FastQCFastQC Report
Fri 27 May 2016
SRR939188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375770
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67830.28550743548407465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52370.22043379620081066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.1360401048923086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53750.035.693881
GTATCAA79400.035.4072381
ATCAACG97050.028.7024423
TCAACGC98750.028.2083264
TATCAAC100050.027.889152
CAACGCA99600.027.8712925
AACGCAG104050.026.7107496
GTACATG86150.024.0889021
TACATGG86250.023.3984762
ACGCAGA119650.023.1094827
CGCAGAG121000.022.8115588
ACATGGG87700.022.3093833
GAGTACT65600.021.43662512-13
GCAGAGT132400.020.4540759
ATTCGCG2000.020.21090180-81
GTACTTT68950.019.91462314-15
CATGGGG50850.018.819044
AGTACTT70300.018.42068512-13
AGAGTAC117000.018.0279910-11
CAGAGTA126800.017.54947310-11
Produced by FastQC (version 0.11.5)