38-39 38.471801802475554 40.0 38.0 41.0 34.5 41.0 40-41 38.339773862148476 40.0 38.0 41.0 34.0 41.0 42-43 38.20504741479489 40.0 38.0 41.0 34.0 41.0 44-45 38.01927671439979 40.0 38.0 41.0 33.0 41.0 46-47 37.77987658461602 40.0 38.0 41.0 33.0 41.0 48-49 37.52380073705211 40.0 37.5 41.0 33.0 41.0 50-51 37.31972885778783 40.0 37.0 41.0 32.5 41.0 52-53 37.13495656040813 40.0 37.0 41.0 32.0 41.0 54-55 36.93104964343982 40.0 37.0 41.0 31.0 41.0 56-57 36.696908603697 39.5 36.0 41.0 31.0 41.0 58-59 36.43588446253645 39.0 36.0 41.0 31.0 41.0 60-61 36.12147279833582 39.0 35.0 40.0 30.0 41.0 62-63 35.776963353345494 38.0 35.0 40.0 29.0 41.0 64-65 35.405183354081856 38.0 35.0 40.0 29.0 41.0 66-67 35.071949304943516 37.5 34.5 40.0 28.5 41.0 68-69 34.62927406234683 37.0 34.0 39.0 28.0 41.0 70-71 34.23354849862737 36.0 34.0 39.0 28.0 40.0 72-73 33.79228255466911 36.0 34.0 39.0 26.5 40.0 74-75 33.314047654112464 35.0 33.0 37.5 26.0 39.5 76-77 31.28381108638674 34.0 30.5 36.0 23.0 38.5 78-79 32.25330701223511 35.0 32.0 36.0 25.0 39.0 80-81 32.27212263760185 35.0 33.0 36.0 26.0 38.5 82-83 31.981102828319553 35.0 32.5 36.0 25.0 37.0 84-85 31.607164656245615 35.0 32.0 35.5 24.5 37.0 86-87 31.09197848903166 34.5 31.5 35.0 22.0 36.5 88-89 30.57643521512119 34.0 31.0 35.0 19.0 36.0 90-91 30.15568413761681 34.0 31.0 35.0 17.0 36.0 92-93 29.921311978074826 34.0 31.0 35.0 12.5 35.0 94-95 29.651192906074755 34.0 30.5 35.0 4.5 35.0 96-97 29.186962973658957 34.0 30.0 35.0 2.0 35.0 98-99 28.524523723505357 34.0 29.5 35.0 2.0 35.0 100-101 25.0721388605722 31.5 23.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20862821866705872 1101 2 0.20442934567510207 1101 3 0.21288439560828465 1101 4 0.1540319961205867 1101 5 0.20853816748959986 1101 6 0.20981312961598775 1101 7 0.18118833263863365 1101 8 0.18706804752220307 1101 9 0.20379554007569567 1101 10-11 0.27546561923637825 1101 12-13 0.23628904400121797 1101 14-15 0.3395531425974099 1101 16-17 0.29785041005698076 1101 18-19 0.30800383480517013 1101 20-21 0.3953445619483489 1101 22-23 0.2963551087045957 1101 24-25 0.2738703589416005 1101 26-27 0.26404123855799355 1101 28-29 0.23837697033776095 1101 30-31 0.23692500434711405 1101 32-33 0.2424906299263796 1101 34-35 0.4098339452316182 1101 36-37 0.47380455890790074 1101 38-39 0.44101693582828716 1101 40-41 0.5043429346470063 1101 42-43 0.44945249836475654 1101 44-45 0.5172610311801549 1101 46-47 0.47063185997432555 1101 48-49 0.5211716506403903 1101 50-51 0.4827653985254301 1101 52-53 0.49129512054552293 1101 54-55 0.5545894360027788 1101 56-57 0.48017221491159745 1101 58-59 0.46596727468650556 1101 60-61 0.5296580009905014 1101 62-63 0.4738765207597879 1101 64-65 0.4548129069111866 1101 66-67 0.5627696133327404 1101 68-69 0.46047539581882546 1101 70-71 0.5247320155368485 1101 72-73 0.6554333641682462 1101 74-75 0.68810195985278 1101 76-77 0.6642844787664792 1101 78-79 0.7060157431461604 1101 80-81 0.6503684390152955 1101 82-83 0.712913129276437 1101 84-85 0.5661812339072867 1101 86-87 0.7287586290124857 1101 88-89 0.7949475862505153 1101 90-91 0.8450394145122644 