##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2417680 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07711318288607 33.0 31.0 34.0 30.0 34.0 2 32.18110916250289 34.0 31.0 34.0 30.0 34.0 3 32.24429742563118 34.0 31.0 34.0 30.0 34.0 4 35.678675837993445 37.0 35.0 37.0 35.0 37.0 5 35.71692200787532 37.0 35.0 37.0 35.0 37.0 6 35.79505186790642 37.0 36.0 37.0 35.0 37.0 7 35.71888091062506 37.0 36.0 37.0 35.0 37.0 8 35.70861693855266 37.0 36.0 37.0 35.0 37.0 9 37.39434416465372 39.0 38.0 39.0 35.0 39.0 10-11 37.30352631448331 39.0 37.5 39.0 34.0 39.0 12-13 37.206580688924916 39.0 37.0 39.0 34.0 39.0 14-15 38.5289360047649 40.0 38.0 41.0 34.0 41.0 16-17 38.45216323086595 40.0 38.0 41.0 34.0 41.0 18-19 38.370101502266635 40.0 38.0 41.0 34.0 41.0 20-21 38.265575469044705 40.0 38.0 41.0 34.0 41.0 22-23 38.18072842559809 40.0 38.0 41.0 33.5 41.0 24-25 38.23293157076205 40.0 38.0 41.0 34.0 41.0 26-27 38.15300784222892 40.0 38.0 41.0 33.5 41.0 28-29 38.03087753548857 40.0 38.0 41.0 33.0 41.0 30-31 37.87288640349426 40.0 38.0 41.0 33.0 41.0 32-33 37.681446262532674 40.0 38.0 41.0 33.0 41.0 34-35 37.37580200853711 40.0 37.0 41.0 31.5 41.0 36-37 37.129585594454184 40.0 37.0 41.0 31.0 41.0 38-39 36.898265279110554 40.0 36.5 41.0 30.5 41.0 40-41 36.75036419211806 40.0 36.5 41.0 30.0 41.0 42-43 36.56597709374276 40.0 36.0 41.0 30.0 41.0 44-45 36.31619258959002 39.5 36.0 41.0 30.0 41.0 46-47 35.983194426061345 39.0 35.0 41.0 28.5 41.0 48-49 35.98153622481056 39.0 36.0 41.0 28.0 41.0 50-51 34.999977044108405 38.0 34.5 40.0 26.5 40.5 52-53 35.16788615532246 38.5 35.0 40.0 27.5 40.5 54-55 35.674446783693455 39.0 35.0 41.0 28.0 41.0 56-57 35.558572888057974 39.0 35.0 41.0 27.0 41.0 58-59 35.36834506634459 39.0 35.0 41.0 27.0 41.0 60-61 35.078384856556696 38.5 35.0 41.0 26.0 41.0 62-63 34.70926011713709 38.0 34.5 40.0 26.0 41.0 64-65 34.33398588729691 37.5 34.0 40.0 25.5 41.0 66-67 33.947125756923995 37.0 34.0 40.0 24.5 41.0 68-69 33.53811070944046 36.0 34.0 39.0 23.0 41.0 70-71 33.138072656430964 36.0 33.0 39.0 22.0 41.0 72-73 32.68633462658416 35.0 33.0 38.5 21.5 40.0 74-75 32.22542333973065 35.0 32.5 37.5 20.0 39.5 76-77 31.78667441514179 35.0 32.0 37.0 18.5 39.0 78-79 31.36580792329837 35.0 32.0 36.5 17.5 39.0 80-81 30.94812671652162 35.0 31.0 36.0 13.5 37.5 82-83 30.5345266950134 35.0 31.0 35.5 7.5 37.0 84-85 30.16463841368585 34.5 31.0 35.0 6.0 37.0 86-87 29.778269663479037 34.0 30.5 35.0 2.0 36.0 88-89 29.387020614804275 34.0 30.0 35.0 2.0 36.0 90-91 29.021661882465835 34.0 29.5 35.0 2.0 36.0 92-93 28.62046941696172 34.0 29.0 35.0 2.0 35.0 94-95 28.174603545547797 34.0 29.0 35.0 2.0 35.0 96-97 27.625310835180834 34.0 28.0 35.0 2.0 35.0 98-99 26.930371265014394 34.0 26.5 35.0 2.0 35.0 100-101 24.3489212799047 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31572919045015624 1101 2 0.3268962203856134 1101 3 0.31039887209029615 1101 4 0.17468855163204466 1101 5 0.26915173222636923 1101 6 0.2773017983107522 1101 7 0.14907384193233497 1101 8 0.3323845501358207 1101 9 0.3601959399989667 1101 10-11 0.3575634628524895 1101 12-13 0.3178707257097315 1101 14-15 0.3491581685479659 1101 16-17 0.47861510429216025 1101 18-19 0.4752806621862007 1101 20-21 0.43887698639435513 1101 22-23 0.43253435250843353 1101 24-25 0.5458244778677823 1101 26-27 0.4419990061158501 1101 28-29 0.4938263933449605 1101 30-31 0.44456476346931595 1101 32-33 0.4859544162722287 1101 34-35 0.41554258623622076 