##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939189_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2417680 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.505357201945664 34.0 31.0 34.0 31.0 34.0 2 32.711657870355054 34.0 31.0 34.0 31.0 34.0 3 32.80580556566626 34.0 31.0 34.0 31.0 34.0 4 36.26543628602627 37.0 37.0 37.0 35.0 37.0 5 36.22338812415208 37.0 37.0 37.0 35.0 37.0 6 36.25701705767513 37.0 37.0 37.0 35.0 37.0 7 36.211750107541114 37.0 37.0 37.0 35.0 37.0 8 36.19528887197644 37.0 37.0 37.0 35.0 37.0 9 37.94272070745508 39.0 38.0 39.0 35.0 39.0 10-11 37.88265465239403 39.0 38.0 39.0 35.0 39.0 12-13 37.814777596704275 39.0 38.0 39.0 35.0 39.0 14-15 39.1956760199861 40.5 39.0 41.0 36.0 41.0 16-17 39.1340214585884 40.0 38.5 41.0 36.0 41.0 18-19 39.15097179941101 40.5 38.5 41.0 36.0 41.0 20-21 39.21700928162536 41.0 39.0 41.0 36.0 41.0 22-23 39.18136498626782 41.0 39.0 41.0 36.0 41.0 24-25 39.130600410310706 41.0 39.0 41.0 36.0 41.0 26-27 39.001095885311535 40.0 39.0 41.0 35.0 41.0 28-29 38.85543909864002 40.0 38.0 41.0 35.0 41.0 30-31 38.67370847258529 40.0 38.0 41.0 35.0 41.0 32-33 38.54557054697065 40.0 38.0 41.0 34.0 41.0 34-35 38.516175837993444 40.0 38.0 41.0 34.0 41.0 36-37 38.421056756890906 40.0 38.0 41.0 34.0 41.0 38-39 38.292722155123926 40.0 38.0 41.0 34.0 41.0 40-41 38.13025193574005 40.0 38.0 41.0 33.5 41.0 42-43 37.97244651897687 40.0 38.0 41.0 33.0 41.0 44-45 37.77489039078786 40.0 37.5 41.0 33.0 41.0 46-47 37.50643281989345 40.0 37.0 41.0 33.0 41.0 48-49 37.21655864299659 40.0 37.0 41.0 32.0 41.0 50-51 36.97319020714073 40.0 36.5 41.0 31.0 41.0 52-53 36.74009835875715 39.5 36.0 41.0 31.0 41.0 54-55 36.495761639257466 39.0 36.0 41.0 31.0 41.0 56-57 36.22384951689223 39.0 35.0 40.5 30.0 41.0 58-59 35.9233779904702 39.0 35.0 40.0 29.5 41.0 60-61 35.5714519704841 38.0 35.0 40.0 29.0 41.0 62-63 35.17622658912677 38.0 34.5 40.0 28.0 41.0 64-65 34.75580928824327 37.0 34.0 40.0 28.0 41.0 66-67 34.3978491777241 36.5 34.0 39.0 27.5 41.0 68-69 33.92980646735714 36.0 33.5 39.0 26.0 40.0 70-71 33.494232900962906 35.5 33.0 39.0 26.0 40.0 72-73 33.022103835081566 35.0 33.0 38.0 25.5 40.0 74-75 32.51997762317593 35.0 32.0 37.0 24.5 39.0 76-77 30.548108724066047 33.5 29.5 35.5 20.5 37.5 78-79 31.52042060984084 34.5 31.0 36.0 22.0 38.0 80-81 31.553496327057346 35.0 32.0 36.0 23.0 37.5 82-83 31.265410434796994 35.0 31.5 35.0 21.5 37.0 84-85 30.889777389894444 34.0 31.0 35.0 20.0 36.5 86-87 30.333305482942322 34.0 31.0 35.0 17.5 36.0 88-89 29.79568222428113 34.0 30.0 35.0 9.0 36.0 90-91 29.346986987525234 34.0 29.5 35.0 4.5 35.0 92-93 29.057059453691142 34.0 29.0 35.0 2.0 35.0 94-95 28.72379285099765 34.0 29.0 35.0 2.0 35.0 96-97 28.16774407696635 34.0 29.0 35.0 2.0 35.0 98-99 27.397517868369675 34.0 28.0 35.0 2.0 35.0 100-101 23.771690422222957 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23014616621626516 1101 2 0.22296321390053464 1101 3 0.2260067768937546 1101 4 0.15213869163341087 1101 5 0.16606872091443847 1101 6 0.1837441063557108 1101 7 0.17501285514483556 1101 8 0.19000147898771758 1101 9 0.24775435157058467 1101 10-11 0.21671915097561367 1101 12-13 0.20873463077671772 1101 14-15 0.27176037205386905 1101 16-17 0.27018751772059346 1101 18-19 0.3400733909258591 1101 20-21 0.3770463438791296 1101 22-23 0.2534487264065035 1101 24-25 0.15446977515824045 1101 26-27 0.3234112921765302 