##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3422993 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.280946528374436 34.0 31.0 34.0 31.0 34.0 2 32.37341764940799 34.0 31.0 34.0 31.0 34.0 3 32.45176253647028 34.0 31.0 34.0 31.0 34.0 4 35.874445258871404 37.0 35.0 37.0 35.0 37.0 5 35.91834894199316 37.0 35.0 37.0 35.0 37.0 6 36.00244610491462 37.0 37.0 37.0 35.0 37.0 7 35.94181787692818 37.0 37.0 37.0 35.0 37.0 8 35.945195038377236 37.0 37.0 37.0 35.0 37.0 9 37.656790709183454 39.0 38.0 39.0 35.0 39.0 10-11 37.57177242255535 39.0 38.0 39.0 35.0 39.0 12-13 37.49476613010894 39.0 38.0 39.0 35.0 39.0 14-15 38.83738836158882 40.0 38.0 41.0 35.0 41.0 16-17 38.76457284604439 40.0 38.0 41.0 35.0 41.0 18-19 38.671251445737695 40.0 38.0 41.0 34.5 41.0 20-21 38.66360287619636 40.0 38.5 41.0 34.5 41.0 22-23 38.71042856354074 40.0 39.0 41.0 35.0 41.0 24-25 38.678206178043595 40.5 38.0 41.0 34.0 41.0 26-27 38.590503398633885 40.0 38.0 41.0 34.0 41.0 28-29 38.46854083546183 40.0 38.0 41.0 34.0 41.0 30-31 38.296498707417754 40.0 38.0 41.0 34.0 41.0 32-33 38.130583819481956 40.0 38.0 41.0 34.0 41.0 34-35 37.84881534960779 40.0 38.0 41.0 33.0 41.0 36-37 37.621507113803624 40.0 38.0 41.0 33.0 41.0 38-39 37.4653095989387 40.0 37.5 41.0 32.5 41.0 40-41 37.31226049834166 40.0 37.0 41.0 32.0 41.0 42-43 37.13486223898208 40.0 37.0 41.0 31.0 41.0 44-45 36.90542574875263 40.0 37.0 41.0 31.0 41.0 46-47 36.821054702711926 40.0 37.0 41.0 31.0 41.0 48-49 36.74463839102213 40.0 37.0 41.0 30.0 41.0 50-51 35.648784703912625 39.0 35.5 40.0 29.0 40.5 52-53 35.81036069895556 39.0 35.5 40.0 29.5 40.5 54-55 36.37597243114432 40.0 36.0 41.0 30.0 41.0 56-57 36.31632229455333 40.0 36.0 41.0 30.0 41.0 58-59 36.15199592870917 39.0 36.0 41.0 29.0 41.0 60-61 35.89314117791068 39.0 35.0 41.0 29.0 41.0 62-63 35.55608980211178 39.0 35.0 41.0 28.0 41.0 64-65 35.21304089724986 38.0 35.0 40.0 28.0 41.0 66-67 34.84323748251896 38.0 35.0 40.0 27.5 41.0 68-69 34.439303848999984 37.0 34.0 40.0 26.0 41.0 70-71 34.02308433000009 36.5 34.0 39.0 26.0 41.0 72-73 33.57389015986887 36.0 34.0 39.0 26.0 40.5 74-75 33.125782611883814 35.5 33.5 38.5 25.0 40.0 76-77 32.676461944269235 35.0 33.0 37.0 24.0 39.0 78-79 32.235000480573575 35.0 33.0 37.0 22.5 39.0 80-81 31.7694419766561 35.0 32.0 36.5 21.0 39.0 82-83 31.371632661825483 35.0 32.0 36.0 20.0 37.5 84-85 30.980060286421853 35.0 32.0 35.5 18.0 37.0 86-87 30.575722912667363 35.0 31.0 35.0 13.5 36.5 88-89 30.16212755912735 34.0 31.0 35.0 7.0 36.0 90-91 29.756046097669497 34.0 31.0 35.0 2.0 36.0 92-93 29.350753857808066 34.0 30.0 35.0 2.0 35.5 94-95 28.895333119290633 34.0 29.5 35.0 2.0 35.0 96-97 28.33544079698673 34.0 29.0 35.0 2.0 35.0 98-99 27.673414172918264 34.0 29.0 35.0 2.0 35.0 100-101 24.738557163277868 31.5 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18007353716700436 1101 2 0.269527859663242 1101 3 0.19007108504717252 1101 4 0.2535181853558015 1101 5 0.19992294992090365 1101 6 0.17744591243008045 1101 7 0.17528165944471397 1101 8 0.26375151219876614 1101 9 0.3127629371408389 1101 10-11 0.2883972699293409 1101 12-13 0.2847853866730361 1101 14-15 0.44340721912312375 1101 16-17 0.4301800710535346 1101 18-19 0.4563126308807597 1101 20-21 0.5090969630743558 1101 22-23 0.5050460461786699 1101 24-25 0.5194083835254517 1101 26-27 0.47466438241615805 1101 28-29 0.5040733199695282 1101 30-31 