1101 36-37 0.30125945127937115 1101 38-39 0.3766842912277184 1101 40-41 0.2388522532681563 1101 42-43 0.245789132801427 1101 44-45 0.3288701540087686 1101 46-47 0.12423468625769374 1101 48-49 0.248778225479505 1101 50-51 0.2150326781679368 1101 52-53 0.3550579649707615 1101 54-55 0.22781780250041805 1101 56-57 0.22085775295727927 1101 58-59 0.32744386819558 1101 60-61 0.25983509480477807 1101 62-63 0.18220989634110651 1101 64-65 0.2571120763257966 1101 66-67 0.3005325657257245 1101 68-69 0.2504637082914343 1101 70-71 0.2528798194727031 1101 72-73 0.05230211877798041 1101 74-75 0.038720495373603114 1101 76-77 0.015354867986559384 1101 78-79 0.034135581964516604 1101 80-81 -0.01262108983310739 1101 82-83 0.02164757257112626 1101 84-85 -0.018191798008412974 1101 86-87 -0.10331836714240339 1101 88-89 -0.06678531593824033 1101 90-91 -0.05648818307436443 1101 92-93 -0.00465433848103558 1101 94-95 -0.15943105977173389 1101 96-97 -0.180543794909223 1101 98-99 -0.4942847848149938 1101 100-101 -0.42883714555508234 1102 1 0.15370184309135482 1102 2 0.21057756020235985 1102 3 0.27800484392757596 1102 4 0.25214358367099976 1102 5 0.35953149820556973 1102 6 0.2947300682072864 1102 7 0.30249193300910093 1102 8 0.3230178002895201 1102 9 0.4038938499874263 1102 10-11 0.2853540079914154 1102 12-13 0.26075393558870985 1102 14-15 0.3543386486226652 1102 16-17 0.3885174257600639 1102 18-19 0.4887001998652565 1102 20-21 0.5656092104467589 1102 22-23 0.5573711987839189 1102 24-25 0.42596328593324984 1102 26-27 0.476582335339522 1102 28-29 0.4991469308234642 1102 30-31 0.4774243345517277 1102 32-33 0.5283809601092102 1102 34-35 0.5524473833666903 1102 36-37 0.5756595222943943 1102 38-39 0.5299988187312437 1102 40-41 0.45321997299554084 1102 42-43 0.484484100269313 1102 44-45 0.3298206374698296 1102 46-47 0.3262691351186504 1102 48-49 0.3827668038968639 1102 50-51 0.41024343825881004 1102 52-53 0.45160190058381033 1102 54-55 0.5561330697437583 1102 56-57 0.4604788746658528 1102 58-59 0.3903109024307412 1102 60-61 0.3560923401452385 1102 62-63 0.3549424294403565 1102 64-65 0.3284128019283514 1102 66-67 0.3791777569238022 1102 68-69 0.3032669933937271 1102 70-71 0.1993252874978637 1102 72-73 0.1098397386569232 1102 74-75 -0.028900600033111346 1102 76-77 0.020805270898176076 1102 78-79 0.02454625262906518 1102 80-81 0.10189622172228141 1102 82-83 -0.07993349798032412 1102 84-85 0.0803369417050952 1102 86-87 -0.05327414714049539 1102 88-89 -0.10991642084649911 1102 90-91 -0.2871604257353262 1102 92-93 -0.3751137333409993 1102 94-95 -0.5188509064976436 1102 96-97 -0.45121064568890645 1102 98-99 -0.42361826280855297 1102 100-101 -0.5879285478058627 1103 1 0.16708754122281988 1103 2 0.19150156824500897 1103 3 0.27942287924391707 1103 4 0.14933858595074412 1103 5 0.19474896054167345 1103 6 0.17055480843880844 1103 7 0.16898988764265255 1103 8 0.17278934497631582 1103 9 0.2180112030878334 1103 10-11 0.19629711893453106 1103 12-13 0.23809089089176894 1103 14-15 0.2882399435409084 1103 16-17 0.2540634387637084 1103 18-19 0.29530292680696846 1103 20-21 0.3078919696786713 1103 22-23 0.2497685860965646 1103 24-25 0.30811066977554447 1103 26-27 0.29439757857849 1103 28-29 0.30858444704063714 1103 30-31 0.38099504998685063 1103 32-33 0.34011221251842727 1103 34-35 0.21001513245986558 1103 36-37 0.18734731533812266 1103 38-39 0.2073999486747482 1103 40-41 0.25276914881590784 1103 42-43 0.28027377402509046 1103 