FastQCFastQC Report
Fri 27 May 2016
SRR939190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3422993
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92510.2702605585229067No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80240.23441473587588407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75920.221794201740991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48140.1406371558457759No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG145600.028.048091
TACATGG144100.027.562642
ACATGGG144950.026.3861583
CATGGGG55350.021.6901344
CATGGGA92950.021.0843374
AGTACAT103800.019.8861732
GAGTACT99500.019.62627212-13
GTATCAA211200.019.471561
GAGTACA122550.018.9753741
GGTATCA169100.018.2606091
GTGTAGC20950.018.1965831
GTACTTT108950.018.0546214-15
ACCGTCC2403.1233849E-617.7946978
AGTACTT101150.017.73441912-13
ATCAACG234600.017.2738533
CAACGCA235150.017.193095
TCAACGC236350.017.1459524
AACGCAG240300.016.8838586
TATCAAC241950.016.866782
GTGTAGG15450.016.6551721
Produced by FastQC (version 0.11.5)