0.542538059946942 1101 32-33 0.5929997329344445 1101 34-35 0.6303327628379805 1101 36-37 0.6698600838273947 1101 38-39 0.6183496952816014 1101 40-41 0.5543197373263453 1101 42-43 0.5932995891354125 1101 44-45 0.5096366102306931 1101 46-47 0.68027727799128 1101 48-49 0.6953419880873142 1101 50-51 0.6849266739458173 1101 52-53 0.7190910062906894 1101 54-55 0.6861575926623757 1101 56-57 0.6744256667831436 1101 58-59 0.6832171387064392 1101 60-61 0.624688388254242 1101 62-63 0.61577653190826 1101 64-65 0.5868636116139356 1101 66-67 0.688571059103765 1101 68-69 0.7341434899826353 1101 70-71 0.5091776403053032 1101 72-73 0.5351160742426231 1101 74-75 0.47771405156269253 1101 76-77 0.3731812230651457 1101 78-79 0.44863184623842045 1101 80-81 0.3305796279212849 1101 82-83 0.2608543215871322 1101 84-85 0.1818251002396103 1101 86-87 0.06552818985343478 1101 88-89 0.18845740431239122 1101 90-91 0.24051479878756865 1101 92-93 0.08210054585187265 1101 94-95 0.04960624766301791 1101 96-97 -0.23332982660044976 1101 98-99 -0.2434768256481874 1101 100-101 -0.2935413382556824 1102 1 0.17015454487414416 1102 2 0.201017562135128 1102 3 0.251502884208314 1102 4 0.161534848568607 1102 5 0.2127539431357235 1102 6 0.2582460410038294 1102 7 0.3088966470333929 1102 8 0.24011254893913048 1102 9 0.3877640566071605 1102 10-11 0.3418190706074071 1102 12-13 0.3950885728651059 1102 14-15 0.3480222667637918 1102 16-17 0.2890367158411422 1102 18-19 0.34084202285954746 1102 20-21 0.42273022313037956 1102 22-23 0.4393472579445543 1102 24-25 0.43907374466876803 1102 26-27 0.4284443081807794 1102 28-29 0.34018605387290535 1102 30-31 0.41663350512484953 1102 32-33 0.34945037557753267 1102 34-35 0.3521059836068048 1102 36-37 0.29572819090876834 1102 38-39 0.22283495425321576 1102 40-41 0.14918695820694694 1102 42-43 0.22995275625315514 1102 44-45 0.21574476560359557 1102 46-47 0.353324902692016 1102 48-49 0.38466335545381725 1102 50-51 0.30664477184583205 1102 52-53 0.20366285120937988 1102 54-55 0.22125312219078097 1102 56-57 0.2527187114781384 1102 58-59 0.2246292352603163 1102 60-61 0.2786400002336791 1102 62-63 0.16818543251596907 1102 64-65 0.1743104196075791 1102 66-67 0.18665842051991177 1102 68-69 0.11985153247167801 1102 70-71 0.0040543028863524455 1102 72-73 0.0299755543173319 1102 74-75 -0.08477646768570679 1102 76-77 -0.09066522156188483 1102 78-79 -0.19909761572002793 1102 80-81 -0.15531665847047904 1102 82-83 -0.344227465407414 1102 84-85 -0.40085729380073687 1102 86-87 -0.4017372264127417 1102 88-89 -0.4225821998026653 1102 90-91 -0.4597219726785049 1102 92-93 -0.6064338974366557 1102 94-95 -0.5960804052090438 1102 96-97 -0.6768906736737144 1102 98-99 -0.7546694061378183 1102 100-101 -0.8954974711434538 1103 1 0.2183782593006569 1103 2 0.25699310279843957 1103 3 0.25909647570118466 1103 4 0.18928761348608703 1103 5 0.23933246321622903 1103 6 0.2659817755717526 1103 7 0.2316817796723285 1103 8 0.34933139898824805 1103 9 0.3637282437041023 1103 10-11 0.3423400383871922 1103 12-13 0.38091158909198697 1103 14-15 0.4435875033951291 1103 16-17 0.4773774077605637 1103 18-19 0.5160514101133771 1103 20-21 0.45383854799882073 1103 22-23 0.5140779739232499 1103 24-25 0.49556365999752927 1103 26-27 0.5097647301847985 1103 28-29 0.48755819494767394 1103 30-31 0.5323101989126826 1103 32-33 0.5311380563992856 1103 34-35 0.3845242150206474 1103 36-37 