44-45 0.09283340726226186 1103 46-47 0.26454562424260075 1103 48-49 0.24884714285855836 1103 50-51 0.2936634233737081 1103 52-53 0.3134194844352862 1103 54-55 0.3621698639161437 1103 56-57 0.39804228850044865 1103 58-59 0.3702898027486228 1103 60-61 0.3068504231067095 1103 62-63 0.37535978884076826 1103 64-65 0.21658790366278424 1103 66-67 0.16424866402861227 1103 68-69 0.14972900595743255 1103 70-71 0.1743189607966258 1103 72-73 0.11125307566348397 1103 74-75 0.08990780182614344 1103 76-77 0.10920386268659854 1103 78-79 0.2040375965271899 1103 80-81 -0.00875740927033064 1103 82-83 -0.07724585122488037 1103 84-85 0.0029379473569477454 1103 86-87 -0.14932254827364133 1103 88-89 -0.1704374847573895 1103 90-91 -0.1711965301104108 1103 92-93 -0.12295694220115294 1103 94-95 -0.15440515222138274 1103 96-97 -0.27983381668495966 1103 98-99 -0.47009629233743055 1103 100-101 -0.5546593891129774 1104 1 0.1761590037650791 1104 2 0.20702081970294017 1104 3 0.2893077660585277 1104 4 0.13530967448040343 1104 5 0.20748927291493402 1104 6 0.19933289637699403 1104 7 0.1359762830539637 1104 8 0.19484766893467764 1104 9 0.30046924911059847 1104 10-11 0.28915180255354045 1104 12-13 0.20936042464106208 1104 14-15 0.3001859011578176 1104 16-17 0.3869048133194326 1104 18-19 0.3055867103823431 1104 20-21 0.27172209968066596 1104 22-23 0.2941137555901747 1104 24-25 0.3444606060229205 1104 26-27 0.34229645137209985 1104 28-29 0.21980938938401806 1104 30-31 0.2813696461403552 1104 32-33 0.2776804928482335 1104 34-35 0.3049727772933579 1104 36-37 0.4014960000417034 1104 38-39 0.4630136807269949 1104 40-41 0.45919478374742084 1104 42-43 0.46547243253153425 1104 44-45 0.39181257334711717 1104 46-47 0.23233387717066023 1104 48-49 0.37885330762667735 1104 50-51 0.3431825797966752 1104 52-53 0.4958338894017871 1104 54-55 0.4557086533957744 1104 56-57 0.283770975346485 1104 58-59 0.416914977760932 1104 60-61 0.39690407617990076 1104 62-63 0.39535318641162576 1104 64-65 0.35283657348588804 1104 66-67 0.3185355329057842 1104 68-69 0.17716920583097107 1104 70-71 0.22100519994210543 1104 72-73 0.16761381171762224 1104 74-75 0.2598848183701463 1104 76-77 0.20512509120525024 1104 78-79 0.03830839282432663 1104 80-81 0.05541838957566014 1104 82-83 0.17180202764055252 1104 84-85 0.10741315636092708 1104 86-87 0.06802387543141108 1104 88-89 0.19881847483107506 1104 90-91 0.05922991428558788 1104 92-93 0.12233955646898309 1104 94-95 0.08317975922728849 1104 96-97 0.04201438830045845 1104 98-99 0.11708307407112528 1104 100-101 -0.15315281398554603 1105 1 0.10518400007810413 1105 2 0.12613967392960745 1105 3 0.18310891416926012 1105 4 0.17131126614143 1105 5 0.11833538544982503 1105 6 0.21546376875750184 1105 7 0.1406580482394375 1105 8 0.09392884642992527 1105 9 0.169539824807714 1105 10-11 0.214923215542278 1105 12-13 0.26276385227752286 1105 14-15 0.23623076262487075 1105 16-17 0.2727017429719041 1105 18-19 0.27347383601504305 1105 20-21 0.1844277008248767 1105 22-23 0.2553719747663479 1105 24-25 0.27062993426962123 1105 26-27 0.28805893213721134 1105 28-29 0.2813812103054474 1105 30-31 0.24135551040951952 1105 32-33 0.25465010380954567 1105 34-35 0.2589008687192589 1105 36-37 0.23935179332611511 1105 38-39 0.2942190022174813 1105 40-41 0.3067285439353 1105 42-43 0.23334105371342417 1105 44-45 0.17633589591169851 1105 46-47 0.17590573704883639 1105 48-49 0.26435249649173187 1105 50-51 0.16371932017505486 1105 52-53 