FastQCFastQC Report
Fri 27 May 2016
SRR939189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417680
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71100.29408358426259884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53710.2221551239204527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34070.1409202210383508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.039.116241
GTATCAA79250.037.857841
ATCAACG96450.031.0447753
TATCAAC98100.030.5709112
TCAACGC98950.030.2604224
CAACGCA99500.029.9979215
AACGCAG102400.028.9068576
GTACATG83700.025.3524591
ACGCAGA119300.024.5340277
TACATGG83300.024.2860322
CGCAGAG120900.024.2480148
ACATGGG87000.021.9462093
GCAGAGT132100.021.7256419
GAGTACT68800.021.13390412-13
TACCTGG20400.020.9019132
GTACCTG22400.020.5570951
GTACTTT71950.020.07783314-15
GTATAGG5750.019.8144251
GTACGCT1200.00483659619.7406963
TAGCGCG1250.006123543318.9510694
Produced by FastQC (version 0.11.5)