1101 92-93 0.7333000420595539 1101 94-95 0.6863099071036203 1101 96-97 0.6032083013472018 1101 98-99 0.7409781145233936 1101 100-101 0.5781080214556198 1102 1 0.11054494164040563 1102 2 0.11739026774884564 1102 3 0.1534033354246489 1102 4 0.08256548601927705 1102 5 0.10878930619644933 1102 6 0.13175163179280958 1102 7 0.1156655114883165 1102 8 0.08285816684862368 1102 9 0.24367553578983348 1102 10-11 0.21761532064699196 1102 12-13 0.1854401251987241 1102 14-15 0.28649503410811406 1102 16-17 0.3479233354019797 1102 18-19 0.16634564147319253 1102 20-21 0.22083962336089513 1102 22-23 0.23760680063819706 1102 24-25 0.2054233626567452 1102 26-27 0.29542045691200514 1102 28-29 0.1748558241743794 1102 30-31 0.28459193997382215 1102 32-33 0.2669992858477599 1102 34-35 0.2242572008726711 1102 36-37 0.29609697314135275 1102 38-39 0.29407498965090895 1102 40-41 0.37298213896260535 1102 42-43 0.33276008369396237 1102 44-45 0.39929799897272744 1102 46-47 0.4687787690111591 1102 48-49 0.43298949253124874 1102 50-51 0.44711048082064053 1102 52-53 0.48268107383393755 1102 54-55 0.4306004961645442 1102 56-57 0.41830839834671707 1102 58-59 0.42642796169354824 1102 60-61 0.46906917529628345 1102 62-63 0.5092589250876429 1102 64-65 0.4191307652020484 1102 66-67 0.4198076744588306 1102 68-69 0.35158347839611537 1102 70-71 0.3861184826920194 1102 72-73 0.35641184299147 1102 74-75 0.4515173259992409 1102 76-77 0.45663426020911047 1102 78-79 0.43559752121257844 1102 80-81 0.34014112404558716 1102 82-83 0.3644809695272855 1102 84-85 0.34498155184866874 1102 86-87 0.4228139013946617 1102 88-89 0.4923006804936776 1102 90-91 0.5851799516014182 1102 92-93 0.48459542360015107 1102 94-95 0.45738221580852567 1102 96-97 0.6413684287861585 1102 98-99 0.5444337044096201 1102 100-101 0.23738162160606535 1103 1 0.1313809974042357 1103 2 0.13319738645527224 1103 3 0.17108549509968896 1103 4 0.12118731240538949 1103 5 0.15330004050203172 1103 6 0.14040874933625958 1103 7 0.12010238599008716 1103 8 0.15020961729615578 1103 9 0.21283288275751744 1103 10-11 0.21237361110865294 1103 12-13 0.24904545841364722 1103 14-15 0.2524739038184052 1103 16-17 0.25516975818116094 1103 18-19 0.3249619489904845 1103 20-21 0.2788182477881236 1103 22-23 0.22187252677120028 1103 24-25 0.23899547693327605 1103 26-27 0.23439625212602522 1103 28-29 0.28959691310429037 1103 30-31 0.29045526233285557 1103 32-33 0.37356467809978255 1103 34-35 0.3196164876910359 1103 36-37 0.35876319999012196 1103 38-39 0.35964475470780144 1103 40-41 0.419715909576432 1103 42-43 0.35098836821385504 1103 44-45 0.4005677617036696 1103 46-47 0.378000883393419 1103 48-49 0.43307893626732863 1103 50-51 0.3868298431692665 1103 52-53 0.43615267491910714 1103 54-55 0.4118696456878297 1103 56-57 0.43491390283688247 1103 58-59 0.4173207708823554 1103 60-61 0.4263321969721261 1103 62-63 0.4595097587328638 1103 64-65 0.4200864176021142 1103 66-67 0.4008375144116414 1103 68-69 0.38352393170458043 1103 70-71 0.29082004087378976 1103 72-73 0.35442499735275135 1103 74-75 0.29548415569455955 1103 76-77 0.43296872691933785 1103 78-79 