FastQCFastQC Report
Fri 27 May 2016
SRR939189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417680
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68310.28254359551305386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57090.2361354687138083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36860.15246020978789582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.037.051021
GTATCAA84450.036.2480131
ATCAACG101850.029.6758273
TCAACGC104050.029.048374
TATCAAC104850.028.9624962
CAACGCA104700.028.9133515
AACGCAG109600.027.6639846
ACGCAGA126750.023.7711497
CGCAGAG126300.023.6680018
GTACATG83850.023.5055351
TACATGG84050.023.089262
ACATGGG85850.021.2234173
GCAGAGT139450.021.1639279
GAGTACT74200.020.68815412-13
GTACTTT74950.020.03795614-15
AGTACTT77300.018.7228412-13
AGAGTAC124650.018.34873410-11
CAGAGTA133450.018.24106410-11
CCGATCG1851.5348571E-417.9535689
CATGGGG48400.017.9403174
Produced by FastQC (version 0.11.5)