0.1850189422577273 1105 54-55 0.20471873484318337 1105 56-57 0.15636922284977572 1105 58-59 0.05533731332871383 1105 60-61 0.10135808357611609 1105 62-63 0.3100393203170597 1105 64-65 0.3481826345422263 1105 66-67 0.30391632111190603 1105 68-69 0.2670069231887098 1105 70-71 0.3427137033633443 1105 72-73 0.24354884300823443 1105 74-75 0.18357621978316274 1105 76-77 0.1779673748492705 1105 78-79 0.09480748986278087 1105 80-81 0.03816820661812059 1105 82-83 0.021419277684380233 1105 84-85 0.01745578616338861 1105 86-87 -0.042984742355681504 1105 88-89 -0.16448998184207397 1105 90-91 -0.09957583906816936 1105 92-93 0.06777090495648608 1105 94-95 -0.19071115633954605 1105 96-97 -0.10768383610705001 1105 98-99 -0.33254725430635546 1105 100-101 -0.2522358001865115 1106 1 0.1934357119739829 1106 2 0.29419339979411774 1106 3 0.26271202553931516 1106 4 0.2691298361688581 1106 5 0.24615218620201063 1106 6 0.22963637714627083 1106 7 0.29396443299406627 1106 8 0.19778905941159763 1106 9 0.25153709238561106 1106 10-11 0.26879717703217665 1106 12-13 0.27950998607305166 1106 14-15 0.2656770808983282 1106 16-17 0.4208706557917168 1106 18-19 0.39306266170837745 1106 20-21 0.33914171119335634 1106 22-23 0.4415085575558422 1106 24-25 0.41236099541779936 1106 26-27 0.382611574085864 1106 28-29 0.37257861521818114 1106 30-31 0.37532983976592504 1106 32-33 0.2393336646130777 1106 34-35 0.3198608526718161 1106 36-37 0.2341925341378115 1106 38-39 0.3384826249794486 1106 40-41 0.3140751682445426 1106 42-43 0.3030319038558176 1106 44-45 0.35928045824521604 1106 46-47 0.39566906737327656 1106 48-49 0.3034547001221597 1106 50-51 0.2604895664498983 1106 52-53 0.2630010631867492 1106 54-55 0.21820457519445569 1106 56-57 0.3180315827774578 1106 58-59 0.22301107700538125 1106 60-61 0.32359229504845644 1106 62-63 0.3533966121163843 1106 64-65 0.29051637619873816 1106 66-67 0.34796958418510826 1106 68-69 0.36505829753241414 1106 70-71 0.3317462629110963 1106 72-73 0.4129288679283434 1106 74-75 0.2810558665441647 1106 76-77 0.25342527342359134 1106 78-79 0.13873209544775378 1106 80-81 0.43989125907243576 1106 82-83 0.4522535754793182 1106 84-85 0.4265986192651354 1106 86-87 0.34167842107088475 1106 88-89 0.3592506116051446 1106 90-91 0.261771582354033 1106 92-93 0.31191332672991834 1106 94-95 0.2956024103202459 1106 96-97 0.4205358222312512 1106 98-99 0.43280411962414433 1106 100-101 0.08439586037409086 1107 1 0.02952069777619215 1107 2 0.11722151761951238 1107 3 0.08098107731618143 1107 4 0.19999281347899256 1107 5 0.2195331803721956 1107 6 0.1943768132148591 1107 7 0.21833794266954243 1107 8 0.1836773297634835 1107 9 0.18007316895025127 1107 10-11 0.18602266922708566 1107 12-13 0.24405632907151897 1107 14-15 0.21406457561663927 1107 16-17 0.39475285699549545 1107 18-19 0.30647259070465793 1107 20-21 0.27137423579940645 1107 22-23 0.3767230239347157 1107 24-25 0.4489350735957629 1107 26-27 0.2893388657506861 1107 28-29 0.12112744172150514 1107 30-31 0.3112896530819995 1107 32-33 0.3407532956774233 1107 34-35 0.24728463374517418 1107 36-37 0.416838360638188 1107 38-39 0.40160534794086544 1107 40-41 0.23579933521407526 1107 42-43 0.28226874689416803 1107 44-45 0.4108503036799078 1107 46-47 0.5527413313073453 1107 48-49 0.5310425831004792 1107 50-51 0.37985329575526094 1107 52-53 0.3570848626389349 1107 54-55 0.43732013868855546 1107 56-57 0.41173329035325423 1107 58-59 0.397277475376697 1107 60-61 0.2566213214731832 