0.3164205684863717 1103 38-39 0.3957199334625514 1103 40-41 0.35361071442709857 1103 42-43 0.3398076742135885 1103 44-45 0.388289752434126 1103 46-47 0.49736411272517245 1103 48-49 0.5176907235831507 1103 50-51 0.4826735491136702 1103 52-53 0.43131090853147924 1103 54-55 0.3890490552449606 1103 56-57 0.48876835204166014 1103 58-59 0.5336584494616616 1103 60-61 0.42402599355339987 1103 62-63 0.3848850647542861 1103 64-65 0.4166022308478361 1103 66-67 0.4429117100928437 1103 68-69 0.38682712565641 1103 70-71 0.3551891007850472 1103 72-73 0.2760899750228418 1103 74-75 0.148866853768844 1103 76-77 0.31472660498780414 1103 78-79 0.2630833306265501 1103 80-81 0.19953827625210252 1103 82-83 0.10816862878695943 1103 84-85 -0.04420137726285489 1103 86-87 -0.028753741605402894 1103 88-89 -0.13023501757680833 1103 90-91 -0.030540289914579688 1103 92-93 -0.007826106163481228 1103 94-95 -0.08838590554261216 1103 96-97 -0.00637552794912466 1103 98-99 -0.0862503120317264 1103 100-101 -0.2894081713210177 1104 1 0.12733482065500823 1104 2 0.27721139198541067 1104 3 0.2633726486993453 1104 4 0.2576133324135128 1104 5 0.2779890757153112 1104 6 0.22413764238913103 1104 7 0.2434042399491929 1104 8 0.23929177255973855 1104 9 0.248447355934573 1104 10-11 0.3214579420545718 1104 12-13 0.34123509031473276 1104 14-15 0.4117482912819952 1104 16-17 0.42400797942121926 1104 18-19 0.4658660641557475 1104 20-21 0.43461521740180586 1104 22-23 0.5081323979432213 1104 24-25 0.4591921214004415 1104 26-27 0.4380607722696155 1104 28-29 0.42487913463556026 1104 30-31 0.39268097678549907 1104 32-33 0.42784545261629603 1104 34-35 0.49786912397011207 1104 36-37 0.40116858394153354 1104 38-39 0.24423867005855016 1104 40-41 0.3434943708891751 1104 42-43 0.3612806212250561 1104 44-45 0.37798462463674554 1104 46-47 0.5319279641389585 1104 48-49 0.460732137928602 1104 50-51 0.4492381053793295 1104 52-53 0.5506722009891334 1104 54-55 0.6531114948060619 1104 56-57 0.5922883135326771 1104 58-59 0.5394755189110683 1104 60-61 0.6272887270893079 1104 62-63 0.652815701312889 1104 64-65 0.6116596719776624 1104 66-67 0.6284993158743504 1104 68-69 0.4451197724181739 1104 70-71 0.5344463631231733 1104 72-73 0.5792852318854784 1104 74-75 0.48669569510411037 1104 76-77 0.5330568366863702 1104 78-79 0.5168702977434876 1104 80-81 0.43526137261480713 1104 82-83 0.5011910250254559 1104 84-85 0.4553940850383107 1104 86-87 0.5318792067813938 1104 88-89 0.532824404874443 1104 90-91 0.5858060416322708 1104 92-93 0.4369415505908485 1104 94-95 0.3099070850493355 1104 96-97 0.5236864044624561 1104 98-99 0.3823759359643901 1104 100-101 0.1556119857234144 1105 1 0.1396512445757665 1105 2 0.20502543271688012 1105 3 0.16967486038426927 1105 4 0.14626625249673708 1105 5 0.17166743452015965 1105 6 0.25032526095422725 1105 7 0.17121582124898538 1105 8 0.19010928764835455 1105 9 0.27130080495957287 1105 10-11 0.26585449589890686 1105 12-13 0.1942047310901458 1105 14-15 0.2522475887043498 1105 16-17 0.2194070386066258 1105 18-19 0.29522823514527374 1105 20-21 0.2424292827275849 1105 22-23 0.2266115017919077 1105 24-25 0.23359931638582765 1105 26-27 0.25671416784296497 1105 28-29 0.3031694443107966 1105 30-31 0.4698542535846997 1105 32-33 0.42722608573959064 1105 34-35 0.3609474388331222 1105 36-37 0.3652395664189001 1105 38-39 0.4715395503711264 1105 40-41 0.34678345084858364 1105 