1101 28-29 0.2745548647351157 1101 30-31 0.22790206813832725 1101 32-33 0.3009054745913602 1101 34-35 0.41779495806923705 1101 36-37 0.46506212996713003 1101 38-39 0.5018670425935241 1101 40-41 0.4495859855776274 1101 42-43 0.47345797426952885 1101 44-45 0.6178128867119028 1101 46-47 0.612731840738526 1101 48-49 0.5606356174210845 1101 50-51 0.5825914780038914 1101 52-53 0.6936093877709055 1101 54-55 0.7036388359543508 1101 56-57 0.677754281560965 1101 58-59 0.6135046969236484 1101 60-61 0.5531901535633494 1101 62-63 0.4839363135422161 1101 64-65 0.5401690552003089 1101 66-67 0.48425965995503617 1101 68-69 0.5553190343355965 1101 70-71 0.5399377867326152 1101 72-73 0.5527882654313245 1101 74-75 0.5995422256997358 1101 76-77 0.5613545632823396 1101 78-79 0.7587368540915804 1101 80-81 0.6530188726126092 1101 82-83 0.7913616909580448 1101 84-85 0.6730840758210945 1101 86-87 0.7032370536096799 1101 88-89 0.8285465092383184 1101 90-91 0.6853183480104654 1101 92-93 0.6959808394183113 1101 94-95 0.7019356201780234 1101 96-97 0.5965149437203223 1101 98-99 0.7005153981926426 1101 100-101 0.33702254760019557 1102 1 0.16465782287178854 1102 2 0.13809829038962818 1102 3 0.1780716372801905 1102 4 0.1042949509828972 1102 5 0.16781582195632438 1102 6 0.17112644662945087 1102 7 0.162319577571445 1102 8 0.11908048648703584 1102 9 0.1681747868006127 1102 10-11 0.23764030786291812 1102 12-13 0.2778611788877541 1102 14-15 0.27213550233027206 1102 16-17 0.2708207349969669 1102 18-19 0.29033624513793654 1102 20-21 0.30284485190613 1102 22-23 0.3200595016209462 1102 24-25 0.3079125242286338 1102 26-27 0.2128053959570977 1102 28-29 0.1954309796873943 1102 30-31 0.2846789067205293 1102 32-33 0.32414481165297815 1102 34-35 0.33628648065103306 1102 36-37 0.4475648176884377 1102 38-39 0.36068720691439893 1102 40-41 0.43077522417165426 1102 42-43 0.3784099569366006 1102 44-45 0.4170928732979604 1102 46-47 0.33473203142701635 1102 48-49 0.4639976526590033 1102 50-51 0.4450219013652088 1102 52-53 0.3563228848990718 1102 54-55 0.38939868024733926 1102 56-57 0.4135876066749731 1102 58-59 0.29763283958494213 1102 60-61 0.3986940150051481 1102 62-63 0.4830318583359343 1102 64-65 0.4022467048661085 1102 66-67 0.41420597128357173 1102 68-69 0.33586519245580604 1102 70-71 0.26249985090257866 1102 72-73 0.38561134268317687 1102 74-75 0.4042555189884922 1102 76-77 0.49590468640792196 1102 78-79 0.3733508249819977 1102 80-81 0.3879691127814695 1102 82-83 0.2518030361666739 1102 84-85 0.27633498570105886 1102 86-87 0.3721824476608582 1102 88-89 0.4109515513048585 1102 90-91 0.679401087086152 1102 92-93 0.6179751121883257 1102 94-95 0.365905688661492 1102 96-97 0.3717017691187152 1102 98-99 0.14048633463748672 1102 100-101 -0.23595722298182764 1103 1 0.07443639366731247 1103 2 0.09653234373893582 1103 3 0.11856113709172433 1103 4 0.10187681636137569 1103 5 0.08289728958015985 1103 6 0.06872315439564858 1103 7 0.08775483859992761 1103 8 0.12212776750042309 1103 9 0.12613025323065585 1103 10-11 0.1516214099796116 1103 12-13 0.13601312009054567 1103 14-15 0.2531840155821712 1103 16-17 0.153021081604102 1103 18-19 0.17604259864089755 1103 20-21 0.20630569096018547 1103 22-23 0.12872959164697306 1103 24-25 0.14361679552612117 1103 26-27 0.22792647887478523 1103 28-29 0.253270162950308 1103 30-31 0.18301057962846556 1103 32-33 0.189501931247392 1103 34-35 