0.40956928828671124 1103 80-81 0.2741277878365267 1103 82-83 0.3315349085406254 1103 84-85 0.32009721549793113 1103 86-87 0.3134594716037675 1103 88-89 0.3644763306523693 1103 90-91 0.4327869616220781 1103 92-93 0.40877684425425187 1103 94-95 0.35760270624777135 1103 96-97 0.2879087218148335 1103 98-99 0.357839692276567 1103 100-101 0.07339409086638682 1104 1 0.15532033855483718 1104 2 0.14813020517327402 1104 3 0.11686310797532684 1104 4 0.07676893557032116 1104 5 0.11397879881909745 1104 6 0.11149331019759501 1104 7 0.13304996897972643 1104 8 0.09677001257864504 1104 9 0.12468389166449612 1104 10-11 0.15756074361380712 1104 12-13 0.1755397017956284 1104 14-15 0.19467130525229948 1104 16-17 0.19634004050318055 1104 18-19 0.15949453061138996 1104 20-21 0.18429783775494002 1104 22-23 0.2567906174155965 1104 24-25 0.19398888210462673 1104 26-27 0.1862975536714515 1104 28-29 0.14269173158248094 1104 30-31 0.21207408349874868 1104 32-33 0.12291311355303236 1104 34-35 0.15055867717414628 1104 36-37 0.16700639645719662 1104 38-39 0.24433592894928324 1104 40-41 0.3340289501431144 1104 42-43 0.2961755007190092 1104 44-45 0.19756242023180448 1104 46-47 0.22038221606916153 1104 48-49 0.07397524474694706 1104 50-51 0.06087336677604327 1104 52-53 -0.023547605389389048 1104 54-55 -0.026048469842713473 1104 56-57 0.03538153614178441 1104 58-59 0.12276608583916016 1104 60-61 0.10842073282966425 1104 62-63 0.04446879839912299 1104 64-65 0.043492855874298186 1104 66-67 0.13281485444462504 1104 68-69 0.01675587284658775 1104 70-71 0.09248085175492093 1104 72-73 0.1068050178498865 1104 74-75 0.07455965799879749 1104 76-77 0.07325532482483155 1104 78-79 0.13842567938832673 1104 80-81 0.1509396689350453 1104 82-83 0.1540712366576642 1104 84-85 0.11440812943032341 1104 86-87 0.17910708830282474 1104 88-89 0.21397453545630185 1104 90-91 0.3378508398338056 1104 92-93 0.452705982085039 1104 94-95 0.47338832793763075 1104 96-97 0.3494023882907058 1104 98-99 0.3988362240527792 1104 100-101 0.1203446330450113 1105 1 0.11139795012527998 1105 2 0.17703686847336542 1105 3 0.16534786526035106 1105 4 0.1113563055543878 1105 5 0.09984924282608176 1105 6 0.06060654500515028 1105 7 0.1269590585208178 1105 8 0.12247800494678529 1105 9 0.0839263650896811 1105 10-11 0.15801267130772345 1105 12-13 0.21251774485197927 1105 14-15 0.22376636132944583 1105 16-17 0.18225142671638395 1105 18-19 0.19296742247373544 1105 20-21 0.19758964194605255 1105 22-23 0.3139781340880674 1105 24-25 0.20908629621832375 1105 26-27 0.2392164300609707 1105 28-29 0.24192332747450251 1105 30-31 0.21596388623157736 1105 32-33 0.221606646775534 1105 34-35 0.35141845373328096 1105 36-37 0.24735142102154128 1105 38-39 0.2933262589669141 1105 40-41 0.2345356270449983 1105 42-43 0.26472680521196423 1105 44-45 0.34027053513342054 1105 46-47 0.22918625611708165 1105 48-49 0.25190958439215194 1105 50-51 0.32112059516457236 1105 52-53 0.3493339774382562 1105 54-55 0.27737089814642246 1105 56-57 0.26464606754699815 1105 58-59 0.36170841673855847 1105 60-61 0.3855155325050248 1105 62-63 0.3951358632181581 1105 