42-43 0.24284013045366493 1105 44-45 0.21848904587508855 1105 46-47 0.2791086356293917 1105 48-49 0.3732390858230801 1105 50-51 0.2956398338679094 1105 52-53 0.2343284167108024 1105 54-55 0.3065435497268183 1105 56-57 0.2404616936280064 1105 58-59 0.3124847319421207 1105 60-61 0.2589988765079809 1105 62-63 0.2725611255402569 1105 64-65 0.3537763736502413 1105 66-67 0.351522424694366 1105 68-69 0.28105170026828574 1105 70-71 0.11092136627944882 1105 72-73 0.1623849192097353 1105 74-75 0.1717298074214355 1105 76-77 0.13665793654421776 1105 78-79 0.19760819283069253 1105 80-81 0.2972768030891366 1105 82-83 0.29652408946092734 1105 84-85 0.29240631825146224 1105 86-87 0.26614970655430525 1105 88-89 0.3671499139358083 1105 90-91 0.3680248015212335 1105 92-93 0.2945880056480128 1105 94-95 0.3503610469478211 1105 96-97 0.44356078489062867 1105 98-99 0.5051856071055845 1105 100-101 0.23786214435675745 1106 1 0.0874999956236806 1106 2 0.1624472316687715 1106 3 0.21539262542155768 1106 4 0.2582583380301884 1106 5 0.19232093424800212 1106 6 0.20406278037287962 1106 7 0.2025979525131163 1106 8 0.15566347909739164 1106 9 0.24775328041558708 1106 10-11 0.2962694690207073 1106 12-13 0.3222513239361362 1106 14-15 0.4021300835094834 1106 16-17 0.3722612328368484 1106 18-19 0.3524832621426697 1106 20-21 0.3805801303939731 1106 22-23 0.37027466416163435 1106 24-25 0.3594754899478474 1106 26-27 0.32844905307923256 1106 28-29 0.313180727988005 1106 30-31 0.3924790196669079 1106 32-33 0.3481747887126829 1106 34-35 0.2308666055891777 1106 36-37 0.29905056879881187 1106 38-39 0.31406838533145987 1106 40-41 0.5358595401803399 1106 42-43 0.5160405031328565 1106 44-45 0.35808323080819804 1106 46-47 0.1911169116503899 1106 48-49 0.2733567896742315 1106 50-51 0.3584215781495885 1106 52-53 0.38117603939099354 1106 54-55 0.37572460904161176 1106 56-57 0.34777542355875823 1106 58-59 0.40324155280580243 1106 60-61 0.43610224929444996 1106 62-63 0.3686700448103224 1106 64-65 0.32401170189002926 1106 66-67 0.31814161250798634 1106 68-69 0.2787047748716702 1106 70-71 0.2772451521915329 1106 72-73 0.4283239078903023 1106 74-75 0.3787354214481553 1106 76-77 0.3651619354953084 1106 78-79 0.3906950533064375 1106 80-81 0.3617603364693487 1106 82-83 0.4308979844353189 1106 84-85 0.4243392240764585 1106 86-87 0.4765405230713817 1106 88-89 0.4676102005571039 1106 90-91 0.2707584113841186 1106 92-93 0.41356653487631334 1106 94-95 0.5262174542091032 1106 96-97 0.4748281921479389 1106 98-99 0.5333827814185277 1106 100-101 0.4978630979069436 1107 1 0.059600500951297875 1107 2 0.14486685695126766 1107 3 0.10143779411349385 1107 4 0.08820350787321729 1107 5 0.12865752004434228 1107 6 0.1415281997717841 1107 7 0.1895415453656284 1107 8 0.24874563224580726 1107 9 0.2507714330728774 1107 10-11 0.20825191109221208 1107 12-13 0.2823454061928814 1107 14-15 0.3161832369149806 1107 16-17 0.2824132531880892 1107 18-19 0.2737100917010764 1107 20-21 0.3204454722862806 1107 22-23 0.34715630929656527 1107 24-25 0.3094690630400194 1107 26-27 0.36289027660900075 1107 28-29 0.3464845806794372 1107 30-31 0.37004843986390057 1107 32-33 0.35436764199116766 1107 34-35 0.3130563010496701 1107 36-37 0.3622065450556988 1107 38-39 0.33691594675159564 1107 40-41 0.29118284242150594 1107 42-43 0.32855570708646553 1107 44-45 0.34826090123636533 1107 46-47 0.37144174867551527 1107 