1107 62-63 0.3107720379761503 1107 64-65 0.3189307890958304 1107 66-67 0.130321630232622 1107 68-69 0.23601096763255214 1107 70-71 0.3029521589082407 1107 72-73 0.4770242723363012 1107 74-75 0.4932117807132954 1107 76-77 0.43956052766889187 1107 78-79 0.4739529885048732 1107 80-81 0.4822497170242315 1107 82-83 0.3452641565987733 1107 84-85 0.35323273148232914 1107 86-87 0.28998096544451 1107 88-89 0.37983648089094046 1107 90-91 0.41569385057255914 1107 92-93 0.4373508210050012 1107 94-95 0.46739287549356234 1107 96-97 0.5974210919892222 1107 98-99 0.5024820544000121 1107 100-101 0.5414649109690366 1108 1 -0.05761040884589619 1108 2 0.05694619719599103 1108 3 0.10415518570292193 1108 4 -0.04800308835067568 1108 5 0.08046385976292925 1108 6 0.08699720422442425 1108 7 0.1156587493009198 1108 8 0.15391480306152516 1108 9 0.13847615956973414 1108 10-11 0.1625133351507415 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-0.9044355571398413 2316 60-61 -0.7768521023469432 2316 62-63 -1.1674703336759222 2316 64-65 -1.2862795409046868 2316 66-67 -1.2374648969047755 2316 68-69 -1.2255144429253946 2316 70-71 -1.1564500929006343 2316 72-73 -1.0903219087409006 2316 74-75 -1.0873829886662492 2316 76-77 -1.204318014526617 2316 78-79 -0.9610495585948868 2316 80-81 -0.9301568173659298 2316 82-83 -0.864837036652709 2316 84-85 -0.9187821971125345 2316 86-87 -0.7991515528168982 2316 88-89 -0.9502380757410158 2316 90-91 -0.9201118963752393 2316 92-93 -1.1728131863168336 2316 94-95 -1.0284033306229325 2316 96-97 -1.0761324575453948 2316 98-99 -0.9778251949049341 2316 100-101 -0.7492057965203003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8354.0 3 2279.0 4 2329.0 5 2622.0 6 2771.0 7 3450.0 8 4057.0 9 4945.0 10 5909.0 11 7172.0 12 9176.0 13 13583.0 14 11950.0 15 11830.0 16 14000.0 17 18022.0 18 17473.0 19 12528.0 20 12627.0 21 13211.0 22 14272.0 23 15637.0 24 17718.0 25 19884.0 26 22985.0 27 26988.0 28 31353.0 29 37387.0 30 45449.0 31 55941.0 32 70478.0 33 92129.0 34 128636.0 35 191941.0 36 315379.0 37 525708.0 38 530639.0 39 96830.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7786392908038 18.554442560403434 14.0440732800656 23.62284486872716 2 15.431220971450152 19.63100199431521 40.836709644932384 24.101067389302255 3 19.723072922446505 23.596192801336972 28.9562253313038 27.724508944912717 4 13.089750194216087 15.96037991813937 36.38110414123361 34.56876574641093 5 14.42425095882241 36.66499614814324 33.81988220773519 15.09087068529916 6 33.23343910612675 34.87136496581531 17.31995338545386 14.57524254260408 7 29.63507469452933 31.770647242183497 20.2437637944647 18.350514268822472 8 26.197979095375025 35.49703861497026 19.489826126019818 18.815156163634896 9 26.3784545974292 17.2252436645963 20.215829929294525 36.180471808679975 10-11 25.427827970049815 26.319324690561487 26.849557108138562 21.40329023125014 12-13 26.0669774015802 23.486533497912298 27.60465775309967 22.84183134740783 14-15 22.95397596072737 25.029285766775793 25.698009354471115 26.31872891802573 16-17 22.713705133021413 28.17067226342866 26.439298210754103 22.676324392795824 18-19 22.6135733897123 26.82901634420097 28.52655433241496 22.03085593367177 20-21 23.163168190808115 26.166324820901043 28.578526841086333 22.091980147204506 22-23 22.926451025151277 26.092519837880644 28.42618962581073 22.55483951115735 24-25 22.707957275323437 26.155187048329182 28.588512312614654 22.54834336373273 26-27 