64-65 0.34899309439440884 1105 66-67 0.2483193650250044 1105 68-69 0.2613342062310551 1105 70-71 0.35266524997972937 1105 72-73 0.32213302745869754 1105 74-75 0.3329258900396752 1105 76-77 0.3526559817371542 1105 78-79 0.4790414814383297 1105 80-81 0.38649918668861716 1105 82-83 0.3350996442950773 1105 84-85 0.3869817467416681 1105 86-87 0.41053930921328785 1105 88-89 0.5808861290188183 1105 90-91 0.5156062649692714 1105 92-93 0.4794085573791236 1105 94-95 0.29380354835424427 1105 96-97 0.4174611355686366 1105 98-99 0.4677939410137384 1105 100-101 0.4458750886721603 1106 1 0.07196445722069456 1106 2 0.07366155630685967 1106 3 0.04950244640054535 1106 4 -0.005383375707744165 1106 5 0.022204502921120195 1106 6 0.07517810287815507 1106 7 0.07561687465197764 1106 8 0.06098918804322295 1106 9 0.08069759337059423 1106 10-11 0.13301143447230146 1106 12-13 0.13538101931319346 1106 14-15 0.11172380222016187 1106 16-17 0.09482268737479416 1106 18-19 0.06733627401056452 1106 20-21 0.12300168583318083 1106 22-23 0.12951173013864548 1106 24-25 0.1614044905302663 1106 26-27 0.10914843263733331 1106 28-29 0.16565916711179796 1106 30-31 0.23939964948903025 1106 32-33 0.21930262580254833 1106 34-35 0.12178618711476474 1106 36-37 0.24755478575630718 1106 38-39 0.3072744424367926 1106 40-41 0.26206206562955714 1106 42-43 0.2600101836122448 1106 44-45 0.3104920273395777 1106 46-47 0.3412240923387557 1106 48-49 0.3719450930251682 1106 50-51 0.2802475567644933 1106 52-53 0.320232946829357 1106 54-55 0.297665941143876 1106 56-57 0.36038587328761196 1106 58-59 0.41370801721032535 1106 60-61 0.25726509863063995 1106 62-63 0.3471309839608807 1106 64-65 0.5030296061500437 1106 66-67 0.549256572971693 1106 68-69 0.359392762742651 1106 70-71 0.42208304699862964 1106 72-73 0.217030073652559 1106 74-75 0.28603879202404414 1106 76-77 0.4263086658196471 1106 78-79 0.5649694180293139 1106 80-81 0.4144836518487196 1106 82-83 0.4662422155529313 1106 84-85 0.5070641569219276 1106 86-87 0.5134465878938634 1106 88-89 0.6991041208638435 1106 90-91 0.7720538716389598 1106 92-93 0.7162299474025708 1106 94-95 0.5020990245480412 1106 96-97 0.5834032520451444 1106 98-99 0.7095122173075268 1106 100-101 0.4227452843615005 1107 1 0.004104492359708445 1107 2 0.0029962885307099896 1107 3 0.008243966940881364 1107 4 0.019316611849411913 1107 5 0.021989993084247317 1107 6 0.07817525933140246 1107 7 0.07124384648630411 1107 8 0.06288497650009361 1107 9 0.0556294862708242 1107 10-11 0.03788946247530589 1107 12-13 0.05870363077968932 1107 14-15 0.1213243635163721 1107 16-17 0.13086328528721225 1107 18-19 0.11900645557542333 1107 20-21 0.17984144571629201 1107 22-23 0.08751130220647241 1107 24-25 0.054598484001083136 1107 26-27 0.17133820560218993 1107 28-29 0.07285824229980875 1107 30-31 0.17931061385982616 1107 32-33 0.17152144323618757 1107 34-35 0.16569300665163667 1107 36-37 0.20603757136456124 1107 38-39 0.1382697363610319 1107 40-41 0.2018458828515861 1107 42-43 0.16702605996830044 1107 44-45 0.19180356241348306 1107 46-47 0.15851663019675044 1107 48-49 0.055041745067647696 1107 