48-49 0.4548869117279324 1107 50-51 0.2964827814969553 1107 52-53 0.4191934054714963 1107 54-55 0.32152850969504243 1107 56-57 0.358964190474957 1107 58-59 0.32388401337144046 1107 60-61 0.23446051007726965 1107 62-63 0.3072360002325141 1107 64-65 0.31654977412463836 1107 66-67 0.25464453051599634 1107 68-69 0.322086786436266 1107 70-71 0.2996243167318866 1107 72-73 0.3607944688154703 1107 74-75 0.26244877368491615 1107 76-77 0.35868533903221334 1107 78-79 0.3740074432336442 1107 80-81 0.3714783007319049 1107 82-83 0.3989712166519723 1107 84-85 0.3374868549455705 1107 86-87 0.28374844409139754 1107 88-89 0.3727069303161805 1107 90-91 0.4192673687670059 1107 92-93 0.3052137609461525 1107 94-95 0.36417573110715296 1107 96-97 0.3245477103792531 1107 98-99 0.2604711427680648 1107 100-101 0.3886212044439752 1108 1 0.11178215937879088 1108 2 0.1742937597474281 1108 3 0.12855990233988024 1108 4 0.1509426807565859 1108 5 0.2424378401540892 1108 6 0.21976876083916608 1108 7 0.12492381840500144 1108 8 0.2366176804889335 1108 9 0.33492232626139185 1108 10-11 0.20398370133545285 1108 12-13 0.20821383664381443 1108 14-15 0.22284159069422316 1108 16-17 0.1940320198585468 1108 18-19 0.22216097563871529 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-1.0840448693251048 2316 46-47 -1.2600385429936054 2316 48-49 -1.2240471148151286 2316 50-51 -1.3618897759500683 2316 52-53 -1.1833516367635823 2316 54-55 -1.1489856651228578 2316 56-57 -1.114200497489989 2316 58-59 -0.9107151174691026 2316 60-61 -0.7547576448797884 2316 62-63 -1.0189843900043556 2316 64-65 -1.2583382832594552 2316 66-67 -1.2251519364557737 2316 68-69 -1.2032897128418156 2316 70-71 -1.1748786124355135 2316 72-73 -1.4732810036189221 2316 74-75 -1.11013457389258 2316 76-77 -1.2043757137312667 2316 78-79 -0.8271856724122699 2316 80-81 -0.8652683778137522 2316 82-83 -0.9275425987184214 2316 84-85 -0.9382982135830424 2316 86-87 -1.0376854271857248 2316 88-89 -1.1328839486209112 2316 90-91 -1.2851300647558155 2316 92-93 -1.3444022100650095 2316 94-95 -1.31418003288643 2316 96-97 -1.3595741906913368 2316 98-99 -1.7469883548078649 2316 100-101 -1.7129015558930298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7881.0 3 1648.0 4 1645.0 5 1895.0 6 2277.0 7 2713.0 8 3345.0 9 4293.0 10 5380.0 11 7240.0 12 10628.0 13 18367.0 14 14969.0 15 14445.0 16 17669.0 17 24973.0 18 21003.0 19 14294.0 20 14265.0 21 15213.0 22 16657.0 23 18577.0 24 21148.0 25 24769.0 26 28720.0 27 33130.0 28 39863.0 29 47815.0 30 58072.0 31 72146.0 32 91755.0 33 119832.0 34 166223.0 35 247182.0 36 413164.0 37 756840.0 38 888143.0 39 174758.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85935170550018 19.33793796042727 13.166214445709876 23.636495888362674 2 15.10567134318361 20.933148671119426 40.073445641888874 23.887734343808088 3 19.63077113877058 24.768098523429703 29.854940372370315 25.7461899654294 4 12.338767524505933 16.208697982304944 38.18894228687708 33.26359220631204 5 13.10705770496599 38.1713742412053 34.82878665984056 13.892781393988152 6 31.59748017260005 36.233555617731646 18.011877582488328 14.157086627179977 7 28.061505695399557 32.953201438228376 21.426409800350346 17.558883066021725 8 25.67611859958066 35.26415677454799 20.725191051729645 18.334533574141695 9 26.166129804241358 16.84062919477069 21.27989717503255 35.71334382595541 10-11 24.496186155548017 26.919381159753137 