0.18695059741683906 1103 36-37 0.24773182124526727 1103 38-39 0.2862737748738411 1103 40-41 0.28249816911494463 1103 42-43 0.3719623809974948 1103 44-45 0.3970288481491835 1103 46-47 0.3128046993301936 1103 48-49 0.33365052231187065 1103 50-51 0.3851858296647279 1103 52-53 0.4183547757445183 1103 54-55 0.39301819403634397 1103 56-57 0.4781385390225168 1103 58-59 0.39717325946434556 1103 60-61 0.4276728694077292 1103 62-63 0.46895582900566524 1103 64-65 0.3852539861784763 1103 66-67 0.29319107230256947 1103 68-69 0.2460149297241898 1103 70-71 0.13043901206207664 1103 72-73 0.2513607711444763 1103 74-75 0.2976681683333453 1103 76-77 0.253067313062072 1103 78-79 0.321912661679427 1103 80-81 0.21893791493671344 1103 82-83 0.2312307393231876 1103 84-85 0.15851440347369916 1103 86-87 0.17230919524523358 1103 88-89 0.2673537992325272 1103 90-91 0.2387926346132936 1103 92-93 0.2694513958679998 1103 94-95 0.1928524173031363 1103 96-97 0.14124038442004405 1103 98-99 0.0421784736177635 1103 100-101 -0.4332561219756421 1104 1 0.10860771586627038 1104 2 0.09730596169921313 1104 3 0.0903207469815328 1104 4 0.014706619993290815 1104 5 0.040223880988584426 1104 6 0.09784261793160454 1104 7 0.10608345700411093 1104 8 0.012519107314190592 1104 9 0.10144533790555954 1104 10-11 0.11600101297521093 1104 12-13 0.043195950273478445 1104 14-15 0.08736623762469975 1104 16-17 0.12317093257009759 1104 18-19 0.19256002984580078 1104 20-21 0.08267478842396514 1104 22-23 0.14379065369605115 1104 24-25 0.043385543778086344 1104 26-27 0.13953895963333451 1104 28-29 0.10156497308253876 1104 30-31 0.18016494934032323 1104 32-33 0.1618076795706287 1104 34-35 0.24405535720971017 1104 36-37 0.15968974159680016 1104 38-39 0.16155809823114708 1104 40-41 0.2802076388907011 1104 42-43 0.13813197589428938 1104 44-45 0.06401007499222544 1104 46-47 0.1263431803630013 1104 48-49 -0.013446347077994858 1104 50-51 0.0417548923657165 1104 52-53 0.09195010269189652 1104 54-55 0.19592463836565344 1104 56-57 0.15062700040225963 1104 58-59 0.17373105135194322 1104 60-61 0.23612187377058547 1104 62-63 0.16384117640639317 1104 64-65 0.020810943175568752 1104 66-67 0.03559859140231225 1104 68-69 -0.11137854045671247 1104 70-71 -0.09420968160313237 1104 72-73 -0.2068849084118014 1104 74-75 -0.052595803786395834 1104 76-77 -0.1594822833102576 1104 78-79 0.018837822061350096 1104 80-81 0.08028221369329813 1104 82-83 -0.13644099156780243 1104 84-85 -0.07327379193319317 1104 86-87 -0.06899177090189212 1104 88-89 -0.06671494355908791 1104 90-91 -0.0017499275043526552 1104 92-93 0.04836224098309927 1104 94-95 0.004415692458120901 1104 96-97 -0.10164303893947135 1104 98-99 -0.1913718284308672 1104 100-101 -0.49940587623940047 1105 1 -0.018981168106648738 1105 2 0.03322212679787384 1105 3 0.024876208855403092 1105 4 0.014251660939606836 1105 5 0.04580999903306804 1105 6 0.011361440992885719 1105 7 0.12300598596427648 1105 8 0.06893124734697409 1105 9 0.02919450103133414 1105 10-11 0.031652717471651215 1105 12-13 0.030485618668151915 1105 14-15 0.07767644456846057 1105 16-17 0.05141262017601633 1105 18-19 0.11915788221644874 1105 20-21 0.009217683967030155 1105 22-23 0.10094589351651706 1105 24-25 0.07221213898696277 1105 26-27 0.09591158640273534 1105 28-29 -0.0236044191645135 1105 30-31 0.035275099518550235 1105 32-33 -0.0607653851838208 1105 34-35 0.13250830164349026 1105 36-37 