22.49600915766048 26.45067559074717 28.40593467091004 22.647380580682313 28-29 22.441457652176975 26.63424724759339 28.54406645946399 22.380228640765644 30-31 22.576466834608773 26.392349724286873 28.40071894736036 22.630464493743993 32-33 22.393884423656534 26.37178742393106 28.444121289614127 22.790206862798275 34-35 22.93833957502028 26.455443194354906 28.211795595248194 22.39442163537662 36-37 22.88765374755942 26.263455022117206 28.18634770509979 22.66254352522359 38-39 22.666193515895287 26.514172500022852 28.280759024699964 22.53887495938189 40-41 22.865214108488004 26.354220218454945 28.191447708009427 22.589117965047624 42-43 22.599012688601285 26.337169460752108 28.119487203142707 22.9443306475039 44-45 22.735948468383626 26.602295091982597 28.08130634426297 22.58045009537081 46-47 22.847987650713705 26.78043648941445 27.612118028120896 22.759457831750947 48-49 22.911702298575605 26.699499996986304 27.58781228884657 22.800985415591523 50-51 22.86166433393496 26.945306013875857 27.544563488228796 22.648466163960386 52-53 23.02377445596321 26.848860531518504 27.446167837513123 22.68119717500516 54-55 22.74637275208708 26.750516990880346 27.506699270893247 22.996410986139328 56-57 22.73468928168697 27.132020485478925 27.43720027356075 22.69608995927335 58-59 22.81208082407624 26.738942686954797 27.548392296115114 22.900584192853845 60-61 22.825813438395105 26.78381751939388 27.68142547609576 22.708943566115252 62-63 22.75037107709594 26.81331206877351 27.774061004575923 22.662255849554626 64-65 22.567811634233887 26.650954278196565 27.668747103288172 23.11248698428138 66-67 22.529828133580487 26.889135912195684 27.528765998027048 23.05226995619678 68-69 22.570108155494285 26.72212164130593 27.76812852349363 22.939641679706153 70-71 22.489267959694256 26.67143784812095 27.668438780185035 23.170855411999764 72-73 22.590666015139078 26.727090238580065 27.534176619540386 23.148067126740475 74-75 22.524441403198658 26.95956302508432 27.345291748269656 23.170703823447365 76-77 22.639281134988767 26.727347917614814 27.488814492012725 23.144556455383693 78-79 22.60626820948993 26.768487793993078 27.50550011520687 23.11974388131012 80-81 22.5053937880848 26.91631151328584 27.441894191995587 23.136400506633777 82-83 22.578500808027034 26.880068091628623 27.359481532784784 23.18194956755956 84-85 22.661329251585048 26.74632614601241 27.31596858644183 23.27637601596071 86-87 22.57648300770129 26.691624170334443 27.512187823398126 23.219704998566137 88-89 22.58325378570836 26.599063135770496 27.469005522714667 23.34867755580648 90-91 22.525192181454383 26.686417985465255 27.544297040544873 23.24409279253549 92-93 22.487008874558345 26.468660735052126 27.57911850529466 23.46521188509487 94-95 22.437783623698596 26.413753452539122 27.595432255403917 23.553030668358364 96-97 22.535480311903317 26.241566683530877 27.56934244970565 23.653610554860155 98-99 22.3295193556768 26.278784099766174 27.481652377240838 23.910044167316187 100-101 22.54513503414664 25.82253474411531 27.932743323107285 23.699586898630766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2302.0 1 1326.5 2 394.5 3 446.0 4 393.0 5 393.0 6 630.5 7 1289.5 8 2069.5 9 2441.0 10 2347.5 11 2210.5 12 2312.5 13 2536.0 14 2807.5 15 3016.0 16 3128.0 17 3288.5 18 3476.0 19 3612.0 20 3908.5 21 4447.0 22 5171.5 23 6365.0 24 7906.5 25 9683.5 26 12216.5 27 15202.5 28 17822.0 29 20869.5 30 24251.0 31 28251.5 32 33215.5 33 37302.5 