28.12191496114377 20.46251772355508 12-13 25.31202061702241 24.358623678085333 28.39159185008917 21.937763854803087 14-15 22.220001836082293 25.732167050743115 26.931848016826464 25.115983096348128 16-17 21.630059444493742 29.004492216138033 27.544013967402087 21.821434371966138 18-19 21.332163901547254 28.050412081425318 29.574216397611096 21.04320761941633 20-21 22.291428725148084 27.150011783770445 29.2918070923678 21.266752398713667 22-23 21.818289220461228 27.286839772733586 29.077020434267293 21.817850572537893 24-25 21.707282761194442 27.173616438572513 29.488767460952776 21.630333339280266 26-27 21.650452270654014 27.35593277842789 29.440425658239743 21.553189292678354 28-29 21.298665330334874 27.890038064691698 29.337259660975512 21.47403694399792 30-31 21.618199036832237 27.51617963632865 29.227581098494337 21.638040228344774 32-33 21.35242134296146 27.605712015676215 29.23706689775883 21.804799743603503 34-35 21.9950395471597 27.489339834113146 29.09335932238121 21.422261296345944 36-37 21.717595233325834 27.232763072021164 29.287900798851453 21.76174089580155 38-39 21.472201127542448 27.716999649368248 29.3578182364926 21.452980986596703 40-41 21.740299007423587 27.48543737347656 29.238736065449363 21.53552755365049 42-43 21.428238959385293 27.623484457044572 28.951211164269125 21.997065419301013 44-45 21.6719185970613 27.680646801697005 28.809903614999214 21.83753098624248 46-47 21.71527584029603 27.748476527510608 28.51294400963275 22.023303622560608 48-49 21.779879500450246 27.779172856410284 28.60413424944476 21.836813393694715 50-51 21.449961040948267 28.013893094482384 28.662628122120843 21.87351774244851 52-53 21.786110826797756 28.07487585133086 28.345119569959614 21.79389375191177 54-55 21.444052481524682 27.650786294846174 28.656121258682603 22.249039964946533 56-57 21.629057902626045 28.059430640349415 28.382957465889053 21.92855399113549 58-59 21.940549419757158 27.668954875937786 28.477380136159386 21.91311556814567 60-61 21.756111773399407 27.665800035619796 28.83098405999383 21.747104130986962 62-63 21.563887003519632 27.671396949240084 28.811956035489082 21.952760011751195 64-65 21.36455988985773 27.718946305644792 28.671060119320792 22.245433685176685 66-67 21.387847205240245 27.88607322202556 28.362640737445933 22.363438835288264 68-69 21.490888279962146 27.68732503026762 28.481965613168565 22.33982107660167 70-71 21.417335649637756 27.601263283877092 28.561329065590908 22.420072000894244 72-73 21.541483603433452 27.310731036386958 28.715687347212725 22.43209801296686 74-75 21.28247811390843 27.658914482757407 28.50946338649209 22.549144016842078 76-77 21.30418866718246 27.383973908767317 28.64946212749387 22.662375296556355 78-79 21.333141709834557 27.529323753528534 28.559158152363878 22.578376384273035 80-81 21.247454489853414 27.49945202989707 28.524629586157417 22.728463894092098 82-83 21.451131984907555 27.327140107001497 28.61044152176055 22.611286386330402 84-85 21.291848009693748 27.17120147197462 28.574023118822574 22.96292739950906 86-87 21.168285745664136 27.14959385499763 28.819483801477286 22.86263659786095 88-89 21.24726661454904 27.10591820900186 28.57225505841501 23.07456011803409 90-91 21.191273341854323 27.0596103695264 28.780360069889166 22.968756218730114 92-93 21.423922876033753 26.97345742594151 28.735471804582787 22.86714789344196 94-95 