0.044884928770009935 1105 38-39 0.0818247849327065 1105 40-41 0.043451538682830915 1105 42-43 0.059346146129676924 1105 44-45 0.1235359714841735 1105 46-47 0.00535468094874858 1105 48-49 0.023459597442041513 1105 50-51 0.05625653828791144 1105 52-53 0.002121428862750463 1105 54-55 0.030023193348910127 1105 56-57 0.02911397632977497 1105 58-59 0.09039145711420105 1105 60-61 0.016035083722364618 1105 62-63 -0.10123647272138925 1105 64-65 -0.19447095056714403 1105 66-67 -0.21184626394297368 1105 68-69 -0.47004418868293385 1105 70-71 -0.3031986707592438 1105 72-73 -0.2813534967587259 1105 74-75 -0.29408375641500584 1105 76-77 -0.16167290419719293 1105 78-79 -0.1324284110724676 1105 80-81 -0.26890581657052337 1105 82-83 -0.042268641391245865 1105 84-85 -0.1210361446975945 1105 86-87 -0.013237025832008698 1105 88-89 0.2207830042555763 1105 90-91 0.12449147070864441 1105 92-93 0.01100898962587138 1105 94-95 -0.04557762924384434 1105 96-97 -0.11341006094724548 1105 98-99 -0.31143788792893545 1105 100-101 -0.39009605051515095 1106 1 0.09216425166798814 1106 2 0.0714867420068046 1106 3 0.04927624236270134 1106 4 0.008846919507348616 1106 5 0.04991220078528613 1106 6 0.049108796752811656 1106 7 0.05252816520049919 1106 8 0.035325347959755504 1106 9 0.050039267977219026 1106 10-11 0.09011411665658642 1106 12-13 0.05116829596099137 1106 14-15 0.09608028783997469 1106 16-17 0.041905583080954045 1106 18-19 -0.00861891263063086 1106 20-21 0.11139530117025487 1106 22-23 0.09705583575399856 1106 24-25 0.095004423444351 1106 26-27 0.13742963958392096 1106 28-29 0.12748727249175573 1106 30-31 0.07343988778588795 1106 32-33 0.17857642188854328 1106 34-35 0.027358320820034976 1106 36-37 0.09349398167434941 1106 38-39 0.16288933267627925 1106 40-41 0.1022331729296937 1106 42-43 0.1565519496529717 1106 44-45 0.10817924465088424 1106 46-47 0.14602436477438374 1106 48-49 0.17091319340944722 1106 50-51 0.16769796419603722 1106 52-53 0.18426023845981376 1106 54-55 0.23922731249213314 1106 56-57 0.18680974137603812 1106 58-59 0.12014505079018534 1106 60-61 0.1619617876452395 1106 62-63 0.259073142932678 1106 64-65 0.24412327197913442 1106 66-67 0.24486997623820628 1106 68-69 0.15741443713488934 1106 70-71 0.1314212562384327 1106 72-73 0.10861120037779415 1106 74-75 0.14728496016927295 1106 76-77 0.24258826937697364 1106 78-79 0.45951182649123723 1106 80-81 0.34558084756431384 1106 82-83 0.3341435388855061 1106 84-85 0.38808520166452 1106 86-87 0.35056164942785273 1106 88-89 0.5878616002699353 1106 90-91 0.5476520536604177 1106 92-93 0.5152548745583694 1106 94-95 0.369556322922616 1106 96-97 0.17226187109756097 1106 98-99 0.06764050749243466 1106 100-101 -0.3341530512292472 1107 1 0.02033393370253833 1107 2 0.02269384946972508 1107 3 0.04638202105449096 1107 4 0.04597723776827678 1107 5 0.11409883447644376 1107 6 0.07761960982364258 1107 7 0.0479929248427311 1107 8 0.08666453474118896 1107 9 0.18663055783370197 1107 10-11 0.10773318749188121 1107 12-13 0.09894954069776674 1107 14-15 0.0934536601174969 1107 16-17 0.10659951806956514 1107 18-19 0.1349277790335961 1107 20-21 0.17187255959363057 1107 22-23 0.15457756088127184 1107 24-25 0.1167066510639998 1107 26-27 0.1508220614222111 1107 28-29 0.09247517260787674 1107 30-31 0.2609192042237112 1107 32-33 0.16676610788253043 1107 34-35 0.19009658806938745 1107 36-37 0.24079049235741223 1107 38-39 