FastQCFastQC Report
Fri 27 May 2016
SRR939190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3422993
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93130.272071838884859No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75320.2200413497778114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69780.2038566833177865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45900.13409317518323877No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34970.10216205525398388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG144450.027.315411
TACATGG143200.027.0569442
ACATGGG145100.025.6584223
GTATCAA204450.020.924831
CATGGGA96550.020.8502624
ATCCGCG1150.003797374720.5834587
GTGTAGC23650.020.4782711
CATGGGG52150.020.2546794
GTATAGG8900.019.7394921
GAGTACT92250.019.57927312-13
AGTACTT97250.019.01077512-13
AGTACAT104000.018.9920222
ATCAACG230200.018.4570353
TCAACGC230850.018.4050664
CAACGCA231200.018.3152055
GAGTACA121350.018.2726421
ACCGTCC2352.6122307E-618.130968
TATCAAC237500.017.929352
AACGCAG236250.017.9147976
AGAGTAC175000.017.63838810-11
Produced by FastQC (version 0.11.5)