50-51 0.03445562441874017 1107 52-53 -0.002451835451310558 1107 54-55 -0.0077942540501041435 1107 56-57 0.10234658019675891 1107 58-59 -0.0037530472605311616 1107 60-61 0.10678141837045985 1107 62-63 0.03879203413107746 1107 64-65 0.12421320926099355 1107 66-67 0.18021568931803955 1107 68-69 0.1626301937757404 1107 70-71 0.13069128053323453 1107 72-73 0.04593899433458404 1107 74-75 0.07251953385289056 1107 76-77 0.15246868870794472 1107 78-79 0.23338528689972549 1107 80-81 0.17205205642569155 1107 82-83 0.08518134976875302 1107 84-85 0.20648911287128868 1107 86-87 0.20967410570873923 1107 88-89 0.3033675469185333 1107 90-91 0.47744282636705293 1107 92-93 0.46581903011156456 1107 94-95 0.3709592015626093 1107 96-97 0.38307276914607513 1107 98-99 0.4762916307794498 1107 100-101 0.16088781586213585 1108 1 -0.042267525464545486 1108 2 0.00471119904962336 1108 3 0.027504522995819514 1108 4 0.020759631417803348 1108 5 0.014280746305921355 1108 6 0.008580596663286144 1108 7 0.029504878372208054 1108 8 0.03324860120684292 1108 9 0.004145970913612018 1108 10-11 0.031796078357594126 1108 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76-77 -0.4114165689379732 2316 78-79 -0.366121541067983 2316 80-81 -0.1501481321527578 2316 82-83 -0.2048324858824202 2316 84-85 -0.31919362235324655 2316 86-87 -0.49633615532223985 2316 88-89 -0.7636248853249334 2316 90-91 -0.8831882221143488 2316 92-93 -0.9091468115859378 2316 94-95 -0.5574533051767325 2316 96-97 -0.8972247219727691 2316 98-99 -0.8561348150091703 2316 100-101 -0.5141294787018289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 17.0 5 53.0 6 108.0 7 271.0 8 602.0 9 1163.0 10 2010.0 11 3244.0 12 5311.0 13 8077.0 14 9156.0 15 12331.0 16 15820.0 17 22773.0 18 22451.0 19 14221.0 20 12670.0 21 12875.0 22 14039.0 23 15848.0 24 17971.0 25 21420.0 26 26066.0 27 31667.0 28 39221.0 29 48511.0 30 60598.0 31 75679.0 32 95004.0 33 124134.0 34 172621.0 35 253961.0 36 426357.0 37 834049.0 38 958157.0 39 118079.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14607357801607 19.77493613520574 13.58116241141917 23.497827875359015 2 15.373421712087149 21.374309948477688 39.5963586910249 23.655909648410265 3 19.610119451686614 24.920841109236967 29.99857329906183 25.47046614001459 4 12.538342587713345 16.542769962882765 37.89838322567878 33.02050422372512 5 13.018185834700589 38.32124472753463 34.80160803002515 13.858961407739622 6 31.583333141572457 36.58248172327065 17.75210035690535 14.082084778251556 7 28.03864288524952 33.05125308063852 21.292591049982857 17.617512984129103 8 25.691662313694717 35.59449132358728 20.501519896725263 18.212326465992742 9 26.03478964215119 16.93634957854794 21.157974913074792 35.870885866226075 10-11 24.50722727576058 27.135564202692784 27.944512263875243 20.412696257671396 12-13 25.27868129111833 24.444535850463332 28.36009644038116 21.91668641803718 14-15 22.094985762303896 25.85874542878556 26.92705803707482 25.119210771835725 16-17 21.565055558911432 29.12923667262095 27.52402566956902 21.781682098898603 18-19 21.274694557147956 28.135316889543244 29.5999008212307 