0.20650191626003078 1107 40-41 0.15678182066794477 1107 42-43 0.22923361781180773 1107 44-45 0.20748379680743767 1107 46-47 0.08035479354462183 1107 48-49 0.10718307548359718 1107 50-51 0.11621750129791764 1107 52-53 0.14906974285212016 1107 54-55 0.12001741718050596 1107 56-57 0.09880857127738096 1107 58-59 0.08336265959310651 1107 60-61 0.12349996506883087 1107 62-63 0.043036844758447046 1107 64-65 0.02073101265454369 1107 66-67 0.026301049744631655 1107 68-69 0.011267710899559802 1107 70-71 0.08897895598993699 1107 72-73 -0.1572154181848191 1107 74-75 -0.039749659681632465 1107 76-77 0.06145271226522553 1107 78-79 0.26126025232441563 1107 80-81 0.1978623552778025 1107 82-83 0.16026403393179223 1107 84-85 0.19965942758220478 1107 86-87 0.11839937506335474 1107 88-89 0.10294804278588643 1107 90-91 0.18020672474746036 1107 92-93 0.09631781759288671 1107 94-95 -0.017175319677246392 1107 96-97 -0.014526392753776207 1107 98-99 0.10604397287553624 1107 100-101 -0.021030697617160143 1108 1 -0.006079238490272587 1108 2 -0.011446927905588211 1108 3 0.07313912609767925 1108 4 0.05159480765775726 1108 5 0.060451114591614896 1108 6 0.06719858957659142 1108 7 0.05053541578728016 1108 8 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82-83 0.08236962200964015 2316 84-85 -0.08809204455511477 2316 86-87 -0.22166909443188487 2316 88-89 -0.5297437667773934 2316 90-91 -0.6120608847778293 2316 92-93 -0.5894559826799544 2316 94-95 -0.2701627654974139 2316 96-97 -0.3871331982589652 2316 98-99 -0.3231043460386225 2316 100-101 -0.05588085044314539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 10.0 5 30.0 6 81.0 7 216.0 8 480.0 9 943.0 10 1547.0 11 2443.0 12 3796.0 13 5456.0 14 6884.0 15 9295.0 16 12022.0 17 16810.0 18 16946.0 19 12073.0 20 10888.0 21 11520.0 22 12263.0 23 13471.0 24 15618.0 25 18199.0 26 21756.0 27 26345.0 28 32527.0 29 39602.0 30 48596.0 31 60182.0 32 75090.0 33 98017.0 34 136583.0 35 200961.0 36 331525.0 37 583958.0 38 539006.0 39 52531.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00174562079936 19.084042967339542 14.515795668569446 23.398415743291643 2 15.765485920386485 20.056169550974488 40.199943747725094 23.978400780913933 3 19.72324707984514 23.808113563416168 29.164612355646735 27.304027001091956 4 13.411783197114588 16.39815856523609 36.044389662817245 34.14566857483207 5 14.321994639489096 36.921139274014756 33.80658317064293 14.950282915853213 6 33.145618940471856 35.28341219681678 17.03351973793058 14.537449124780782 7 29.58683531319281 31.941861619403728 20.123630918897454 18.347672148506007 8 26.162312630290195 35.94867807153966 19.261564805929652 18.627444492240492 9 26.15213758644651 17.269820654511765 20.144394626253266 36.43364713278846 10-11 25.36040749809735 26.605774957810794 26.710172562125674 21.323644981966183 12-13 25.991156811488704 23.63226316137785 27.580676516329707 22.795903510803743 14-15 22.82299938928437 25.18084152187936 25.72440531746562 26.271753771370655 16-17 22.615221073487106 28.40639716086175 26.410727839396763 22.567653926254376 18-19 22.521133914441872 26.975655980655823 28.54248994282944 21.960720162072864 20-21 23.025834684490917 26.246008570199532 28.691203964130903 22.036952781178652 22-23 22.87424307600675 26.07545249991728 28.594437642698782 22.45586678137719 24-25 22.677490584428277 26.252316020731527 28.671586967703 22.398606427137196 26-27 22.480946405068718 