34 41453.5 35 46002.5 36 51167.0 37 56416.0 38 60251.5 39 63376.0 40 66684.5 41 71629.5 42 76797.0 43 80555.0 44 83897.5 45 87727.5 46 89360.5 47 90785.5 48 95343.5 49 96328.0 50 93107.5 51 91051.0 52 89630.5 53 89036.0 54 88137.0 55 82230.5 56 73999.5 57 67096.5 58 60984.5 59 53415.5 60 44656.5 61 36968.0 62 30093.5 63 23796.5 64 17706.0 65 12715.5 66 9514.5 67 7658.0 68 5945.0 69 4260.5 70 3163.0 71 2320.5 72 1707.5 73 1387.0 74 1049.5 75 722.0 76 510.0 77 334.5 78 217.0 79 145.0 80 94.5 81 62.5 82 54.5 83 50.0 84 36.5 85 28.0 86 26.5 87 22.0 88 17.5 89 12.5 90 10.5 91 10.0 92 7.5 93 6.5 94 8.0 95 6.5 96 3.0 97 3.0 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3294894278812746 2 0.11647529863340063 3 0.11068462327520599 4 0.11763343370503954 5 0.13484001191224645 6 0.08789417954402567 7 0.0955047814433672 8 0.10030276959729989 9 0.08963138215148407 10-11 0.08832848019589028 12-13 0.09196833327818404 14-15 0.09337464015088846 16-17 0.08262052877138414 18-19 0.09755219880215744 20-21 0.1077272426458423 22-23 0.1155032924125608 24-25 0.12836686410112175 26-27 0.1277877965653023 28-29 0.12305185136163593 30-31 0.11417970947354489 32-33 0.10758247576188743 34-35 0.32847605969359056 36-37 0.4858997055027961 38-39 0.45986234737434234 40-41 0.4362653452896992 42-43 0.469334237781675 44-45 0.4775445882002581 46-47 0.48438999371298097 48-49 0.4961161113133251 50-51 0.48403841699480493 52-53 0.4880505277786969 54-55 0.47351179643294394 56-57 0.45092816253598494 58-59 0.43128122828496746 60-61 0.39070513881076074 62-63 0.4620958935839317 64-65 0.49365507428609245 66-67 0.5025065351907614 68-69 0.5164248370338507 70-71 0.5073458853115383 72-73 0.49847374342344725 74-75 0.5059809404056782 76-77 0.4824873432381456 78-79 0.46075162966149363 80-81 0.46358492439032456 82-83 0.4760969193607094 84-85 0.4765519009959962 86-87 0.47938519572482713 88-89 0.49173174282783494 90-91 0.5075526951457595 92-93 0.5002936699645941 94-95 0.504781443367195 96-97 0.5042437377982198 98-99 0.4986185103074021 100-101 0.48529995698355444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2417680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.509101310977265 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12614597184931 45.710905897031296 2 12.597494611561203 14.74136176984649 3 3.965921627207932 6.961275308331142 4 1.7501115115434076 4.095898069376032 5 0.9085308635194331 2.6578662168904086 6 0.5705252318841465 2.002855115566699 7 0.37502509625612523 1.535966694570604 8 0.26337598684164226 1.232791383759701 9 0.20738084801197715 1.0920300341660234 >10 1.1217161855542321 12.103254947252589 >50 0.07859142417437993 3.1252543928445413 >100 0.033764509131171544 3.5873829129411936 >500 0.001132905971947679 0.43479020717342304 >1k 1.4161324649314156E-4 0.20130020168192964 >5k 1.4161324649314156E-4 0.5170668485680517 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7110 0.29408358426259884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5371 0.2221551239204527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3407 0.1409202210383508 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2408590053274214E-4 0.0 0.0 0.0 0.0 7 1.2408590053274214E-4 0.0 0.0 8.272393368849475E-5 0.0 8 1.2408590053274214E-4 0.0 0.0 8.272393368849475E-5 0.0 9 1.2408590053274214E-4 0.0 0.0 1.2408590053274214E-4 0.0 10-11 1.2408590053274214E-4 0.0 0.0 1.4476688395486582E-4 0.0 12-13 1.2408590053274214E-4 0.0 0.0 