20.990087732078106 20-21 22.258936440473203 27.14297096663591 29.370853650398445 21.227238942492445 22-23 21.741311506343067 27.312089679659618 29.157941546681887 21.788657267315433 24-25 21.6427007680344 27.27891289521728 29.542933096929502 21.535453239818818 26-27 21.616676470545933 27.428790038299848 29.50404356086483 21.450489930289386 28-29 21.27251368597392 27.943519901327523 29.444791851236513 21.339174561462045 30-31 21.587793470580273 27.501625587940747 29.356888501710586 21.55369243976839 32-33 21.298832866587354 27.665715916229644 29.308205371298918 21.727245845884084 34-35 21.978061651299292 27.507396015644687 29.189378274412604 21.325164058643413 36-37 21.64198654580593 27.29277107168449 29.382140284595003 21.68310209791457 38-39 21.4558437328816 27.747186828210662 29.465874252544456 21.33109518636328 40-41 21.748440631215686 27.550915023391255 29.278029945173966 21.422614400219096 42-43 21.37105468123529 27.69159072749855 28.994424215574945 21.94293037569122 44-45 21.63272761529174 27.74607160604093 28.846374285280756 21.774826493386577 46-47 21.681055609760588 27.792741690521993 28.572790774201266 21.95341192551615 48-49 21.79428599864146 27.831569433951465 28.612721792603285 21.761422774803787 50-51 21.458460464894863 28.044300821834984 28.65490625115506 21.842332462115095 52-53 21.81215886614012 28.134361383319867 28.285473858491013 21.768005892049 54-55 21.413601752541023 27.7106454901293 28.649406078206223 22.226346679123456 56-57 21.59966253920551 28.109560852246705 28.409786478096688 21.880990130451092 58-59 21.93398278656022 27.740645777981115 28.416860317025183 21.908511118433477 60-61 21.74499453426155 27.809116276393762 28.71789597836718 21.727993210977502 62-63 21.563307272411116 27.800324214191928 28.641854352146208 21.994514161250752 64-65 21.322794538224418 27.856298100799126 28.514721104355733 22.306186256620723 66-67 21.378245533290425 27.9892130177302 28.226857428455336 22.40568402052403 68-69 21.47127018035592 27.773091458870976 28.35765640715199 22.39798195362112 70-71 21.41651000999122 27.72264412018634 28.380954517716766 22.47989135210567 72-73 21.535432232856003 27.514891191042157 28.466595638437163 22.483080937664674 74-75 21.30767783712138 27.881418140732546 28.171576320446235 22.639327701699845 76-77 21.283048870556993 27.658136146615707 28.32446110817388 22.73435387465342 78-79 21.257106745689715 27.85960805554145 28.190411238074887 22.692873960693948 80-81 21.210638938660473 27.80923399446042 28.162080889140235 22.818046177738875 82-83 21.407269424087325 27.691459367289372 28.11891096131952 22.782360247303778 84-85 21.224333765178834 27.589462202780226 28.058087438358463 23.128116593682478 86-87 21.13788736886133 27.621992350349117 28.2198111991868 23.020309081602754 88-89 21.157529290965833 27.631435672518844 27.938071973531624 23.272963062983703 90-91 21.043736865497202 27.49685147359122 28.168330141729175 23.291081519182402 92-93 21.283142032680654 27.53043820428977 28.038901762435884 23.147518000593692 94-95 20.950154060690018 27.399129942540775 27.997668379474838 23.653047617294376 96-97 