26.537109942117443 28.502527656976557 22.479415995837286 28-29 22.383193805631844 26.685148572184904 28.663967108963966 22.267690513219286 30-31 22.579989353090642 26.442017155008497 28.49034767081278 22.48764582108808 32-33 22.361685582872838 26.453066576221833 28.507763641176666 22.677484199728664 34-35 22.85157316477898 26.54371189118978 28.30215684490076 22.30255809913048 36-37 22.82082646276197 26.32846389022146 28.283357360939327 22.56735228607725 38-39 22.60744085351563 26.53116442032794 28.401813619355476 22.45958110680095 40-41 22.841509958474983 26.356326670080893 28.306529744702647 22.495633626741483 42-43 22.575014017977345 26.42643648847961 28.175255431229385 22.82329406231366 44-45 22.680563325519135 26.602705360568248 28.151447246916632 22.565284066995986 46-47 22.803091014941145 26.844614765769688 27.682907282994556 22.669386936294607 48-49 22.89290231637011 26.770429447954264 27.601831852426024 22.7348363832496 50-51 22.868900889841225 26.995586882209526 27.54952736685213 22.58598486109712 52-53 23.03461294903087 26.94346519947437 27.379730725212077 22.642191126282686 54-55 22.712124846960723 26.85669732967142 27.522170014228514 22.90900780913934 56-57 22.73088249892459 27.195948181727935 27.43073938651931 22.642429932828165 58-59 22.796814499133227 26.814697678958993 27.48594771877155 22.90254010313624 60-61 22.845118308223736 26.81173913430162 27.662806308586102 22.680336248888544 62-63 22.74121982464261 26.892007989817905 27.686832725493332 22.679939460046153 64-65 22.571594731382227 26.706563767250323 27.654328443156867 23.067513058210583 66-67 22.49756161321519 26.953522155608372 27.499046568017132 23.049869663159306 68-69 22.53446352104418 26.848486665970384 27.649779687850618 22.96727012513482 70-71 22.51292355723136 26.77209289243646 27.551766612427297 23.16321693790488 72-73 22.630372919493066 26.837153800337514 27.407700770987063 23.124772509182357 74-75 22.525933953211343 27.066733397306507 27.16713957182092 23.240193077661228 76-77 22.628036917526252 26.807093261915178 27.39698941971437 23.1678804008442 78-79 22.605453761078326 26.875761838562816 27.36683361797366 23.151950782385196 80-81 22.487033687388085 27.02831431524757 27.347306907521517 23.137345089842825 82-83 22.58905903462265 26.99101116531398 27.24758093136107 23.172348868702294 84-85 22.630170789800715 26.91307925636959 27.146960546259336 23.309789407570356 86-87 22.52070021198657 26.84045189846922 27.34463169223004 23.294216197314174 88-89 22.514020608966213 26.791739136935167 27.268236343611235 23.426003910487385 90-91 22.474436566350736 26.893355064414664 27.305027561793437 23.327180807441163 92-93 22.420067999073492 26.7419799146289 27.307501406306873 23.530450679990736 94-95 22.271206280401046 26.681157142384436 27.333559445418746 23.714077131795772 96-97 22.435868270407994 26.534694417788955 27.301359154230504 23.72807815757255 98-99 22.16953329453531 26.59097743303515 27.157767732205958 24.08172154022358 100-101 22.377319102795283 26.12947329954177 27.664156875364633 23.829050722298316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 416.0 1 288.5 2 235.5 3 313.5 4 280.5 5 275.5 6 506.5 7 1090.5 8 1780.5 9 2111.5 10 1972.5 11 1804.5 12 1881.0 13 2043.5 14 2213.0 15 2317.5 16 2459.0 17 2613.5 18 2796.5 19 3141.5 20 3498.5 21 4079.5 22 4958.5 23 6218.0 24 7790.5 25 9634.5 26 12133.5 27 15225.5 28 18027.0 29 20934.5 