21.123513195293096 26.738597155727117 28.779959879459206 23.35792976952058 96-97 21.134008581472216 26.565538552370406 28.94281050191258 23.357642364244796 98-99 21.039449748517143 26.58731755633213 28.69413087213133 23.6791018230194 100-101 21.198555502923618 26.090883381558612 29.247908859364752 23.462652256153017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3072.0 1 1714.0 2 447.0 3 579.0 4 536.5 5 512.5 6 904.0 7 1654.5 8 2463.0 9 3004.5 10 3055.5 11 3130.0 12 3498.0 13 3795.5 14 4073.5 15 4371.0 16 4614.5 17 4796.0 18 4897.5 19 5181.5 20 5604.0 21 6341.0 22 7486.0 23 9180.0 24 11413.0 25 14222.5 26 19261.5 27 25798.0 28 30720.5 29 36047.0 30 43160.5 31 49929.5 32 57499.5 33 66289.0 34 75405.5 35 83332.0 36 93147.5 37 102974.5 38 106643.5 39 108764.0 40 111873.0 41 116946.0 42 121778.5 43 125873.0 44 130367.0 45 133155.0 46 134549.0 47 138657.0 48 154667.0 49 155438.0 50 137661.0 51 125485.5 52 113855.0 53 106023.5 54 99369.5 55 88891.5 56 78167.0 57 67403.0 58 57337.5 59 49300.0 60 41337.0 61 31944.5 62 23663.0 63 17835.0 64 13423.5 65 10154.5 66 7423.5 67 5659.0 68 4197.0 69 2885.5 70 2229.0 71 1725.5 72 1367.5 73 1115.0 74 879.0 75 710.0 76 504.5 77 326.5 78 238.0 79 177.5 80 106.5 81 70.0 82 61.5 83 46.5 84 29.5 85 20.5 86 16.5 87 13.0 88 9.5 89 9.5 90 6.0 91 8.0 92 8.0 93 4.0 94 2.5 95 1.0 96 0.5 97 1.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39509283250067995 2 0.12088835706061916 3 0.1224659238274808 4 0.13546624255439613 5 0.1385629476893467 6 0.07595691840444897 7 0.08624031658843591 8 0.0831436114534853 9 0.0821503286743502 10-11 0.08052894060840908 12-13 0.08491107051635806 14-15 0.07791426976333285 16-17 0.08014915601638684 18-19 0.09052019679853274 20-21 0.08866509513750101 22-23 0.09915299271719222 24-25 0.1128836664287657 26-27 0.11428594799930938 28-29 0.10758128924014743 30-31 0.09756081885063743 32-33 0.0961001088813211 34-35 0.3450927302509821 36-37 0.5362266297360234 38-39 0.5174126853312292 40-41 0.48952773201698047 42-43 0.5181576474155805 44-45 0.5274477628204324 46-47 0.5241903795888568 48-49 0.5307343602513941 50-51 0.5298287200704179 52-53 0.5288792585903623 54-55 0.5231970968097218 56-57 0.4948447163052919 58-59 0.48546695830228104 60-61 0.4316690101323608 62-63 0.507815820832821 64-65 0.5347805268664002 66-67 0.5448009972559102 68-69 0.560620486223606 70-71 0.5552888948356015 72-73 0.5417919347191187 74-75 0.5451953889476256 76-77 0.5218970649370303 78-79 0.49688971026233475 80-81 0.5036966187193489 82-83 0.517485720829695 84-85 0.5112338821610211 86-87 0.5129721270245075 88-89 0.5222038140305867 90-91 0.5392795135718945 92-93 0.5465246350197035 94-95 0.5405357241455065 96-97 0.5423762187068452 98-99 0.5429751097942649 100-101 0.5198374638802943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3422993.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.35498289420111 #Duplication Level Percentage of deduplicated Percentage of total 1 68.91783493287153 32.63602894351507 2 15.592443479586102 14.767597885091952 3 6.234123146144275 8.856503848380159 4 3.176264534260779 6.0164781074950735 5 1.7729833829509247 4.197979888567192 6 1.1183808182430466 3.1776542710261255 7 0.7168754792677678 2.3763338240598157 8 0.47935581893318935 1.815990928465356 9 0.34430121279332354 1.4673940238052479 >10 1.4785418232531318 11.99616539321569 >50 0.09785974184325649 3.2354040100454027 >100 0.06460645708977296 5.777530514792999 >500 0.0040182329795977285 1.2825683542665938 >1k 0.0022254828768737732 1.6989668789274097 >5k 1.8545690640614775E-4 0.6974031283459866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9313 0.272071838884859 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7532 0.2200413497778114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6978 0.2038566833177865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4590 0.13409317518323877 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3497 0.10216205525398388 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.921419938632653E-5 0.0 3 0.0 0.0 0.0 5.842839877265306E-5 0.0 4 0.0 0.0 0.0 8.764259815897958E-5 0.0 5 0.0 0.0 0.0 1.1685679754530612E-4 0.0 6 0.0 0.0 0.0 1.1685679754530612E-4 0.0 7 0.0 0.0 0.0 1.7528519631795916E-4 0.0 8 0.0 0.0 0.0 1.7528519631795916E-4 0.0 9 0.0 0.0 0.0 2.0449939570428569E-4 0.0 10-11 0.0 0.0 0.0 2.3371359509061223E-4 0.0 12-13 0.0 0.0 0.0 2.6292779447693876E-4 0.0 14-15 0.0 0.0 0.0 2.6292779447693876E-4 0.0 16-17 0.0 0.0 0.0 3.651774923290816E-4 0.0 18-19 0.0 0.0 0.0 4.0899879140857137E-4 0.0 20-21 0.0 0.0 0.0 4.6742719018122447E-4 0.0 22-23 0.0 0.0 0.0 7.157478849649999E-4 0.0 24-25 0.0 0.0 0.0 0.0012269963742257142 0.0 26-27 0.0 0.0 0.0 0.0017528519631795918 0.0 28-29 0.0 0.0 0.0 0.005127091992300306 0.0 30-31 0.0 0.0 0.0 0.014475635795924795 0.0 32-33 0.0 0.0 0.0 0.03458961207341061 0.0 34-35 0.0 0.0 0.0 0.06697355209315356 0.0 36-37 0.0 0.0 0.0 0.12668737563880497 0.0 38-39 0.0 0.0 0.0 0.21736825053396253 0.0 40-41 0.0 0.0 0.0 0.31492906938459997 0.0 42-43 0.0 0.0 0.0 0.41795294352048046 0.0 44-45 0.0 0.0 0.0 0.5460864220289086 0.0 46-47 0.0 0.0 0.0 0.6944069123132883 0.0 48-49 0.0 0.0 0.0 0.8593941033475674 0.0 50-51 0.0 0.0 0.0 1.037206327912444 0.0 52-53 0.0 0.0 0.0 1.2186265061015316 0.0 54-55 0.0 0.0 0.0 1.4223371184223863 0.0 56-57 0.0 0.0 0.0 1.669766780124879 0.0 58-59 0.0 0.0 0.0 1.9397059824545362 0.0 60-61 0.0 0.0 0.0 2.2342727548668666 0.0 62-63 0.0 0.0 0.0 2.5314834123236594 0.0 64-65 0.0 0.0 0.0 2.8427460996852756 0.0 66-67 0.0 0.0 0.0 3.2009852196601045 0.0 68-69 0.0 0.0 0.0 3.6139133208861365 0.0 70-71 0.0 0.0 0.0 4.066397448081255 0.0 72-73 0.0 0.0 0.0 4.522956956090766 0.0 74-75 0.0 0.0 0.0 4.992531389926886 0.0 76-77 0.0 0.0 0.0 5.50405741408177 2.921419938632653E-5 78-79 0.0 0.0 0.0 6.086004265857394 2.921419938632653E-5 80-81 0.0 0.0 0.0 6.72710987139033 2.921419938632653E-5 82-83 0.0 0.0 0.0 7.3717065737499325 2.921419938632653E-5 84-85 0.0 0.0 0.0 8.0276968138702 2.921419938632653E-5 86-87 0.0 0.0 0.0 8.726237535396653 2.921419938632653E-5 88-89 0.0 0.0 0.0 9.47553792835685 2.921419938632653E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14445 0.0 27.31541 1 TACATGG 14320 0.0 27.056944 2 ACATGGG 14510 0.0 25.658422 3 GTATCAA 20445 0.0 20.92483 1 CATGGGA 9655 0.0 20.850262 4 ATCCGCG 115 0.0037973747 20.583458 7 GTGTAGC 2365 0.0 20.478271 1 CATGGGG 5215 0.0 20.254679 4 GTATAGG 890 0.0 19.739492 1 GAGTACT 9225 0.0 19.579273 12-13 AGTACTT 9725 0.0 19.010775 12-13 AGTACAT 10400 0.0 18.992022 2 ATCAACG 23020 0.0 18.457035 3 TCAACGC 23085 0.0 18.405066 4 CAACGCA 23120 0.0 18.315205 5 GAGTACA 12135 0.0 18.272642 1 ACCGTCC 235 2.6122307E-6 18.13096 8 TATCAAC 23750 0.0 17.92935 2 AACGCAG 23625 0.0 17.914797 6 AGAGTAC 17500 0.0 17.638388 10-11 >>END_MODULE