20.895948054279067 27.303201562927864 28.162256741737217 23.63859364105585 98-99 20.786194869012824 27.36911458046437 27.817467228936422 24.02722332158638 100-101 20.82435166600221 27.015186999895874 28.312171403291675 23.84828993081024 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 591.0 1 403.0 2 332.5 3 455.5 4 398.5 5 406.5 6 724.0 7 1387.5 8 2114.5 9 2491.0 10 2419.5 11 2343.5 12 2558.0 13 2836.0 14 3056.5 15 3241.0 16 3452.5 17 3675.0 18 3872.0 19 4260.0 20 5037.0 21 6106.0 22 7407.0 23 9405.0 24 11796.5 25 14685.5 26 19639.0 27 26251.0 28 31490.5 29 36932.5 30 44201.5 31 51233.0 32 59150.5 33 67786.0 34 77253.0 35 85360.5 36 94695.5 37 104530.0 38 109020.0 39 111244.0 40 113987.0 41 118788.5 42 123296.5 43 127492.0 44 132305.5 45 135709.5 46 137036.5 47 141633.0 48 158328.0 49 159635.5 50 142282.5 51 129409.0 52 116824.5 53 108923.5 54 102199.5 55 90917.0 56 79925.5 57 69177.5 58 58830.0 59 50305.0 60 41883.0 61 32154.5 62 23589.0 63 17651.0 64 13162.5 65 9900.0 66 7107.0 67 5287.5 68 3903.0 69 2644.0 70 1950.0 71 1483.5 72 1210.5 73 1087.0 74 868.0 75 647.5 76 415.0 77 238.5 78 157.0 79 105.0 80 65.5 81 30.0 82 23.5 83 17.5 84 8.5 85 3.5 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3509799364427743 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011937114704454299 16-17 0.0017833761727136543 18-19 4.314619772694325E-5 20-21 0.0 22-23 0.0 24-25 4.314619772694325E-4 26-27 0.0010642728772646002 28-29 0.0 30-31 0.0033797854886105545 32-33 0.0 34-35 0.01024003092719453 36-37 0.028231995379329862 38-39 0.021860740181651246 40-41 0.013404085427170368 42-43 0.010340705388557397 44-45 0.0028620311158872353 46-47 0.0011505652727184866 48-49 6.184288340861865E-4 50-51 7.191032954490541E-5 52-53 7.191032954490541E-5 54-55 0.0 56-57 0.0 58-59 0.004041360520423684 60-61 0.010124974399922682 62-63 0.004213945311331457 64-65 0.0018265223704405974 66-67 0.004012596388605722 68-69 0.0034660778840644407 70-71 1.4382065908981083E-5 72-73 0.0 74-75 0.0 76-77 0.22073594757104167 78-79 3.883157795424892E-4 80-81 0.0033078751590656487 82-83 5.177543727233189E-4 84-85 0.0 86-87 0.11058370477415554 88-89 0.4115284339195847 90-91 0.39754906585605504 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0013662962613532029 100-101 7.478674272670163E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3476552.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.087239978976605 #Duplication Level Percentage of deduplicated Percentage of total 1 67.82880621776364 31.260424696455736 2 15.857992201473847 14.617021843481293 3 6.591915406447157 9.114095617741297 4 3.3420658850158635 6.161063698731078 5 1.8846230533043777 4.342853746377523 6 1.1563829489323822 3.197669908502601 7 0.7548133745806939 2.4351085593549113 8 0.4973974867049549 1.8338941867768865 9 0.3562766017314569 1.4777824718602732 >10 1.5456195888889568 12.220233223247618 >50 0.10858278170260864 3.4668404727123368 >100 0.06840083917260176 5.973032643239684 >500 0.004561612659439496 1.3771068953317582 >1k 0.0023745380886918237 1.7923585598228462 >5k 1.8746353331777554E-4 