30 24562.0 31 28817.0 32 33717.0 33 37956.0 34 42208.5 35 46610.5 36 51355.5 37 56781.5 38 60815.5 39 63939.0 40 67338.5 41 72083.5 42 77313.5 43 81253.5 44 84986.5 45 89001.5 46 90507.0 47 91936.0 48 96184.0 49 96908.5 50 93724.0 51 91376.0 52 89651.5 53 89275.0 54 88663.0 55 82704.5 56 74318.0 57 67307.5 58 60956.5 59 53201.5 60 44625.0 61 36821.0 62 29633.0 63 23375.0 64 17241.0 65 12197.0 66 9093.5 67 7195.5 68 5416.5 69 3816.0 70 2787.0 71 2017.5 72 1455.0 73 1210.0 74 942.5 75 619.0 76 395.5 77 214.0 78 124.5 79 81.0 80 46.0 81 24.0 82 19.0 83 13.5 84 10.0 85 6.0 86 3.5 87 2.5 88 3.5 89 4.0 90 2.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34007809139340195 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010133681876840607 16-17 0.001861288507991132 18-19 8.272393368849475E-5 20-21 0.0 22-23 0.0 24-25 3.929386850203501E-4 26-27 0.0010754111379504318 28-29 0.0 30-31 0.003184871447007048 32-33 0.0 34-35 0.010754111379504317 36-37 0.02765047483537937 38-39 0.02169435160980775 40-41 0.014145792660732601 42-43 0.010278448760795473 44-45 0.002998742596207935 46-47 0.0011374540882168028 48-49 7.445154031964529E-4 50-51 6.204295026637107E-5 52-53 4.1361966844247376E-5 54-55 0.0 56-57 0.0 58-59 0.004177558651268985 60-61 0.010113000893418484 62-63 0.00426028258495748 64-65 0.0018199265411468848 66-67 0.004053472750736243 68-69 0.0036191720988716456 70-71 8.272393368849475E-5 72-73 0.0 74-75 0.0 76-77 0.2159715098772377 78-79 3.722577015982264E-4 80-81 0.002853975712253069 82-83 5.790675358194633E-4 84-85 2.0680983422123688E-5 86-87 0.11037440852387412 88-89 0.4051818272062473 90-91 0.39107739651235895 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012822209721716686 100-101 6.411104860858343E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2417680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.000349934437345 #Duplication Level Percentage of deduplicated Percentage of total 1 76.91273154407267 43.840526124255796 2 13.22590014786656 15.077618732526412 3 4.2239160195383505 7.222940736220849 4 1.8662556736990399 4.255089058718975 5 0.9833472251428894 2.802556797010132 6 0.5850468561441208 2.000872531695439 7 0.3822948309329837 1.5253657398914644 8 0.2715883629963726 1.2384505383131388 9 0.2160508055053995 1.108347436568235 >10 1.2102864689216022 12.685310715715028 >50 0.08395881412337634 3.2514745119009367 >100 0.03688396373871283 3.7291885168047885 >500 0.0013769357934030081 0.4846292970641088 >1k 2.174109147471284E-4 0.2596228377121937 >5k 1.4494060983141893E-4 0.5180064256023721 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6831 0.28254359551305386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5709 0.2361354687138083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3686 0.15246020978789582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.272393368849475E-5 0.0 5 4.1361966844247376E-5 0.0 0.0 8.272393368849475E-5 0.0 6 1.654478673769895E-4 0.0 0.0 8.272393368849475E-5 0.0 7 2.481718010654843E-4 0.0 0.0 8.272393368849475E-5 0.0 8 2.481718010654843E-4 0.0 0.0 8.272393368849475E-5 0.0 9 2.481718010654843E-4 0.0 0.0 8.272393368849475E-5 0.0 10-11 2.481718010654843E-4 0.0 0.0 1.0340491711061845E-4 0.0 12-13 2.481718010654843E-4 0.0 0.0 2.2749081764336058E-4 0.0 14-15 2.481718010654843E-4 0.0 0.0 2.481718010654843E-4 0.0 16-17 3.30895734753979E-4 0.0 0.0 3.30895734753979E-4 0.0 