2.481718010654843E-4 0.0 14-15 2.481718010654843E-4 0.0 0.0 2.8953376790973165E-4 0.0 16-17 2.8953376790973165E-4 0.0 0.0 4.5498163528672115E-4 0.0 18-19 2.8953376790973165E-4 0.0 0.0 5.790675358194633E-4 0.0 20-21 2.8953376790973165E-4 0.0 0.0 6.204295026637106E-4 0.0 22-23 2.8953376790973165E-4 0.0 0.0 8.892822871513187E-4 0.0 24-25 3.30895734753979E-4 0.0 0.0 0.0010754111379504318 0.0 26-27 3.30895734753979E-4 0.0 0.0 0.001447668839548658 0.0 28-29 4.3430065186459744E-4 0.0 0.0 0.0031228284967406766 0.0 30-31 4.7566261870884486E-4 2.0680983422123688E-5 0.0 0.007217663214321168 0.0 32-33 4.963436021309686E-4 4.1361966844247376E-5 0.0 0.015758909367658253 0.0 34-35 4.963436021309686E-4 4.1361966844247376E-5 0.0 0.02990470202839085 0.0 36-37 4.963436021309686E-4 4.1361966844247376E-5 0.0 0.05410145263227557 0.0 38-39 4.963436021309686E-4 6.204295026637107E-5 0.0 0.09099632705734423 0.0 40-41 4.963436021309686E-4 8.272393368849475E-5 0.0 0.1305176863770226 0.0 42-43 4.963436021309686E-4 8.272393368849475E-5 0.0 0.17148671453624964 0.0 44-45 5.377055689752159E-4 8.272393368849475E-5 0.0 0.22531931438403757 0.0 46-47 5.377055689752159E-4 8.272393368849475E-5 0.0 0.28719681678303166 0.0 48-49 5.377055689752159E-4 8.272393368849475E-5 0.0 0.3591873200754442 0.0 50-51 5.583865523973396E-4 8.272393368849475E-5 0.0 0.4373821183944939 0.0 52-53 5.790675358194633E-4 8.272393368849475E-5 0.0 0.5182654445584196 0.0 54-55 5.790675358194633E-4 8.272393368849475E-5 0.0 0.6081449985109693 0.0 56-57 5.99748519241587E-4 8.272393368849475E-5 0.0 0.7168442473776513 0.0 58-59 6.204295026637107E-4 8.272393368849475E-5 0.0 0.8424812216670527 0.0 60-61 6.61791469507958E-4 8.272393368849475E-5 0.0 0.9790791171701797 0.0 62-63 7.858773700407002E-4 8.272393368849475E-5 0.0 1.1165249329936138 0.0 64-65 7.858773700407002E-4 1.2408590053274214E-4 0.0 1.267392707058006 0.0 66-67 9.099632705734423E-4 1.2408590053274214E-4 0.0 1.4486822077363422 0.0 68-69 9.099632705734423E-4 1.2408590053274214E-4 0.0 1.6577255881671684 0.0 70-71 9.720062208398133E-4 1.2408590053274214E-4 0.0 1.8931785844280467 0.0 72-73 0.0010754111379504318 1.2408590053274214E-4 0.0 2.127659574468085 0.0 74-75 0.0011374540882168028 1.2408590053274214E-4 0.0 2.3721708414678533 0.0 76-77 0.0011581350716389266 1.2408590053274214E-4 0.0 2.638645312861917 0.0 78-79 0.0011581350716389266 1.2408590053274214E-4 0.0 2.941931934747361 0.0 80-81 0.0013442639224380396 1.4476688395486582E-4 0.0 3.275143939644618 0.0 82-83 0.0014063068727044109 1.654478673769895E-4 0.0 3.626968829621786 0.0 84-85 0.0014476688395486583 1.654478673769895E-4 0.0 3.9838812415207965 0.0 86-87 0.0014890308063929057 1.654478673769895E-4 0.0 4.3677823367856785 0.0 88-89 0.0014890308063929057 1.654478673769895E-4 0.0 4.790315509083088 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 39.11624 1 GTATCAA 7925 0.0 37.85784 1 ATCAACG 9645 0.0 31.044775 3 TATCAAC 9810 0.0 30.570911 2 TCAACGC 9895 0.0 30.260422 4 CAACGCA 9950 0.0 29.997921 5 AACGCAG 10240 0.0 28.906857 6 GTACATG 8370 0.0 25.352459 1 ACGCAGA 11930 0.0 24.534027 7 TACATGG 8330 0.0 24.286032 2 CGCAGAG 12090 0.0 24.248014 8 ACATGGG 8700 0.0 21.946209 3 GCAGAGT 13210 0.0 21.725641 9 GAGTACT 6880 0.0 21.133904 12-13 TACCTGG 2040 0.0 20.901913 2 GTACCTG 2240 0.0 20.557095 1 GTACTTT 7195 0.0 20.077833 14-15 GTATAGG 575 0.0 19.814425 1 GTACGCT 120 0.004836596 19.740696 3 TAGCGCG 125 0.0061235433 18.951069 4 >>END_MODULE