0.7305134763640008 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9631 0.2770273535387936 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8169 0.2349741928209329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7566 0.21762942133470173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4953 0.14246874489436662 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.752826363592433E-5 0.0 3 0.0 0.0 0.0 5.752826363592433E-5 0.0 4 0.0 0.0 0.0 1.1505652727184866E-4 0.0 5 0.0 0.0 0.0 1.1505652727184866E-4 0.0 6 0.0 0.0 0.0 1.1505652727184866E-4 0.0 7 0.0 0.0 0.0 1.4382065908981082E-4 0.0 8 0.0 0.0 0.0 1.4382065908981082E-4 0.0 9 0.0 0.0 0.0 1.4382065908981082E-4 0.0 10-11 0.0 0.0 0.0 1.72584790907773E-4 0.0 12-13 0.0 0.0 0.0 1.8696685681675407E-4 0.0 14-15 0.0 0.0 0.0 2.0134892272573516E-4 0.0 16-17 0.0 0.0 0.0 2.3011305454369733E-4 0.0 18-19 0.0 0.0 0.0 2.7325925227064056E-4 0.0 20-21 0.0 0.0 0.0 4.026978454514703E-4 0.0 22-23 0.0 0.0 0.0 5.609005704502621E-4 0.0 24-25 0.0 0.0 0.0 9.348342840837705E-4 0.0 26-27 0.0 0.0 0.0 0.001395060393171165 0.0 28-29 0.0 0.0 0.0 0.00438653010223923 0.0 30-31 0.0 0.0 0.0 0.014094424590801461 0.0 32-33 0.0 0.0 0.0 0.033495831502016946 0.0 34-35 0.0 0.0 0.0 0.0652370509631382 0.0 36-37 0.0 0.0 0.0 0.12368576681723731 0.0 38-39 0.0 0.0 0.0 0.2106828835006639 0.0 40-41 0.0 0.0 0.0 0.3083802572203723 0.0 42-43 0.0 0.0 0.0 0.4106511278991368 0.0 44-45 0.0 0.0 0.0 0.5369256665799907 0.0 46-47 0.0 0.0 0.0 0.6880524151515639 0.0 48-49 0.0 0.0 0.0 0.8517490893275866 0.0 50-51 0.0 0.0 0.0 1.0275554629989714 0.0 52-53 0.0 0.0 0.0 1.2076764564430504 0.0 54-55 0.0 0.0 0.0 1.4067817768869846 0.0 56-57 0.0 0.0 0.0 1.648889474398772 0.0 58-59 0.0 0.0 0.0 1.917273206326268 0.0 60-61 0.0 0.0 0.0 2.207963522478594 0.0 62-63 0.0 0.0 0.0 2.5105334250717375 0.0 64-65 0.0 0.0 0.0 2.8237604385034367 0.0 66-67 0.0 0.0 0.0 3.1840887177870485 0.0 68-69 0.0 0.0 0.0 3.589662976420315 0.0 70-71 0.0 0.0 0.0 4.043028840069126 0.0 72-73 0.0 0.0 0.0 4.508360582554209 0.0 74-75 0.0 0.0 0.0 4.985557529414201 0.0 76-77 0.0 0.0 0.0 5.505397301694322 0.0 78-79 0.0 0.0 0.0 6.095536612137543 0.0 80-81 0.0 1.4382065908981083E-5 0.0 6.727297621321355 0.0 82-83 0.0 2.8764131817962166E-5 0.0 7.3714272071868905 0.0 84-85 0.0 2.8764131817962166E-5 0.0 8.024502438047813 0.0 86-87 0.0 2.8764131817962166E-5 0.0 8.72296746891748 0.0 88-89 0.0 2.8764131817962166E-5 0.0 9.471956697325396 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15040 0.0 26.089869 1 TACATGG 14920 0.0 25.535824 2 ACATGGG 14755 0.0 24.856697 3 GTATCAA 20350 0.0 22.441233 1 TAGCGCA 135 3.8260387E-4 21.088192 9 CATGGGG 5500 0.0 20.876408 4 AGTACAT 10935 0.0 20.262882 2 GAGTACT 10320 0.0 20.140026 12-13 CATGGGA 9770 0.0 20.008097 4 GGTATCA 15805 0.0 19.55432 1 ATCAACG 23185 0.0 19.50244 3 GAGTACA 12745 0.0 19.42925 1 CAACGCA 23265 0.0 19.414984 5 TCAACGC 23305 0.0 19.381659 4 AACGCAG 23820 0.0 19.042292 6 AGTACTT 10650 0.0 19.02584 12-13 TATCAAC 23905 0.0 18.974585 2 GTACTTT 11370 0.0 18.217524 14-15 AGAGTAC 18640 0.0 17.998615 10-11 CTGGACG 1245 0.0 17.53039 4 >>END_MODULE