18-19 3.30895734753979E-4 0.0 0.0 4.136196684424738E-4 0.0 20-21 3.30895734753979E-4 8.272393368849475E-5 0.0 4.7566261870884486E-4 0.0 22-23 3.515767181761027E-4 8.272393368849475E-5 0.0 8.892822871513187E-4 0.0 24-25 4.136196684424738E-4 8.272393368849475E-5 0.0 0.0010960921213725556 0.0 26-27 4.136196684424738E-4 8.272393368849475E-5 0.0 0.001447668839548658 0.0 28-29 4.3430065186459744E-4 8.272393368849475E-5 0.0 0.0031641904635849247 0.0 30-31 4.5498163528672115E-4 8.272393368849475E-5 0.0 0.007321068131431786 0.0 32-33 4.5498163528672115E-4 8.272393368849475E-5 0.0 0.01602776215214586 0.0 34-35 4.7566261870884486E-4 8.272393368849475E-5 0.0 0.030401045630521824 0.0 36-37 4.963436021309686E-4 8.272393368849475E-5 0.0 0.054680520168095034 0.0 38-39 5.790675358194633E-4 8.272393368849475E-5 0.0 0.09174084246054068 0.0 40-41 5.790675358194633E-4 8.272393368849475E-5 0.0 0.1319033122663049 0.0 42-43 6.204295026637106E-4 8.272393368849475E-5 0.0 0.1736375368121505 0.0 44-45 6.61791469507958E-4 8.272393368849475E-5 0.0 0.22798716124549154 0.0 46-47 7.031534363522054E-4 8.272393368849475E-5 0.0 0.2904437311803051 0.0 48-49 7.031534363522054E-4 8.272393368849475E-5 0.0 0.36305466397538133 0.0 50-51 7.031534363522054E-4 8.272393368849475E-5 0.0 0.44232487343238147 0.0 52-53 7.031534363522054E-4 8.272393368849475E-5 0.0 0.5234563713973727 0.0 54-55 7.031534363522054E-4 8.272393368849475E-5 0.0 0.6136668210846763 0.0 56-57 7.031534363522054E-4 8.272393368849475E-5 0.0 0.722490155851891 0.0 58-59 7.031534363522054E-4 8.272393368849475E-5 0.0 0.848561430793157 0.0 60-61 8.065583534628239E-4 8.272393368849475E-5 0.0 0.9857590748155256 0.0 62-63 8.686013037291949E-4 8.272393368849475E-5 0.0 1.1245077925945535 0.0 64-65 8.686013037291949E-4 1.2408590053274214E-4 0.0 1.2767818735316503 0.0 66-67 8.892822871513186E-4 1.2408590053274214E-4 0.0 1.4586090797789617 0.0 68-69 9.099632705734423E-4 1.2408590053274214E-4 0.0 1.6686037854472056 0.0 70-71 9.099632705734423E-4 1.654478673769895E-4 0.0 1.9043669964594157 0.0 72-73 9.099632705734423E-4 1.654478673769895E-4 0.0 2.139695906819761 0.0 74-75 9.099632705734423E-4 1.654478673769895E-4 0.0 2.3867716157638728 0.0 76-77 9.099632705734423E-4 1.654478673769895E-4 0.0 2.6547971609145957 0.0 78-79 9.099632705734423E-4 1.654478673769895E-4 0.0 2.959303960821945 0.0 80-81 0.0010340491711061843 1.654478673769895E-4 0.0 3.294625426028259 0.0 82-83 0.001054730154528308 1.654478673769895E-4 0.0 3.6486011382813275 0.0 84-85 0.0011167731047946792 1.654478673769895E-4 0.0 4.007602329505973 0.0 86-87 0.0011581350716389266 1.654478673769895E-4 0.0 4.391834320505609 0.0 88-89 0.0011581350716389266 1.654478673769895E-4 0.0 4.815773799675722 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5705 0.0 37.05102 1 GTATCAA 8445 0.0 36.248013 1 ATCAACG 10185 0.0 29.675827 3 TCAACGC 10405 0.0 29.04837 4 TATCAAC 10485 0.0 28.962496 2 CAACGCA 10470 0.0 28.913351 5 AACGCAG 10960 0.0 27.663984 6 ACGCAGA 12675 0.0 23.771149 7 CGCAGAG 12630 0.0 23.668001 8 GTACATG 8385 0.0 23.505535 1 TACATGG 8405 0.0 23.08926 2 ACATGGG 8585 0.0 21.223417 3 GCAGAGT 13945 0.0 21.163927 9 GAGTACT 7420 0.0 20.688154 12-13 GTACTTT 7495 0.0 20.037956 14-15 AGTACTT 7730 0.0 18.72284 12-13 AGAGTAC 12465 0.0 18.348734 10-11 CAGAGTA 13345 0.0 18.241064 10-11 CCGATCG 185 1.5348571E-4 17.953568 9 CATGGGG 4840 0.0 17.940317 4 >>END_MODULE